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Effects of shrub expansion on methane emissions from temperate wetlands and their regulatory mechanisms 温带湿地灌木扩张对甲烷排放的影响及其调控机制
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-21 DOI: 10.1016/j.geoderma.2026.117693
Shenzheng Wang , Xin Sui , Haixiu Zhong , Xiaoyu Fu , Rongtao Zhang , Yingnan Liu
<div><div>Climate warming and human activities have led to widespread expansion of shrubs in many wetlands, altering the distribution patterns of native vegetation and disrupting C cycling. Although the effects of shrub expansion on soil microbial communities and methane (CH<sub>4</sub>) emissions have been extensively studied, the specific microbial-mediated pathways involved in methane cycling remain unexplored. The static chamber method was used to investigate the characteristics of CH<sub>4</sub> emission flux changes under different levels of shrub expansion. Additionally, metagenomics technology was employed to assess the effects of different shrub expansion levels on soil microbial community composition, function, and diversity (bacteria and fungi), as well as the methane metabolic pathways mediated by these communities. Shrub expansion in wetlands was categorized into four classes based on shrub coverage. We found that methane flux decreased significantly with increasing shrub expansion, with cumulative emissions under extensive expansion conditions being only 28 % of those under no expansion conditions. The peak emissions on August 15 under no expansion conditions were 2–3 times higher than those in shrub-expanded plots. The partial least squares path model (PLS-PM, GOF = 0.731) indicated that shrub expansion enhanced soil physicochemical properties (β = 0.865), which inhibited methanogenic genes (β = −0.617) and activated methane oxidation pathways (total effect β = 0.728). Methane-oxidizing genes contributed the most to CH<sub>4</sub> reduction, accounting for 72.4 % of the pathway effect. This was primarily manifested as inhibition of key genes involved in the acetate pathway for methane production (comB and hdrA) and upregulation of methane-oxidizing-related genes (mmoB and DAK). Shrub expansion significantly increased soil ammonium nitrogen content while reducing soil moisture content. Although bacterial α diversity remained unchanged, the fungal Chao1 index significantly increased. Additionally, MI and HI treatments significantly altered bacterial community structure, while fungal communities remained relatively stable. The relative abundance of Verrucomicrobia initially increased with shrub expansion but decreased at higher levels, while Ascomycota, Basidiomycota, and Mucoromycota showed significant increases. The study suggests that the reduction in CH<sub>4</sub> emissions caused by shrub expansion is primarily regulated by a synergistic pathway involving the combined effects of soil physicochemical properties and oxidative microbial genes. Furthermore, bacterial communities are more sensitive to shrub expansion than fungal communities. These results highlight the complex interactions between aboveground vegetation dynamics, soil microbial communities, greenhouse gas fluxes, and environmental factors. However, this CH<sub>4</sub> reduction likely reflects hydrological degradation and wetland desiccation, which may increase CO<su
气候变暖和人类活动导致许多湿地灌木大面积扩张,改变了原生植被的分布格局,破坏了碳循环。尽管灌木扩张对土壤微生物群落和甲烷(CH4)排放的影响已被广泛研究,但参与甲烷循环的特定微生物介导途径仍未被探索。采用静室法研究了不同灌木扩张水平下CH4排放通量的变化特征。此外,利用宏基因组学技术评估了不同灌木扩张水平对土壤微生物群落组成、功能和多样性(细菌和真菌)的影响,以及这些群落介导的甲烷代谢途径。根据灌木盖度将湿地灌木扩张分为4类。研究发现,随着灌木扩张的增加,甲烷通量显著降低,粗放扩张条件下的累积排放量仅为无扩张条件下的28%。8月15日无扩展条件下的峰值排放量是灌木扩展样地的2 ~ 3倍。偏最小二乘路径模型(PLS-PM, GOF = 0.731)表明,灌木扩张增强了土壤理化性质(β = 0.865),抑制了产甲烷基因(β = - 0.617),激活了甲烷氧化途径(总效应β = 0.728)。甲烷氧化基因对CH4还原的贡献最大,占途径效应的72.4%。这主要表现为参与甲烷生成醋酸途径的关键基因(comB和hdrA)的抑制和甲烷氧化相关基因(mmoB和DAK)的上调。灌木扩张显著提高了土壤铵态氮含量,降低了土壤水分含量。虽然细菌α多样性保持不变,但真菌Chao1指数显著升高。此外,MI和HI处理显著改变了细菌群落结构,而真菌群落保持相对稳定。Verrucomicrobia的相对丰度最初随着灌木的扩大而增加,但随着灌木的扩大而降低,而子囊菌门、担子菌门和毛霉门的相对丰度则显著增加。研究表明,灌木扩张对CH4排放的减少主要受土壤理化性质和氧化微生物基因共同作用的协同调控。此外,细菌群落比真菌群落对灌木扩张更敏感。这些结果强调了地上植被动态、土壤微生物群落、温室气体通量和环境因素之间复杂的相互作用。然而,这种CH4的减少可能反映了水文退化和湿地干燥,这可能会增加氧化泥炭的二氧化碳排放,损害长期的碳固存,强调了防止灌木入侵湿地保护的必要性。
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引用次数: 0
