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Anti-Anaemic And Hepato–Renal Activities Of Ethanol Leaf Extract Of Alchornea Cordifolia In Phenyl Hydrazine Induced-Anaemic Wistar Rats 堇叶乙醇提取物对苯肼致贫血Wistar大鼠的抗贫血及肝肾活性
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.12
Audu Jankada P., Ezugwu Hilary C., Ipav Selumun S.
The effect of ethanol leaf extract of Alchornea cordifolia on some biochemical parameters in phenyl hydrazine-induced anaemic Wistar rats was studied. A total of thirty-six (36) Wistar rats weighing 95-200 g were selected for this study and randomly divided into six groups of six animals per group. Group A (normal control), group B (negative or anaemic) control, group C (standard), group D, E and F (treated groups). Animals in groups B, C, D, E and F were induced with anaemia via intraperitoneal (I.P.) injection of 10mg/kg body weight phenyl hydrazine (PHZ) for 3 days and group A received distilled water in place of the PHZ for the same duration. After the induction, group A and B rats received oral administration 0.9w/v normal saline solution while groups C, D, E and F received oral administration of enzoron (10mg/kg.bwt), 100, 200 and 400 mg/kg.bwt of extract respectively for 14 days. The percentage yield of the extract was determined to be 18%. Phytochemical screening revealed the presence of saponins, carbohydrates, protein, alkaloids, phytosterols, phynols, flavonoids and glycosides at varying concentrations.A dose of 5000mg/kg. bwt was found to be safe in the LD50 study of the extract. The oral administration of the extract showed a significantly  (p<0.05) level of  total protein (TP)  ,packed cell volume (PCV), haemoglobin (Hb) and red blood cells (RBCs) of the animals in treated groups  compared to those of the animals in anaemic groups.The aspartate transaminase (AST),alanine transaminase (ALT)and total bilirubin (TB)were significantly (p<0.05)  lowered in the extract treated groups than  in the anaemic non-treated groups. There was a no-significant decrease (p>0.05) in the serum urea, creatinine, Na+ ,K+  and ,Cl­- of animals in the extract treated groups when  compared to both the normal and standard control . The histology of the spleen revealed the regeneration of damaged cells in extract treated groups unlike that of the anaemic non-treated groups which showed distorted architecture. The study suggests that treatment with Achornea cordifolia leaf extract in phenyl hydrazine induced anaemia enhances anti- anaemic and hepatoprotective effect possibly due to both its anti-inflammatory and antioxidant properties.
研究了堇青花叶乙醇提取物对苯肼致贫血Wistar大鼠部分生化指标的影响。选取体重95 ~ 200 g的Wistar大鼠36只,随机分为6组,每组6只。A组(正常对照组)、B组(阴性或贫血对照组)、C组(标准组)、D、E、F组(治疗组)。B组、C组、D组、E组和F组分别腹腔注射苯基肼(phenyl hydrazine, PHZ) 10mg/kg体重诱导贫血3 D, A组用蒸馏水代替PHZ。诱导后,A、B组大鼠口服0.9w/v生理盐水,C、D、E、F组大鼠分别口服10mg/kg.bwt、100、200、400 mg/kg的恩佐隆。提取液的体重分别为14 d。提取液的提取率为18%。植物化学筛选显示,不同浓度的植物中存在皂苷、碳水化合物、蛋白质、生物碱、植物甾醇、酚类、黄酮类和苷类。剂量为5000mg/kg。在提取物的LD50研究中发现bwt是安全的。口服提取物组动物血清尿素、肌酐、Na+、K+和Cl -均显著高于正常对照组和标准对照组(p0.05)。脾脏组织学显示,提取物处理组损伤细胞再生,不同于未处理组的结构扭曲。本研究提示,用堇青叶提取物治疗苯肼所致的贫血,其抗炎和抗氧化作用可能增强了抗贫血和保护肝脏的作用。
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引用次数: 0
Preservative Effect Of Gelatin Coatings On Carrot 明胶包衣对胡萝卜的防腐作用
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.5
Ajiboye A. E., Olawoyin R. A.
