Pub Date : 2025-02-01DOI: 10.26599/fshw.2025.9250470
Yingying Sun, Yana Liu, Fei Jia, Han Wang, Yuhan Wang, Yi Liu, Xingmin Li, Ruitong Dai
Bacillus cereus spores pose a potential threat to various food products, including meat, grain, vegetables, and dairy products, due to their remarkable resistance to environmental stress. Ohmic heating (OH) is an emerging alternative technology for inactivating spores. This study aimed to investigate the effects of OH on Bacillus cereus spores, specifically examining its impact on heat resistance, leakage of intracellular substances, integrity of the cortex and membrane, and structural changes, in order to elucidate the potential inactivation mechanism of OH. The results demonstrated the efficacy of OH in inactivating B. cereus spores in various liquid food products while maintaining product quality. The wet heat resistance of spores decreased after OH treatments at low-temperatures (60-70 ℃), potentially promoting subsequent inactivation. Conversely, resistance increased following OH treatments at higher temperatures and oil bath heating (OB) treatments. Similar inactivation effects were observed between OB and OH when the temperature reached 80 ℃ and 98 ℃. Both OH and OB treatments damaged spore structures, resulting in leakage of intracellular substances and disruption of homeostasis. However, OH treatment at 10 V/cm and 50 Hz resulted in less leakage of dipicolinic acid, protein, anucleic acids within the spores, and appeared to have additional effects on the structures within the inner membrane. These findings could facilitate the development of OH as a promising technology for effective spore control in food.
{"title":"Insight into the inactivation of <em>Bacillus cereus</em> spores by ohmic heating and application in liquid food","authors":"Yingying Sun, Yana Liu, Fei Jia, Han Wang, Yuhan Wang, Yi Liu, Xingmin Li, Ruitong Dai","doi":"10.26599/fshw.2025.9250470","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250470","url":null,"abstract":"<em>Bacillus cereus</em> spores pose a potential threat to various food products, including meat, grain, vegetables, and dairy products, due to their remarkable resistance to environmental stress. Ohmic heating (OH) is an emerging alternative technology for inactivating spores. This study aimed to investigate the effects of OH on <em>Bacillus cereus</em> spores, specifically examining its impact on heat resistance, leakage of intracellular substances, integrity of the cortex and membrane, and structural changes, in order to elucidate the potential inactivation mechanism of OH. The results demonstrated the efficacy of OH in inactivating <em>B. cereus</em> spores in various liquid food products while maintaining product quality. The wet heat resistance of spores decreased after OH treatments at low-temperatures (60-70 ℃), potentially promoting subsequent inactivation. Conversely, resistance increased following OH treatments at higher temperatures and oil bath heating (OB) treatments. Similar inactivation effects were observed between OB and OH when the temperature reached 80 ℃ and 98 ℃. Both OH and OB treatments damaged spore structures, resulting in leakage of intracellular substances and disruption of homeostasis. However, OH treatment at 10 V/cm and 50 Hz resulted in less leakage of dipicolinic acid, protein, anucleic acids within the spores, and appeared to have additional effects on the structures within the inner membrane. These findings could facilitate the development of OH as a promising technology for effective spore control in food.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/local/article_pdf/10.26599/FSHW.2025.9250470.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Advanced glycation end products (AGEs) are harmful molecules formed through non-enzymatic reactions between proteins, lipids, and reducing sugars, contributing to diseases such as diabetes, Alzheimer’s, and cardiovascular conditions. This study investigates the inhibitory mechanisms of CMP-LSOPC nanoparticles (NPs) on AGEs release during gastrointestinal digestion. CMP-LSOPC NPs were synthesized by complexing carboxymethyl pachymaran (CMP) with lotus seedpod oligomeric procyanidins (LSOPC), its structure confirmed via FTIR, UV-Vis, SEM, and DSC analyses. In simulated gastrointestinal conditions, CMP-LSOPC NPs exhibited a significant reduction in AGE formation, achieving up to lowering AGE release by 48.5% compared to LSOPC alone. Furthermore, associated mechanisms are explored, including CMP-LSOPC NPs improving the stability, and antioxidant activity of LSOPC, inhibiting the activity of related hydrolase enzymes in the gastrointestinal environment. The CMP-LSOPC NPs group exhibited 4.1% higher LSOPC content during the gastric phase compared to LSOPC alone, indicating that CMP-LSOPC NPs having better stability. The antioxidant activity, measured through DPPH, ABTS+, and hydroxyl radical scavenging assays, demonstrated that CMP-LSOPC NPs enhanced antioxidant capacity, with a 35% increase in DPPH radical scavenging and 29% increase in ABTS+ radical scavenging compared to LSOPC alone. Enzyme inhibition assays showed a protective effect, with a 22% decrease in trypsin activity and 19% reduction in pepsin activity. Meanwhile, mass spectrometry revealed the presence of more long-chain glycopeptides in the CMP-LSOPC NPs group, which may exert beneficial influence on adiminishing the absorption of harmful AGEs. However, the potential risks of accumulating long glycated peptides in the colon should not be overlooked. Overall, CMP-LSOPC NPs effectively inhibited AGE release, which may offer a promising strategy for reducing dietary AGEs harm.
