Food Hydrocolloids for Health最新文献_第7页

Growth profile of Bacteroidetes strains under low molecular weight alginate fractions produced by non-thermal processing techniques 非热处理工艺生产的低分子量海藻酸盐馏分下拟杆菌门菌株的生长特征

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-02-05 DOI: 10.1016/j.fhfh.2025.100204

Pedro Rivero-Ramos , Pedro J. Fernandez-Julia , Dolores Rodrigo , María Benlloch-Tinoco , Jose Munoz-Munoz

Alginate is a dietary polysaccharide that is known to support the growth of particular gut-associated bacteria, including members of the Bacteroides genus. However, there is limited understanding of how key physicochemical characteristics of partially depolymerised alginate fractions affect the growth of various Bacteroidetes strains. In this study, the growth profile of Bacteroides thetaiotaomicron VPI-5482, Bacteroides cellulosilyticus WH2 and Dysgonomonas mossii DSMZ22836 on alginate fractions (M_w = 17–58 kDa) with different mannuronic and guluronic ratios (M/G ratio = 0.27–1.14) depolymerised by high hydrostatic pressures (HHP), pulsed electric fields (PEF) and H₂O₂-based treatments was screened. The tested strains were able to grow in the HHP, PEF and H₂O₂ treated samples. Growth of Bacteroides sp. was significantly affected by the physicochemical properties of the alginate substrates. The lower the M_w of the samples the faster the growth for the three strains investigated (p < 0.05, r = −0.7757). Higher M/G ratios significantly enhanced the growth of B. thetaiotaomicron VPI-5482 (p < 0.05, r = 0.9685). Finally, D. mossii DSMZ22836 was found to employ three enzymes (Aly, GH88, GH92) to degrade the low M_w alginate fractions. This study advances the understanding of strain-specific interactions of three glycan degraders in the presence of various low M_w alginate fractions.

藻酸盐是一种膳食多糖，已知可支持特定肠道相关细菌的生长，包括拟杆菌属的成员。然而，对于部分解聚海藻酸盐组分的关键物理化学特性如何影响各种拟杆菌门菌株的生长，人们的理解有限。本研究筛选了拟杆菌属（Bacteroides thetaiotaomicron VPI-5482）、拟杆菌属（Bacteroides cellulosilyticus WH2）和mossii Dysgonomonas DSMZ22836在高静水压力（HHP）、脉冲电场（PEF）和过氧化氢（h2o2）解聚的海藻酸盐组分（Mw = 17-58 kDa）上的生长情况。所检测的菌株能够在HHP、PEF和H2O2处理的样品中生长。海藻酸盐底物的理化性质对拟杆菌的生长有显著影响。样品的Mw越低，三种菌株的生长速度越快(p <；0.05, r = - 0.7757)。较高的M/G比显著促进了b.s thetaiotaomicron VPI-5482的生长(p <；0.05, r = 0.9685)。最后，发现D. mossii DSMZ22836利用Aly、GH88、GH92三种酶降解低Mw海藻酸盐馏分。本研究提高了对三种聚糖降解物在不同低分子量海藻酸盐组分存在下的菌株特异性相互作用的理解。

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引用次数: 0

Lipophilic bioactive compounds nano-micro encapsulation via coaxial electrohydrodynamic atomization: A review 亲脂性生物活性化合物的同轴电流体雾化纳米微胶囊化研究进展

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-27 DOI: 10.1016/j.fhfh.2025.100202

Elnaz Z. Fallahasghari, Ioannis S. Chronakis, Ana C. Mendes

Natural lipophilic bioactive compounds, which are essential for various physiological functions, present challenges in processing and formulation due to their physicochemical properties. Encapsulation techniques are utilized to improve their stability, solubility, and bioavailability. Electrohydrodynamic (EDH) processes, such as electrospinning and electrospraying, provide scalable methods for crafting nano-microfibers and capsules, making them ideal for encapsulating bioactive lipophilic compounds. Coaxial electrohydrodynamic processes generate core-shell structures with enhanced encapsulation performance, utilizing a broad range of polymers. This review discusses the research conducted over the past seven years on the core-shell electrosprayed capsules and electrospun fibers, for encapsulating natural lipophilic bioactive compounds. The aim is to promote the adoption of coaxial electrohydrodynamics as a straightforward and more sustainable encapsulation technology for lipophilic compounds.

