Food Science and Human Wellness最新文献_第2页

Quercetin inhibits truncated isoform of dopamine- and cAMP-regulated phosphoprotein as adjuvant treatment for trastuzumab therapy resistance in HER2-positive breast cancer 槲皮素可抑制多巴胺和 cAMP 调节磷酸蛋白的截短异构体，作为曲妥珠单抗治疗 HER2 阳性乳腺癌耐药性的辅助疗法

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250213

Trastuzumab resistance is one of the causes of poor prognosis in patients with human epidermal growth factor receptor 2 (HER2)-positive (HER2+) breast cancer (BC). The truncated isoform of dopamine- and cAMP-regulated phosphoprotein (t-DARPP) has been reported to be involved in trastuzumab therapy resistance and promoting tumor progression. To evaluate the t-DARPP expression in BC, paired tumors and surrounding normal tissues were analyzed by real-time polymerase chain reaction and confirmed higher DARPP-32 kDa family mRNA expression in HER2+ BC tumor tissues. We established 2 patient-derived xenografts (PDX) mice models to test the efficacy of trastuzumab, named model 1 (non-responder) and model 2 (responder). t-DARPP and p95-HER2 protein-protein interactions were detected in PDX tumor tissue from non-responders using Förster resonance energy transfer assays. Instead, there is no response from the responder. Furthermore, mechanistic studies using transwell and Western blot assays demonstrated that t-DARPP could upregulate epithelial-mesenchymal transition signaling proteins, enhance p95-HER2 expression and promote cell migration. We found that quercetin effectively reduced t-DARPP expression in HER2+ BC cells. In t-DARPP ShRNA-suppressed cells, quercetin synergistically enhanced trastuzumab-induced apoptotic cell death and G2/M phase arrest. In conclusion, the combination of quercetin and trastuzumab treatment by targeting t-DARPP in HER2+ BC patients has the potential as a biomarker for mitigating drug resistance.

曲妥珠单抗耐药是人类表皮生长因子受体2（HER2）阳性（HER2+）乳腺癌（BC）患者预后不良的原因之一。有报道称，多巴胺和cAMP调控磷酸蛋白（t-DARPP）的截短异构体参与了曲妥珠单抗耐药和促进肿瘤进展的过程。为了评估 t-DARPP 在 BC 中的表达，我们用实时聚合酶链反应分析了配对肿瘤和周围正常组织，结果证实在 HER2+ BC 肿瘤组织中 DARPP-32 kDa 家族 mRNA 表达较高。我们建立了两个患者衍生异种移植（PDX）小鼠模型来测试曲妥珠单抗的疗效，分别命名为模型1（无应答者）和模型2（有应答者）。相反，应答者没有任何反应。此外，利用透孔法和 Western 印迹法进行的机理研究表明，t-DARPP 可上调上皮-间质转化信号蛋白，增强 p95-HER2 的表达并促进细胞迁移。我们发现，槲皮素能有效降低 t-DARPP 在 HER2+ BC 细胞中的表达。在t-DARPP ShRNA抑制的细胞中，槲皮素能协同增强曲妥珠单抗诱导的细胞凋亡和G2/M期停滞。总之，在HER2+ BC患者中通过靶向t-DARPP联合使用槲皮素和曲妥珠单抗治疗有可能成为减轻耐药性的生物标记物。

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引用次数: 0

High β-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals 甘薯品种中的高β-谷甾醇-D-葡萄糖苷含量及其通过多种转移相关信号抗乳腺癌的潜力

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250225

β-Sitosterol-D-glucoside (β-SDG) is a phytosterol compound whose antitumor activity has been confirmed by previous studies. However, its suppression on breast cancer remains unclear. To that purpose, we isolated β-SDG from sweet potato and investigated the breast-cancer-inhibiting mechanism using proteomic analysis. The sweet potato species S6 with high β-SDG content were chosen form 36 species and β-SDG was isolated by HPLC. Afterwards, an in situ animal model of breast cancer was established, and β-SDG significantly reduced the tumor volume of MCF-7 xenograft mice. Proteomic analysis of tumor tissues revealed that 127 of these proteins were upregulated and 80 were downregulated. Gene ontology and network analysis showed that regulatory proteins were mainly associated with epithelial-mesenchymal transition (EMT), myogenesis, cholesterol homeostasis, oxidative phosphorylation and reactive oxygen pathways, while Vimentin, NDUF, VDAC1, PPP2CA and SNX9 were the most significant 5 node degree genes. Meanwhile, in vitro and in vivo results showed that the protein expression of PPP2CA and Vimentin, which are markers of EMT, were involved in breast cancer cell metastasis and could be reversed by β-SDG. This work highlights β-SDG as a bioactive compound in sweet potato and the potential therapeutic effect of β-SDG for the treatment of breast cancer by inhibiting metastasis.

