Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250025
Tao Li , Guirong Zeng , Lingfeng Zhu , Yanyang Wu , Qun Zhang , Fuhua Fu , Donglin Su , Gaoyang Li , Qili Li , Yang Shan
Gut microbiota plays a crucial role in the pathophysiology of depression. This study aimed to explore the antidepressant effect of mature whole Citrus aurantium fruit extract (FEMC) in the chronic unpredictable mild stress (CUMS) model. The behavioral tests were applied to assess antidepressant effect and 16S rRNA sequencing was used to analyze the changes of gut microbiota. The results showed that the major components of FEMC were naringin and neohesperidin and significantly increased the sucrose preference index of the mice. FEMC also could reduce the feeding latency in an open field test and the rest time in a novelty suppressed feeding test. In addition, FEMC could increase CUMS-induced reduction in the levels of BDNF, PSD95, and SYN in the hippocampus. Moreover, FEMC intervention slightly decreased the ratio of Firmicutes to Bacteroidota. Meanwhile, FEMC reduced the abundance of the Prevotellaceae_Ga6A1_group, [Ruminococcus]_torques_group, which have been reported to be closely related to inflammation. Bioinformatics analysis revealed that mitogen-activated protein kinase (MAPK) signaling pathway and lipopolysaccharide biosynthesis were involved in the anti-inflammatory effect of FEMC in the CUMS animal model. Finally, the ELISA results showed that FEMC could significantly reduce the expression of pro-inflammatory cytokines IL-6 and TNF-α in the serum of depressive mice. Our results suggest FEMC can ameliorate depressive behavior by inhibiting gut microbiota-mediated inflammation in mice.
{"title":"Citrus aurantium L. extract alleviate depression by inhibiting gut microbiota-mediated inflammation in mice","authors":"Tao Li , Guirong Zeng , Lingfeng Zhu , Yanyang Wu , Qun Zhang , Fuhua Fu , Donglin Su , Gaoyang Li , Qili Li , Yang Shan","doi":"10.26599/FSHW.2023.9250025","DOIUrl":"10.26599/FSHW.2023.9250025","url":null,"abstract":"<div><div>Gut microbiota plays a crucial role in the pathophysiology of depression. This study aimed to explore the antidepressant effect of mature whole <em>Citrus aurantium</em> fruit extract (FEMC) in the chronic unpredictable mild stress (CUMS) model. The behavioral tests were applied to assess antidepressant effect and 16S rRNA sequencing was used to analyze the changes of gut microbiota. The results showed that the major components of FEMC were naringin and neohesperidin and significantly increased the sucrose preference index of the mice. FEMC also could reduce the feeding latency in an open field test and the rest time in a novelty suppressed feeding test. In addition, FEMC could increase CUMS-induced reduction in the levels of BDNF, PSD95, and SYN in the hippocampus. Moreover, FEMC intervention slightly decreased the ratio of Firmicutes to Bacteroidota. Meanwhile, FEMC reduced the abundance of the Prevotellaceae_Ga6A1_group, [<em>Ruminococcus</em>]_<em>torques</em>_group, which have been reported to be closely related to inflammation. Bioinformatics analysis revealed that mitogen-activated protein kinase (MAPK) signaling pathway and lipopolysaccharide biosynthesis were involved in the anti-inflammatory effect of FEMC in the CUMS animal model. Finally, the ELISA results showed that FEMC could significantly reduce the expression of pro-inflammatory cytokines IL-6 and TNF-α in the serum of depressive mice. Our results suggest FEMC can ameliorate depressive behavior by inhibiting gut microbiota-mediated inflammation in mice.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3403-3414"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140469503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250027
Lijie Zhu , Yueying Pan , Yingyan Li , Yingjie Zhou , He Liu , Xiuying Liu
The interaction mechanism between soyasaponin (Ssa) and bitter receptors/mucin, as well as the saliva interface behavior of Ssa, were investigated to explore the presentation mechanism of Ssa bitterness and astringency (BA). Strong bitterness arising from high Ssa concentrations (0.5–1.5 mg/mL) had a masking effect on astringency. At Ssa concentrations of 1.0–1.5 mg/mL, Ssa micelles altered the structure of mucin, exposing its internal tryptophan to a more polar environment. At Ssa concentrations of 0.05–1.50 mg/mL, its reaction with mucin increased the aggregation of particles in artificial saliva, which reduced the frictional lubricating properties of oral saliva. Ssa-mucin interactions affected the salivary interfacial adsorption layer, and their complexes synergistically reduced the interfacial tension. Ssa monomers and soyasapogenols bind to bitter receptors/mucin via hydrogen bonding and hydrophobic interactions. Class A Ssa binds more strongly than class B Ssa, and thus likely presents a higher BA. In conclusion, Ssa interacts with bitter receptors/mucin causing conformational changes and aggregation of salivary mucin, resulting in diminished frictional lubricating properties of oral saliva. This, in turn, affects taste perception and gustatory transmission.