Nitrogen fertilization induces greater loss of base cations and accumulation of exchangeable acids in acidic soils than in neutral soils 与中性土壤相比,施用氮肥导致酸性土壤中碱性阳离子的损失和交换性酸的积累更大
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-12 DOI: 10.1016/j.geoderma.2025.117669
Shijie He , Beilei Wei , Hao Guo , Huarong Lin , Ruixuan Zhu , Xiaoqi Zhang , Shunting He , Yongfeng Sun , Shengsen Zhou , Andong Cai , Ziting Wang , Zhigang Huang
Agricultural soil acidification affects 40–70% of croplands worldwide and is intensifying with increasing nitrogen fertilization. Although nitrogen fertilizer-induced acidification is well-documented, the quantitative relationships and thresholds between base cations and exchangeable acids across different soil pH levels remain poorly understood. This study conducted a meta-analysis of 2,348 field trials from 157 sites worldwide, quantifying the dynamics of base cations (K+, Ca2+, Mg2+) and exchangeable acids in acidic and neutral soils under long-term nitrogen fertilization. It aims to: (1) quantify nitrogen effects on base cations and exchangeable acids; (2) evaluate the relationship between base cation loss and pH decline in soils with different pH values; and (3) explore how organic matter alleviates exchangeable acids increase. The results showed that under nitrogen fertilization, the exchangeable K+, Ca2+, and Mg2+ decreased significantly in acidic soils by 20.3%, 48.6%, and 43.3%, respectively, but showed relative lower decreases in neutral soils by 17.9%, 14.5%, and 6.3%, respectively. In addition, the exchangeable acid content in acidic soils increased significantly by 116.5%, which was much higher than that in neutral soils (35.7%). Subgroup analyses revealed that the accumulation of exchangeable acids could be effectively inhibited when the soil organic matter content exceeded 25.0 g/kg; moreover, the addition of calcium was of great significance for the retention of soil organic matter. This study emphasizes that acidic soils are more sensitive to nitrogen fertilization than neutral soils and more susceptible to soil acidification. These findings are crucial for understanding the acidification threshold of nitrogen fertilizer application, accumulative effects of fertilization time, and dynamic equilibrium between base cations and exchangeable acids in soils with different pH values. They also provide scientific evidence for quantifying the threshold of organic matter content for acidification relief and mitigating soil acidification caused by nitrogen fertilization.
农业土壤酸化影响着全世界40-70%的农田,并且随着施氮量的增加而加剧。虽然氮肥引起的酸化有充分的文献记载,但碱阳离子和交换性酸在不同土壤pH水平上的定量关系和阈值仍然知之甚少。本研究对来自全球157个站点的2348个田间试验进行了荟萃分析,量化了长期施氮条件下酸性和中性土壤中碱性阳离子(K+、Ca2+、Mg2+)和交换性酸的动态。其目的是:(1)量化氮对碱性阳离子和交换性酸的影响;(2)评价不同pH值土壤碱阳离子流失与pH下降的关系;(3)探讨有机质如何缓解交换性酸的增加。结果表明:施氮处理下,酸性土壤的可交换性K+、Ca2+和Mg2+分别显著降低20.3%、48.6%和43.3%,中性土壤的可交换性K+、Ca2+和Mg2+降低幅度较小,分别为17.9%、14.5%和6.3%。酸性土壤的交换性酸含量显著增加116.5%,远高于中性土壤的35.7%。亚群分析表明,当土壤有机质含量超过25.0 g/kg时,可有效抑制交换性酸的积累;此外,钙的添加对土壤有机质的保留具有重要意义。本研究强调酸性土壤对氮肥施用比中性土壤更敏感,更容易发生土壤酸化。这些发现对于理解不同pH值土壤中施用氮肥的酸化阈值、施肥时间的累积效应以及碱阳离子与交换性酸之间的动态平衡具有重要意义。为定量确定土壤有机质含量阈值,缓解土壤酸化、缓解氮肥土壤酸化提供科学依据。
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引用次数: 0
Horizontal gene transfer between bacteriophages and their hosts is a key factor in the bloom of antibiotic resistance genes in Metaphire californica 噬菌体与寄主之间的水平基因转移是加利福尼亚中期抗生素抗性基因开花的关键因素
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-19 DOI: 10.1016/j.geoderma.2026.117676
Hong-Xia Cui , Qing-Fang Bi , Jun Ye , Yu-Chen Li , Hu Liao , Jian-Qiang Su
Earthworm guts are a reservoir for antibiotic resistance genes (ARGs). However, the role of horizontal gene transfer (HGT) between bacteriophages and their hosts in amplifying ARG proliferation within this niche remains limited. To address this knowledge gap, we analyzed paired metagenomes from Metaphire californica guts and surrounding soil collected from farmland in Zhejiang, China. Earthworm guts harbored greater ARG diversity than soil (323 vs 303 subtypes), with significantly elevated abundances of macrolide-lincosamide-streptogramin (MLS), novobiocin, rifamycin, and tetracycline resistance determinants. Consistent with this, viral diversity