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The thirst-quenching capacity of carbonated thickened drinks 碳酸加厚饮料的解渴能力
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-06-13 DOI: 10.1016/j.fhfh.2025.100226
Ruth Picó-Munyoz, Amparo Tarrega, Laura Laguna
Thickened drinks can be prescribed to ensure the safety of individuals with swallowing difficulties. However, these beverages, especially those with starch-based thickeners, are often less appealing because of their texture and taste. Previous research has indicated that carbonation in water can improve beverage likeability and stimulate muscular activity, potentially helping in safer swallowing. This study investigates the potential of adding carbonation to thickened drinks to enhance oral hydration and alleviate dry mouth and thirst sensations. Nine different water samples prepared with or without a thickener (xanthan gum-based or starch-based) and with still or carbonated water (powder- sparkling water and sparkling commercial water) were studied. Oral moisture, dry mouth, and thirst-quenching sensations were measured before, immediately after, and 5 min after consumption. The two types of carbonation created resulted in different distribution of bubbles. Oral moisture was reduced after consuming thickened water. In addition, the thickened water, with or without carbonation, did not alleviate thirst or dry mouth. Despite the potential safety benefits of carbonation, it did not improve thirst or dry mouth sensations. Future research should explore other stimuli to enhance thirst-quenching capacity, like flavour addition to carbonation to enhance adherence to thickened beverages and body stimulation.
可以规定加厚饮料,以确保有吞咽困难的人的安全。然而,这些饮料,尤其是那些含有淀粉基增稠剂的饮料,由于它们的质地和味道,往往不那么吸引人。先前的研究表明,水中的碳酸可以提高饮料的亲和力,刺激肌肉活动,可能有助于更安全的吞咽。本研究探讨了在增稠饮料中添加碳酸以增强口服水合作用、缓解口干和口渴感觉的潜力。研究了添加或不添加增稠剂(黄原胶基或淀粉基)、无水或碳酸水(气泡水和泡沫商业水)制备的9种不同水样。在食用前、食用后和食用后5分钟分别测量口腔水分、口干和解渴感觉。这两种类型的碳酸化导致气泡的分布不同。饮用增稠水后,口腔水分减少。此外,加厚的水,无论是否加碳酸,都不能缓解口渴或口干。尽管碳酸饮料具有潜在的安全益处,但它并不能改善口渴或口干的感觉。未来的研究应该探索其他刺激来增强解渴能力,比如在碳酸饮料中添加风味来增强对增稠饮料的依从性和身体刺激。
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引用次数: 0
Role of oral probiotic supplementation on gut permeability in obesity: A systematic review of randomized controlled trials 口服益生菌补充剂对肥胖患者肠道通透性的作用:一项随机对照试验的系统综述
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-06-06 DOI: 10.1016/j.fhfh.2025.100225
Alpha Fardah Athiyyah , Triska Susila Nindya , Reza Gunadi Ranuh , Andy Darma , Khadijah Rizky Sumitro , Purwo Sri Rejeki , Lilik Djuari , Subijanto Marto Sudarmo , Rina Agustina

Background

Obesity is a chronic condition and a global epidemic, associated with impaired intestinal permeability owing to dysbiosis. Gut microbiota reportedly plays a potential role in obesity treatment. Probiotics have emerged as the most widely used approach for treating dysbiosis. This study explored the effect of oral probiotic supplementation on intestinal permeability in individuals with obesity.

Methods

A Systematic review was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses. PubMed, ScienceDirect, PLOS One, and Cochrane databases were searched to identify articles published between 2014 and September 2024. The Cochrane risk-of-bias tool for randomized trials was used to assess publication bias.

Results

A total of 690 human RCTs (randomized controlled trials) were identified, of which nine were eligible for this review. Probiotics (Akkermansia, Bifidobacterium, Enterococcus, Lactobacillus, Streptococcus, and a combination of Saccharomyces yeast) used for over 8 weeks may decrease gut permeability outcomes, including plasma zonulin, fecal zonulin, plasma or serum LPS, urinary lactulose, and urinary mannitol; however, BMI (body mass index) levels exhibited inconsistent measurements across all groups.