{"title":"Inhibitory mechanism of CMP-LSOPC nanoparticles on advanced glycation end products released during digestion","authors":"Wen‐Jun Li, Mengyao Niu, Chenxu Bao, Zihao Gong, Xiaozhi Ming, Haiyin Zheng, Ping Wu, Cui Cheng, Wei Li, Qian Wu","doi":"10.26599/fshw.2025.9250481","DOIUrl":"https://doi.org/10.26599/fshw.2025.9250481","url":null,"abstract":"Advanced glycation end products (AGEs) are harmful molecules formed through non-enzymatic reactions between proteins, lipids, and reducing sugars, contributing to diseases such as diabetes, Alzheimer’s, and cardiovascular conditions. This study investigates the inhibitory mechanisms of CMP-LSOPC nanoparticles (NPs) on AGEs release during gastrointestinal digestion. CMP-LSOPC NPs were synthesized by complexing carboxymethyl pachymaran (CMP) with lotus seedpod oligomeric procyanidins (LSOPC), its structure confirmed via FTIR, UV-Vis, SEM, and DSC analyses. In simulated gastrointestinal conditions, CMP-LSOPC NPs exhibited a significant reduction in AGE formation, achieving up to lowering AGE release by 48.5% compared to LSOPC alone. Furthermore, associated mechanisms are explored, including CMP-LSOPC NPs improving the stability, and antioxidant activity of LSOPC, inhibiting the activity of related hydrolase enzymes in the gastrointestinal environment. The CMP-LSOPC NPs group exhibited 4.1% higher LSOPC content during the gastric phase compared to LSOPC alone, indicating that CMP-LSOPC NPs having better stability. The antioxidant activity, measured through DPPH, ABTS+, and hydroxyl radical scavenging assays, demonstrated that CMP-LSOPC NPs enhanced antioxidant capacity, with a 35% increase in DPPH radical scavenging and 29% increase in ABTS+ radical scavenging compared to LSOPC alone. Enzyme inhibition assays showed a protective effect, with a 22% decrease in trypsin activity and 19% reduction in pepsin activity. Meanwhile, mass spectrometry revealed the presence of more long-chain glycopeptides in the CMP-LSOPC NPs group, which may exert beneficial influence on adiminishing the absorption of harmful AGEs. However, the potential risks of accumulating long glycated peptides in the colon should not be overlooked. Overall, CMP-LSOPC NPs effectively inhibited AGE release, which may offer a promising strategy for reducing dietary AGEs harm.","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/local/article_pdf/10.26599/FSHW.2025.9250481.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01Epub Date: 2024-12-18DOI: 10.26599/FSHW.2023.9250048
Zhiyu Zhang , Hualing Xie , Mohamed A. Farag , Zhenhao Li , Qingping Wu , Ping Shao
Gastric ulcer is a widespread disease caused by various etiologies. Dendrobium officinale flowers exert several health benefits owing to their rich flavonoid content. In this study, protective effects and possible action mechanisms of D. officinale flowers’ flavonoid enriched extract (DOFF) were assessed against gastric ulcer. The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34 % of the total extractive was flavonoid, and ultra-high performance liquid chromatography time of flight mass spectrometer (UPLC-Q-TOF/MS) revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids. In addition, in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions, with drop-in ulcer index from 64.33 ± 8.76 to 32.00 ± 4.47. Similar results were revealed in human gastric mucosal epithelia cells, with cells viability to increase from 27.2 % to 61.6 % post DOFF administration. To analyzed the protect effect of DOFF, we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways. The results showed that DOFF (320 μg/mL) could increase the level of oxidation marker protein (HO-1), apoptosis regulatory protein (Bcl-2) and autophagy marker (LC3β) by 50.84 %, 43.85 %, and 59.21 % compared with ethanol-treated group respectively. Further analyzed of the mitochondrial activity and apoptosis pathway, we found that DOFF appeared to mitigate against ethanol-induced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.
