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Reducing manual labour in forensic microtrace recognition with deep learning 利用深度学习减少法医微迹识别中的体力劳动。
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-12-02 DOI: 10.1016/j.forsciint.2025.112714
Gerben Rijpkema , Dylan Kalisvaart , Serafim Korovin , Daniel Spengler , Anna Pals , Jaap van der Weerd , Carlas S. Smith
Forensic microtrace investigation relies on time- and labour-intensive microscopic analyses. To aid forensic experts in their investigations, an image recognition model for microtrace localisation and classification is needed. In this work, we use deep learning to automate trace recognition in images captured with automated microscopy. We localise and classify fibres, hairs, skin, glass and sand in microscopy scans through pixel-wise classification of tape-lift samples. As deep learning requires extensive amounts of annotated training data, we additionally investigate various pretraining strategies to minimise the required annotation workload. We compare ImageNet pretraining, pretraining with self-supervised learning and a sequential application of these approaches. We find that pretrained models are able to reduce the required annotated data twofold compared to models trained from scratch while retaining the prediction accuracy. While our ImageNet-pretrained models outperform our self-supervised-pretrained models, we achieve the highest accuracy by combining the two approaches, resulting in a factor 4 reduction of manual annotated microtraces or a 65 % improvement in recognition and localisation accuracy (mean intersection over union increases from 0.34 to 0.56 due to pretraining) when training on only 2.2 dm2 of annotated tape lift scans. The developed models offer a solid fundament for automated analysis of forensic microtrace scans.
法医微迹调查依赖于时间和劳动密集的显微分析。为了帮助法医专家进行调查,需要一种用于微迹定位和分类的图像识别模型。在这项工作中,我们使用深度学习来自动识别自动显微镜捕获的图像中的痕迹。我们在显微镜扫描中对纤维、头发、皮肤、玻璃和沙子进行定位和分类,通过对胶带提升样本进行像素级分类。由于深度学习需要大量带注释的训练数据,我们还研究了各种预训练策略,以尽量减少所需的注释工作量。我们比较了ImageNet预训练、预训练与自监督学习以及这些方法的顺序应用。我们发现,与从头开始训练的模型相比,预训练模型能够在保持预测精度的同时将所需的注释数据减少两倍。虽然我们的imagenet预训练模型优于我们的自我监督预训练模型,但我们通过结合两种方法实现了最高的精度,当仅在2.2 dm2的带注释的胶带提升扫描上训练时,导致手动注释微迹减少了4倍,识别和定位精度提高了65%(由于预训练,平均交集从0.34增加到0.56)。开发的模型为法医微迹扫描的自动分析提供了坚实的基础。
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引用次数: 0
Deep learning method based on image recognition for intra-puparial age and postmortem interval estimation in the forensically important Sarcophaga peregrina (Diptera: Sarcophagidae) 基于图像识别的深度学习方法在具有重要法医意义的佩雷戈石棺(双翅目:石棺科)蛹内年龄和死后时间估计中的应用
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-12-02 DOI: 10.1016/j.forsciint.2025.112761
Gang Yu , Bingqian Bai , Maoxu Zhou , Mingxing Zhang , Bo Xuan , Mingyuan Zhang , Xiangyan Zhang , Yanjie Shang
Accurate estimation of intra-puparial age in necrophagous flies is essential for determining the postmortem interval (PMI) in forensic entomology. Traditional methods based on morphological observation of intra-pupal structures are widely used but rely on complex diagnostic criteria and are subject to observer bias, posing a technical bottleneck in PMI estimation using insect evidence. Deep learning, particularly image-based methods, offers a promising solution for objective and automated identification in forensic entomology. Sarcophaga peregrina (Robineau-Desvoidy, 1830) (Diptera: Sarcophagidae) is a common necrophagous fly species. In this study, we propose an image-based deep learning framework for automatic classification of intra-pupal developmental age in S. peregrina to enhance the accuracy of PMI estimation. Pupae were reared at 25 °C, and samples from different developmental stages (Day 1 to Day 11) were collected. After removing the puparium, high-resolution images of intra-pupal morphology were captured to construct a dataset. A ResNet50 network was first employed to extract regions of interest, followed by a Vision Transformer (ViT) model for end-to-end classification of developmental stages. The proposed method achieved a classification precision of 94.00 %, recall of 93.41 %, and F1-score of 93.43 %. These findings demonstrate that deep learning can serve as an effective and objective alternative to manual morphological assessment, reducing reliance on expert experience in intra-puparial age estimation. The proposed approach establishes a viable AI-assisted pathway for standardized, rapid, and accurate PMI inference based on insect evidence, offering practical value for forensic investigations.
