Pub Date : 2025-09-01DOI: 10.26599/fshw.2025.9250788
Sijuan Wang, Jingwen Xu, Kaiqiang Lv, Guangwen Luo, Ning Guo, Yijie Li, Jinyuan Sun, Guoliang Li
{"title":"Effects of carbon quantum dots on the growth of \u0000 <em>Lactobacillus plantarum</em> ATCC 8014 due to oxidative stress and cell integrity destruction","authors":"Sijuan Wang, Jingwen Xu, Kaiqiang Lv, Guangwen Luo, Ning Guo, Yijie Li, Jinyuan Sun, Guoliang Li","doi":"10.26599/fshw.2025.9250788","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250788","url":null,"abstract":"","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-01DOI: 10.26599/fshw.2025.9250695
Xiaoting Yu, Yannan Chen, Wentao Su, Haitao Wang, Jia-lin Miao, Y. Bai, Xunyu Song, Mingqian Tan
Circadian rhythm plays a crucial role in maintaining intestinal homeostasis, and conversely, the remodeling of gut microbiota can help regulate circadian rhythm. Although traditional therapeutic strategies involving oral small-molecule drugs can modulate the intestinal health in circadian rhythm disruption (CRD), they are limited by restricted efficacy, dependence, and side effects. Herein, astaxanthin (AXT) and Spirulina platensis (SP) were used to prepare an innovative complex (AXT@SP) by a simple one-step synthesis method to alleviate the excessive intestinal reactive oxygen species (ROS) in CRD. The AXT@SP successfully achieved efficient loading of AXT with the drug loading efficiency of 34.56% and extended the retention time of AXT in the intestine for more than 12 h. As expected, AXT@SP reduced the intestinal ROS generation by 40.7%, restored the circadian oscillation of antioxidant-related indicators, protected the intestinal immune barrier, and reshaped the gut microbiota composition in the intestine of CRD mice. Importantly, AXT@SP restored the circadian peak of γ-aminobutyric acid during the physiological sleeping phase and regulated the phase advance of cortisol at ZT12. Our results suggested that AXT@SP could be exploited as a promising strategy for regulating CRD-related intestinal homeostasis via the gut-brain axis.
{"title":"Alleviation of excessive intestinal reactive oxygen species induced by circadian rhythm disruption through the Astaxanthin@ <em>Spirulina platensis</em> complex","authors":"Xiaoting Yu, Yannan Chen, Wentao Su, Haitao Wang, Jia-lin Miao, Y. Bai, Xunyu Song, Mingqian Tan","doi":"10.26599/fshw.2025.9250695","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250695","url":null,"abstract":"Circadian rhythm plays a crucial role in maintaining intestinal homeostasis, and conversely, the remodeling of gut microbiota can help regulate circadian rhythm. Although traditional therapeutic strategies involving oral small-molecule drugs can modulate the intestinal health in circadian rhythm disruption (CRD), they are limited by restricted efficacy, dependence, and side effects. Herein, astaxanthin (AXT) and <em>Spirulina platensis </em>(SP) were used to prepare an innovative complex (AXT@SP) by a simple one-step synthesis method to alleviate the excessive intestinal reactive oxygen species (ROS) in CRD. The AXT@SP successfully achieved efficient loading of AXT with the drug loading efficiency of 34.56% and extended the retention time of AXT in the intestine for more than 12 h. As expected, AXT@SP reduced the intestinal ROS generation by 40.7%, restored the circadian oscillation of antioxidant-related indicators, protected the intestinal immune barrier, and reshaped the gut microbiota composition in the intestine of CRD mice. Importantly, AXT@SP restored the circadian peak of γ-aminobutyric acid during the physiological sleeping phase and regulated the phase advance of cortisol at ZT12. Our results suggested that AXT@SP could be exploited as a promising strategy for regulating CRD-related intestinal homeostasis <em>via</em> the gut-brain axis.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://file.sciopen.com/sciopen_public/1963419381821603842.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Modulation of complement C3/C3aR pathway by paeoniflorin, a major constituent of Paeoniae Radix Alba, ameliorates vascular cognitive impairment under the theory of medicinal food homology","authors":"Jin Wang, Jingwen Wu, Minjie Sun, Yuejie Zhang, Ying Xu, Xiang Li, Boran Zhu, Xin Gu","doi":"10.26599/fshw.2025.9250690","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250690","url":null,"abstract":"","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01DOI: 10.26599/fshw.2025.9250667
Xin Mao, Yifang Hong, Xinru Li, Haoxian An, Shuang Zhou, Huihui Liu, Bin Qiu, Yanli You
Mequindox (MEQ), a member of Quinoxaline 1, 4–di–N–oxides (QdNOs), has been applied in Scophthalmus maximus farming industry. Due to the severe toxic effects of QdNOs, the residue of MEQ and its metabolites in Scophthalmus maximus are food safety risk factors. In this work, metabolism of MEQ in Scophthalmus maximus in vivo was investigated. Eight metabolites were identified to be formed via hydrogenation reduction, desoxygenation, deacetylation, methyl mono–hydroxylation, and their combination reactions. Different from land farm animal with desoxygenation as major metabolic reaction, carbonyl reduction is the major metabolic reaction in Scophthalmus maximus. In addition, M8 (3–methylol–BDMEQ) was first discovered in aquatic organisms with methyl mono–hydroxylation as a novel metabolic reaction, and M7 (Deacetyl–4–DMEQ) was the first metabolite to be detected in an organism undergoing both N→O deoxygenation and deacetylation reactions. This work revealed metabolic reactions of MEQ in Scophthalmus maximus and provided the process to study this metabolism in other marine animals.
