Food Hydrocolloids for Health最新文献_第3页

Effect of nanoemulsion-based sage seed gum coating containing cinnamon essential oil on shelf life extension of strawberry 肉桂精油纳米乳基鼠尾草籽胶包衣对草莓保质期的影响

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-19 DOI: 10.1016/j.fhfh.2025.100242

Parastoo Asadi Shizari , Fatemeh Davoodi , Dayana Hassani , Shahriyar Amiri , Bahram Hassani

In this study, the antimicrobial efficacy of a nanoemulsion (NE) based on sage seed gum (WSG) containing cinnamon essential oil (CEO) was evaluated as an edible coating to extend the shelf life of strawberries. CEO at a concentration of 400 ppm was used for NE preparation. The particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency of the NE were reported to be 148.5 nm, 0.27, –27.12 mV, and 86 %, respectively. Strawberries were coated with distilled water (control), CEO (400 ppm), WSG coating, and CEO-loaded NE coating, then stored at room temperature for 9 days. Evaluations were conducted on days 0, 3, 6, and 9 to assess mass loss, total soluble solids, total mesophilic bacteria count, total mold and yeast count, pH, titratable acidity, vitamin C content, firmness, and color parameters. The results showed that over time, mass loss, total soluble solids, total mesophilic bacteria, and total mold and yeast counts increased significantly (p < 0.05), with the highest values observed in the control sample and the lowest in the NE -coated samples. Conversely, pH, titratable acidity, vitamin C content, and firmness decreased significantly (p < 0.05) over time. The lowest total phenolic content was observed in the control group, while the highest was recorded in the NE -coated strawberries at the end of the storage period. Overall, the NE coating showed the most significant preservative effect among all treatments, followed by the conventional WSG coating. These findings highlight the potential of CEO NE as an effective edible coating to extend the shelf life of strawberries.

本研究以肉桂精油（CEO）为基料，以鼠尾草籽胶（WSG）为基料制备纳米乳，对延长草莓保质期的抗菌效果进行了研究。采用浓度为400ppm的CEO制备NE。其粒径为148.5 nm，多分散指数（PDI）为0.27，zeta电位为-27.12 mV，包封效率为86%。草莓涂有蒸馏水（对照）、CEO （400 ppm）、WSG涂层和负载CEO的NE涂层，室温保存9天。在第0、3、6和9天进行评估，评估质量损失、可溶性固形物总量、嗜中温细菌总数、霉菌和酵母总数、pH值、可滴定酸度、维生素C含量、硬度和颜色参数。结果表明，随着时间的推移，质量损失、可溶性固形物总量、中温细菌总量、霉菌和酵母菌总数均显著增加（p < 0.05），其中以对照样品最高，以NE包覆样品最低。相反，随着时间的推移，pH值、可滴定酸度、维生素C含量和硬度显著降低（p < 0.05）。总酚含量以对照组最低，NE包衣草莓贮藏末期最高。总的来说，NE涂层在所有处理中具有最显著的防腐效果，其次是常规WSG涂层。这些发现强调了CEO NE作为一种有效的可食用涂层的潜力，可以延长草莓的保质期。

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引用次数: 0

Application of yeast cell-wall derived from a potential probiotic yeast isolate as a controlled release agent in a triple-layer microcapsule with potent anti S. aureus activity 从潜在的益生菌酵母分离物中提取的酵母细胞壁作为控释剂在具有有效抗金黄色葡萄球菌活性的三层微胶囊中的应用

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-09 DOI: 10.1016/j.fhfh.2025.100241

Sima Shams Shargh , Alireza Sadeghi , Mahmoud Shams Shargh , Fahimeh Hajinia , Ali Moayedi

