Food Hydrocolloids for Health最新文献_第2页

Hypoglycemic effect of W/O/W emulsion encapsulating Polygonatum sibiricum polysaccharides and saponins in a mouse model of T2DM 包埋黄精多糖和皂苷的W/O/W乳剂对T2DM小鼠模型的降糖作用

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-11-29 DOI: 10.1016/j.fhfh.2025.100259

Mi Li , Yunge Zhang , Quanlin Li , Bingzong Xie , Jian Ju , Yanli Ma

This study explores the hypoglycemic efficacy of W/O/W emulsion co-encapsulating Polygonatum sibiricum polysaccharides (PSP) and saponins (PSS). In streptozotocin/high-fat diet-induced type 2 diabetes mellitus (T2DM) mice, the encapsulated PSP/PSS exhibited excellent activity: reversing typical "three more and one less" symptoms, and significantly improving blood glucose, glucose tolerance, insulin resistance, lipids, liver oxidative stress, liver glycogen, and liver/kidney morphology. After 4 weeks of intervention, the fasting blood glucose (FBG) reduction rate of the co-encapsulation group (PPE) was 20%, the homeostasis model assessment of insulin resistance (HOMA-IR) decreased by 35%, and the hepatic glycogen content increased by 122%. Meanwhile, PSP/PSS exerted effects by regulating intestinal beneficial microbiota, Akkermansia and Lactobacillus abundances in the PPE group were 1.8-fold and 2.3-fold higher than those in the diabetic model group (DC), respectively, which was more significant than the microbial regulatory effect of the PSP-only emulsion group (PE). Compared with PE group, the co-encapsulation system showed more prominent efficacy, highlighting multi-component synergistic delivery advantages. This study first confirms that W/O/W emulsion-based PSP/PSS delivery system effectively improves diabetic pathology, providing an innovative paradigm for integrating novel functional food delivery technologies with traditional medicine-food homologous plants.

本研究探讨了黄精多糖（PSP）和皂苷（PSS）共包埋W/O/W乳液的降糖效果。在链脲佐菌素/高脂饮食诱导的2型糖尿病（T2DM）小鼠中，包封的PSP/PSS表现出优异的活性：逆转典型的“三多一少”症状，显著改善血糖、葡萄糖耐量、胰岛素抵抗、血脂、肝脏氧化应激、肝糖原和肝肾形态。干预4周后，共胶囊组（PPE）空腹血糖（FBG）降低率为20%，胰岛素抵抗稳态模型评估（HOMA-IR）降低35%，肝糖原含量升高122%。同时，PSP/PSS通过调节肠道有益菌群发挥作用，PPE组的Akkermansia和Lactobacillus丰度分别比糖尿病模型组（DC）高1.8倍和2.3倍，比仅PSP乳状液组（PE）的微生物调节作用更为显著。与PE组相比，共胶囊体系的功效更加突出，突出了多组分协同递送的优势。本研究首次证实了W/O/W乳化液为基础的PSP/PSS给药系统能有效改善糖尿病病理，为将新型功能性食品给药技术与传统药食同源植物相结合提供了创新范例。

{"title":"Hypoglycemic effect of W/O/W emulsion encapsulating Polygonatum sibiricum polysaccharides and saponins in a mouse model of T2DM","authors":"Mi Li , Yunge Zhang , Quanlin Li , Bingzong Xie , Jian Ju , Yanli Ma","doi":"10.1016/j.fhfh.2025.100259","DOIUrl":"10.1016/j.fhfh.2025.100259","url":null,"abstract":"<div><div>This study explores the hypoglycemic efficacy of W/O/W emulsion co-encapsulating <em>Polygonatum sibiricum</em> polysaccharides (PSP) and saponins (PSS). In streptozotocin/high-fat diet-induced type 2 diabetes mellitus (T2DM) mice, the encapsulated PSP/PSS exhibited excellent activity: reversing typical \"three more and one less\" symptoms, and significantly improving blood glucose, glucose tolerance, insulin resistance, lipids, liver oxidative stress, liver glycogen, and liver/kidney morphology. After 4 weeks of intervention, the fasting blood glucose (FBG) reduction rate of the co-encapsulation group (PPE) was 20%, the homeostasis model assessment of insulin resistance (HOMA-IR) decreased by 35%, and the hepatic glycogen content increased by 122%. Meanwhile, PSP/PSS exerted effects by regulating intestinal beneficial microbiota, <em>Akkermansia</em> and <em>Lactobacillus</em> abundances in the PPE group were 1.8-fold and 2.3-fold higher than those in the diabetic model group (DC), respectively, which was more significant than the microbial regulatory effect of the PSP-only emulsion group (PE). Compared with PE group, the co-encapsulation system showed more prominent efficacy, highlighting multi-component synergistic delivery advantages. This study first confirms that W/O/W emulsion-based PSP/PSS delivery system effectively improves diabetic pathology, providing an innovative paradigm for integrating novel functional food delivery technologies with traditional medicine-food homologous plants.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"9 ","pages":"Article 100259"},"PeriodicalIF":5.1,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145697915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a new self-nanoemulsifying Narcissus tazetta L. oil delivery system and study of its anti-breast and anti-pancreas cancers activities in vitro 新型自纳米乳化水仙油传递体系的研制及其体外抗乳腺癌、抗胰腺癌活性研究

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-11-14 DOI: 10.1016/j.fhfh.2025.100255

R. Nazari-Vanani , S.R. Rasouli Nasrabadi , S.A. Dastgheib , H. Heli

Administration of natural oil extracts suffers from low oral bioavailability and anti-proliferative activity. Self-nanoemulsifying drug delivery systems (SNEDDSs) are novel formulations to improvement of the efficacy of poorly water-soluble compounds. In this study, a novel Narcissus Tazetta L. oil-loaded SNEDDS (NT-SNEDDS) was used to improve the antitumor effect of the oil against MCF-7 and PANC-1 cells. 30% tween 80, 55 % PEG 600, and 15 % ethyl oleate were considered as surfactant, cosurfactant, and oil, respectively, to form a SNEDDS. When combined with water, NT-SNEDDS created a nanoemulsion consisting of oil nanodroplets of 40±8 nm. Field emission scanning electron microscopy was also utilized to examine the structure of the nanodroplets. The formulations exhibited no indications of precipitation or phase separation throughout the storage duration. Toxicity of NT-SNEDDS was evaluated, and the results suggested that it had potential as an anticancer with IC50 values of 0.17 and 0.21 % (V/V, Narcissus Tazetta L. oil content of NT-SNEDDS) against MCF-7 and PANC-1 cells, respectively. The enhanced cytotoxicity was due to the induction of DNA fragmentation and apoptosis. Further investigations through in vitro mechanistic endpoint as well as in vivo experimentations would clarify the efficacy of NT-SNEDDS.

