Food Hydrocolloids for Health最新文献_第3页

Profiling oligosaccharide components in Polygonatum kingianum with potential anti-NAFLD activity using UPLC-Orbitrap-MS/MS technology 利用UPLC-Orbitrap-MS/MS技术分析黄精中具有潜在抗nafld活性的低聚糖成分

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-10-11 DOI: 10.1016/j.fhfh.2025.100248

Hong Guo , Rui Yao , Jing Fan , Ying Wang , Lingzhi Zhang , Hua Sun , Xiaohan Guo , Jianbo Yang , Jingzhe Pu , Yazhong Zhang , Baozhong Duan , Jia Chen , Wenguang Jing , Xianlong Cheng , Feng Wei

Polygonati Rhizoma serve as medicinal and edible plants rich in carbohydrates, with its polysaccharides extensively studied, while research on oligosaccharides remains limited regarding their structural and bioactive properties. This study investigates the against non-alcoholic fatty liver disease (NAFLD) effects of Polygonatum kingianum oligosaccharides (PKO) and employs UPLC-Orbitrap-MS/MS technology to characterize oligosaccharide components. The PKO components were enriched through hot water extraction, 60% anhydrous ethanol precipitation, and membrane separation technology, with preliminary validation of their hepatoprotective effects in DL-ethionine-induced fatty liver mice. A novel LC-MS/MS analytical method was developed utilizing characteristic secondary fragment ions and retention time profiles of oligosaccharides to enable rapid identification and characterization of PKO. Thirty-four oligosaccharides were successfully identified from Polygonatum kingianum (PK), all exhibiting a monosaccharide composition of fructose and glucose. The structural backbone consists of fructose residues linked via (1→2) and (2→6) glycosidic bonds, with 32 compounds being reported for the first time in PK. This study marks the first discovery of oligosaccharides in PK with potential therapeutic effects against NAFLD. Additionally, it represents the first application of LC-MS/MS technology to systematically characterize neutral oligosaccharides in this species. These findings provide a novel analytical methodology for investigating potential anti-NAFLD oligosaccharide components in PK.

黄精是一种富含碳水化合物的药用和食用植物，其多糖被广泛研究，而低聚糖的结构和生物活性研究仍然有限。本研究探讨了黄精低聚糖（PKO）抗非酒精性脂肪性肝病（NAFLD）的作用，并采用UPLC-Orbitrap-MS/MS技术对其成分进行了表征。通过热水提取、60%无水乙醇沉淀和膜分离技术对PKO成分进行富集，初步验证了其对dl -乙硫氨酸诱导的脂肪肝小鼠的肝保护作用。建立了一种新的LC-MS/MS分析方法，利用低聚糖的特征二级片段离子和保留时间谱来快速鉴定和表征PKO。从金黄精（Polygonatum kingianum， PK）中鉴定出34个低聚糖，均为果糖和葡萄糖的单糖组成。该结构骨架由果糖残基通过（1→2）和（2→6）糖苷键连接组成，其中32种化合物首次在PK中被报道。本研究标志着首次发现PK中具有潜在治疗NAFLD作用的低聚糖。此外，这是首次应用LC-MS/MS技术系统地表征该物种中的中性低聚糖。这些发现为研究PK中潜在的抗nafld低聚糖成分提供了一种新的分析方法。

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引用次数: 0

Citrus flavonoid-pectin conjugates: Towards broad scope therapeutic agents 柑橘类黄酮-果胶缀合物：面向广泛用途的治疗剂

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-10-09 DOI: 10.1016/j.fhfh.2025.100246

Caterina Di Sano , Claudia D’Anna , Giovanna Li Petri , Giuseppe Angellotti , Francesco Meneguzzo , Rosaria Ciriminna , Mario Pagliaro

Obtained via synthetic organic chemistry, enzymatic, free radical or cavitation-based routes, citrus flavonoid-pectin conjugates are bioconjugates showing substantial bioactivity. Reviewing research achievements concerning the biological activity of citrus flavonoid-pectin conjugates since their recent introduction, this study suggests that the use of water-soluble and biocompatible citrus pectin to chemically bind and deliver citrus flavonoids offers a synergistic solution to the poor bioavailability of flavonoids that so far limited their uptake. Merging the powerful and broad scope bioactivity of citrus flavonoids with that of pectin, the approach is promising towards the development of new pharmaceutical and nutraceutical products for the treatment and prevention of numerous diseases.