β-谷甾醇-D-葡萄糖苷（β-SDG）是一种植物甾醇化合物，其抗肿瘤活性已被先前的研究证实。然而，其对乳腺癌的抑制作用仍不明确。为此，我们从甘薯中分离出了β-SDG，并利用蛋白质组分析研究了其抑制乳腺癌的机制。我们从36个甘薯品种中选出了β-SDG含量较高的甘薯品种S6，并通过高效液相色谱法分离了β-SDG。随后，建立了乳腺癌原位动物模型，β-SDG能显著减少MCF-7异种移植小鼠的肿瘤体积。肿瘤组织的蛋白质组学分析表明，其中127种蛋白质上调，80种蛋白质下调。基因本体和网络分析显示，调控蛋白主要与上皮-间质转化（EMT）、肌生成、胆固醇稳态、氧化磷酸化和活性氧通路相关，而Vimentin、NDUF、VDAC1、PPP2CA和SNX9是最重要的5个节点度基因。同时，体外和体内研究结果表明，作为EMT标志物的PPP2CA和Vimentin的蛋白表达参与了乳腺癌细胞的转移，并能被β-SDG逆转。这项研究强调了β-SDG作为甘薯中的一种生物活性化合物，以及β-SDG通过抑制转移来治疗乳腺癌的潜在疗效。

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引用次数: 0

Maillard reaction affecting immunobinding activity and digestibility of tropomyosin in Alectryonella plicatula food matrix 马氏反应影响栉水母食物基质中肌球蛋白的免疫结合活性和消化率

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250239

In recent years, the allergy rate of oysters has surged, and daily food processing methods make it hard to reduce heat resistance and digestive allergy such as tropomyosin (TM). In this study, the Maillard reaction with xylose (Xyl) significantly reduced the IgE binding capacity of Alectryonella plicatula food matrix (AFM), that reduced by (77.81 ± 2.68)%. The study found the Maillard reaction changes the structure of the AFM, in which the content of α-helix decreased by (24.64 ± 1.46)%. Structural transformation further explains why the Maillard reaction alters the immunobinding activity of AFM. In addition, the Maillard reaction reduces the digestive stability of the AFM and makes TM in the A. plicatula food matrix Maillard reaction products (AFM-MRPs) more easily digested. Based on the above research, 10 amino acids on the 7 IgE epitopes of TM were modified. This result indicates that the Maillard reaction reduces the immunobinding activity of the AFM by changing the structure and modifying the amino acids on the epitope.

近年来，牡蛎过敏率激增，日常食品加工方法难以降低耐热性和消化道过敏性，如滋养肌肽（TM）。在这项研究中，木糖（Xyl）的马氏反应显著降低了Alectryonella plicatula食物基质（AFM）的IgE结合能力，降低了（77.81 ± 2.68）%。研究发现，马氏反应改变了 AFM 的结构，其中 α-螺旋的含量减少了（24.64 ± 1.46）%。结构转变进一步解释了为什么马氏反应会改变 AFM 的免疫结合活性。此外，马氏反应降低了 AFM 的消化稳定性，使 A. plicatula 食物基质马氏反应产物（AFM-MRPs）中的 TM 更容易被消化。根据上述研究，TM 的 7 个 IgE 表位上有 10 个氨基酸被修饰。这一结果表明，马氏反应通过改变结构和修饰表位上的氨基酸，降低了 AFM 的免疫结合活性。

引用次数: 0

Adjuvant postbiotic administration improves dental caries prognosis by restoring the oral microbiota 通过恢复口腔微生物群，辅助使用后益生菌可改善龋齿预后