尸食性蝇蛹内年龄的准确估计是法医昆虫学中确定死后时间间隔(PMI)的关键。基于蛹内结构形态学观察的传统方法被广泛使用,但依赖于复杂的诊断标准,并且容易受到观察者的偏见,这给利用昆虫证据进行PMI估计带来了技术瓶颈。深度学习,特别是基于图像的方法,为法医昆虫学的客观和自动鉴定提供了一个很有前途的解决方案。peregrina Sarcophaga (Robineau-Desvoidy, 1830)(双翅目:Sarcophagidae)是一种常见的尸食性蝇类。在本研究中,我们提出了一种基于图像的深度学习框架来自动分类S. peregrina蛹内发育年龄,以提高PMI估计的准确性。在25°C下饲养蛹,收集不同发育阶段(第1天至第11天)的样品。去除蛹后,采集蛹内形态的高分辨率图像,构建数据集。首先使用ResNet50网络提取感兴趣的区域,然后使用Vision Transformer (ViT)模型对发育阶段进行端到端分类。该方法的分类精度为94.00 %,召回率为93.41 %,f1分数为93.43 %。这些研究结果表明,深度学习可以作为人工形态学评估的有效和客观的替代方法,减少对专家经验的依赖。该方法为基于昆虫证据的标准化、快速、准确的PMI推断建立了可行的人工智能辅助途径,为法医调查提供了实用价值。
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引用次数: 0
Forensic gender and stature identification from footprint images using machine learning 利用机器学习从脚印图像中识别法医性别和身材
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-12-02 DOI: 10.1016/j.forsciint.2025.112760
Mashal Khalid, Tatiana Kameneva, Chris McCarthy
The analysis of footprints to infer human characteristics and biometric information is a valuable tool in forensic investigation. Traditional methods rely primarily on physical measurements and observational analysis, which requires significant time, effort and specialized expert judgment. This study proposes a novel, automated, end-to-end approach to gender classification and stature estimation from footprints, using image analysis and traditional machine learning methods. Specifically, this we employ a image pre-processing techniques for Region of Interest extraction to segment foot prints in images and identify toe and heel exterior points through convexity and defectiveness points. The study utilized a dataset of 396 footprints from 33 participants (18 males and 15 females, aged 18–48 years, height range 148–182 cm). Hyper-parameter tuning via grid search optimization is employed and traditional Machine Learning (ML) models, including Logistic Regression (LR), Gaussian Naive Bayes (GNB), K-Nearest Neighbor (KNN), Decision Tree Classifier (DTC), and Support Vector Machine (SVM), are benchmarked for the task of both inferring gender, and stature. We specifically focus on traditional ML methods due to their relatively modest training data requirements, with the aim of establishing their feasibility for such forensic analysis. KNN demonstrated better accuracy overall for gender classification achieving 0.91 accuracy, while Extreme Gradient Boosting (XGBoost) outperformed other methods for stature estimation with MAE of 4.10 cm and RMSE of 5.42 cm, however varying strengths and weaknesses of each classifier for gender classification and stature estimation were observed. Our results suggest that the strongest performing traditional ML methods offer a feasible solution for such analysis, however expanding the training dataset to incorporate more footprint examples of more varying quality and depicting a greater diversity of population is likely necessary to fully realise a workable end-to-end solution. Such datasets may also open the door to more advanced deep learning methods.