柑橘类黄酮-果胶缀合物是一种生物偶联物，通过合成有机化学、酶促、自由基或基于空化的途径获得。本文综述了近年来有关柑橘类黄酮-果胶缀合物生物活性的研究成果，认为利用水溶性和生物相容性的柑橘果胶化学结合和递送柑橘类黄酮，可以协同解决目前柑橘类黄酮生物利用度差、限制其吸收的问题。将柑橘类黄酮的强大而广泛的生物活性与果胶的生物活性相结合，该方法有望开发新的药物和营养保健品，用于治疗和预防多种疾病。

引用次数: 0

Pea protein and p-coumaric acid conjugates: New evidence on in vitro digestion, modulation of gut microbiota, and anti-inflammatory activity 豌豆蛋白和对香豆酸缀合物：关于体外消化、肠道菌群调节和抗炎活性的新证据

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-28 DOI: 10.1016/j.fhfh.2025.100245

Xuxiao Gong , Jie Lin , Gwoncheol Park , Saurabh Kadyan , Quancai Sun , Ravinder Nagpal , Leqi Cui

This study aimed to investigate the gut health benefits and anti-inflammatory property of pea protein isolates (PPI) as affected by conjugating with p-coumaric acid (p-CA). ¹³C NMR analysis confirmed the newly formed covalent bonds between PPI and p-CA occurred at the C8 position of p-CA. Results showed that after in vitro digestion, the conjugates exhibited higher protein digestibility compared to PPI. Dialysis was conducted to simulate small intestinal absorption and obtain non-absorbable fraction, which were utilized for human fecal fermentation and for the evaluation of anti-inflammatory activity in RAW 264.7 cells. Gut microbiota analysis revealed that PPI reduced the abundance of Bifidobacterium and increased Lactobacillus. However, after conjugation with p-CA, the inhibitory effect of PPI on Bifidobacterium was diminished, while its promotive effect on Lactobacillus was further strengthened. Meanwhile, PPI exhibited anti-inflammatory potential, as evidenced by the significant suppression of nitric oxide (NO) production and IL-6 expression. While the conjugates induced higher NO production compared to PPI, the levels remained lower than those induced by LPS alone, suggesting a partial reduction in anti-inflammatory potential after conjugation. Overall, this study provides new evidence that conjugation with phenolics can enhance pea protein's potential to positively influence gut microbiota composition, albeit with a reduced anti-inflammatory potential.

本研究旨在探讨豌豆分离蛋白（PPI）与对香豆酸（p-CA）偶联对肠道健康和抗炎特性的影响。13C核磁共振分析证实PPI与p-CA之间新形成的共价键发生在p-CA的C8位置。结果表明，体外消化后，与PPI相比，缀合物具有更高的蛋白质消化率。透析模拟小肠吸收，获得不可吸收部分，用于人粪便发酵和评价RAW 264.7细胞的抗炎活性。肠道菌群分析显示，PPI降低了双歧杆菌的丰度，增加了乳酸杆菌的丰度。但与p-CA偶联后，PPI对双歧杆菌的抑制作用减弱，对乳酸菌的促进作用进一步增强。同时，PPI具有抗炎作用，可显著抑制一氧化氮（NO）的产生和IL-6的表达。虽然与PPI相比，偶联物诱导了更高的NO生成，但其水平仍低于LPS单独诱导的水平，这表明偶联物后抗炎潜力部分降低。总的来说，这项研究提供了新的证据，证明与酚类物质结合可以增强豌豆蛋白对肠道微生物群组成的积极影响，尽管抗炎能力降低。

