Food Hydrocolloids for Health最新文献_第3页

Role of oral probiotic supplementation on gut permeability in obesity: A systematic review of randomized controlled trials 口服益生菌补充剂对肥胖患者肠道通透性的作用：一项随机对照试验的系统综述

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-06-06 DOI: 10.1016/j.fhfh.2025.100225

Alpha Fardah Athiyyah , Triska Susila Nindya , Reza Gunadi Ranuh , Andy Darma , Khadijah Rizky Sumitro , Purwo Sri Rejeki , Lilik Djuari , Subijanto Marto Sudarmo , Rina Agustina

Background

Obesity is a chronic condition and a global epidemic, associated with impaired intestinal permeability owing to dysbiosis. Gut microbiota reportedly plays a potential role in obesity treatment. Probiotics have emerged as the most widely used approach for treating dysbiosis. This study explored the effect of oral probiotic supplementation on intestinal permeability in individuals with obesity.

Methods

A Systematic review was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses. PubMed, ScienceDirect, PLOS One, and Cochrane databases were searched to identify articles published between 2014 and September 2024. The Cochrane risk-of-bias tool for randomized trials was used to assess publication bias.

Results

A total of 690 human RCTs (randomized controlled trials) were identified, of which nine were eligible for this review. Probiotics (Akkermansia, Bifidobacterium, Enterococcus, Lactobacillus, Streptococcus, and a combination of Saccharomyces yeast) used for over 8 weeks may decrease gut permeability outcomes, including plasma zonulin, fecal zonulin, plasma or serum LPS, urinary lactulose, and urinary mannitol; however, BMI (body mass index) levels exhibited inconsistent measurements across all groups.

Conclusions

Probiotic supplementation for a minimum of 8 weeks may reduce gut permeability. However, further studies are required to develop standardized protocols for probiotic strains, dosages, durations, and delivery methods, to better understand their effects on intestinal permeability and related outcomes in individuals with obesity.

背景：肥胖是一种慢性疾病，是一种全球性的流行病，与生态失调引起的肠道通透性受损有关。据报道，肠道微生物群在肥胖治疗中发挥着潜在的作用。益生菌已成为治疗生态失调最广泛使用的方法。本研究探讨了口服益生菌补充剂对肥胖个体肠道通透性的影响。方法按照系统评价和meta分析的首选报告项目进行系统评价。检索了PubMed、ScienceDirect、PLOS One和Cochrane数据库，以确定2014年至2024年9月之间发表的文章。采用Cochrane随机试验风险偏倚工具评估发表偏倚。结果共纳入690项人类随机对照试验（rct），其中9项符合本综述。益生菌（阿克曼菌、双歧杆菌、肠球菌、乳酸杆菌、链球菌和酵母菌的组合）使用超过8周可能会降低肠道通透性结果，包括血浆带蛋白、粪便带蛋白、血浆或血清LPS、尿乳果糖和尿甘露醇；然而，BMI（身体质量指数）水平在所有组中显示出不一致的测量结果。结论补充益生菌至少8周可降低肠道通透性。然而，需要进一步的研究来制定益生菌菌株、剂量、持续时间和给药方法的标准化方案，以更好地了解它们对肥胖个体肠道通透性和相关结局的影响。

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引用次数: 0

Enhanced oral delivery of zeaxanthin via chitosan/alginate nanoparticles: Optimizing stability and antioxidant efficacy in retinal cells 壳聚糖/海藻酸盐纳米颗粒增强玉米黄质口服递送：优化视网膜细胞的稳定性和抗氧化功效

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-11-10 DOI: 10.1016/j.fhfh.2025.100254

Feuangthit Niyamissara Sorasitthiyanukarn , Chawanphat Muangnoi , Nonthaneth Nalinratana , Pranee Rojsitthisak , Pornchai Rojsitthisak