{"title":"Dendrobium officinale flowers flavonoids enriched extract protects against acute ethanol-induced gastric ulcers via AMPK/PI3K signaling pathways","authors":"Zhiyu Zhang , Hualing Xie , Mohamed A. Farag , Zhenhao Li , Qingping Wu , Ping Shao","doi":"10.26599/FSHW.2023.9250048","DOIUrl":"10.26599/FSHW.2023.9250048","url":null,"abstract":"<div><div>Gastric ulcer is a widespread disease caused by various etiologies. <em>Dendrobium officinale</em> flowers exert several health benefits owing to their rich flavonoid content. In this study, protective effects and possible action mechanisms of <em>D. officinale</em> flowers’ flavonoid enriched extract (DOFF) were assessed against gastric ulcer. The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34 % of the total extractive was flavonoid, and ultra-high performance liquid chromatography time of flight mass spectrometer (UPLC-Q-TOF/MS) revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids. In addition, <em>in vivo</em> assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions, with drop-in ulcer index from 64.33 ± 8.76 to 32.00 ± 4.47. Similar results were revealed in human gastric mucosal epithelia cells, with cells viability to increase from 27.2 % to 61.6 % post DOFF administration. To analyzed the protect effect of DOFF, we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways. The results showed that DOFF (320 μg/mL) could increase the level of oxidation marker protein (HO-1), apoptosis regulatory protein (Bcl-2) and autophagy marker (LC3β) by 50.84 %, 43.85 %, and 59.21 % compared with ethanol-treated group respectively. Further analyzed of the mitochondrial activity and apoptosis pathway, we found that DOFF appeared to mitigate against ethanol-induced gastric mucosal injury <em>via</em> AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3661-3679"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140464400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01Epub Date: 2024-12-18DOI: 10.26599/FSHW.2023.9250014
Rongxin Wen , Yumeng Sui , Jiaqi Liu , Huiping Wang , Baohua Kong , Ligang Qin , Qian Chen
The effects of the co-inoculation of Debaryomyces hansenii separately with 3 lactic acid bacteria (LAB), Lactobacillus sakei, Lactobacillus plantarum and Lactobacillus curvatus, on the taste and odour profiles of dry sausages were investigated. The co-inoculated sausages showed higher free amino acid and organic acid contents than the non-inoculated control and sausages inoculated with D. hansenii alone. Meanwhile, the sausages inoculated with D. hansenii + L. plantarum, D. hansenii + L. sakei and D. hansenii + L. curvatus had the highest contents of aldehydes, esters and alcohols, respectively. The results of electronic tongue, electronic nose and sensory evaluation demonstrated that compared with the sausage inoculated with D. hansenii, the sour taste and floral odour increased and the fatty odour decreased in the sausage inoculated with D. hansenii + L. sakei; this was more favourable for the development of a desirable flavour in sausages. Moreover, the partial least squares regression analysis indicated that 10 taste and 33 odour compounds were mainly responsible for the differences in the flavour profiles among the sausages. Overall, these findings contributed to a more comprehensive understanding of the formation of sensory characteristics in dry sausages co-inoculated with yeast and LAB.
