Pub Date : 2024-09-30DOI: 10.1016/j.forsciint.2024.112239
Jean-Alexandre Patteet, Christophe Champod
Toolmarks examination validity and subjectivity have come under scrutiny. This research focuses on the case of cutting plier marks. This paper presents an automatic comparison method and assesses its performance. It is designed to assign a weight to the forensic evidence (i.e, a comparison between toolmarks) with a likelihood ratio (LR). 3D topographies are acquired and treated to be compared using a set of correlation metrics. A machine learning algorithm combines comparison metrics and enables LR computation. Pliers of various brands and models were used to study the variability both within and between tools. We explained why the specific zone (area along the blade) has to be chosen to build the within-source variability and how the between-source variability can be built in different scenarios. Misleading evidence rates between 0 % and 4 % have been measured and it demonstrates the accuracy of the method when applied on the pliers used.
{"title":"Automated comparison and evaluation of striated cutting plier toolmarks on metal wires","authors":"Jean-Alexandre Patteet, Christophe Champod","doi":"10.1016/j.forsciint.2024.112239","DOIUrl":"10.1016/j.forsciint.2024.112239","url":null,"abstract":"<div><div>Toolmarks examination validity and subjectivity have come under scrutiny. This research focuses on the case of cutting plier marks. This paper presents an automatic comparison method and assesses its performance. It is designed to assign a weight to the forensic evidence (i.e, a comparison between toolmarks) with a likelihood ratio (LR). 3D topographies are acquired and treated to be compared using a set of correlation metrics. A machine learning algorithm combines comparison metrics and enables LR computation. Pliers of various brands and models were used to study the variability both within and between tools. We explained why the specific zone (area along the blade) has to be chosen to build the within-source variability and how the between-source variability can be built in different scenarios. Misleading evidence rates between 0 % and 4 % have been measured and it demonstrates the accuracy of the method when applied on the pliers used.</div></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112239"},"PeriodicalIF":2.2,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142371430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study is to examine the time-related changes of alveolar bone in 2D images quantitatively and to estimate age groups based on the change index.
Materials and methods
The 238 panoramic X-ray images and 140 CT panoramic reconstructed images of the permanent dentition period were used to examine age-related changes. Comparisons between the younger age group and each of the other age groups were calculated using the landmark method of Procrustes analysis. As aging changes were observed in each age group, age estimation was performed using antemortem panoramic X-ray images and postmortem CT images so that they could be used in practice. The CT images used in the age estimation were performed using forty-two postmortem CT panoramic reconstructed images of known age submitted to the judicial autopsy.
Results
Both panoramic and CT images showed changes in the alveolar bone over time. Age estimation using postmortem CT images provided a certain assessment.
Conclusion
In this study, clinically observed changes in alveolar bone over time were quantified on the images. Furthermore, the possibility of age estimation by alveolar bone was also suggested. The use of an updatable clinical database that can be stored in coordinate values offers the potential for age estimation in line with the times.
{"title":"Evaluation of age estimation using alveolar bone images","authors":"Hideko Fujimoto , Kaori Kimura-Kataoka , Akiko Takeuchi , Motoo Yoshimiya , Ryoji Kawakami","doi":"10.1016/j.forsciint.2024.112237","DOIUrl":"10.1016/j.forsciint.2024.112237","url":null,"abstract":"<div><h3>Objective</h3><div>The purpose of this study is to examine the time-related changes of alveolar bone in 2D images quantitatively and to estimate age groups based on the change index.</div></div><div><h3>Materials and methods</h3><div>The 238 panoramic X-ray images and 140 CT panoramic reconstructed images of the permanent dentition period were used to examine age-related changes. Comparisons between the younger age group and each of the other age groups were calculated using the landmark method of Procrustes analysis. As aging changes were observed in each age group, age estimation was performed using antemortem panoramic X-ray images and postmortem CT images so that they could be used in practice. The CT images used in the age estimation were performed using forty-two postmortem CT panoramic reconstructed images of known age submitted to the judicial autopsy.</div></div><div><h3>Results</h3><div>Both panoramic and CT images showed changes in the alveolar bone over time. Age estimation using postmortem CT images provided a certain assessment.</div></div><div><h3>Conclusion</h3><div>In this study, clinically observed changes in alveolar bone over time were quantified on the images. Furthermore, the possibility of age estimation by alveolar bone was also suggested. The use of an updatable clinical database that can be stored in coordinate values offers the potential for age estimation in line with the times.</div></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112237"},"PeriodicalIF":2.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142375372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.forsciint.2024.112236
Anna G. Golovkina , Oleg R. Karpukhin , Anastasia V. Kravchenko , Evgeniia M. Khairullina , Ilya I. Tumkin , Andrey V. Kalinichev
Fraudulent activities often involve document manipulation, which poses a significant challenge to forensic science. To address this issue, a novel method was developed that combines intended artificial UV pre-degradation, digital color analysis (DCA) of stroke images, and various machine learning (ML) models. This method can cluster blue ballpoint pen inks and predict their photodegradation time. The results of the study indicate that the k-shape clustering method is highly effective in differentiating between inks based on their degradation curve patterns and HSV or RBS color features, aligning well with results from chromatography analyses. Furthermore, the random forest regression model demonstrated superior performance in predicting age, exhibiting the highest coefficients of determination. The DCA-ML method is a straightforward, cost-effective, and highly accurate solution for clustering blue pen inks. Using photodegradation curves to predict document age could eliminate the need for conventional physicochemical analysis techniques.