Application of postbiotics derived from characterized probiotics (Probio) as efficient controlled release agents of the entrapped bioactives is a promising progress in microencapsulation strategies. In the present study, phylogenetic evolutionary analysis led to the identification of Pichia kudriavzevii as the potential Probio yeast isolated from fermented sprouted soybeans with 82.70 % survivability under simulated gastrointestinal (SGI) conditions and potent in vitro inhibitory activity against Staphylococcus aureus (88.21 %), which was significantly (p < 0.05) higher than those of the other foodborne bacteria tested. The yeast isolate also showed antifungal, antioxidant and suitable adhesion capabilities. Moreover, anti-ochratoxigenic activity of viable and heat-killed cells of the isolate was approved according to the HPLC-based analysis. In vivo safety of the isolate was also confirmed in accordance with the blood biochemistry parameters and liver enzymes activity of the fed-chicken compared to the control group. In vivo inhibitory activity of viable and heat-killed cells of the isolate against S. aureus was equal to 74.39 % and 89.71 %, respectively. Cross-linking of the yeast cell-wall derived from the isolate with the alginate-chitosan construct was also approved through FTIR and SEM analyses. In addition, antibacterial profile of the construct under SGI conditions confirmed targeted delivery and controlled release of the entrapped chitosan.

从益生菌中提取的生物后制剂（Probio）作为有效的生物活性控释剂是微胶囊化策略的一个有前途的进展。本研究通过系统进化分析发现，从豆芽发酵中分离出的毕赤毕赤酵母（Pichia kudriavzevii）在模拟胃肠道（SGI）条件下的存活率为82.70%，对金黄色葡萄球菌的体外抑制活性为88.21%，显著高于其他食源性细菌（p < 0.05）。酵母分离物还表现出抗真菌、抗氧化和良好的粘附能力。此外，根据hplc分析，该分离物的活细胞和热杀细胞具有抗氧化活性。与对照组相比，根据血液生化指标和肝酶活性，验证了分离物的体内安全性。该分离物对金黄色葡萄球菌的活细胞和热死细胞的体内抑制活性分别为74.39%和89.71%。通过红外光谱（FTIR）和扫描电镜（SEM）分析，证实了海藻酸-壳聚糖结构与酵母细胞壁的交联。此外，构建物在SGI条件下的抗菌特性证实了壳聚糖的靶向递送和控制释放。

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引用次数: 0

Preserving and hydrogel-matrixing the bioactive properties of aromatic medicinal halophytic herbs from the coastline of the Iberian Peninsula 来自伊比利亚半岛海岸线的芳香盐生草本植物的生物活性特性的保存和水凝胶基质

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-05 DOI: 10.1016/j.fhfh.2025.100239

Tiago Parracho , Pedro F. Cruz , Claúdia C. Peralta , Cândida G. Silva , Maria Jorge Campos , Marta Neves , Rachel Cordeiro , Daniela Trindade , Carla Moura , Zaida L. Almeida , Cidália D. Pereira , Carla Guimarães , Rui M.M. Brito , Mauro Guerra , Fernando Reboredo , Paula Veríssimo , Vânia Ribeiro , Daniela C. Vaz

Edible wild plants are part of the ethnobotanic heritage of a certain geographical area and are important sources of essential oils, antioxidants, minerals, and special flavours. Corema album (Portuguese crowberry), Crithmum maritimum (sea fennel), Eryngium maritimum (sea holly), Helichrysum italicum (curry plant) and Otanthus maritimus (cottonweed) wildly flourish along the sandy dunes of the coast of the Iberian Peninsula. These plants are locally known for their beneficial properties, with important value for food, cosmetics and/or medicinal applications. Hence, leaves of these endemic species were collected at four different locations and submitted to different preserving treatments (oven-drying, freezing, and freeze-drying). Acetonic extracts of the different plants submitted to the different post-harvesting treatments were analysed regarding their antioxidant capacities and phenolic contents. Plant extracts were also analysed by diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY-NMR). In general, freeze-drying was the best method of preserving plant minerals, antioxidants (∼4 mgVCEAC/g fw) and polyphenols (∼5 mgGAE/g fw). Minerals were quantified via energy-dispersive X-ray fluorescence spectrometry, and despite their location, all plants were rich in Ca, Cl, K, S and P. Hierarchical clustering and principal component analyses (PCA) pointed towards chemical/metabolic proximity between taxonomic families. Alginate hydrogels loaded with 0.1 % and 0.2 % (w/v) of extracts presented homogenous surface properties by scanning electron microscopy, good mechanical tensile strength (∼30 MPa) and antibacterial activity against S. aureus. Edible alginate hydrogels enriched with plant extracts hold great nutraceutical potential to be used as natural preservatives for food coating and packaging or as sources of bioactive compounds for biomedical applications.