Conclusions

Probiotic supplementation for a minimum of 8 weeks may reduce gut permeability. However, further studies are required to develop standardized protocols for probiotic strains, dosages, durations, and delivery methods, to better understand their effects on intestinal permeability and related outcomes in individuals with obesity.
背景:肥胖是一种慢性疾病,是一种全球性的流行病,与生态失调引起的肠道通透性受损有关。据报道,肠道微生物群在肥胖治疗中发挥着潜在的作用。益生菌已成为治疗生态失调最广泛使用的方法。本研究探讨了口服益生菌补充剂对肥胖个体肠道通透性的影响。方法按照系统评价和meta分析的首选报告项目进行系统评价。检索了PubMed、ScienceDirect、PLOS One和Cochrane数据库,以确定2014年至2024年9月之间发表的文章。采用Cochrane随机试验风险偏倚工具评估发表偏倚。结果共纳入690项人类随机对照试验(rct),其中9项符合本综述。益生菌(阿克曼菌、双歧杆菌、肠球菌、乳酸杆菌、链球菌和酵母菌的组合)使用超过8周可能会降低肠道通透性结果,包括血浆带蛋白、粪便带蛋白、血浆或血清LPS、尿乳果糖和尿甘露醇;然而,BMI(身体质量指数)水平在所有组中显示出不一致的测量结果。结论补充益生菌至少8周可降低肠道通透性。然而,需要进一步的研究来制定益生菌菌株、剂量、持续时间和给药方法的标准化方案,以更好地了解它们对肥胖个体肠道通透性和相关结局的影响。
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引用次数: 0
Impact of partial enzymatic hydrolysis on the transport and uptake of SPI nanoparticles post-digestion 部分酶解对消化后SPI纳米颗粒运输和摄取的影响
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-06-01 DOI: 10.1016/j.fhfh.2025.100223
Ling Chen , Yuan Lv , Xiaoyong Wang , Maoshen Chen , Hui Zhang
Despite extensive research on protein-based nanoparticles for nutrient delivery, the structural features that govern bile salt interactions and subsequently enhance intestinal absorption and metabolic transformation remain insufficiently clarified. In this study, soy protein isolate nanoparticles enzymatically modified by Alcalase, Neutrase, and Flavorzyme (SPIH-A@NP, SPIHN@NP, SPIH-F@NP) were constructed as structural models to explore the relationship between nanoparticle interfacial properties, bile salt adsorption, and nutrient transport efficiency, using simulated gastrointestinal digestion and Caco-2/HT29 co-culture models. Compared with undigested nanoparticles and free β-carotene, digested nanoparticles exhibited significantly improved cellular uptake and transport efficiencies. Among them, SPIHN@NP and SPIH-F@NP retained more hydrophobic peptides and acidic subunits from 11S globulin, which enhanced bile salt adsorption, maintained smaller particle sizes post-digestion, and facilitated more efficient delivery of β-carotene and its metabolites. Additionally, the digestion process altered uptake pathways, with macropinocytosis contributing more prominently post-digestion. These findings provide mechanistic insight into structure–function relationships that can guide the rational design of protein-based nanocarriers for targeted absorption and metabolic modulation of lipophilic nutrients.
