Food Science and Human Wellness最新文献

Saikosaponin D improves non-alcoholic fatty liver disease via gut microbiota-bile acid metabolism pathway 柴胡皂苷 D 通过肠道微生物群-胆汁酸代谢途径改善非酒精性脂肪肝

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250218

Non-alcoholic fatty liver disease (NAFLD) is the main cause of chronic liver disease worldwide. Bupleurum is widely used in the treatment of non-alcoholic fatty liver, and saikosaponin D (SSD) is one of the main active components of Bupleurum. The purpose of this study was to investigate the efficacy of SSD in the treatment of NAFLD and to explore the mechanism of SSD in the improvement of NAFLD based on “gut-liver axis”. Our results showed that SSD dose-dependently alleviated high fat diet-induced weight gain in mice, improved insulin sensitivity, and also reduced liver lipid accumulation and injury-related biomarkers aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Further exploration found that SSD inhibited the mRNA expression levels of farnesoid X receptor (Fxr), small heterodimer partner (Shp), recombinant fibroblast growth factor 15 (Fgf15) and apical sodium dependent bile acid transporter (Asbt) in the intestine, suggesting that SSD improved liver lipid metabolism by inhibiting intestinal FXR signaling. SSD can significantly reduce the gut microbiota associated with bile salt hydrolase (BSH) expression, such as Clostridium. Decreased BSH expression reduced the ratio of unconjugated to conjugated bile acids, thereby inhibiting the intestinal FXR. These data demonstrated that SSD ameliorated NAFLD potentially through the gut microbiota-bile acid-intestinal FXR pathway and suggested that SSD is a promising therapeutic agent for the treatment of NAFLD.

非酒精性脂肪肝是全球慢性肝病的主要病因。柴胡被广泛用于治疗非酒精性脂肪肝，而柴胡皂苷 D（SSD）是柴胡的主要活性成分之一。本研究旨在探讨柴胡皂苷 D 治疗非酒精性脂肪肝的疗效，并探索基于 "肠肝轴 "的柴胡皂苷 D 改善非酒精性脂肪肝的机制。研究结果表明，SSD能剂量依赖性地缓解高脂饮食诱导的小鼠体重增加，改善胰岛素敏感性，还能减少肝脏脂质蓄积和损伤相关的生物标志物天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）。进一步研究发现，SSD 可抑制肠道中法尼类固醇 X 受体（Fxr）、小异二聚体伙伴（Shp）、重组成纤维细胞生长因子 15（Fgf15）和顶端钠依赖性胆汁酸转运体（Asbt）的 mRNA 表达水平，这表明 SSD 可通过抑制肠道 FXR 信号转导来改善肝脏脂质代谢。SSD 能显著减少与胆盐水解酶（BSH）表达相关的肠道微生物群，如梭状芽孢杆菌。BSH 表达的减少降低了非结合胆汁酸与结合胆汁酸的比例，从而抑制了肠道 FXR。这些数据表明，SSD 可通过肠道微生物群-胆汁酸-肠道 FXR 途径改善非酒精性脂肪肝，并表明 SSD 是一种治疗非酒精性脂肪肝的有效药物。

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引用次数: 0

Tetramethylpyrazine and paeoniflorin combination (TMP-PF) alleviates atherosclerosis progress by reducing hyperlipemia and inhibiting plaque angiogenesis via the NR4A1/VEGFR2 pathway 四甲基吡嗪和芍药苷复方制剂（TMP-PF）通过 NR4A1/VEGFR2 途径降低高脂血症并抑制斑块血管生成，从而缓解动脉粥样硬化的进展

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250212

Atherosclerosis remains a great threat to human health worldwide. Previous studies found that tetramethylpyrazine (TMP) and paeoniflorin (PF) combination (TMP-PF) exerts anti-atherosclerotic effects in vitro. However, whether TMP-PF improves atherosclerosis in vivo needs further exploration. The present study aims to assess the anti-atherosclerotic properties of TMP-PF in ApoE^-/- mice and explore the related molecule mechanisms. Results showed that TMP and high-dose TMP-PF decreased serum triglyceride and low-density lipoprotein cholesterol levels, suppressed vascular endothelial growth factor receptor 2 (VEGFR2) and nuclear receptor subfamily 4 group A member 1 (NR4A1) expression in aortic tissues, inhibited plaque angiogenesis, reduced plaque areas, and alleviated atherosclerosis in ApoE^-/- mice. Also, TMP-PF exhibited a better modulation effect than TMP or PF alone. However, NR4A1 agonist abolished the anti-atherosclerotic effects of TMP-PF. In conclusion, TMP-PF was first found to alleviate atherosclerosis progression by reducing hyperlipemia and inhibiting plaque angiogenesis via the NR4A1/VEGFR2 pathway, indicating that TMP-PF had a positive effect on reducing hyperlipemia and attenuating atherosclerosis development.