天然油提取物的口服生物利用度和抗增殖活性较低。自纳米乳化给药系统（snedds）是一种改善难水溶性化合物药效的新剂型。本研究利用一种新型水仙油负载SNEDDS （NT-SNEDDS）提高水仙油对MCF-7和PANC-1细胞的抗肿瘤作用。将30%的二甲苯80、55%的PEG 600和15%的油酸乙酯分别作为表面活性剂、助表面活性剂和油，形成SNEDDS。当与水结合时，NT-SNEDDS形成了由40±8 nm的油纳米滴组成的纳米乳液。利用场发射扫描电镜对纳米液滴的结构进行了表征。在整个贮存期间，制剂没有表现出沉淀或相分离的迹象。结果表明，NT-SNEDDS对MCF-7和PANC-1细胞的IC50值分别为0.17和0.21% （V/V，水仙油含量）。细胞毒性的增强是由于DNA断裂和细胞凋亡的诱导。通过体外机制终点和体内实验的进一步研究将阐明NT-SNEDDS的疗效。

{"title":"Development of a new self-nanoemulsifying Narcissus tazetta L. oil delivery system and study of its anti-breast and anti-pancreas cancers activities in vitro","authors":"R. Nazari-Vanani , S.R. Rasouli Nasrabadi , S.A. Dastgheib , H. Heli","doi":"10.1016/j.fhfh.2025.100255","DOIUrl":"10.1016/j.fhfh.2025.100255","url":null,"abstract":"<div><div>Administration of natural oil extracts suffers from low oral bioavailability and anti-proliferative activity. Self-nanoemulsifying drug delivery systems (SNEDDSs) are novel formulations to improvement of the efficacy of poorly water-soluble compounds. In this study, a novel <em>Narcissus Tazetta</em> L. oil-loaded SNEDDS (NT-SNEDDS) was used to improve the antitumor effect of the oil against MCF-7 and PANC-1 cells. 30% tween 80, 55 % PEG 600, and 15 % ethyl oleate were considered as surfactant, cosurfactant, and oil, respectively, to form a SNEDDS. When combined with water, NT-SNEDDS created a nanoemulsion consisting of oil nanodroplets of 40±8 nm. Field emission scanning electron microscopy was also utilized to examine the structure of the nanodroplets. The formulations exhibited no indications of precipitation or phase separation throughout the storage duration. Toxicity of NT-SNEDDS was evaluated, and the results suggested that it had potential as an anticancer with IC50 values of 0.17 and 0.21 % (V/V, <em>Narcissus Tazetta</em> L. oil content of NT-SNEDDS) against MCF-7 and PANC-1 cells, respectively. The enhanced cytotoxicity was due to the induction of DNA fragmentation and apoptosis. Further investigations through in vitro mechanistic endpoint as well as in vivo experimentations would clarify the efficacy of NT-SNEDDS.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100255"},"PeriodicalIF":5.1,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulation of bifidobacteria in natural biopolymers: Advances, challenges, and applications in dairy products 双歧杆菌在天然生物聚合物中的包封：进展、挑战和在乳制品中的应用

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-11-04 DOI: 10.1016/j.fhfh.2025.100252

Marko Vinceković , Lana Živković , Botagoz Mutaliyeva , Galiya Madybekova , Elmira Turkeyeva , Nenad Jalšenjak , Assem Issayeva

Encapsulation of Bifidobacteria with natural biopolymers has emerged as a promising strategy to enhance the viability of probiotics in dairy systems. While Bifidobacteria confer well-established health benefits, their industrial application is hindered by sensitivity to processing and storage conditions. This review critically evaluates encapsulation methods, including extrusion, emulsification, spray drying, and freeze drying, as well as natural polymers such as alginate, chitosan, carrageenan, gelatin, and starch, with a focus on their ability to maintain the stability of probiotics. Beyond summarizing current techniques, we highlight persisting gaps in scalability, cost efficiency, and consumer acceptance, issues often overlooked in earlier reviews. By integrating recent advances in material science with food engineering perspectives, this article outlines pathways for industrial translation. It distinguishes itself by focusing on how encapsulation strategies can be optimized to overcome these barriers, ultimately enabling broader adoption of probiotic-enriched dairy products.

