Food Science and Human Wellness最新文献

Identification and the molecular mechanism of novel duck liver-derived anti-inflammatory peptides in lipopolysaccharide-induced RAW264.7 cell model 在 LPS 诱导的 RAW 264.7 细胞模型中鉴定新型鸭肝源性抗炎肽及其分子机制

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250041

Xiankang Fan , Laidi Zhang , Yangying Sun , Changyu Zhou , Qiang Xia , Lihui Du , Zhen Wu , Daodong Pan

In this study, 10 novel anti-inflammatory peptides were identified from duck liver, and their molecular mechanism was demonstrated based on machine learning and molecular docking. Using Sephadex G-15 gel chromatography separation, reversed-phase high-performance liquid chromatography purification, liquid chromatography-tandem mass spectrometry identification, and BIOPEP database comparison, 10 novel anti-inflammatory peptides were initially found. Their splendid angiotensin-converting enzyme (ACE) inhibition and anti-inflammatory properties were confirmed by machine learning. With binding energies less than –20.93 kJ/mol, molecular docking revealed that they could efficiently bind to the active pockets of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), cyclooxygenase 2 (COX-2), and nuclear factor κB (NF-κB) proteins with efficiency, indicating that the compounds can spontaneously form complexes through hydrogen bonding and hydrophobic interactions with the protein binding pockets. In the lipopolysaccharide-induced RAW264.7 cell model, the release of NO, TNF-α, and IL-6 and the mRNA expression of inflammatory factors (TNF-α, IL-6, COX-2, and NF-κB) were significantly inhibited by these peptides. We concluded it might be due to their anti-inflammatory effects by inhibiting the protein phosphorylation of inhibitor of NF-κB (IκBα) in the cytoplasm and preventing the translocation of NF-κB p65 in the cytoplasm to the nucleus, thereby regulating the NF-κB signaling pathway. This study is essential for the screening of anti-inflammatory peptides and the investigation of the mechanism of action.

本研究从鸭肝中鉴定出10种新型抗炎肽，并基于机器学习和分子对接技术对其分子机制进行了论证。采用Sephadex G-15凝胶色谱分离、反相高效液相色谱纯化、液相色谱-串联质谱鉴定、BIOPEP数据库比对等方法，初步发现10种新型抗炎肽。机器学习证实了它们对血管紧张素转换酶（ACE）的抑制和抗炎作用。结合能小于-20.93 kJ/mol，分子对接发现它们能高效结合肿瘤坏死因子α (TNF-α)、白细胞介素6 （IL-6）、环氧化酶2 （COX-2）和核因子κB （NF-κB）蛋白的活性囊，表明化合物可通过与蛋白结合囊的氢键和疏水相互作用自发形成配合物。在脂多糖诱导的RAW264.7细胞模型中，这些多肽显著抑制NO、TNF-α、IL-6的释放以及炎症因子TNF-α、IL-6、COX-2、NF-κB mRNA的表达。我们推测其抗炎作用可能是通过抑制胞质中NF-κB抑制剂（i -κB α）的蛋白磷酸化，阻止胞质中NF-κB p65向细胞核的易位，从而调控NF-κB信号通路。本研究对抗炎肽的筛选和作用机制的研究具有重要意义。

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引用次数: 0

Dendrobium officinale flowers flavonoids enriched extract protects against acute ethanol-induced gastric ulcers via AMPK/PI3K signaling pathways 铁皮石斛花黄酮类富集提取物通过 AMPK/PI3K 信号通路防止乙醇诱发的急性胃溃疡

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250048

Zhiyu Zhang , Hualing Xie , Mohamed A. Farag , Zhenhao Li , Qingping Wu , Ping Shao

Gastric ulcer is a widespread disease caused by various etiologies. Dendrobium officinale flowers exert several health benefits owing to their rich flavonoid content. In this study, protective effects and possible action mechanisms of D. officinale flowers’ flavonoid enriched extract (DOFF) were assessed against gastric ulcer. The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34 % of the total extractive was flavonoid, and ultra-high performance liquid chromatography time of flight mass spectrometer (UPLC-Q-TOF/MS) revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids. In addition, in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions, with drop-in ulcer index from 64.33 ± 8.76 to 32.00 ± 4.47. Similar results were revealed in human gastric mucosal epithelia cells, with cells viability to increase from 27.2 % to 61.6 % post DOFF administration. To analyzed the protect effect of DOFF, we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways. The results showed that DOFF (320 μg/mL) could increase the level of oxidation marker protein (HO-1), apoptosis regulatory protein (Bcl-2) and autophagy marker (LC3β) by 50.84 %, 43.85 %, and 59.21 % compared with ethanol-treated group respectively. Further analyzed of the mitochondrial activity and apoptosis pathway, we found that DOFF appeared to mitigate against ethanol-induced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.