{"title":"Co-inoculation of Debaryomyces hansenii and lactic acid bacteria: a strategy to improve the taste and odour profiles of dry sausages","authors":"Rongxin Wen , Yumeng Sui , Jiaqi Liu , Huiping Wang , Baohua Kong , Ligang Qin , Qian Chen","doi":"10.26599/FSHW.2023.9250014","DOIUrl":"10.26599/FSHW.2023.9250014","url":null,"abstract":"<div><div>The effects of the co-inoculation of <em>Debaryomyces hansenii</em> separately with 3 lactic acid bacteria (LAB), <em>Lactobacillus sakei</em>, <em>Lactobacillus plantarum</em> and <em>Lactobacillus curvatus</em>, on the taste and odour profiles of dry sausages were investigated. The co-inoculated sausages showed higher free amino acid and organic acid contents than the non-inoculated control and sausages inoculated with <em>D. hansenii</em> alone. Meanwhile, the sausages inoculated with <em>D. hansenii</em> + <em>L. plantarum</em>, <em>D. hansenii</em> + <em>L. sakei</em> and <em>D. hansenii</em> + <em>L. curvatus</em> had the highest contents of aldehydes, esters and alcohols, respectively. The results of electronic tongue, electronic nose and sensory evaluation demonstrated that compared with the sausage inoculated with <em>D. hansenii</em>, the sour taste and floral odour increased and the fatty odour decreased in the sausage inoculated with <em>D. hansenii</em> + <em>L. sakei</em>; this was more favourable for the development of a desirable flavour in sausages. Moreover, the partial least squares regression analysis indicated that 10 taste and 33 odour compounds were mainly responsible for the differences in the flavour profiles among the sausages. Overall, these findings contributed to a more comprehensive understanding of the formation of sensory characteristics in dry sausages co-inoculated with yeast and LAB.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3273-3283"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140464992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01Epub Date: 2024-12-18DOI: 10.26599/FSHW.2023.9250041
Xiankang Fan , Laidi Zhang , Yangying Sun , Changyu Zhou , Qiang Xia , Lihui Du , Zhen Wu , Daodong Pan
In this study, 10 novel anti-inflammatory peptides were identified from duck liver, and their molecular mechanism was demonstrated based on machine learning and molecular docking. Using Sephadex G-15 gel chromatography separation, reversed-phase high-performance liquid chromatography purification, liquid chromatography-tandem mass spectrometry identification, and BIOPEP database comparison, 10 novel anti-inflammatory peptides were initially found. Their splendid angiotensin-converting enzyme (ACE) inhibition and anti-inflammatory properties were confirmed by machine learning. With binding energies less than –20.93 kJ/mol, molecular docking revealed that they could efficiently bind to the active pockets of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), cyclooxygenase 2 (COX-2), and nuclear factor κB (NF-κB) proteins with efficiency, indicating that the compounds can spontaneously form complexes through hydrogen bonding and hydrophobic interactions with the protein binding pockets. In the lipopolysaccharide-induced RAW264.7 cell model, the release of NO, TNF-α, and IL-6 and the mRNA expression of inflammatory factors (TNF-α, IL-6, COX-2, and NF-κB) were significantly inhibited by these peptides. We concluded it might be due to their anti-inflammatory effects by inhibiting the protein phosphorylation of inhibitor of NF-κB (IκBα) in the cytoplasm and preventing the translocation of NF-κB p65 in the cytoplasm to the nucleus, thereby regulating the NF-κB signaling pathway. This study is essential for the screening of anti-inflammatory peptides and the investigation of the mechanism of action.
{"title":"Identification and the molecular mechanism of novel duck liver-derived anti-inflammatory peptides in lipopolysaccharide-induced RAW264.7 cell model","authors":"Xiankang Fan , Laidi Zhang , Yangying Sun , Changyu Zhou , Qiang Xia , Lihui Du , Zhen Wu , Daodong Pan","doi":"10.26599/FSHW.2023.9250041","DOIUrl":"10.26599/FSHW.2023.9250041","url":null,"abstract":"<div><div>In this study, 10 novel anti-inflammatory peptides were identified from duck liver, and their molecular mechanism was demonstrated based on machine learning and molecular docking. Using Sephadex G-15 gel chromatography separation, reversed-phase high-performance liquid chromatography purification, liquid chromatography-tandem mass spectrometry identification, and BIOPEP database comparison, 10 novel anti-inflammatory peptides were initially found. Their splendid angiotensin-converting enzyme (ACE) inhibition and anti-inflammatory properties were confirmed by machine learning. With binding energies less than –20.93 kJ/mol, molecular docking revealed that they could efficiently bind to the active pockets of tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), cyclooxygenase 2 (COX-2), and nuclear factor κB (NF-κB) proteins with efficiency, indicating that the compounds can spontaneously form complexes through hydrogen bonding and hydrophobic interactions with the protein binding pockets. In the lipopolysaccharide-induced RAW264.7 cell model, the release of NO, TNF-α, and IL-6 and the mRNA expression of inflammatory factors (<em>TNF-α</em>, <em>IL-6</em>, <em>COX-2</em>, and <em>NF-κB</em>) were significantly inhibited by these peptides. We concluded it might be due to their anti-inflammatory effects by inhibiting the protein phosphorylation of inhibitor of NF-κB (IκBα) in the cytoplasm and preventing the translocation of NF-κB p65 in the cytoplasm to the nucleus, thereby regulating the NF-κB signaling pathway. This study is essential for the screening of anti-inflammatory peptides and the investigation of the mechanism of action.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3595-3605"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140463838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01Epub Date: 2024-12-18DOI: 10.26599/FSHW.2023.9250028