{"title":"Digital color analysis and machine learning for ballpoint pen ink clustering and aging investigation","authors":"Anna G. Golovkina , Oleg R. Karpukhin , Anastasia V. Kravchenko , Evgeniia M. Khairullina , Ilya I. Tumkin , Andrey V. Kalinichev","doi":"10.1016/j.forsciint.2024.112236","DOIUrl":"10.1016/j.forsciint.2024.112236","url":null,"abstract":"<div><div>Fraudulent activities often involve document manipulation, which poses a significant challenge to forensic science. To address this issue, a novel method was developed that combines intended artificial UV pre-degradation, digital color analysis (DCA) of stroke images, and various machine learning (ML) models. This method can cluster blue ballpoint pen inks and predict their photodegradation time. The results of the study indicate that the k-shape clustering method is highly effective in differentiating between inks based on their degradation curve patterns and HSV or RBS color features, aligning well with results from chromatography analyses. Furthermore, the random forest regression model demonstrated superior performance in predicting age, exhibiting the highest coefficients of determination. The DCA-ML method is a straightforward, cost-effective, and highly accurate solution for clustering blue pen inks. Using photodegradation curves to predict document age could eliminate the need for conventional physicochemical analysis techniques.</div></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112236"},"PeriodicalIF":2.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-26DOI: 10.1016/j.forsciint.2024.112238
Nga Weng (Chloe) Ieong , Mickayla Dustin , Sally Coulson
Polymers are present in many different products, such as paints, plastics, and rubbers, which are routinely encountered in forensic casework. Comparison of such samples involves an initial visual examination followed by comparison of the chemical compositions of the exhibits. Techniques such as Fourier transform infrared spectroscopy (FTIR) and pyrolysis gas chromatography – mass spectrometry (PyGC-MS) have been reported for determining the chemical compositions of polymers in forensic samples. Double-shot pyrolysis gas chromatography – mass spectrometry (DS-PyGC-MS) is an extension of single-shot pyrolysis gas chromatography – mass spectrometry (SS-PyGC-MS) which is the current PyGC-MS method used in most forensic laboratories. DS-PyGC-MS involves a preliminary thermal desorption GC-MS step, followed by the pyrolysis GC-MS step, with this second step being analogous to SS-PyGC-MS. The pyrolyser furnace operates at a lower temperature during the thermal desorption step, allowing low volatility compounds, such as additives, to be thermally desorbed and detected, minimising interference from the polymeric component of the sample. This pilot study analysed four different polymeric substrates, commonly encountered in forensic casework, by DS-PyGC-MS. The substrates chosen were tyre rubber, road cones, cling film, and shotgun wads. The aim was to investigate whether more chemical information was generated by DS-PyGC-MS compared to SS-PyGC-MS, potentially providing increased discrimination of such samples. Qualitative results showed that tyre rubber and road cones were ideal substrates for DS-PyGC-MS. A wide range of additives were detected in these samples in the thermal desorption step, which were not detected using SS-PyGC-MS. All of the rubber tyres (n=5) and road cones (n=6) were able to be uniquely distinguished using DS-PyGC-MS. Some additional compounds were detected in the thermal desorption analysis of shotgun wads (n=4), providing increased discrimination compared to SS-PyGC-MS. For the cling film samples analysed (n=7) the polyethylene-based cling films (n=6) could not be distinguished from each other, with no compounds detected in the thermal desorption step. The other cling film sample contained a mixture of polylactic acid (PLA) and polybutylene adipate terephthalate (PBAT) and could easily be distinguished from the polyethylene-based cling films using either SS- or DS-PyGC-MS, or other common analytical methods such as Fourier transform infrared spectroscopy (FTIR). This pilot study has demonstrated that DS-PyGC-MS has the potential to provide more comprehensive chemical composition information for some polymeric substrates and is a promising method for the forensic comparison of polymer evidence.