Edible coating extends the life span of fresh fruits and vegetables. It is used to preserve food and prevent spoilage of fruits stored at room temperature. Preservative effect of gelatin coatings on the preservation of carrots was analyzed. Gelatin was prepared in four concentrations (0.5 g/ml, 1.0 g/ml, 1.5 g/ml and 2.0 g/ml). It was applied using dipping technique and allowed to dry before storage in sterile containers. Physiochemical parameters, weight loss and microbiological qualities of the coated and uncoated carrots were analyzed for a period of 7 days using standard procedures. Isolation and identification of bacteria was carried out using pour plate method and biochemical tests. Gelatin concentration at 0.5 g/ml attained nutrient retention with moisture content, total soluble solids and protein content of coated carrots ranging from 90.50 ± 0.01 – 85.40 ± 0.00 %, 12.20 ± 0.01 – 10.60 ± 0.00 Brixo and 1.50 ± 0.01 – 0.25 ± 0.01 % respectively. Weight loss of coated carrots at 0.5 g/ml concentration ranged from 0.35 ± 0.01 – 0.25 ± 0.02 g while 1.0 g/ml concentration ranged from 0.35 ± 0.00 – 0.28 ± 0.03 g. The bacterial and fungal counts of carrots coated with gelatin ranged between 0.20 ± 0.01 x 104 - 7.50 ± 0.05 x 104 Cfu/g and 0.20 ±0.01 x 104 – 18 ± 0.01 x 105 Cfu/g respectively. Eight bacterial and five fungal isolates namely Proteus sp., Staphylococcus sp., Enterobacter sp., Escherichia coli, Pseudomonas sp., Aeromonas sp, Bacillus sp, Streptococcus sp, Fusarium sp., Aspergillus niger, Aspergillus fumigatus, Rhizopus stolonifera, Penicillum sp. were the most occurring bacterial and fungal species respectively. Coating with gelatin extends life span, reduce water and helps maintain the phytochemical properties of the carrot.
食用涂层延长了新鲜果蔬的寿命。它被用来保存食物,防止水果在室温下变质。分析了明胶包衣对胡萝卜的保鲜效果。制备了四种浓度的明胶(0.5 g/ml、1.0 g/ml、1.5 g/ml和2.0 g/ml)。应用浸渍技术,并允许干燥,然后在无菌容器中储存。采用标准程序分析包衣和未包衣胡萝卜7 d的理化参数、失重和微生物品质。采用倾板法和生化试验对细菌进行了分离鉴定。当明胶浓度为0.5 g/ml时,包衣胡萝卜的水分含量、可溶性固形物总量和蛋白质含量分别为90.50±0.01 ~ 85.40±0.00 %、12.20±0.01 ~ 10.60±0.00 Brixo和1.50±0.01 ~ 0.25±0.01%。包膜胡萝卜在0.5 g/ml浓度下的失重范围为0.35±0.01 ~ 0.25±0.02 g, 1.0 g/ml浓度下的失重范围为0.35±0.00 ~ 0.28±0.03 g。明胶包被胡萝卜的细菌和真菌数量分别为0.20±0.01 × 104 ~ 7.50±0.05 × 104 Cfu/g和0.20±0.01 × 104 ~ 18±0.01 × 105 Cfu/g。发生最多的细菌和真菌分别为变形杆菌、葡萄球菌、肠杆菌、大肠杆菌、假单胞菌、气单胞菌、芽孢杆菌、链球菌、镰刀菌、黑曲霉、烟曲霉、stolonopus、青霉。涂上明胶可以延长胡萝卜的寿命,减少水分,并有助于保持胡萝卜的植物化学特性。
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引用次数: 0
Public Health Risk Knowledge, Awareness And Hygiene Practices Of Producers Of Artisanal Drinks Using Previously Disposed Plastic Bottles In Calabar Municipality, Cross River State, Nigeria. 尼日利亚克罗斯河州卡拉巴尔市使用先前处理过的塑料瓶的手工饮料生产者的公共卫生风险知识、意识和卫生习惯。
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.10
Obo Offiong Etim, Aniekanabasi Jonathan Okon, Olajumoke Esther Olanrewaju, Simon Alain Inah
Background: The magnitude of production and sales of unregulated beverages in Nigeria has significantly increased over the years. Several studies have found a high prevalence of pathogenic microorganisms in locally made drink samples which is a threat to public health. This study assessed the knowledge, awareness of public health risks associated with the use of previously disposed plastic bottles for packaging drinks and hygiene practices of producers of artisanal drinks in Calabar Municipality, Nigeria. Methods: The study adopted a descriptive cross-sectional design using a semi-structured questionnaire to elicit information from 166 producers of locally packaged drinks. Data collected were analyzed using SPSS version 25.0. Associations were tested using chi square statistics. Results: The study showed a high level of awareness of food borne diseases 120(73.2%). Majority of the respondents 83(50.8%) had a high level of knowledge on health risks associated with use of previously disposed bottles for packaging drinks. The level of hygiene practice was however not in tandem with Knowledge as the practice level was generally poor 99(60.3%). Majority 114(69.5%) also had a negative attitude towards safe use of previously disposed plastic bottles. There was an association between producers’ duration in business and food hygiene practices (X2= 5.153, P= 0.023). There was also a significant association between producers’ exposure to food hygiene training and food hygiene practice (X2= 49.993, P= 0.0001) Conclusion: There is an urgent need for planned regular food safety training sessions for artisanal drink producers and the regulation of sales of artisanal drinks to safeguard public health in Nigeria.