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250217

Conventional filling therapy fails to fundamentally reduce oral cariogenic bacteria. Thus, oral microbiota follow-up intervention after filling would be necessary for improving dental caries prognosis. We recruited 9 caries-free individuals, and 89 dental caries subjects (5 dropouts). Eighty-nine patients were randomized into three groups: caries (n = 8; no treatment), control (n = 40; filling), and postbiotics (n = 41; filling and 14-day Probio-Eco® intervention). Salivary samples were collected at 0 day (after filling) and 14 days. Our results showed that the diversity of dental caries oral microbiota was significantly increased compared with healthy subjects, and filling could restore a healthier oral microbiota partially and temporarily. Thepostbiotics intervention keeps a low alpha-diversity. Co-occurrence network analysis showed that a more stable oral microbiota structure after postbiotics intervention. Taxonomic and functional annotation of the microbiota revealed that postbiotics co-treatment significantly: increased the relative abundance of Pseudomonas and P. reactans, decreased the relative abundance of Prevotella shahii, and enriched the energy metabolism-related pathways. BugBase-predicted phenotypes inferred to an oral microbiota with decreased potential pathogenic bacteria and increased oxidative stress-tolerant bacteria after postbiotics intervention. Collectively, it suggested that postbiotics co-treatment could be a promising strategy that restores the oral microecological balance for dental caries.

传统的充填疗法无法从根本上减少口腔致龋菌。因此，有必要在补牙后对口腔微生物群进行跟踪干预，以改善龋齿的预后。我们招募了 9 名无龋患者和 89 名龋齿患者（5 人退出）。89 名患者被随机分为三组：龋齿组（n = 8；无治疗）、对照组（n = 40；补牙）和后益生菌组（n = 41；补牙和 14 天 Probio-Eco® 干预）。在 0 天（补牙后）和 14 天收集唾液样本。我们的研究结果表明，与健康受试者相比，龋齿口腔微生物群的多样性明显增加，而补牙可以部分地、暂时地恢复更健康的口腔微生物群。后益生菌干预可保持较低的α-多样性。共现网络分析显示，后生药干预后口腔微生物群结构更加稳定。微生物群的分类和功能注释显示，联合使用益生菌后处理显著增加了假单胞菌和P. reactans的相对丰度，降低了Prevotella shahii的相对丰度，并丰富了能量代谢相关通路。根据 BugBase 预测的表型推断，口腔微生物群中潜在的致病菌减少了，耐氧化应激细菌增加了。总之，这表明益生菌后联合治疗可能是恢复龋齿口腔微生态平衡的一种有前途的策略。

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引用次数: 0

Overview on signal transduction cascades regulation roles of garlic and its bioactive constituents 大蒜及其生物活性成分的信号转导级联调控作用概述

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250196

High-throughput technologies in combination with modern exciting advancements in mass spectrometry-based proteomics and data analysis pipelines have empowered comprehensive characterization of disease phenotypes and their mechanistic regulation by dietary agents and bioactive molecules at unprecedented dimensionality and resolution. Extra-ordinary breakthroughs in the field of nutrigenomics have leveraged our understanding altogether to a new level of maturity. Interdisciplinary researchers have extensively analyzed health promoting and pharmacologically significant properties of garlic (Allium sativum). Importantly, garlic and its biologically active chemicals targeted oncogenic signaling cascades. In this mini-review we have attempted to summarize how garlic and its bioactive constituents regulated signal transduction cascades in cell culture studies and tumor-bearing mice.

高通量技术与基于质谱的蛋白质组学和数据分析管道方面令人振奋的现代进步相结合，以前所未有的维度和分辨率对疾病表型及其受膳食因子和生物活性分子调控的机理进行了全面描述。营养基因组学领域的非凡突破将我们的认识提升到了一个新的成熟水平。跨学科研究人员广泛分析了大蒜（Allium sativum）促进健康和具有药理学意义的特性。重要的是，大蒜及其生物活性化学物质针对的是致癌信号级联。在这篇小型综述中，我们试图总结大蒜及其生物活性成分如何在细胞培养研究和肿瘤小鼠中调节信号转导级联。

引用次数: 0

Molecular mechanisms of aflatoxin neurotoxicity and potential neuroprotective agents 黄曲霉毒素神经毒性的分子机制和潜在的神经保护剂

