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XML Dataset and Benchmarks for Performance Testing of the CLS Labelling Scheme CLS标签计划的XML数据集和性能测试基准
Pub Date : 2021-07-15 DOI: 10.51984/jopas.v20i2.1243
Alhadi A. Klaib
Extensible Markup Language (XML) has become a significant technology for transferring data through the world of the Internet. XML labelling schemes are an essential technique used to handle XML data effectively. Labelling XML data is performed by assigning labels to all nodes in that XML document. CLS labelling scheme is a hybrid labelling scheme that was developed to address some limitations of indexing XML data.  Moreover, datasets are used to test XML labelling schemes. There are many XML datasets available nowadays. Some of them are from real life datasets and others are from artificial datasets. These datasets and benchmarks are used for testing the XML labelling schemes. This paper discusses and considers these datasets and benchmarks and their specifications in order to determine the most appropriate one for testing the CLS labelling scheme. This research found out that the XMark benchmark is the most appropriate choice for the testing performance of the CLS labelling scheme. 
可扩展标记语言(XML)已经成为通过Internet传输数据的重要技术。XML标记方案是用于有效处理XML数据的基本技术。标记XML数据是通过为该XML文档中的所有节点分配标签来执行的。CLS标记方案是一种混合标记方案,开发它是为了解决索引XML数据的一些限制。此外,数据集用于测试XML标记方案。现在有许多可用的XML数据集。其中一些来自真实生活数据集,另一些来自人工数据集。这些数据集和基准测试用于测试XML标记方案。本文讨论并考虑了这些数据集和基准及其规格,以确定最适合测试CLS标签方案的数据集和基准。本研究发现,XMark基准是CLS标签方案测试性能最合适的选择。
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引用次数: 1
Prevalence of Intestinal Parasites among Patients Attending Zella Central Laboratory, Zella City, Libya 利比亚Zella市Zella中心实验室患者肠道寄生虫患病率
Pub Date : 2021-07-11 DOI: 10.51984/jopas.v20i2.1352
Khdija Suleman Mohamed Ali
Intestinal parasites have become one of the most important health problems for public health experts and decision-makers in many countries. In Zella city, the prevalence of intestinal parasites was not studied before, therefore, the current study was conducted to identify the intestinal parasites in Zella city. The data in this study was collected from the records of the central laboratory in Zella city over the period of time, from the beginning of 2015, till the end of 2019. A 839 samples were examined, and 728 samples were infected with parasites, which represent 87%. The results of the study revealed the presence of four types of intestinal parasites with different proportions as follows: Entamoeba histolytica by 65%, Ascaris lumbricoides by 24%, and Entamoeba coli by 10%, and Giardia lamblia by 0.7%. The results of the study also indicate that the infection rate of males and females were very close, 86% and 87%, respectively. The results of the current study also indicate that the single type infection pattern was the most common compared to the mixed parasite infection, 95%, and 5%, respectively. The study also shows that the percentage of intestinal parasites infection was higher during the autumn and spring seasons. Despite the increase in cultural and social awareness and the improvement of the living conditions of many families, the rate of intestinal parasites infection is still high, which requires more investigations.