Zeaxanthin (ZT), a lipophilic carotenoid with strong antioxidant potential, suffers from poor aqueous solubility and low oral bioavailability, which limits its therapeutic application. In this study, chitosan/alginate nanoparticles (CS/ALG-NPs) were developed and optimized for oral ZT delivery using a Box-Behnken design. The optimized NPs showed a particle size of 268 ± 35 nm, a zeta potential of −25.2 ± 0.8 mV, and an encapsulation efficiency of 75.4 ± 3.4%. In vitro release under simulated gastrointestinal (GI) conditions exhibited sustained release with improved digestive stability and bioaccessibility compared to free ZT. The optimized ZT-CS/ALG-NPs exhibited favorable stability, maintaining particle size, surface charge, and encapsulation efficiency during storage at 4 °C, and retained their spherical morphology and uniform dispersion after simulated GI digestion, supporting their potential for oral delivery applications. In ARPE-19 cells, ZT-CS/ALG-NPs significantly reduced intracellular reactive oxygen species, restored antioxidant enzyme activities (SOD, CAT, GPx), and increased intracellular glutathione (GSH) levels compared to free ZT. Under H₂O₂-induced oxidative stress, ZT-CS/ALG-NPs reduced the expression of pro-apoptotic proteins (Bax and cytochrome c) and increased the expression of anti-apoptotic protein (Bcl-2) in ARPE-19 cells (vs H₂O₂ group). Transmission electron microscopy and flow cytometry confirmed cellular uptake. These findings demonstrate the potential of CS/ALG-NPs as a polysaccharide-based oral delivery system to enhance the stability, bioaccessibility, and antioxidant efficacy of hydrophobic bioactives such as zeaxanthin.

玉米黄质（ZT）是一种亲脂性类胡萝卜素，具有较强的抗氧化潜力，但其水溶性差，口服生物利用度低，限制了其治疗应用。在这项研究中，壳聚糖/海藻酸盐纳米颗粒（CS/ALG-NPs）被开发和优化，用于口服ZT。优化后的NPs粒径为268±35 nm， zeta电位为−25.2±0.8 mV，包封效率为75.4±3.4%。与游离ZT相比，在模拟胃肠道（GI）条件下的体外释放表现出持续释放，并改善了消化稳定性和生物可及性。优化后的ZT-CS/ alg - np具有良好的稳定性，在4°C储存期间保持了粒径、表面电荷和包封效率，并且在模拟胃肠道消化后保持了其球形形态和均匀分散，支持其口服给药应用的潜力。在ARPE-19细胞中，与游离ZT相比，ZT- cs /ALG-NPs显著降低细胞内活性氧，恢复抗氧化酶活性（SOD、CAT、GPx），增加细胞内谷胱甘肽（GSH）水平。在H2O2诱导的氧化应激下，ZT-CS/ALG-NPs降低了ARPE-19细胞中促凋亡蛋白（Bax和细胞色素c）的表达，增加了抗凋亡蛋白（Bcl-2）的表达（与H2O2组相比）。透射电镜和流式细胞术证实细胞摄取。这些发现表明，CS/ALG-NPs作为一种基于多糖的口服给药系统，具有增强玉米黄质等疏水生物活性物质的稳定性、生物可及性和抗氧化功效的潜力。

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引用次数: 0

Advances in food-grade hydrogel encapsulation of probiotics with next-generation prebiotics for targeted synbiotic delivery 新一代益生元靶向合成给药的食品级水凝胶包封益生菌研究进展

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-10-23 DOI: 10.1016/j.fhfh.2025.100251