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250201

Aflatoxins (AFTs) represent one of the most notorious classes of deadly mycotoxins produced by certain fungi that are found on agricultural crops. Aflatoxins are highly toxic to mammals and are known to cause a series of detrimental effects, including neuro-, hepato-, nephron-, and immuno-toxicity. In this original review we summarize the mechanisms of aflatoxin-induced neurotoxicity and the clinical potential of novel neuroprotective agents. Aflatoxin B1 (AFB1) is the most toxic congener among the 21 identified AFTs. Recent studies have shown that food borne exposure to AFB1 and/or its metabolites often leads to fatal neurotoxicity in animals and humans. Animal studies indicated that AFB1 exposure could induce abnormal behavioral changes, including anxiety, lethargy disorders, depression-like behavior, cognitive, learning and memory defects, and decreased feeding behavior. Mechanistically, AFB1 exposure has been associated with lipid peroxidation, ablation of non-enzymatic and enzymatic antioxidant defense systems and decreased neurotransmitter levels. AFB1 exposure has also been shown to induce DNA damage, apoptosis, pyroptosis, and mitochondrial dysfunction in the brain tissue. Several signaling pathways, including gasdermin D, toll like receptor 2 (TLR2), TLR4, Akt, NF-κB, ERK/MAPK, protein kinase C (PKC), and mitochondrial apoptotic pathways have been shown to participate in AFB1-induced neuronal or astrocyte cell death. Targeting these pathways by small molecules (e.g., quercetin, curcumin, and gallic acid, and dimethyl fumarate), Chinese herbal extracts (e.g., Artichoke leaf extract, Chelidonium majus ethanolic extract, pumpkin extract, and Crocus sativus L. tea), and probiotic supplements could effectively improve AFB1-induced neurobehavioral abnormalities and neurotoxicity. To date, the precise molecular mechanisms of AFB1-induced neurotoxicity and potential neuroprotective agents remain unclear. In the present review, the clinical manifestations, molecular mechanisms, and potential neuroprotective agents of AFB1-induced neurotoxicity are summarized in the broadest overview. It is most hopeful that this broad reaching review provides valuable insights and stimulates broader discussion to develop the effective neuroprotective agents against aflatoxins.

黄曲霉毒素（AFTs）是最臭名昭著的一类致命霉菌毒素，由农作物上的某些真菌产生。黄曲霉毒素对哺乳动物有剧毒，已知会造成一系列有害影响，包括神经、肝、肾和免疫毒性。在这篇原创性综述中，我们总结了黄曲霉毒素诱发神经毒性的机制以及新型神经保护剂的临床潜力。黄曲霉毒素 B1（AFB1）是已发现的 21 种黄曲霉毒素中毒性最强的同系物。最近的研究表明，动物和人类通过食物摄入黄曲霉毒素 B1 和/或其代谢物往往会导致致命的神经中毒。动物研究表明，摄入 AFB1 可诱发异常行为变化，包括焦虑、嗜睡障碍、抑郁样行为、认知、学习和记忆缺陷以及摄食行为减少。从机理上讲，暴露于 AFB1 与脂质过氧化、非酶和酶抗氧化防御系统的消减以及神经递质水平的下降有关。研究还表明，暴露于 AFB1 会诱发脑组织的 DNA 损伤、细胞凋亡、热凋亡和线粒体功能障碍。一些信号通路，包括 gasdermin D、toll like receptor 2 (TLR2)、TLR4、Akt、NF-κB、ERK/MAPK、蛋白激酶 C (PKC) 和线粒体凋亡通路已被证明参与了 AFB1 诱导的神经元或星形胶质细胞死亡。通过小分子药物（如槲皮素、姜黄素、没食子酸和富马酸二甲酯）、中草药提取物（如朝鲜蓟叶提取物、车前子乙醇提取物、南瓜提取物和鳄梨茶）和益生菌补充剂来靶向这些通路，可有效改善 AFB1 诱导的神经行为异常和神经毒性。迄今为止，AFB1诱导神经毒性的确切分子机制和潜在的神经保护剂仍不清楚。本综述对 AFB1 诱导的神经毒性的临床表现、分子机制和潜在的神经保护剂进行了最广泛的概述。希望这篇内容广泛的综述能提供有价值的见解，并引发更广泛的讨论，从而开发出有效的黄曲霉毒素神经保护剂。

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引用次数: 0

Dioscin from Polygonatum sibiricum induces apoptosis and autophagy in Ishikawa human endometrial cancer cell and in vivo 何首乌中的薯蓣皂苷可诱导石川人子宫内膜癌细胞和体内细胞凋亡和自噬

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250209

With an aim to comprehend the precise regulatory mechanism of dioscin against endometrial carcinoma (EC), we firstly extracted the components from Polygonatum sibiricum followed by identification and structural characterization. The anti-EC activity of dioscin was initially determined based on the inhibition of Ishikawa cell proliferation and tumor growth. The high-throughput sequencing data indicated that dioscin not only promoted apoptosis, including decrease of poly ADP-ribose polymerase (PARP) and B-cell lymphoma-2 (Bcl-2) and increase of c-PARP and Bcl-2-associcated agonist of cell death (Bad), but also induced autophagy, including increase of autophagic lysosomes and LC3II/LC3Ⅰ ratio. Mechanistic exploration suggested that dioscin induced autophagy and apoptosis through inhibition of PI3K/AKT/mTOR signaling pathway. Besides, the dioscin-regulated p53 pathway was mainly involved in autophagy induction. Furthermore, inhibition of Ishikawa cell autophagy was linked to dioscin-induced apoptosis. Our data suggest the immense potential of dioscin for the development of functional food for EC and related medical application.