背景:多年来,尼日利亚不受管制饮料的生产和销售规模显著增加。几项研究发现,在当地制造的饮料样本中,致病微生物的流行率很高,这对公众健康构成了威胁。本研究评估了尼日利亚卡拉巴尔市手工饮料生产商对与使用以前处理过的塑料瓶包装饮料有关的公共卫生风险的知识和认识,以及他们的卫生习惯。方法:采用描述性横断面设计,采用半结构化问卷对166家当地包装饮料生产商进行问卷调查。收集的数据使用SPSS 25.0版本进行分析。使用卡方统计检验关联。结果:对食源性疾病的知晓率为120(73.2%)。大多数答复者83人(50.8%)对与使用以前处理过的瓶子包装饮料有关的健康风险有很高的了解。然而,卫生实践水平与知识水平并不一致,普遍为差99(60.3%)。114名受访者(69.5%)亦对安全使用弃置过的塑胶樽持否定态度。生产者营业时间与食品卫生行为存在相关性(X2= 5.153, P= 0.023)。生产者接受食品卫生培训与食品卫生实践之间也存在显著关联(X2= 49.993, P= 0.0001)。结论:尼日利亚迫切需要有计划地定期对手工饮料生产者进行食品安全培训,并对手工饮料的销售进行监管,以保障公众健康。
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引用次数: 0
Utilization Of Health Information Technology In Provision Of Maternal And Child Health Care Among Nurses In The University Of Calabar Teaching Hospital, Calabar 卫生信息技术在卡拉巴大学教学医院护士妇幼保健服务中的应用
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.11
A. Mathias, Aduaka Oluchukwu Stella, Enang Kingsley Oju, Agba, Affiong Mathias,
This study was assessed utilization of health information technology in provision of maternal and child health care among nurses in the University of Calabar Teaching Hospital, Calabar. The research design employed for the study was descriptive survey design. The sample of one hundred and ninety-six (196) registered nurses/midwives from the population of nurses providing maternal and child healthcare in maternity ward, children’s ward, pediatric ward, labour ward, antenatal clinic and postnatal clinic at UCTH, Calabar using simple random sampling technique. The instrument of data collection was a validated structured questionnaire. Data collected were analyzed using descriptive statistic, charts, frequencies tables and percentages. The findings of the study revealed that majority (87.2%) of nurses working in UCTH, Calabar use paper based routine health information system. The factors inhibiting utilization of health information technology among nurses to deliver maternal and child healthcare in UCTH, Calabar include: inadequate knowledge of informatics, lack of comprehensive training of nurses on ICT utilization, unavailability of health information technology tools, lack of constant power supply Based on the findings of the study the it was recommended that health policy makers, hospital managements and nurse leaders should ensure that electronic health information technology are utilized in healthcare service at UCTH, Calabar.  