{"title":"Analysis of forensic polymer samples using double shot pyrolysis gas chromatography – Mass spectrometry","authors":"Nga Weng (Chloe) Ieong , Mickayla Dustin , Sally Coulson","doi":"10.1016/j.forsciint.2024.112238","DOIUrl":"10.1016/j.forsciint.2024.112238","url":null,"abstract":"<div><div>Polymers are present in many different products, such as paints, plastics, and rubbers, which are routinely encountered in forensic casework. Comparison of such samples involves an initial visual examination followed by comparison of the chemical compositions of the exhibits. Techniques such as Fourier transform infrared spectroscopy (FTIR) and pyrolysis gas chromatography – mass spectrometry (PyGC-MS) have been reported for determining the chemical compositions of polymers in forensic samples. Double-shot pyrolysis gas chromatography – mass spectrometry (DS-PyGC-MS) is an extension of single-shot pyrolysis gas chromatography – mass spectrometry (SS-PyGC-MS) which is the current PyGC-MS method used in most forensic laboratories. DS-PyGC-MS involves a preliminary thermal desorption GC-MS step, followed by the pyrolysis GC-MS step, with this second step being analogous to SS-PyGC-MS. The pyrolyser furnace operates at a lower temperature during the thermal desorption step, allowing low volatility compounds, such as additives, to be thermally desorbed and detected, minimising interference from the polymeric component of the sample. This pilot study analysed four different polymeric substrates, commonly encountered in forensic casework, by DS-PyGC-MS. The substrates chosen were tyre rubber, road cones, cling film, and shotgun wads. The aim was to investigate whether more chemical information was generated by DS-PyGC-MS compared to SS-PyGC-MS, potentially providing increased discrimination of such samples. Qualitative results showed that tyre rubber and road cones were ideal substrates for DS-PyGC-MS. A wide range of additives were detected in these samples in the thermal desorption step, which were not detected using SS-PyGC-MS. All of the rubber tyres (n=5) and road cones (n=6) were able to be uniquely distinguished using DS-PyGC-MS. Some additional compounds were detected in the thermal desorption analysis of shotgun wads (n=4), providing increased discrimination compared to SS-PyGC-MS. For the cling film samples analysed (n=7) the polyethylene-based cling films (n=6) could not be distinguished from each other, with no compounds detected in the thermal desorption step. The other cling film sample contained a mixture of polylactic acid (PLA) and polybutylene adipate terephthalate (PBAT) and could easily be distinguished from the polyethylene-based cling films using either SS- or DS-PyGC-MS, or other common analytical methods such as Fourier transform infrared spectroscopy (FTIR). This pilot study has demonstrated that DS-PyGC-MS has the potential to provide more comprehensive chemical composition information for some polymeric substrates and is a promising method for the forensic comparison of polymer evidence.</div></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112238"},"PeriodicalIF":2.2,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-25DOI: 10.1016/j.forsciint.2024.112235
Andrea Palamenghi, Cristina Cattaneo
Migrant deaths have persistently intensified in the last 30 years, followed by inconsistent engagement of official policies to tackle the lack of identifications. Forensic anthropologists have been increasingly involved in the recovery, analysis and identification of human remains associated with migrants, and are therefore the most well-versed practitioners in raising awareness of policy makers about this plight. This review describes the current state-of-the-art of the forensic anthropological practice and research on unidentified migrants. The focus of the review is twofold: one section explores the current legal, logistical, and administrative issues related to management and identification activities, primarily in Europe and North America. The second section addresses the scientific contributions and new challenges that forensic anthropologists face when analyzing skeletal remains from underrepresented and unreferenced population groups. According to this literature review, forensic anthropologists are the professionals most actively involved in addressing the issue of unidentified migrants. Despite significant efforts, several challenges still hinder identifications in this context, including the inability to create comprehensive databases for collecting postmortem and antemortem data. Recently, scientific research in forensic anthropology has begun to address the limitations in developing reliable biological profiles for deceased migrants, as these groups are often inadequately represented in existing skeletal reference populations. Finally, this review highlights the global efforts of the scientific community to integrate these victims into a well-established identification system, aiming to foster public responsibility and promote the adoption of unified and innovative strategies to comprehensively address and resolve this social, scientific, and humanitarian crisis.