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引用次数: 0

Formulation design of quercetin-loaded polyvinyl alcohol nanofibres for ocular drug delivery 槲皮素负载聚乙烯醇纳米纤维眼部给药的配方设计

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-24 DOI: 10.1016/j.fhfh.2025.100244

Rabiah Bashir , Adil Gani , Asima Shah , Hasham Shafi , Abdul Aala Fazili , Tabasum Ali , Syed Naiem Raza , Shabnam Kawoosa , Nisar Ahmad Khan

In the realm of ocular drug delivery, the limited bioavailability of pharmaceuticals presents a significant challenge. The development of alternative drug delivery systems aimed at enhancing the availability of medications at targeted sites is of considerable importance in addressing this issue. The research work aimed to design, formulate, and optimize a fast-dissolving nanofiber of quercetin and polyvinyl alcohol for an ocular delivery system. Response Surface Methodology (Box-Behnken design) was used for the optimisation of electrospinning parameters, including polymer concentration, flow rate, and voltage, resulting in enhanced drug encapsulation and the formation of smooth and uniform nanofibers. Morphological characterization was conducted using scanning electron microscopy, which demonstrated the development of smooth, uniform, bead-like porous fibers with the drug incorporated within the fibers in nanoform during the electrospinning process, rather than being deposited on the surface. The drug-excipient interaction was evaluated by FTIR analysis, which indicated compatibility between PVA and quercetin, with all the peaks of the quercetin retained in the optimized formulation. The conversion of quercetin from its crystalline form to an amorphous state was demonstrated through X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analyses. The XRD pattern illustrated a reduction in the crystalline peaks characteristic of quercetin, while the DSC results indicated a decrease in the melting point of quercetin in nanofibers. The physical, chemical, and pharmaceutical characteristics of the optimized nanofibre formulation were appropriate and within limits. Disintegration was accomplished within 45 s, with approximately 100% of the active ingredient released within 10 min during an in vitro dissolution test. In contrast, the conventional eye drop formulation exhibited a release of only 50%. Eye irritation study results indicated that there was no visual sign of irritation in all experimental rabbits. The optimized formulation underwent an accelerated stability study and retained its original properties with negligible changes. There was no significant alteration in physical appearance, flexibility, disintegration time, or drug encapsulation, confirming its stability.

在眼部给药领域，药物有限的生物利用度提出了一个重大挑战。在解决这一问题方面，开发旨在提高目标地点药物可得性的替代药物输送系统具有相当重要的意义。这项研究工作旨在设计、配制和优化一种用于眼部输送系统的速溶槲皮素和聚乙烯醇纳米纤维。采用响应面法（Box-Behnken设计）优化静电纺丝参数，包括聚合物浓度、流速和电压，从而增强药物包封性，形成光滑均匀的纳米纤维。利用扫描电镜进行了形态表征，结果表明，在静电纺丝过程中，药物以纳米形式融入纤维中，而不是沉积在表面，形成了光滑、均匀、珠状的多孔纤维。FTIR分析结果表明，PVA与槲皮素具有良好的配伍性，并保留了槲皮素的所有峰。通过x射线衍射（XRD）和差示扫描量热法（DSC）分析证实了槲皮素从晶体形态转变为非晶态。XRD分析表明槲皮素的结晶峰减少，DSC分析表明槲皮素在纳米纤维中的熔点降低。优化后的纳米纤维配方的物理、化学和药物特性是适当的，并且在限制范围内。在体外溶出试验中，崩解在45秒内完成，大约100%的活性成分在10分钟内释放。相比之下，传统滴眼液配方的释放率仅为50%。眼睛刺激研究结果表明，所有实验家兔均无视觉刺激迹象。优化后的配方进行了加速稳定性研究，并保持了其原有的性质，变化很小。在物理外观、柔韧性、崩解时间、药物包被等方面均无明显变化，证实了其稳定性。