本研究评估了卡拉巴大学教学医院护士在提供妇幼保健服务时使用卫生信息技术的情况。本研究采用描述性调查设计。采用简单随机抽样技术,从卡拉巴尔UCTH的产科病房、儿童病房、儿科病房、分娩病房、产前诊所和产后诊所提供妇幼保健服务的护士人口中抽取196名注册护士/助产士。数据收集的工具是一份经过验证的结构化问卷。收集的数据采用描述性统计、图表、频率表和百分比进行分析。研究结果显示,大多数(87.2%)的护士在UCTH, Calabar工作使用纸质的常规健康信息系统。卡拉巴尔市UCTH护士利用卫生信息技术提供妇幼保健服务的因素包括:信息学知识不足、护士在信息通信技术利用方面缺乏全面培训、卫生信息技术工具缺乏、缺乏持续供电。根据研究结果,建议卫生政策制定者、医院管理部门和护士领导应确保电子卫生信息技术在Calabar UCTH的卫生保健服务中得到利用。
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引用次数: 0
Assessment Of The Prevalence Of Suicide Among Young Adults Using Machine Learning 使用机器学习评估年轻人自杀率
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.7
Ibode R.T., T. A. A.,, Afeye A. F., Anifowose O. T., Owolola O. I., Ogidan O. A.
Due to the high rate of suicide all over the world resulting in about 800,000 people dying by suicide each year. The instances where suicide victims constantly publish suicide messages deliberately to express their feelings on social media, there is need to address suicide issues, and how suicide can be prevented. Therefore, as a solution to this, there is need to create a model that classifies these users" social media posts and identify users with suicidal ideations, so as to prevent future suicide cases by getting the identified users the necessary help needed. The study adopted a binary classification of a suicide-related tweet with respect to age 15 up till 29 years, on a document-level basis. A machine learning approach was employed to solve the problem of tweet classification and predictions. The dataset was generated from a Twitter API. It was observed that suicidal issues are rampant among the young adult, which need urgent attention. The paper recommended that timely intervention should be provided so as to reduce suicidal victims and preserve the future of young adults.
由于世界各地的自杀率很高,每年大约有80万人死于自杀。自杀者经常故意在社交媒体上发布自杀信息来表达自己的感受,有必要解决自杀问题,以及如何预防自杀。因此,为了解决这个问题,需要创建一个模型,对这些用户的社交媒体帖子进行分类,并识别出有自杀想法的用户,从而通过为识别出的用户提供必要的帮助来防止未来的自杀事件。该研究在文件层面上对15岁至29岁的自杀相关推文进行了二元分类。采用机器学习方法解决推文分类和预测问题。数据集是从Twitter API生成的。据观察,自杀问题在年轻人中很猖獗,需要紧急关注。论文建议,应及时提供干预,以减少自杀受害者,维护年轻人的未来。
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引用次数: 0
An Assessment Of The Impact Of Dem Interpolation Technique, Resolution, And Terrain Type On The Extraction Of Drainage Network Dem插值技术、分辨率和地形类型对流域网络提取影响的评估
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.3
A. J, Zubairu Mohammad
This research used points extracted from high-resolution DEMs (1m) to investigate the impact of resolution, interpolation method and topography on the accuracy of drainage network extraction. The investigation was conducted by evaluating the accuracy of the estimations of streams length, streams number, drainage density, and the Longitudinal Root Mean Square Error (LRMSE) of the extracted drainage networks from different DEMs interpolated using Topo to raster, Natural Neighbor (NN), kriging and IDW interpolation methods at 5, 10, 15 and 20m resolutions over moderate, steep, and gentle slope terrain. Each evaluation conducted yielded a different result, but the accuracy of the streams length estimation for most of the DEMs at all the sites increases with an increase in streams order. The total lengths of all the streams of each of the extracted networks at gentle and steep slope sites are shorter than those of the corresponding reference networks though, 15 and 20m kriging and IDW DEMs created longer streams at the moderate slope site. IDW DEMs have proven reliable for streams length estimation while Topo to raster 5, 10, and 15m for streams number estimation. In general, N.N. extracted networks are the only networks that show consistency in the streams length and number estimations, drainage density estimation as well as in LRMSE and DEM RMSE computation at all the resolutions and for all the sites. Therefore, the accuracy of N.N. DEMs and their derivatives do not rapidly change with change in resolution, especially between 5 and 20m at all (steep, gentle and moderate) terrain types.