胃溃疡是一种由多种病因引起的广泛疾病。铁皮石斛花由于其丰富的类黄酮含量而具有多种健康益处。本研究探讨了officinale花黄酮富集提取物（DOFF）对胃溃疡的保护作用及其可能的作用机制。亚硝酸盐钠-硝酸铝比色法测定结果显示，总黄酮含量为52.34%；超高效液相色谱-飞行时间质谱（UPLC-Q-TOF/MS）测定结果显示，总黄酮中含有28种成分，其中14种为黄酮类成分。此外，体内实验显示，DOFF具有减少乙醇诱导的胃粘膜病变形成的潜力，溃疡指数从64.33±8.76降至32.00±4.47。在人胃粘膜上皮细胞中也发现了类似的结果，给药后细胞存活率从27.2%增加到61.6%。为了分析DOFF的保护作用，我们采用Western blotting和免疫荧光法检测了细胞通路中关键蛋白的表达水平。结果表明，与乙醇处理组相比，320 μg/mL的DOFF可使氧化标志蛋白（HO-1）、凋亡调节蛋白（Bcl-2）和自噬标志蛋白（LC3β）水平分别提高50.84%、43.85%和59.21%。进一步分析线粒体活性和凋亡通路，我们发现DOFF似乎通过AMPK/mTOR/ULK1和PI3K/AKT自噬信号通路减轻乙醇诱导的胃粘膜损伤。

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引用次数: 0

Co-inoculation of Debaryomyces hansenii and lactic acid bacteria: a strategy to improve the taste and odour profiles of dry sausages 汉森德氏酵母菌和乳酸菌的联合接种：改善干香肠口感和气味的策略

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250014

Rongxin Wen , Yumeng Sui , Jiaqi Liu , Huiping Wang , Baohua Kong , Ligang Qin , Qian Chen

The effects of the co-inoculation of Debaryomyces hansenii separately with 3 lactic acid bacteria (LAB), Lactobacillus sakei, Lactobacillus plantarum and Lactobacillus curvatus, on the taste and odour profiles of dry sausages were investigated. The co-inoculated sausages showed higher free amino acid and organic acid contents than the non-inoculated control and sausages inoculated with D. hansenii alone. Meanwhile, the sausages inoculated with D. hansenii + L. plantarum, D. hansenii + L. sakei and D. hansenii + L. curvatus had the highest contents of aldehydes, esters and alcohols, respectively. The results of electronic tongue, electronic nose and sensory evaluation demonstrated that compared with the sausage inoculated with D. hansenii, the sour taste and floral odour increased and the fatty odour decreased in the sausage inoculated with D. hansenii + L. sakei; this was more favourable for the development of a desirable flavour in sausages. Moreover, the partial least squares regression analysis indicated that 10 taste and 33 odour compounds were mainly responsible for the differences in the flavour profiles among the sausages. Overall, these findings contributed to a more comprehensive understanding of the formation of sensory characteristics in dry sausages co-inoculated with yeast and LAB.

研究了汉斯德巴氏菌与酒井乳杆菌、植物乳杆菌和弯曲乳杆菌3种乳酸菌共接种对干香肠口感和气味的影响。共接种香肠的游离氨基酸和有机酸含量高于未接种对照和单独接种香肠。与此同时，大肠杆菌+植物乳杆菌、大肠杆菌+ sakaki乳杆菌和大肠杆菌+弯曲乳杆菌接种的香肠中醛类、酯类和醇类含量最高。电子舌、电子鼻和感官评价结果表明，与接种了猪肠杆菌的香肠相比，接种了猪肠杆菌的香肠酸味和花香增加，脂肪味减少；这更有利于香肠中理想风味的发展。此外，偏最小二乘回归分析表明，10种味道化合物和33种气味化合物是香肠风味特征差异的主要原因。总的来说，这些发现有助于更全面地了解酵母和乳酸菌共接种干香肠的感官特征的形成。