天然亲脂性生物活性化合物具有多种生理功能，但由于其物理化学性质，在加工和配方方面存在挑战。包封技术用于提高其稳定性、溶解度和生物利用度。电流体动力学（EDH）工艺，如静电纺丝和静电喷涂，为制作纳米微纤维和胶囊提供了可扩展的方法，使它们成为包裹生物活性亲脂化合物的理想选择。同轴电流体动力学过程产生具有增强封装性能的核壳结构，利用广泛的聚合物。本文综述了近七年来在电喷雾胶囊和电纺丝纤维包封天然亲脂性生物活性化合物方面的研究进展。目的是促进采用同轴电流体动力学作为亲脂化合物的直接和更可持续的封装技术。

引用次数: 0

Influence of the use of gum Arabic or OSA starch and the drying process on the quality of the fava bean (Vicia faba) pod flour 阿拉伯胶或OSA淀粉的使用及干燥工艺对蚕豆豆荚粉品质的影响

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-26 DOI: 10.1016/j.fhfh.2025.100201

María del Mar Camacho, Narcisa Fernández-Vargas, Eva García-Martínez, Nuria Martínez-Navarrete

In the search for new foods that allow for healthy and sustainable eating, this study focuses on obtaining a flour from fava bean pods for consumption as a food ingredient. The influence of the drying process applied, hot air drying (AD) or freeze-drying (FD), and the incorporation of gum Arabic (GA) or sodium octenyl succinate starch (OSA) as potential carriers and encapsulant agents, on the composition, antioxidant activity and on some techno-functional properties of the powder was compared. In addition to the high starch and fiber content of the fava bean pod, GA increases the fiber soluble fraction, while OSA did so with starch. GA also provides minerals. On the other hand, the solid rather than viscous character of the films GA forms on the surface of the particles, unlike OSA, contributes to stabilize the compounds inside. FD keeps the product at a lower temperature during drying and involves obtaining a more porous structure that AD. However, the high phenol content of the bean pod, much higher than that of vitamin C and chlorophyll (mean values 40.8, 1.8 and 0.7 mg/g db, respectively), and the significant interaction between the hydrocolloids and drying process allows us to recommend the use of GA and AD to obtain a more economical powdered ingredient, healthier, and of the highest quality. These flours, in addition to having the highest phenol content and the highest antioxidant capacity, have the largest particle size and lowest porosity, which improves fluidity, hygroscopicity, wettability and solubility.

在寻找允许健康和可持续饮食的新食物的过程中，这项研究的重点是从蚕豆荚中提取面粉作为一种食品成分。比较了热风干燥（AD）和冷冻干燥（FD）、阿拉伯胶（GA）和琥珀酸辛烯基淀粉钠（OSA）作为潜在的载体和包封剂对粉末组成、抗氧化活性和部分工艺功能性能的影响。除了蚕豆豆荚的淀粉和纤维含量较高外，GA还增加了纤维可溶性部分，而OSA则增加了淀粉的可溶性部分。GA还提供矿物质。另一方面，与OSA不同，GA在颗粒表面形成的薄膜的固体特性而不是粘性特性有助于稳定内部的化合物。FD在干燥过程中使产品保持在较低的温度，并涉及获得比AD更多孔的结构。然而，豆荚的高酚含量，远高于维生素C和叶绿素（平均值分别为40.8,1.8和0.7 mg/g db），以及水胶体和干燥过程之间的显著相互作用，使我们推荐使用GA和AD来获得更经济，更健康和最高质量的粉状成分。这些面粉除了具有最高的酚含量和最高的抗氧化能力外，还具有最大的粒径和最低的孔隙率，从而提高了流动性、吸湿性、润湿性和溶解度。