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引用次数: 0

Smart-responsive chitosan dressings: From microenvironmental sensing to multifunctional precision wound healing 智能反应壳聚糖敷料：从微环境传感到多功能精确伤口愈合

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-20 DOI: 10.1016/j.fhfh.2025.100243

Minghui Sun , Yibo Zhang , Mingwei Zhou

Wound healing constitutes a multifaceted and dynamically regulated process that is modulated by a variety of microenvironmental factors such as pH, enzymes, and reactive oxygen species (ROS). Conventional wound dressings frequently fail to deliver the precise interventions necessary for the effective management of complex wounds. Chitosan, characterized by its high biocompatibility, biodegradability, and structural adaptability, exhibits enhanced self-healing photoactivity, rendering it an ideal candidate for the development of smart, responsive wound dressings. This review systematically examines the design strategies, molecular mechanisms, and therapeutic applications of chitosan-based smart dressings. It emphasizes their roles in infection control, inflammation reduction, angiogenesis, and tissue regeneration. Furthermore, we underscore the importance of multimodal systems incorporating adaptive feedback mechanisms (pH/ROS combinations, pH/enzyme and ROS/photothermal coupling) and explore their clinical potential in the treatment of diabetic foot ulcers, pressure ulcers, and other complex skin injuries. These findings provide a detailed framework for the development of the next generation of bio-responsive materials, fostering the integration of materials science with personalized wound care strategies.

伤口愈合是一个多方面的动态调节过程，受多种微环境因素如pH、酶和活性氧（ROS）的调节。传统的伤口敷料常常不能提供有效处理复杂伤口所需的精确干预措施。壳聚糖具有较高的生物相容性、生物降解性和结构适应性，具有较强的自愈光活性，是开发智能、反应性伤口敷料的理想候选材料。本文综述了壳聚糖智能敷料的设计策略、分子机制和治疗应用。它强调了它们在感染控制、炎症减少、血管生成和组织再生中的作用。此外，我们强调了结合自适应反馈机制（pH/ROS组合，pH/酶和ROS/光热耦合）的多模态系统的重要性，并探索了它们在治疗糖尿病足溃疡，压疮和其他复杂皮肤损伤方面的临床潜力。这些发现为下一代生物反应材料的开发提供了详细的框架，促进了材料科学与个性化伤口护理策略的整合。

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引用次数: 0

Effect of nanoemulsion-based sage seed gum coating containing cinnamon essential oil on shelf life extension of strawberry 肉桂精油纳米乳基鼠尾草籽胶包衣对草莓保质期的影响

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-19 DOI: 10.1016/j.fhfh.2025.100242

Parastoo Asadi Shizari , Fatemeh Davoodi , Dayana Hassani , Shahriyar Amiri , Bahram Hassani

In this study, the antimicrobial efficacy of a nanoemulsion (NE) based on sage seed gum (WSG) containing cinnamon essential oil (CEO) was evaluated as an edible coating to extend the shelf life of strawberries. CEO at a concentration of 400 ppm was used for NE preparation. The particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency of the NE were reported to be 148.5 nm, 0.27, –27.12 mV, and 86 %, respectively. Strawberries were coated with distilled water (control), CEO (400 ppm), WSG coating, and CEO-loaded NE coating, then stored at room temperature for 9 days. Evaluations were conducted on days 0, 3, 6, and 9 to assess mass loss, total soluble solids, total mesophilic bacteria count, total mold and yeast count, pH, titratable acidity, vitamin C content, firmness, and color parameters. The results showed that over time, mass loss, total soluble solids, total mesophilic bacteria, and total mold and yeast counts increased significantly (p < 0.05), with the highest values observed in the control sample and the lowest in the NE -coated samples. Conversely, pH, titratable acidity, vitamin C content, and firmness decreased significantly (p < 0.05) over time. The lowest total phenolic content was observed in the control group, while the highest was recorded in the NE -coated strawberries at the end of the storage period. Overall, the NE coating showed the most significant preservative effect among all treatments, followed by the conventional WSG coating. These findings highlight the potential of CEO NE as an effective edible coating to extend the shelf life of strawberries.