尽管基于蛋白质的纳米颗粒用于营养输送的研究广泛,但控制胆盐相互作用并随后增强肠道吸收和代谢转化的结构特征仍未得到充分阐明。本研究通过模拟胃肠道消化和Caco-2/HT29共培养模型,构建Alcalase、Neutrase和Flavorzyme (SPIH-A@NP, SPIHN@NP, SPIH-F@NP)酶修饰的大豆分离蛋白纳米颗粒作为结构模型,探讨纳米颗粒界面特性、胆盐吸附和营养物质运输效率之间的关系。与未消化的纳米颗粒和游离β-胡萝卜素相比,消化的纳米颗粒表现出显著提高的细胞摄取和运输效率。其中SPIHN@NP和SPIH-F@NP从11S球蛋白中保留了更多的疏水肽和酸性亚基,增强了胆盐的吸附,在消化后保持更小的粒径,促进了β-胡萝卜素及其代谢物的更有效递送。此外,消化过程改变了摄取途径,消化后巨噬细胞增多作用更为突出。这些发现提供了结构-功能关系的机制见解,可以指导基于蛋白质的纳米载体的合理设计,以靶向吸收和亲脂性营养物质的代谢调节。
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引用次数: 0
Synergistic anticancer enhancement: Sericin nanogels co-delivery of anthocyanin and cancer drugs irinotecan, paclitaxel, and oxaliplatin 协同抗癌增强:丝胶纳米凝胶共同递送花青素和抗癌药物伊立替康、紫杉醇和奥沙利铂
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-06-01 DOI: 10.1016/j.fhfh.2025.100224
Lei Zhang , Minglu Hao , Liang Yao , Linyuan Xue , Jingmou Yu , Dongming Xing , Pu Chen
In this study, we investigated the potential of a food derivative sericin nanogel-anthocyanin (SNG-C3G) nanocomposite to enhance the anticancer effects of various chemotherapeutic drugs, including irinotecan (IRI), paclitaxel (PTX), and oxaliplatin (OXA), on different cell lines such as HeLa, HSEC, and HEK293T. The SNG-C3G nanocomposites exhibited a uniform spherical morphology, and upon co-encapsulation with either IRI or PTX, the particle size and zeta potential remained relatively consistent, ranging from 24.96 to 29.44 nm and −18.37 to −23.67 mV, respectively. Our findings indicate a marked increase in the anticancer effectiveness when these drugs are combined with the SNG-C3G nanocomposite. Focusing on the interaction with PTX, our proteomic analysis revealed significant changes in cell behavior, including cytoprotective mechanisms, ligand interactions, stress response, and pathways associated with oxidative and metabolic detoxification. Specifically, 13 proteins were upregulated and 10 were downregulated in the SNG-C3G-PTX group compared to the PTX-only group. The strong antioxidant properties of C3G might stand out as a key factor in enhancing these effects. This study suggests the potential for developing new cancer treatment strategies that utilize anthocyanins to boost the effectiveness of traditional chemotherapy protocols.
在这项研究中,我们研究了食品衍生物丝胶纳米凝胶-花青素(SNG-C3G)纳米复合材料增强各种化疗药物(包括伊立替康(IRI)、紫杉醇(PTX)和奥沙利铂(OXA))对不同细胞系(如HeLa、HSEC和HEK293T)的抗癌作用的潜力。SNG-C3G纳米复合材料具有均匀的球形形貌,与IRI或PTX共包覆后,其粒径和zeta电位保持相对一致,分别在24.96 ~ 29.44 nm和- 18.37 ~ - 23.67 mV之间。我们的研究结果表明,当这些药物与SNG-C3G纳米复合材料联合使用时,抗癌效果显着增加。通过与PTX的相互作用,我们的蛋白质组学分析揭示了细胞行为的显著变化,包括细胞保护机制、配体相互作用、应激反应以及与氧化和代谢解毒相关的途径。具体而言,与仅ptx组相比,SNG-C3G-PTX组有13个蛋白上调,10个蛋白下调。C3G的强抗氧化性能可能是增强这些效果的关键因素。这项研究表明,开发新的癌症治疗策略的潜力,利用花青素来提高传统化疗方案的有效性。
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引用次数: 0
Development of a self-nanoemulsifying system for the oil extract of Mentha spicata L. and evaluation of its anticancer efficacy in vitro 薄荷油提取物自纳米乳化体系的建立及其体外抗癌效果评价
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-05-20 DOI: 10.1016/j.fhfh.2025.100222
F. Morovat , M. Karimi , R. Dehdari Vais , M. Negahdary , S.A. Dastgheib , H. Heli
Nanocarriers based on (natural) lipids have been extensively studied to improve the oral bioavailability of anticancer compounds/drugs with poor water solubility. Self-nanoemulsifying drug delivery systems (SNEDDSs) are a route to improve the solubility and bioavailability of lipophilic compounds. In a present study, an SNEDDS using the oil extract of Mentha spicata L. leaves, Tween 80, and PEG 600 (SMO) was prepared. Pseudo-ternary phase diagrams were constructed to find suitable surfactant, solubilizer with the oil extract that form self-nanoemulsion after dilution in water. SMO comprised Tween 80:PEG 600:the oil with 2:3:5 in volume ratios or 2.1:3.4:4.5 in mass ratios. It comprised nanodroplets of 82.5 ± 4.7 nm in diameter and had a stability of >45 days. SMO led to enhanced cytotoxicity of the oil extract with IC-50 values of 1.9 ± 0.1 and 2.0 ± 0.1 mg mL−1 for MDA-MB-231 and MCF-7 cells, respectively. SMO would be applicable for delivery of either water insoluble chemotherapeutic drugs or natural anticancer oils upon more investigations.