{"title":"Identification of Eight <em>in vivo</em> Metabolites of Mequindox in <em>Scophthalmus maximus</em> by using UHPLC-MS/MS","authors":"Xin Mao, Yifang Hong, Xinru Li, Haoxian An, Shuang Zhou, Huihui Liu, Bin Qiu, Yanli You","doi":"10.26599/fshw.2025.9250667","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250667","url":null,"abstract":"Mequindox (MEQ), a member of Quinoxaline 1, 4–di–N–oxides (QdNOs), has been applied in<em> Scophthalmus maximus</em> farming industry. Due to the severe toxic effects of QdNOs, the residue of MEQ and its metabolites in <em>Scophthalmus maximus</em> are food safety risk factors. In this work, metabolism of MEQ in <em>Scophthalmus maximus in vivo</em> was investigated. Eight metabolites were identified to be formed via hydrogenation reduction, desoxygenation, deacetylation, methyl mono–hydroxylation, and their combination reactions. Different from land farm animal with desoxygenation as major metabolic reaction, carbonyl reduction is the major metabolic reaction in <em>Scophthalmus maximus</em>. In addition, M8 (3–methylol–BDMEQ) was first discovered in aquatic organisms with methyl mono–hydroxylation as a novel metabolic reaction, and M7 (Deacetyl–4–DMEQ) was the first metabolite to be detected in an organism undergoing both N→O deoxygenation and deacetylation reactions. This work revealed metabolic reactions of MEQ in Scophthalmus maximus and provided the process to study this metabolism in other marine animals.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sciopen.com/article_pdf/1943481174946574338.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-01DOI: 10.26599/fshw.2025.9250665
Hao Jiang, Lu Dai, Yin Chen, Xingbo Shi, Yan Lv
In food processing, avoiding contamination of pathogens while not affecting the nutritional composition of foods is crucial. Herein, a Halbach ring mediated continuous flow non-thermal antibacterial (HCFNA) platform for the eradication of pathogenic bacteria is developed by employing magnetic nanoparticle@Ag Prussian blue analogues (MNP@AgPBAs). MNP@AgPBAs with excellent non-thermal antibacterial property and magnetic responsive performance are synthesized through in-situ growth method and ion exchange process. Particularly, the Halbach ring can enrich and align magnetic materials to form a magnetic net, enhancing the contact between materials and bacteria. Moreover, the Halbach ring can be integrated with a microinjection pump to achieve continuous flow antibacterial operation. The safety of MNP@AgPBAs is confirmed by in vitro cytotoxicity tests, indicating the admirable biocompatibility of MNP@AgPBAs and their promising application in the food industry. The HCFNA platform acquires sterilization efficiencies of 99.91% and 98.19% against Gram-negative bacteria （Escherichia coli, E. coli) and Gram-positive bacteria （Staphylococcus aureus, S. aureus), respectively. Besides, the HCFNA platform exhibits robust antibacterial activity in real food samples such as milk, tea, and drinking water, showcasing well-adapted properties to diverse liquid food matrices. Compared to heat treatment, the HCFNA platform exerts a lesser impact on actual samples, which have been proven by nutrient quality analysis. Hence, the HCFNA platform possesses the capability to realize continuous flow antibacterial operation and reduce nutrient loss in food production and processing, making it a promising candidate for antibacterial platforms in the food industry.