动脉粥样硬化仍然是全球人类健康的一大威胁。以往的研究发现，四甲基吡嗪（TMP）和芍药苷（PF）复方制剂（TMP-PF）在体外具有抗动脉粥样硬化的作用。然而，TMP-PF 是否能改善体内动脉粥样硬化还需要进一步探讨。本研究旨在评估 TMP-PF 在载脂蛋白E-/-小鼠体内的抗动脉粥样硬化特性，并探讨相关分子机制。结果显示，TMP和大剂量TMP-PF可降低载脂蛋白E-/-小鼠血清甘油三酯和低密度脂蛋白胆固醇水平，抑制血管内皮生长因子受体2（VEGFR2）和核受体亚家族4 A组1（NR4A1）在主动脉组织中的表达，抑制斑块血管生成，减少斑块面积，缓解动脉粥样硬化。此外，TMP-PF 比单独使用 TMP 或 PF 有更好的调节作用。然而，NR4A1激动剂会取消TMP-PF的抗动脉粥样硬化作用。总之，首次发现TMP-PF可通过NR4A1/VEGFR2途径降低高脂血症和抑制斑块血管生成，从而缓解动脉粥样硬化的进展，表明TMP-PF对降低高脂血症和减轻动脉粥样硬化的发展有积极作用。

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引用次数: 0

2′-Fucosyllactose alleviate immune checkpoint blockade-associated colitis by reshaping gut microbiota and activating AHR pathway 2′-岩藻糖通过重塑肠道微生物群和激活AHR通路缓解免疫检查点阻断剂相关性结肠炎

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250205

Immune checkpoint blockade (ICB) therapeutics are highly effective in cancer immunotherapy, but gastrointestinal toxicity limited the application. Intestinal microbiota plays a crucial role in ICB-associated colitis. 2′-Fucosyllactose (2′FL) is most abundance prebiotic in human milk that can reshape gut microbiota and exert immune regulatory effect. The study aimed to determine the effects of 2′FL on ICB-associated colitis and to uncover the mediating mechanism. ICB-associated colitis was induced by the ipilimumab and dextran sulfate sodium. Oral administration of 2′FL (0.6 g/(kg∙day)) ameliorated ICB-induced colitis by enhancing regulatory T cells (Treg) and the M2/M1 ratio of macrophages in colon. 2′FL treatment also increased the expression of tight junction proteins (zonula occludens-1 (ZO-1) and mucin 2 (MUC2)) and antioxidant stress indicators (superoxide dismutase (SOD) and catalase (CAT)). In addition, administration of 2′FL increased the abundance of Bifidobacterium and Lactobacillus, and elevated the levels of microbial metabolites, such as indole-3-lactic acid (ILA), which activated the aryl hydrocarbon receptor ligands (AHR) pathway. The protective effect of 2′FL was abolished upon depletion of gut microbiota, and ILA treatment partially simulated the protective effect of 2′FL. Notably, 2′FL did not exhibit inhibition of antitumor immunity. These findings suggest that 2′FL could serve as a potential protective strategy for ICB-associated colitis by modulating the intestinal microbiota and bacterial metabolites.

免疫检查点阻断（ICB）疗法在癌症免疫疗法中非常有效，但胃肠道毒性限制了其应用。肠道微生物群在 ICB 相关性结肠炎中起着至关重要的作用。2′-岩藻酸半乳糖（2′FL）是母乳中含量最丰富的益生元，可重塑肠道微生物群并发挥免疫调节作用。本研究旨在确定2′FL对ICB相关性结肠炎的影响，并揭示其介导机制。伊匹单抗和葡聚糖硫酸钠诱导了ICB相关性结肠炎。口服2′FL（0.6克/（千克/天））通过增强调节性T细胞（Treg）和结肠中巨噬细胞的M2/M1比值来改善ICB诱导的结肠炎。2′FL还能增加紧密连接蛋白（Zonula occludens-1（ZO-1）和粘蛋白2（MUC2））和抗氧化应激指标（超氧化物歧化酶（SOD）和过氧化氢酶（CAT））的表达。此外，2′FL 还能增加双歧杆菌和乳酸杆菌的数量，提高微生物代谢产物（如吲哚-3-乳酸（ILA））的水平，从而激活芳基烃受体配体（AHR）途径。消耗肠道微生物群后，2′FL 的保护作用消失，而 ILA 处理可部分模拟 2′FL 的保护作用。值得注意的是，2′FL 对抗肿瘤免疫没有抑制作用。这些研究结果表明，2′FL 可以通过调节肠道微生物群和细菌代谢产物作为一种潜在的保护性策略来治疗 ICB 相关性结肠炎。