基于(天然)脂质的纳米载体已被广泛研究,以提高水溶性差的抗癌化合物/药物的口服生物利用度。自纳米乳化给药系统(snedds)是提高亲脂性化合物的溶解度和生物利用度的途径。本研究以薄荷叶油提取物、Tween 80和PEG 600 (SMO)为原料制备了SNEDDS。构建拟三元相图,寻找合适的表面活性剂、增溶剂和油提取物在水中稀释后形成自纳米乳。SMO由Tween 80: peg600:体积比为2:3:5或质量比为2.1:3.4:4.5的油组成。它由直径为82.5±4.7 nm的纳米液滴组成,稳定性为45天。SMO对MDA-MB-231和MCF-7细胞的IC-50值分别为1.9±0.1和2.0±0.1 mg mL−1,从而增强了油提取物的细胞毒性。在更多的研究中,SMO将适用于水不溶性化疗药物或天然抗癌油的输送。
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引用次数: 0
Optimization of phenolic extraction method and in vitro bioaccessibility of microencapsulated pigmented rice bran extracts and their antioxidant and anticancer properties 米糠微胶囊化色素提取物酚类提取方法、体外生物可及性及其抗氧化抗癌性能的优化
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-05-20 DOI: 10.1016/j.fhfh.2025.100221
Rhowell Navarro Tiozon Jr. , Glenn Vincent P. Ong , Kristel June D. Sartagoda , Sheba Mae M. Duque , Saleh Alseekh , Aldrin P. Bonto , Shem Gempesaw Jr. , Vipin Pratap , Florencio C. Reginio Jr. , Jonina Marie J. Tengco , Christian Seagan , Joel H G Tolentino , Dennis Marvin O. Santiago , Alisdair R. Fernie , Nese Sreenivasulu
Pigmented paddy rice is rich in a diverse array of phytochemicals that confer notable antioxidant and anticancer properties. However, the stability and bioaccessibility of these bioactive compounds present significant challenges. In this study, 542 brown (including pigmented and non-pigmented) whole grain rice samples were screened for their antioxidant components and capacity, leading to the identification of three superior cultivars: Balatinao variable purple rice, Ketan Hitam variable purple rice, and Kintuman red rice. Using response surface methodology, rice bran extracts from these cultivars were subjected to microencapsulation to stabilize the phytochemicals. Among the microencapsulated rice bran extracts (MRBEs), Ketan Hitam MRBE demonstrated significantly higher total phenolic content (TPC) and antioxidant capacity. This enhancement is likely due to the increased concentrations of isovitexin, luteolin 7-glucoside, and vitexin following microencapsulation. Furthermore, compared to non-encapsulated rice bran extracts, MRBEs exhibited significantly improved anticancer activity against HCT116 (colon) and A549 (lung) cancer cell lines (P < 0.05). Subsequent fractionation of the MRBE allowed for the identification of the most bioactive fractions, which contained metabolites effective against these cancer cell lines. In addition, in vitro bioaccessibility assays revealed a controlled release of 19 targeted phenolic compounds. This release profile was characterized by an initial increase during the gastric digestion phase, followed by a decrease in the intestinal phase. Notably, phenolic compounds such as chlorogenic acid, gallic acid, and vanillin were preserved across the three rice varieties after microencapsulation. These findings underscore the potential of MRBEs as functional food ingredients or supplements, offering improved bioaccessibility of phenolics, enhanced antioxidant properties, and promising anticancer activity. The results support the integration of rice bran extracts into the rice value chain, promoting their use in functional health applications.
