Food Science and Human Wellness最新文献_第5页

Oral administration of egg white ovotransferrin prevents osteoporosis in ovariectomized rats 口服蛋白卵铁蛋白可预防卵巢切除大鼠骨质疏松症

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250249

Nan Shang , Xiaoyu Bao , Michael Doschak , Jianping Wu

Ovotransferrin, an iron-binding glycoprotein, accounting for approximately 12 % of egg white protein, is a member of transferrin family. Our previous studies showed that ovotransferrin stimulates the proliferation and differentiation of osteoblasts, while inhibits osteoclastogenesis and resorption activity. The work aims to study the efficacy of orally administered ovotransferrin on the prevention of osteoporosis using ovariectomized (OVX) Sprague-Dawley rats. Oral administration of ovotransferrin showed no negative effect on body weight, food intake and organ weight. After 12-week treatment, feeding ovotransferrin at a dose of 1 % (1 g ovotransferrin/100 g diet) prevented OVX-induced bone loss and maintained relatively high bone mineral density and integrated bone microarchitecture. The serum concentration of biomarkers indicating bone formation was increased in ovotransferrin administration groups, while the bone resorption biomarkers were decreased. Ovotransferrin feeding also decreased the production of serum cytokine TNF-α and IL-6, which are two stimulators for osteoclast differentiation. In addition to its direct regulatory role on bone turnover, ovotransferrin supplementation might benefit osteoporosis prevention by inhibiting adipogenesis, and regulating immune response. Our results suggested the potential application of ovotransferrin as a functional food ingredient on the prevention of osteoporosis.

卵铁蛋白是一种铁结合糖蛋白，约占蛋清蛋白的 12%，属于转铁蛋白家族。我们之前的研究表明，卵铁蛋白能刺激成骨细胞的增殖和分化，同时抑制破骨细胞的生成和吸收活性。这项工作旨在利用卵巢切除（OVX）的 Sprague-Dawley 大鼠研究口服卵磷脂对预防骨质疏松症的功效。口服卵铁蛋白对体重、食物摄入量和器官重量没有负面影响。经过12周的治疗后，喂食1%剂量的卵铁蛋白（1克卵铁蛋白/100克食物）可防止OVX诱导的骨质流失，并保持相对较高的骨矿物质密度和完整的骨微结构。卵磷转铁蛋白给药组血清中表明骨形成的生物标志物浓度增加，而骨吸收的生物标志物浓度降低。卵磷转铁蛋白还能减少血清细胞因子TNF-α和IL-6的产生，而TNF-α和IL-6是破骨细胞分化的两种刺激因子。除了对骨转换的直接调节作用外，补充卵磷转铁蛋白还能通过抑制脂肪生成和调节免疫反应来预防骨质疏松症。我们的研究结果表明，卵铁蛋白作为一种功能性食品配料，在预防骨质疏松症方面具有潜在的应用价值。

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引用次数: 0

Strain-specific effect of Streptococcus thermophilus consumption on host physiology 嗜热链球菌对宿主生理机能的影响因菌株而异

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250233

Streptococcus thermophilus is one of the most prevalent species in stool samples of westernized populations due to continuous exposure to fermented dairy products. However, few studies have explored the effect on host physiology by multiple S. thermophilus strains and considered the inter-strain differences in regulating host. In the present study, we investigated how four S. thermophilus strains influenced the gut microbiota, mucin changes, and host metabolism after 28 days of intervention in conventional mice. The results indicated that the consumption of S. thermophilus affected the host with strain specificity. Among four S. thermophilus strains, DYNDL13-4 and DQHXNQ38M61, especially DQHXNQ38M61, had more effect on host physiology by modulating gut microbiota and host metabolism than LMD9 and 4M6. Ingestion of strains DYNDL13-4 and DQHXNQ38M61 resulted in more remarkable changes in amino acid metabolism and lipid metabolism than that of strains LMD9 and 4M6, which may be related to the elevation of intestinal Bifidobacterium by DYNDL13-4 and DQHXNQ38M61. The enriched Coriobacteriaceae UCG-002, Rikenellaceae RC9 gut group, and Lactobacillus only in the DQHXNQ38M61 group, had a close relationship with the prominent effect of DQHXNQ38M61 on regulating amino acid and lipid metabolism. In addition, DQHXNQ38M61 had a strong influence on degrading colonic mucin fucose by decreased α-fucosidase activity in feces, and improving mucin sulfation by upregulated Gal3ST2 expression. Comparative genomic analysis revealed that the four S. thermophilus strains belonged to different branches in the phylogenetic tree, and DYNDL13-4 and DQHXNQ38M61 had more genes involved in carbohydrate metabolism, amino acid metabolism, membrane transport, and signal transduction, which may confer the capacity of nutrient utilization and gastrointestinal adaptation of the strains and be associated with their strong regulation in host. Our study provides valuable information for understanding the regulation of host metabolism after consuming different S. thermophilus strains and could facilitate potential personalized applications of S. thermophilus based on strain varieties.