{"title":"Molecular mechanisms of bitterness and astringency in the oral cavity induced by soyasaponin","authors":"Lijie Zhu , Yueying Pan , Yingyan Li , Yingjie Zhou , He Liu , Xiuying Liu","doi":"10.26599/FSHW.2023.9250027","DOIUrl":"10.26599/FSHW.2023.9250027","url":null,"abstract":"<div><div>The interaction mechanism between soyasaponin (Ssa) and bitter receptors/mucin, as well as the saliva interface behavior of Ssa, were investigated to explore the presentation mechanism of Ssa bitterness and astringency (BA). Strong bitterness arising from high Ssa concentrations (0.5–1.5 mg/mL) had a masking effect on astringency. At Ssa concentrations of 1.0–1.5 mg/mL, Ssa micelles altered the structure of mucin, exposing its internal tryptophan to a more polar environment. At Ssa concentrations of 0.05–1.50 mg/mL, its reaction with mucin increased the aggregation of particles in artificial saliva, which reduced the frictional lubricating properties of oral saliva. Ssa-mucin interactions affected the salivary interfacial adsorption layer, and their complexes synergistically reduced the interfacial tension. Ssa monomers and soyasapogenols bind to bitter receptors/mucin via hydrogen bonding and hydrophobic interactions. Class A Ssa binds more strongly than class B Ssa, and thus likely presents a higher BA. In conclusion, Ssa interacts with bitter receptors/mucin causing conformational changes and aggregation of salivary mucin, resulting in diminished frictional lubricating properties of oral saliva. This, in turn, affects taste perception and gustatory transmission.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3424-3433"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140466505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250031
Yanfang Wu , Jing Liu , Dantong Liu , Menglei Xia , Jia Song , Kai Liang , Chaochun Li , Yu Zheng , Min Wang
Shanxi aged vinegar (SAV) is a famous cereal vinegar in China, which is produced through a solid-state fermentation where diverse microbes spontaneously and complex interactions occur. Here, combined with the metatranscriptomics, the microbial co-occurrence network was constructed, indicating that Lactobacillus, Acetobacter and Pediococcus are the most critical genera to maintain the fermentation stability. Based on an extensive collection of 264 relevant literatures, a transport network containing 2271 reactions between microorganisms and compounds was constructed, showing that glucose (84 % of all species), fructose (67 %) and maltose (67 %) are the most frequently utilized substrates while lactic acid (64 %), acetic acid (45 %) are the most frequently occurring metabolites. Specifically, the metabolic influence of species pairs was calculated using a mathematical calculation model and the metabolic influence network was constructed. The topology properties analysis found that Lactobacillus was the key role with robust metabolic control of vinegar fermentation ecosystem and acetic acid and lactic acid were the main metabolites with feedback regulation in microbial metabolism of SAV. Furthermore, systematic coordination of positive and negative impacts was proved to be inevitable to form flavor compounds and maintain a natural microbial ecosystem. This study provides a new perspective for understanding microbial interactions in fermented food.