本研究以肉桂精油（CEO）为基料，以鼠尾草籽胶（WSG）为基料制备纳米乳，对延长草莓保质期的抗菌效果进行了研究。采用浓度为400ppm的CEO制备NE。其粒径为148.5 nm，多分散指数（PDI）为0.27，zeta电位为-27.12 mV，包封效率为86%。草莓涂有蒸馏水（对照）、CEO （400 ppm）、WSG涂层和负载CEO的NE涂层，室温保存9天。在第0、3、6和9天进行评估，评估质量损失、可溶性固形物总量、嗜中温细菌总数、霉菌和酵母总数、pH值、可滴定酸度、维生素C含量、硬度和颜色参数。结果表明，随着时间的推移，质量损失、可溶性固形物总量、中温细菌总量、霉菌和酵母菌总数均显著增加（p < 0.05），其中以对照样品最高，以NE包覆样品最低。相反，随着时间的推移，pH值、可滴定酸度、维生素C含量和硬度显著降低（p < 0.05）。总酚含量以对照组最低，NE包衣草莓贮藏末期最高。总的来说，NE涂层在所有处理中具有最显著的防腐效果，其次是常规WSG涂层。这些发现强调了CEO NE作为一种有效的可食用涂层的潜力，可以延长草莓的保质期。

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引用次数: 0

Application of yeast cell-wall derived from a potential probiotic yeast isolate as a controlled release agent in a triple-layer microcapsule with potent anti S. aureus activity 从潜在的益生菌酵母分离物中提取的酵母细胞壁作为控释剂在具有有效抗金黄色葡萄球菌活性的三层微胶囊中的应用

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-09 DOI: 10.1016/j.fhfh.2025.100241

Sima Shams Shargh , Alireza Sadeghi , Mahmoud Shams Shargh , Fahimeh Hajinia , Ali Moayedi

Application of postbiotics derived from characterized probiotics (Probio) as efficient controlled release agents of the entrapped bioactives is a promising progress in microencapsulation strategies. In the present study, phylogenetic evolutionary analysis led to the identification of Pichia kudriavzevii as the potential Probio yeast isolated from fermented sprouted soybeans with 82.70 % survivability under simulated gastrointestinal (SGI) conditions and potent in vitro inhibitory activity against Staphylococcus aureus (88.21 %), which was significantly (p < 0.05) higher than those of the other foodborne bacteria tested. The yeast isolate also showed antifungal, antioxidant and suitable adhesion capabilities. Moreover, anti-ochratoxigenic activity of viable and heat-killed cells of the isolate was approved according to the HPLC-based analysis. In vivo safety of the isolate was also confirmed in accordance with the blood biochemistry parameters and liver enzymes activity of the fed-chicken compared to the control group. In vivo inhibitory activity of viable and heat-killed cells of the isolate against S. aureus was equal to 74.39 % and 89.71 %, respectively. Cross-linking of the yeast cell-wall derived from the isolate with the alginate-chitosan construct was also approved through FTIR and SEM analyses. In addition, antibacterial profile of the construct under SGI conditions confirmed targeted delivery and controlled release of the entrapped chitosan.