用天然生物聚合物封装双歧杆菌已成为一种有前途的策略，以提高益生菌在乳制品系统中的生存能力。虽然双歧杆菌具有公认的健康益处，但它们对加工和储存条件的敏感性阻碍了它们的工业应用。本文综述了包括挤压、乳化、喷雾干燥和冷冻干燥在内的封装方法，以及海藻酸盐、壳聚糖、卡拉胶、明胶和淀粉等天然聚合物，重点介绍了它们保持益生菌稳定性的能力。除了总结当前的技术之外，我们还强调了在可伸缩性、成本效率和消费者接受度方面持续存在的差距，这些问题在早期的评论中经常被忽视。通过将材料科学的最新进展与食品工程观点相结合，本文概述了工业翻译的途径。它的特点是专注于如何优化封装策略来克服这些障碍，最终使益生菌丰富的乳制品得到更广泛的采用。

{"title":"Encapsulation of bifidobacteria in natural biopolymers: Advances, challenges, and applications in dairy products","authors":"Marko Vinceković , Lana Živković , Botagoz Mutaliyeva , Galiya Madybekova , Elmira Turkeyeva , Nenad Jalšenjak , Assem Issayeva","doi":"10.1016/j.fhfh.2025.100252","DOIUrl":"10.1016/j.fhfh.2025.100252","url":null,"abstract":"<div><div>Encapsulation of <em>Bifidobacteria</em> with natural biopolymers has emerged as a promising strategy to enhance the viability of probiotics in dairy systems. While <em>Bifidobacteria</em> confer well-established health benefits, their industrial application is hindered by sensitivity to processing and storage conditions. This review critically evaluates encapsulation methods, including extrusion, emulsification, spray drying, and freeze drying, as well as natural polymers such as alginate, chitosan, carrageenan, gelatin, and starch, with a focus on their ability to maintain the stability of probiotics. Beyond summarizing current techniques, we highlight persisting gaps in scalability, cost efficiency, and consumer acceptance, issues often overlooked in earlier reviews. By integrating recent advances in material science with food engineering perspectives, this article outlines pathways for industrial translation. It distinguishes itself by focusing on how encapsulation strategies can be optimized to overcome these barriers, ultimately enabling broader adoption of probiotic-enriched dairy products.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100252"},"PeriodicalIF":5.1,"publicationDate":"2025-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical fish scale collagen cream: Formulation, skin permeation, and biocompatibility for cosmetic and therapeutic use 外用鱼鳞胶原蛋白霜：化妆品和治疗用的配方、皮肤渗透性和生物相容性

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-11-03 DOI: 10.1016/j.fhfh.2025.100253

Ismail Zainol , Ayah Rebhi Hilles , Wan Rusmawati Wan Mahamod , Hasnor Izzati Che Razali , Haniza Hanim Mohd Zain

This study aims to develop and characterise fish scale-collagen cream (CoC) intended for topical application. The development of CoC consists of hydrolysed collagen (molecular weight ≈ 1 kDa) dispersed into the aqueous phase of a water-in-oil (w/o) emulsion of Span 60: Tween 60/water/virgin coconut oil (VCO) system. Collagen used in this study has been extracted from Tilapia fish scales using hydrothermal extraction combined with enzymatic hydrolysis treatment. The CoC was developed using a water-in-VCO emulsion medium at water volume fractions (φ_w) of 0.74, 0.83, and 0.94. Up to 20 wt.% of hydrolysed collagen was successfully loaded into the emulsion system via the high shear homogenisation technique. The characterisation of the CoC involved a complex microscopic analysis, including optical polarising microscopy (OPM) and scanning electron microscopy (SEM), complemented by droplet size analysis, differential scanning calorimetry, and rheological evaluation. The physical characteristics of CoC were investigated to evaluate its suitability for administration on the skin. The potential of CoC to be applied on the skin was evaluated using analysis of ex vivo rat skin permeation using the Franz diffusion cell, in vitro fibroblast cell viability and in vivo toxicity to the mice. Franz diffusion cell studies revealed 3466.46 ± 21.70 μg/cm² cumulative collagen permeation over 6 h (vs. 1750 ± 319.67 μg/cm² control; p < 0.001, 95 % CI: 1580–1850 μg/cm²), with 94–97 % retention in skin layers. In vitro, 0.02 mg/mL hydrolysed collagen increased fibroblast viability by 34 % (p < 0.01, n = 3). Furthermore, Sub-acute toxicity tests of 25–50 mg/kg body weight of hydrolysed collagen for 7 days showed no significant weight changes (p > 0.05, 95 % CI: −0.5 % to +1.2 %) or mortality. It was observed that the hydrolysed collagen could pass through the rat skin easily and successfully stimulate the fibroblast cell viability. In conclusion, CoC is a suitable carrier for hydrolysed collagen in topical applications. The use of the designed emulsion-based creams can safely enhance the delivery of the hydrolysed collagen in the skin layers with additional benefits for cosmetic and therapeutic applications.

本研究旨在开发和表征用于外用的鱼鳞胶原蛋白霜（CoC）。CoC的制备由水解的胶原蛋白（分子量≈1 kDa）分散在Span 60: Tween 60/水/初榨椰子油（VCO）体系的油包水（w/o）乳液的水相中组成。本研究使用的胶原蛋白是从罗非鱼鱼鳞中提取的，采用水热萃取结合酶解处理。CoC是在水体积分数（φw）分别为0.74、0.83和0.94的水- vco乳液介质中制备的。通过高剪切均质技术，高达20%的水解胶原蛋白被成功地装入乳液系统。CoC的表征涉及复杂的显微分析，包括光学偏光显微镜（OPM）和扫描电子显微镜（SEM），辅以液滴尺寸分析、差示扫描量热法和流变学评估。研究了CoC的物理特性，以评价其在皮肤上给药的适用性。利用Franz扩散细胞分析体外大鼠皮肤渗透性、体外成纤维细胞活力和对小鼠的体内毒性，评估CoC在皮肤上的应用潜力。Franz扩散池研究显示，6小时内累积胶原渗透3466.46±21.70 μg/cm²（对照组为1750±319.67 μg/cm²；p < 0.001, 95% CI: 1580-1850 μg/cm²），在皮肤层中保留率为94 - 97%。在体外，0.02 mg/mL水解胶原可使成纤维细胞活力提高34% （p < 0.01, n = 3）。此外，25-50 mg/kg体重水解胶原蛋白7天的亚急性毒性试验显示，体重没有显著变化（p > 0.05, 95% CI: - 0.5%至+ 1.2%）或死亡率。结果表明，水解后的胶原蛋白能很容易地穿过大鼠皮肤，并能成功地刺激成纤维细胞的活力。综上所述，CoC是一种适合于外用的水解胶原蛋白载体。使用设计的乳化乳霜可以安全地增强皮肤层中水解胶原蛋白的输送，并为美容和治疗应用带来额外的好处。