食用野生植物是特定地理区域的民族植物遗产的一部分，是精油、抗氧化剂、矿物质和特殊风味的重要来源。Corema album（葡萄牙蔓越莓）、Crithmum maritimum（海茴香）、Eryngium maritimum（海冬青）、Helichrysum italicum（咖喱植物）和Otanthus maritimus（棉草）在伊比利亚半岛海岸的沙丘上疯狂生长。这些植物在当地以其有益的特性而闻名，具有重要的食品、化妆品和/或药用价值。因此，在四个不同的地点收集了这些特有物种的叶子，并进行了不同的保存处理（烘箱干燥，冷冻和冷冻干燥）。对不同植物采后处理的丙酮提取物的抗氧化能力和酚类物质含量进行了分析。采用扩散有序核磁共振谱（DOSY-NMR）对植物提取物进行分析。总的来说，冷冻干燥是保存植物矿物质、抗氧化剂（~ 4 mgVCEAC/g fw）和多酚（~ 5 mgGAE/g fw）的最佳方法。通过能量色散x射线荧光光谱法对矿物质进行了定量分析，结果表明，尽管这些植物位于不同的位置，但它们都富含Ca、Cl、K、S和p。层次聚类和主成分分析（PCA）表明，这些植物在化学/代谢方面具有相似性。加载0.1%和0.2% （w/v）提取物的海藻酸盐水凝胶在扫描电镜下表现出均匀的表面性能，良好的机械拉伸强度（~ 30 MPa）和对金黄色葡萄球菌的抗菌活性。富含植物提取物的食用海藻酸盐水凝胶具有巨大的营养潜力，可作为食品涂层和包装的天然防腐剂或作为生物医学应用的生物活性化合物的来源。

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引用次数: 0

Recent advances in plant protein microgels encapsulated with bioactive compounds 包封生物活性化合物的植物蛋白微凝胶研究进展

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-03 DOI: 10.1016/j.fhfh.2025.100238

Srutee Rout , Sena Bakir , Sonali Khanal , Gurjeet Kaur , Zakir Showkat Khan , Thameed Aijaz , Jyoti Sharma , Mohmad Sayeed Bhat , Farhana Mehraj , Ghazaleh Sadeghi Vahid , Dinesh Kumar , Prem Prakash Srivastav

Protein microgels and other novel food structuring methods have garnered attention to increase plant protein functionality. Microgels, small particles (0.5–5 μm) that trap a lot of solvent, are made of a three-dimensional network of cross-linked biopolymer molecules. Protein microgels exhibit structural adaptability that makes them excellent carriers for lipophilic bioactives, protecting them from degradation and instability in aqueous systems. Additionally, their tunable design allows controlled release, enhancing bioavailability and functional efficacy in food and nutraceutical applications. The integration of scientific advances with food technology positions plant protein-based microgels as transformative tools in developing sustainable, protein-rich, and functional plant-based food systems. These advances in protein microgels prompt this review of growing plant protein sources, microgel formation, stabilization technologies, encapsulation with bioactive compounds, bioactive component transport, focusing on their functional properties, structural features, and applications. Knowing these trends can help us understand how sustainable, functioning food systems will emerge and solve global environmental and nutritional challenges.

蛋白质微凝胶和其他新的食物结构方法已经引起了人们的关注，以增加植物蛋白的功能。微凝胶是一种小颗粒（0.5-5 μm），可捕获大量溶剂，由交联生物聚合物分子组成的三维网络构成。蛋白质微凝胶具有结构适应性，这使它们成为亲脂性生物活性的优良载体，保护它们免受降解和不稳定性。此外，其可调设计允许控制释放，提高食品和营养保健应用的生物利用度和功能功效。科学进步与食品技术的结合使植物蛋白微凝胶成为开发可持续、富含蛋白质和功能性植物性食品系统的变革性工具。这些蛋白质微凝胶的进展促使本文对生长中的植物蛋白来源、微凝胶的形成、稳定技术、生物活性化合物的包封、生物活性成分的运输、它们的功能特性、结构特征和应用进行综述。了解这些趋势可以帮助我们了解可持续运作的粮食系统将如何出现，并解决全球环境和营养挑战。