色素水稻富含多种植物化学物质,具有显著的抗氧化和抗癌特性。然而,这些生物活性化合物的稳定性和生物可及性面临着重大挑战。本研究对542份糙米(包括色素和非色素)的抗氧化成分和抗氧化能力进行了筛选,鉴定出3个优良品种:Balatinao可变紫米、Ketan Hitam可变紫米和Kintuman红米。采用响应面法,对这些品种的米糠提取物进行微胶囊化处理,以稳定其化学成分。在微胶囊化米糠提取物(MRBEs)中,克坦希坦MRBE的总酚含量(TPC)和抗氧化能力显著提高。这种增强可能是由于微胶囊化后异牡荆素、木犀草素7-葡萄糖苷和牡荆素浓度的增加。此外,与未封装的米糠提取物相比,MRBEs对HCT116(结肠癌)和A549(肺癌)癌细胞的抗癌活性显著提高(P <;0.05)。随后对MRBE进行分离,鉴定出最具生物活性的部分,其中含有对这些癌细胞系有效的代谢物。此外,体外生物可及性测定显示19种目标酚类化合物的控释。这种释放曲线的特征是在胃消化阶段初始增加,随后在肠道阶段减少。值得注意的是,在微胶囊化后,绿原酸、没食子酸和香兰素等酚类化合物在三个水稻品种中都得到了保存。这些发现强调了MRBEs作为功能性食品成分或补充剂的潜力,可以改善酚类物质的生物可及性,增强抗氧化性能,并具有抗癌活性。研究结果支持将米糠提取物整合到稻米价值链中,促进其在功能性健康领域的应用。
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引用次数: 0
Development of sodium chloride microcapsules as saltiness enhancement strategy 氯化钠微胶囊增盐策略的研制
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-05-16 DOI: 10.1016/j.fhfh.2025.100219
Juan Carlos SOLOMANDO, Teresa ANTEQUERA, Abraham Pajuelo, Jorge RUIZ-CARRASCAL, Trinidad PEREZ-PALACIOS
This study aimed to develop salt microcapsules using different wall materials to achieve stable encapsulated salt systems with optimal water solubilization and controlled salt release in the oral cavity, leading to an acceptable saltiness perception in food matrices. Six types of salt microcapsules were prepared, uncoated salt (CONT) and coated with maltodextrin (MALT), chitosan (QUIT), double emulsion (DOBL), alginate (ALGI), and liposomes (LIPO).
Significant differences were observed in size distribution, intermolecular forces, solubility index, salt release during oral digestion, and sensory evaluation. MALT microcapsules exhibited the most homogeneous size distribution, while ALGI showed the smallest particle size (D(4.3) = 0.27 μm). QUIT and DOBL microcapsules demonstrated the strongest intermolecular interactions between Na+ and Cl− ions and their respective wall materials, giving the lowest solubility (77.95 and 58.74 %) and oral salt release values (39.51 and 45.87 %). Contrarily, CONT, MALT, ALGI, and LIPO microcapsules exhibited higher solubility (99.77, 90.33, 92.65 and 86.01 %) and salt release (96.62, 83.08, 88.22 and 86.01 %).
Sensory analysis revealed that coated salt microcapsules enhanced the perception of saltiness compared to uncoated samples, demonstrating the potential of salt encapsulation as a strategy to reduce overall sodium content in foods without compromising taste. However, QUIT and ALGI microcapsules received the lowest hedonic scores, possibly due to undesirable sensory attributes associated with their wall materials.