肠道寄生虫已成为许多国家公共卫生专家和决策者最重要的健康问题之一。Zella市肠道寄生虫的流行情况此前未见研究,因此本研究对Zella市肠道寄生虫进行鉴定。本研究中的数据收集自Zella市中心实验室在2015年初至2019年底期间的记录。共检查839份样本,其中728份样本感染寄生虫,占87%。研究结果显示,四种肠道寄生虫的存在比例不同:溶组织内阿米巴占65%,类蛔虫占24%,大肠内阿米巴占10%,贾第鞭毛虫占0.7%。研究结果还表明,男性和女性的感染率非常接近,分别为86%和87%。本研究结果还表明,与混合寄生虫感染相比,单一类型感染最常见,分别为95%和5%。研究还表明,秋季和春季肠道寄生虫感染率较高。尽管文化和社会意识的提高以及许多家庭生活条件的改善,但肠道寄生虫感染率仍然很高,这需要更多的调查。
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引用次数: 0
Green Synthesis and Characterization of Crystalline CuO Nanoparticles using Aqueous Mentha Piperita L. Leaf Extract 薄荷叶提取物绿色合成纳米CuO晶体及其表征
Pub Date : 2021-07-06 DOI: 10.51984/JOPAS.V20I2.1340
F. A. Shtewi, W. M. Al-Adiwish, Hamid A. Alqamoudy, Awatif A. Tarroush
Copper oxide nanoparticles are essential technology materials that are utilized as catalysts in the chemical industry, as well as in photonic and electronic devices and medical applications. Due to their applications in advanced technologies, we have concentrated on the production of CuO nanoparticles using enhanced, cost-effective, and environmentally friendly synthetic techniques. In this paper, we have presented a green synthesis technique to successfully synthesis copper oxide nanoparticles (CuO NPs) utilizing copper (II) sulfate pentahydrate (CuSO4.5H2O) as precursor salt and Mentha Piperita leaf extract as a reducing and stabilizing agent during the synthesis process. The precursor salt solution and reducing agent were mixed in a 1:1 volume ratio at 50 °C. The CuO NPs synthesized were confirmed by the characteristics Surface Plasmon Resonance (SPR) peak in the UV-visible region. Also, the optical direct band gap energy of the CuO NPs determined from the Tauc plot was 3.26 eV. The FTIR spectrum analysis confirmed existence of functional groups of polyphenols from Mentha piperita L. leaf extract, which are responsible for the reduction of Cu2+ ions and effective stabilization of CuO NPs. All the peaks observed in the XRD pattern revealed the production of CuO NPs having monoclinic structure with an average crystallite size of 42.51 nm. The surface morphology of the CuO nanoparticles was detected using SEM analysis. Further, the synthesis mechanism of CuO NPs has also been investigated.
氧化铜纳米颗粒是化学工业、光子和电子设备以及医疗应用中用作催化剂的重要技术材料。由于它们在先进技术中的应用,我们一直专注于使用增强的、具有成本效益的和环保的合成技术生产CuO纳米颗粒。本文以五水硫酸铜(cuo4.5 h2o)为前体盐,薄荷叶提取物为还原剂和稳定剂,采用绿色合成技术成功合成了氧化铜纳米粒子(CuO NPs)。前驱盐溶液与还原剂在50℃下以1:1的体积比混合。紫外可见区表面等离子体共振(SPR)峰的特征证实了所合成的CuO纳米粒子。此外,由tac图测定的CuO NPs的光学直接带隙能量为3.26 eV。傅里叶红外光谱分析证实,薄荷叶提取物中存在多酚官能团,这些官能团对Cu2+离子的还原和CuO NPs的有效稳定起作用。XRD谱图显示,制备的CuO纳米粒子具有单斜晶型结构,平均晶粒尺寸为42.51 nm。利用扫描电镜对纳米CuO的表面形貌进行了检测。进一步探讨了CuO NPs的合成机理。
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引用次数: 0
Analysis of a 4-span continuous plate in one direction using Matlab programming 用Matlab编程对单向四跨连续板进行分析
Pub Date : 2021-06-24 DOI: 10.4314/gjpas.v27i2.12
E. I. Adah, S. E. Ubi, F. Idagu, P. A. Ubi
The present study presents a computer approach based on polynomial shape functions application for analysis of continuous plate in one direction using Matlab. A 4-span continuous plate in the x-direction comprises of boundary conditions SSSC, SCSC, SCSC and SCSS single panels’ plate was analyzed. It was assumed that, the external edges were simply supported while the internal edges of each panel were clamped. The bending moments of the clamped edges were calculated for each panel using appropriate boundary conditions which formed the fixed end moments (FEMs). Stiffness method was used based on beam analogy to analyze the continuous plate. Matlab codes were applied to develop a computer program for this analysis. To validate the results of this present study, the values of the moments obtained were compared with those of earlier studies using manual method. The percentage difference for fixed end moments were all 0.00% and that of support moments had the maximum value of 0.016%. Thus, it was concluded that the present study program based on Matlab is adequate and a faster approach for a 4-span continuous plate in one direction analysis. Keywords: Matlab Programming, Continuous Plate, Polynomial Shape Functions, Beam Analogy, Fixed Edge Moment.