{"title":"Topical fish scale collagen cream: Formulation, skin permeation, and biocompatibility for cosmetic and therapeutic use","authors":"Ismail Zainol , Ayah Rebhi Hilles , Wan Rusmawati Wan Mahamod , Hasnor Izzati Che Razali , Haniza Hanim Mohd Zain","doi":"10.1016/j.fhfh.2025.100253","DOIUrl":"10.1016/j.fhfh.2025.100253","url":null,"abstract":"<div><div>This study aims to develop and characterise fish scale-collagen cream (CoC) intended for topical application. The development of CoC consists of hydrolysed collagen (molecular weight ≈ 1 kDa) dispersed into the aqueous phase of a water-in-oil (w/o) emulsion of Span 60: Tween 60/water/virgin coconut oil (VCO) system. Collagen used in this study has been extracted from Tilapia fish scales using hydrothermal extraction combined with enzymatic hydrolysis treatment. The CoC was developed using a water-in-VCO emulsion medium at water volume fractions (φ<sub>w</sub>) of 0.74, 0.83, and 0.94. Up to 20 wt.% of hydrolysed collagen was successfully loaded into the emulsion system via the high shear homogenisation technique. The characterisation of the CoC involved a complex microscopic analysis, including optical polarising microscopy (OPM) and scanning electron microscopy (SEM), complemented by droplet size analysis, differential scanning calorimetry, and rheological evaluation. The physical characteristics of CoC were investigated to evaluate its suitability for administration on the skin. The potential of CoC to be applied on the skin was evaluated using analysis of <em>ex vivo</em> rat skin permeation using the Franz diffusion cell, <em>in vitro</em> fibroblast cell viability and <em>in vivo</em> toxicity to the mice. Franz diffusion cell studies revealed 3466.46 ± 21.70 μg/cm² cumulative collagen permeation over 6 h (vs. 1750 ± 319.67 μg/cm² control; <em>p</em> < 0.001, 95 % CI: 1580–1850 μg/cm²), with 94–97 % retention in skin layers. <em>In vitro</em>, 0.02 mg/mL hydrolysed collagen increased fibroblast viability by 34 % (<em>p</em> < 0.01, <em>n</em> = 3). Furthermore, Sub-acute toxicity tests of 25–50 mg/kg body weight of hydrolysed collagen for 7 days showed no significant weight changes (<em>p</em> > 0.05, 95 % CI: −0.5 % to +1.2 %) or mortality. It was observed that the hydrolysed collagen could pass through the rat skin easily and successfully stimulate the fibroblast cell viability. In conclusion, CoC is a suitable carrier for hydrolysed collagen in topical applications. The use of the designed emulsion-based creams can safely enhance the delivery of the hydrolysed collagen in the skin layers with additional benefits for cosmetic and therapeutic applications.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100253"},"PeriodicalIF":5.1,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances and innovative applications of persian gum as a native hydrocolloid: Physicochemical properties, biopolymer compatibility, and encapsulation technologies 作为天然水胶体的波斯胶的进展和创新应用：物理化学性质、生物聚合物相容性和封装技术

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-10-24 DOI: 10.1016/j.fhfh.2025.100250

Zahra Khoshdouni Farahani , Mohammad ali Ebrahimzadeh Mousavi , Abdorreza Mohammadi Nafchi

This mini review focuses on Persian gum, a native Iranian hydrocolloid derived from the mountain almond tree (Amygdalus species), which has attracted significant interest due to its unique structural properties and potential applications across various industries, especially in food and pharmaceuticals. Despite its promising characteristics, the use of Persian gum in coating and encapsulation technologies within the food industry remains limited. This paper highlights recent advances in the development of Persian gum-based beads and capsules for encapsulation purposes. Utilizing this native gum as a cost-effective coating material not only offers a sustainable alternative to expensive imported hydrocolloids but also provides comparable quality in food formulations. Moreover, Persian gum demonstrates excellent compatibility with other biopolymers and proteins, broadening its potential applications. The review summarizes experimental findings on Persian gum’s encapsulation efficiency, interactions with other gums and proteins, and its role in various coating methods. These insights underscore Persian gum’s promising role as a versatile and economical encapsulating agent, with significant potential for broader industrial application.

这篇综述主要介绍了波斯胶，一种源自山杏仁树的伊朗原生水胶体，由于其独特的结构特性和在各个行业，特别是食品和制药领域的潜在应用，引起了人们的极大兴趣。尽管具有很好的特性，波斯胶在食品工业的涂层和封装技术中的应用仍然有限。本文重点介绍了用于封装目的的波斯胶基珠和胶囊的最新进展。利用这种天然胶作为一种具有成本效益的涂层材料，不仅为昂贵的进口水胶体提供了可持续的替代品，而且在食品配方中也提供了相当的质量。此外，波斯胶与其他生物聚合物和蛋白质具有良好的相容性，扩大了其潜在的应用范围。综述了波斯胶的包封效率、与其它树胶和蛋白质的相互作用以及在各种包封方法中的作用。这些见解强调了波斯胶作为一种多功能和经济的包封剂的前景，具有更广泛的工业应用潜力。