{"title":"Microbial metabolic interaction in fermentation ecosystem and cooperation in flavor compounds formation of Chinese cereal vinegar","authors":"Yanfang Wu , Jing Liu , Dantong Liu , Menglei Xia , Jia Song , Kai Liang , Chaochun Li , Yu Zheng , Min Wang","doi":"10.26599/FSHW.2023.9250031","DOIUrl":"10.26599/FSHW.2023.9250031","url":null,"abstract":"<div><div>Shanxi aged vinegar (SAV) is a famous cereal vinegar in China, which is produced through a solid-state fermentation where diverse microbes spontaneously and complex interactions occur. Here, combined with the metatranscriptomics, the microbial co-occurrence network was constructed, indicating that <em>Lactobacillus</em>, <em>Acetobacter</em> and <em>Pediococcus</em> are the most critical genera to maintain the fermentation stability. Based on an extensive collection of 264 relevant literatures, a transport network containing 2271 reactions between microorganisms and compounds was constructed, showing that glucose (84 % of all species), fructose (67 %) and maltose (67 %) are the most frequently utilized substrates while lactic acid (64 %), acetic acid (45 %) are the most frequently occurring metabolites. Specifically, the metabolic influence of species pairs was calculated using a mathematical calculation model and the metabolic influence network was constructed. The topology properties analysis found that <em>Lactobacillus</em> was the key role with robust metabolic control of vinegar fermentation ecosystem and acetic acid and lactic acid were the main metabolites with feedback regulation in microbial metabolism of SAV. Furthermore, systematic coordination of positive and negative impacts was proved to be inevitable to form flavor compounds and maintain a natural microbial ecosystem. This study provides a new perspective for understanding microbial interactions in fermented food.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3472-3481"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140469337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250017
Hoon Kim , Jongyeob Kim , Byungdoo Hwang , Sang Yong Park , Ji-Yeon Shin , Eun Byeol Go , Jae Sil Kim , Youngjin Roh , Soon Chul Myung , Seok-Joong Yun , Yung Hyun Choi , Wun-Jae Kim , Sung-Kwon Moon
Krill oil (KO) exhibits various biological activities, such as anti-inflammatory and antitumor effects. However, the inhibitory effects of benign prostatic hyperplasia (BPH) in vitro and in vivo have not yet been studied. This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method. KO treatment inhibited the proliferation of WMPY-1 and BPH-1 cells by induction of G0/G1 phase arrest through the modulation of positive and negative regulators in both prostate cell types. KO treatment stimulated phosphorylation of c-Jun N-terminal kinase (JNK) and p38 signaling. In addition, KO changed the expression of BPH-related markers (5α-reductase, androgen receptor, FGF, Bcl-2, and Bax) and the activity of the proliferation-mediated NF-κB binding motif. KO-induced levels of proliferation-mediated molecules of prostate cells were attenuated in the presence of siRNA-specific p-38 (si-p38) and JNK (si-JNK). Furthermore, the administration of KO alleviated prostate size and weight and the cell layer thickness of prostate glands in a testosterone enanthate-induced BPH rat model. KO treatment altered the level of dihydrotestosterone in serum and the expression levels of BPH-related markers in prostate tissues. Finally, KO-mediated inhibition of prostatic growth was validated by histological analysis. These results suggest that KO has an inhibitory effect on BPH in prostate cells in vitro and in vivo. Thus, KO might be a potential prophylactic or therapeutic agent for patients with BPH.
{"title":"Krill oil ameliorates benign prostatic hyperplasia by regulating G1-phase cell cycle arrest and altering signaling pathways and benign prostatic hyperplasia-associated markers","authors":"Hoon Kim , Jongyeob Kim , Byungdoo Hwang , Sang Yong Park , Ji-Yeon Shin , Eun Byeol Go , Jae Sil Kim , Youngjin Roh , Soon Chul Myung , Seok-Joong Yun , Yung Hyun Choi , Wun-Jae Kim , Sung-Kwon Moon","doi":"10.26599/FSHW.2023.9250017","DOIUrl":"10.26599/FSHW.2023.9250017","url":null,"abstract":"<div><div>Krill oil (KO) exhibits various biological activities, such as anti-inflammatory and antitumor effects. However, the inhibitory effects of benign prostatic hyperplasia (BPH) <em>in vitro</em> and <em>in vivo</em> have not yet been studied. This study investigated the anti-BPH effects of KO extracted by an enzymatic hydrolysis method. KO treatment inhibited the proliferation of WMPY-1 and BPH-1 cells by induction of G<sub>0</sub>/G<sub>1</sub> phase arrest through the modulation of positive and negative regulators in both prostate cell types. KO treatment stimulated phosphorylation of c-Jun N-terminal kinase (JNK) and p38 signaling. In addition, KO changed the expression of BPH-related markers (5<em>α</em>-reductase, androgen receptor, FGF, Bcl-2, and Bax) and the activity of the proliferation-mediated NF-κB binding motif. KO-induced levels of proliferation-mediated molecules of prostate cells were attenuated in the presence of siRNA-specific p-38 (si-p38) and JNK (si-JNK). Furthermore, the administration of KO alleviated prostate size and weight and the cell layer thickness of prostate glands in a testosterone enanthate-induced BPH rat model. KO treatment altered the level of dihydrotestosterone in serum and the expression levels of BPH-related markers in prostate tissues. Finally, KO-mediated inhibition of prostatic growth was validated by histological analysis. These results suggest that KO has an inhibitory effect on BPH in prostate cells <em>in vitro</em> and <em>in vivo</em>. Thus, KO might be a potential prophylactic or therapeutic agent for patients with BPH.