为了理解地奥司钦对子宫内膜癌（EC）的精确调控机制，我们首先从西伯利亚何首乌中提取了成分，然后进行了鉴定和结构表征。根据对石川细胞增殖和肿瘤生长的抑制作用，初步确定了薯蓣皂苷的抗子宫内膜癌活性。高通量测序数据表明，薯蓣皂苷不仅能促进细胞凋亡，包括减少多聚 ADP 核糖聚合酶（PARP）和 B 细胞淋巴瘤-2（Bcl-2），增加 c-PARP 和 Bcl-2 细胞死亡激动剂（Bad），还能诱导自噬，包括增加自噬溶酶体和 LC3II/LC3Ⅰ 比值。机理研究表明，地奥辛通过抑制PI3K/AKT/mTOR信号通路诱导自噬和细胞凋亡。此外，地奥司钦调控的p53通路主要参与自噬诱导。此外，石川细胞自噬的抑制与 dioscin 诱导的细胞凋亡有关。我们的数据表明，薯蓣皂苷在开发欧共体功能食品和相关医疗应用方面具有巨大潜力。

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引用次数: 0

Lactobacillus from fermented bamboo shoots prevents inflammation in DSS-induced colitis mice via modulating gut microbiome and serum metabolites 发酵竹笋中的乳酸菌通过调节肠道微生物组和血清代谢物预防 DSS 诱导的结肠炎小鼠的炎症

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250229

Fermented bamboo shoots (FBS) is a region-specific food widely consumed in Southwestern China, with Lactobacillus as the predominant fermenting bacteria. However, the probiotic potential of Lactobacillus derived from FBS reminds largely unexplored, especially for diseases with a low prevalence in areas consuming FBS, namely, inflammatory bowel disease. In this study, Lactiplantibacillus pentosus YQ001 and Lentilactobacillus senioris YQ005 were screening by in vitro probiotic tests to further investigate the probiotic-like bioactivity in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mouse. They exhibited more positive probiotic effects than Lactobacillus rhamnosus GG in preventing intestinal inflammatory response. The results revealed that both strains improved the abundance of deficient intestinal microbiota in UC mice, including Muribaculaceae and Akkermansia. In the serum metabolome, they modulated the DSS-disturbed levels of metabolites, with significant increment of cinnamic acid. Meanwhile, they reduced the expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) inflammatory factors and increased zonula occludens-1 (ZO-1), Occludin, and cathelicidin-related antimicrobial peptide (CRAMP) in the colon. Consequently, these results demonstrated that Lactobacillus spp. isolates derived from FBS showed promising probiotic activity based on the gut microbiome homeostasis modulation, anti-inflammation and intestinal barrier protection in UC mice.

发酵竹笋（FBS）是中国西南地区广泛食用的一种地方特色食品，其主要发酵菌是乳酸杆菌。然而，从发酵竹笋中提取的乳酸杆菌的益生潜力尚未得到充分开发，尤其是对食用发酵竹笋的地区发病率较低的疾病（即炎症性肠病）的益生潜力。本研究通过体外益生菌试验筛选了戊糖乳杆菌（Lactiplantibacillus pentosus YQ001）和高级扁豆乳杆菌（Lentilactobacillus senioris YQ005），以进一步研究它们在葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）小鼠中的益生菌样生物活性。与鼠李糖乳杆菌 GG 相比，它们在预防肠道炎症反应方面表现出更积极的益生作用。研究结果表明，这两种菌株都能改善 UC 小鼠肠道微生物区系的丰度，包括 Muribaculaceae 和 Akkermansia。在血清代谢组中，这两种菌株调节了被DSS干扰的代谢物水平，其中肉桂酸显著增加。同时，它们降低了结肠中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）等炎症因子的表达水平，并增加了结肠中Zonula occludens-1（ZO-1）、Occludin和cathelicidin相关抗菌肽（CRAMP）的表达水平。因此，这些结果表明，基于对 UC 小鼠肠道微生物组平衡的调节、抗炎和肠道屏障保护，从 FBS 中分离的乳酸杆菌具有良好的益生菌活性。