本研究利用高分辨率dem (1m)提取的点,研究分辨率、插值方法和地形对水系网提取精度的影响。通过对不同dem在5、10、15和20米分辨率下采用Topo to raster、Natural Neighbor (NN)、kriging和IDW插值方法插值后提取的流域网络在中陡缓坡地形上的河流长度、河流数量、排水密度和纵向均方根误差(LRMSE)的估计精度进行评估。每次评估的结果都不同,但所有站点的大多数dem的流长度估计的准确性随着流顺序的增加而增加。缓坡和陡坡站点各提取网络的所有水流总长度均短于相应参考网络,而中等坡度站点的kriging和IDW dem分别为15和20m,其水流长度较长。IDW dms已被证明可以可靠地进行流长度估计,而Topo到栅格5,10和15m用于流数估计。总的来说,nnn提取的网络是唯一在所有分辨率和所有站点的河流长度和数量估计、排水密度估计以及LRMSE和DEM RMSE计算中显示一致性的网络。因此,n - n - dem及其导数的精度不会随着分辨率的变化而迅速变化,特别是在5 - 20m(陡峭、平缓和中等)地形类型之间。
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引用次数: 0
Production Of Cellulosic Enzymes By Aspergillus Niger And Hydrolysis Of Cellulosic Materials 黑曲霉生产纤维素酶及纤维素材料的水解
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.1
V. Bakare, Mohammed Sani Abdulsalami, Thankgod Ositadinma Ndibe, Chizoba Kenneth Ejuama, T. Effiong, Kereakede Ebipade
Microorganisms such as fungi can fragment carbon compounds by hydrolytic enzymes. The filamentous fungus, Aspergillus niger is now mostly considered because of its ubiquitous nature, non-fastidious nutritional requirements and it is classified generally as safe. This study was aimed at the production of cellulosic enzymes by A. niger and hydrolytic degradation of cellulosic materials by these enzymes. Standard methods were employed in soil samples collection, isolation of A. niger from the soils and their screening for enzyme production. Results showed that the A. niger isolates exhibited considerable activities of degrading and hydrolyzing cellulose in the agar media. The highest FPase, cellulase and xylanase activities were obtained from white saw dust with concentrations of 0.4059 U/ml, 0.7695U/ml and 1.3488 U/ml respectively. Also, results showed high enzyme activity at pH 6 (0.52U/ml) and temperature of 30ºC (0.72U/ml). Acid hydrolysis of the cellulosic substrates resulted to the release of 6.5% total sugar from white sawdust. The findings of this study revealed that the enzymes produced by A. niger hydrolyzed cellulosic materials but acid is more efficient than the enzymes in the hydrolysis and release of total sugar from cellulosic materials. This study recommends that cellulolytic enzymes used in the industries should be produced locally using filamentous fungus such as Aspergillus niger and cellulosic materials as carbon source.
微生物如真菌可以通过水解酶分解碳化合物。丝状真菌黑曲霉由于其普遍存在的性质,对营养的要求不高,通常被归类为安全的,所以现在主要考虑黑曲霉。本研究旨在探讨黑曲霉生产纤维素酶及其对纤维素材料的水解降解。采用标准方法采集土壤样品,从土壤中分离黑曲霉,并进行产酶筛选。结果表明,黑曲霉在琼脂培养基中具有较强的降解和水解纤维素的活性。白锯末的FPase、纤维素酶和木聚糖酶活性最高,分别为0.4059 U/ml、0.7695U/ml和1.3488 U/ml。在pH为6 (0.52U/ml)、温度为30℃(0.72U/ml)条件下,酶活性较高。纤维素底物的酸水解导致从白色锯末中释放6.5%的总糖。本研究结果表明,黑曲霉产生的酶水解纤维素材料,但酸比酶水解和释放纤维素材料中的总糖效率更高。本研究建议工业中使用的纤维素水解酶应在当地生产,使用丝状真菌如黑曲霉和纤维素材料作为碳源。
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引用次数: 0
Aninvestigation Of Winding Number Of A Closed Planar Curve 平面封闭曲线圈数的研究
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.6
W. Obeng-Denteh, Nicholas Tetteh Ofoe
This paper examines winding number of a closed planar curve through various aspects. It is related to a range on an arc in the complex plane and a point not in the range. Functions of such natures are considered to be continuous real-valued functions. Conclusion was drawn by naming a number of areas of applications.