通过脚印分析推断人类特征和生物特征信息是法医调查的重要工具。传统的方法主要依靠物理测量和观察分析,这需要大量的时间、精力和专业的专家判断。本研究提出了一种新颖的、自动化的端到端方法,利用图像分析和传统的机器学习方法从脚印中进行性别分类和身高估计。具体来说,我们采用图像预处理技术提取感兴趣区域来分割图像中的脚印,并通过凹凸点和缺陷点来识别脚趾和脚跟外部点。该研究使用了来自33名参与者(18名男性和15名女性,年龄在18 - 48岁,身高范围在148-182 cm)的396个足迹数据集。通过网格搜索优化进行超参数调优,并对传统的机器学习(ML)模型,包括逻辑回归(LR)、高斯朴素贝叶斯(GNB)、k近邻(KNN)、决策树分类器(DTC)和支持向量机(SVM)进行基准测试,以推断性别和身材。我们特别关注传统的机器学习方法,因为它们对训练数据的要求相对较低,目的是建立它们用于此类法医分析的可行性。总体而言,KNN在性别分类方面表现出更好的准确率,达到0.91,而Extreme Gradient Boosting (XGBoost)在身高估计方面表现优于其他方法,MAE为4.10 cm, RMSE为5.42 cm,但每种分类器在性别分类和身高估计方面都存在不同的优缺点。我们的结果表明,表现最好的传统机器学习方法为此类分析提供了可行的解决方案,然而,扩展训练数据集以纳入更多质量变化更大的足迹示例并描绘更大的人口多样性可能是完全实现可行的端到端解决方案所必需的。这样的数据集也可能为更先进的深度学习方法打开大门。
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引用次数: 0
Secular trends in femoral measurements and their implications for skeletal sex estimation in the Portuguese population 股骨测量的长期趋势及其对葡萄牙人口骨骼性别估计的影响。
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-12-01 DOI: 10.1016/j.forsciint.2025.112759
SiYang Zeng , Eugénia Cunha , Francisco Curate
This study investigates secular changes in femoral metric morphology within the Portuguese population. It aims to explore patterns of anthropometric data and assess how secular trends may influence the performance of univariable models for sex estimation. Using 449 skeletal samples (229 females and 220 males) from Portuguese individuals born between 1805 and 1947 and deceased between 1870 and 2012, six femoral measurements were analysed: vertical head diameter (FVHD), transverse head diameter (FTHD), neck height (FNH), neck axis length (FNAL), epicondylar breadth (FEB), and maximum length (FML). In the Portuguese population, significant correlations of FNH and FNAL with birth and death years were observed in both sexes, decreasing in FNH and increasing in FNAL. These findings suggest a secular trend toward a narrower and longer femoral neck. While FML increased over time in males, it remained relatively stable in females. Meanwhile, FVHD, FTHD, and FEB maintain a secular constancy in the Portuguese population. These findings underscore the need to consider temporal and biological influences when developing or applying forensic anthropological sex estimation models in a specific population. Additionally, this cross-sectional study found that both FNH and FML show statistically significant negative correlations with age at death. Further research using longitudinal data is needed to confirm whether these patterns result from degenerative processes, cohort effects, or both.
本研究调查了葡萄牙人口股骨计量形态的长期变化。它旨在探索人体测量数据的模式,并评估长期趋势如何影响性别估计的单变量模型的性能。从1805年至1947年出生、1870年至2012年死亡的葡萄牙人的449个骨骼样本(229名女性和220名男性)中,分析了6个股骨测量数据:垂直头直径(FVHD)、横向头直径(FTHD)、颈高(FNH)、颈轴长度(FNAL)、上髁宽度(FEB)和最大长度(FML)。在葡萄牙人口中,男女FNH和FNAL与出生和死亡年份显著相关,FNH下降,FNAL增加。这些结果表明股骨颈有变窄变长的长期趋势。虽然FML在男性中随着时间的推移而增加,但在女性中保持相对稳定。同时,FVHD、FTHD和FEB在葡萄牙人口中保持长期不变。这些发现强调了在特定人群中开发或应用法医人类学性别估计模型时考虑时间和生物学影响的必要性。此外,本横断面研究发现FNH和FML与死亡年龄呈统计学显著负相关。需要使用纵向数据的进一步研究来确认这些模式是由退行性过程、队列效应还是两者兼而有之。
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引用次数: 0
Towards harmonised practices in tracing staining inks from activated Intelligent Banknote Neutralisation Systems (IBNS): Findings from a multinational European survey 从激活的智能钞票中和系统(IBNS)追踪染色油墨的协调实践:来自欧洲多国调查的结果。
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-11-30 DOI: 10.1016/j.forsciint.2025.112757
Dimitrios Perisynakis, Christos Batis
This paper presents the findings from a multinational survey conducted in February 2024. Data from 18 forensic laboratories across 13 European countries were collected and analysed. The study investigates the implementation of IBNS (Intelligent Banknote Neutralisation Systems), the use of staining inks (indelible security inks usually containing forensic taggant agents) as a deterrent measure against physical attacks on ATMs (Automated Teller Machines) and CITs (Cash in Transit), and the traceability of ink-stained banknotes as well as other stained evidence. The analysis reveals the widespread reliance of the experts’ investigation on data supplied by companies, which is often not independently verifiable raising concerns about its exploitation for forensic conclusions. The paper emphasizes the need for (a) standardized procedures and oversight in IBNS supply chain, (b) improved law enforcement cooperation, and (c) centralised data frameworks. With this publication, we aim to formally document the survey's results as a legacy reference and establish a foundation for future collaboration between forensic laboratories, companies involved in the IBNS supply chain, law enforcement and regulatory authorities.