was also higher in the earthworm gut comparing with soil. We recovered 67 microbial metagenome-assembled genomes (MAGs) and 5,703 viral operational taxonomic units (vOTUs), which enabled us to evaluate the contribution of viruses to ARG dissemination across different environments. Notably, virus-encoded ARGs were distributed across 43 Resfams families (RFs), with 32 detected in earthworm gut and only 23 in soil. Crucially, 1,568 potential HGT events between vOTUs and MAGs were identified, with a significantly higher frequency observed in the earthworm gut (1,110 events) compared to soil (458 events). Interestingly, the potential HGT regions against the Resfams database revealed that 32 gut-associated HGT events involved 11 RFs, whereas only 12 events associated with 5 RFs were detected in soil. These findings reveal the earthworm gut’s role in accelerating antibiotic resistance proliferation within agricultural ecosystems, highlighting the interconnected health risks emphasized by the One Health framework.
蚯蚓肠道是抗生素耐药基因(ARGs)的储存库。然而,噬菌体与其宿主之间的水平基因转移(HGT)在该生态位内扩增ARG增殖中的作用仍然有限。为了解决这一知识空白,我们分析了从中国浙江农田收集的加利福尼亚野鱼内脏和周围土壤的配对宏基因组。蚯蚓肠道的ARG多样性高于土壤(323个亚型对303个亚型),大环内酯- lincosamde -streptogramin (MLS)、新生物素、利福霉素和四环素耐药决定因子的丰度显著升高。与此一致的是,蚯蚓肠道中的病毒多样性也高于土壤。我们恢复了67个微生物宏基因组组装基因组(MAGs)和5703个病毒操作分类单位(vOTUs),这使我们能够评估病毒在不同环境中对ARG传播的贡献。值得注意的是,病毒编码的ARGs分布在43个refam家族(rf)中,其中32个在蚯蚓肠道中检测到,只有23个在土壤中检测到。关键是,在vOTUs和MAGs之间确定了1568个潜在的HGT事件,在蚯蚓肠道中观察到的频率(1110个事件)明显高于土壤(458个事件)。有趣的是,针对Resfams数据库的潜在HGT区域显示,32个与肠道相关的HGT事件涉及11个rf,而在土壤中仅检测到12个与5个rf相关的事件。这些发现揭示了蚯蚓肠道在加速农业生态系统内抗生素耐药性扩散方面的作用,突出了“同一个健康”框架所强调的相互关联的健康风险。
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引用次数: 0
Aridity dependency of soil plant- and microbial-derived carbon in mongolia plateau in northern China 蒙古高原土壤植物碳和微生物碳的干旱依赖性
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-26 DOI: 10.1016/j.geoderma.2026.117706
Shaoyu Li, Lishan Yang, Feng Zhang, Jiahua Zheng, Bin Zhang, Guodong Han, Mengli Zhao
Soil plant-derived carbon (PDC) and microbial-derived carbon (MDC) represent the two primary sources of soil organic carbon (SOC). The relative proportions of these carbon (C) sources shape SOC composition, accumulation, stability, and turnover. Dryland ecosystems, while serving as critical C reservoirs, are particularly vulnerable to climate change. However, the influence of aridity on PDC and MDC sequestration remains understudied at the regional scale, which limits our ability to understand and predict soil C dynamics in drylands under the context of global warming. To address this knowledge gap, we measured PDC, MDC, and associated biotic and abiotic variables at 90 sampling sites along a ∼ 3000 km transect across the Mongolian Plateau. We examined the biogeographical distribution patterns of PDC and MDC along a natural aridity gradient. Our findings revealed that both PDC and MDC declined concurrently in response to increasing aridity. Based on the contributions of PDC and MDC to SOC, we identified the shifting point marking the transition in the dominance of different pathways of the microbial C pump. This shifting point was further validated using microbial C use efficiency (CUE) and soil extracellular enzyme activity. In subhumid-semiarid region (aridity = 0.37), the contribution of PDC to SOC exceeds that of MDC, coupled with higher extracellular enzyme activity, indicating the dominance of ex vivo modification. Here, PDC formation is primarily driven by the microbial fragmentation of abundant plant litter. High precipitation and nutrient availability in these areas further support the conversion of microbial biomass C into MDC. In contrast, in semiarid region (aridity = 0.78), the contribution of PDC to SOC is lower than that of MDC, coupled with higher microbial CUE, indicating the dominance of in vivo turnover. MDC accumulation is promoted by physical protection mechanisms, such as increased clay and silt content, while PDC formation is constrained by limited root C inputs. Our findings provide new insights into the mechanisms of SOC sequestration in drylands and emphasize the need to consider both PDC and MDC in strategies aimed at preserving the terrestrial C sink under current and future climate change.