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引用次数: 0

Alginate-based encapsulation of porcine placenta extract: Preparation, enteric sustained release, biological activities, and stability 藻酸盐包封猪胎盘提取物：制备、肠内缓释、生物活性和稳定性

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-24 DOI: 10.1016/j.fhfh.2024.100194

Anukul Taweechaipaisankul , Nutthanit Thumrongsiri , Walailuk Chonniyom , Paweena Dana , Prattana Tanyapanyachon , Monthira Rattanatayarom , Wannapa Chinchoosak , Nattika Saengkrit

The porcine placenta is a source of various advantageous bioactive molecules, however, the unsatisfactory appearance, together with its unsavory taste and odor, is still limiting its use as an ingredient in food products. Microencapsulation represents a promising technique to increase the use of valuable biowaste, as well as to promote its bioavailability and stability. Here, alginate microbeads containing porcine placenta extracts (pPEs) were developed by the ionotropic gelation method under various conditions, and many of their properties were tested. We found that placenta tissues prepared by freeze-drying could provide a great yield of protein derivatives and possessed 50.37 % anti-oxidative activity. Formulation 2 (F2) of alginate microbeads (80 % solution containing 2 % sodium alginate with 1 % bentonite, 20 % pPE formed in 0.5 % chitosan added into 1 % CaCl₂ solution) was chosen as the optimal encapsulated condition of pPEs. Generally, the F2 microbeads contained 57.5 µg/mL of pPE with 99.72 % entrapment efficiency. The average size measured by Mastersizer was 14.65 ± 0.08 µm. The release of pPEs under simulated gastrointestinal tract conditions at pH 2.0–6.8 was delayed at 2 h (38.7 %) compared with free pPE. The F2 microbeads showed positive biological effects of anti-oxidation. Moreover, the anti-inflammation effect was monitored via the reduction in the levels of cytokines, including IL-6, IL-8, and TNF-α. The F2 microbeads maintained their protein quantities for >120 days at 25 °C. Taken together, microbead fabrication, especially F2, is the optimal formula for pPEs, showing the potential to be applied as a prospective carrier of pPEs for oral administration.

猪胎盘是多种有利生物活性分子的来源，然而，其令人不满意的外观，连同其令人讨厌的味道和气味，仍然限制了其作为食品原料的使用。微胶囊化是一种很有前途的技术，可以增加有价值生物废物的利用，并提高其生物利用度和稳定性。本文采用亲离子凝胶法制备了含猪胎盘提取物（pPEs）的海藻酸盐微球，并对其性能进行了测试。冻干法制备的胎盘组织具有较高的蛋白衍生物产量和50.37%的抗氧化活性。选择配方2 （F2）为海藻酸钠微球的最佳包封条件（80%的溶液中含有2%的海藻酸钠和1%的膨润土，20%的pPE在0.5%的壳聚糖中加入1%的CaCl2溶液中形成）。F2微珠的pPE含量为57.5µg/mL，包封率为99.72%。Mastersizer测量的平均粒径为14.65±0.08µm。与游离pPE相比，在pH 2.0-6.8的模拟胃肠道条件下，pPE的释放延迟了2 h（38.7%）。F2微珠具有良好的抗氧化生物学效应。此外，通过降低细胞因子水平（包括IL-6、IL-8和TNF-α）来监测抗炎作用。F2微珠在25℃下保持蛋白量120天。综上所述，微珠制造，特别是F2，是ppe的最佳配方，显示出作为口服给药ppe的潜在载体的潜力。

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引用次数: 0

Comparison of different models for analyzing starch dynamic hydrolysis 分析淀粉动态水解的不同模型的比较

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-22 DOI: 10.1016/j.fhfh.2025.100200