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引用次数: 0

The metabolic profiling of Chinese yam fermented by Saccharomyces boulardii and the biological activities of its ethanol extract in vitro 布拉氏酵母菌发酵山药的代谢谱分析及其乙醇提取物的体外生物活性

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250219

Chinese yam (Dioscorea opposita Thunb.), as one of the medicinal and edible homologous plants, is rich in various nutrients and functional factors. In this study, Chinese yam fermented by Saccharomyces boulardii was performed to investigate its bioactive components and metabolic profile. And then, the main bioactive components and biological activities of fermented Chinese yam ethanol extract (FCYE) were evaluated. Results showed that there were 49 up-regulated metabolites and 52 down-regulated metabolites in fermented Chinese yam compared to unfermented Chinese yam. Besides, corresponding metabolic pathways analysis initially revealed that the distribution of bioactive substances was concentrated on alcohol-soluble small molecular substances. Ulteriorly, the total polyphenol content and the total flavonoid content in FCYE were significantly increased, and the corresponding antioxidant and immunomodulatory activities in vitro were also significantly enhanced. Our study provided a new reference for the comprehensive utilization of Chinese yam and laid a theoretical foundation for the development and application of natural probiotic-fermented products.

山药（Dioscorea opposita Thunb.）作为药食同源植物之一，富含多种营养物质和功能因子。本研究采用布拉氏酵母菌发酵山药，研究其生物活性成分和代谢概况。然后，对发酵山药乙醇提取物（FCYE）的主要生物活性成分和生物活性进行了评价。结果表明，与未发酵山药相比，发酵山药中有 49 个上调代谢物和 52 个下调代谢物。此外，相应的代谢途径分析初步显示，生物活性物质的分布主要集中在醇溶性小分子物质上。最终，发酵山药中的总多酚含量和总黄酮含量明显增加，相应的体外抗氧化活性和免疫调节活性也明显提高。我们的研究为山药的综合利用提供了新的参考，并为天然益生菌发酵产品的开发和应用奠定了理论基础。

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引用次数: 0

Insights into the enhancement of food flavor perception: strategies, mechanism and emulsion applications 洞察食品风味感知的增强：策略、机制和乳液应用

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250199

The core drivers of the modern food industry are meeting consumer demand for tasty and healthy foods. The application of food flavor perception enhancement can help to achieve the goals of salt- and sugar-reduction, without compromising the sensory quality of the original food, and this has attracted increasing research attention. The analysis of bibliometric results from 2002 to 2022 reveals that present flavor perception enhancement strategies (changing ingredient formulations, adding salt/sugar substitutes, emulsion delivery systems) are mainly carry out based on sweetness, saltiness and umami. Emulsion systems is becoming a novel research foci and development trends of international food flavor perception-enhancement research. The structured design of food emulsions, by using interface engineering technology, can effectively control, or enhance the release of flavor substances. Thus, this review systematically summarizes strategies, the application of emulsion systems and the mechanisms of action of food flavor perception-enhancement technologies, based on odor-taste cross-modal interaction (OTCMI), to provide insights into the further structural design and application of emulsion systems in this field.