土壤植物源碳(PDC)和微生物源碳(MDC)是土壤有机碳(SOC)的两大主要来源。这些碳(C)源的相对比例决定了有机碳的组成、积累、稳定和周转。旱地生态系统虽然是关键的碳库,但特别容易受到气候变化的影响。然而,在区域尺度上,干旱对PDC和MDC固存的影响尚未得到充分研究,这限制了我们理解和预测全球变暖背景下旱地土壤C动态的能力。为了解决这一知识差距,我们在蒙古高原沿3000公里横断面的90个采样点测量了PDC、MDC以及相关的生物和非生物变量。我们研究了PDC和MDC沿自然干旱梯度的生物地理分布格局。我们的研究结果表明,PDC和MDC同时下降,以应对干旱的增加。基于PDC和MDC对有机碳的贡献,我们确定了标志着微生物碳泵不同途径优势转变的转移点。利用微生物C利用效率(CUE)和土壤胞外酶活性进一步验证了这一转移点。在亚湿润半干旱区(干旱度= 0.37),PDC对SOC的贡献超过MDC,且胞外酶活性较高,表明体外修饰占主导地位。在这里,PDC的形成主要是由丰富的植物凋落物的微生物破碎化驱动的。这些地区的高降水量和养分有效性进一步支持微生物生物量C向MDC的转化。而在半干旱区(干旱度= 0.78),PDC对SOC的贡献低于MDC,且微生物CUE较高,表明体内转换占主导地位。MDC的积累受到物理保护机制的促进,例如粘土和粉砂含量的增加,而PDC的形成受到有限的根C输入的限制。我们的研究结果为旱地有机碳封存机制提供了新的见解,并强调在当前和未来气候变化下,在保护陆地碳汇的策略中需要考虑PDC和MDC。
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引用次数: 0
Earthworms facilitate soil mineral associated organic matter formation but increase priming effect depending on litter addition and soil texture 蚯蚓促进土壤矿物相关有机质的形成,但根据凋落物添加量和土壤质地的不同,蚯蚓会增加土壤的启动效应
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-22 DOI: 10.1016/j.geoderma.2026.117690
Hongliang Li, Charles R. Warren, Andrew J. Holmes, Claudia Keitel, Feike A. Dijkstra
Earthworms affect soil organic carbon (SOC) decomposition and C stabilization into mineral associated organic matter (MAOM) following fresh organic matter input. However, it remains untested how these earthworm-induced C dynamics vary with the rate of fresh organic matter input and soil texture and how they are associated with soil microbial C use efficiency (CUE). Herein, we conducted a 48-day incubation to investigate the impact of earthworms on soil C dynamics following litter input, as well as the relationships of C dynamics with microbial CUE. The experimental set-up consisted of three factors including earthworms (with and without), 13C-labeled grass litter input rate (0, 1 and 6 g C kg−1 soil) and soil texture (grassland soils with either clay or sand addition). Earthworms increased SOC decomposition without litter input by 9 % − 13 %, while amplifying the priming effect (PE) in soil with clay and sand addition at the highest litter addition by 24 % − 139 %, but decreasing the PE in soil with sand and low litter addition by 32 %. In soil with sand addition, earthworms increased MAOM formation efficiency from litter (fraction of added litter C stabilized in MAOM) by 17 % − 23 %, and the litter C sequestration quotient (litter-derived C in MAOM divided by the sum of litter derived C in MAOM and respiration) by 10 % − 27 %. However, earthworm-induced changes in SOC decomposition, PE and MAOM formation were not associated with earthworm-induced changes in microbial CUE. In conclusion, earthworms can facilitate SOC accrual more in soils with sand addition through disproportional amplification of SOC stabilization compared with SOC loss through decomposition. The influence of earthworms on SOC accrual is more likely driven by physicochemical protection of SOC rather than by changes in microbial metabolism.