由于持续接触发酵乳制品，嗜热链球菌是西方化人群粪便样本中最常见的菌种之一。然而，很少有研究探讨多种嗜热链球菌菌株对宿主生理的影响，也很少有研究考虑不同菌株在调节宿主方面的差异。在本研究中，我们调查了四种嗜热菌菌株在干预常规小鼠 28 天后如何影响肠道微生物群、粘蛋白变化和宿主代谢。结果表明，食用嗜热菌对宿主的影响具有菌株特异性。在四个嗜热菌菌株中，DYNDL13-4 和 DQHXNQ38M61，尤其是 DQHXNQ38M61，与 LMD9 和 4M6 相比，通过调节肠道微生物群和宿主新陈代谢对宿主生理机能的影响更大。与 LMD9 和 4M6 相比，摄入 DYNDL13-4 和 DQHXNQ38M61 菌株会导致氨基酸代谢和脂质代谢发生更显著的变化，这可能与 DYNDL13-4 和 DQHXNQ38M61 提高了肠道双歧杆菌的数量有关。DQHXNQ38M61 组中富含 Coriobacteriaceae UCG-002、Rikenellaceae RC9 肠道菌群，且仅有乳酸杆菌，这与 DQHXNQ38M61 在调节氨基酸和脂质代谢方面的突出作用有密切关系。此外，DQHXNQ38M61还通过降低粪便中α-岩藻糖苷酶的活性来降解结肠粘蛋白岩藻糖，并通过上调Gal3ST2的表达来改善粘蛋白硫酸化。基因组比较分析表明，四株嗜热菌在系统发生树中分属不同的分支，其中DYNDL13-4和DQHXNQ38M61有较多的基因涉及碳水化合物代谢、氨基酸代谢、膜转运和信号转导，这可能赋予了菌株营养物质利用能力和胃肠道适应能力，并与其在宿主体内的强调控能力有关。我们的研究为了解宿主摄入不同嗜热菌菌株后的代谢调控提供了有价值的信息，可促进基于菌株品种的嗜热菌潜在个性化应用。

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引用次数: 0

Amelioration of dextran sodium sulphate-induced colitis in mice by treatment with Lactobacillus rhamnosus and Lactobacillus reuteri: intraspecific and interspecific patterns 鼠李糖乳杆菌和沙门氏菌治疗小鼠右旋糖酐硫酸钠诱发的结肠炎：种内和种间模式

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250227

Lactobacillus rhamnosus (Rh) and Lactobacillus reuteri (Re) are well-known probiotic species in inflammatory bowel disease (IBD) research. The variations between these species’ efficacy against colitis, and their model of action in this regard, are intriguing and enable treatment to be individually tailored to patients. In this study, four strains each of Rh and Re were isolated from fecal samples and their draft genomes were sequenced. The anti-colitis activities of both strains involved various aspects of intestinal immune, physical, chemical, and biological barrier function. Strikingly, the tested strains exhibited considerable interspecies and intraspecies specificity in colitis amelioration. Rh strains significantly outperformed Re strains in terms of short-chain fatty acid synthesis. Nevertheless, Re strains were more effective than Rh strains in inhibiting production of inflammatory factors; promoting production of intestinal mucus, antimicrobial peptides, and tight junction proteins; and supporting the stem cell compartment. This accounts for the anti-colitis outcomes of Re strains being superior to those of Rh strains. In addition, the effective Rh and Re strains were found to express high concentrations of specific carbohydrate metabolism- and prophage-related genes, respectively. Taken together, the results of this study could assist researchers in developing effective therapies for IBD.