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引用次数: 0

Green synthesis of biocompatible silver nanoparticles from Persicaria hydropiper leaves: Therapeutic and catalytic applications 绿色合成生物相容性银纳米粒子从水仙桃叶：治疗和催化应用

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-09-03 DOI: 10.1016/j.fhfh.2025.100237

Shajahan Talukder , Md. Shahin Ali , Sangita Kundu , Md. Abdul Aziz , Md. Rezaul Karim , Md. Habibur Rahman , M. Ahasanur Rabbi , Md. Rowshanul Habib

The study presents a sustainable method for synthesizing biocompatible silver nanoparticles from Persicaria hydropiper leaves (PH-AgNPs) with promising anti-inflammatory, antioxidant, anticancer, antimicrobial, and catalytic potentials. UV–vis analysis confirmed PH-AgNPs formation with a surface plasmon resonance (SPR) peak at 455 nm. FTIR spectra revealed the constituents of leaf extract involved in silver ion reduction. Characterization of PH-AgNPs was done using X-ray diffraction, dynamic light scattering, scanning electron microscopy, and energy dispersive X-ray techniques. This characterization confirmed that PH-AgNPs exhibited a crystalline, spherical shape with a strong silver peak. PH-AgNPs showed blood compatibility and a non-toxic nature as compared with Triton-X. PH-AgNPs exhibited moderate anti-inflammatory and antioxidant activities compared to diclofenac sodium and ascorbic acid. PH-AgNPs also showed potential as catalysts for the degradation of harmful dyes. In the anticancer experiment, PH-AgNPs treatments significantly (p < 0.05) decreased viable EAC (Ehrlich Ascites Carcinoma) cells and lowered weight gain in mice. PH-AgNPs (p < 0.05) restored blood counts and improved liver structure in treated mice versus untreated controls. Proapoptotic genes encoding p53 and Bax proteins were overexpressed in treated EAC cells, confirming the apoptotic hallmarks seen in fluorescence microscope images of these treated cells. PH-AgNPs demonstrated potent antibacterial activity, particularly against Shigella boydii, while erythromycin was the most effective against the same bacterium. PH-AgNPs combined with tetracycline showed the highest synergistic effect (37.26 %) against Escherichia coli. PH-AgNPs showed the lowest minimum inhibitory concentration (9.38 mg/mL) against Bacillus cereus. Therefore, the green-fabricated PH-AgNPs hold significant biological and environmental importance, offering potential for therapeutic and environmentally friendly applications.

该研究提出了一种可持续的方法来合成具有抗炎、抗氧化、抗癌、抗菌和催化潜力的生物相容性银纳米颗粒。紫外-可见分析证实了PH-AgNPs的形成，表面等离子体共振（SPR）峰在455nm处。FTIR光谱揭示了叶提取物参与银离子还原的成分。通过x射线衍射、动态光散射、扫描电子显微镜和能量色散x射线技术对PH-AgNPs进行了表征。这一表征证实了PH-AgNPs呈现出具有强银峰的结晶球形。与Triton-X相比，PH-AgNPs具有血液相容性和无毒性。与双氯芬酸钠和抗坏血酸相比，PH-AgNPs表现出中等的抗炎和抗氧化活性。PH-AgNPs还显示出作为降解有害染料催化剂的潜力。在抗癌实验中，PH-AgNPs处理显著（p < 0.05）降低了小鼠的EAC（埃利希腹水癌）活细胞，降低了小鼠的体重增加。PH-AgNPs （p < 0.05）恢复了治疗小鼠的血细胞计数，改善了肝脏结构。编码p53和Bax蛋白的促凋亡基因在处理过的EAC细胞中过度表达，证实了这些处理过的细胞的荧光显微镜图像中所见的凋亡特征。PH-AgNPs显示出强大的抗菌活性，特别是对博伊地志贺氏菌，而红霉素对同一细菌最有效。PH-AgNPs联合四环素对大肠杆菌的增效效果最高（37.26%）。PH-AgNPs对蜡样芽孢杆菌的最低抑菌浓度为9.38 mg/mL。因此，绿色制造的PH-AgNPs具有重要的生物学和环境意义，为治疗和环境友好应用提供了潜力。