Overall, MALT, DOBL, and LIPO microcapsules emerged as the most promising formulations, offering a balance of favorable characteristics across multiple parameters and highlighting their potential application in the development of reduced-sodium food products.
本研究旨在利用不同的壁材开发盐微胶囊,以获得稳定的胶囊化盐系统,具有最佳的水溶性和控制盐在口腔中的释放,从而使食物基质具有可接受的咸味。制备了无包被盐(CONT)和包被麦芽糖糊精(MALT)、壳聚糖(QUIT)、双乳(DOBL)、海藻酸盐(ALGI)和脂质体(LIPO)的6种盐微胶囊。在大小分布、分子间力、溶解度指数、口腔消化时的盐释放和感官评价等方面观察到显著差异。MALT微胶囊粒径分布最均匀,ALGI微胶囊粒径最小(D(4.3) = 0.27 μm)。QUIT和DOBL微胶囊的Na+和Cl−离子与各自壁材的分子间相互作用最强,溶解度最低(77.95%和58.74%),口服盐释放值最低(39.51%和45.87%)。相反,CONT、MALT、ALGI和LIPO微胶囊的溶解度分别为99.77%、90.33%、92.65%和86.01%,盐释放度分别为96.62%、83.08、88.22%和86.01%。感官分析显示,与未包覆的样品相比,包覆盐微胶囊增强了对咸味的感知,这表明盐包覆作为一种降低食品中总钠含量而不影响味道的策略的潜力。然而,QUIT和ALGI微胶囊获得的享乐分数最低,可能是由于与壁材料相关的不良感官属性。总的来说,MALT、DOBL和LIPO微胶囊是最有前途的配方,在多个参数中提供了有利特性的平衡,并突出了它们在低钠食品开发中的潜在应用。
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引用次数: 0
Formulation optimization of orodispersible film containing essential oil from fruits of Zanthoxylum rhetsa (Roxb.) DC. using response surface methodology and evaluation of its antioxidant, and antiglycation activities 花椒果实精油光分散膜的配方优化直流。利用响应面法评价其抗氧化和抗糖化活性
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-05-16 DOI: 10.1016/j.fhfh.2025.100220
Pimpikar Kanchanadumkerng , Vilasinee Hirunpanich Sato , Nattawut Charoenthai , Thongtham Suksawat , Pattamapan Lomarat , Savita Chewchinda
This study aimed to develop an optimal formulation for an orodispersible film containing essential oil from Zanthoxylum rhetsa (ZR film) using response surface methodology with a Box-Behnken design experiment and to evaluate its antioxidant and antiglycation activities. Three independent variables—gelatin (1–3 % w/v), carrageenan (0.5–1.5 % w/v), and essential oil from Z. rhetsa fruits (1–3 % w/v)—were utilized to design a ZR film with desirable characteristics. Dependent variables, including film thickness, moisture content, and disintegration time, were assessed. The optimized formulation contained 1 % w/v gelatin, 0.5 % w/v carrageenan, and 2.6 % w/v essential oil from Z. rhetsa fruits. The optimized ZR film exhibited a thickness of 14.2 ± 2.12 µm, a disintegration time of 6.34 ± 0.53 min, and a moisture content of 3.32 ± 0.56 %. Total phenolic content was 55.3 ± 0.4 mg GAE/g, and ABTS radical scavenging capacity was 0.06 ± 0.02 g TEAC/g. Additionally, the ZR film inhibited the formation of advanced glycation end products (AGEs) by 66.97 ± 0.58 %. This ZR film shows promise as a potential oral healthcare product for managing oxidative stress and glycation-related pathologies.