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3311-3324"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140469400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2024.9250193
Qian Liu , Yan Liu , Di Yang , Yanpin Liu , Yan Liu , Weicang Qiao , Juncai Hou , Yaling Wang , Minghui Zhang , Kai Yang , Xiaofei Fan , Ziqi Li , Junying Zhao , Lijun Chen
Prenatal overweight/obesity (OW/OB) can alter colostrum lipid patterns, thereby affecting the lipid metabolism and even the cognitive and healthy development of infants. However, studies on changes in colostrum lipids in the context of OW/OB are limited, particularly for glycerides and polar lipids. Therefore, this study investigated the influence of maternal prenatal weight on colostrum in lipid subclasses and molecular species. The concentration of triacylglycerols (TAGs) in the colostrum of the OW/OB group (35 894.43 mg/L) was higher than that of the normal weight (NW) group (26 639.20 mg/L), suggesting that colostrum from OW/OB mothers could provide more energy to their infants. Further analysis of the fatty acid composition of TAGs revealed that elevated maternal body weight enhanced the concentration of TAGs containing saturated or n-6 fatty acids and shortened the carbon number of TAGs. Docosahexaenoic acid (DHA)/arachidonic acid (AA)/choline-containing lipids, such as DHA-containing TAGs, AA/DHA-containing phosphatidylethanolamine, and choline-containing phospholipids, were present in higher levels in the colostrum of OW/OB mothers than NW mothers. However, the concentrations of palmitic acid-containing TAGs, linoleic acid-containing TAGs, dihomo-γ-linolenic acid-containing TAGs, and polar lipids and the ratio of TAGs containing n-6 fatty acid/n-3 fatty acid were significantly higher in the colostrum of OW/OB mothers than in that of NW mothers. The fatty acid composition and sphingoid bases of sphingolipids were also altered due to elevated body weight. In conclusion, OW/OB affects colostrum lipids with respect to composition, concentration, and percentage. Although the colostrum of healthy OW/OB mothers can provide sufficient DHA/AA/choline-containing lipids to their infants, normalization of body weight and fat reserves should be considered as a strategy for high-quality human milk lipids.
{"title":"Effects of excessive body fat on colostrum lipid patterns: overweight/obese vs. normal weight mothers","authors":"Qian Liu , Yan Liu , Di Yang , Yanpin Liu , Yan Liu , Weicang Qiao , Juncai Hou , Yaling Wang , Minghui Zhang , Kai Yang , Xiaofei Fan , Ziqi Li , Junying Zhao , Lijun Chen","doi":"10.26599/FSHW.2024.9250193","DOIUrl":"10.26599/FSHW.2024.9250193","url":null,"abstract":"<div><div>Prenatal overweight/obesity (OW/OB) can alter colostrum lipid patterns, thereby affecting the lipid metabolism and even the cognitive and healthy development of infants. However, studies on changes in colostrum lipids in the context of OW/OB are limited, particularly for glycerides and polar lipids. Therefore, this study investigated the influence of maternal prenatal weight on colostrum in lipid subclasses and molecular species. The concentration of triacylglycerols (TAGs) in the colostrum of the OW/OB group (35 894.43 mg/L) was higher than that of the normal weight (NW) group (26 639.20 mg/L), suggesting that colostrum from OW/OB mothers could provide more energy to their infants. Further analysis of the fatty acid composition of TAGs revealed that elevated maternal body weight enhanced the concentration of TAGs containing saturated or <em>n</em>-6 fatty acids and shortened the carbon number of TAGs. Docosahexaenoic acid (DHA)/arachidonic acid (AA)/choline-containing lipids, such as DHA-containing TAGs, AA/DHA-containing phosphatidylethanolamine, and choline-containing phospholipids, were present in higher levels in the colostrum of OW/OB mothers than NW mothers. However, the concentrations of palmitic acid-containing TAGs, linoleic acid-containing TAGs, dihomo-<em>γ</em>-linolenic acid-containing TAGs, and polar lipids and the ratio of TAGs containing <em>n</em>-6 fatty acid/<em>n</em>-3 fatty acid were significantly higher in the colostrum of OW/OB mothers than in that of NW mothers. The fatty acid composition and sphingoid bases of sphingolipids were also altered due to elevated body weight. In conclusion, OW/OB affects colostrum lipids with respect to composition, concentration, and percentage. Although the colostrum of healthy OW/OB mothers can provide sufficient DHA/AA/choline-containing lipids to their infants, normalization of body weight and fat reserves should be considered as a strategy for high-quality human milk lipids.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3708-3717"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143163145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250018