蚯蚓影响新鲜有机质输入后土壤有机碳(SOC)分解和碳稳定为矿物伴生有机质(MAOM)。然而,这些蚯蚓诱导的碳动态如何随新鲜有机质输入速率和土壤质地而变化,以及它们如何与土壤微生物碳利用效率(CUE)相关,仍未得到验证。本研究通过48天的培养,研究了蚯蚓对凋落物输入后土壤C动态的影响,以及C动态与微生物CUE的关系。实验设置包括三个因素,包括蚯蚓(有和没有),13c标记的草凋落物输入率(0,1和6 g C kg - 1土壤)和土壤质地(添加粘土或沙子的草地土壤)。蚯蚓使无凋落物输入的土壤有机碳分解提高了9% ~ 13%,而在凋落物添加量最大的土壤中,添加粘土和沙子的土壤的启动效应(PE)提高了24% ~ 139%,而在添加沙子和低凋落物的土壤中,PE降低了32%。在添加沙子的土壤中,蚯蚓使凋落物中MAOM的形成效率(添加的凋落物C稳定在MAOM中的比例)提高了17% ~ 23%,凋落物C的固存商(MAOM中凋落物衍生的C除以MAOM中凋落物衍生的C和呼吸的总和)提高了10% ~ 27%。然而,蚯蚓诱导的SOC分解、PE和MAOM形成的变化与蚯蚓诱导的微生物CUE变化无关。综上所述,蚯蚓通过对土壤有机碳稳定化的不成比例放大,促进了土壤有机碳的积累,而不是通过分解导致的有机碳损失。蚯蚓对有机碳积累的影响更可能是由有机碳的物理化学保护驱动的,而不是由微生物代谢的变化驱动的。
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引用次数: 0
100-m and 1000-m regular grid observations reveal vegetation-controlled ground surface thermal differentiation in the alpine permafrost of the Headwater Area of the Yellow River 100 m和1000 m规则格网观测揭示了黄河源区高寒多年冻土植被控制的地表热分异
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-29 DOI: 10.1016/j.geoderma.2026.117696
Jia Liu , Dongliang Luo , Shizhen Li , Chenyang Peng , Fangfang Chen , Kefei Du , Qingbai Wu , Huijun Jin , Yajuan Zao , Qi Shen
Ground surface temperature (GST) serves as the upper thermal boundary condition governing the thermal regime of permafrost, yet its fine-scale spatial heterogeneity associated with complex surface characteristics remains a critical uncertainty, largely due to a scarcity of high-density in-situ observations. To bridge this gap, we established a multi-scale systematic monitoring network in June-July 2023 at a representative alpine meadow site in the Headwater Area of the Yellow River, northeastern Qinghai-Xizang Plateau. The network comprises two regular grids with extents of 100 m × 100 m and 1000 m × 1000 m (121 nodes each), capturing hourly GST dynamics. By integrating thermal data with high-resolution (0.1 m) unmanned aerial vehicle (UAV)-derived fractional vegetation cover (FVC), we identified a non-linear vegetation forcing mechanism. A cooling optimum was observed at medium FVC (0.5–0.75), yielding the lowest MAGST (−0.56 ± 0.06 °C and −0.71 ± 0.12 °C for the 100-m and 1000-m grids, respectively) by effectively offsetting summer radiative heating against winter insulation. Conversely, low FVC grid points showed amplified diurnal variability (up to 6.09 °C). Spatial analysis revealed scale-dependent thermal regimes: the fine-scale 100-m grid highlighted localized heat accumulation linked to micro-scale surface heterogeneity, while the 1000-m grid showed seasonal structural instability, where coherent spatial patterns disintegrated during winter. These findings provide critical, scale-dependent constraints for calibrating process-based permafrost models.