{"title":"Advances and innovative applications of persian gum as a native hydrocolloid: Physicochemical properties, biopolymer compatibility, and encapsulation technologies","authors":"Zahra Khoshdouni Farahani , Mohammad ali Ebrahimzadeh Mousavi , Abdorreza Mohammadi Nafchi","doi":"10.1016/j.fhfh.2025.100250","DOIUrl":"10.1016/j.fhfh.2025.100250","url":null,"abstract":"<div><div>This mini review focuses on Persian gum, a native Iranian hydrocolloid derived from the mountain almond tree (<em>Amygdalus</em> species), which has attracted significant interest due to its unique structural properties and potential applications across various industries, especially in food and pharmaceuticals. Despite its promising characteristics, the use of Persian gum in coating and encapsulation technologies within the food industry remains limited. This paper highlights recent advances in the development of Persian gum-based beads and capsules for encapsulation purposes. Utilizing this native gum as a cost-effective coating material not only offers a sustainable alternative to expensive imported hydrocolloids but also provides comparable quality in food formulations. Moreover, Persian gum demonstrates excellent compatibility with other biopolymers and proteins, broadening its potential applications. The review summarizes experimental findings on Persian gum’s encapsulation efficiency, interactions with other gums and proteins, and its role in various coating methods. These insights underscore Persian gum’s promising role as a versatile and economical encapsulating agent, with significant potential for broader industrial application.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100250"},"PeriodicalIF":5.1,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in food-grade hydrogel encapsulation of probiotics with next-generation prebiotics for targeted synbiotic delivery 新一代益生元靶向合成给药的食品级水凝胶包封益生菌研究进展

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-10-23 DOI: 10.1016/j.fhfh.2025.100251

Vidmantė Minelgaitė, Sigita Jeznienė, Aušra Šipailienė

Background. Hydrogel-based encapsulation represents a successful method for preserving probiotics and their functionality during processing, storage, and gastrointestinal transit. Although conventional systems offer protection, advances in next-generation prebiotics such as polyphenols and non-digestible carbohydrates have shifted the focus toward multifunctional synbiotic delivery systems. These materials provide sustainable, food-grade design and improve both structural integrity and bioactivity. Additionally, fabrication and crosslinking methods such as ionic interactions, Maillard conjugation, and enzymatic or pH-responsive techniques facilitate precise hydrogel customization for targeted probiotic release. Scope and approach. This review discusses recent progress in natural and composite hydrogels for probiotic encapsulation, with particular attention to the influence of next generation prebiotics on hydrogel functionality. A decision-oriented design framework is presented, aligning target sites and release triggers with materials and crosslinking strategies, exemplified with quantitative results. This framework offers a systematic approach for selecting food-grade matrices and encapsulation methods. Key findings and conclusions. Natural and composite hydrogels provide effective protection against oxygen, heat, acidity, bile salts, and digestive enzymes, maintaining a hydrated and biocompatible microenvironment. Protein–polysaccharide combinations increase mechanical and rheological stability, while next-generation prebiotics further reinforce structural integrity and bioactivity. Encapsulation efficiencies above 90 percent and enhanced cell viability in simulated digestion demonstrate promising performance of these systems. The integration of quantitative mapping and design principles establishes a practical framework for developing scalable, food-grade, multifunctional synbiotic hydrogels, supporting advancements in probiotic delivery technology. Adopting standardized digestion models and prioritizing in vivo validation will aid the development of synbiotic hydrogels acceptable in real food systems.

背景。水凝胶包封是一种在加工、储存和胃肠道运输过程中保存益生菌及其功能的成功方法。虽然传统的系统提供保护，新一代益生元如多酚和不可消化的碳水化合物的进步已经将重点转移到多功能合成输送系统。这些材料提供了可持续的食品级设计，并提高了结构完整性和生物活性。此外，制造和交联方法，如离子相互作用、美拉德偶联、酶或ph响应技术，有助于精确定制靶向益生菌释放的水凝胶。范围和方法。本文综述了用于益生菌包封的天然和复合水凝胶的最新进展，特别关注了下一代益生元对水凝胶功能的影响。提出了一个决策导向的设计框架，将目标地点和释放触发器与材料和交联策略对齐，并以定量结果为例。该框架为选择食品级基质和封装方法提供了系统的方法。主要发现和结论。天然和复合水凝胶提供有效的保护，防止氧气，热量，酸性，胆汁盐和消化酶，维持一个水合和生物相容性的微环境。蛋白质-多糖组合增加了机械和流变稳定性，而下一代益生元进一步增强了结构完整性和生物活性。90%以上的封装效率和在模拟消化中增强的细胞活力证明了这些系统的良好性能。定量制图和设计原则的整合为开发可扩展的食品级多功能合成水凝胶建立了一个实用框架，支持益生菌输送技术的进步。采用标准化的消化模型和优先进行体内验证将有助于开发在实际食物系统中可接受的合成水凝胶。

{"title":"Advances in food-grade hydrogel encapsulation of probiotics with next-generation prebiotics for targeted synbiotic delivery","authors":"Vidmantė Minelgaitė, Sigita Jeznienė, Aušra Šipailienė","doi":"10.1016/j.fhfh.2025.100251","DOIUrl":"10.1016/j.fhfh.2025.100251","url":null,"abstract":"<div><div>Background. Hydrogel-based encapsulation represents a successful method for preserving probiotics and their functionality during processing, storage, and gastrointestinal transit. Although conventional systems offer protection, advances in next-generation prebiotics such as polyphenols and non-digestible carbohydrates have shifted the focus toward multifunctional synbiotic delivery systems. These materials provide sustainable, food-grade design and improve both structural integrity and bioactivity. Additionally, fabrication and crosslinking methods such as ionic interactions, Maillard conjugation, and enzymatic or pH-responsive techniques facilitate precise hydrogel customization for targeted probiotic release. Scope and approach. This review discusses recent progress in natural and composite hydrogels for probiotic encapsulation, with particular attention to the influence of next generation prebiotics on hydrogel functionality. A decision-oriented design framework is presented, aligning target sites and release triggers with materials and crosslinking strategies, exemplified with quantitative results. This framework offers a systematic approach for selecting food-grade matrices and encapsulation methods. Key findings and conclusions. Natural and composite hydrogels provide effective protection against oxygen, heat, acidity, bile salts, and digestive enzymes, maintaining a hydrated and biocompatible microenvironment. Protein–polysaccharide combinations increase mechanical and rheological stability, while next-generation prebiotics further reinforce structural integrity and bioactivity. Encapsulation efficiencies above 90 percent and enhanced cell viability in simulated digestion demonstrate promising performance of these systems. The integration of quantitative mapping and design principles establishes a practical framework for developing scalable, food-grade, multifunctional synbiotic hydrogels, supporting advancements in probiotic delivery technology. Adopting standardized digestion models and prioritizing in vivo validation will aid the development of synbiotic hydrogels acceptable in real food systems.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100251"},"PeriodicalIF":5.1,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization and physicochemical properties of a novel microencapsulated bio-calcium from Asian sea bass bones 新型微囊生物钙的制备及理化性质研究