从益生菌中提取的生物后制剂（Probio）作为有效的生物活性控释剂是微胶囊化策略的一个有前途的进展。本研究通过系统进化分析发现，从豆芽发酵中分离出的毕赤毕赤酵母（Pichia kudriavzevii）在模拟胃肠道（SGI）条件下的存活率为82.70%，对金黄色葡萄球菌的体外抑制活性为88.21%，显著高于其他食源性细菌（p < 0.05）。酵母分离物还表现出抗真菌、抗氧化和良好的粘附能力。此外，根据hplc分析，该分离物的活细胞和热杀细胞具有抗氧化活性。与对照组相比，根据血液生化指标和肝酶活性，验证了分离物的体内安全性。该分离物对金黄色葡萄球菌的活细胞和热死细胞的体内抑制活性分别为74.39%和89.71%。通过红外光谱（FTIR）和扫描电镜（SEM）分析，证实了海藻酸-壳聚糖结构与酵母细胞壁的交联。此外，构建物在SGI条件下的抗菌特性证实了壳聚糖的靶向递送和控制释放。

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引用次数: 0

Preserving and hydrogel-matrixing the bioactive properties of aromatic medicinal halophytic herbs from the coastline of the Iberian Peninsula 来自伊比利亚半岛海岸线的芳香盐生草本植物的生物活性特性的保存和水凝胶基质

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-05 DOI: 10.1016/j.fhfh.2025.100239

Tiago Parracho , Pedro F. Cruz , Claúdia C. Peralta , Cândida G. Silva , Maria Jorge Campos , Marta Neves , Rachel Cordeiro , Daniela Trindade , Carla Moura , Zaida L. Almeida , Cidália D. Pereira , Carla Guimarães , Rui M.M. Brito , Mauro Guerra , Fernando Reboredo , Paula Veríssimo , Vânia Ribeiro , Daniela C. Vaz

Edible wild plants are part of the ethnobotanic heritage of a certain geographical area and are important sources of essential oils, antioxidants, minerals, and special flavours. Corema album (Portuguese crowberry), Crithmum maritimum (sea fennel), Eryngium maritimum (sea holly), Helichrysum italicum (curry plant) and Otanthus maritimus (cottonweed) wildly flourish along the sandy dunes of the coast of the Iberian Peninsula. These plants are locally known for their beneficial properties, with important value for food, cosmetics and/or medicinal applications. Hence, leaves of these endemic species were collected at four different locations and submitted to different preserving treatments (oven-drying, freezing, and freeze-drying). Acetonic extracts of the different plants submitted to the different post-harvesting treatments were analysed regarding their antioxidant capacities and phenolic contents. Plant extracts were also analysed by diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY-NMR). In general, freeze-drying was the best method of preserving plant minerals, antioxidants (∼4 mgVCEAC/g fw) and polyphenols (∼5 mgGAE/g fw). Minerals were quantified via energy-dispersive X-ray fluorescence spectrometry, and despite their location, all plants were rich in Ca, Cl, K, S and P. Hierarchical clustering and principal component analyses (PCA) pointed towards chemical/metabolic proximity between taxonomic families. Alginate hydrogels loaded with 0.1 % and 0.2 % (w/v) of extracts presented homogenous surface properties by scanning electron microscopy, good mechanical tensile strength (∼30 MPa) and antibacterial activity against S. aureus. Edible alginate hydrogels enriched with plant extracts hold great nutraceutical potential to be used as natural preservatives for food coating and packaging or as sources of bioactive compounds for biomedical applications.