Yuzhi Han , Cunxu Wei

Dynamic hydrolysis is an important property of starch, and hydrolysis parameters can provide information on starch qualities and applications in food and nonfood industries. The modified Michaelis-Menten equation (MME), single first-order kinetics equation (SKE), log of slope linear equation (LOSLE), or combination of parallel and sequential first-order kinetics equation (CPSKE) models are usually used to fit the dynamic hydrolysis data. In this study, the hydrolysis profiles of five starches were fitted by the MME, SKE, LOSLE and CPSKE models. The fits of the different models were evaluated using the sum of squares of residuals (SUMSQ), the fitting determination coefficient (R²), and the differences between the experimental and fitted data. When tested on the five starches, CPSKE model exhibited the best fit, LOSLE model had a better fit than did MME model, and SKE model had the poorest fit among them. Although these models had significantly different fitting qualities, the maximum extent of hydrolysis predicted by the different models was significantly positively correlated. The hydrolysis rate coefficient k fitted by the SKE model was significantly positively correlated with the k₁ fitted by the LOSLE and CPSKE models, but had no significant correlation with k₂ during phase 2 as fitted by the LOSLE and CPSKE models. The k₁ and k₂ values fitted by the LOSLE model were significantly positively correlated with the k₁ and k₂ parameters estimated by CPSKE model, respectively. This study could provide useful information for choosing fitting models for analyzing starch hydrolysis profiles.

动态水解是淀粉的重要特性，水解参数可以为淀粉的品质及其在食品和非食品工业中的应用提供信息。通常采用改进的Michaelis-Menten方程（MME）、单一阶动力学方程（SKE）、对数斜率线性方程（LOSLE）或并联和顺序一阶动力学方程（CPSKE）相结合的模型来拟合动态水解数据。采用MME、SKE、LOSLE和CPSKE模型拟合了5种淀粉的水解谱。采用残差平方和（SUMSQ）、拟合决定系数（R2）以及实验数据与拟合数据之间的差异来评估不同模型的拟合性。在5种淀粉的拟合检验中，CPSKE模型拟合最佳，LOSLE模型拟合优于MME模型，SKE模型拟合最差。虽然这些模型的拟合质量有显著差异，但不同模型预测的最大水解程度呈显著正相关。SKE模型拟合的水解速率系数k与LOSLE和CPSKE模型拟合的k1呈显著正相关，与LOSLE和CPSKE模型拟合的k2无显著相关。LOSLE模型拟合的k1和k2值与CPSKE模型估算的k1和k2参数分别呈显著正相关。本研究可为淀粉水解谱的拟合模型的选择提供参考。

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引用次数: 0

Date pomace polysaccharides-capped selenium nanoparticles: Biosynthesis, optimization, physicochemical characterization, biological activities, stability and gut microbiota modulation 枣渣多糖包封硒纳米粒子：生物合成、优化、理化表征、生物活性、稳定性和肠道菌群调节

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-20 DOI: 10.1016/j.fhfh.2025.100198

Gafar Babatunde Bamigbade , Athira Subhash , Mohammed Abdin , Hebah Jarusheh , Basim Abu-Jdayil , Shao-Quan Liu , Giovanni Palmisano , Abdelmoneim Ali , Afaf Kamal Eldin , Mutamed Ayyash