Hongyu Pan , Qianwang Zheng , Yuan Zou , Guozhao Luo , Mingzhen Tu , Na Wang , Jingru Zhong , Liqiong Guo , Junfang Lin
Ergothioneine is a natural amino acid with powerful antioxidant activity. Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans, and enhance telomerase activity. In present study, the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies. And the alterations in the gut barrier, gut morphology, and other gut physiological indicators of aging flies were evaluated. Here, the results showed that ergothioneine induced little variations in gut microbiota composition in flies at mid-life, and significant alterations in the gut microbiota populations were only observed in flies at late-life. And ergothioneine attenuated age-related gut injuries, including decreasing reactive oxygen species levels in intestinal enterocytes, reduction of intestinal cells death, and preservation of gut integrity in aging flies. Ergothioneine also improved the gut morphology by preserving the structure and shape of cell organelles and inducing the intestinal autophagy. And activation of intestinal autophagy also contributed to the longevity effect mediated by ergothioneine in an unidentified mechanism.
{"title":"Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy","authors":"Hongyu Pan , Qianwang Zheng , Yuan Zou , Guozhao Luo , Mingzhen Tu , Na Wang , Jingru Zhong , Liqiong Guo , Junfang Lin","doi":"10.26599/FSHW.2023.9250028","DOIUrl":"10.26599/FSHW.2023.9250028","url":null,"abstract":"<div><div>Ergothioneine is a natural amino acid with powerful antioxidant activity. Previous studies have demonstrated that ergothioneine can increase the lifespan of <em>Drosophila melanogaster</em> and <em>Caenorhabditis elegans</em>, and enhance telomerase activity. In present study, the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies. And the alterations in the gut barrier, gut morphology, and other gut physiological indicators of aging flies were evaluated. Here, the results showed that ergothioneine induced little variations in gut microbiota composition in flies at mid-life, and significant alterations in the gut microbiota populations were only observed in flies at late-life. And ergothioneine attenuated age-related gut injuries, including decreasing reactive oxygen species levels in intestinal enterocytes, reduction of intestinal cells death, and preservation of gut integrity in aging flies. Ergothioneine also improved the gut morphology by preserving the structure and shape of cell organelles and inducing the intestinal autophagy. And activation of intestinal autophagy also contributed to the longevity effect mediated by ergothioneine in an unidentified mechanism.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3434-3446"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140468082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01Epub Date: 2024-12-18DOI: 10.26599/FSHW.2023.9250015
Nana Zhang , Jianlin Liu , Weiyue Zhang , Xinxin Guo , Shuying Li , Hongtai Zhang , Minjie Wang , Bei Fan , Fengzhong Wang
Soy polysaccharide (SP) has been reported to possess the properties of modulating gut microbiome diversity. Here, we aimed to explore the protective effects of SP against dextran sulphate sodium (DSS)-induced colitis. Pre-treatment with SP at a dosage of 400 mg/kg·day alleviated colitis symptoms, preventing the weight loss and colon shorten. SP suppressed DSS-induced inflammatory response and enhanced M1 to M2 macrophage polarization. Further investigation showed that SP significantly promoted the regeneration of crypt and the expansion of goblet cell production. In addition, bacterial 16S rRNA sequencing analysis showed that SP modulated the composition of fecal microbiota, including selectively increasing Lactobacillus relative abundance. Notably, SP treatment enriched the production of Lactobacillus-derived lactic acid, which was sensed by its specific G-protein-coupled receptor 81 (Gpr81)/Wnt3/β-catenin signaling, and promoted the regeneration of intestinal stem cells. Fecal microbiome transplantation demonstrated that intestinal flora partially contributed to the beneficial effects of SP on preventing against colitis. In conclusion, SP exhibited the protective effects against colitis, which could be partly associated with modulating the composition of gut microbiota and enrichment of lactic acid. This study suggests that SP has potential to be developed as nutritional intervention to prevent colitis.