Vidmantė Minelgaitė, Sigita Jeznienė, Aušra Šipailienė

Background. Hydrogel-based encapsulation represents a successful method for preserving probiotics and their functionality during processing, storage, and gastrointestinal transit. Although conventional systems offer protection, advances in next-generation prebiotics such as polyphenols and non-digestible carbohydrates have shifted the focus toward multifunctional synbiotic delivery systems. These materials provide sustainable, food-grade design and improve both structural integrity and bioactivity. Additionally, fabrication and crosslinking methods such as ionic interactions, Maillard conjugation, and enzymatic or pH-responsive techniques facilitate precise hydrogel customization for targeted probiotic release. Scope and approach. This review discusses recent progress in natural and composite hydrogels for probiotic encapsulation, with particular attention to the influence of next generation prebiotics on hydrogel functionality. A decision-oriented design framework is presented, aligning target sites and release triggers with materials and crosslinking strategies, exemplified with quantitative results. This framework offers a systematic approach for selecting food-grade matrices and encapsulation methods. Key findings and conclusions. Natural and composite hydrogels provide effective protection against oxygen, heat, acidity, bile salts, and digestive enzymes, maintaining a hydrated and biocompatible microenvironment. Protein–polysaccharide combinations increase mechanical and rheological stability, while next-generation prebiotics further reinforce structural integrity and bioactivity. Encapsulation efficiencies above 90 percent and enhanced cell viability in simulated digestion demonstrate promising performance of these systems. The integration of quantitative mapping and design principles establishes a practical framework for developing scalable, food-grade, multifunctional synbiotic hydrogels, supporting advancements in probiotic delivery technology. Adopting standardized digestion models and prioritizing in vivo validation will aid the development of synbiotic hydrogels acceptable in real food systems.

背景。水凝胶包封是一种在加工、储存和胃肠道运输过程中保存益生菌及其功能的成功方法。虽然传统的系统提供保护，新一代益生元如多酚和不可消化的碳水化合物的进步已经将重点转移到多功能合成输送系统。这些材料提供了可持续的食品级设计，并提高了结构完整性和生物活性。此外，制造和交联方法，如离子相互作用、美拉德偶联、酶或ph响应技术，有助于精确定制靶向益生菌释放的水凝胶。范围和方法。本文综述了用于益生菌包封的天然和复合水凝胶的最新进展，特别关注了下一代益生元对水凝胶功能的影响。提出了一个决策导向的设计框架，将目标地点和释放触发器与材料和交联策略对齐，并以定量结果为例。该框架为选择食品级基质和封装方法提供了系统的方法。主要发现和结论。天然和复合水凝胶提供有效的保护，防止氧气，热量，酸性，胆汁盐和消化酶，维持一个水合和生物相容性的微环境。蛋白质-多糖组合增加了机械和流变稳定性，而下一代益生元进一步增强了结构完整性和生物活性。90%以上的封装效率和在模拟消化中增强的细胞活力证明了这些系统的良好性能。定量制图和设计原则的整合为开发可扩展的食品级多功能合成水凝胶建立了一个实用框架，支持益生菌输送技术的进步。采用标准化的消化模型和优先进行体内验证将有助于开发在实际食物系统中可接受的合成水凝胶。

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引用次数: 0

Extraction, purification, structure characterization of polysaccharides from Cistanche deserticola and their biological effects 肉苁蓉多糖的提取、纯化、结构表征及其生物学效应

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-07-30 DOI: 10.1016/j.fhfh.2025.100235

Faqin Tao , Baotang Zhao , Shen Song , Yunfei Xu , Ji Zhang

The polysaccharide fraction (CDP-D2-N1) from the stem of Cristanche deseticola was obtained after extraction and purification. The structural characteristics and in vitro antioxidant and anti-inflammatory activities of CDP-D2-N1 were investigated by physical, chemical and instrumental analyses. The results showed that CDP-D2-N1 (127.784 kDa) is a heterogeneous polysaccharide with an irregular non-crystalline structure, containing mainly Rha:Ara:Xyl:Gal:Gal:GlcA:Glc:GalA, and a molar ratio of 6.75 : 26.68 : 1.56 : 37.87 : 3.94 : 2.17 : 21.02. Combined with the results of NMR spectroscopy and methylation analyses, the main chain of CDP-D2-N1 was determined to be →4)-α-d-GalpA-(1→,→3,6)-β-d-Galp-(1→, →5)-α-l-Araf-(1→, containing a small amount of fragment →3,4)-β-d-Galp-(1→ and →3)-β-d-Galp-(1→, and the CDP-D2-N1 structure was inferred. In vitro assays demonstrated that CDP-D2-N1 possessed significant antioxidant capacity and attenuated inflammation induced by lipopolysaccharide (LPS) from Escherichia coli O55:B5 in bovine endothelial cells (bEEcs) by inhibiting the expression levels of TNF-α, IL-6, TLR 4 and IL-1.