Selenium nanoparticles (Se-NP_S) are recognized for their bioavailability and low toxicity, though their inherent instability limits broader applications. This study explored the biosynthesis and functional properties of selenium nanoparticles (Se-NPS) stabilized with date pomace polysaccharides (MPS-NP_S). The MPS-NP_S characteristics, digestibility and impact on human gut microbiota were investigated. The MPS-NP_S were well-stabilized, spherical particles averaging 65.4 nm in size, with PDI (0.032), zeta potential (–21.07 mV) and notable antioxidant activity (82.7 % DPPH, 68.9 % ABTS, 491.0 µg/mL FRAP, and 961.2 µg/mL TAC at 100 mg/L). They also showed concentration-dependent enzyme inhibition: 86.8 % α-amylase, 53.9 % α-glucosidase, and 42.4 % ACE inhibition at 100 mg/L, along with anticancer effects against Caco-2 (32.3 %) and MCF-7 (11.3 %) at 50 mg/L. MPS-NP_S demonstrated broad antimicrobial activity against E. coli O 157:H7 (84.2 %), S. Typhimurium (82.5 %), S. aureus (77.2 %), and L. monocytogenes (89.24 %) and supported probiotic growth, indicating prebiotic potential. During fecal fermentation, they boosted short-chain fatty acid (SCFA) production and the abundance of SCFA-producing bacteria, including Gemmiger formicilis and Bifidobacterium species. These findings suggest MPS-NP_S as a promising functional ingredient for gut health and dietary supplementation.

硒纳米粒子（Se-NPS）因其生物利用度和低毒性而被公认，但其固有的不稳定性限制了其更广泛的应用。研究了枣渣多糖稳定纳米硒（Se-NPS）的生物合成及其功能特性。研究了MPS-NPS的特性、消化率及其对人体肠道菌群的影响。MPS-NPS稳定，平均粒径为65.4 nm，具有PDI (0.032)， zeta电位（-21.07 mV）和显著的抗氧化活性（在100 mg/L时，DPPH为82.7%，ABTS为68.9%，FRAP为491.0µg/mL， TAC为961.2µg/mL）。它们还表现出浓度依赖性的酶抑制作用：100 mg/L时α-淀粉酶抑制86.8%，α-葡萄糖苷酶抑制53.9%，ACE抑制42.4%，同时对Caco-2（32.3%）和MCF-7（11.3%）具有抗癌作用。MPS-NPS对大肠杆菌o157:H7（84.2%）、鼠伤寒沙门氏菌（82.5%）、金黄色葡萄球菌（77.2%）和单核增生乳杆菌（89.24%）具有广泛的抑菌活性，并支持益生菌的生长，具有益生元潜力。在粪便发酵过程中，他们促进了短链脂肪酸（SCFA）的产生和产生SCFA的细菌的丰度，包括双歧杆菌和双歧杆菌。这些发现表明，MPS-NPS是一种很有前景的肠道健康和膳食补充功能成分。

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引用次数: 0

Improving the techno-functionality of wild almond protein isolate-based films by its hydrolysates and cold plasma treatment 利用野生杏仁分离蛋白水解物和冷等离子体处理提高其技术功能性

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-20 DOI: 10.1016/j.fhfh.2025.100199

Zahra Tahsiri , Sara Hedayati , Mehrdad Niakousari

In the present study, composite films (COFI) using wild almond protein isolate (WAPI) and Persian gum (PG) in a ratio of 9:1 and varying amounts (0, 0.15%, 0.30%, and 0.60% (W/V)) of wild almond protein hydrolysate (PH) were developed. The COFI was exposed to atmospheric cold plasma (CP). The physicochemical, antioxidant, and antimicrobial characteristics of COFI before and after CP exposure were evaluated. The inclusion of PH did not significantly influence the COFI's moisture content biodegradability; however, the film thickness and film water vapor permeability increased. As the amount of PH in the COFI rose from 0 to 0.60%, the total phenolic content as well as antioxidant activity (determined by the DPPH and ABTS assays) were enhanced. While not being effective against Escherichia coli, the COFI containing PH exhibited inhibitory effects against Staphylococcus aureus. CP treatment improved the COFI in terms of their mechanical attributes without compromising antimicrobial and antioxidant features. Analysis of the FTIR spectra showed detectable enhancements in peak intensity at 1400, 1538, and 1628 cm⁻¹, while micrograph patterns displayed an increase in the coarseness of COFIs, following cold plasma treatment. Overall, the COFIs with 0.60% PH (W/V) treated with cold plasma were the most functionally favorable.