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引用次数: 0

Structural characterization and bioactivity of sulfated Galactan GSSG-2 from Gracilaria salicornia: Antioxidant and anticancer potential 盐角江蓠硫酸半乳糖GSSG-2的结构表征和生物活性：抗氧化和抗癌潜力

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-08-08 DOI: 10.1016/j.fhfh.2025.100236

Mythileeswari Lakshmikanthan , Sakthivel Muthu , Peer Mohamed Deen Mohamed , Kathiravan Krishnan , Nagaraj Karuppiah , Ramamoorthy Karuppan , Shenbhagaraman Ramalingam , Anandhi Subramanian , Bharathi Venkatachalam , Gholamreza Abdi

The sulfated galactan GSSG-2, isolated from Gracilaria salicornia, was investigated for its antioxidant and anticancer properties. Polysaccharides derived from marine sources are well-known for their distinctive bioactivities, making them promising candidates for pharmaceutical applications. Purification of GSSG-2 was achieved through a two-step procedure consisting of anion-exchange chromatography utilizing a DEAE-Sepharose column, followed by size-based separation via Sephadex G-100 gel filtration chromatography. Its structural features were characterized using elemental analysis, high-performance gel permeation chromatography (HPGPC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) spectroscopy. The antioxidant capacity of GSSG-2 was measured at concentrations from 25 to 150 µg/mL using assays such as DPPH radical scavenging, inhibition of superoxide anions, ferric reducing antioxidant power (FRAP), and total reducing power. In all assays, l-ascorbic acid served as the reference standard. Cytotoxic effects and induction of apoptosis in A549 human lung carcinoma cells were assessed employing MTT assays and acridine orange/ethidium bromide (AO/EtBr) fluorescence staining. GSSG-2 demonstrated a molecular weight of 70.61 kDa, with galactose identified as the dominant monosaccharide unit (328.92 mg/g). Its antioxidant activity showed a dose-dependent increase, ranging from 19.84% to 88.59%. The IC₅₀ value determined by the MTT assay was 100 µg/mL, and cell viability dropped dramatically to 16.0% at 250 µg/mL. Results from AO/EtBr staining further corroborated the time-dependent enhancement of apoptotic cell death. The strong antioxidant and anticancer effects of GSSG-2 are attributed to its structural components, notably the esterified sulfate moieties and β-(1→3)-linked d-galactopyranose units. These results underscore the potential of this compound as a therapeutic agent targeting oxidative stress and cancer.

研究了从荆芥中分离得到的硫酸酸化半乳多糖GSSG-2的抗氧化和抗癌性能。从海洋中提取的多糖以其独特的生物活性而闻名，这使它们成为制药应用的有希望的候选者。GSSG-2的纯化是通过两步程序完成的，包括使用DEAE-Sepharose柱的阴离子交换层析，然后使用Sephadex G-100凝胶过滤层析进行基于尺寸的分离。采用元素分析、高效凝胶渗透色谱（HPGPC）、高效液相色谱（HPLC）、傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）等方法对其结构特征进行了表征。在25 ~ 150µg/mL浓度范围内测定GSSG-2的抗氧化能力，包括清除DPPH自由基、抑制超氧阴离子、铁还原抗氧化能力（FRAP）和总还原能力。在所有的测定中，l-抗坏血酸作为参比标准。采用MTT法和吖啶橙/溴化乙啶（AO/EtBr）荧光染色法研究了A549人肺癌细胞的细胞毒作用和诱导凋亡的作用。GSSG-2的分子量为70.61 kDa，其中半乳糖为优势单糖单位（328.92 mg/g）。其抗氧化活性呈剂量依赖性，增加幅度在19.84% ~ 88.59%之间。MTT测定的IC₅0值为100µg/mL，细胞活力在250µg/mL时急剧下降至16.0%。AO/EtBr染色结果进一步证实了凋亡细胞死亡的时间依赖性增强。GSSG-2具有较强的抗氧化和抗癌作用，主要归功于其结构成分，特别是酯化硫酸盐部分和β-(1→3)-连接的d-半乳糖糖单元。这些结果强调了该化合物作为靶向氧化应激和癌症的治疗剂的潜力。

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引用次数: 0

Extraction, purification, structure characterization of polysaccharides from Cistanche deserticola and their biological effects 肉苁蓉多糖的提取、纯化、结构表征及其生物学效应