鼠李糖乳杆菌（Rh）和罗伊氏乳杆菌（Re）是炎症性肠病（IBD）研究中著名的益生菌。这两种益生菌对结肠炎的疗效及其作用模式之间的差异十分有趣，可为患者提供个性化治疗。在这项研究中，从粪便样本中分离出了 Rh 和 Re 各四株菌株，并对它们的基因组草案进行了测序。这两种菌株的抗结肠炎活性涉及肠道免疫、物理、化学和生物屏障功能的各个方面。引人注目的是，受试菌株在改善结肠炎方面表现出相当大的种间和种内特异性。在短链脂肪酸合成方面，Rh 菌株明显优于 Re 菌株。然而，在抑制炎症因子的产生、促进肠粘液、抗菌肽和紧密连接蛋白的产生以及支持干细胞区系方面，Re 菌株比 Rh 菌株更有效。这就是 Re 菌株抗结肠炎效果优于 Rh 菌株的原因。此外，还发现有效的 Rh 和 Re 菌株分别表达高浓度的特定碳水化合物代谢基因和噬菌体相关基因。综上所述，这项研究的结果有助于研究人员开发治疗肠道疾病的有效疗法。

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引用次数: 0

Mitigation of polycyclic aromatic hydrocarbons (PAHs) in roasted beef patties by cold plasma treatment and products quality evaluation 通过冷等离子处理减轻烤牛肉饼中的多环芳烃（PAHs）含量及产品质量评价

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250242

The cold plasma (CP) technique was applied to alleviate the contamination of polycyclic aromatic hydrocarbon (PAH) in this investigation. Two different CP treatments methods were implemented in the production of beef patties, to investigate their inhibition and degradation capacity on PAHs. With 5 different cooking oils and fats addition, the inhibition mechanism of in-package cold plasma (ICP) pretreatment was explored from the aspect of raw patties fatty acids composition variation. The results of principal component analysis showed that the first two principal components accounted for more than 80 % of the total variation in the original data, indicating that the content of saturated fatty acids was significantly positively correlated with the formation of PAHs. ICP pretreatment inhibited the formation of PAHs by changing the composition of fatty acids, which showed that the total amount of polyunsaturated fatty acids decreased and the total amount of monounsaturated fatty acids increased. Sensory discrimination tests demonstrated there were discernable differences between 2 CP treated samples and the controls, utilization of the ICP pretreatment in meat products processing was expected to achieve satisfying eating quality. In conclusion, CP treatment degraded PAHs through stepwise ring-opening oxidation in 2 reported pathways, the toxicity of PAHs contaminated products was alleviated after CP treatment.

本研究采用冷等离子体（CP）技术来减轻多环芳烃（PAH）污染。在牛肉饼的生产过程中采用了两种不同的冷等离子处理方法，以研究其对多环芳烃的抑制和降解能力。通过添加 5 种不同的食用油脂，从牛肉饼原料脂肪酸组成变化的角度探讨了包装内冷等离子体（ICP）预处理的抑制机理。主成分分析结果表明，前两个主成分占原始数据总变化的 80% 以上，表明饱和脂肪酸的含量与多环芳烃的形成呈显著正相关。ICP 预处理通过改变脂肪酸的组成来抑制多环芳烃的形成，结果表明多不饱和脂肪酸的总量减少了，而单不饱和脂肪酸的总量增加了。感官鉴别测试表明，经氯化石蜡处理的两个样品与对照组之间存在明显差异，在肉制品加工中使用 ICP 预处理有望获得令人满意的食用品质。总之，氯化石蜡处理通过所报道的两种途径逐步开环氧化降解多环芳烃，经氯化石蜡处理后，多环芳烃污染产品的毒性得到缓解。