采用野生杏仁分离蛋白（WAPI）和波斯胶（PG）以9:1的比例和不同PH（0、0.15%、0.30%和0.60% (W/V)）的野生杏仁水解蛋白（PH）制备复合膜（COFI）。COFI暴露于大气冷等离子体（CP）中。评估了CP暴露前后COFI的理化、抗氧化和抗菌特性。PH包合对COFI的含水率、生物降解性无显著影响；膜厚和膜的水蒸气渗透性增加。随着COFI中PH值从0增加到0.60%，总酚含量和抗氧化活性（通过DPPH和ABTS测定）均增强。虽然对大肠杆菌无效，但含有PH的COFI对金黄色葡萄球菌有抑制作用。CP处理在不影响抗菌和抗氧化特性的情况下提高了COFI的力学性能。FTIR光谱分析显示，在1400、1538和1628 cm−1处的峰值强度可检测到增强，而显微图像显示，冷等离子体处理后，cofi的粗糙度增加。总的来说，冷等离子体处理PH值为0.60% （W/V）的cofi在功能上是最有利的。

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引用次数: 0

Pomegranate peel powder and extract improved weight control, lipid metabolism and gut microbiota in hamsters fed with standard american diets 石榴皮粉末和提取物改善了美国标准饮食喂养仓鼠的体重控制、脂质代谢和肠道微生物群

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-16 DOI: 10.1016/j.fhfh.2025.100196

Xingzhu Wu , Wallace Yokoyama , Yuqing Tan , Glenn Bartley , Ling Chen , James Pan , Priscila Alves Buongiorno , Jose Berrios , Tara McHugh , Zhongli Pan

Pomegranate peel waste is a byproduct of juice processing and 1.6 million tons are produced globally each year. Pomegranate peels have a high dietary fiber content and unique polyphenol profile suggesting it may have health benefits. This study aimed to investigate the hypocholesterolemic and anti-obesity effects of pomegranate peel powder (PPP) and water extract (PPE) in Golden Syrian hamsters fed with high-fat (39 % fat calorie, HF) diets. Hamsters were fed either the HF, or the HF diet supplemented with 2.5 % or 5 % PPE (LE and HE, respectively), or 5 % or 10 % PPP (low-peel (LP) and high-peel (HP), respectively. After 4 weeks of feeding, hamsters gained 16.10- 33.82 g of weight and the feeding efficacy ranged from 0.10 to 0.18. The HP group had the lowest weight gain and feed efficacy while others were not significantly different. The HP group had significantly lower liver-to-body weight ratio (3.10 ± 0.08 % vs 3.65 ± 0.09 %), fasting blood glucose (68.11 ± 5.27 vs 82.94 ± 6.49 mg/dL), and hepatic lipid content (6.31 ± 0.26 vs 7.49 ± 0.22 g/100 g liver) compared to the HF group. PPP ingestion significantly increased LDL but decreased triglycerides. PPP and PPE feeding resulted in microbiota phyla Firmicutes-to-Bacteroidetes ratio characteristic of leaner phenotypes. HMG-CoAR and LDLR expression were reduced, suggesting that decreased uptake of LDL was not sufficient to lower plasma LDL, even with reduced cholesterol synthesis.