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引用次数: 0

Soy polysaccharide maintains colonic homeostasis to protect from dextran sulphate sodium-induced colitis by modulating gut microbiota and intestinal epithelial regeneration 大豆多糖通过调节肠道微生物群和肠上皮再生，维持结肠稳态，防止右旋糖酐硫酸钠诱发结肠炎

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250015

Nana Zhang , Jianlin Liu , Weiyue Zhang , Xinxin Guo , Shuying Li , Hongtai Zhang , Minjie Wang , Bei Fan , Fengzhong Wang

Soy polysaccharide (SP) has been reported to possess the properties of modulating gut microbiome diversity. Here, we aimed to explore the protective effects of SP against dextran sulphate sodium (DSS)-induced colitis. Pre-treatment with SP at a dosage of 400 mg/kg·day alleviated colitis symptoms, preventing the weight loss and colon shorten. SP suppressed DSS-induced inflammatory response and enhanced M1 to M2 macrophage polarization. Further investigation showed that SP significantly promoted the regeneration of crypt and the expansion of goblet cell production. In addition, bacterial 16S rRNA sequencing analysis showed that SP modulated the composition of fecal microbiota, including selectively increasing Lactobacillus relative abundance. Notably, SP treatment enriched the production of Lactobacillus-derived lactic acid, which was sensed by its specific G-protein-coupled receptor 81 (Gpr81)/Wnt3/β-catenin signaling, and promoted the regeneration of intestinal stem cells. Fecal microbiome transplantation demonstrated that intestinal flora partially contributed to the beneficial effects of SP on preventing against colitis. In conclusion, SP exhibited the protective effects against colitis, which could be partly associated with modulating the composition of gut microbiota and enrichment of lactic acid. This study suggests that SP has potential to be developed as nutritional intervention to prevent colitis.

大豆多糖（SP）已被报道具有调节肠道微生物多样性的特性。在这里，我们旨在探讨SP对葡聚糖硫酸钠（DSS）诱导的结肠炎的保护作用。预用药剂量为400 mg/kg·d的SP可缓解结肠炎症状，防止体重下降和结肠缩短。SP抑制dss诱导的炎症反应，增强巨噬细胞M1 - M2极化。进一步研究表明，SP显著促进了隐窝细胞的再生和杯状细胞的扩增。此外，细菌16S rRNA测序分析表明，SP调节了粪便微生物群的组成，包括选择性地增加乳酸杆菌的相对丰度。值得注意的是，SP处理丰富了乳酸菌源性乳酸的产生，这是通过其特异性g蛋白偶联受体81 (Gpr81)/Wnt3/β-catenin信号传导来感知的，并促进了肠道干细胞的再生。粪便微生物组移植表明，肠道菌群部分参与了SP预防结肠炎的有益作用。综上所述，SP对结肠炎具有保护作用，可能与调节肠道菌群组成和乳酸富集有关。本研究提示SP有潜力作为预防结肠炎的营养干预措施。

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引用次数: 0

Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy 通过改善肠道屏障和激活肠道自噬来保护黑腹果蝇免受麦角硫因治疗的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250028

Hongyu Pan , Qianwang Zheng , Yuan Zou , Guozhao Luo , Mingzhen Tu , Na Wang , Jingru Zhong , Liqiong Guo , Junfang Lin

Ergothioneine is a natural amino acid with powerful antioxidant activity. Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans, and enhance telomerase activity. In present study, the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies. And the alterations in the gut barrier, gut morphology, and other gut physiological indicators of aging flies were evaluated. Here, the results showed that ergothioneine induced little variations in gut microbiota composition in flies at mid-life, and significant alterations in the gut microbiota populations were only observed in flies at late-life. And ergothioneine attenuated age-related gut injuries, including decreasing reactive oxygen species levels in intestinal enterocytes, reduction of intestinal cells death, and preservation of gut integrity in aging flies. Ergothioneine also improved the gut morphology by preserving the structure and shape of cell organelles and inducing the intestinal autophagy. And activation of intestinal autophagy also contributed to the longevity effect mediated by ergothioneine in an unidentified mechanism.