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-07-30 DOI: 10.1016/j.fhfh.2025.100235

Faqin Tao , Baotang Zhao , Shen Song , Yunfei Xu , Ji Zhang

The polysaccharide fraction (CDP-D2-N1) from the stem of Cristanche deseticola was obtained after extraction and purification. The structural characteristics and in vitro antioxidant and anti-inflammatory activities of CDP-D2-N1 were investigated by physical, chemical and instrumental analyses. The results showed that CDP-D2-N1 (127.784 kDa) is a heterogeneous polysaccharide with an irregular non-crystalline structure, containing mainly Rha:Ara:Xyl:Gal:Gal:GlcA:Glc:GalA, and a molar ratio of 6.75 : 26.68 : 1.56 : 37.87 : 3.94 : 2.17 : 21.02. Combined with the results of NMR spectroscopy and methylation analyses, the main chain of CDP-D2-N1 was determined to be →4)-α-d-GalpA-(1→,→3,6)-β-d-Galp-(1→, →5)-α-l-Araf-(1→, containing a small amount of fragment →3,4)-β-d-Galp-(1→ and →3)-β-d-Galp-(1→, and the CDP-D2-N1 structure was inferred. In vitro assays demonstrated that CDP-D2-N1 possessed significant antioxidant capacity and attenuated inflammation induced by lipopolysaccharide (LPS) from Escherichia coli O55:B5 in bovine endothelial cells (bEEcs) by inhibiting the expression levels of TNF-α, IL-6, TLR 4 and IL-1.

提取纯化后，得到了地衣牡丹茎多糖部分（CDP-D2-N1）。采用物理、化学和仪器分析方法研究了CDP-D2-N1的结构特征及体外抗氧化和抗炎活性。结果表明，CDP-D2-N1 （127.784 kDa）是一种不规则的非晶体结构的多相多糖，主要成分为Rha:Ara:Xyl:Gal:Gal:GlcA:Glc:GalA，摩尔比为6.75:26.68:1.56:37.87:3.94:2.17:21.02。结合核磁共振波谱和甲基化分析结果，确定了CDP-D2-N1的主链为→4)-α-d- galpa -(1→，→3,6)-β-d- galp -(1→，→5)-α-l- araf -（1→），含有少量片段→3,4)-β-d- galp -(1→和→3)-β-d- galp -（1→），推断了CDP-D2-N1的结构。体外实验表明，CDP-D2-N1通过抑制TNF-α、IL-6、tlr4和IL-1的表达，具有显著的抗氧化能力，减轻了大肠杆菌O55:B5脂多糖（LPS）在牛内皮细胞（bEEcs）中的炎症反应。

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引用次数: 0

Proteins derived from green biomass: Alfalfa (Medicago sativa L.) and water lentil concentrate (Lemna minor L.) in the focus as stabilizers for emulsions 从绿色生物质中提取的蛋白质：苜蓿（Medicago sativa L.）和水扁豆浓缩物（Lemna minor L.）作为乳剂的稳定剂

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-07-24 DOI: 10.1016/j.fhfh.2025.100233

Maren Müller , Olaf Holderer , Kuno Schwärzer , Anika Wiese-Klinkenberg , Beate Förster , Stephan Förster , Joachim Kohlbrecher , Kathleen Wood , Baohu Wu , Stephan Hauschild , Henrich Frielinghaus , Theresia Heiden-Hecht

Green crop biomass is a sustainable protein source, which can support solving global food challenges as well as improving the bioeconomy for novel food utilization. Two green biomasses -alfalfa and water lentil- known for a high protein content and their value as feed and food components were investigated for their potential in stabilizing emulsions.

For interfaces in complex food systems like emulsions, the proteins from the green biomasses act similarly to other plant proteins and even to some extent to whey proteins. The extracted green biomass protein concentrates were composed of a diverse mixture of proteins: RuBisCo as the main component, enzymes like ATP subunit synthase, chlorophyll a-b binding proteins, histones and cytochromes, which all were associated as protein aggregates or protein-polyphenol complexes with sizes from 33 up to 85 nm.