石榴皮废料是果汁加工的副产品，全球每年产生160万吨。石榴皮含有很高的膳食纤维含量和独特的多酚成分，这表明石榴皮可能对健康有益。本研究旨在探讨石榴皮粉（PPP）和水提物（PPE）对高脂肪（39%脂肪热量，HF）饲粮喂养的叙利亚金仓鼠的降胆固醇和抗肥胖作用。分别饲喂HF、HF饲粮中添加2.5%或5% PPE（分别为LE和HE）、5%或10% PPP（分别为低削皮（LP）和高削皮（HP））的仓鼠。饲喂4周后，仓鼠增重16.10 ~ 33.82 g，饲喂效率为0.10 ~ 0.18。HP组的增重和饲料效率最低，其他组差异不显著。与HF组相比，HP组的肝体重比（3.10±0.08% vs 3.65±0.09%）、空腹血糖（68.11±5.27 vs 82.94±6.49 mg/dL）和肝脏脂质含量（6.31±0.26 vs 7.49±0.22 g/100 g肝脏）均显著降低。PPP摄入显著增加LDL，但降低甘油三酯。PPP和PPE饲养导致厚壁菌门与拟杆菌门的微生物比例呈较瘦表型特征。HMG-CoAR和LDLR表达降低，表明LDL摄取减少并不足以降低血浆LDL，即使胆固醇合成减少。

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引用次数: 0

Encapsulation of anthocyanins from purple corn cob via antisolvent precipitation: Effect of pH and zein/gum arabic ratio on the antioxidant activity, particle size and thermal stability 抗溶剂沉淀法包封紫玉米芯花青素：pH和玉米蛋白/阿拉伯胶比对抗氧化活性、粒径和热稳定性的影响

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-16 DOI: 10.1016/j.fhfh.2025.100197

Johan Mendoza , Omar Peñuñuri-Miranda , María d.C. Valdez-Cárdenas , Carmen O. Melendez-Pizarro , Daniel Lardizabal-Gutiérrez , Francisco Paraguay-Delgado , Armando Quintero-Ramos

This study evaluates the effects of pH levels and Zein/Gum Arabic (Z/GA) ratios on key encapsulation parameters of an anthocyanin-rich extract (ARE) derived from purple corn cob, using the antisolvent precipitation method. Parameters analyzed include encapsulation efficiencies for anthocyanins (%AEE) and polyphenols (%PEE), nanoparticle size, polydispersity index, ζ-potential, and thermal stability at 80 and 180 °C. Particles without GA showed poor stability and low %AEE, particularly at lower pH. Conversely, the addition of GA significantly enhanced encapsulation efficiency, especially under acidic conditions (pH 2–4), and improved the particle size uniformity. At Z/GA ratio of 1:1, GA played a crucial role in stabilizing nanoparticles, effectively preventing aggregation even when the net particle charge was near to zero. Characterization by SEM, FTIR and TGA confirmed the morphological, structural, and thermal properties of the encapsulated particles. Thermal stability tests demonstrated that encapsulated anthocyanins exhibited significantly improved resistance to thermal degradation, with half-life extended up to threefold compared to unencapsulated counterparts. These results highlight the potential of encapsulating ARE from purple corn cob in Z/GA matrices as a method to preserve anthocyanins functionality, improve their thermal stability during food processing, and enhance application in food systems. Additionally, this approach offers a sustainable alternative, adding value to agricultural by-products and promoting waste valorization in the food industry. However, further research on scalability, cost-effectiveness, and application in food processing systems are needed.

采用抗溶剂沉淀法，研究了pH和玉米苷/阿拉伯胶（Z/GA）比对紫玉米芯花青素提取物（ARE）关键包封参数的影响。分析的参数包括花青素（%AEE）和多酚（%PEE）的包封效率、纳米颗粒尺寸、多分散性指数、ζ-电位以及80和180℃下的热稳定性。未添加GA的颗粒稳定性差，AEE %低，特别是在较低的pH下。相反，添加GA显著提高了包封效率，特别是在酸性条件下（pH 2-4），并改善了粒径均匀性。当Z/GA比为1:1时，GA在稳定纳米颗粒方面发挥了至关重要的作用，即使在粒子净电荷接近于零的情况下，GA也能有效地防止纳米颗粒聚集。通过扫描电镜（SEM）、红外光谱（FTIR）和热重分析仪（TGA）等表征手段证实了包封颗粒的形态、结构和热性能。热稳定性测试表明，与未封装的花青素相比，封装后的花青素表现出明显改善的耐热降解能力，半衰期延长了三倍。这些结果强调了将紫玉米芯中的ARE包埋在Z/GA基质中作为一种保存花青素功能、提高其在食品加工过程中的热稳定性和增强其在食品体系中的应用的潜力。此外，这种方法提供了一种可持续的替代方案，增加了农业副产品的价值，并促进了食品工业中的废物增值。然而，在可扩展性、成本效益和在食品加工系统中的应用方面还需要进一步的研究。

