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The prevalence of IGF-I axis genetic polymorphisms among decathlon athletes 十项全能运动员中IGF-I轴基因多态性的患病率
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-06-01 DOI: 10.1016/j.ghir.2022.101468
Sigal Ben-Zaken , Yoav Meckel , Liina Remmel , Dan Nemet , Jaak Jürimäe , Alon Eliakim

Objective

Decathlon is a combined track and field competition, consisting of ten, mainly anaerobic events. Insulin-like growth factor-I (IGF1) axis plays a pivotal role in athletes' structural and functional muscle adaptation to exercise training, and in their competitive performance. Based on the great demand for speed physiological characteristics among decathlon athletes, the aim of this study was to assess the prevalence of IGF genetic polymorphisms among decathletes, to present an optimal genetic profile for enhancing performance.

Methods

The participants included 151 male athletes and 75 male non-athletic controls from Israel and Estonia. Athletes were divided into four groups, according to the field of expertise: (a) 40 sprinters and long jumpers; (b) 40 middle distance runners; (c) 44 Weightlifters; and (d) 27 decathletes. Genomic DNA was extracted from the participants' buccal epithelial cells using standard protocol and then analyzed for IGF1 axis related genetic polymorphism using the allelic discrimination assay.

Results

A significantly higher prevalence of the IGF1 rs35767 TT genotype was found among decathletes compared to the other athletes, as well as a lower prevalence of the IGF1 rs7136446 GG genotype, a higher prevalence of the IGF1R rs1464430 AA genotype, and a higher prevalence of the IGF2 rs680 GG genotype. Moreover, among the decathletes, carriers of the IGF1 rs7136446 GG genotype achieved higher decathlon scores compared to A-allele carriers.

Conclusions

The findings of this study suggest a potential beneficial role for some IGF-axis polymorphisms (mainly the IGF1 1245 TT and the IGF2 GG) among decathletes, both of which are associated with improved speed performance.

十项全能是一项综合田径比赛,由十个主要无氧项目组成。胰岛素样生长因子- 1 (IGF1)轴在运动员肌肉对运动训练的结构和功能适应以及竞技表现中起着关键作用。基于十项全能运动员对速度生理特征的巨大需求,本研究的目的是评估十项全能运动员中IGF基因多态性的患病率,以提出提高成绩的最佳基因谱。方法研究对象为来自以色列和爱沙尼亚的151名男性运动员和75名非运动员。运动员按专业领域分为四组:(a) 40名短跑和跳远运动员;(b) 40名中长跑运动员;(c)举重运动员44名;(d) 27名十项运动员。采用标准方案提取受试者颊上皮细胞的基因组DNA,然后采用等位基因鉴别法分析IGF1轴相关基因多态性。结果十项运动员中IGF1 rs35767 TT基因型的患病率明显高于其他运动员,IGF1 rs7136446 GG基因型的患病率较低,IGF1R rs1464430 AA基因型的患病率较高,IGF2 rs680 GG基因型的患病率较高。此外,在十项全能运动员中,IGF1 rs7136446 GG基因型携带者比a等位基因携带者获得更高的十项全能得分。本研究的结果表明,在十项运动员中,一些igf轴多态性(主要是IGF1 1245 TT和IGF2 GG)具有潜在的有益作用,这两种多态性都与速度表现的提高有关。
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引用次数: 1
Time course and reaction types of serum IGF-1 and its relationship to BMI and leptin regarding inpatients with anorexia nervosa 神经性厌食症住院患者血清IGF-1反应类型及与BMI、瘦素的关系
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-06-01 DOI: 10.1016/j.ghir.2022.101470
Christiane Hellwig-Walter , Maik Brune , Dieter Schellberg , Magdalena Buckert , Daniela Wesche , Ulrich Cuntz , Hans-Christoph Friederich , Beate Wild

Objective

Anorexia nervosa (AN) is a severe mental disorder that is characterized by restriction of energy intake, low weight, and endocrine abnormalities. One of the known endocrine changes in relation to underweight is in the GH/IGF-I axis. The aim of the study was (a) to investigate longitudinal characteristics of the IGF-I-change during therapy and weight gain in adult AN, (b) to determine relationships between IGF-I and leptin, (c) to characterize patients with weak and pronounced hormonal reactions to underweight.