本文介绍了2024年2月进行的一项跨国调查的结果。来自13个欧洲国家的18个法医实验室的数据被收集和分析。该研究调查了IBNS(智能纸币中和系统)的实施,染色油墨(通常含有法医标记剂的不褪色安全油墨)的使用,作为对atm(自动柜员机)和cit(中途现金)的物理攻击的威慑措施,以及墨水污染的钞票以及其他污染证据的可追溯性。分析显示,专家们的调查普遍依赖于公司提供的数据,而这些数据往往无法独立验证,这引发了人们对利用这些数据得出法医结论的担忧。该文件强调需要(a) IBNS供应链中的标准化程序和监督,(b)改进执法合作,以及(c)集中数据框架。通过本出版物,我们的目标是正式记录调查结果,作为遗留参考,并为法医实验室、参与IBNS供应链的公司、执法部门和监管机构之间的未来合作奠定基础。
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引用次数: 0
Dorsal hand image comparison: A survey of image comparison practitioners 手背图像比较:对图像比较从业者的调查。
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-11-29 DOI: 10.1016/j.forsciint.2025.112758
Inga Siebke , Zuzana Obertová
Dorsal hand image comparison (DHIC) as a branch of forensic identification develops along with the rapid improvements in image resolution and thus dorsal hand features have become a viable area for morphological image comparisons. A short online survey targeting practitioners in image comparison and analysis was created to gain an overview of the global status quo of DHIC. In total, 32 valid responses from 18 different countries were received. Despite different levels of work experience of the participants, it seems that DHIC is increasingly used in a variety of case types. However, several limitations have been acknowledged, including the lack of training and best practice guidelines. In conclusion, DHIC is an emerging field in forensic investigation and practitioners call for structured training opportunities and the establishment of best practice guidelines. In addition, more research into various aspects of the dorsal hand features, such as the effect of ageing and kinship would be beneficial.
手背图像比对作为法医鉴定的一个分支,随着图像分辨率的快速提高而发展起来,手背特征已成为形态学图像比对的一个可行领域。针对图像比较和分析从业人员进行了一项简短的在线调查,以获得对DHIC全球现状的概述。总共收到了来自18个不同国家的32份有效答复。尽管参与者的工作经验水平不同,但DHIC似乎越来越多地用于各种病例类型。但是,也承认了一些限制,包括缺乏培训和最佳做法准则。总之,DHIC是法医调查的一个新兴领域,从业人员呼吁提供结构化的培训机会和建立最佳实践指南。此外,更多地研究手背特征的各个方面,如年龄和亲属关系的影响将是有益的。
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引用次数: 0
Technical note: Automated data extraction from autopsy reports using a custom Python script 技术说明:使用自定义Python脚本从尸检报告中自动提取数据
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-11-26 DOI: 10.1016/j.forsciint.2025.112756
Johannes Rødbro Busch, Carl Johan Wingren
Evidence-based forensic research necessitates the creation of large and valid datasets. However, in our experience many departments face a challenge in how to extract this data from electronically archived records. This technical note describes a custom script created in the Python programming language. The program can extract data on decedent sex, age, body height, body weight, organ weight, organ dimensions, degree of putrefaction, listed cause of death, medical history and scene description from approximately 23,000 records in under two hours. Validity for many of these data are around 97–99 %. The program can be modified to extract any type of information. Data that are structured uniformly in the records result in higher data validity. Compared with manual extraction of data, automated extraction provide several benefits, including speed, accuracy, and flexibility.