提取纯化后，得到了地衣牡丹茎多糖部分（CDP-D2-N1）。采用物理、化学和仪器分析方法研究了CDP-D2-N1的结构特征及体外抗氧化和抗炎活性。结果表明，CDP-D2-N1 （127.784 kDa）是一种不规则的非晶体结构的多相多糖，主要成分为Rha:Ara:Xyl:Gal:Gal:GlcA:Glc:GalA，摩尔比为6.75:26.68:1.56:37.87:3.94:2.17:21.02。结合核磁共振波谱和甲基化分析结果，确定了CDP-D2-N1的主链为→4)-α-d- galpa -(1→，→3,6)-β-d- galp -(1→，→5)-α-l- araf -（1→），含有少量片段→3,4)-β-d- galp -(1→和→3)-β-d- galp -（1→），推断了CDP-D2-N1的结构。体外实验表明，CDP-D2-N1通过抑制TNF-α、IL-6、tlr4和IL-1的表达，具有显著的抗氧化能力，减轻了大肠杆菌O55:B5脂多糖（LPS）在牛内皮细胞（bEEcs）中的炎症反应。

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引用次数: 0

Green synthesis of biocompatible silver nanoparticles from Persicaria hydropiper leaves: Therapeutic and catalytic applications 绿色合成生物相容性银纳米粒子从水仙桃叶：治疗和催化应用

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-09-03 DOI: 10.1016/j.fhfh.2025.100237

Shajahan Talukder , Md. Shahin Ali , Sangita Kundu , Md. Abdul Aziz , Md. Rezaul Karim , Md. Habibur Rahman , M. Ahasanur Rabbi , Md. Rowshanul Habib

The study presents a sustainable method for synthesizing biocompatible silver nanoparticles from Persicaria hydropiper leaves (PH-AgNPs) with promising anti-inflammatory, antioxidant, anticancer, antimicrobial, and catalytic potentials. UV–vis analysis confirmed PH-AgNPs formation with a surface plasmon resonance (SPR) peak at 455 nm. FTIR spectra revealed the constituents of leaf extract involved in silver ion reduction. Characterization of PH-AgNPs was done using X-ray diffraction, dynamic light scattering, scanning electron microscopy, and energy dispersive X-ray techniques. This characterization confirmed that PH-AgNPs exhibited a crystalline, spherical shape with a strong silver peak. PH-AgNPs showed blood compatibility and a non-toxic nature as compared with Triton-X. PH-AgNPs exhibited moderate anti-inflammatory and antioxidant activities compared to diclofenac sodium and ascorbic acid. PH-AgNPs also showed potential as catalysts for the degradation of harmful dyes. In the anticancer experiment, PH-AgNPs treatments significantly (p < 0.05) decreased viable EAC (Ehrlich Ascites Carcinoma) cells and lowered weight gain in mice. PH-AgNPs (p < 0.05) restored blood counts and improved liver structure in treated mice versus untreated controls. Proapoptotic genes encoding p53 and Bax proteins were overexpressed in treated EAC cells, confirming the apoptotic hallmarks seen in fluorescence microscope images of these treated cells. PH-AgNPs demonstrated potent antibacterial activity, particularly against Shigella boydii, while erythromycin was the most effective against the same bacterium. PH-AgNPs combined with tetracycline showed the highest synergistic effect (37.26 %) against Escherichia coli. PH-AgNPs showed the lowest minimum inhibitory concentration (9.38 mg/mL) against Bacillus cereus. Therefore, the green-fabricated PH-AgNPs hold significant biological and environmental importance, offering potential for therapeutic and environmentally friendly applications.