食用野生植物是特定地理区域的民族植物遗产的一部分，是精油、抗氧化剂、矿物质和特殊风味的重要来源。Corema album（葡萄牙蔓越莓）、Crithmum maritimum（海茴香）、Eryngium maritimum（海冬青）、Helichrysum italicum（咖喱植物）和Otanthus maritimus（棉草）在伊比利亚半岛海岸的沙丘上疯狂生长。这些植物在当地以其有益的特性而闻名，具有重要的食品、化妆品和/或药用价值。因此，在四个不同的地点收集了这些特有物种的叶子，并进行了不同的保存处理（烘箱干燥，冷冻和冷冻干燥）。对不同植物采后处理的丙酮提取物的抗氧化能力和酚类物质含量进行了分析。采用扩散有序核磁共振谱（DOSY-NMR）对植物提取物进行分析。总的来说，冷冻干燥是保存植物矿物质、抗氧化剂（~ 4 mgVCEAC/g fw）和多酚（~ 5 mgGAE/g fw）的最佳方法。通过能量色散x射线荧光光谱法对矿物质进行了定量分析，结果表明，尽管这些植物位于不同的位置，但它们都富含Ca、Cl、K、S和p。层次聚类和主成分分析（PCA）表明，这些植物在化学/代谢方面具有相似性。加载0.1%和0.2% （w/v）提取物的海藻酸盐水凝胶在扫描电镜下表现出均匀的表面性能，良好的机械拉伸强度（~ 30 MPa）和对金黄色葡萄球菌的抗菌活性。富含植物提取物的食用海藻酸盐水凝胶具有巨大的营养潜力，可作为食品涂层和包装的天然防腐剂或作为生物医学应用的生物活性化合物的来源。

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引用次数: 0

Recent advances in plant protein microgels encapsulated with bioactive compounds 包封生物活性化合物的植物蛋白微凝胶研究进展

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-03 DOI: 10.1016/j.fhfh.2025.100238

Srutee Rout , Sena Bakir , Sonali Khanal , Gurjeet Kaur , Zakir Showkat Khan , Thameed Aijaz , Jyoti Sharma , Mohmad Sayeed Bhat , Farhana Mehraj , Ghazaleh Sadeghi Vahid , Dinesh Kumar , Prem Prakash Srivastav

Protein microgels and other novel food structuring methods have garnered attention to increase plant protein functionality. Microgels, small particles (0.5–5 μm) that trap a lot of solvent, are made of a three-dimensional network of cross-linked biopolymer molecules. Protein microgels exhibit structural adaptability that makes them excellent carriers for lipophilic bioactives, protecting them from degradation and instability in aqueous systems. Additionally, their tunable design allows controlled release, enhancing bioavailability and functional efficacy in food and nutraceutical applications. The integration of scientific advances with food technology positions plant protein-based microgels as transformative tools in developing sustainable, protein-rich, and functional plant-based food systems. These advances in protein microgels prompt this review of growing plant protein sources, microgel formation, stabilization technologies, encapsulation with bioactive compounds, bioactive component transport, focusing on their functional properties, structural features, and applications. Knowing these trends can help us understand how sustainable, functioning food systems will emerge and solve global environmental and nutritional challenges.

蛋白质微凝胶和其他新的食物结构方法已经引起了人们的关注，以增加植物蛋白的功能。微凝胶是一种小颗粒（0.5-5 μm），可捕获大量溶剂，由交联生物聚合物分子组成的三维网络构成。蛋白质微凝胶具有结构适应性，这使它们成为亲脂性生物活性的优良载体，保护它们免受降解和不稳定性。此外，其可调设计允许控制释放，提高食品和营养保健应用的生物利用度和功能功效。科学进步与食品技术的结合使植物蛋白微凝胶成为开发可持续、富含蛋白质和功能性植物性食品系统的变革性工具。这些蛋白质微凝胶的进展促使本文对生长中的植物蛋白来源、微凝胶的形成、稳定技术、生物活性化合物的包封、生物活性成分的运输、它们的功能特性、结构特征和应用进行综述。了解这些趋势可以帮助我们了解可持续运作的粮食系统将如何出现，并解决全球环境和营养挑战。

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引用次数: 0

Green synthesis of biocompatible silver nanoparticles from Persicaria hydropiper leaves: Therapeutic and catalytic applications 绿色合成生物相容性银纳米粒子从水仙桃叶：治疗和催化应用

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-03 DOI: 10.1016/j.fhfh.2025.100237