本文提出了一种基于多项式形状函数的计算机方法,在Matlab中应用于单方向连续板的分析。对由边界条件SSSC、SCSC、SCSC和SCSS单板组成的4跨x向连续板进行了分析。假设外边缘被简单支撑,而每个面板的内缘被夹紧。采用适当的边界条件计算各板夹紧边的弯矩,形成固定端矩(fem)。采用基于梁类比的刚度法对连续板进行分析。应用Matlab代码编写计算机程序进行分析。为了验证本研究的结果,用人工方法将获得的矩值与早期研究的矩值进行了比较。固定端弯矩的百分比差异均为0.00%,支座弯矩的百分比差异最大,为0.016%。由此得出结论,基于Matlab的研究程序是一种较好的四跨连续板单方向分析方法。关键词:Matlab编程,连续板,多项式形状函数,梁类比,固定边矩。
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引用次数: 0
Assessment of the interval heat flow and thermal resistance at the Faltu-1 well, Borno basin, NE Nigeria 尼日利亚东北部Borno盆地Faltu-1井层间热流和热阻评价
Pub Date : 2021-06-24 DOI: 10.4314/gjpas.v27i2.8
S. Ali, K. Mosto-Onuoha
The heat flowing through horizons in the Faltu-1 well, Borno Basin, NE Nigeria was calculated from their thermal conductivities and geothermal gradients with the aim of determining whether or not it is uniform, and if not, the depths where it is diverted, and the possible heat diversion process. The interval heat flow was assessed to be non-uniform. While fluid convection is adjudged to be the major heat diversion mechanism within the Chad Formation with minor heat refraction, the reversed is adjudged to be the situation for the underlying Kerri Kerri Formaton within which increasing sand content with depth is also predicted, with the lower interval predicted to be the Gombe Formation. Patterns of disruptions to the vertical heat flow within the Fika Formation were inferred to suggest rhythmic bedding of shale and sand beds that could serve as both source and reservoir rocks. Magmatic intrusions that impacted the maturation of organic matter into oil and gas also provided necessary entrapment structures and possible migration pathways. The Gongila and Bima Formations each has single disruption of the heat flows that are attributed to refraction. In the case of the Gongila Formation, the disruption is between the early-deposited more sandy and laterdeposited more shaley lithologies in the marine transgression of the area, while in the case of the Bima, it is between the more shaley Upper and more sandy Middle Bima Formations. Analysis of the Bullard plots also revealed disruptions to the vertical heat flow that are attributed either to convecting fluids or to heat refraction and diffraction. Two such disrupting heat advections were identified within the Chad Formation with the first being attributed to convection, while the other is attributed to a combination of both. Two similar disruptions for the Kerri Kerri Formation were attributed largely to lithological variations with minor contributions from convection of fluids. While unable to discern the rhythmic bedding, the five disruptions of the Bullard plot for the Fika Formation and one each for the Gongila and Bima Formations were interpreted to indicate similar features inferred from interval heat flow plots. Keywords: Interval heat flow, heat convection, heat diffraction, thermal resistivity, shaliness