采用Box-Behnken设计实验,采用响应面法优选花椒精油(ZR)光分散膜的最佳配方,并对其抗氧化和抗糖化活性进行评价。三个自变量-明胶(1 - 3% w/v),卡拉胶(0.5 - 1.5% w/v)和紫果精油(1 - 3% w/v) -被用来设计具有理想特性的ZR膜。因变量,包括薄膜厚度,水分含量和解体时间,进行了评估。优化后的配方含有1% w/v的明胶、0.5% w/v的卡拉胶、2.6% w/v的大黄果精油。优化后的ZR膜厚度为14.2±2.12µm,崩解时间为6.34±0.53 min,含水率为3.32±0.56%。总酚含量为55.3±0.4 mg GAE/g, ABTS自由基清除能力为0.06±0.02 g TEAC/g。ZR膜对晚期糖基化终产物(AGEs)的抑制率为66.97±0.58%。这种ZR薄膜显示了作为管理氧化应激和糖基化相关病理的潜在口腔保健产品的前景。
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引用次数: 0
Lactobacillus plantarum in high methoxylated pectin-based encapsulation systems supports gut epithelial wound healing in vitro 植物乳杆菌在高甲氧基果胶为基础的包封系统支持肠道上皮伤口愈合体外
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-05-13 DOI: 10.1016/j.fhfh.2025.100218
Felipe Galvez-Jiron , Lucas de Freitas Pedrosa , Luis Silva Lagos , Xin Tang , Traudy Wandersleben , Rodrigo Navia , Stephan Drusch , Francisca Acevedo , Paul de Vos
Wound healing and gut epithelial barrier regulation are crucial for intestinal homeostasis. Effective repair of gut epithelial wounds is crucial for re-establishing the mucosal barrier and resolving inflammation. Pectins, versatile polysaccharides in food, protect sensitive components, such as living bacteria, during gastrointestinal transit and support wound healing by promoting cell proliferation and migration.
This study investigated the effects of encapsulated Lactobacillus plantarum WCFS1 on cell proliferation and migration during epithelial wound healing, using pectin-based beads stabilized with alginate or chitosan. Pectin from lemon, with two degrees of methyl esterification (DM18 and DM88), produced four microbead types. T84 cells were incubated with these beads for 24 hours. The results revealed that cell proliferation and migration were influenced by the bead type, the degree of methyl esterification of the pectin, and the inclusion of L. plantarum. Empty DM88 pectin beads improved cell proliferation while reducing migration tendencies, whereas DM18 beads delayed migration.Beads containing L. plantarum demonstrated different effects: DM18 beads enhanced proliferation without inhibiting migration, while DM88 beads reduced migration. Both empty and L. plantarum-loaded beads decreased CCL20 production. Alginate/pectin beads with L. plantarum increased IL-8, while chitosan/pectin beads delayed migration, reduced CCL20 secretion, and modulated IL-8 levels.
In conclusion, DM88 pectin beads without L. plantarum and DM18 beads containing L. plantarum supported cell proliferation over migration during epithelial wound healing. These findings suggest that pectin-based encapsulation systems may play a role in promoting gut health, protecting barrier integrity, and delivering therapeutic agents.