Shajahan Talukder , Md. Shahin Ali , Sangita Kundu , Md. Abdul Aziz , Md. Rezaul Karim , Md. Habibur Rahman , M. Ahasanur Rabbi , Md. Rowshanul Habib

The study presents a sustainable method for synthesizing biocompatible silver nanoparticles from Persicaria hydropiper leaves (PH-AgNPs) with promising anti-inflammatory, antioxidant, anticancer, antimicrobial, and catalytic potentials. UV–vis analysis confirmed PH-AgNPs formation with a surface plasmon resonance (SPR) peak at 455 nm. FTIR spectra revealed the constituents of leaf extract involved in silver ion reduction. Characterization of PH-AgNPs was done using X-ray diffraction, dynamic light scattering, scanning electron microscopy, and energy dispersive X-ray techniques. This characterization confirmed that PH-AgNPs exhibited a crystalline, spherical shape with a strong silver peak. PH-AgNPs showed blood compatibility and a non-toxic nature as compared with Triton-X. PH-AgNPs exhibited moderate anti-inflammatory and antioxidant activities compared to diclofenac sodium and ascorbic acid. PH-AgNPs also showed potential as catalysts for the degradation of harmful dyes. In the anticancer experiment, PH-AgNPs treatments significantly (p < 0.05) decreased viable EAC (Ehrlich Ascites Carcinoma) cells and lowered weight gain in mice. PH-AgNPs (p < 0.05) restored blood counts and improved liver structure in treated mice versus untreated controls. Proapoptotic genes encoding p53 and Bax proteins were overexpressed in treated EAC cells, confirming the apoptotic hallmarks seen in fluorescence microscope images of these treated cells. PH-AgNPs demonstrated potent antibacterial activity, particularly against Shigella boydii, while erythromycin was the most effective against the same bacterium. PH-AgNPs combined with tetracycline showed the highest synergistic effect (37.26 %) against Escherichia coli. PH-AgNPs showed the lowest minimum inhibitory concentration (9.38 mg/mL) against Bacillus cereus. Therefore, the green-fabricated PH-AgNPs hold significant biological and environmental importance, offering potential for therapeutic and environmentally friendly applications.

该研究提出了一种可持续的方法来合成具有抗炎、抗氧化、抗癌、抗菌和催化潜力的生物相容性银纳米颗粒。紫外-可见分析证实了PH-AgNPs的形成，表面等离子体共振（SPR）峰在455nm处。FTIR光谱揭示了叶提取物参与银离子还原的成分。通过x射线衍射、动态光散射、扫描电子显微镜和能量色散x射线技术对PH-AgNPs进行了表征。这一表征证实了PH-AgNPs呈现出具有强银峰的结晶球形。与Triton-X相比，PH-AgNPs具有血液相容性和无毒性。与双氯芬酸钠和抗坏血酸相比，PH-AgNPs表现出中等的抗炎和抗氧化活性。PH-AgNPs还显示出作为降解有害染料催化剂的潜力。在抗癌实验中，PH-AgNPs处理显著（p < 0.05）降低了小鼠的EAC（埃利希腹水癌）活细胞，降低了小鼠的体重增加。PH-AgNPs （p < 0.05）恢复了治疗小鼠的血细胞计数，改善了肝脏结构。编码p53和Bax蛋白的促凋亡基因在处理过的EAC细胞中过度表达，证实了这些处理过的细胞的荧光显微镜图像中所见的凋亡特征。PH-AgNPs显示出强大的抗菌活性，特别是对博伊地志贺氏菌，而红霉素对同一细菌最有效。PH-AgNPs联合四环素对大肠杆菌的增效效果最高（37.26%）。PH-AgNPs对蜡样芽孢杆菌的最低抑菌浓度为9.38 mg/mL。因此，绿色制造的PH-AgNPs具有重要的生物学和环境意义，为治疗和环境友好应用提供了潜力。

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