根据尼日利亚东北部Borno盆地Faltu-1井的热导率和地温梯度计算了其流过地层的热量,目的是确定热量是否均匀,如果不均匀,则确定热量转移的深度,以及可能的热量转移过程。区间热流是非均匀的。乍得组以流体对流为主,热折射作用较小,下伏Kerri Kerri组以流体对流为主,下伏Kerri组以流体对流为主,下伏Kerri组以流体对流为主,下伏Kerri组以流体对流为主,下伏Kerri组以流体对流为主,下伏Kerri组以流体对流为主,下伏Kerri组含砂量随深度增加,下部为Gombe组。Fika组垂直热流的中断模式被推断为页岩和砂层的韵律层理,可以作为源岩和储层。岩浆侵入影响了有机质向油气的成熟,也提供了必要的圈闭结构和可能的运移路径。贡吉拉组和比马组都有单一的热流中断,这归因于折射。贡拉组在海侵早期多砂质与后期多泥质岩性之间发生断裂,比马组在上泥质岩性与中泥质岩性之间发生断裂。对Bullard图的分析还揭示了垂直热流的中断,这种中断要么归因于对流流体,要么归因于热折射和衍射。在乍得地层中发现了两个这样的破坏性热平流,第一个归因于对流,而另一个归因于两者的结合。Kerri Kerri组的两次类似破坏主要归因于岩性变化,流体对流的影响较小。虽然无法识别节奏层理,但Fika组的Bullard图的5个断裂以及贡吉拉组和比马组的各1个断裂被解释为表明了从层段热流图推断出的相似特征。关键词:区间热流,热对流,热衍射,热电阻率,泥质性
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引用次数: 0
The suitability of well water for domestic purpose, in Gwagwalada area council Abuja Nigeria 在尼日利亚阿布贾的瓜瓦拉达地区委员会,井水用于家庭用途的适用性
Pub Date : 2021-06-24 DOI: 10.4314/gjpas.v27i2.7
Ephraim Brigid Bassey, T. A. Ogah, J. I. Magaji, Oladeinde Olufemi Stephen
Water wells are usually dug by artisans who lack professional skills. Such wells are used at various homes in Gwagwalada Area Council of Abuja for domestic purpose, on the assumption that underground water is safe. Thus, the suitability of such well water was assessed. The objectives were to assess their properties and compare with regulatory standard for domestic purpose. Thirty (30) water samples were purposely collected from six wards (Dobi, Ibwa, Paikon-Kore, Tungan Maje, Zuba and Ikwa). Five water samples were randomly collected from each ward for laboratory analysis. Laboratory results were statistically analyzed using range, mean, correlation and multiple determination coefficients. Suitability of water properties for domestic purposes was determined by comparing range and mean values with NSDWQ and WHO standard for domestic uses. Result showed that the concentrations of water properties ranges as follows: temperature (26.59-30.73oC), pH (7.17-8.23), electrical conductivity EC (222-354 μs/cm3), TDS (12.14-21.54mg/l), Turbidity (0.29-0.93NTU) hardness (13.58-39.72mg/l), chloride Cl-(4.29-8.93mg/l), Fe (0.54-0.71mg/l) and Cu (0.02-0.06mg/l).Water samples generally had low electrical conductivity (222-354 μs/cm3), and mineral nutrients but elevated concentration of some heavy metals. The twenty- three water quality parameters tested could explain 96% of the overall water quality leaving only 4% unexplained. All the samples fell short of regulatory standard in terms of EC, Fe and Cu while well water from Ikwa fell short of many heavy metals for domestic purpose. It was concluded that well water in Gwagwalada Area Council are not potable. Thus, treatment before direct consumption and precautionary use of well water were recommended for the residents. Keywords: Water quality, Heavy metals, Water pollution, Well
水井通常是由缺乏专业技能的工匠挖的。在阿布贾的瓜瓦拉达地区委员会,这些井被用于家庭用途,假设地下水是安全的。因此,对该井水的适宜性进行了评价。目的是评价其性能,并与国内标准进行比较。从6个区(多比、伊布瓦、白康-科尔、东安马杰、祖巴和伊克瓦)特意采集了30个水样。每个病区随机抽取5个水样进行实验室分析。采用极差、平均值、相关性和多重决定系数对实验室结果进行统计分析。通过与NSDWQ和世卫组织家庭用水标准比较范围和平均值,确定了水的性质是否适合家庭用水。结果表明:水的性质范围为:温度(26.59 ~ 30.73 oc)、pH(7.17 ~ 8.23)、电导率EC (222 ~ 354 μs/cm3)、TDS (12.14 ~ 21.54mg/l)、浊度(0.29 ~ 0.93 ntu)、硬度(13.58 ~ 39.72mg/l)、氯化物Cl-(4.29 ~ 8.93mg/l)、铁(0.54 ~ 0.71mg/l)、铜(0.02 ~ 0.06mg/l)。水样电导率普遍较低(222 ~ 354 μs/cm3),矿质养分含量较高,部分重金属含量较高。检测的23个水质参数可以解释96%的整体水质,剩下的4%无法解释。所有样品在EC、Fe和Cu方面均未达到监管标准,而来自Ikwa的井水则未达到许多家用重金属的标准。结论是,瓜瓦拉达地区委员会的井水不适合饮用。因此,建议居民在直接饮用前进行处理,并预防使用井水。关键词:水质;重金属;水污染
