Food Hydrocolloids for Health最新文献

The present work was planned to optimize extraction process of phenolics, flavonoids, monomeric anthocyanin and antioxidants from black rice bran using Ultrasound-assisted extraction and finally utilize it for encapsulation using double emulsion coacervation process. Response surface methodology (RSM) was employed for optimization of extraction process. Acidified ethanol (1 M HCl) was used as extraction solvent and ultrasound power (W) and extraction time (min) were used as independent variables. Face centered composite design (FCCD) data was successfully opted and developed a 2nd order polynomial equation with R² value of 0.94 for total phenolic content (TPC), 0.95 for total flavonoid content (TFC), 0.87 for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 0.98 for total anthocyanin content (ANCs) respectively. Kinetic model study for extraction of anthocyanin was compared to conventional extraction process. Characterization of the extracts was performed using high pressure liquid chromatography analysis (HPLC). Cyanidin-3-glucoside is the predominated anthocyanin found as compared to peonidin-3-D-glucoside. Finally, coacervated microcapsules were developed using anthocyanin extract as core solution and gelatin and acacia gum as wall materials. Characterization of the microcapsules in terms of moisture content, hygroscopicity, solubility, encapsulation efficiency and surface morphology were evaluated. The result thus obtained shows that potential anthocyanin content from black rice bran can be utilized as food ingredients for various food application and formulation of functional food.

In this study, Vicia villosa protein isolate (VVPI), with a high and valuable protein source, was used to create an edible coating containing ZnO nanoparticles (ZnO NPs) to investigate the effect of active coating on the quality of chicken breast fillets during refrigerated storage. The results showed a low growth rate in total viable count (TVC) and lactic acid bacteria (LAB) in coated samples. Moreover, as ZnO NPs concentration increased, thiobarbituric acid reactive substances (TBARS) decreased in the coated samples. In addition, the acid value and total volatile basic nitrogen (TVB-N) in the coated samples were significantly lower than the control group. Fourier transform infrared (FTIR) spectrum confirmed the chemical interactions of the coating components. Scanning electron microscopy (SEM) showed a homogeneous layer of coating on the surface of the coated chicken meat. Organoleptic indicators including color, smell, texture and overall acceptability were acceptable in all coated samples. These findings showed that the coating based on protein isolate containing ZnO NPs has a good potential to increase the safety and shelf life of chicken meat.

Plant-derived hydrolysates are emerging as promising agents in the management of diverse ailments due to their ensuing functional and bioactive properties. This study investigated the functional, antioxidant, anti-inflammatory, and antidiabetic properties of hydrolysates from Erythrina senegalensis and Vigna subtenarrea seeds. Crude proteins were isolated via alkaline solubilization, followed by acid precipitation to the isoelectric point. Respective protein isolates were hydrolyzed using trypsin and pepsin at an enzyme-substrate ratio of 1:8 (v/v) for 1–9 h. Pepsin hydrolysates after 9 h elicited the highest solubilities of 95.54 % and 94.24 % at pH 13, while, pepsin and trypsin hydrolysates of E. senegalensis digested for 1 and 9 h displayed the highest 1,1-diphenyl-2-picrylhydrazyl radical-scavenging (10-IC₅₀ = 2.959 mg/mL) and total antioxidant capacity (7.243 mgAAE/g), respectively. Correspondingly, pepsin and trypsin hydrolysates of E. senegalensis hydrolyzed for 5 h demonstrated the most potent anti-inflammatory activities by cogently inhibiting xanthine oxidase and lipoxygenase activities with IC₅₀ of 0.161 ± 0.111 and 0.018 ± 0.011 mg/mL, respectively. Trypsin hydrolysates of V. subterranea hydrolyzed for 5 h potently inhibited the activities of α-amylase and α-glucosidase with respective IC₅₀ of 0.297 ± 0.060 and 0.314 ± 0.064 mg/mL. Overall, pepsin and trypsin hydrolysates of E. senegalensis demonstrated pronounced functional bioactivities relative to V. subterranea hydrolysates. This study concludes that both seeds could serve as unique matrices of potential functional bioactive peptides with prospects for managing diabetes.

Ascorbic acid (AA) is a vital nutrient to maintain critical physiological functions but is very sensitive to processing and storage. This can be overcome by using gel-based systems for controlled release of AA. This study compares various gel-based formulations such as hydrogel, emulsion gel, bigel (25 %, 50 %, and 75 % oleogel), and emulsions for thermal stability and delivery of AA, rheological and textural profile, encapsulation efficiency (>97 %), in vitro gastrointestinal release profile, and the corresponding antioxidant profile. An increase in the oleogel content increased the hardness (125 – 216 g) and viscoelastic properties (G′ and G′′) but decreased (76.16 – 25.86 %) the swelling ratio of the bigel. A spontaneous release of AA was witnessed during gastric digestion from emulsion gels (95 %), hydrogels (98 %) and emulsions, whereas a gradual and controlled gastric release of AA could be achieved by bigels. However, a sudden decrease in AA (70 – 80 % reduction) and a spike in dehydroascorbic acid (DHA, oxidized AA) could be observed during intestinal digestion. The bioaccessibility was highest for emulsion gel and bigel (87 %) and lowest for emulsions (70 %). Bigels with higher oleogel content also showed better thermal stability but their physical stability was compromised at higher temperature. The DPPH and ABTS activity was proportional to AA, while FRAP was impacted by both DHA and AA. Thus bigels could be utilised for controlled gastric release of AA with better thermal stability.