地表温度(GST)是控制多年冻土热状态的上热边界条件,但其与复杂地表特征相关的精细尺度空间异质性仍然是一个关键的不确定性,这主要是由于缺乏高密度的原位观测。为了弥补这一空白,我们于2023年6 - 7月在青藏高原东北部黄河源区具有代表性的高寒草甸样地建立了多尺度系统监测网络。该网络包括两个规则网格,范围为100米× 100米和1000米× 1000米(每个节点121个),捕捉每小时的GST动态。通过将热数据与高分辨率(0.1 m)无人机(UAV)衍生的植被覆盖度(FVC)相结合,我们确定了非线性植被强迫机制。在中等植被覆盖度(0.5-0.75)下,通过有效地抵消夏季辐射加热和冬季保温,可以产生最低的MAGST(100米和1000米网格分别为- 0.56±0.06°C和- 0.71±0.12°C)。相反,低植被覆盖度网格点显示出放大的日变化(高达6.09°C)。空间分析揭示了尺度相关的热状态:100米网格强调了与微尺度表面异质性相关的局部热积累,而1000米网格显示了季节性结构不稳定性,其中连贯的空间格局在冬季解体。这些发现为校准基于过程的永久冻土模型提供了关键的、依赖于尺度的约束。
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引用次数: 0
SoilNet: A multimodal multitask model for hierarchical classification of soil horizons 土壤网:一个多模态多任务土壤层的分层分类模型
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-21 DOI: 10.1016/j.geoderma.2026.117684
Vipin Singh , Teodor Chiaburu , Einar Eberhardt , Stefan Broda , Joey Prüssing , Frank Haußer , Felix Bießmann
Recent advances in artificial intelligence (AI), in particular foundation models, have improved the state of the art in many application domains including geosciences. Some specific problems, however, could not benefit from this progress yet. Soil horizon classification, for instance, remains challenging because of its multimodal and multitask characteristics and a complex hierarchically structured label taxonomy. Accurate classification of soil horizons is crucial for monitoring soil condition, which directly impacts agricultural productivity, food security, ecosystem stability and climate resilience. In this work, we propose SoilNet - a multimodal multitask model to tackle this problem through a structured modularized pipeline. In contrast to omnipurpose AI foundation models, our approach is designed to be inherently transparent by following the task structure human experts developed for solving this challenging annotation task. The proposed approach integrates image data and geotemporal metadata to first predict depth markers, segmenting the soil profile into horizon candidates. Each segment is characterized by a set of horizon-specific morphological features. Finally, horizon labels are predicted based on the multimodal concatenated feature vector, leveraging a graph-based label representation to account for the complex hierarchical relationships among soil horizons. Our method is designed to address complex hierarchical classification, where the number of possible labels is very large, imbalanced and non-trivially structured. We demonstrate the effectiveness of our approach on a real-world soil profile dataset and a comprehensive user study with domain experts. Our empirical evaluations demonstrate that SoilNet reliably predicts soil horizons that are plausible and accurate. User study results indicate that SoilNet achieves predictive performance on par with or better than that of human experts in soil horizon classification. All code and experiments can be found in our repository: https://github.com/calgo-lab/BGR/.
人工智能(AI)的最新进展,特别是基础模型,提高了包括地球科学在内的许多应用领域的技术水平。然而,一些具体问题还不能从这一进展中受益。例如,土壤水平分类仍然具有挑战性,因为它具有多模式和多任务特征,并且具有复杂的分层结构标签分类法。土壤层位的准确分类对土壤状况监测至关重要,直接影响农业生产力、粮食安全、生态系统稳定性和气候适应能力。在这项工作中,我们提出了一个多模态多任务模型SoilNet,通过结构化的模块化管道来解决这个问题。与通用AI基础模型相比,我们的方法被设计为遵循人类专家为解决这一具有挑战性的注释任务而开发的任务结构,从而具有固有的透明度。该方法将图像数据和时间元数据相结合,首先预测深度标记,然后将土壤剖面分割为候选层位。每个片段都具有一组特定于水平的形态特征。最后,基于多模态连接特征向量预测水平标签,利用基于图的标签表示来解释土壤水平之间复杂的层次关系。我们的方法旨在解决复杂的分层分类,其中可能的标签数量非常大,不平衡和非平凡结构。我们在真实世界的土壤剖面数据集和领域专家的全面用户研究中证明了我们的方法的有效性。我们的经验评估表明,SoilNet可靠地预测土壤层位,是可信和准确的。用户研究结果表明,在土壤层位分类方面,SoilNet实现了与人类专家相当或更好的预测性能。所有代码和实验都可以在我们的存储库中找到:https://github.com/calgo-lab/BGR/。
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引用次数: 0
Microbial-mediated structural changes in humic acid increase phosphorus availability in the fertosphere 微生物介导的腐植酸结构变化增加了铁圈中磷的有效性
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-02-05 DOI: 10.1016/j.geoderma.2026.117713
Yanna Luo , Yunbo Niu , Wentao Pan , Lei Shi , Yingqiang Zhang , Xinxin Ye , Qizhong Xiong , Bingqiang Zhao , Jianyuan Jing
It is widely accepted that humic acid-enhanced phosphate fertilizers (HAEP) improve crop yield and phosphorus (P) use efficiency, largely because the carboxyl-rich structure of humic acid (HA) reduces P fixation and enhances soil P availability. However, this prevailing view overlooks the potential structural transformations of HA in soil and its localized regulation of P availability—restricted to the phosphate fertilizer fertosphere (PFF) rather than the bulk soil. In this study, we simulated the PFF to examine how HA influences the migration, transformation, and availability of P within the PFF. Using multispectral analysis, we characterized structural changes of HA and its corresponding ability to inhibit P immobilization within the PFF. We also identified the key microbial drivers, thereby clarifying the mechanisms through which HA regulates P availability in this microzone. Compared with that of the control, HA amendment resulted in greater migration of PFF-derived P over 90 days: cumulative migration increased by 10.7%, the migration distance extended by 0.40 mm, and the effective radius of the available P-enriched zones around the fertosphere expanded by 0.84 mm. The increased P availability might be attributed to two main mechanisms. First, the increase in HA-specific surface area and the accumulation of fungal necromass provided additional sites for ion adsorption, thereby inhibiting the transformation of P into insoluble forms. Second, HA slowed the luxury uptake of P by microorganisms, effectively reducing its biological fixation. Notably, the carboxyl functional groups of HA contributed minimally to this improvement. Instead, HA within the PFF underwent progressive depletion of carboxyl groups, mediated jointly by soil bacteria and fungi. HA enhances both the P supply area and the intensity of PFF primarily through microbial-driven structural modifications that maintain its ion-adsorption capacity. These findings offer new perspectives for re-evaluating the efficiency-enhancing mechanisms of HAEP.