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引用次数: 0

Ganoboninketal C from Ganoderma boninense improves the efficacy of CDDP-based chemotherapy through inhibiting translesion DNA synthesis 灵芝中的灵芝素 C 通过抑制转子 DNA 合成提高了 CDDP 化疗的疗效

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250241

Translesion DNA synthesis (TLS) can bypass DNA lesions caused by chemotherapeutic drugs, which usually result in drug resistance. Given its key role in mutagenesis and cell survival after DNA damage, inhibition of the TLS pathway has emerged as a potential target for improving the efficacy of DNA-damaging agents such as cisplatin (CDDP), a widely used anticancer agent. Unfortunately, few suitable natural TLS inhibitors have been reported. Here, we found that a triterpenoid compound Ganoboninketal C (26-3) from Ganoderma boninense, a traditional Chinese medicine, can impair CDDP-induced TLS polymerase eta (Polη) focus formation, PCNA monoubiquitination as well as mutagenesis. Moreover, 26-3 can significantly sensitize tumor cells to CDDP killing and reduce the proportion of cancer stem cells in AGS and promote apoptosis after CDDP exposure. Interestingly, 26-3 can also sensitize tumor cells to Gefitinib therapy. Mechanistically, through RNA-seq analysis, we found that 26-3 could abrogate the CDDP-induced upregulation of Polη and PIDD (p53-induced protein with a death domain), 2 known factors promoting TLS pathway. Furthermore, we found that activating transcription factor 3 is a potential novel TLS modulator. Taken together, we have identified a natural TLS inhibitor 26-3, which can be potentially used as an adjuvant to improve clinical efficacy.

转座子 DNA 合成（TLS）可以绕过化疗药物造成的 DNA 损伤，而这种损伤通常会导致耐药性。鉴于 TLS 在 DNA 损伤后的诱变和细胞存活中的关键作用，抑制 TLS 途径已成为提高 DNA 损伤药物（如广泛使用的抗癌药物顺铂（CDDP））疗效的潜在靶点。遗憾的是，目前鲜有合适的天然 TLS 抑制剂的报道。在这里，我们发现一种来自传统中药灵芝的三萜类化合物 Ganoboninketal C（26-3）可以损害 CDDP 诱导的 TLS 聚合酶 eta（Polη）病灶形成、PCNA 单泛素化以及诱变。此外，26-3 还能在 CDDP 暴露后使肿瘤细胞对 CDDP 杀灭显著敏感，并降低 AGS 中癌症干细胞的比例，促进细胞凋亡。有趣的是，26-3 还能使肿瘤细胞对吉非替尼治疗敏感。从机理上讲，通过RNA-seq分析，我们发现26-3可以抑制CDDP诱导的Polη和PIDD（具有死亡结构域的p53诱导蛋白）的上调，而Polη和PIDD是两种已知的促进TLS通路的因子。此外，我们还发现激活转录因子 3 是一种潜在的新型 TLS 调节因子。综上所述，我们发现了一种天然的 TLS 抑制剂 26-3，它有可能被用作提高临床疗效的辅助剂。

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引用次数: 0

Comparative proteomics analysis reveals the domesticated Lepista sordida primordium differentiation regulation mechanism and the subsequent different development patterns in the pileus and stipe 比较蛋白质组学分析揭示了驯化鳞栉水母原基分化调控机制及随后绒毛和镫骨的不同发育模式