该研究提出了一种可持续的方法来合成具有抗炎、抗氧化、抗癌、抗菌和催化潜力的生物相容性银纳米颗粒。紫外-可见分析证实了PH-AgNPs的形成，表面等离子体共振（SPR）峰在455nm处。FTIR光谱揭示了叶提取物参与银离子还原的成分。通过x射线衍射、动态光散射、扫描电子显微镜和能量色散x射线技术对PH-AgNPs进行了表征。这一表征证实了PH-AgNPs呈现出具有强银峰的结晶球形。与Triton-X相比，PH-AgNPs具有血液相容性和无毒性。与双氯芬酸钠和抗坏血酸相比，PH-AgNPs表现出中等的抗炎和抗氧化活性。PH-AgNPs还显示出作为降解有害染料催化剂的潜力。在抗癌实验中，PH-AgNPs处理显著（p < 0.05）降低了小鼠的EAC（埃利希腹水癌）活细胞，降低了小鼠的体重增加。PH-AgNPs （p < 0.05）恢复了治疗小鼠的血细胞计数，改善了肝脏结构。编码p53和Bax蛋白的促凋亡基因在处理过的EAC细胞中过度表达，证实了这些处理过的细胞的荧光显微镜图像中所见的凋亡特征。PH-AgNPs显示出强大的抗菌活性，特别是对博伊地志贺氏菌，而红霉素对同一细菌最有效。PH-AgNPs联合四环素对大肠杆菌的增效效果最高（37.26%）。PH-AgNPs对蜡样芽孢杆菌的最低抑菌浓度为9.38 mg/mL。因此，绿色制造的PH-AgNPs具有重要的生物学和环境意义，为治疗和环境友好应用提供了潜力。

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引用次数: 0

The thirst-quenching capacity of carbonated thickened drinks 碳酸加厚饮料的解渴能力

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-06-13 DOI: 10.1016/j.fhfh.2025.100226

Ruth Picó-Munyoz, Amparo Tarrega, Laura Laguna

Thickened drinks can be prescribed to ensure the safety of individuals with swallowing difficulties. However, these beverages, especially those with starch-based thickeners, are often less appealing because of their texture and taste. Previous research has indicated that carbonation in water can improve beverage likeability and stimulate muscular activity, potentially helping in safer swallowing. This study investigates the potential of adding carbonation to thickened drinks to enhance oral hydration and alleviate dry mouth and thirst sensations. Nine different water samples prepared with or without a thickener (xanthan gum-based or starch-based) and with still or carbonated water (powder- sparkling water and sparkling commercial water) were studied. Oral moisture, dry mouth, and thirst-quenching sensations were measured before, immediately after, and 5 min after consumption. The two types of carbonation created resulted in different distribution of bubbles. Oral moisture was reduced after consuming thickened water. In addition, the thickened water, with or without carbonation, did not alleviate thirst or dry mouth. Despite the potential safety benefits of carbonation, it did not improve thirst or dry mouth sensations. Future research should explore other stimuli to enhance thirst-quenching capacity, like flavour addition to carbonation to enhance adherence to thickened beverages and body stimulation.