基于证据的法医研究需要创建大量有效的数据集。然而,根据我们的经验,许多部门都面临着如何从电子存档记录中提取这些数据的挑战。本技术说明描述了用Python编程语言创建的自定义脚本。该程序可以在两小时内从大约23000份记录中提取死者的性别、年龄、身高、体重、器官重量、器官尺寸、腐烂程度、列出的死亡原因、病史和现场描述等数据。这些数据的有效性在97-99 %左右。该程序可以被修改以提取任何类型的信息。记录中结构统一的数据可以提高数据的有效性。与手动提取数据相比,自动提取提供了几个优点,包括速度、准确性和灵活性。
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引用次数: 0
Proof-of-concept study on an integrated multiplex pyrosequencing system and CLPSQ-Net algorithm for multiplex microhaplotypes genotyping 多重焦磷酸测序系统和CLPSQ-Net算法用于多重微单倍型基因分型的概念验证研究
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-11-26 DOI: 10.1016/j.forsciint.2025.112751
Yueyan Cao , Qiang Zhu , Yuguo Huang , Yuhan Hu , Haoyu Wang , Yufang Wang , Ji Zhang
Microhaplotypes (MHs), characterized by short fragment lengths and high polymorphism, hold great promise for forensic applications. Here, we present a streamlined multiplex MHs detection system based on pyrosequencing (PSQ), incorporating several key innovations: (1) selection of highly polymorphic short-fragment MHs from the 1000 Genomes Project (1KGP); (2) algorithmic optimization of nucleotide dispensation orders for accurate haplotyping; (3) utilization of peak-height simulation to augment the dataset, overcoming the limitation of scarce empirical data for machine learning; and (4) CLPSQ-Net, a deep contrastive learning framework for deconvoluting multiplex PSQ signals. In this feasibility study, the system demonstrated simultaneous genotyping of four MH loci (6–7 haplotypes per locus) and an input sensitivity of 100 pg of DNA in a preliminary evaluation. Simulated and experimental signals showed high concordance (cosine similarity >0.98 for uniplex, >0.99 for multiplex). CLPSQ-Net achieved 89.7 % classification accuracy and an F1-score of 88.4 % on experimental data, outperforming traditional regression methods (which exhibited accuracies below 38 % on the PSQ-8 dataset) by a substantial margin. This proof-of-concept study establishes a scalable framework for multiplex MH genotyping via PSQ. Our method-development study offers substantial improvements over conventional workflows: superior base-calling accuracy, streamlined efficiency with automated dispensation ordering, and expanded utility for complex loci profiling.
微单倍型(Microhaplotypes, MHs)具有片段长度短、多态性高的特点,在司法鉴定中具有广阔的应用前景。在这里,我们提出了一种基于焦磷酸测序(PSQ)的流线型多重mhh检测系统,其中包括几个关键创新:(1)从1000基因组计划(1KGP)中选择高度多态性的短片段mhh;(2)精确单倍型的核苷酸分配顺序算法优化;(3)利用峰高模拟对数据集进行扩充,克服了机器学习经验数据稀缺的限制;(4) CLPSQ-Net,一个用于解卷积多重PSQ信号的深度对比学习框架。在这项可行性研究中,该系统在初步评估中显示了4个MH位点(每个位点6-7个单倍型)的同时基因分型和100 pg的DNA输入灵敏度。模拟信号和实验信号显示出高一致性(单路余弦相似度>;0.98,多路余弦相似度>;0.99)。CLPSQ-Net在实验数据上实现了89.7 %的分类准确率和88.4 %的f1分数,大大优于传统的回归方法(在PSQ-8数据集上显示的准确率低于38 %)。这项概念验证研究建立了一个可扩展的框架,通过PSQ进行多重MH基因分型。我们的方法开发研究提供了传统工作流程的实质性改进:优越的碱基调用准确性,自动化分配排序的流线型效率,以及复杂位点分析的扩展效用。
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引用次数: 0
Chemical profiling of cocaine using portable spectroscopic techniques: Towards timely illicit drug intelligence 使用便携式光谱技术对可卡因进行化学分析:以期及时获得非法药物情报
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-11-26 DOI: 10.1016/j.forsciint.2025.112753
Marina Charest, Susanna Meola, Laëtitia Gasté, Pierre Esseiva
Chemical profiling of illicit drugs plays a key role in linking seizures and supporting investigations related to illicit drug trafficking. Separative analytical techniques, such as gas chromatography-mass spectrometry (GC-MS), remain the standard method for chemical profiling thanks to their ability to provide detailed insights into chemical composition. However, valuable intelligence generated through this process often remains unexploited by investigators, primarily because the associated analytical and administrative procedures delay its availability. These delays can impact the early phase of investigations when rapid information is most critical. This study evaluates the feasibility of using rapid and portable spectroscopic techniques to initiate the illicit drug profiling process earlier in investigations. 277 cocaine specimens were profiled using the reference GC-MS method and classified into their respective chemical classes. These specimens were also analyzed with near-infrared (NIR) and Raman spectroscopy, and pairwise spectral comparisons using the Euclidean distance metric were performed between the populations of linked (intra-variability) and unlinked (inter-variability) samples. Results demonstrate that these techniques effectively discriminate cocaine specimens identified as either linked or unlinked by the reference method, with NIR spectroscopy showing higher discrimination. Finally, the practical implementation, added value and limitations of integrating these rapid techniques into the illicit drug profiling process are discussed.