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2023.9250051

The wild Lepista sordida is a kind of precious and rare edible fungus. An excellent strain of it by artificial domestication was obtained, which was high-yield and high in iron content. In this study, high-throughput comparative proteomics was used to reveal the regulatory mechanism of its primordium differentiation in the early fruiting body formation. The mycelium before the primordium differentiation mainly expressed high levels of mitochondrial functional proteins and carbon dioxide concentration regulatory proteins. In young mushrooms, the highly expressed proteins were mainly involved in cell component generation, cell proliferation, nitrogen compound metabolism, nucleotide metabolism, glutathione metabolism, and purine metabolism. The differential regulation patterns of pileus and stipe growth to maturity were also revealed. The highly expressed proteins related to transcription, RNA splicing, the production of various organelles, DNA conformational change, nucleosome organization, protein processing, maturation and transport, and cell detoxification regulated the pileus development and maturity. The proteins related to carbohydrate and energy metabolism, large amounts of obsolete cytoplasmic parts, nutrient deprivation, and external stimuli regulated the stipe development and maturity. Multiple CAZymes regulated nutrient absorption, morphogenesis, spore production, stress response, and other life activities at different growth and development stages.

野生雷公藤是一种珍贵稀有的食用菌。通过人工驯化，获得了高产、高铁质的优良菌株。本研究利用高通量比较蛋白质组学揭示了其子实体形成早期的初生分化调控机制。子实体分化前的菌丝主要高表达线粒体功能蛋白和二氧化碳浓度调控蛋白。在幼菇中，高表达的蛋白质主要参与细胞成分生成、细胞增殖、氮化合物代谢、核苷酸代谢、谷胱甘肽代谢和嘌呤代谢。此外，还揭示了绒毛和菌柄从生长到成熟的不同调控模式。与转录、RNA剪接、各种细胞器的产生、DNA构象变化、核糖体组织、蛋白质加工、成熟和运输以及细胞解毒有关的高表达蛋白调控着绒毛的发育和成熟。与碳水化合物和能量代谢有关的蛋白质、大量过时的细胞质部分、营养匮乏和外部刺激调控着柄的发育和成熟。多种 CAZymes 调节不同生长和发育阶段的营养吸收、形态发生、孢子产生、应激反应和其他生命活动。

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引用次数: 0

Microbiomics and metabolomics insights into the microbial regulation on the formation of flavor components in the traditional fermentation process of Chinese Hongqu aged vinegar 微生物组学和代谢组学对中国红曲陈醋传统发酵过程中风味成分形成的微生物调控的启示

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250224

This study aimed to investigate microbial succession and metabolic dynamics during the traditional fermentation of Hongqu aged vinegar, and explore the core functional microbes closely related to the formation of flavor components. Microbiome analysis demonstrated that Lactobacillus, Acetobacter, Bacillus, Enterobacter, Lactococcus, Leuconostoc and Weissella were the predominant bacterial genera, while Aspergillus piperis, Aspergillus oryzae, Monascus purpureus, Candida athensensis, C. xylopsoci, Penicillium ochrosalmoneum and Simplicillium aogashimaense were the predominant fungal species. Correlation analysis revealed that Acetobacter was positively correlated with the production of tetramethylpyrazine, acetoin and acetic acid, Lactococcus showed positive correlation with the production of 2-nonanone, 2-heptanone, ethyl caprylate, ethyl caprate, 1-hexanol, 1-octanol and 1-octen-3-ol, C. xylopsoci and C. rugosa were positively associated with the production of diethyl malonate, 2,3-butanediyl diacetate, acetoin, benzaldehyde and tetramethylpyrazine. Correspondingly, non-volatile metabolites were also detected through ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry. A variety of amino acids and functional dipeptides were identified during the traditional brewing of Hongqu aged vinegar. Correlation analysis revealed that Lactobacillus was significantly associated with DL-lactate, indolelactic acid, D-(+)-3-phenyllactic acid, pimelic acid, pregabalin and 3-aminobutanoic acid. This study is useful for understanding flavor formation mechanism and developing effective strategies for the suitable strains selection to improve the flavor quality of Hongqu aged vinegar.