麦角硫因是一种天然氨基酸，具有强大的抗氧化活性。先前的研究表明麦角硫因可以延长黑胃果蝇和秀丽隐杆线虫的寿命，并提高端粒酶的活性。本研究采用宏基因组测序和实时定量聚合酶链反应分析对衰老果蝇肠道微生物组的组成和多样性进行了表征。并对衰老果蝇肠道屏障、肠道形态及其他肠道生理指标的变化进行了评价。本研究的结果表明，麦角硫因对中年果蝇肠道菌群组成的影响很小，仅在晚年果蝇中观察到肠道菌群的显著变化。麦角硫因减轻了衰老果蝇中与年龄相关的肠道损伤，包括降低肠道肠细胞中的活性氧水平、减少肠道细胞死亡和保持肠道完整性。麦角硫因还通过保持肠道细胞器的结构和形状，诱导肠道自噬来改善肠道形态。肠道自噬的激活也有助于麦角硫因介导的长寿效应，其机制尚不清楚。

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引用次数: 0

Nostoc commune Vauch. polysaccharide alleviates Pb-induced hepatorenal toxicity by changing gut bacteriome, mycobiome, and metabolome Nostoc commune Vauch.多糖通过改变肠道细菌组、霉菌生物组和代谢组减轻铅诱导的肝肾毒性

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250032

Yue Liu , Ping Ren , Hailong Li , Yingying Liu , Yiting Yang , Wanting Wang , Jiaming Zhou , Ling Su , Lili Guan , Hongxia Ma

This study aimed to explore the protective effect and potential mechanism of Nostoc commune Vauch. polysaccharide (NCVP) on lead (Pb)-poisoning mice. NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses and modulated key indicators of antioxidant capacity. Moreover, the down-regulation of critical proteins of the Nrf2 pathway induced by Pb could be reversed after NCVP intervention. In addition, NCVP maintained the diversity of gut bacteriobiota and restored the relative abundance of f_Prevotellaceae, g_Alloprevotella, and f_ Eubacterium_coprostanoligenes_group reduced by Pb. Also, NCVP regulated the diversity and abundance of gut mycobiota affected by Pb. Specifically, Pb decreased the proportion of pathogenic species (g_Fusarium, p_Basidiomycota, g_Alternaria, g_Aspergillus, and g_Candida) while NCVP increased the abundance of probiotics species (g_Kazachstania and p_Ascomycota). Furthermore, the metabolomic analysis found that NCVP significantly altered a range of microbial metabolites, including porphobilinogen, cromakalim, salidroside, and trichostatin A, which has significant associations with specific gut bacteriobiota or mycobiota. These altered metabolites are involved in primary bile acid biosynthesis, metabolism of xenobiotics by cytochrome P450, lysine degradation, and other metabolic pathways. Overall, our findings indicate that NCVP might be an excellent natural product for eliminating Pb-induced hepatorenal toxicity, possibly by regulating gut bacteriome, mycobiome and metabolome.

本研究旨在探讨野藿香的保护作用及其潜在机制。多糖（NCVP）对铅中毒小鼠的影响。NCVP改善了铅诱导的肝肾毒性和炎症反应，并调节了抗氧化能力的关键指标。此外，NCVP干预后，Pb诱导的Nrf2通路关键蛋白的下调可以逆转。NCVP维持了肠道菌群的多样性，恢复了被Pb减少的f_Prevotellaceae、g_Alloprevotella和f_ Eubacterium_coprostanoligenes_group的相对丰度。NCVP还能调节受铅影响的肠道菌群的多样性和丰度。其中，Pb降低了病原菌种类（g_Fusarium、p_担子菌、g_Alternaria、g_Aspergillus和g_Candida）的比例，NCVP增加了益生菌种类（g_Kazachstania和p_Ascomycota）的丰度。此外，代谢组学分析发现，NCVP显著改变了一系列微生物代谢物，包括卟啉原、cromakalim、红景天苷和trichostatin a，这些代谢物与特定的肠道菌群或真菌群有显著关联。这些改变的代谢物参与初级胆汁酸生物合成、细胞色素P450对异种生物的代谢、赖氨酸降解和其他代谢途径。总之，我们的研究结果表明，NCVP可能是消除铅诱导的肝肾毒性的一种极好的天然产物，可能通过调节肠道细菌组、真菌组和代谢组来消除铅诱导的肝肾毒性。