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-10-11 DOI: 10.1016/j.fhfh.2025.100247

Salinee Phengleng , Wipavadee Sangadkit , Songsak Wattanachaisaereekul , Jiraporn Sirison , Suneerat Ruangsomboon

Calcium is the most abundant mineral in the human body, yet intake remains insufficient in many populations. Fishbone-derived bio-calcium from Asian sea bass (Lates calcarifer), containing approximately 37.5 % calcium (dry weight), offers a cost-effective source. However, its primary form, hydroxyapatite, has low solubility due to high crystallinity, limiting its application in food fortification. This study aimed to enhance the physicochemical properties of bio-calcium (B) powders by encapsulating them with maltodextrin (M), gum arabic (G), and their combination (MG) at 5 %, 10 %, and 15 % (w/v) using spray drying. A 1:4 (w/w) ratio of B to wall materials was applied at 180 °C (inlet) and 60 °C (outlet) temperatures. Powder yields ranged from 25.2 % (15 % BG) to 30.3 % (15 % BM), with no significant differences (p > 0.05) among treatments. Encapsulated powders had higher lightness (L*) than B. The highest calcium content and encapsulation efficiency were observed in 5 % BG, while BM showed the lowest. Moisture content and water activity remained below 10 % and 0.6 %, respectively. BG had the highest hygroscopicity, while wall concentration had no significant (p > 0.05) impact. Encapsulation improved water solubility index (75.4–86.5 %), especially in BM. Particle sizes ranged from 0.92 µm (10 % BMG) to 2.89 µm (15 % BM), while zeta potentials ranged from -8.71 mV (15 % BM) to -20.90 mV (15 % BMG). Encapsulated powders were more spherical and smoother than B, while BG particles showed aggregation, whereas BMG showed mixed morphologies. These findings suggest that encapsulation enhanced the physicochemical properties of bio-calcium, supporting its potential application in calcium-fortified foods and dietary supplements.

钙是人体内最丰富的矿物质，但许多人的摄入量仍然不足。从亚洲黑鲈（Lates calcarifer）中提取的鱼骨生物钙含有约37.5%的钙（干重），是一种具有成本效益的来源。然而，其主要形式羟基磷灰石由于结晶度高，溶解度低，限制了其在食品强化中的应用。以5%、10%和15% （w/v）浓度的麦芽糖糊精(M)、阿拉伯树胶(G)及其组合物（MG）包封生物钙(B)粉体，采用喷雾干燥的方法提高生物钙(B)粉体的理化性能。在180°C（进口）和60°C（出口）温度下，B与壁材的比例为1:4 （w/w）。粗粉产量在25.2% (15% BG) ~ 30.3% （15% BM）之间，各处理间无显著差异（p > 0.05）。包封粉末的轻度（L*）比b高，5% BG的钙含量和包封效率最高，BM最低。水分含量和水活度分别保持在10%和0.6%以下。BG吸湿性最高，壁浓度无显著影响（p > 0.05）。包封提高了水溶性指数（75.4 - 86.5%），尤其是在BM中。粒径范围从0.92µm （10% BMG）到2.89µm (15% BMG)，而zeta电位范围从-8.71 mV （15% BMG）到-20.90 mV （15% BMG）。包封后的粉末比B更圆润、光滑，BG颗粒呈聚集状，BMG颗粒呈混合状。这些发现表明，包封增强了生物钙的物理化学性质，支持其在钙强化食品和膳食补充剂中的潜在应用。

{"title":"Characterization and physicochemical properties of a novel microencapsulated bio-calcium from Asian sea bass bones","authors":"Salinee Phengleng , Wipavadee Sangadkit , Songsak Wattanachaisaereekul , Jiraporn Sirison , Suneerat Ruangsomboon","doi":"10.1016/j.fhfh.2025.100247","DOIUrl":"10.1016/j.fhfh.2025.100247","url":null,"abstract":"<div><div>Calcium is the most abundant mineral in the human body, yet intake remains insufficient in many populations. Fishbone-derived bio-calcium from Asian sea bass (<em>Lates calcarifer</em>), containing approximately 37.5 % calcium (dry weight), offers a cost-effective source. However, its primary form, hydroxyapatite, has low solubility due to high crystallinity, limiting its application in food fortification. This study aimed to enhance the physicochemical properties of bio-calcium (B) powders by encapsulating them with maltodextrin (M), gum arabic (G), and their combination (MG) at 5 %, 10 %, and 15 % (w/v) using spray drying. A 1:4 (w/w) ratio of B to wall materials was applied at 180 °C (inlet) and 60 °C (outlet) temperatures. Powder yields ranged from 25.2 % (15 % BG) to 30.3 % (15 % BM), with no significant differences (<em>p</em> > 0.05) among treatments. Encapsulated powders had higher lightness (L*) than B. The highest calcium content and encapsulation efficiency were observed in 5 % BG, while BM showed the lowest. Moisture content and water activity remained below 10 % and 0.6 %, respectively. BG had the highest hygroscopicity, while wall concentration had no significant (p > 0.05) impact. Encapsulation improved water solubility index (75.4–86.5 %), especially in BM. Particle sizes ranged from 0.92 µm (10 % BMG) to 2.89 µm (15 % BM), while zeta potentials ranged from -8.71 mV (15 % BM) to -20.90 mV (15 % BMG). Encapsulated powders were more spherical and smoother than B, while BG particles showed aggregation, whereas BMG showed mixed morphologies. These findings suggest that encapsulation enhanced the physicochemical properties of bio-calcium, supporting its potential application in calcium-fortified foods and dietary supplements.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100247"},"PeriodicalIF":5.1,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profiling oligosaccharide components in Polygonatum kingianum with potential anti-NAFLD activity using UPLC-Orbitrap-MS/MS technology 利用UPLC-Orbitrap-MS/MS技术分析黄精中具有潜在抗nafld活性的低聚糖成分