{"title":"A Continuous Flow Non-Thermal Antibacterial Platform for Foodborne Pathogens Based on Halbach Ring Mediated Magnetic Net","authors":"Hao Jiang, Lu Dai, Yin Chen, Xingbo Shi, Yan Lv","doi":"10.26599/fshw.2025.9250665","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250665","url":null,"abstract":"In food processing, avoiding contamination of pathogens while not affecting the nutritional composition of foods is crucial. Herein, a Halbach ring mediated continuous flow non-thermal antibacterial (HCFNA) platform for the eradication of pathogenic bacteria is developed by employing magnetic nanoparticle@Ag Prussian blue analogues (MNP@AgPBAs). MNP@AgPBAs with excellent non-thermal antibacterial property and magnetic responsive performance are synthesized through in-situ growth method and ion exchange process. Particularly, the Halbach ring can enrich and align magnetic materials to form a magnetic net, enhancing the contact between materials and bacteria. Moreover, the Halbach ring can be integrated with a microinjection pump to achieve continuous flow antibacterial operation. The safety of MNP@AgPBAs is confirmed by in vitro cytotoxicity tests, indicating the admirable biocompatibility of MNP@AgPBAs and their promising application in the food industry. The HCFNA platform acquires sterilization efficiencies of 99.91% and 98.19% against Gram-negative bacteria （<em>Escherichia coli</em>, <em>E. coli</em>) and Gram-positive bacteria （<em>Staphylococcus aureus</em>, <em>S. aureus</em>), respectively. Besides, the HCFNA platform exhibits robust antibacterial activity in real food samples such as milk, tea, and drinking water, showcasing well-adapted properties to diverse liquid food matrices. Compared to heat treatment, the HCFNA platform exerts a lesser impact on actual samples, which have been proven by nutrient quality analysis. Hence, the HCFNA platform possesses the capability to realize continuous flow antibacterial operation and reduce nutrient loss in food production and processing, making it a promising candidate for antibacterial platforms in the food industry. ","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sciopen.com/article_pdf/1943472251824861186.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-06-01DOI: 10.26599/fshw.2025.9250558
Xiaolong Chen, Tong Liu, Jiaojiao Han, Xiao Li, Yufei Wu, Lulu Wang, Yueyang Zhong, Yafei Ji, Kai Zhou, Xiurong Su, Chi-Tang Ho, Chenyang Lu
Artemisia argyi (A. argyi) is a Chinese herbal medicine with reported anti-inflammatory effects. In this study, the A. argyi was extracted with water and ethanol, and the concentrations of 35 flavonoids in A. argyi water extract (WE) and ethanol extract (EE) were measured via targeted metabolomics. The antioxidant and anti-inflammatory activities of both WE and EE were firstly explored in vitro via chemical assays and cellular experiment, respectively. Both WE and EE showed significant DPPH, ABTS, OH-, and O2-radical scavenging ability in a dose-dependent manner, and reduced the levels of IL-1β, TNF-α and IL-22 in LPS induced RAW264.7 cell model. In addition, the in vivo anti-colitis activity of both extracts was performed in dextran sulfate sodium (DSS)-induced colitis mouse, and the mechanisms were elucidated by 16S rDNA sequencing and targeted metabolomics. We found that both WE and EE relieved colitis in mice, characterized by decreased disease activity index, increased colon length, improved pathological changes in colon tissue, while EE showed better anti-colitis activity. In addition, both 16S rDNA sequencing and targeted bile acids metabolomics indicated EE modulated gut microbiota and specifically increased the abundance of lithocholic acid, which might contribute to intestinal barrier function improvement via up-regulating the expression of colonic FXR. In summary, this study identified the anti-colitis mechanism of A. argyi ethanol extract by modulating gut microbiota, facilitating the production of lithocholic acid, activating FXR and improving intestinal barrier function.