可以规定加厚饮料，以确保有吞咽困难的人的安全。然而，这些饮料，尤其是那些含有淀粉基增稠剂的饮料，由于它们的质地和味道，往往不那么吸引人。先前的研究表明，水中的碳酸可以提高饮料的亲和力，刺激肌肉活动，可能有助于更安全的吞咽。本研究探讨了在增稠饮料中添加碳酸以增强口服水合作用、缓解口干和口渴感觉的潜力。研究了添加或不添加增稠剂（黄原胶基或淀粉基）、无水或碳酸水（气泡水和泡沫商业水）制备的9种不同水样。在食用前、食用后和食用后5分钟分别测量口腔水分、口干和解渴感觉。这两种类型的碳酸化导致气泡的分布不同。饮用增稠水后，口腔水分减少。此外，加厚的水，无论是否加碳酸，都不能缓解口渴或口干。尽管碳酸饮料具有潜在的安全益处，但它并不能改善口渴或口干的感觉。未来的研究应该探索其他刺激来增强解渴能力，比如在碳酸饮料中添加风味来增强对增稠饮料的依从性和身体刺激。

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引用次数: 0

Recent advances in plant protein microgels encapsulated with bioactive compounds 包封生物活性化合物的植物蛋白微凝胶研究进展

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-09-03 DOI: 10.1016/j.fhfh.2025.100238

Srutee Rout , Sena Bakir , Sonali Khanal , Gurjeet Kaur , Zakir Showkat Khan , Thameed Aijaz , Jyoti Sharma , Mohmad Sayeed Bhat , Farhana Mehraj , Ghazaleh Sadeghi Vahid , Dinesh Kumar , Prem Prakash Srivastav

Protein microgels and other novel food structuring methods have garnered attention to increase plant protein functionality. Microgels, small particles (0.5–5 μm) that trap a lot of solvent, are made of a three-dimensional network of cross-linked biopolymer molecules. Protein microgels exhibit structural adaptability that makes them excellent carriers for lipophilic bioactives, protecting them from degradation and instability in aqueous systems. Additionally, their tunable design allows controlled release, enhancing bioavailability and functional efficacy in food and nutraceutical applications. The integration of scientific advances with food technology positions plant protein-based microgels as transformative tools in developing sustainable, protein-rich, and functional plant-based food systems. These advances in protein microgels prompt this review of growing plant protein sources, microgel formation, stabilization technologies, encapsulation with bioactive compounds, bioactive component transport, focusing on their functional properties, structural features, and applications. Knowing these trends can help us understand how sustainable, functioning food systems will emerge and solve global environmental and nutritional challenges.

蛋白质微凝胶和其他新的食物结构方法已经引起了人们的关注，以增加植物蛋白的功能。微凝胶是一种小颗粒（0.5-5 μm），可捕获大量溶剂，由交联生物聚合物分子组成的三维网络构成。蛋白质微凝胶具有结构适应性，这使它们成为亲脂性生物活性的优良载体，保护它们免受降解和不稳定性。此外，其可调设计允许控制释放，提高食品和营养保健应用的生物利用度和功能功效。科学进步与食品技术的结合使植物蛋白微凝胶成为开发可持续、富含蛋白质和功能性植物性食品系统的变革性工具。这些蛋白质微凝胶的进展促使本文对生长中的植物蛋白来源、微凝胶的形成、稳定技术、生物活性化合物的包封、生物活性成分的运输、它们的功能特性、结构特征和应用进行综述。了解这些趋势可以帮助我们了解可持续运作的粮食系统将如何出现，并解决全球环境和营养挑战。

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引用次数: 0

Beta-glucan-enriched diets improve immune function, antioxidant activity, and survivability in challenged oysters 富含β -葡聚糖的饮食可以提高牡蛎的免疫功能、抗氧化活性和存活率

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-06-16 DOI: 10.1016/j.fhfh.2025.100227

Gustavo Waltzer Fehrenbach , João Rui Tanoeiro , Robert Pogue , Frank Carter , Marco F.L. Lemos , Patrick Murray , Declan Devine , Emanuele Rezoagli , Ian Major , Emma J. Murphy