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引用次数: 0

Effects of dietary olive oil, camellia seed oil and soybean oil on serum lipid composition in women with a high risk of cardiovascular disease: a lipidomic analysis 膳食橄榄油、山茶籽油和大豆油对心血管疾病高危女性血清脂质组成的影响：脂质组分析

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250006

Minyu Wu , Changfeng Hu , Lirong Shen

Numerous studies currently compare the lipid metabolism in patients with cardiovascular disease (CVD) and healthy individuals to identify lipid markers for predicting CVD. In this study, multidimensional mass spectrometry-based shotgun lipidomics was used to examine the serum lipidomics of participants in a clinical randomized controlled feeding trial undergoing olive oil (OO), camellia seed oil (CSO), and soybean oil (SO) dietary interventions. 189 lipid molecules are identified, including 14 species of phosphatidylinositol, 45 species of ethanolamine glycerols (PE), 47 species of choline glycerophospholipids (PC), 39 species of triacylglycerols (TAG), 18 species of lysophosphatidylcholine, and 26 species of sphingomyelin. After screening, 10 lipid markers are found, among which 18:2 fatty acid (FA), 16:1 FA, C_54:4/C_55:11, C_54:3/C_55:10, and C_52:3/C_53:10 in TAG pool, p_18:0/20:0 and a_18:0/18:1 in PC pool, and p_18:1/20:4 in PE pool have differential regulation in the SO group compared to OO and CSO. The d_16:0/18:1 in PC pool and C_52:2/C_53:9 in TAG pool are differentially regulated by OO and CSO. The C_52:2/C_53:9 in TAG pool has a significant negative correlation with aspartate aminotransferase (r = -0.363, P = 0.048) and high-density lipoprotein cholesterol (r = -0.519, P < 0.01). This study provides a reference for researching the effect of dietary fat on blood lipid metabolism.

目前，许多研究比较了心血管疾病（CVD）患者和健康个体的脂质代谢，以确定预测CVD的脂质标志物。在这项研究中，基于多维质谱的霰弹枪脂质组学被用于检测一项临床随机对照喂养试验参与者的血清脂质组学，这些参与者接受橄榄油（OO）、茶籽油（CSO）和大豆油（SO）的饮食干预。共鉴定出189种脂类分子，其中磷脂酰肌醇14种，乙醇胺甘油（PE） 45种，胆碱甘油磷脂（PC） 47种，三酰基甘油（TAG） 39种，溶血磷脂18种，鞘磷脂26种。经筛选，发现10个脂质标志物，其中TAG组18:2脂肪酸（FA）、16:1脂肪酸、C54:4/C55:11、C54:3/C55:10、C52:3/C53:10、PC组p18:0/20:0、a18:0/18:1、PE组p18:1/20:4在SO组与OO、CSO组有差异调节。PC池中的d16:0/18:1和TAG池中的C52:2/C53:9受到OO和CSO的不同调节。TAG池C52:2/C53:9与天冬氨酸转氨酶（r = -0.363, P = 0.048）、高密度脂蛋白胆固醇(r = -0.519, P <；0.01)。本研究为研究膳食脂肪对血脂代谢的影响提供了参考。

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引用次数: 0

The impact of the novel starch-lipid complexes on the glucolipids metabolism, inflammation, and gut dysbiosis of type 2 diabetes mellitus rats 新型淀粉-脂质复合物对 2 型糖尿病大鼠糖脂代谢、炎症和肠道菌群失调的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250008

Yingshuang Lu , Renbing Qin , Jin Wang , Fengjuan Xiang , Yan Zhang , Bowei Zhang , Dancai Fan , Huan Lü , Xuemeng Ji , Shujun Wang , Shuo Wang