{"title":"The response of the forensic anthropology scientific community to migrant deaths: Where are we at and where do we stand?","authors":"Andrea Palamenghi, Cristina Cattaneo","doi":"10.1016/j.forsciint.2024.112235","DOIUrl":"10.1016/j.forsciint.2024.112235","url":null,"abstract":"<div><div>Migrant deaths have persistently intensified in the last 30 years, followed by inconsistent engagement of official policies to tackle the lack of identifications. Forensic anthropologists have been increasingly involved in the recovery, analysis and identification of human remains associated with migrants, and are therefore the most well-versed practitioners in raising awareness of policy makers about this plight. This review describes the current state-of-the-art of the forensic anthropological practice and research on unidentified migrants. The focus of the review is twofold: one section explores the current legal, logistical, and administrative issues related to management and identification activities, primarily in Europe and North America. The second section addresses the scientific contributions and new challenges that forensic anthropologists face when analyzing skeletal remains from underrepresented and unreferenced population groups. According to this literature review, forensic anthropologists are the professionals most actively involved in addressing the issue of unidentified migrants. Despite significant efforts, several challenges still hinder identifications in this context, including the inability to create comprehensive databases for collecting postmortem and antemortem data. Recently, scientific research in forensic anthropology has begun to address the limitations in developing reliable biological profiles for deceased migrants, as these groups are often inadequately represented in existing skeletal reference populations. Finally, this review highlights the global efforts of the scientific community to integrate these victims into a well-established identification system, aiming to foster public responsibility and promote the adoption of unified and innovative strategies to comprehensively address and resolve this social, scientific, and humanitarian crisis.</div></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112235"},"PeriodicalIF":2.2,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-20DOI: 10.1016/j.forsciint.2024.112233
Gabriel Ângelo da Silva Gomes , Lílian Pedrosa Marouelli de Oliveira , Daniel da Silva Carvalho , Felipe Carlos de Araújo Brito , Raul Yukihiro Matsushita
This study introduces a comprehensive inventory of 54 fingerprint minutiae and their variations aimed at standardizing characteristic point identification within forensic science. By analyzing a strategically sampled collection of fingerprints from the Brazilian Federal Police database, stratified by sex and geographic location, our research uncovers the complex interplay between various levels of fingerprint details (L1D, L2D, and L3D) and demographic factors such as sex and finger type. The sample encompassed the entire proposed list of minutiae, affirming the diversity and representativeness of the Brazilian populace, which had, for the first time, its minutiae frequency distributions studied. This investigation proposes a systematic approach for enhancing fingerprint identification accuracy by minimizing data categorization losses and lays the groundwork for more uniform comparative studies in the field. Our findings, derived from a review of contemporary studies and traditional identification manuals, suggest a step towards establishing a universally accepted standard for fingerprint minutiae classification.