Kai Wang , Zixin Fu , Yuqing Tan , Hui Hong , Jianping Wu , Yongkang Luo
Atherosclerosis (AS) is a major cause of cardiovascular diseases (CVDs) and a strong link with hepatic steatosis. Silver carp muscle hydrolysate (SCH) possess various beneficial activities but its effect on AS and hepatic steatosis is yet unknown. This study aimed to investigate the effects of SCH on AS lesions and hepatic steatosis using apoE-/- mice. Results showed that SCH significantly reduced the vascular AS plaques and alleviated hepatic steatosis lesions in apoE-/- mice. Consistent with this, the lipid levels both in circulation and liver were lowered by SCH. The mechanism analysis showed SCH down-regulated the expression of genes involved in lipoproteins production while up-regulated the expression of genes related to reverse cholesterol transport (RCT) in liver. Meanwhile, SCH remarkably promoted transintestinal cholesterol excretion (TICE) process in intestine, partly contributing to the reduction of blood lipids. The peptide profile data indicated LYF, HWPW, FPK, and YPR are the main peptides in SCH that play a vital role in alleviating AS lesions and hepatic steatosis. Our findings provided new knowledge for the application of SCH in ameliorating CVDs and liver diseases.
{"title":"Silver carp muscle hydrolysate ameliorated atherosclerosis and liver injury in apoE-/- mice: the modulator effects on enterohepatic cholesterol metabolism","authors":"Kai Wang , Zixin Fu , Yuqing Tan , Hui Hong , Jianping Wu , Yongkang Luo","doi":"10.26599/FSHW.2023.9250018","DOIUrl":"10.26599/FSHW.2023.9250018","url":null,"abstract":"<div><div>Atherosclerosis (AS) is a major cause of cardiovascular diseases (CVDs) and a strong link with hepatic steatosis. Silver carp muscle hydrolysate (SCH) possess various beneficial activities but its effect on AS and hepatic steatosis is yet unknown. This study aimed to investigate the effects of SCH on AS lesions and hepatic steatosis using apoE<sup>-/-</sup> mice. Results showed that SCH significantly reduced the vascular AS plaques and alleviated hepatic steatosis lesions in apoE<sup>-/-</sup> mice. Consistent with this, the lipid levels both in circulation and liver were lowered by SCH. The mechanism analysis showed SCH down-regulated the expression of genes involved in lipoproteins production while up-regulated the expression of genes related to reverse cholesterol transport (RCT) in liver. Meanwhile, SCH remarkably promoted transintestinal cholesterol excretion (TICE) process in intestine, partly contributing to the reduction of blood lipids. The peptide profile data indicated LYF, HWPW, FPK, and YPR are the main peptides in SCH that play a vital role in alleviating AS lesions and hepatic steatosis. Our findings provided new knowledge for the application of SCH in ameliorating CVDs and liver diseases.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3325-3338"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140463012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250021
Jian Nan , Jinglei Li , Haishan Wu , Haoran Cheng , Hyun Jin Park , Qingsheng Zhao , Liu Yang
Crocetin displays strong antioxidant, anti-inflammatory and anti-depression activity which is promising to relieve symptoms of fatigue. As a carotenoid, crocetin is difficult to dissolve in water and highly unstable against many environmental factors. Nanoliposome is used to encapsulate crocetin to improve its water dispersion. In the present study, the antifatigue activities and potential mechanism of crocetin loaded nanoliposome (CLN) was extensively investigated. The potential antifatigue pathway of CLN was analyzed. Furthermore, impact of CLN on the gut microbiota structure was examined which contributes to its antifatigue functions. CLN significantly increases exhaustive swimming time of fatigue mice, decreases the blood contents of lactic, blood urea nitrogen (BUN) and malondialdehyde (MDA). At the same time, CLN improves the activity of glutathione peroxidase (GSH-Px) and succinate dehydrogenase (SDH) enzyme, attenuates the oxidant stress in mice. CLN activates the Activated adenosine monophosphate-activated kinase (AMPK)/peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) signaling pathway of fatigue mice, increases the mRNA expression of ATP synthase. It also increases mRNA expression of mitochondrial transcription factor A (TFAM) which promotes mitochondrial biogenesis. Additionally, CLN ameliorates the gut microbiota structure by increasing the abundance of genus such as Lactobacillus in fatigue mice. In summary, CLN exerts strong anti-fatigue properties by decreasing the oxidant stress and the contents of harmful metabolites, augmenting the production of ATP, and potentially ameliorating the gut microbiota structure.