{"title":"<em>Artemisia argyi</em>extracts alleviated colitis in mice via modulating gut microbiota and bile acid metabolism","authors":"Xiaolong Chen, Tong Liu, Jiaojiao Han, Xiao Li, Yufei Wu, Lulu Wang, Yueyang Zhong, Yafei Ji, Kai Zhou, Xiurong Su, Chi-Tang Ho, Chenyang Lu","doi":"10.26599/fshw.2025.9250558","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250558","url":null,"abstract":"<em>Artemisia argyi</em> (<em>A. argyi</em>) is a Chinese herbal medicine with reported anti-inflammatory effects. In this study, the <em>A. argyi</em> was extracted with water and ethanol, and the concentrations of 35 flavonoids in <em>A. argyi</em> water extract (WE) and ethanol extract (EE) were measured via targeted metabolomics. The antioxidant and anti-inflammatory activities of both WE and EE were firstly explored <em>in vitro</em> via chemical assays and cellular experiment, respectively. Both WE and EE showed significant DPPH, ABTS, OH<sup>-</sup>, and O<sub>2</sub><sup>-</sup><sub> </sub>radical scavenging ability in a dose-dependent manner, and reduced the levels of IL-1β, TNF-α and IL-22 in LPS induced RAW264.7 cell model. In addition, the <em>in vivo </em>anti-colitis activity of both extracts was performed in dextran sulfate sodium (DSS)-induced colitis mouse, and the mechanisms were elucidated by 16S rDNA sequencing and targeted metabolomics. We found that both WE and EE relieved colitis in mice, characterized by decreased disease activity index, increased colon length, improved pathological changes in colon tissue, while EE showed better anti-colitis activity. In addition, both 16S rDNA sequencing and targeted bile acids metabolomics indicated EE modulated gut microbiota and specifically increased the abundance of lithocholic acid, which might contribute to intestinal barrier function improvement via up-regulating the expression of colonic FXR. In summary, this study identified the anti-colitis mechanism of <em>A. argyi</em> ethanol extract by modulating gut microbiota, facilitating the production of lithocholic acid, activating FXR and improving intestinal barrier function.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sciopen.com/article_pdf/1935582557984354305.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147330507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A strict and lifelong dietary intake control of low-Phe foods is an important therapy method for the patients with Phe hydroxylase deficiency. As an excellent protein source, casein has been used to prepare low-Phe polypeptides for the potential development of low-Phe foods. However, casein has not been explored as the protein source to prepare low-Phe polypeptides. This work aimed to develop casein polypeptides (CPs) and low-Phe CPs (LCPs) from milk protein casein using a combination method of two-step (alkaline protease and then flavourzyme) enzymatic hydrolysis with activated carbon adsorption. The preparation of the LCPs was dependent on several key parameters such as enzyme/casein mass ratios, enzymatic hydrolysis time, adsorption pH, adsorption temperature, activated carbon addition amounts, and adsorption time. The LCPs showed similar attenuated total reflection Fourier-transform infrared spectra and secondary structure percentages to the CP. The LCPs had lower MWs (1121 – 4726 Da vs. 10 – 38 kDa) and Phe contents (3.31% – 1.35% vs.4.56%) compared with casein. The LCPs had better fat-binding ability (3.15 – 1.21 g/g), lower emulsifying activity ability (0.25 – 0.21 vs. 0.98 m2/g), higher emulsifying stability ability (16.7 – 24.44 vs. 11.78 min), higher 2,2-diphenyl-1-picrylhydrazyl free radical scavenging capacity (38.62% – 41.06% vs. 36.59%), higher reducing capacity (0.011 – 0.013 vs. 0.007), and lower Fe2+-chelating ability (19.61% – 17.65% vs. 41.18%) than CPs. They suggested aromatic AA-related CP fragments had different properties to other CP fragments. All the results confirmed low-Phe polypeptides could be prepared from casein via the combination method of two-step (alkaline protease and then flavourzyme) enzymatic hydrolysis with activated carbon adsorption. The work provided a useful method to prepare low-Phe polypeptides from milk protein casein for the development of low-Phe foods. It also provided useful information to understand the structure and function of hydrolyzed polypeptides and amino acid-deficiency polypeptides.