It has been widely accepted that resistant starch (RS) provides numerous health benefits for human. In this research, we aimed at evaluating the performance of novel starch-lipid complexes, RS5, in comparison with RS2 on physical features, glucolipids metabolism, inflammation, and gut microbiota profiles of type 2 diabetes mellitus (T2DM) rats. The T2DM model was established by streptozotocin injection to the high-fat-sugar fed rats. According to a serial of biochemical analyses, we found that RS5 diets were strongly correlated with enhanced homeostatic model assessment for insulin secretion (HOMA-IS), high-density lipoprotein cholesterol (HDL-C), adiponectin (ADP), insulin action index (IAI), glucagon-like peptide-1 (GLP1), and short-chain fatty acids (SCFAs) in T2DM rats whilst negatively associated with the low-density lipoprotein (LDL-C) and inflammatory cytokines, showing the capabilities to ameliorate T2DM symptoms by regulation of glucolipid metabolism, gut metabolites, and inflammation. On the other hand, RS2-enriched supplementations were influential in the mediation of insulin secretion to improve glucose metabolism. The increasing evidence collected herein suggested that intestinal microbiota could mediate glucolipids metabolism and alleviate inflammation after certain microflora nourished by RS. In addition, RS intake made an impact on phosphoinositide 3-kinase/protein kinase B signaling pathway that might contribute to the improvement of glucose metabolism, insulin resistance, and inflammatory responses.

抗性淀粉（RS）为人类健康提供了许多益处，这已被广泛接受。在这项研究中，我们旨在评估新型淀粉-脂质复合物RS5与RS2在2型糖尿病（T2DM）大鼠的身体特征、糖脂代谢、炎症和肠道微生物群方面的性能。采用链脲佐菌素注射高脂糖喂养大鼠建立T2DM模型。根据一系列生化分析，我们发现RS5饮食与T2DM大鼠胰岛素分泌（HOMA-IS）、高密度脂蛋白胆固醇（HDL-C）、脂联素（ADP）、胰岛素作用指数（IAI）、胰高血糖素样肽-1 （GLP1）和短链脂肪酸（SCFAs）增强的稳态模型评估密切相关，而与低密度脂蛋白（LDL-C）和炎症因子呈负相关。显示通过调节糖脂代谢、肠道代谢物和炎症改善T2DM症状的能力。另一方面，富含rs2的补品可介导胰岛素分泌，改善葡萄糖代谢。越来越多的证据表明，肠道菌群在被RS营养后可以介导糖脂代谢，减轻炎症反应，并且RS摄入对磷酸肌肽3-激酶/蛋白激酶B信号通路的影响可能有助于改善糖代谢、胰岛素抵抗和炎症反应。

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引用次数: 0

Food-derived peptides with inhibitory capacity for HMG-CoA reductase activity: a potential nutraceutical for hypercholesterolemia 具有 HMG-CoA 还原酶活性抑制能力的食品肽：治疗高胆固醇血症的潜在营养保健品

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250001

Guillermo Santos-Sánchez , Ana Isabel Álvarez-López , Eduardo Ponce-España , Patricia Judith Lardone , Antonio Carrillo-Vico , Ivan Cruz-Chamorro

Cardiovascular diseases (CVDs) are the leading global cause of mortality and disease burden. Statins are the most prescribed lipid-lowering drugs to treat hypercholesterolemia and prevent CVDs. The biochemical mechanism of statins consists of competitive inhibition of the 3-hydroxy-3-methylglutaryl-coenzyme A reductase enzyme (HMG-CoAR), the limiting enzyme in cholesterol biosynthesis. Due to statin intolerance in some patient groups, the search for new inhibitors is a field of great interest. This review focusses on the studies reporting the inhibitory effect of protein hydrolysates and biopeptides on the HMG-CoAR enzyme activity. The analysis of the action mechanism and physicochemical characteristics of the HMG-CoAR inhibitory peptides revealed that the molecular weight, amino acid composition, charge, and polarity are key aspects of the interaction with the HMG-CoAR enzyme. In conclusion, this review reveals the potential of using food peptides as new cholesterol-lowering agents and opens a new interesting field of research. However, clinical approaches are mandatory to confirm their therapeutic hypercholesterolemic effect.