These multicomponent and complex green protein components were obtained in an extraction process, and proved their capability to stabilize emulsions. Key parameters are a narrow oil droplet size distribution with rather small oil droplets with a median of about 3.5 μm, a low interfacial tension with around 15 mN/m and an elastic interfacial layer with an elastic modulus of about 45 mN/m comparable to functional whey or plant proteins. The oil/water interface of the emulsion revealed a more pronounced structural rearrangement for alfalfa protein compared to water lentil proteins as quantified by small-angle neutron scattering (SANS), indicating the relevance of plant protein source to the final structure.

Green biomass proteins from alfalfa and water lentil represent a novel protein source to stabilize emulsions.

绿色作物生物质是一种可持续的蛋白质来源，可以支持解决全球粮食挑战，并改善生物经济以实现新的粮食利用。两种绿色生物质-紫花苜蓿和水扁豆-以蛋白质含量高而闻名，它们作为饲料和食品成分的价值被研究了它们在稳定乳剂中的潜力。对于乳剂等复杂食物系统的界面，来自绿色生物质的蛋白质的作用与其他植物蛋白质相似，甚至在某种程度上与乳清蛋白相似。提取的绿色生物质蛋白浓缩物由多种蛋白质组成：RuBisCo为主要成分，ATP亚基合成酶、叶绿素a-b结合蛋白、组蛋白和细胞色素等酶，它们都以蛋白质聚集体或蛋白质-多酚复合物的形式存在，大小从33 ~ 85 nm不等。在萃取过程中得到了这些多组分和复杂的绿色蛋白组分，并证明了它们具有稳定乳状液的能力。关键参数是油滴尺寸分布窄，油滴中值约为3.5 μm，界面张力低，约为15 mN/m，弹性界面层弹性模量约为45 mN/m，与功能乳清或植物蛋白相当。小角中子散射（SANS）结果表明，与水扁豆蛋白相比，苜蓿蛋白在乳状液的油水界面结构重排更为明显，表明植物蛋白来源与乳状液的最终结构相关。从苜蓿和扁豆中提取的绿色生物质蛋白是一种稳定乳状液的新型蛋白质来源。

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引用次数: 0

The action of Poria cocos polysaccharides against non-alcoholic fatty liver disease: A preliminary study 茯苓多糖抗非酒精性脂肪肝作用的初步研究

IF 4.6 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-07-23 DOI: 10.1016/j.fhfh.2025.100234

Rui Zhou , Qinting Chen , Jie Mo , Meizhen Liu , Ka Wu , Chao Guo

Non-alcoholic fatty liver disease (NAFLD) is a significant public health challenge owing to its current prevalence. Basic and clinical evidence indicates that pyroptosis is responsible for the development of NAFLD to non-alcoholic steatohepatitis. Our previous findings suggested that Poria cocos polysaccharides (PCP) exert promising anti-liver injury benefits. However, the anti-NAFLD action of PCP via the modulation of pyroptosis remains untested. In this study, a network pharmacology- and molecular docking-based approach was used to determine the anti-NAFLD activities and mechanisms of PCP prior to in vitro or in vivo. Based on these bioinformatics data, 31 intersecting genes in PCP (483 target genes), NAFLD (1445 target genes), and pyroptosis (905 target genes) were identified via network pharmacology analysis, and a total of 16 key genes in PCP against NAFLD through the modulation of pyroptosis were determined. Further enrichment analysis revealed the detailed anti-NAFLD functions and molecular mechanisms of PCP. Molecular docking simulations were used to identify the spatial binding features between PCP and the target proteins related to pyroptosis. This study determined the pyroptosis-related cluster genes that act with PCP against NAFLD, thereby offering preliminary evidence to support PCP’s potential in treating NAFLD. Additionally, we identified the possible underlying pyroptotic mechanisms for future experimental validations.