{"title":"Preparation and functional properties of low-phenylalanine casein polypeptides","authors":"Qiqi Bian, Lijia Chen, Wenjie Zhang, Zhengquan Wang, Xichang Wang, Jian Zhong","doi":"10.26599/fshw.2025.9250644","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250644","url":null,"abstract":"A strict and lifelong dietary intake control of low-Phe foods is an important therapy method for the patients with Phe hydroxylase deficiency. As an excellent protein source, casein has been used to prepare low-Phe polypeptides for the potential development of low-Phe foods. However, casein has not been explored as the protein source to prepare low-Phe polypeptides. This work aimed to develop casein polypeptides (CPs) and low-Phe CPs (LCPs) from milk protein casein using a combination method of two-step (alkaline protease and then flavourzyme) enzymatic hydrolysis with activated carbon adsorption. The preparation of the LCPs was dependent on several key parameters such as enzyme/casein mass ratios, enzymatic hydrolysis time, adsorption pH, adsorption temperature, activated carbon addition amounts, and adsorption time. The LCPs showed similar attenuated total reflection Fourier-transform infrared spectra and secondary structure percentages to the CP. The LCPs had lower MWs (1121 – 4726 Da vs. 10 – 38 kDa) and Phe contents (3.31% – 1.35% vs.4.56%) compared with casein. The LCPs had better fat-binding ability (3.15 – 1.21 g/g), lower emulsifying activity ability (0.25 – 0.21 vs. 0.98 m<sup>2</sup>/g), higher emulsifying stability ability (16.7 – 24.44 vs. 11.78 min), higher 2,2-diphenyl-1-picrylhydrazyl free radical scavenging capacity (38.62% – 41.06% vs. 36.59%), higher reducing capacity (0.011 – 0.013 vs. 0.007), and lower Fe<sup>2+</sup>-chelating ability (19.61% – 17.65% vs. 41.18%) than CPs. They suggested aromatic AA-related CP fragments had different properties to other CP fragments. All the results confirmed low-Phe polypeptides could be prepared from casein via the combination method of two-step (alkaline protease and then flavourzyme) enzymatic hydrolysis with activated carbon adsorption. The work provided a useful method to prepare low-Phe polypeptides from milk protein casein for the development of low-Phe foods. It also provided useful information to understand the structure and function of hydrolyzed polypeptides and amino acid-deficiency polypeptides.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sciopen.com/article_pdf/1932713128153956354.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Irritable bowel syndrome (IBS), a functional gastrointestinal disorder characterized by impaired digestive function, predominantly presents as the diarrhea-predominant subtype (IBS-D). Current therapeutic strategies for IBS-D primarily aim to restore intestinal barrier integrity and regulate secretory homeostasis. However, progress in therapeutic development has been limited by an incomplete understanding of the microbial sensing mechanisms that govern intestinal barrier dynamics. This study investigates the therapeutic efficacy of paeoniflorin (PF) in IBS-D treatment and elucidates its underlying mechanisms. Through comprehensive preclinical evaluation, PF administration significantly ameliorated diarrheal symptoms and psychiatric comorbidities in IBS-D rats, concomitant with structural restoration of intestinal villi and functional recovery of secretory regulation. Mechanistically, PF exerted its therapeutic effects through gut microbiota-dependent attenuation of sIgA hypersecretion. Notably, fecal microbiota transplantation and depletion experiments revealed that PF-mediated intestinal barrier repair and secretory normalization were operated through s_Lachnospiraceae_bacterium (L. bacterium) modulation. Functional characterization demonstrated that L. bacterium alleviated IBS-D pathophysiology by enhancing tight junction assembly and facilitating mucin maturation. Furthermore, mechanistic analysis identified FoxO3a signaling as a pivotal mediator of the intestinal protection conferred by L. bacterium. Collectively, this study not only establishes PF as a promising therapeutic candidate but also uncovers novel microbial-epithelial crosstalk mechanisms, highlighting the translational potential of probiotic-based interventions and bioactive compounds for intestinal disorders. These findings provide clinically relevant insights for the development of targeted therapeutics for IBS-D.