本文从多个方面考察了平面封闭曲线的圈数。它与复平面上弧上的范围和不在范围内的点有关。具有这种性质的函数被认为是连续实值函数。通过列举一些应用领域得出结论。
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引用次数: 0
On The Calculation Of The Average Value Of The Strong Coupling Constant α_s, Using The Best Linear Unbiased Estimator (Blue) Method 用最佳线性无偏估计(Blue)方法计算强耦合常数α_s的平均值
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.13
Laamri Kallouche
Experiments to measure a single physical quantity often produce several estimates based on the same data, and which are hence correlated. We describe how to combine these correlated estimates in order to provide the best single answer, and also how to check whether the correlated estimates are mutually consistent. We discuss the properties of our technique, and illustrate its application by using it for a specific experiment which measured the strong coupling constant .In this work, we computed the mean value of the strong coupling constant by relying on the three measurement values of ATLAS. We present a method to calculate the mean value of the strong coupling constant and the uncertainty about this value at a centre of-mass energy 7Tev, 8Tev and 13Tev based on the results obtained in ref[1]. We get the result , we will compare it with the result obtained by the authors of ref[1], which is .
测量单个物理量的实验通常会根据相同的数据产生几个估计值,因此这些估计值是相关的。我们描述了如何组合这些相关的估计以提供最佳的单一答案,以及如何检查相关的估计是否相互一致。本文讨论了该技术的性质,并通过对强耦合常数测量的具体实验说明了该技术的应用。本文利用ATLAS的三个测量值计算了强耦合常数的平均值。基于文献[1]中的结果,我们提出了一种计算强耦合常数平均值和该值在质心能量为7Tev、8Tev和13Tev时的不确定性的方法。我们得到结果,我们将其与ref[1]作者得到的结果进行比较,即。
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引用次数: 0
Appraisal Of Knowledge And Practice Of Food Hygiene Among Food Vendors In University Of Calabar, Calabar, Nigeria 尼日利亚卡拉巴大学食品供应商食品卫生知识与实践评价
Pub Date : 2022-10-22 DOI: 10.4314/gjpas.v28i2.8
Easter Chukwudi Osuchukwu, Hannah Thompson Udom
Food vendors with poor knowledge and practice of food hygiene are potential source of food contamination and food borne diseases in institutions of learning in developing countries. The study was conducted to appraise the knowledge and practice of food hygiene among food vendors in the University of Calabar, Calabar, Nigeria using a cross - sectional descriptive survey as the research design. A total of sixty – seven food vendors were purposely recruited for the study.  Researcher’s developed and validated questionnaire was used for data collection. Collated data were sorted, coded, and analysed with SPSS software. The results were described using frequency counts and percentages. Findings from the study indicated that majority of the respondents 57 (85.1%) had adequate knowledge of food hygiene and the same percentage also engaged in good food hygienic practices. The result also revealed that, there was a statistically significant relationship between knowledge and practice of food hygiene (=28.134; p<0.05).  Thus, the researchers suggested continuous education of food vendors on the need to maintain hygiene practices during preparation and handling of food to safeguard the health of the students.
在发展中国家的教育机构中,缺乏食品卫生知识和实践的食品摊贩是食品污染和食源性疾病的潜在来源。本研究旨在评估尼日利亚卡拉巴大学食品供应商的食品卫生知识与实践,采用横断面描述性调查作为研究设计。总共有67名食品摊贩被有意招募参加这项研究。使用研究者开发和验证的问卷进行数据收集。整理后的资料用SPSS软件进行整理、编码和分析。结果用频率计数和百分比来描述。研究结果显示,大多数受访者(85.1%)对食物卫生有足够的认识,而同样比例的受访者亦从事良好的食物卫生守则。结果还显示,食品卫生知识与实践之间存在显著的统计学关系(=28.134;p < 0.05)。因此,研究人员建议继续教育食品供应商在准备和处理食品时需要保持卫生习惯,以保障学生的健康。
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引用次数: 0
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Global Journal of Pure and Applied Sciences
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