心血管疾病（cvd）是导致死亡和疾病负担的主要全球原因。他汀类药物是治疗高胆固醇血症和预防心血管疾病最常用的降脂药物。他汀类药物的生化机制是竞争性抑制3-羟基-3-甲基戊二酰辅酶A还原酶（HMG-CoAR），而HMG-CoAR是胆固醇生物合成的限制性酶。由于他汀类药物不耐受在一些患者群体中，寻找新的抑制剂是一个非常感兴趣的领域。本文综述了蛋白水解物和生物多肽对HMG-CoAR酶活性抑制作用的研究进展。对HMG-CoAR抑制肽的作用机理和理化特性分析表明，分子量、氨基酸组成、电荷和极性是影响HMG-CoAR酶相互作用的关键因素。综上所述，本文综述揭示了利用食物肽作为新型降胆固醇剂的潜力，开辟了一个新的研究领域。然而，临床方法是强制性的，以确认其治疗高胆固醇血症的效果。

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引用次数: 0

Dietary (poly)phenol intake is associated with cardiometabolic health parameters in adolescents 膳食（多）酚摄入量与青少年心脏代谢健康参数有关

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-11-01 DOI: 10.26599/FSHW.2023.9250023

Emily P. Laveriano-Santos , Sara Castro-Barquero , Camila Arancibia-Riveros , Ana María Ruiz-León , Rosa Casas , Ramón Estruch , Patricia Bodega , Mercedes de Miguel , Amaya de Cos-Gandoy , Jesús Martínez-Gómez , Gloria Santos-Beneit , Juan M. Fernández-Alvira , Rodrigo Fernández-Jiménez , Rosa M. Lamuela-Raventós , Anna Tresserra-Rimbau

The protective role of (poly)phenols against metabolic disorders has been extensively studied in adults but not in adolescents. To assess associations of dietary (poly)phenols and their subclasses with cardiometabolic health parameters in adolescents. A cross-sectional study was conducted in 944 individuals aged 11–14 years enrolled in the SI! Program for Secondary Schools trial (NCT03504059). (Poly)phenol intake was assessed using semi-quantitative food frequency questionnaires and the Phenol-Explorer database. The measured cardiometabolic parameters were waist circumference (WC) age-sex Z-score, blood pressure (BP) age-sex Z-score, blood glucose (BG), triglycerides (TG), and high-density lipoprotein cholesterol (HDL-c). Multilevel mixed-effect linear regression models were applied to examine the association between (poly)phenol quintiles and cardiometabolic health parameters. Compared to the lowest quintile, adolescents in the highest quintile of total (poly)phenol intake had lower WC Z-scores, mean arterial pressure Z-scores, and HDL-c after multivariable adjustment. The WC Z-scores and HDL-c were lower in the highest quintile of flavonoid intake compared to the lowest quintile. The highest quintile of phenolic acid intake was associated with a lower WC Z-score and TG levels, and the highest quintile of stilbene intake with lower BG and TG, and with higher HDL-c compared to the lowest quintile. A higher intake of (poly)phenols, especially flavonoids, phenolic acids, and stilbenes, was associated with better cardiometabolic parameters in adolescents.

（多）酚对代谢紊乱的保护作用已在成人中广泛研究，但在青少年中尚未研究。评估青少年饮食（多）酚及其亚类与心脏代谢健康参数的关系。一项横断面研究对944名11-14岁的SI！中学项目试验（NCT03504059）。采用半定量食物频率问卷和酚- explorer数据库评估多酚摄入量。测量的心脏代谢参数为腰围（WC）、年龄-性别Z-score、血压（BP）、年龄-性别Z-score、血糖（BG）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-c）。采用多水平混合效应线性回归模型检验（多）酚五分位数与心脏代谢健康参数之间的关系。与最低的五分位数相比，总（多）酚摄入量最高的五分位数的青少年在多变量调整后的WC z评分、平均动脉压z评分和HDL-c均较低。在类黄酮摄入量最高的五分位数中，WC - z分数和HDL-c低于最低的五分位数。与最低五分位数相比，酚酸摄入量最高的五分位数与较低的WC - z评分和TG水平相关，而苯乙烯摄入量最高的五分位数与较低的BG和TG以及较高的HDL-c水平相关。青少年摄入较多的（多）酚类物质，特别是类黄酮、酚酸和二苯乙烯，与较好的心脏代谢参数有关。

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引用次数: 0