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引用次数: 1
Assessing portfolio and asset returns of some financial and non- financial companies on the Ghana stock exchange using a 3-factor model 使用三因素模型评估加纳证券交易所一些金融和非金融公司的投资组合和资产回报
Pub Date : 2021-06-24 DOI: 10.4314/gjpas.v27i2.11
C. Ogbogbo, N. Anokye-Turkson
This study on the Ghana Stock Exchange (GSE), investigated, if the overall size of the market, affects the fundamentals of the Fama French 3-Factor model, and to ascertain if the Fama French model can be used effectively to assess portfolio and assets return for companies listed on the Ghana Stock Exchange. In this paper, portfolios of assets of companies on the Ghana Stock Exchange are constructed and analyzed using the Fama-French 3-factor model. The empirical data which consists of assets of 15 companies listed on the GSE, including assets of both financial and non-financial companies for good representation of the Ghana Stock Exchange. We found that the basic principle of the model is not satisfied. This is attributed to a number of factors which include overall size of the market, volume of trade, and high leverage (more debt than equity) associated with financial firms. High debt/equity ratio is linked to high risk. Keywords: Market Capitalization, Book-to-market ratio, Portfolio, Small minus big, High minus low
本研究对加纳证券交易所(GSE),调查,如果市场的整体规模,影响法玛法国3因素模型的基本面,并确定法玛法国模型是否可以有效地用于评估加纳证券交易所上市公司的投资组合和资产回报。本文采用Fama-French三因素模型对加纳证券交易所上市公司的资产组合进行了构建和分析。实证数据包括在GSE上市的15家公司的资产,包括金融和非金融公司的资产,以很好地代表加纳证券交易所。我们发现模型的基本原理是不满足的。这归因于许多因素,包括市场的总体规模、交易量和与金融公司相关的高杠杆率(债务多于股权)。高负债/权益比率与高风险有关。关键词:市值,账面市值比,投资组合,小减大,高减低
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引用次数: 0
Nurse-led training programme on knowledge about sexual violence among adolescent females in selected secondary schools, Abeokuta South local government area, Ogun state 在奥贡州阿贝奥库塔南部地方政府地区选定的中学中,由护士领导的关于青春期女性性暴力知识的培训方案
Pub Date : 2021-06-24 DOI: 10.4314/GJPAS.V27I2.15
I. Bassey, Etido Fidelis Udo, S. O. Adesite
Bryophyllum pinnatum is an important enthnomedicinal plant. The study assessed the effect of crude aqueous leaves extract of Bryophyllum pinnatum (CALEBP) on fasting plasma glucose (FPG), antioxidant status, lipid profile, liver and renal function indices in albino rats. The rats were housed under standard laboratory conditions (12h light: 12h dark photoperiod), 23± 2 oC and were given rat pellets and tap water ad libitum. Twenty four rats weighing 190-232g were randomized into four groups (A-D) of six rats each. Group A (control) received normal feed and water only. Groups B, C and D received orally 180, 360 and 540 mg/kg body weight respectively of CALEBP for 28 days. Serum aminotransferases, alkaline phosphatase (ALP), superoxide dismutase, catalase, FPG, lipid profile, urea, creatinine, bilirubin, proteins, malondialdehyde, glutathione (GSH) and total antioxidant capacity (TAC) and electrolytes were assessed by standard methods. Data were analyzed using one-way analysis