{"title":"Soy polysaccharide maintains colonic homeostasis to protect from dextran sulphate sodium-induced colitis by modulating gut microbiota and intestinal epithelial regeneration","authors":"Nana Zhang , Jianlin Liu , Weiyue Zhang , Xinxin Guo , Shuying Li , Hongtai Zhang , Minjie Wang , Bei Fan , Fengzhong Wang","doi":"10.26599/FSHW.2023.9250015","DOIUrl":"10.26599/FSHW.2023.9250015","url":null,"abstract":"<div><div>Soy polysaccharide (SP) has been reported to possess the properties of modulating gut microbiome diversity. Here, we aimed to explore the protective effects of SP against dextran sulphate sodium (DSS)-induced colitis. Pre-treatment with SP at a dosage of 400 mg/kg·day alleviated colitis symptoms, preventing the weight loss and colon shorten. SP suppressed DSS-induced inflammatory response and enhanced M1 to M2 macrophage polarization. Further investigation showed that SP significantly promoted the regeneration of crypt and the expansion of goblet cell production. In addition, bacterial 16S rRNA sequencing analysis showed that SP modulated the composition of fecal microbiota, including selectively increasing <em>Lactobacillus</em> relative abundance. Notably, SP treatment enriched the production of <em>Lactobacillus</em>-derived lactic acid, which was sensed by its specific G-protein-coupled receptor 81 (Gpr81)/Wnt3/<em>β</em>-catenin signaling, and promoted the regeneration of intestinal stem cells. Fecal microbiome transplantation demonstrated that intestinal flora partially contributed to the beneficial effects of SP on preventing against colitis. In conclusion, SP exhibited the protective effects against colitis, which could be partly associated with modulating the composition of gut microbiota and enrichment of lactic acid. This study suggests that SP has potential to be developed as nutritional intervention to prevent colitis.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3284-3300"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140467863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01Epub Date: 2024-12-18DOI: 10.26599/FSHW.2023.9250006
Minyu Wu , Changfeng Hu , Lirong Shen
Numerous studies currently compare the lipid metabolism in patients with cardiovascular disease (CVD) and healthy individuals to identify lipid markers for predicting CVD. In this study, multidimensional mass spectrometry-based shotgun lipidomics was used to examine the serum lipidomics of participants in a clinical randomized controlled feeding trial undergoing olive oil (OO), camellia seed oil (CSO), and soybean oil (SO) dietary interventions. 189 lipid molecules are identified, including 14 species of phosphatidylinositol, 45 species of ethanolamine glycerols (PE), 47 species of choline glycerophospholipids (PC), 39 species of triacylglycerols (TAG), 18 species of lysophosphatidylcholine, and 26 species of sphingomyelin. After screening, 10 lipid markers are found, among which 18:2 fatty acid (FA), 16:1 FA, C54:4/C55:11, C54:3/C55:10, and C52:3/C53:10 in TAG pool, p18:0/20:0 and a18:0/18:1 in PC pool, and p18:1/20:4 in PE pool have differential regulation in the SO group compared to OO and CSO. The d16:0/18:1 in PC pool and C52:2/C53:9 in TAG pool are differentially regulated by OO and CSO. The C52:2/C53:9 in TAG pool has a significant negative correlation with aspartate aminotransferase (r = -0.363, P = 0.048) and high-density lipoprotein cholesterol (r = -0.519, P < 0.01). This study provides a reference for researching the effect of dietary fat on blood lipid metabolism.