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-10-11 DOI: 10.1016/j.fhfh.2025.100248

Hong Guo , Rui Yao , Jing Fan , Ying Wang , Lingzhi Zhang , Hua Sun , Xiaohan Guo , Jianbo Yang , Jingzhe Pu , Yazhong Zhang , Baozhong Duan , Jia Chen , Wenguang Jing , Xianlong Cheng , Feng Wei

Polygonati Rhizoma serve as medicinal and edible plants rich in carbohydrates, with its polysaccharides extensively studied, while research on oligosaccharides remains limited regarding their structural and bioactive properties. This study investigates the against non-alcoholic fatty liver disease (NAFLD) effects of Polygonatum kingianum oligosaccharides (PKO) and employs UPLC-Orbitrap-MS/MS technology to characterize oligosaccharide components. The PKO components were enriched through hot water extraction, 60% anhydrous ethanol precipitation, and membrane separation technology, with preliminary validation of their hepatoprotective effects in DL-ethionine-induced fatty liver mice. A novel LC-MS/MS analytical method was developed utilizing characteristic secondary fragment ions and retention time profiles of oligosaccharides to enable rapid identification and characterization of PKO. Thirty-four oligosaccharides were successfully identified from Polygonatum kingianum (PK), all exhibiting a monosaccharide composition of fructose and glucose. The structural backbone consists of fructose residues linked via (1→2) and (2→6) glycosidic bonds, with 32 compounds being reported for the first time in PK. This study marks the first discovery of oligosaccharides in PK with potential therapeutic effects against NAFLD. Additionally, it represents the first application of LC-MS/MS technology to systematically characterize neutral oligosaccharides in this species. These findings provide a novel analytical methodology for investigating potential anti-NAFLD oligosaccharide components in PK.

黄精是一种富含碳水化合物的药用和食用植物，其多糖被广泛研究，而低聚糖的结构和生物活性研究仍然有限。本研究探讨了黄精低聚糖（PKO）抗非酒精性脂肪性肝病（NAFLD）的作用，并采用UPLC-Orbitrap-MS/MS技术对其成分进行了表征。通过热水提取、60%无水乙醇沉淀和膜分离技术对PKO成分进行富集，初步验证了其对dl -乙硫氨酸诱导的脂肪肝小鼠的肝保护作用。建立了一种新的LC-MS/MS分析方法，利用低聚糖的特征二级片段离子和保留时间谱来快速鉴定和表征PKO。从金黄精（Polygonatum kingianum， PK）中鉴定出34个低聚糖，均为果糖和葡萄糖的单糖组成。该结构骨架由果糖残基通过（1→2）和（2→6）糖苷键连接组成，其中32种化合物首次在PK中被报道。本研究标志着首次发现PK中具有潜在治疗NAFLD作用的低聚糖。此外，这是首次应用LC-MS/MS技术系统地表征该物种中的中性低聚糖。这些发现为研究PK中潜在的抗nafld低聚糖成分提供了一种新的分析方法。

{"title":"Profiling oligosaccharide components in Polygonatum kingianum with potential anti-NAFLD activity using UPLC-Orbitrap-MS/MS technology","authors":"Hong Guo , Rui Yao , Jing Fan , Ying Wang , Lingzhi Zhang , Hua Sun , Xiaohan Guo , Jianbo Yang , Jingzhe Pu , Yazhong Zhang , Baozhong Duan , Jia Chen , Wenguang Jing , Xianlong Cheng , Feng Wei","doi":"10.1016/j.fhfh.2025.100248","DOIUrl":"10.1016/j.fhfh.2025.100248","url":null,"abstract":"<div><div>Polygonati Rhizoma serve as medicinal and edible plants rich in carbohydrates, with its polysaccharides extensively studied, while research on oligosaccharides remains limited regarding their structural and bioactive properties. This study investigates the against non-alcoholic fatty liver disease (NAFLD) effects of <em>Polygonatum kingianum</em> oligosaccharides (PKO) and employs UPLC-Orbitrap-MS/MS technology to characterize oligosaccharide components. The PKO components were enriched through hot water extraction, 60% anhydrous ethanol precipitation, and membrane separation technology, with preliminary validation of their hepatoprotective effects in DL-ethionine-induced fatty liver mice. A novel LC-MS/MS analytical method was developed utilizing characteristic secondary fragment ions and retention time profiles of oligosaccharides to enable rapid identification and characterization of PKO. Thirty-four oligosaccharides were successfully identified from <em>Polygonatum kingianum</em> (PK), all exhibiting a monosaccharide composition of fructose and glucose. The structural backbone consists of fructose residues linked via (1→2) and (2→6) glycosidic bonds, with 32 compounds being reported for the first time in PK. This study marks the first discovery of oligosaccharides in PK with potential therapeutic effects against NAFLD. Additionally, it represents the first application of LC-MS/MS technology to systematically characterize neutral oligosaccharides in this species. These findings provide a novel analytical methodology for investigating potential anti-NAFLD oligosaccharide components in PK.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100248"},"PeriodicalIF":5.1,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145320223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Citrus flavonoid-pectin conjugates: Towards broad scope therapeutic agents 柑橘类黄酮-果胶缀合物：面向广泛用途的治疗剂

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-10-09 DOI: 10.1016/j.fhfh.2025.100246

Caterina Di Sano , Claudia D’Anna , Giovanna Li Petri , Giuseppe Angellotti , Francesco Meneguzzo , Rosaria Ciriminna , Mario Pagliaro

Obtained via synthetic organic chemistry, enzymatic, free radical or cavitation-based routes, citrus flavonoid-pectin conjugates are bioconjugates showing substantial bioactivity. Reviewing research achievements concerning the biological activity of citrus flavonoid-pectin conjugates since their recent introduction, this study suggests that the use of water-soluble and biocompatible citrus pectin to chemically bind and deliver citrus flavonoids offers a synergistic solution to the poor bioavailability of flavonoids that so far limited their uptake. Merging the powerful and broad scope bioactivity of citrus flavonoids with that of pectin, the approach is promising towards the development of new pharmaceutical and nutraceutical products for the treatment and prevention of numerous diseases.