{"title":"Multi-omics reveal mechanisms of <em>L. bacterium</em> mediated intestinal protective effect of paeoniflorin in diarrhea-predominant irritable bowel syndrome","authors":"Yuanyuan Wei, Chao Han, Yimeng Fan, Sijuan Huang, Zhuo Chen, Jianyu Lv, Zhihui Hao","doi":"10.26599/fshw.2025.9250555","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250555","url":null,"abstract":"Irritable bowel syndrome (IBS), a functional gastrointestinal disorder characterized by impaired digestive function, predominantly presents as the diarrhea-predominant subtype (IBS-D). Current therapeutic strategies for IBS-D primarily aim to restore intestinal barrier integrity and regulate secretory homeostasis. However, progress in therapeutic development has been limited by an incomplete understanding of the microbial sensing mechanisms that govern intestinal barrier dynamics. This study investigates the therapeutic efficacy of paeoniflorin (PF) in IBS-D treatment and elucidates its underlying mechanisms. Through comprehensive preclinical evaluation, PF administration significantly ameliorated diarrheal symptoms and psychiatric comorbidities in IBS-D rats, concomitant with structural restoration of intestinal villi and functional recovery of secretory regulation. Mechanistically, PF exerted its therapeutic effects through gut microbiota-dependent attenuation of sIgA hypersecretion. Notably, fecal microbiota transplantation and depletion experiments revealed that PF-mediated intestinal barrier repair and secretory normalization were operated through <em>s_Lachnospiraceae_bacterium</em> (<em>L. bacterium</em>) modulation. Functional characterization demonstrated that <em>L. bacterium</em> alleviated IBS-D pathophysiology by enhancing tight junction assembly and facilitating mucin maturation. Furthermore, mechanistic analysis identified FoxO3a signaling as a pivotal mediator of the intestinal protection conferred by<em> L. bacterium</em>. Collectively, this study not only establishes PF as a promising therapeutic candidate but also uncovers novel microbial-epithelial crosstalk mechanisms, highlighting the translational potential of probiotic-based interventions and bioactive compounds for intestinal disorders. These findings provide clinically relevant insights for the development of targeted therapeutics for IBS-D.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sciopen.com/article_pdf/1913053365120012289.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147334143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food-derived α-glucosidase (α-GLU) inhibitory peptides have emerged as promising natural agents for managing type 2 diabetes mellitus (T2DM) due to their favorable safety and bioactivity profiles. Food-derived α-glucosidase (α-GLU) inhibitory peptides have received considerable interest in managing type 2 diabetes mellitus (T2DM) due to their high safety. This study identifies high-activity α-glucosidase (α-GLU) inhibitory peptides from sturgeon fish muscle protein (SFMP) and investigates their mechanisms of action on α-GLU inhibition and enhancement of insulin-resistant HepG2 cells (IR-HepG2) through a systematic investigation. Moreover, two peptides, VLLQKNNDT (VT-9) and LSMQRAQEE (LE-9), exhibited significant α-GLU inhibitory activity and stability as confirmed by in vitro gastrointestinal digestion simulations. Molecular docking revealed that VT-9 and LE-9 interact with α-GLU active sites, induce conformational changes, and thus reduce α-GLU activity. Moreover, both peptides promoted glucose consumption and glycogen synthesis in IR-HepG2 cells, which improves insulin resistance through the modulation of insulin signaling pathways. Experimental validation confirms the hypoglycemic effects of these peptides, providing insights into SFMP development and its potential in anti-diabetic foods in managing T2DM.
{"title":"Insights into novel α-glucosidase (α-GLU) inhibitory peptides from sturgeon fish muscle protein based on in-silico and in-vitro investigation","authors":"Zhiqing Liu, Xiao‐Yan Kuang, Lingfeng Xu, Xiaodan Fan, Wen‐Yong Lou","doi":"10.1016/j.foodw.2025.100010","DOIUrl":"https://doi.org/10.1016/j.foodw.2025.100010","url":null,"abstract":"Food-derived α-glucosidase (α-GLU) inhibitory peptides have emerged as promising natural agents for managing type 2 diabetes mellitus (T2DM) due to their favorable safety and bioactivity profiles. Food-derived α-glucosidase (α-GLU) inhibitory peptides have received considerable interest in managing type 2 diabetes mellitus (T2DM) due to their high safety. This study identifies high-activity α-glucosidase (α-GLU) inhibitory peptides from sturgeon fish muscle protein (SFMP) and investigates their mechanisms of action on α-GLU inhibition and enhancement of insulin-resistant HepG2 cells (IR-HepG2) through a systematic investigation. Moreover, two peptides, VLLQKNNDT (VT-9) and LSMQRAQEE (LE-9), exhibited significant α-GLU inhibitory activity and stability as confirmed by in vitro gastrointestinal digestion simulations. Molecular docking revealed that VT-9 and LE-9 interact with α-GLU active sites, induce conformational changes, and thus reduce α-GLU activity. Moreover, both peptides promoted glucose consumption and glycogen synthesis in IR-HepG2 cells, which improves insulin resistance through the modulation of insulin signaling pathways. Experimental validation confirms the hypoglycemic effects of these peptides, providing insights into SFMP development and its potential in anti-diabetic foods in managing T2DM.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":"100010-100010"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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