现代食品工业的核心驱动力是满足消费者对美味和健康食品的需求。食品风味感知增强技术的应用有助于实现减盐减糖的目标，同时又不影响原有食品的感官质量，这已引起越来越多的研究关注。对 2002 年至 2022 年文献计量结果的分析表明，目前的风味感知增强策略（改变配料配方、添加盐/糖替代品、乳化输送系统）主要是基于甜味、咸味和鲜味进行的。乳化系统正成为国际食品风味感知增强研究的一个新的研究热点和发展趋势。利用界面工程技术对食品乳液进行结构化设计，可以有效控制或增强风味物质的释放。因此，本综述系统地总结了基于气味-味道跨模态相互作用（OTCMI）的食品风味感知增强技术的策略、乳化系统的应用和作用机理，为乳化系统在该领域的进一步结构设计和应用提供启示。

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引用次数: 0

Maternal dietary patterns associated with bone density in Chinese lactating women and infants at 6 months postpartum: a prospective study using data from 2018–2019 母亲膳食模式与中国哺乳期妇女和产后6个月婴儿骨密度的相关性：利用2018-2019年数据进行的前瞻性研究

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250214

This cohort study was designed to explore the relationship between maternal dietary patterns (DPs) and bone health in Chinese lactating mothers and infants. We recruited 150 lactating women at 1-month postpartum. The estimated bone mineral density (eBMD) of subjects’ calcanei and the information on dietary intake were collected. After 5-month follow-up, the eBMD of mothers and their infants were measured again. Factor analysis was applied to determine maternal DPs. General linear models were used to evaluate the association between maternal DPs and maternal eBMD loss or infants’ eBMD. With all potential covariates adjusted, Factor 2 (high intake of whole grains, tubers, mixed beans, soybeans and soybean products, seaweeds, and nuts) showed a positive association with the changes of maternal eBMD (β = 0.16, 95 % CI: 0.005, 0.310). Factor 3 (high intake of soft drinks, fried foods, and puffed foods) was inversely correlated with the changes of maternal eBMD (β = –0. 22, 95 % CI: -0.44, 0.00). The changes of maternal eBMD were positively associated with 6-month infants’ eBMD (β = 0.34, 95 % CI: 0.017, 0.652). In conclusion, Factor 2 might contribute to the maintenance of eBMD in lactating women, while Factor 3 could exacerbate maternal eBMD loss. Additionally, the changes of maternal eBMD presented a positive correlation with 6-month infants’ eBMD.

这项队列研究旨在探讨中国哺乳期母婴的母亲膳食模式（DPs）与骨骼健康之间的关系。我们招募了 150 名产后 1 个月的哺乳期妇女。我们收集了受试者钙骨的估计骨矿物质密度（eBMD）和膳食摄入信息。随访 5 个月后，再次测量母亲及其婴儿的 eBMD。采用因子分析来确定母亲的DP。一般线性模型用于评估母体DP与母体eBMD损失或婴儿eBMD之间的关系。在调整了所有潜在的协变量后，因子 2（全谷物、块茎类、杂豆、大豆和大豆制品、海藻类和坚果的高摄入量）与母体 eBMD 的变化呈正相关（β = 0.16，95 % CI：0.005，0.310）。因子 3（软饮料、油炸食品和膨化食品的高摄入量）与产妇 eBMD 的变化呈反向关系（β = -0.22，95 % CI：-0.44，0.00）。母体 eBMD 的变化与 6 个月婴儿的 eBMD 呈正相关（β = 0.34，95 % CI：0.017，0.652）。总之，因子 2 可能有助于维持哺乳期妇女的 eBMD，而因子 3 则可能加剧母体 eBMD 的损失。此外，母体 eBMD 的变化与 6 个月婴儿的 eBMD 呈正相关。

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引用次数: 0

Effects of a xylitol-casein complex on insulin resistance and gut microbiota composition in high-fat-diet + streptozotocin-induced type 2 diabetes mellitus mice 木糖醇-酪蛋白复合物对高脂饮食+链脲佐菌素诱导的 2 型糖尿病小鼠胰岛素抵抗和肠道微生物群组成的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250221

This study investigated the effects of a xylitol-casein non-covalent complex (XC) on parameters related to type 2 diabetes mellitus (T2DM), in addition to related changes in gut microbiome composition and functions. High-fat-diet (HFD) + streptozotocin (STZ)-induced T2DM mice were treated with xylitol (XY), casein (CN), and XC, after which fecal samples were collected for gut microbiota composition and diversity analyses based on 16S rRNA high-throughput sequencing and multivariate statistics. XC decreased body weight and improved glucose tolerance, insulin sensitivity, pancreas impairment, blood lipid levels, and liver function in T2DM mice compared to XY- and CN-treated mice. Furthermore, XC modulated the α-diversity, β-diversity and gut microbiota composition. Based on Spearman’s correlation analysis, the relative abundances of Alistipes, Bacteroides, and Faecalibaculum were positively correlated and those of Akkermansia, Lactobacillus, Bifidobacterium, and Turicibacter were negatively correlated with the phenotypes related to the improvement of T2DM. In conclusion, we found that XC alleviated insulin resistance by restoring the gut microbiota of T2DM mice. Our results provide strong evidence for the beneficial effects of XC on T2DM and motivation for further investigation in animal models and, eventually, human trials.