of variance and p<0.05 was considered statistically significant. Groups C and D had significantly lower FPG (p = 0.030; p = 0.01) and higher ALP (p = 0.01; p = 0.001) compared to the controls. Group D had significantly lower creatinine (p = 0.03) and K+ (p = 0.02) compared to control. Group B, C and D had significantly lower GSH (p = 0.020, p = 0.000 and p = 0.000) while group B had significantly higher TAC (p = 0.04) compared to the controls. Dosage of extracts correlated positively with ALP (r = 0.705, p = 0.000) and negatively with FPG (r = -0.603, p = 0.002), K+ (r = -0.563, p = 0.004), creatinine (r = -0.464, p = 0.022) and GSH (r = -0.786, p = 0.000). Bryophyllum pinnatum aqueous leaves extract could lower blood glucose, potassium and creatinine levels and may increase ALP activity and GSH depletion in high doses. Keywords: Bryophyllum pinnatum, aqueous leaf extract, dosage, effect assessment, Albino rats
苔藓是一种重要的民族药用植物。本研究评价了青叶粗水提物(CALEBP)对白化大鼠空腹血糖(FPG)、抗氧化能力、血脂及肝肾功能指标的影响。将大鼠置于标准实验室条件下(光照12h:暗光照12h),温度23±2℃,随机给予大鼠颗粒和自来水。取体重190 ~ 232g大鼠24只,随机分为4组(A-D),每组6只。A组(对照组)仅饲喂正常饲料和水。B、C、D组分别口服CALEBP 180、360、540 mg/kg体重,疗程28 D。采用标准方法测定血清转氨酶、碱性磷酸酶(ALP)、超氧化物歧化酶、过氧化氢酶、FPG、血脂、尿素、肌酐、胆红素、蛋白质、丙二醛、谷胱甘肽(GSH)、总抗氧化能力(TAC)和电解质。资料采用单因素方差分析,p<0.05为差异有统计学意义。C、D组FPG显著降低(p = 0.030;p = 0.01)和较高的ALP (p = 0.01;P = 0.001)。与对照组相比,D组肌酐(p = 0.03)和K+ (p = 0.02)明显降低。与对照组相比,B、C、D组GSH显著降低(p = 0.020, p = 0.000, p = 0.000),而B组TAC显著升高(p = 0.04)。提取物用量与ALP (r = 0.705, p = 0.000)呈正相关,与FPG (r = -0.603, p = 0.002)、K+ (r = -0.563, p = 0.004)、肌酐(r = -0.464, p = 0.022)、GSH (r = -0.786, p = 0.000)呈负相关。苔藓叶水提物在大剂量下可降低血糖、钾和肌酐水平,增加ALP活性和GSH消耗。关键词:青叶,水提物,剂量,效果评价,白化大鼠
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引用次数: 2
Durational exposure dependent effect of carbamate treated net on haematological indices in wistar rat 氨基甲酸酯处理网对wistar大鼠血液学指标的持续暴露依赖性影响
Pub Date : 2021-06-23 DOI: 10.4314/gjpas.v27i2.4
R. Ukpanukpong, G. Ekpo, U. Aletan, P. O. Aigbadumah, P. Umoh
The present study was undertaken to investigate the duration of exposure dependent effect of carbamate treated net on haematological indices in Wistar rats. Eighteen (18) Wistar rats were used in this study and designated into three (3) study groups of six rats each. Group 1 was the control group while groups 2 and 3 constituted the experimental groups containing rats exposed to carbamate treated net for 30 and 60 days respectively. At the end of the exposure period, the animals were sacrificed and blood sample were removed for haematological analysis. The result of the study shows that there was a significant increase in the body weight of exposed rats compared to rats in the control group at (P<0.05). Relative organ weight of the exposed rats increased significantly (p<0.05) when compared to rats in the control group. The PCV, Hb, RBC’s, PLT, MCV, MCH, and MCHC levels all increased insignificantly in the exposed rats when compared to rats in the control group at (p<0.05). The study also revealed that the total White Blood Cell (WBC) counts in the exposed groups was significantly elevated when compared to the control group