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引用次数: 0

Longitudinal investigation of mineral composition in human milk and its correlation with infant anthropometric outcomes among Tibetan mother-infant dyads during the first 6 months postpartum 产后 6 个月藏族母婴二人组母乳中矿物质成分及其与婴儿人体测量结果相关性的纵向调查

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250231

Nutrients in human milk, including minerals, relate growth and development of breast-fed infants. Tibetan mother-infant dyads possess unique characteristics on early nutrition due to their featured long-lasting life-style. This study longitudinally investigated the relationship between the mineral composition in human milk and the Z-scores of infants among Tibetan mother-infant dyads during their first 6 months postpartum through a prospective cohort study. The results show that the minerals of Na, Mg, K, Ca, Cu, Zn, and Se were of higher levels in colostrum than other lactation stages. Several minerals were below the recommended values for infants according to Chinese dietary guidelines. Besides, a large proportion of infant Z-scores were below −2 as lactation period continued. Multivariate statistical analysis revealed that classifications and correlations in varying degrees were observed between minerals in human milk and infant Z-scores. These findings will be advantageous for research upon Chinese early nutrition and progress of tailor-made infant formula.

母乳中的营养物质（包括矿物质）与母乳喂养婴儿的生长发育息息相关。藏族母婴由于其独特的长期生活方式，在早期营养方面具有独特的特点。本研究通过前瞻性队列研究，纵向调查了藏族母婴产后 6 个月内母乳中矿物质成分与婴儿 Z 评分之间的关系。结果显示，初乳中 Na、Mg、K、Ca、Cu、Zn 和 Se 等矿物质的含量高于其他哺乳阶段。有几种矿物质低于中国膳食指南对婴儿的推荐值。此外，随着哺乳期的延长，大部分婴儿的 Z 值低于-2。多变量统计分析显示，母乳中的矿物质与婴儿 Z 值之间存在不同程度的分类和相关性。这些研究结果将有助于中国早期营养的研究和量身定制婴儿配方奶粉的进展。

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引用次数: 0

DIA-based quantitative proteomic analysis on porcine meat quality at different chilling rates 基于 DIA 的不同冷冻速率下猪肉质量的定量蛋白质组分析

IF 5.6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Science and Human Wellness

Pub Date : 2024-09-01 DOI: 10.26599/FSHW.2022.9250206

The objective of this study was to evaluate the effects of chilling rate on porcine meat quality from the perspective of proteome using data independent acquisition (DIA)-based quantitative proteomic strategy. M. longissimus thoracis et lumborum (n = 9) was assigned randomly to the control group (3.72 °C/h), very fast chilling-I group (VFC-I, 9.31 °C/h) and VFC-II group (14.43 °C/h). The DIA was used to analyze the difference in proteins under different chilling rates. Results showed that tenderness was improved significantly in meat at the chilling rate of 14.43 °C/h. Seventy-nine differential abundant proteins (fold change > 1.5, P < 0.05), including 46 up-regulated and 33 down-regulated proteins, were identified and mainly involved in carbon metabolism, pyruvate metabolism and proteasome pathways. These pathways indicated that VFC delayed cell metabolism and glycolysis by down-regulating the expression of metabolic enzymes. The tenderness was improved by up-regulating the expression of proteasome and m-calpain.

本研究旨在采用基于数据独立采集（DIA）的定量蛋白质组学策略，从蛋白质组的角度评估冷冻速度对猪肉品质的影响。将长胸肌和腰肌（n = 9）随机分配到对照组（3.72 °C/h）、快速冷却-I 组（VFC-I，9.31 °C/h）和 VFC-II 组（14.43 °C/h）。使用 DIA 分析了不同冷却速度下蛋白质的差异。结果表明，冷冻速率为 14.43 °C/h 时，肉的嫩度明显提高。共鉴定出79个差异丰度蛋白（折合变化率为1.5，P为0.05），包括46个上调蛋白和33个下调蛋白，主要涉及碳代谢、丙酮酸代谢和蛋白酶体途径。这些途径表明，VFC 通过下调代谢酶的表达，延迟了细胞代谢和糖酵解。通过上调蛋白酶体和 m-calpain 的表达，嫩度得到改善。

引用次数: 0