{"title":"Anti-fatigue activity and mechanism of crocetin loaded nanoliposome in acute exercise-treated mice","authors":"Jian Nan , Jinglei Li , Haishan Wu , Haoran Cheng , Hyun Jin Park , Qingsheng Zhao , Liu Yang","doi":"10.26599/FSHW.2023.9250021","DOIUrl":"10.26599/FSHW.2023.9250021","url":null,"abstract":"<div><div>Crocetin displays strong antioxidant, anti-inflammatory and anti-depression activity which is promising to relieve symptoms of fatigue. As a carotenoid, crocetin is difficult to dissolve in water and highly unstable against many environmental factors. Nanoliposome is used to encapsulate crocetin to improve its water dispersion. In the present study, the antifatigue activities and potential mechanism of crocetin loaded nanoliposome (CLN) was extensively investigated. The potential antifatigue pathway of CLN was analyzed. Furthermore, impact of CLN on the gut microbiota structure was examined which contributes to its antifatigue functions. CLN significantly increases exhaustive swimming time of fatigue mice, decreases the blood contents of lactic, blood urea nitrogen (BUN) and malondialdehyde (MDA). At the same time, CLN improves the activity of glutathione peroxidase (GSH-Px) and succinate dehydrogenase (SDH) enzyme, attenuates the oxidant stress in mice. CLN activates the Activated adenosine monophosphate-activated kinase (AMPK)/peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) signaling pathway of fatigue mice, increases the mRNA expression of ATP synthase. It also increases mRNA expression of mitochondrial transcription factor A (TFAM) which promotes mitochondrial biogenesis. Additionally, CLN ameliorates the gut microbiota structure by increasing the abundance of genus such as <em>Lactobacillus</em> in fatigue mice. In summary, CLN exerts strong anti-fatigue properties by decreasing the oxidant stress and the contents of harmful metabolites, augmenting the production of ATP, and potentially ameliorating the gut microbiota structure.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3361-3370"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140463092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250013
Inés Domínguez-López , Isabella Parilli-Moser , Anna Vallverdú-Queralt , Anna Tresserra-Rimbau , Cinta Valls-Pedret , Zenaida Vázquez-Ruiz , Olga Castañer , Ramon Estruch , Emili Ros , Rosa M. Lamuela-Raventós
With increasing life expectancy, neurodegenerative diseases have become one of the leading causes of ill-health in the elderly. Preventive strategies include following healthy diets, such as the Mediterranean diet, which is particularly rich in polyphenols, bioactive compounds with neuroprotective properties. The aim of this study was to assess the association of microbial phenolic metabolites (MPM) with cognition. This cross-sectional analysis was performed with 200 participants of the PREDIMED trial (Barcelona-Clinic recruitment center). A novel method based on liquid chromatography coupled to mass spectrometry was used to identify urinary MPM (protocatechuic acid, enterodiol glucuronide, enterolactone glucuronide, urolithin B glucuronide, and vanillic acid glucuronide), and cognitive function was evaluated with neuropsychological tests. Multivariable-adjusted ordinary least squares regression was used to assess the associations between cognitive function and MPM, and a score was calculated as the weighted sum of MPM. A higher MPM score was associated with better frontal lobe function. Among individual metabolites, vanillic acid glucuronide was correlated with frontal cognitive performance. Participants with higher concentrations of vanillic acid glucuronide and urolithin B glucuronide obtained better scores in the Color Trail Test part 2. A higher score for urinary multi-MPM was associated with better frontal cognitive performance in an older Mediterranean population.