目前，许多研究比较了心血管疾病（CVD）患者和健康个体的脂质代谢，以确定预测CVD的脂质标志物。在这项研究中，基于多维质谱的霰弹枪脂质组学被用于检测一项临床随机对照喂养试验参与者的血清脂质组学，这些参与者接受橄榄油（OO）、茶籽油（CSO）和大豆油（SO）的饮食干预。共鉴定出189种脂类分子，其中磷脂酰肌醇14种，乙醇胺甘油（PE） 45种，胆碱甘油磷脂（PC） 47种，三酰基甘油（TAG） 39种，溶血磷脂18种，鞘磷脂26种。经筛选，发现10个脂质标志物，其中TAG组18:2脂肪酸（FA）、16:1脂肪酸、C54:4/C55:11、C54:3/C55:10、C52:3/C53:10、PC组p18:0/20:0、a18:0/18:1、PE组p18:1/20:4在SO组与OO、CSO组有差异调节。PC池中的d16:0/18:1和TAG池中的C52:2/C53:9受到OO和CSO的不同调节。TAG池C52:2/C53:9与天冬氨酸转氨酶（r = -0.363, P = 0.048）、高密度脂蛋白胆固醇(r = -0.519, P <；0.01)。本研究为研究膳食脂肪对血脂代谢的影响提供了参考。
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Pub Date : 2024-11-01Epub Date: 2024-12-18DOI: 10.26599/FSHW.2023.9250032
Yue Liu , Ping Ren , Hailong Li , Yingying Liu , Yiting Yang , Wanting Wang , Jiaming Zhou , Ling Su , Lili Guan , Hongxia Ma
This study aimed to explore the protective effect and potential mechanism of Nostoc commune Vauch. polysaccharide (NCVP) on lead (Pb)-poisoning mice. NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses and modulated key indicators of antioxidant capacity. Moreover, the down-regulation of critical proteins of the Nrf2 pathway induced by Pb could be reversed after NCVP intervention. In addition, NCVP maintained the diversity of gut bacteriobiota and restored the relative abundance of f_Prevotellaceae, g_Alloprevotella, and f_ Eubacterium_coprostanoligenes_group reduced by Pb. Also, NCVP regulated the diversity and abundance of gut mycobiota affected by Pb. Specifically, Pb decreased the proportion of pathogenic species (g_Fusarium, p_Basidiomycota, g_Alternaria, g_Aspergillus, and g_Candida) while NCVP increased the abundance of probiotics species (g_Kazachstania and p_Ascomycota). Furthermore, the metabolomic analysis found that NCVP significantly altered a range of microbial metabolites, including porphobilinogen, cromakalim, salidroside, and trichostatin A, which has significant associations with specific gut bacteriobiota or mycobiota. These altered metabolites are involved in primary bile acid biosynthesis, metabolism of xenobiotics by cytochrome P450, lysine degradation, and other metabolic pathways. Overall, our findings indicate that NCVP might be an excellent natural product for eliminating Pb-induced hepatorenal toxicity, possibly by regulating gut bacteriome, mycobiome and metabolome.
{"title":"Nostoc commune Vauch. polysaccharide alleviates Pb-induced hepatorenal toxicity by changing gut bacteriome, mycobiome, and metabolome","authors":"Yue Liu , Ping Ren , Hailong Li , Yingying Liu , Yiting Yang , Wanting Wang , Jiaming Zhou , Ling Su , Lili Guan , Hongxia Ma","doi":"10.26599/FSHW.2023.9250032","DOIUrl":"10.26599/FSHW.2023.9250032","url":null,"abstract":"<div><div>This study aimed to explore the protective effect and potential mechanism of <em>Nostoc commune</em> Vauch. polysaccharide (NCVP) on lead (Pb)-poisoning mice. NCVP improved Pb-induced hepatorenal toxicity and inflammatory responses and modulated key indicators of antioxidant capacity. Moreover, the down-regulation of critical proteins of the Nrf2 pathway induced by Pb could be reversed after NCVP intervention. In addition, NCVP maintained the diversity of gut bacteriobiota and restored the relative abundance of f_Prevotellaceae, g_<em>Alloprevotella</em>, and f_ <em>Eubacterium_coprostanoligenes</em>_group reduced by Pb. Also, NCVP regulated the diversity and abundance of gut mycobiota affected by Pb. Specifically, Pb decreased the proportion of pathogenic species (g_<em>Fusarium</em>, p_Basidiomycota, g_<em>Alternaria</em>, g_<em>Aspergillus</em>, and g_<em>Candida</em>) while NCVP increased the abundance of probiotics species (g_<em>Kazachstania</em> and p_Ascomycota). Furthermore, the metabolomic analysis found that NCVP significantly altered a range of microbial metabolites, including porphobilinogen, cromakalim, salidroside, and trichostatin A, which has significant associations with specific gut bacteriobiota or mycobiota. These altered metabolites are involved in primary bile acid biosynthesis, metabolism of xenobiotics by cytochrome P450, lysine degradation, and other metabolic pathways. Overall, our findings indicate that NCVP might be an excellent natural product for eliminating Pb-induced hepatorenal toxicity, possibly by regulating gut bacteriome, mycobiome and metabolome.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3482-3500"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140466172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-01Epub Date: 2024-12-18DOI: 10.26599/FSHW.2023.9250008