本研究调查了木糖醇-酪蛋白非共价复合物（XC）对2型糖尿病（T2DM）相关参数的影响，以及肠道微生物组组成和功能的相关变化。用木糖醇（XY）、酪蛋白（CN）和XC治疗高脂饮食（HFD）+链脲佐菌素（STZ）诱导的T2DM小鼠，然后收集粪便样本，根据16S rRNA高通量测序和多元统计分析肠道微生物群的组成和多样性。与 XY 和 CN 处理的小鼠相比，XC 降低了 T2DM 小鼠的体重，改善了葡萄糖耐量、胰岛素敏感性、胰腺损伤、血脂水平和肝功能。此外，XC 还能调节α-多样性、β-多样性和肠道微生物群的组成。根据斯皮尔曼相关分析，Alistipes、Bacteroides和Faecalibaculum的相对丰度与T2DM改善相关的表型呈正相关，而Akkermansia、乳酸杆菌、双歧杆菌和Toricibacter的相对丰度与T2DM改善相关的表型呈负相关。总之，我们发现 XC 通过恢复 T2DM 小鼠的肠道微生物群缓解了胰岛素抵抗。我们的研究结果为 XC 对 T2DM 的有益作用提供了强有力的证据，也为在动物模型中进行进一步研究以及最终进行人体试验提供了动力。

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引用次数: 0

Improvement of glucose metabolism in middle-aged mice on a high-fat diet by whole-grain highland barley is related to low methionine levels 全麦高原大麦对高脂饮食中年小鼠糖代谢的改善与低蛋氨酸水平有关

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250235

Methionine restriction (MR) is an effective dietary strategy to regulate energy metabolism and alleviate oxidative stress and inflammation in the body, especially in the middle-aged and elderly population. However, the high methionine content of meat products makes this dietary strategy impossible to combine with protein supplementation and MR. Highland barley (HB), a low-methionine cereal, not only provides the body with protein but also has improved glucose metabolism and antioxidant and anti-inflammatory properties. Therefore, this study evaluated the feasibility of HB as a source of methionine-restricted dietary protein and the potential mechanisms. Middle-aged C57BL/6J mice were fed a control diet (CON), a high-fat diet (HFD), a whole-grain HB high-fat diet (HBHF), or a HBHF + methionine diet (HBHFmet) for 25 weeks. The results showed that the HBHF could keep the body weight, fasting glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR), blood lipids, inflammation, and oxidative stress of HFD mice at normal levels. Compared with the HFD groups, HBHF inhibited pancreatic cell apoptosis and improved insulin secretion while improving hepatic and skeletal muscle glucose metabolism. However, these efficacies were attenuated in HBHFmet group mice. These findings suggest that HBHF has an MR strategy.

限制蛋氨酸摄入（MR）是一种有效的膳食策略，可调节能量代谢，缓解体内氧化应激和炎症，尤其适用于中老年人群。然而，由于肉制品中蛋氨酸含量较高，因此这种饮食策略无法与补充蛋白质和MR相结合。高地大麦（HB）是一种低蛋氨酸谷物，不仅能为人体提供蛋白质，还能改善葡萄糖代谢，具有抗氧化和抗炎特性。因此，本研究评估了 HB 作为蛋氨酸限制膳食蛋白质来源的可行性及其潜在机制。研究人员给中年 C57BL/6J 小鼠喂食对照组饮食（CON）、高脂饮食（HFD）、全谷物 HB 高脂饮食（HBHF）或 HBHF + 蛋氨酸饮食（HBHFmet）25 周。结果显示，HBHF能使HFD小鼠的体重、空腹血糖、胰岛素、胰岛素抵抗平衡模型评估（HOMA-IR）、血脂、炎症和氧化应激保持在正常水平。与高脂饮食组相比，HBHF能抑制胰腺细胞凋亡，改善胰岛素分泌，同时改善肝脏和骨骼肌的葡萄糖代谢。然而，这些功效在 HBHFmet 组小鼠中有所减弱。这些研究结果表明，HBHF 具有一种 MR 策略。