Beta-glucans are bioactive polysaccharides with immunomodulatory and hydrocolloid properties. This study explores the potential of beta-glucans extracted from mushroom waste to enhance oyster (Crassostrea gigas) immunity and quality, providing a sustainable alternative to conventional aquaculture interventions. Beta-glucan supplementation led to a 100 % survival rate in treated groups compared to 70 % in the bacteria-only group (p = 0.024). Flow cytometry revealed a significant reduction in viable hemocytes in the beta-glucan group post-infection, suggesting modulation of inflammation. Protein content in gills, mantle, and digestive tissues increased by up to 2-fold in beta-glucan-fed oysters versus controls. Antioxidant activity, assessed by FRAP assay, was 25–40 % higher in the mantle and digestive tract if beta-glucan groups. Bacteriostatic assays demonstrated a marked reduction in Vibrio sp. Counts in tissues, particularly in the digestive tract, with bacterial load similar to uninfected controls. These findings highlight the dual functionality of beta-glucans as both immunomodulators and hydrocolloids, with implications for improving aquaculture sustainability and food safety. Further research should explore feeding behaviour, gut transit time, and nutrient absorption to optimize beta-glucan application in commercial shellfish production.

葡聚糖是具有免疫调节和水胶体特性的生物活性多糖。本研究探讨了从蘑菇废物中提取β -葡聚糖的潜力，以提高牡蛎（长牡蛎）的免疫力和质量，为传统水产养殖干预提供可持续的替代方案。补充β -葡聚糖导致治疗组的存活率为100%，而仅细菌组的存活率为70% （p = 0.024）。流式细胞术显示感染后β -葡聚糖组活血细胞显著减少，提示炎症调节。喂食β -葡聚糖的牡蛎的鳃、膜和消化组织中的蛋白质含量比对照组增加了2倍。经FRAP测定，β -葡聚糖组在肠膜和消化道的抗氧化活性高25 - 40%。抑菌试验显示，组织中，特别是消化道中的弧菌计数明显减少，细菌负荷与未感染的对照组相似。这些发现强调了β -葡聚糖作为免疫调节剂和水胶体的双重功能，对提高水产养殖的可持续性和食品安全具有重要意义。进一步的研究应探讨摄食行为、肠道转运时间和营养吸收，以优化β -葡聚糖在商业贝类生产中的应用。

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引用次数: 0

Profiling oligosaccharide components in Polygonatum kingianum with potential anti-NAFLD activity using UPLC-Orbitrap-MS/MS technology 利用UPLC-Orbitrap-MS/MS技术分析黄精中具有潜在抗nafld活性的低聚糖成分

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-10-11 DOI: 10.1016/j.fhfh.2025.100248

Hong Guo , Rui Yao , Jing Fan , Ying Wang , Lingzhi Zhang , Hua Sun , Xiaohan Guo , Jianbo Yang , Jingzhe Pu , Yazhong Zhang , Baozhong Duan , Jia Chen , Wenguang Jing , Xianlong Cheng , Feng Wei

Polygonati Rhizoma serve as medicinal and edible plants rich in carbohydrates, with its polysaccharides extensively studied, while research on oligosaccharides remains limited regarding their structural and bioactive properties. This study investigates the against non-alcoholic fatty liver disease (NAFLD) effects of Polygonatum kingianum oligosaccharides (PKO) and employs UPLC-Orbitrap-MS/MS technology to characterize oligosaccharide components. The PKO components were enriched through hot water extraction, 60% anhydrous ethanol precipitation, and membrane separation technology, with preliminary validation of their hepatoprotective effects in DL-ethionine-induced fatty liver mice. A novel LC-MS/MS analytical method was developed utilizing characteristic secondary fragment ions and retention time profiles of oligosaccharides to enable rapid identification and characterization of PKO. Thirty-four oligosaccharides were successfully identified from Polygonatum kingianum (PK), all exhibiting a monosaccharide composition of fructose and glucose. The structural backbone consists of fructose residues linked via (1→2) and (2→6) glycosidic bonds, with 32 compounds being reported for the first time in PK. This study marks the first discovery of oligosaccharides in PK with potential therapeutic effects against NAFLD. Additionally, it represents the first application of LC-MS/MS technology to systematically characterize neutral oligosaccharides in this species. These findings provide a novel analytical methodology for investigating potential anti-NAFLD oligosaccharide components in PK.