{"title":"Standardizing fingerprint minutiae: A comprehensive inventory and statistical analysis based on Brazilian data","authors":"Gabriel Ângelo da Silva Gomes , Lílian Pedrosa Marouelli de Oliveira , Daniel da Silva Carvalho , Felipe Carlos de Araújo Brito , Raul Yukihiro Matsushita","doi":"10.1016/j.forsciint.2024.112233","DOIUrl":"10.1016/j.forsciint.2024.112233","url":null,"abstract":"<div><div>This study introduces a comprehensive inventory of 54 fingerprint minutiae and their variations aimed at standardizing characteristic point identification within forensic science. By analyzing a strategically sampled collection of fingerprints from the Brazilian Federal Police database, stratified by sex and geographic location, our research uncovers the complex interplay between various levels of fingerprint details (L1D, L2D, and L3D) and demographic factors such as sex and finger type. The sample encompassed the entire proposed list of minutiae, affirming the diversity and representativeness of the Brazilian populace, which had, for the first time, its minutiae frequency distributions studied. This investigation proposes a systematic approach for enhancing fingerprint identification accuracy by minimizing data categorization losses and lays the groundwork for more uniform comparative studies in the field. Our findings, derived from a review of contemporary studies and traditional identification manuals, suggest a step towards establishing a universally accepted standard for fingerprint minutiae classification.</div></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112233"},"PeriodicalIF":2.2,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-19DOI: 10.1016/j.forsciint.2024.112234
Felicia Bardan , Duncan Taylor , Julianne Henry
Operational forensic laboratories routinely perform immunological assays for detecting various body fluids. The ABAcard® p30 and HemaTrace® immunochromatographic tests from Abacus Diagnostics are used for detecting the p30 enzyme in human semen and human haemoglobin present in blood respectively. In early 2023, manufacturer modifications to the ABAcard® p30 and HemaTrace® tests resulted in a reduction in card size and volume of sample extract used in the recommended protocol. This change in card design and/or the reduced volume of sample extract may alter the sensitivity of the test. This study established and compared the limit of detection (LOD) for the old and newly modified ABAcard® p30 and HemaTrace® test cards. The LOD values showed that the new test cards were approximately 2.4-fold (HemaTrace® test) and 3.4-fold (p30 test) more sensitive than the old cards. Additionally, it was found that the new HemaTrace® test cards were more susceptible to the high dose hook effect. In response to the increased sensitivity, existing data pertaining to the reactivity of these test cards to non-target body fluid and substances warrants re-investigation to ensure positive results are interpreted correctly.
{"title":"Sensitivity assessment of the modified ABAcard® HemaTrace® and p30 immunochromatographic test cards","authors":"Felicia Bardan , Duncan Taylor , Julianne Henry","doi":"10.1016/j.forsciint.2024.112234","DOIUrl":"10.1016/j.forsciint.2024.112234","url":null,"abstract":"<div><p>Operational forensic laboratories routinely perform immunological assays for detecting various body fluids. The ABAcard® p30 and HemaTrace® immunochromatographic tests from Abacus Diagnostics are used for detecting the p30 enzyme in human semen and human haemoglobin present in blood respectively. In early 2023, manufacturer modifications to the ABAcard® p30 and HemaTrace® tests resulted in a reduction in card size and volume of sample extract used in the recommended protocol. This change in card design and/or the reduced volume of sample extract may alter the sensitivity of the test. This study established and compared the limit of detection (LOD) for the old and newly modified ABAcard® p30 and HemaTrace® test cards. The LOD values showed that the new test cards were approximately 2.4-fold (HemaTrace® test) and 3.4-fold (p30 test) more sensitive than the old cards. Additionally, it was found that the new HemaTrace® test cards were more susceptible to the high dose hook effect. In response to the increased sensitivity, existing data pertaining to the reactivity of these test cards to non-target body fluid and substances warrants re-investigation to ensure positive results are interpreted correctly.</p></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112234"},"PeriodicalIF":2.2,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-16DOI: 10.1016/j.forsciint.2024.112232
Juan He , Wenlong Liu , Xinxin Ren , Ding Ding , Lingfang He , Yunfeng Zhang , Bo Qiu
Understanding the factors that influence nitrite degradation in whole blood and developing methods for its stable preservation are crucial for ensuring accurate and reliable forensic identification in cases of nitrite poisoning. This study systematically monitored nitrite degradation and changes in hemoglobin proportions across different initial nitrite concentrations and blood samples. It was revealed that high nitrite concentrations rapidly reduced deoxyhemoglobin levels within the first 15 minutes and subsequently reacted with oxyhemoglobin at a slower rate. Therefore, the proportions of these two hemoglobin forms are key factors in determining nitrite degradation rates. Regarding preservation, the study examined the effects of low temperatures (4°C and −20°C) and various preservatives (potassium ferricyanide, N-ethylmaleimide) on nitrite stability. The results indicate that adding 6.6 g/L potassium ferricyanide can rapidly eliminate all deoxyhemoglobin and reduce oxyhemoglobin proportions to below 60 %, enabling stable preservation of high nitrite concentrations in whole blood for over 30 days at −20°C. The efficacy of potassium ferricyanide was further validated in forensic-acquired postmortem heart blood samples.