Yingshuang Lu , Renbing Qin , Jin Wang , Fengjuan Xiang , Yan Zhang , Bowei Zhang , Dancai Fan , Huan Lü , Xuemeng Ji , Shujun Wang , Shuo Wang
It has been widely accepted that resistant starch (RS) provides numerous health benefits for human. In this research, we aimed at evaluating the performance of novel starch-lipid complexes, RS5, in comparison with RS2 on physical features, glucolipids metabolism, inflammation, and gut microbiota profiles of type 2 diabetes mellitus (T2DM) rats. The T2DM model was established by streptozotocin injection to the high-fat-sugar fed rats. According to a serial of biochemical analyses, we found that RS5 diets were strongly correlated with enhanced homeostatic model assessment for insulin secretion (HOMA-IS), high-density lipoprotein cholesterol (HDL-C), adiponectin (ADP), insulin action index (IAI), glucagon-like peptide-1 (GLP1), and short-chain fatty acids (SCFAs) in T2DM rats whilst negatively associated with the low-density lipoprotein (LDL-C) and inflammatory cytokines, showing the capabilities to ameliorate T2DM symptoms by regulation of glucolipid metabolism, gut metabolites, and inflammation. On the other hand, RS2-enriched supplementations were influential in the mediation of insulin secretion to improve glucose metabolism. The increasing evidence collected herein suggested that intestinal microbiota could mediate glucolipids metabolism and alleviate inflammation after certain microflora nourished by RS. In addition, RS intake made an impact on phosphoinositide 3-kinase/protein kinase B signaling pathway that might contribute to the improvement of glucose metabolism, insulin resistance, and inflammatory responses.
{"title":"The impact of the novel starch-lipid complexes on the glucolipids metabolism, inflammation, and gut dysbiosis of type 2 diabetes mellitus rats","authors":"Yingshuang Lu , Renbing Qin , Jin Wang , Fengjuan Xiang , Yan Zhang , Bowei Zhang , Dancai Fan , Huan Lü , Xuemeng Ji , Shujun Wang , Shuo Wang","doi":"10.26599/FSHW.2023.9250008","DOIUrl":"10.26599/FSHW.2023.9250008","url":null,"abstract":"<div><div>It has been widely accepted that resistant starch (RS) provides numerous health benefits for human. In this research, we aimed at evaluating the performance of novel starch-lipid complexes, RS5, in comparison with RS2 on physical features, glucolipids metabolism, inflammation, and gut microbiota profiles of type 2 diabetes mellitus (T2DM) rats. The T2DM model was established by streptozotocin injection to the high-fat-sugar fed rats. According to a serial of biochemical analyses, we found that RS5 diets were strongly correlated with enhanced homeostatic model assessment for insulin secretion (HOMA-IS), high-density lipoprotein cholesterol (HDL-C), adiponectin (ADP), insulin action index (IAI), glucagon-like peptide-1 (GLP1), and short-chain fatty acids (SCFAs) in T2DM rats whilst negatively associated with the low-density lipoprotein (LDL-C) and inflammatory cytokines, showing the capabilities to ameliorate T2DM symptoms by regulation of glucolipid metabolism, gut metabolites, and inflammation. On the other hand, RS2-enriched supplementations were influential in the mediation of insulin secretion to improve glucose metabolism. The increasing evidence collected herein suggested that intestinal microbiota could mediate glucolipids metabolism and alleviate inflammation after certain microflora nourished by RS. In addition, RS intake made an impact on phosphoinositide 3-kinase/protein kinase B signaling pathway that might contribute to the improvement of glucose metabolism, insulin resistance, and inflammatory responses.</div></div>","PeriodicalId":12406,"journal":{"name":"Food Science and Human Wellness","volume":"13 6","pages":"Pages 3210-3223"},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140467164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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