本研究旨在调查红曲陈醋传统发酵过程中的微生物演替和代谢动态，探索与风味成分形成密切相关的核心功能微生物。微生物组分析表明，乳酸杆菌、醋酸杆菌、芽孢杆菌、肠杆菌、乳球菌、白念珠菌和魏氏菌是主要的细菌属，而琵琶曲霉、黑曲霉、紫云英曲霉、雅典念珠菌、木霉、赭青霉和高岛辛霉是主要的真菌属。相关性分析表明，醋酸菌与四甲基吡嗪、乙酰丙酮和醋酸的产量呈正相关，乳球菌与 2-壬酮、2-庚酮、辛酸乙酯、癸酸乙酯、1-己醇、1-辛醇和 1-辛烯-3-醇的产量呈正相关，C.xylopsoci 和 C. rugosa 与丙二酸二乙酯、2,3-丁二酯二乙酸酯、乙醛、苯甲醛和四甲基吡嗪的产生呈正相关。相应地，还通过超高效液相色谱-四极杆飞行时间质谱法检测到了非挥发性代谢物。在红曲陈醋的传统酿造过程中，发现了多种氨基酸和功能二肽。相关性分析表明，乳酸杆菌与 DL-乳酸、吲哚乳酸、D-(+)-3-苯基乳酸、匹梅酸、普瑞巴林酸和 3-氨基丁酸有显著相关性。这项研究有助于了解风味形成的机理，并为选择合适的菌株制定有效的策略，以提高红曲陈醋的风味质量。

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引用次数: 0

Lignans are the main active components of Schisandrae Chinensis Fructus for liver disease treatment: a review 木酚素是五味子果实治疗肝病的主要活性成分：综述

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250200

As a traditional Chinese herbal medicine, Schisandrae Chinensis Fructus (SC) has been used in medicine and food industry due to its health care and therapeutic effects. Over the past 20 years, the use of SC and its active ingredient lignans in the prevention and treatment of liver diseases has been increasing, and their hepatoprotective effects has increased the interest of the public and academia. Therefore, in the present work, we first determined the effectiveness of SC in the treatment of liver diseases such as metabolic associated fatty liver disease, alcoholic liver disease, cholestatic liver disease and acute liver injury. Subsequently, the pharmacological effects and molecular mechanisms of lignans, the active components of SC, for liver disease treatment were comprehensively summarized for the first time. The results showed that the lignans in SC could achieve hepatoprotective effects by regulating lipid metabolism, anti-fibrosis, anti-inflammation, anti-oxidation, anti-tumor and regulating bile acid metabolism. The mechanism mainly involved adenosine 5'-monophosphate-activated protein kinase, endoplasmic reticulum stress, sterol regulatory element binding protein 1c, autophagy, transforming growth factor-β, mitogen-activated protein kinase, microRNA, nuclear factor kappa-B, nuclear factor erythroid-2-related factor 2, heat shock proteins and pregnane X receptor signaling pathways. These results can lay a scientific foundation for the development of hepatoprotective drugs or functional foods from SC/lignans.

作为一种传统中药材，五味子（SC）因其保健和治疗作用一直被用于医药和食品行业。近 20 年来，五味子及其有效成分木脂素在预防和治疗肝脏疾病方面的应用越来越多，其保肝作用也越来越受到公众和学术界的关注。因此，在本研究中，我们首先确定了 SC 对代谢相关性脂肪肝、酒精性肝病、胆汁淤积性肝病和急性肝损伤等肝病的治疗效果。随后，首次全面总结了木质素（SC 的活性成分）治疗肝病的药理作用和分子机制。结果表明，SC 中的木质素可通过调节脂质代谢、抗纤维化、抗炎、抗氧化、抗肿瘤和调节胆汁酸代谢等作用达到保肝护肝的效果。其机制主要涉及腺苷-5'-单磷酸激活的蛋白激酶、内质网应激、甾醇调节元件结合蛋白1c、自噬、转化生长因子-β、丝裂原活化蛋白激酶、微RNA、核因子卡巴-B、核因子红细胞-2相关因子2、热休克蛋白和孕烷X受体信号通路。这些结果可为利用 SC/木质素开发保肝药物或功能食品奠定科学基础。

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引用次数: 0

Oxidative stress mediates glycidol-induced endothelial injury and its protection by 6-C-(E-2-fluorostyryl)naringenin 氧化应激介导缩水甘油诱导的内皮损伤及其 6-C-（E-2-氟苯乙烯基）柚皮素的保护作用