{"title":"Degradation and preservation of nitrites in whole blood","authors":"Juan He , Wenlong Liu , Xinxin Ren , Ding Ding , Lingfang He , Yunfeng Zhang , Bo Qiu","doi":"10.1016/j.forsciint.2024.112232","DOIUrl":"10.1016/j.forsciint.2024.112232","url":null,"abstract":"<div><p>Understanding the factors that influence nitrite degradation in whole blood and developing methods for its stable preservation are crucial for ensuring accurate and reliable forensic identification in cases of nitrite poisoning. This study systematically monitored nitrite degradation and changes in hemoglobin proportions across different initial nitrite concentrations and blood samples. It was revealed that high nitrite concentrations rapidly reduced deoxyhemoglobin levels within the first 15 minutes and subsequently reacted with oxyhemoglobin at a slower rate. Therefore, the proportions of these two hemoglobin forms are key factors in determining nitrite degradation rates. Regarding preservation, the study examined the effects of low temperatures (4°C and −20°C) and various preservatives (potassium ferricyanide, N-ethylmaleimide) on nitrite stability. The results indicate that adding 6.6 g/L potassium ferricyanide can rapidly eliminate all deoxyhemoglobin and reduce oxyhemoglobin proportions to below 60 %, enabling stable preservation of high nitrite concentrations in whole blood for over 30 days at −20°C. The efficacy of potassium ferricyanide was further validated in forensic-acquired postmortem heart blood samples.</p></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112232"},"PeriodicalIF":2.2,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-14DOI: 10.1016/j.forsciint.2024.112226
Julian Bickel , Alexander Müller , Hilke Jungen , Anne Szewczyk , Jörg Teske , Uta Küpper , Hilke Andresen-Streichert , Benjamin Ondruschka , Stefanie Iwersen-Bergmann
Drug-related fatalities in the EU are predominantly associated with opioids. MDMA (Ecstasy) consumption results in fewer lethal intoxications despite its widespread use. This study investigates MDMA-related fatalities, focusing on enantiomer ratios of MDMA and its metabolite MDA to explore the role of metabolism in fatal outcomes. MDMA induces euphoria, increased empathy, and physiological effects such as tachycardia, hypertension, and hyperthermia. Metabolism mainly involves CYP1A2 and CYP2D6, with polymorphism of the latter influencing metabolism rates. Our institute observed several MDMA-related fatalities, which prompted an investigation into the potential role of inefficient drug metabolism in these cases. A novel quantitative chiral analysis method was developed and validated for MDMA, MDA, amphetamine and methamphetamine enantiomers in human blood. Analysis of post mortem blood samples from eleven MDMA-related fatalities exhibited a wide range of concentrations and enantiomer ratios. Variability in R/S MDMA ratios, however, could be linked to the time period of metabolism. Hair analysis revealed high MDMA concentrations in all segments, irrespective of prior drug abuse anamnesis. Therefore, hair analysis may not be suitable for the assessment of past drug use in ecstasy-related fatalities. The results indicated that elevated levels of the MDMA enantiomer are correlated with longer survival times in cases of intoxication. However, there was no clear evidence for slowed MDMA metabolism as a cause of lethal intoxications. While challenges remain due to the diversity of cases, this study contributes valuable insights into ecstasy intoxications, aiding future interpretation of post mortem analysis.