柑橘类黄酮-果胶缀合物是一种生物偶联物，通过合成有机化学、酶促、自由基或基于空化的途径获得。本文综述了近年来有关柑橘类黄酮-果胶缀合物生物活性的研究成果，认为利用水溶性和生物相容性的柑橘果胶化学结合和递送柑橘类黄酮，可以协同解决目前柑橘类黄酮生物利用度差、限制其吸收的问题。将柑橘类黄酮的强大而广泛的生物活性与果胶的生物活性相结合，该方法有望开发新的药物和营养保健品，用于治疗和预防多种疾病。

引用次数: 0

Pea protein and p-coumaric acid conjugates: New evidence on in vitro digestion, modulation of gut microbiota, and anti-inflammatory activity 豌豆蛋白和对香豆酸缀合物：关于体外消化、肠道菌群调节和抗炎活性的新证据

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-28 DOI: 10.1016/j.fhfh.2025.100245

Xuxiao Gong , Jie Lin , Gwoncheol Park , Saurabh Kadyan , Quancai Sun , Ravinder Nagpal , Leqi Cui

This study aimed to investigate the gut health benefits and anti-inflammatory property of pea protein isolates (PPI) as affected by conjugating with p-coumaric acid (p-CA). ¹³C NMR analysis confirmed the newly formed covalent bonds between PPI and p-CA occurred at the C8 position of p-CA. Results showed that after in vitro digestion, the conjugates exhibited higher protein digestibility compared to PPI. Dialysis was conducted to simulate small intestinal absorption and obtain non-absorbable fraction, which were utilized for human fecal fermentation and for the evaluation of anti-inflammatory activity in RAW 264.7 cells. Gut microbiota analysis revealed that PPI reduced the abundance of Bifidobacterium and increased Lactobacillus. However, after conjugation with p-CA, the inhibitory effect of PPI on Bifidobacterium was diminished, while its promotive effect on Lactobacillus was further strengthened. Meanwhile, PPI exhibited anti-inflammatory potential, as evidenced by the significant suppression of nitric oxide (NO) production and IL-6 expression. While the conjugates induced higher NO production compared to PPI, the levels remained lower than those induced by LPS alone, suggesting a partial reduction in anti-inflammatory potential after conjugation. Overall, this study provides new evidence that conjugation with phenolics can enhance pea protein's potential to positively influence gut microbiota composition, albeit with a reduced anti-inflammatory potential.

本研究旨在探讨豌豆分离蛋白（PPI）与对香豆酸（p-CA）偶联对肠道健康和抗炎特性的影响。13C核磁共振分析证实PPI与p-CA之间新形成的共价键发生在p-CA的C8位置。结果表明，体外消化后，与PPI相比，缀合物具有更高的蛋白质消化率。透析模拟小肠吸收，获得不可吸收部分，用于人粪便发酵和评价RAW 264.7细胞的抗炎活性。肠道菌群分析显示，PPI降低了双歧杆菌的丰度，增加了乳酸杆菌的丰度。但与p-CA偶联后，PPI对双歧杆菌的抑制作用减弱，对乳酸菌的促进作用进一步增强。同时，PPI具有抗炎作用，可显著抑制一氧化氮（NO）的产生和IL-6的表达。虽然与PPI相比，偶联物诱导了更高的NO生成，但其水平仍低于LPS单独诱导的水平，这表明偶联物后抗炎潜力部分降低。总的来说，这项研究提供了新的证据，证明与酚类物质结合可以增强豌豆蛋白对肠道微生物群组成的积极影响，尽管抗炎能力降低。

{"title":"Pea protein and p-coumaric acid conjugates: New evidence on in vitro digestion, modulation of gut microbiota, and anti-inflammatory activity","authors":"Xuxiao Gong , Jie Lin , Gwoncheol Park , Saurabh Kadyan , Quancai Sun , Ravinder Nagpal , Leqi Cui","doi":"10.1016/j.fhfh.2025.100245","DOIUrl":"10.1016/j.fhfh.2025.100245","url":null,"abstract":"<div><div>This study aimed to investigate the gut health benefits and anti-inflammatory property of pea protein isolates (PPI) as affected by conjugating with p-coumaric acid (p-CA). <sup>13</sup>C NMR analysis confirmed the newly formed covalent bonds between PPI and p-CA occurred at the C8 position of p-CA. Results showed that after in vitro digestion, the conjugates exhibited higher protein digestibility compared to PPI. Dialysis was conducted to simulate small intestinal absorption and obtain non-absorbable fraction, which were utilized for human fecal fermentation and for the evaluation of anti-inflammatory activity in RAW 264.7 cells. Gut microbiota analysis revealed that PPI reduced the abundance of Bifidobacterium and increased Lactobacillus. However, after conjugation with p-CA, the inhibitory effect of PPI on Bifidobacterium was diminished, while its promotive effect on Lactobacillus was further strengthened. Meanwhile, PPI exhibited anti-inflammatory potential, as evidenced by the significant suppression of nitric oxide (NO) production and IL-6 expression. While the conjugates induced higher NO production compared to PPI, the levels remained lower than those induced by LPS alone, suggesting a partial reduction in anti-inflammatory potential after conjugation. Overall, this study provides new evidence that conjugation with phenolics can enhance pea protein's potential to positively influence gut microbiota composition, albeit with a reduced anti-inflammatory potential.</div></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"8 ","pages":"Article 100245"},"PeriodicalIF":5.1,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0