腐植酸增强型磷肥(HAEP)之所以能提高作物产量和磷利用效率,很大程度上是因为腐植酸(HA)富含羧基的结构减少了磷的固定,提高了土壤磷的有效性。然而,这种流行的观点忽视了土壤中HA的潜在结构转变及其对磷有效性的局部调节-仅限于磷肥肥层(PFF)而不是整体土壤。在本研究中,我们模拟了PFF,以研究HA如何影响PFF内P的迁移、转换和可用性。通过多光谱分析,我们表征了HA的结构变化及其在PFF内抑制P固定的能力。我们还确定了关键的微生物驱动因素,从而阐明了HA调节该微区磷有效性的机制。与对照相比,HA修正导致pff衍生磷在90 d内的迁移量增加,累计迁移量增加10.7%,迁移距离延长0.40 mm,有效富磷区有效半径扩大0.84 mm。P可用性的增加可能归因于两个主要机制。首先,ha比表面积的增加和真菌坏死块的积累为离子吸附提供了额外的位点,从而抑制了P向不溶性形式的转化。其次,透明质酸减缓了微生物对磷的奢侈吸收,有效地降低了磷的生物固定。值得注意的是,HA的羧基官能团对这种改善贡献最小。相反,在土壤细菌和真菌的共同作用下,PFF内的HA经历了羧基的逐渐耗尽。HA主要通过微生物驱动的结构修饰来增强PFF的P供应面积和强度,从而保持其离子吸附能力。这些发现为重新评估HAEP的增效机制提供了新的视角。
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引用次数: 0
Enhanced weathering and biochar co-deployment boosts CO2 sequestration through changing soil properties 增强的风化和生物炭的共同部署通过改变土壤特性来促进二氧化碳的封存
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-31 DOI: 10.1016/j.geoderma.2025.117668
Emily E.E.M. te Pas, Rob N.J. Comans, Sarai Bisseling, Mathilde Hagens
Enhanced rock weathering (ERW) and biochar are potentially effective and scalable options for large-scale carbon dioxide removal (CDR), required to limit global temperature rise to 1.5 °C. Here we present experimental data on their co-deployment, an urgent and novel research direction that may render even larger CDR on multiple timescales. Two greenhouse pot experiments were conducted growing maize (Zea mays L) on sandy and clayey soils mixed with various doses of crushed dunite rocks (20–220 t ha−1) and a fixed dose of biochar (20 t ha−1) for two months. Furthermore, through a comparison of multiple soil extraction procedures for mass balance construction, our work supports the development of a standardized quantification method for CDR associated with ERW. Based on these elemental mass balances, dunite weathering was found to sequester between 1.06 ± 0.025 and 3.48 ± 0.084 t CO2 ha−1 in sandy soils and between 0.28 ± 0.015 and 1.60 ± 0.051 t CO2 ha−1 in clayey soils, while biochar co-deployment only slightly enhanced dunite weathering in the latter. Soil respiration also significantly increased on both soils, exceeding the achieved inorganic CO2 sequestration in our short-term experiments. However, we observed significant increases in soil pH and amorphous iron (hydr)oxide minerals, the latter known to be important for long-term organic carbon stabilization. We argue that the reduction in soil carbon due to enhanced soil respiration is only short term and is likely compensated for by the promising potential of ERW and biochar combinations for long-term inorganic carbon sequestration and organic carbon stabilization. The observed effects of ERW and biochar co-deployment on soil chemical properties, most notably increases in reactive (hydr)oxide minerals and soil pH, provide a great opportunity to boost CDR, with important differences between soil types.