{"title":"Post mortem chiral analysis of MDMA and MDA in human blood and hair","authors":"Julian Bickel , Alexander Müller , Hilke Jungen , Anne Szewczyk , Jörg Teske , Uta Küpper , Hilke Andresen-Streichert , Benjamin Ondruschka , Stefanie Iwersen-Bergmann","doi":"10.1016/j.forsciint.2024.112226","DOIUrl":"10.1016/j.forsciint.2024.112226","url":null,"abstract":"<div><p>Drug-related fatalities in the EU are predominantly associated with opioids. MDMA (Ecstasy) consumption results in fewer lethal intoxications despite its widespread use. This study investigates MDMA-related fatalities, focusing on enantiomer ratios of MDMA and its metabolite MDA to explore the role of metabolism in fatal outcomes. MDMA induces euphoria, increased empathy, and physiological effects such as tachycardia, hypertension, and hyperthermia. Metabolism mainly involves CYP1A2 and CYP2D6, with polymorphism of the latter influencing metabolism rates. Our institute observed several MDMA-related fatalities, which prompted an investigation into the potential role of inefficient drug metabolism in these cases. A novel quantitative chiral analysis method was developed and validated for MDMA, MDA, amphetamine and methamphetamine enantiomers in human blood. Analysis of post mortem blood samples from eleven MDMA-related fatalities exhibited a wide range of concentrations and enantiomer ratios. Variability in <em>R</em>/<em>S</em> MDMA ratios, however, could be linked to the time period of metabolism. Hair analysis revealed high MDMA concentrations in all segments, irrespective of prior drug abuse anamnesis. Therefore, hair analysis may not be suitable for the assessment of past drug use in ecstasy-related fatalities. The results indicated that elevated levels of the MDMA enantiomer are correlated with longer survival times in cases of intoxication. However, there was no clear evidence for slowed MDMA metabolism as a cause of lethal intoxications. While challenges remain due to the diversity of cases, this study contributes valuable insights into ecstasy intoxications, aiding future interpretation of post mortem analysis.</p></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112226"},"PeriodicalIF":2.2,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0379073824003074/pdfft?md5=011e3035cdd1fda27db567d04ba30472&pid=1-s2.0-S0379073824003074-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-14DOI: 10.1016/j.forsciint.2024.112218
Lauren Wilson , Paul Roffey , Kevin Lothridge , Sen Sgt Andy Rowan , Frank Crispino
The wars in Iraq and Afghanistan required that forensic science was used beyond the traditional law enforcement and criminal justice goals and applied to military operations. The 9/11 terror attacks in the United States (US) inspired further attacks in the Western World and highlighted the importance of national and international intelligence sharing for counterterrorism operations. Following the 9/11 attacks, anthrax was disseminated in the US mail system, demonstrating a successful modern use of biological agents. As a result, nations enhanced their Chemical, Biological, Radiological and Nuclear (CBRN) capabilities to prevent, prepare, respond, and recover from CBRN attacks but in the last decade, there has been a decrease in capabilities across military forensic science, counterterrorism, and CBRN. Recent changes in the global and strategic environment such as, the war in the Ukraine, the threat of grey zone conflicts (below the threshold of war) such as the conflict in the Middle East, and strategic competition, indicates international, national, state and local CBRN capabilities need to be reinvigorated in a world under digital transformations. There is a need for a harmonized approach to enhance the integration of military, law enforcement, and CBRN operations to support decision-making. In this paper, we look back to where we have come from and where we are now to make recommendations on where to from here for military forensic science and counterterrorism, including CBRN which intersects both of these systems.
{"title":"Intersection of CBRN, military forensic science, and counterterrorism","authors":"Lauren Wilson , Paul Roffey , Kevin Lothridge , Sen Sgt Andy Rowan , Frank Crispino","doi":"10.1016/j.forsciint.2024.112218","DOIUrl":"10.1016/j.forsciint.2024.112218","url":null,"abstract":"<div><div>The wars in Iraq and Afghanistan required that forensic science was used beyond the traditional law enforcement and criminal justice goals and applied to military operations. The 9/11 terror attacks in the United States (US) inspired further attacks in the Western World and highlighted the importance of national and international intelligence sharing for counterterrorism operations. Following the 9/11 attacks, anthrax was disseminated in the US mail system, demonstrating a successful modern use of biological agents. As a result, nations enhanced their Chemical, Biological, Radiological and Nuclear (CBRN) capabilities to prevent, prepare, respond, and recover from CBRN attacks but in the last decade, there has been a decrease in capabilities across military forensic science, counterterrorism, and CBRN. Recent changes in the global and strategic environment such as, the war in the Ukraine, the threat of grey zone conflicts (below the threshold of war) such as the conflict in the Middle East, and strategic competition, indicates international, national, state and local CBRN capabilities need to be reinvigorated in a world under digital transformations. There is a need for a harmonized approach to enhance the integration of military, law enforcement, and CBRN operations to support decision-making. In this paper, we look back to where we have come from and where we are now to make recommendations on where to from here for military forensic science and counterterrorism, including CBRN which intersects both of these systems.</div></div>","PeriodicalId":12341,"journal":{"name":"Forensic science international","volume":"364 ","pages":"Article 112218"},"PeriodicalIF":2.2,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号