非法药物的化学特征分析在联系缉获和支持与非法毒品贩运有关的调查方面发挥着关键作用。分离分析技术,如气相色谱-质谱(GC-MS),仍然是化学分析的标准方法,因为它们能够提供对化学成分的详细了解。然而,通过这一过程产生的宝贵情报往往未被调查人员利用,主要是因为相关的分析和行政程序延误了情报的提供。这些延误可能会影响调查的早期阶段,而快速的信息是最关键的。本研究评估了在调查早期使用快速和便携式光谱技术启动非法药物分析过程的可行性。采用气相色谱-质谱法对277份可卡因样品进行了分析,并将其分类。这些样品还使用近红外(NIR)和拉曼光谱进行了分析,并使用欧几里得距离度量对连接(内部变异性)和未连接(内部变异性)样品群体进行了两两光谱比较。结果表明,该方法能有效地鉴别参考方法鉴定的连接或未连接的可卡因样品,其中近红外光谱具有较高的鉴别能力。最后,讨论了将这些快速技术整合到非法药物分析过程中的实际实施、附加价值和局限性。
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引用次数: 0
Effectiveness of a washing protocol for the removal of gunshot residue from forensically relevant Lucilia sericata larvae 清洗方案去除与法医相关的丝光绿蝇幼虫枪弹残留物的有效性
IF 2.5 3区 医学 Q1 MEDICINE, LEGAL Pub Date : 2025-11-26 DOI: 10.1016/j.forsciint.2025.112752
Michaela A. Storen, Xavier A. Conlan, Damien L. Callahan, Michelle L. Harvey
Blowfly larvae (Diptera: Calliphoridae) have been suggested to have practical application as a toxicological target in forensic science. These larvae may be of use for gunshot residue analysis where traditional analytical targets such as the liver or entry wounds are absent and may allow an opportunity to identify toxins, drugs and gunshot residue from a corpse. A primary weakness of GSR identification in entomotoxicology is the lack of a standardised methodology for processing the larvae for accurate determination of GSR. In this study, Lucilia sericata (Meigen) larvae were exposed to pork mince that was shot 4 times at close range, the larvae were then sampled 12 hourly. A wash protocol for the larvae was developed and the concentrations of Ga, Ba, and Pb, key GSR markers were determined in Lucilia sericata larvae and the solution used to wash them to identify the effectiveness of the cleaning process. Both the whole larvae and each respective wash solution were analysed using inductively coupled plasma mass spectrometry (ICP-MS). Analysis of the wash solutions revealed that a minimum of two washes were required to remove external contaminants prior to ICP-MS analysis of the whole larvae. This work demonstrates the importance of implementing an effective wash protocol when measuring GSR of forensic interest within larvae, as contaminants on the surface of the larvae could lead to misinterpretation of data.
苍蝇幼虫(双翅目:蝇科)作为毒理学靶点在法医学中具有实际应用价值。在传统的分析目标如肝脏或射入伤口缺失的情况下,这些幼虫可用于枪弹残留物分析,并可能有机会从尸体上识别毒素、药物和枪弹残留物。昆虫毒理学鉴定GSR的一个主要弱点是缺乏标准化的方法来处理幼虫以准确测定GSR。本试验采用肉糜近距离射击4次,每隔12 h采集丝光绿蝇幼虫。研究了丝光绿蝇幼虫的清洗方案,测定了丝光绿蝇幼虫及其清洗液中关键GSR标记物Ga、Ba和Pb的浓度,以确定清洗过程的有效性。采用电感耦合等离子体质谱法(ICP-MS)对整个幼虫和各自的洗涤液进行分析。对洗涤液的分析表明,在对整个幼虫进行ICP-MS分析之前,至少需要两次洗涤以去除外部污染物。这项工作证明了在测量幼虫体内法医感兴趣的GSR时实施有效清洗方案的重要性,因为幼虫表面的污染物可能导致数据的误解。
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引用次数: 0
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Forensic science international
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