增强岩石风化(ERW)和生物炭是大规模二氧化碳去除(CDR)的潜在有效和可扩展的选择,需要将全球气温上升限制在1.5°C。在这里,我们提出了它们共同部署的实验数据,这是一个紧迫而新颖的研究方向,可能在多个时间尺度上呈现更大的CDR。在两个温室盆栽试验中,玉米(Zea mays L)在砂质和粘土土壤上生长,混合不同剂量的碎质岩石(20 - 220 t ha - 1)和固定剂量的生物炭(20 t ha - 1),为期两个月。此外,通过对质量平衡构建的多种土壤提取方法的比较,我们的工作支持了与ERW相关的CDR标准化量化方法的发展。基于这些元素质量平衡,发现沙质土壤对沙丘风化作用的吸收在1.06±0.025 ~ 3.48±0.084 t CO2 ha - 1之间,粘质土壤对沙丘风化作用的吸收在0.28±0.015 ~ 1.60±0.051 t CO2 ha - 1之间,而黏性土壤对沙丘风化作用的吸收仅轻微增强。两种土壤的土壤呼吸也显著增加,超过了我们短期实验中实现的无机CO2固存。然而,我们观察到土壤pH值和无定形铁(水)氧化物矿物显著增加,后者已知对长期有机碳稳定很重要。我们认为,由于土壤呼吸增强而导致的土壤碳的减少只是短期的,并且可能被ERW和生物炭组合在长期无机碳固存和有机碳稳定方面的潜力所补偿。已观察到的ERW和生物炭共同部署对土壤化学性质的影响,最显著的是活性(水合)氧化物矿物质和土壤pH的增加,为提高CDR提供了很好的机会,土壤类型之间存在重要差异。
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引用次数: 0
Soil health indicators respond to management practices on commercial farms 土壤健康指标与商业农场的管理做法有关
IF 6.6 1区 农林科学 Q1 SOIL SCIENCE Pub Date : 2026-02-01 Epub Date: 2026-01-14 DOI: 10.1016/j.geoderma.2026.117674
William Osterholz , Kevin King , Margaret Kalcic , Vinayak Shedekar
Soil health is an objective of management practices including reduced tillage intensity, manure application, crop rotation, and cover crops. However, the relative effectiveness of these practices for promoting healthier soil remains uncertain. We assessed the responses of six soil health indicators (soil organic matter, soil respiration, permanganate oxidizable C (POX-C), soil protein, mean weight diameter of water stable aggregates, and bulk density) to four management practices (manure application, reduced tillage, living cover in fall and spring, and crop diversity) across 50 commercial crop fields in Ohio and Indiana, USA. Simple linear regression, multiple linear regression and random forest analyses largely identified similar relationships between soil health and management practices. Manure application rate was consistently and positively associated with greater soil health values, although the relationship with bulk density was weak. Reduced tillage intensity was associated with greater protein and respiration, but decreased POX-C. Living cover and crop diversity each had limited relationships with the soil health indicators. Soil texture was an important factor driving variability in most soil health indicators. Reducing the management period from 5 yr to 3 yr tended to reduce the predictive ability of the models, but with limited exceptions similar relationships between management and soil health were identifiable. The depth of measurement of soil health indicators changed the interpretation of management-soil health relationships in only one instance (POX-C vs. reduced tillage intensity). Overall, manure application was the most effective practice for improving soil health, with reduced tillage intensity also effective for improving several soil health indicators.
土壤健康是管理实践的一个目标,包括减少耕作强度、施用粪肥、轮作和覆盖作物。然而,这些做法在促进土壤健康方面的相对有效性仍然不确定。本研究评估了美国俄亥俄州和印第安纳州50块经济作物田6个土壤健康指标(土壤有机质、土壤呼吸、高锰酸盐可氧化C (POX-C)、土壤蛋白质、水稳定团聚体平均重径和容重密度)对4种管理措施(施肥、减少耕作、秋春季生物覆盖和作物多样性)的响应。简单线性回归、多元线性回归和随机森林分析在很大程度上确定了土壤健康与管理做法之间的类似关系。施肥量与土壤健康值呈正相关,但与容重关系较弱。减少耕作强度增加了蛋白质和呼吸,但降低了POX-C。植被覆盖和作物多样性与土壤健康指标的关系有限。土壤质地是大多数土壤健康指标变异的重要驱动因素。将管理期从5年减少到3年往往会降低模型的预测能力,但除了有限的例外,可以确定管理与土壤健康之间存在类似的关系。土壤健康指标的测量深度仅在一个实例(POX-C与减少耕作强度)中改变了对管理-土壤健康关系的解释。总体而言,施用有机肥是改善土壤健康最有效的做法,减少耕作强度对改善若干土壤健康指标也有效。
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