at (P<0.05). Finally, the result of the study shows a significant increase (p<0.05) in the levels of neutrophil of the exposed rats when compared with the control group, while the level of lymphocytes, eosinophils and monocytes increased insignificantly (p>0.05) in rats exposed for 30 days but increased significantly (p<0.05) in rats exposed for 60 days when compared to rats in their control group. Therefore, the observation from this study suggests that long-term exposure to carbamate treated net may alter the haematological indices and hence lead to various health problem. Keywords: Body Weight, Carbamate, Organs Weight and Heamatological Indices
本研究旨在探讨氨基甲酸酯处理网对Wistar大鼠血液学指标的暴露时间依赖性效应。本研究使用18只Wistar大鼠,分为三组,每组6只大鼠。第1组为对照组,第2组和第3组分别为氨基甲酸酯处理网暴露30天和60天的实验组。暴露期结束时,处死动物,取血样进行血液学分析。研究结果显示,暴露30天的大鼠体重较对照组大鼠显著增加(p<0.05),暴露60天的大鼠体重较对照组大鼠显著增加(p<0.05)。因此,本研究的观察结果表明,长期接触氨基甲酸酯处理过的蚊帐可能会改变血液学指标,从而导致各种健康问题。关键词:体重,氨基甲酸酯,器官重量,血液学指标
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引用次数: 0
Pectinolytic activity of aspergillus niger and Aspergillus flavus grown on grapefruit (citrus Parasidis) peel in solid state fermentation 葡萄柚果皮固态发酵中黑曲霉和黄曲霉的果胶溶解活性
Pub Date : 2021-06-22 DOI: 10.4314/gjpas.v27i2.2
M. Adedayo, M. Mohammed, A. Ajiboye, Sufiyan Abdulmumini
The present study was aimed at studying pectinolytic activity of resident fungi isolated from decomposing grapefruit (Citrus parasidis) peels in solid state fermentation. Grape fruit peel was subjected to natural fermentation and the fermenting fungi were isolated, characterized and identified using standard microbiological methods. The isolated fungi were in turn used for fermentation to determine their pectinolytic activity through solid state fermentation technique. Culture parameters such as incubation period, temperature, moisture content and addition of salts supplements were optimized during the research for five days. The identified fungi were Aspergillus Niger and Aspergillus flavus. The peak of pectinolytic activity was at day three of fermentation when the highest pectinase activity of 13.32 μmol/mg/min was recorded for A. Niger and 11.32 μmol/mg/min for A. flavus. Optimum temperature and pH for pectinase activity by A. Niger and A. flavus was at 40 0C and pH 7.5 and 7.7 respectively. The use of salt supplemented substrate did not alter enzyme activity. In conclusion, the isolated fungi could be promising organisms for pectinolytic enzyme production on grape peel as substrate. Keywords: Grapefruit, Pectinolytic Activity, Fungi, Fermentation, Aspergillus
本研究旨在研究从葡萄柚果皮分解中分离的常驻真菌在固态发酵中的果胶溶解活性。对葡萄果皮进行自然发酵,并采用标准微生物学方法对发酵真菌进行分离、表征和鉴定。分离的真菌依次进行发酵,通过固态发酵技术测定其果胶溶解活性。在为期5天的研究中,对培养时间、温度、水分含量和添加盐等培养参数进行了优化。鉴定的真菌为黑曲霉和黄曲霉。果胶酶活性在发酵第3天达到峰值,黑曲霉果胶酶活性最高,为13.32 μmol/mg/min,黄曲霉果胶酶活性最高,为11.32 μmol/mg/min。黑曲霉和黄曲霉果胶酶活性的最适温度和pH分别为40℃、7.5和7.7。添加盐的底物对酶活性没有影响。由此可见,分离得到的真菌是利用葡萄果皮为底物生产果胶溶酶的有前景的生物。关键词:葡萄柚,果胶溶解活性,真菌,发酵,曲霉
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引用次数: 3
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Global Journal of Pure and Applied Sciences
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