伤口愈合和肠上皮屏障调节对肠道内稳态至关重要。肠道上皮伤口的有效修复对于重建粘膜屏障和消除炎症至关重要。果胶是食物中的多功能多糖,在胃肠道运输过程中保护敏感成分,如活细菌,并通过促进细胞增殖和迁移来支持伤口愈合。本研究利用海藻酸盐或壳聚糖稳定的果胶微球,研究了包封植物乳杆菌WCFS1对上皮伤口愈合过程中细胞增殖和迁移的影响。柠檬果胶经DM18和DM88两种程度的甲基酯化反应,可产生四种微珠。用这些珠培养T84细胞24小时。结果表明,细胞的增殖和迁移受珠粒类型、果胶的甲基酯化程度和植物乳杆菌包合物的影响。空DM88胶珠促进细胞增殖,降低迁移倾向,DM18胶珠则延迟迁移。含有植物乳杆菌的微球表现出不同的效果:DM18微球促进了植物乳杆菌的增殖,但不抑制迁移,DM88微球则抑制了迁移。空珠和载L. plantaram珠均降低CCL20产量。海藻酸盐/果胶珠可提高植物乳杆菌的IL-8水平,壳聚糖/果胶珠可延缓迁移,减少CCL20分泌,调节IL-8水平。综上所述,不含植物乳杆菌的DM88果胶珠和含有植物乳杆菌的DM18果胶珠在上皮伤口愈合过程中支持细胞增殖和迁移。这些发现表明,基于果胶的包封系统可能在促进肠道健康、保护屏障完整性和递送治疗剂方面发挥作用。
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引用次数: 0
Beneficial effects of soluble dietary fiber from Dendrocalamus brandisii Munro shoots on high-fat diet-induced metabolic disorders in mice: Impact on liver, adipose tissue, and intestinal health 布氏菖蒲芽可溶性膳食纤维对小鼠高脂肪饮食引起的代谢紊乱的有益作用:对肝脏、脂肪组织和肠道健康的影响
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2025-05-10 DOI: 10.1016/j.fhfh.2025.100217
Mingtian Tian , Jiahong Dong , Yuhong Guo , Yihe Zhao , Jianxin Cao
Bamboo shoot soluble dietary fiber (DBS-SDF) has numerous health-promoting benefits, but its protective effects and potential mechanism against obesity-related metabolic disorders remain unclear. The effects of DBS-SDF on metabolic disorders induced by a high-fat diet (HFD) in mice was studied. Supplementation with DBS-SDF alleviated the development of HFD-induced obesity and related symptoms by lowering serum lipid levels, preventing lipid degeneration, and modulating the expression levels of genes related to lipid synthesis and decomposition in the liver. At the same time, DBS-SDF reversed the enlargement of white adipose cells, prevented the whitening of brown adipose tissue, and activated fat browning to improve energy balance. In addition, DBS-SDF reshaped the structure of the gut microbiota, significantly reducing the ratio of Firmicutes/Bacteroidetes, promoting the proliferation of SCFA producing bacteria Ligilactobacillus, unclassified_Muribaculaceae, and Lachnospiraceae_NK4A136_group, while inhibiting harmful bacterial populations such as Faecalibaculum and Desulfovibrio. These effects are related to the enhancement of the intestinal barrier, such as upregulation of Intectin expression, increased secretion of mucin MUC2, and increased expression of tight junction proteins. Spearman correlation analysis revealed significant associations between obesity related indicators, intestinal barrier, and gut microbiota, emphasizing the improvement effect of DBS-SDF on metabolic disorders through a healthy gut microenvironment. These findings indicate that DBS-SDF has enormous potential in the development of prebiotics or functional foods.
竹笋可溶性膳食纤维(DBS-SDF)具有许多促进健康的益处,但其对肥胖相关代谢紊乱的保护作用和潜在机制尚不清楚。研究了DBS-SDF对小鼠高脂饮食引起的代谢紊乱的影响。补充DBS-SDF可通过降低血脂水平、防止脂质变性和调节肝脏中脂质合成和分解相关基因的表达水平,缓解hfd诱导的肥胖及相关症状的发生。同时,DBS-SDF逆转白色脂肪细胞增大,阻止棕色脂肪组织变白,激活脂肪褐变,改善能量平衡。此外,DBS-SDF重塑了肠道微生物群的结构,显著降低了厚壁菌门/拟杆菌门的比例,促进了产SCFA的细菌liilactobacillus、unclassified_Muribaculaceae和Lachnospiraceae_NK4A136_group的增殖,同时抑制了Faecalibaculum和Desulfovibrio等有害细菌群。这些作用与肠道屏障的增强有关,如Intectin表达上调、粘蛋白MUC2分泌增加、紧密连接蛋白表达增加等。Spearman相关分析显示肥胖相关指标、肠道屏障和肠道菌群之间存在显著相关性,强调DBS-SDF通过健康的肠道微环境改善代谢紊乱的作用。这些发现表明,DBS-SDF在开发益生元或功能食品方面具有巨大的潜力。
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Food Hydrocolloids for Health
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