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引用次数: 0

Emulsion electrospinning of lemongrass essential Oil-Loaded Ferula haussknechtii gum/ Polyethylene oxide as bioactive coating 柠檬草精油负载阿魏胶/聚乙烯氧化物作为生物活性包被的乳液静电纺丝研究

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-01-07 DOI: 10.1016/j.fhfh.2025.100195

Saeedeh Jafari, Abdollah Hematian Sourki, Safoora Pashangeh

This study aimed to develop a novel, degradable antimicrobial bioactive coating by combining Ferula haussknechtii gum and polyethylene oxide with lemongrass essential oil (LGEO) using the emulsion electrospinning technique. The LGEO emulsion in the F. haussknechtii gum/polyethylene oxide solution was prepared via ultrasonic method, and its physicochemical properties, including pH, electrical conductivity, density, and apparent viscosity, were systematically evaluated. The microstructural morphology of the electrospun coating was analyzed using scanning electron microscopy (SEM). The antimicrobial properties and antioxidant potential of the active electrospun coating were also assessed. The resulting electrospun fibers had an average diameter of 0.56 μm and contained over 36 bioactive compounds, exhibiting radical scavenging activity of approximately 74.51 %. The LGEO was incorporated into the bioactive coating at concentrations of 3, 6, and 9 % (v/v). The antimicrobial efficacy of the electrospun coating was tested against Gram-positive Gram-negative, and Aspergillus niger. The highest antimicrobial activity was observed with the electrospun coating containing 9 % LGEO. The results revealed that increasing the LGEO concentration in the emulsion resulted in decreased pH, apparent viscosity, and density, while electrical conductivity increased. SEM analysis confirmed the formation of uniform, bead-free electrospun fibers across all LGEO concentrations. FTIR analysis validated the successful incorporation of emulsified LGEO into the electrospun fibers. These findings demonstrate that the inclusion of LGEO in bioactive edible coatings can significantly enhance antimicrobial protection, particularly for minimally processed foods, while potentially extending shelf life by reducing microbial contamination.

本研究采用乳化液静电纺丝技术，将阿魏胶、聚乙烯氧化物与柠檬草精油结合，制备一种新型的、可降解的抗菌生物活性涂层。采用超声法制备了聚乙二醇/聚氧乙烯溶液中的LGEO乳液，并对其理化性质（pH、电导率、密度、表观粘度）进行了系统评价。利用扫描电子显微镜（SEM）分析了静电纺丝涂层的微观组织形态。并对活性静电纺涂层的抗菌性能和抗氧化潜力进行了评价。所得静电纺纤维平均直径为0.56 μm，含有36种以上的生物活性化合物，自由基清除活性约为74.51%。LGEO分别以3%、6%和9% （v/v）的浓度掺入生物活性包被中。测定了静电纺涂层对革兰氏阳性菌、革兰氏阴性菌和黑曲霉的抑菌效果。结果表明，含9% LGEO的电纺丝涂层抗菌活性最高。结果表明，随着LGEO浓度的增加，乳液的pH值、表观粘度和密度降低，电导率提高。扫描电镜分析证实，在所有LGEO浓度下，形成了均匀、无珠的静电纺丝纤维。FTIR分析证实了乳化LGEO成功掺入到电纺丝纤维中。这些发现表明，在生物活性食用涂层中加入LGEO可以显著增强抗菌保护，特别是对于最低限度加工的食品，同时可能通过减少微生物污染来延长保质期。

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