非酒精性脂肪性肝病（NAFLD）是一个重大的公共卫生挑战，由于其目前的流行。基础和临床证据表明，焦亡是NAFLD发展为非酒精性脂肪性肝炎的原因。我们的研究结果表明茯苓多糖（PCP）具有良好的抗肝损伤作用。然而，PCP通过调节焦亡的抗nafld作用尚未得到验证。在本研究中，采用基于网络药理学和分子对接的方法来确定PCP在体外或体内的抗nafld活性和机制。基于这些生物信息学数据，通过网络药理学分析，鉴定出PCP（483个靶基因）、NAFLD（1445个靶基因）和焦亡（905个靶基因）中31个交叉基因，确定了PCP通过调节焦亡对抗NAFLD的16个关键基因。进一步的富集分析揭示了PCP抗nafld的详细功能和分子机制。通过分子对接模拟，确定PCP与焦亡相关靶蛋白之间的空间结合特征。本研究确定了与PCP一起作用于NAFLD的焦热相关簇基因，从而为支持PCP治疗NAFLD的潜力提供了初步证据。此外，我们为未来的实验验证确定了可能的潜在热亡机制。

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引用次数: 0

Pectins mitigate Candidalysin-induced gut barrier disruption and inflammation in vitro in a degree of methyl-esterification-dependent manner 果胶在体外以一定程度的甲基酯化依赖方式减轻念珠菌素诱导的肠道屏障破坏和炎症

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health

Pub Date : 2025-07-23 DOI: 10.1016/j.fhfh.2025.100232

Naschla Gasaly , Xiaochen Chen , Luis Silva-Lagos , Isaura Beatriz Borges Silva , Marcela A. Hermoso , Paul de Vos

Pectin, a dietary fiber, exhibits anti-inflammatory properties influenced by its degree of methyl-esterification (DM). Inflammatory bowel disease (IBD) is characterized by chronic inflammation due to a compromised epithelial barrier, dysregulated microbiota, and an overactive immune response. Beyond bacterial dysbiosis, recent research emphasizes the gut mycobiota, particularly Candida albicans, in IBD progression. C. albicans is frequently elevated in IBD patients and secretes candidalysin (CaLysin), a cytolytic toxin that disrupts epithelial barrier integrity. This study evaluates the protective effects of lemon-derived pectins with low (DM18) and high (DM88) DM against CaLysin-induced epithelial-stress in vitro. T84 cell monolayers were pre-treated with pectins prior to CaLysin challenge. CaLysin impaired barrier function, increased lactate dehydrogenase (LDH) release, and pro-inflammatory cytokines (e.g., IL-8, IL-33), while downregulating tight junction proteins (Claudin-1, Occludin) and tissue repair markers such as Retinaldehyde dehydrogenase 1 (ALDH1A1) and Amphiregulin (AREG). Both pectins mitigated these effects, preserving barrier integrity, reducing LDH release, and cytokine expression (e.g., IL-8, IL-13, IL-18, CCL20). Pectins also upregulated tight junction proteins (Claudin-1, Occludin, Zonula Occludens-1) at mRNA and protein levels in a DM-dependent manner and enhanced tissue repair genes. These findings highlight the potential of pectins, particularly those with low-DM (DM18), in protecting against CaLysin-induced gut epithelial damage.

果胶是一种膳食纤维，其抗炎特性受其甲基酯化程度（DM）的影响。炎症性肠病（IBD）的特征是由上皮屏障受损、微生物群失调和过度活跃的免疫反应引起的慢性炎症。除了细菌生态失调，最近的研究强调肠道菌群，特别是白色念珠菌，在IBD的进展中。白色念珠菌在IBD患者中经常升高，并分泌念珠菌素（CaLysin），这是一种破坏上皮屏障完整性的细胞溶解毒素。本研究评估了低（DM18）和高（DM88） DM的柠檬衍生果胶对calysin诱导的体外上皮应激的保护作用。在CaLysin攻毒前，用果胶对T84细胞单层进行预处理。CaLysin损伤屏障功能，增加乳酸脱氢酶（LDH）释放和促炎细胞因子（如IL-8， IL-33），同时下调紧密连接蛋白（Claudin-1, Occludin）和组织修复标志物，如视黄醛脱氢酶1 （ALDH1A1）和双调节蛋白（AREG）。两种果胶都减轻了这些影响，保持屏障完整性，减少LDH释放和细胞因子表达（例如，IL-8, IL-13, IL-18, CCL20）。果胶还以dm依赖的方式上调紧密连接蛋白（Claudin-1、Occludin、Zonula Occludens-1）的mRNA和蛋白水平，并增强组织修复基因。这些发现强调了果胶的潜力，特别是那些低dm （DM18）的果胶，在防止calysin诱导的肠道上皮损伤方面。

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引用次数: 0