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250207

Glycidol is a common lipid-derived foodborne toxicant mainly presents in refined oils and related foodstuffs. Vascular endothelial cells may be potential targets of the deleterious effects associated with glycidol exposure. In human umbilical vein endothelial cells (HUVECs), we found that glycidol treatment promoted endothelial-to-mesenchymal transition (EndMT) at a lower concentration (0.5 mmol/L), while induced apoptosis and inflammation at a higher concentration (1 mmol/L). These harmful effects were achieved by the activation of NF-κB/MAPK signaling pathway and were mediated by reactive oxygen species (ROS). In addition, the protective potential of 6-C-(E-2-fluorostyryl)naringenin (6-CEFN) against glycidol was evaluated and compared with naringenin. HUVECs pre-treated with 6-CEFN, but not naringenin, displayed resistance to endothelial dysfunction caused by glycidol.

缩水甘油是一种常见的脂类食源性毒物，主要存在于精炼油和相关食品中。血管内皮细胞可能是与接触缩水甘油相关的有害效应的潜在目标。我们发现，在人脐静脉内皮细胞（HUVECs）中，较低浓度（0.5 mmol/L）的缩水甘油可促进内皮细胞向间质转化（EndMT），而较高浓度（1 mmol/L）的缩水甘油可诱导细胞凋亡和炎症。这些有害影响是通过激活 NF-κB/MAPK 信号通路和活性氧（ROS）介导实现的。此外，还评估了 6-C-（E-2-氟苯乙烯基）柚皮素（6-CEFN）对缩水甘油的保护潜力，并与柚皮素进行了比较。经 6-CEFN 而非柚皮苷预处理的 HUVECs 能抵抗缩水甘油引起的内皮功能障碍。

引用次数: 0

Dietary L-proline supplementation ameliorates autism-like behaviors and modulates gut microbiota in the valproic acid-induced mouse model of autism spectrum disorder 在丙戊酸诱导的自闭症谱系障碍小鼠模型中，补充 L-脯氨酸可改善自闭症样行为并调节肠道微生物群

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250234

The effective intervention strategy for autism spectrum disorder (ASD) are currently limited. Herein, we attempted to evaluate the potential of L-proline (Pro), a multifunctional amino acid, in ameliorating autism-like behaviors and clarify the molecular mechanisms involved by using the typical valproic acid (VPA)-induced mouse model of ASD. Pro significantly attenuates repetitive behaviors and social dysfunction in ASD mice. The correlation analysis revealed that the beneficial effects of Pro on autism-like behaviors are related to the modulation of gut microbiota structure and composition. The histological analysis revealed that Pro could reverse the decrease of Nissl-positive cells in the prefrontal cortex (PFC) induced by VPA exposure. RNA sequencing demonstrated that Pro can also alter the PFC transcriptomic profile distinguished by the regulation of genes involved in Parkinson disease, neuroactive ligand-receptor interaction, oxidative phosphorylation, and mitogen activated protein kinase signaling pathway. Overall, dietary Pro supplementation may be a promising intervention strategy for ASD.

目前，针对自闭症谱系障碍（ASD）的有效干预策略还很有限。在此，我们尝试使用典型的丙戊酸（VPA）诱导的 ASD 小鼠模型，评估多功能氨基酸 L-脯氨酸（Pro）在改善类似自闭症行为方面的潜力，并阐明其中的分子机制。Pro 能明显减轻 ASD 小鼠的重复行为和社交功能障碍。相关分析表明，Pro 对自闭症样行为的有益影响与肠道微生物群结构和组成的调节有关。组织学分析表明，Pro能逆转VPA暴露引起的前额叶皮层（PFC）Nissl阳性细胞的减少。RNA 测序表明，Pro 还能改变前额叶皮质的转录组特征，具体表现为调节涉及帕金森病、神经活性配体与受体相互作用、氧化磷酸化和丝裂原活化蛋白激酶信号通路的基因。总之，膳食中补充 Pro 可能是一种很有前景的 ASD 干预策略。

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