黄精是一种富含碳水化合物的药用和食用植物，其多糖被广泛研究，而低聚糖的结构和生物活性研究仍然有限。本研究探讨了黄精低聚糖（PKO）抗非酒精性脂肪性肝病（NAFLD）的作用，并采用UPLC-Orbitrap-MS/MS技术对其成分进行了表征。通过热水提取、60%无水乙醇沉淀和膜分离技术对PKO成分进行富集，初步验证了其对dl -乙硫氨酸诱导的脂肪肝小鼠的肝保护作用。建立了一种新的LC-MS/MS分析方法，利用低聚糖的特征二级片段离子和保留时间谱来快速鉴定和表征PKO。从金黄精（Polygonatum kingianum， PK）中鉴定出34个低聚糖，均为果糖和葡萄糖的单糖组成。该结构骨架由果糖残基通过（1→2）和（2→6）糖苷键连接组成，其中32种化合物首次在PK中被报道。本研究标志着首次发现PK中具有潜在治疗NAFLD作用的低聚糖。此外，这是首次应用LC-MS/MS技术系统地表征该物种中的中性低聚糖。这些发现为研究PK中潜在的抗nafld低聚糖成分提供了一种新的分析方法。

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引用次数: 0

Encapsulation of bifidobacteria in natural biopolymers: Advances, challenges, and applications in dairy products 双歧杆菌在天然生物聚合物中的包封：进展、挑战和在乳制品中的应用

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-12-01 Epub Date: 2025-11-04 DOI: 10.1016/j.fhfh.2025.100252

Marko Vinceković , Lana Živković , Botagoz Mutaliyeva , Galiya Madybekova , Elmira Turkeyeva , Nenad Jalšenjak , Assem Issayeva

Encapsulation of Bifidobacteria with natural biopolymers has emerged as a promising strategy to enhance the viability of probiotics in dairy systems. While Bifidobacteria confer well-established health benefits, their industrial application is hindered by sensitivity to processing and storage conditions. This review critically evaluates encapsulation methods, including extrusion, emulsification, spray drying, and freeze drying, as well as natural polymers such as alginate, chitosan, carrageenan, gelatin, and starch, with a focus on their ability to maintain the stability of probiotics. Beyond summarizing current techniques, we highlight persisting gaps in scalability, cost efficiency, and consumer acceptance, issues often overlooked in earlier reviews. By integrating recent advances in material science with food engineering perspectives, this article outlines pathways for industrial translation. It distinguishes itself by focusing on how encapsulation strategies can be optimized to overcome these barriers, ultimately enabling broader adoption of probiotic-enriched dairy products.

用天然生物聚合物封装双歧杆菌已成为一种有前途的策略，以提高益生菌在乳制品系统中的生存能力。虽然双歧杆菌具有公认的健康益处，但它们对加工和储存条件的敏感性阻碍了它们的工业应用。本文综述了包括挤压、乳化、喷雾干燥和冷冻干燥在内的封装方法，以及海藻酸盐、壳聚糖、卡拉胶、明胶和淀粉等天然聚合物，重点介绍了它们保持益生菌稳定性的能力。除了总结当前的技术之外，我们还强调了在可伸缩性、成本效率和消费者接受度方面持续存在的差距，这些问题在早期的评论中经常被忽视。通过将材料科学的最新进展与食品工程观点相结合，本文概述了工业翻译的途径。它的特点是专注于如何优化封装策略来克服这些障碍，最终使益生菌丰富的乳制品得到更广泛的采用。

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引用次数: 0