{"title":"Microbial phenolic metabolites are associated with better frontal lobe cognition","authors":"Inés Domínguez-López , Isabella Parilli-Moser , Anna Vallverdú-Queralt , Anna Tresserra-Rimbau , Cinta Valls-Pedret , Zenaida Vázquez-Ruiz , Olga Castañer , Ramon Estruch , Emili Ros , Rosa M. Lamuela-Raventós","doi":"10.26599/FSHW.2023.9250013","DOIUrl":"10.26599/FSHW.2023.9250013","url":null,"abstract":"<div><div>With increasing life expectancy, neurodegenerative diseases have become one of the leading causes of ill-health in the elderly. Preventive strategies include following healthy diets, such as the Mediterranean diet, which is particularly rich in polyphenols, bioactive compounds with neuroprotective properties. The aim of this study was to assess the association of microbial phenolic metabolites (MPM) with cognition. This cross-sectional analysis was performed with 200 participants of the PREDIMED trial (Barcelona-Clinic recruitment center). A novel method based on liquid chromatography coupled to mass spectrometry was used to identify urinary MPM (protocatechuic acid, enterodiol glucuronide, enterolactone glucuronide, urolithin B glucuronide, and vanillic acid glucuronide), and cognitive function was evaluated with neuropsychological tests. Multivariable-adjusted ordinary least squares regression was used to assess the associations between cognitive function and MPM, and a score was calculated as the weighted sum of MPM. A higher MPM score was associated with better frontal lobe function. Among individual metabolites, vanillic acid glucuronide was correlated with frontal cognitive performance. Participants with higher concentrations of vanillic acid glucuronide and urolithin B glucuronide obtained better scores in the Color Trail Test part 2. A higher score for urinary multi-MPM was associated with better frontal cognitive performance in an older Mediterranean population.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3266-3272"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140464725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250029
Xinqi Chen , Yang Chen , Catherine Stanton , R. Paul Ross , Jianxin Zhao , Bo Yang , Wei Chen
This study aimed to evaluate the effects of Bifidobacterium breve CCFM683 on psoriasis and to investigate the underlying mechanisms. B. breve CCFM683 significantly ameliorated psoriasis in mice as well as elevated the deoxycholic acid (DCA) and lithocholic acid (LCA) in the colon compared with those of the imiquimod (IMQ)-treated mice. Meanwhile, B. breve CCFM683 increased the relative abundance of DCA-producing Lachnoclostridium and diminished the harmful Desulfovibrio and Prevotellaceae UCG001. Additionally, the farnesoid X receptor (FXR) in the skin was activated and the expression of the Toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) pathway was inhibited, and the downstream interleukin (IL)-17 and tumor necrosis factor (TNF)-α were downregulated whereas IL-10 was up-regulated. Moreover, the subsequent hyperproliferation of keratinocytes and the dysfunction of the epidermal barrier were improved. In conclusion, CCFM683 administration ameliorated IMQ-induced psoriasis via modulating gut microbiota, promoting the DCA production, regulating the FXR-TLR4/NF-κB pathway, diminishing proinflammatory cytokines, and regulating keratinocytes and epidermal barrier. These findings may be conducive to elucidating the mechanism for probiotics to ameliorate psoriasis and to promote its clinical trials in skin disease.
本研究旨在评价短双歧杆菌CCFM683对银屑病的治疗作用，并探讨其作用机制。与咪喹莫特（IMQ）处理的小鼠相比，短芽孢杆菌CCFM683显著改善小鼠牛皮癣，并提高结肠中去氧胆酸（DCA）和石胆酸（LCA）。同时，B. breve CCFM683增加了产dca的Lachnoclostridium的相对丰度，减少了有害的Desulfovibrio和Prevotellaceae UCG001。激活皮肤法尼松X受体（FXR），抑制toll样受体4 (TLR4)/核因子κ b （NF-κB）通路表达，下调下游白细胞介素(IL)-17和肿瘤坏死因子(TNF)-α，上调IL-10。此外，随后的角化细胞过度增殖和表皮屏障功能障碍得到改善。综上所述，CCFM683通过调节肠道菌群、促进DCA生成、调节FXR-TLR4/NF-κB通路、减少促炎细胞因子、调节角化细胞和表皮屏障来改善imq诱导的银屑病。这些发现可能有助于阐明益生菌改善银屑病的机制，并促进其在皮肤病中的临床试验。