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引用次数: 0

Effects of salt and rice flour concentration on microbial diversity and the quality of sour meat, a Chinese traditional meat 盐和米粉浓度对中国传统肉类--酸肉的微生物多样性和质量的影响

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250226

This study investigated the effects of salt (3 % and 6 %, m/m) and rice flour (10 % and 20 %, m/m) addition in sour meat, a traditional Chinese fermented meat. It was found that salt has greater effect than rice flour addition in spontaneous fermentation. Low-salt groups had lower pH and higher titratable total acid. In the low-salt groups, the dominant genera were Lactobacillus and Lactococcus, whereas Staphylococcus, Weissella, and Tetragenococcus were dominant in the high-salt groups. Higher total free amino acids and essential amino acids, organic acids, hexanoic acid ethyl ester and octanoic acid ethyl ester were found in the low-salt groups. The RDA analysis revealed that Lactococcus was closely related to product quality, with the S3F10 (3 % salt and 10 % rice flour) group outperforming the others in the sensory evaluation. Therefore, 3 % salt and 10 % rice flour were considered more appropriate for the production of healthy and tasty fermented sour meats.

本研究调查了在中国传统发酵肉类酸肉中添加盐（3 % 和 6 %，m/m）和米粉（10 % 和 20 %，m/m）的效果。研究发现，在自发发酵过程中，食盐的作用大于米粉。低盐组的 pH 值较低，可滴定总酸较高。低盐组的优势菌属是乳酸杆菌和乳球菌，而高盐组的优势菌属是葡萄球菌、魏氏菌和四源球菌。低盐组中游离氨基酸和必需氨基酸总量、有机酸、己酸乙酯和辛酸乙酯含量较高。RDA 分析表明，乳球菌与产品质量密切相关，S3F10（3 % 盐和 10 % 米粉）组在感官评价中优于其他组。因此，3 % 盐和 10 % 米粉被认为更适合用于生产健康美味的发酵酸肉。

引用次数: 0

Sulforaphane ameliorates non-alcoholic steatohepatitis by KLF4-mediated macrophage M2 polarization 绿藻素通过 KLF4 介导的巨噬细胞 M2 极化改善非酒精性脂肪性肝炎

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250220

Non-alcoholic fatty liver disease (NAFLD) has become a global issue and a severe threat to public health. However, to date, no approved therapeutic drugs have been developed. Dietary interventions with natural products have shown promise in preventing and treating NAFLD. Sulforaphane (SFN) is a phytocompound with antioxidant and anti-inflammatory properties, and previous research has demonstrated that SFN can ameliorate hepatic lipid accumulation and inflammation. However, the molecular mechanisms underlying these beneficial effects remain unclear. In this study, we confirmed the protective effects of SFN on excessive lipid accumulation and inflammatory injury in a high-fat, high-fructose diet-induced non-alcoholic steatohepatitis (NASH) mouse model. We found that SFN attenuates the inflammatory injury in a macrophage cell line and the liver of NASH mice, owing to the promotion of M1-type macrophage polarization toward the M2-type and the regulation of inflammatory mediators. Further analysis demonstrated that this SFN-induced macrophage M2-type polarization occurs in a Krüppel-like factor 4 (KLF4)-dependent manner. In summary, we uncovered a new mechanism of action underlying SFN activity and provide evidence that dietary intervention with SFN might be protective against NASH.

非酒精性脂肪肝（NAFLD）已成为一个全球性问题，对公众健康构成严重威胁。然而，迄今为止，尚未开发出任何获得批准的治疗药物。利用天然产品进行膳食干预已显示出预防和治疗非酒精性脂肪肝的前景。红豆杉（SFN）是一种具有抗氧化和抗炎特性的植物化合物，以往的研究表明，红豆杉可改善肝脏脂质积累和炎症。然而，这些有益作用的分子机制仍不清楚。在本研究中，我们证实了 SFN 对高脂肪、高果糖饮食诱导的非酒精性脂肪性肝炎（NASH）小鼠模型中过度脂质积累和炎症损伤的保护作用。我们发现，SFN 可减轻巨噬细胞系和 NASH 小鼠肝脏的炎症损伤，这是由于它促进了 M1 型巨噬细胞向 M2 型极化，并调节了炎症介质。进一步的分析表明，SFN诱导的巨噬细胞M2型极化是以Krüppel样因子4（KLF4）依赖的方式发生的。总之，我们揭示了 SFN 活性的新作用机制，并提供了用 SFN 进行膳食干预可预防 NASH 的证据。

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引用次数: 0