Oat β-glucan is a prebiotic that affects intestinal microbiota and maintains energy homeostasis. Oat β-glucan helps treat diarrhea and other associated disorders, because it can regulate intestinal microbiota. We investigated the therapeutic effects and mechanism of oat β-glucan combined with montmorillonite powder on diarrhea induced by Senna leaf extract in young Sprague-Dawley rats. Compared with the diarrhea-induced model group (DM), the combined treatment, especially in the group treated with a medium dose of Montmorillonite powder plus oat β-glucan (M+G2), effectively reduced the diarrhea (P < 0.05) and inflammation indices, alleviated damage to the colon, and promoted weight gain in rats. In the combined treatment group, the relative abundance of Firmicutes increased at the phylum level, while the relative abundance of Proteobacteria and Actinobacteria decreased. At the genus level, the Lactobacillus content recovered, and the proportion of conditional pathogens, such as Prevotella and Paraprevotella decreased. M+G2 treatment significantly reduced diarrhea in young rats, restored intestinal microbiota diversity, and promoted the production of short-chain fatty acids (SCFAs). Based on metabolomics, the mechanism of the anti-diarrheal effect of M+G2 treatment may be related to the regulation of glucose and amino acid metabolism. The metabolic micro-environment was improved through the pentose phosphate and vitamin B6 pathways. The core metabolic regulator in metabolic network analysis was L-aspartic acid. Overall, our findings suggest that the combined treatment of oat β-glucan and montmorillonite powder may provide an effective therapeutic strategy for treating diarrhea and associated disorders by regulating the inflammatory biomarkers, SCFAs, and intestinal microbiota.

Keeping in view the progressive future prospectus of carbohydrate research, herein, the potential of bioactive polysaccharides aloe vera (AV) and sterculia gum (SG) was explored to develop network hydrogel wound dressings. Both polysaccharides, acemannan of AV gel and glucuronic-galacturonic acid of SG accelerate wound healing process. The antibiotic drug cefuroxime encapsulated dressings were designed by graft-copolymerization with carbopol. The copolymers were characterized by SEM, AFM, FTIR, C¹³ NMR, XRD, TGA-DTG, DSC and swelling studies. The slow diffusion of cefuroxime drug exhibited non-Fickian mechanism and release of drug was occurred with a first order kinetic model. Polymer-bio membrane interactions indicated mucoadhesion nature. The clot formation was reduced during polymer-blood interactions indicated bio-compatible nature of the dressings moreover dressings were found antioxidant in nature. The porous dressings were O₂ permeable and microbial impermeable. The hydrogel dressings revealed antimicrobial activity against P. aeruginosa. Degree of antimicrobial activity enhanced in case of hydrogel wound dressings loaded with cefuroxime. Overall, the results of these biochemical assays, sustained release of antibiotic drug and inherent wound healing potential of AV-SG indicated the use of hydrogels as dressing materials for better wound healing.

Modifying the complex wall structure of bee pollen is a way to enhance the uptake of bioactive compounds. Bee Pollen and Bee Honey hybrid formulation (BPHF) are made by hot melt extrusion (HME), which is known to be the best cell wall structure-breaking method for active compound extraction because it can break the fibrous matrix of the compounds. Honey and bee pollen mixtures showed increased antioxidant activity with increased phenolic and flavonoid contents and decreased inhibitory concentration 50 (IC₅₀) values compared to the control group. In addition, the content of gallic acid, one of the phenolic acids, increased about four times in the HME-F3 formulation compared to the control group, indicating that the phenol content was rich, but the HME-F3 formulation decreased in comparison. All samples had nano-sized particles. The Polydispersity index (PDI) value of the HME-F3 formulation was reduced and well distributed, and the stability of the particles was confirmed in the zeta potential results. Scanning electron microscopy (SEM) showed the image change of the sample after HME compared to the existing bee pollen image, and Transmission electron microscopy (TEM) showed that the particles of the mixed formulation were uniformly dispersed. Fourier-transform Infrared Analysis (FT-IR) results showed that there were structural changes in the mixture formulation compared to the control, and X-ray Diffraction Analysis (XRD) results showed that all samples were amorphous. It was found that the HME-F3 formulation could resist inflammation and protect the function of the intestinal epithelial wall. The mRNA levels of TJ-related proteins in the mixed formulation were reduced compared to LPS treatment and control.