{"title":"Protective effects of Bifidobacterium breve on imiquimod-induced psoriasis in mice through secondary bile acid production and FXR-TLR4/NF-κB pathway","authors":"Xinqi Chen , Yang Chen , Catherine Stanton , R. Paul Ross , Jianxin Zhao , Bo Yang , Wei Chen","doi":"10.26599/FSHW.2023.9250029","DOIUrl":"10.26599/FSHW.2023.9250029","url":null,"abstract":"<div><div>This study aimed to evaluate the effects of <em>Bifidobacterium breve</em> CCFM683 on psoriasis and to investigate the underlying mechanisms. <em>B. breve</em> CCFM683 significantly ameliorated psoriasis in mice as well as elevated the deoxycholic acid (DCA) and lithocholic acid (LCA) in the colon compared with those of the imiquimod (IMQ)-treated mice. Meanwhile, <em>B. breve</em> CCFM683 increased the relative abundance of DCA-producing <em>Lachnoclostridium</em> and diminished the harmful <em>Desulfovibrio</em> and Prevotellaceae UCG001. Additionally, the farnesoid X receptor (FXR) in the skin was activated and the expression of the Toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) pathway was inhibited, and the downstream interleukin (IL)-17 and tumor necrosis factor (TNF)-α were downregulated whereas IL-10 was up-regulated. Moreover, the subsequent hyperproliferation of keratinocytes and the dysfunction of the epidermal barrier were improved. In conclusion, CCFM683 administration ameliorated IMQ-induced psoriasis via modulating gut microbiota, promoting the DCA production, regulating the FXR-TLR4/NF-κB pathway, diminishing proinflammatory cytokines, and regulating keratinocytes and epidermal barrier. These findings may be conducive to elucidating the mechanism for probiotics to ameliorate psoriasis and to promote its clinical trials in skin disease.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3447-3460"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140466773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01DOI: 10.26599/FSHW.2023.9250043
Xiujuan Wang , Haiyue Sun , Jian Wu , Yu Wang , Zhiyi Ai , Xinzhu Wang , Bo Nan , Yong Cao , Xia Li , Jingsheng Liu , Yuhua Wang
Trehalose (TRE) was used to improve the gastrointestinal tolerance of Lactobacillus plantarum embedded with whey protein concentrate/pullulan (WPC/PUL) hydrogel and the embedded L. plantarum was applied to juice. The study indicated that 5 % TRE significantly increased the viable counts of L. plantarum embedded in WPC/PUL hydrogel from (8.83 ± 0.03) to (9.14 ± 0.04) (lg (CFU/g)) in simulated gastric juice (SGJ) and from (9.13 ± 0.04) to (9.38 ± 0.04) (lg (CFU/g)) in simulated intestinal juice, respectively. The addition of TRE improved the glass transition temperature of WPC/PUL hydrogel and decreased the hardness and its solubility in SGJ, which may be responsible for the improved protection of WPC/PUL hydrogels on L. plantarum. In addition, TRE increased the viable counts of L. plantarum in WPC/PUL probiotic microcapsule juice at low pH and high temperature during storage.
{"title":"Improved viability of trehalose on Lactobacillus plantarum embedded with whey protein concentrate/pullulan hydrogel in simulated gastrointestinal conditions and its application in juice","authors":"Xiujuan Wang , Haiyue Sun , Jian Wu , Yu Wang , Zhiyi Ai , Xinzhu Wang , Bo Nan , Yong Cao , Xia Li , Jingsheng Liu , Yuhua Wang","doi":"10.26599/FSHW.2023.9250043","DOIUrl":"10.26599/FSHW.2023.9250043","url":null,"abstract":"<div><div>Trehalose (TRE) was used to improve the gastrointestinal tolerance of <em>Lactobacillus plantarum</em> embedded with whey protein concentrate/pullulan (WPC/PUL) hydrogel and the embedded <em>L. plantarum</em> was applied to juice. The study indicated that 5 % TRE significantly increased the viable counts of <em>L. plantarum</em> embedded in WPC/PUL hydrogel from (8.83 ± 0.03) to (9.14 ± 0.04) (lg (CFU/g)) in simulated gastric juice (SGJ) and from (9.13 ± 0.04) to (9.38 ± 0.04) (lg (CFU/g)) in simulated intestinal juice, respectively. The addition of TRE improved the glass transition temperature of WPC/PUL hydrogel and decreased the hardness and its solubility in SGJ, which may be responsible for the improved protection of WPC/PUL hydrogels on <em>L. plantarum</em>. In addition, TRE increased the viable counts of <em>L. plantarum</em> in WPC/PUL probiotic microcapsule juice at low pH and high temperature during storage.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3614-3623"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140466827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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