Design

Data was assessed from 19 AN patients. Over the first two months, serum IGF-I concentrations were assessed on a weekly basis; thereafter on a monthly basis. The trend of IGF-I values over time was analyzed using individual growth models.

Results

In total, n = 177 IGF-I measurements were analyzed. IGF-I increased significantly dependent on BMI (slope = 20.81, p < 0.001), not modulated by duration of disease. The increase in IGF-I was significantly related to the increase in leptin concentrations over time (slope = 15.57, p < 0.001). Patients with a weaker hormonal reaction to underweight were significantly older compared to patients with a pronounced hormonal reaction (t(17) = 3.07, p = 0.007).

Conclusions

During treatment, IGF-I change is clearly related to BMI as well as to leptin. Age appears to be associated with the IGF-I response to underweight.

目的神经性厌食症(anorexia neurosa, AN)是一种以能量摄入受限、体重低、内分泌异常为特征的严重精神障碍。已知的与体重不足有关的内分泌变化之一是GH/IGF-I轴。该研究的目的是(a)调查成人AN治疗期间IGF-I变化和体重增加的纵向特征,(b)确定IGF-I和瘦素之间的关系,(c)表征对体重不足的弱而明显的激素反应的患者。DesignData从19例AN患者中进行评估。在前两个月,每周评估血清igf - 1浓度;此后按月收费。使用个体生长模型分析了IGF-I值随时间的变化趋势。结果共分析n = 177例IGF-I检测结果。IGF-I随BMI显著升高(斜率= 20.81,p <0.001),不受疾病持续时间的影响。随着时间的推移,IGF-I的增加与瘦素浓度的增加显著相关(斜率= 15.57,p <0.001)。与激素反应明显的患者相比,激素反应较弱的患者年龄明显偏大(t(17) = 3.07, p = 0.007)。结论在治疗过程中,igf - 1的变化与BMI和瘦素有明显的相关性。年龄似乎与igf - 1对体重过轻的反应有关。
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引用次数: 0
Biochemical discrepancies in the evaluation of the somatotroph axis: Elevated GH or IGF-1 levels do not always diagnose acromegaly 生长激素或IGF-1水平升高并不总是诊断肢端肥大症
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-06-01 DOI: 10.1016/j.ghir.2022.101467
Carolina Peixe , Miriam Sánchez-García , Ashley B. Grossman , Márta Korbonits , Pedro Marques

The most frequent diagnosis underlying the finding of an elevated growth hormone (GH) and insulin-like growth factor-1 (IGF-1) is acromegaly due to a GH-secreting pituitary tumour. However, GH and IGF-1 levels can be discordant in patients with acromegaly due to early or partially treated disease, or there might be another cause of high GH or high IGF-1 unrelated to acromegaly, such as pre-analytical and technical pitfalls, physiological circumstances and pathological conditions. High GH and normal or low serum IGF-1, or alternatively, normal GH with elevated serum IGF-1, should be carefully assessed to avoid misinterpreting the activity of acromegaly or misdiagnosing a patient with acromegaly. We summarise here these biochemical discrepancies in the evaluation of the somatotroph axis.

生长激素(GH)和胰岛素样生长因子-1 (IGF-1)升高最常见的诊断是由分泌GH的垂体瘤引起的肢端肥大症。然而,由于早期或部分治疗的疾病,肢端肥大症患者的GH和IGF-1水平可能不一致,或者可能存在与肢端肥大症无关的高GH或高IGF-1的其他原因,如分析前和技术缺陷、生理环境和病理条件。应仔细评估高生长激素和正常或低血清IGF-1,或者正常生长激素与血清IGF-1升高,以避免误解肢端肥大症的活动或误诊肢端肥大症患者。我们在这里总结这些生化差异在评估躯体营养轴。
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引用次数: 3
Meta-analysis of MspI derived variants of growth hormone gene associated with milk yield in dairy cattle MspI衍生的与奶牛产奶量相关的生长激素基因变异的meta分析
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-04-01 DOI: 10.1016/j.ghir.2022.101459
Yogesh C. Bangar, Ankit Magotra, A.S. Yadav, C.S. Patil

Objective

The present work aimed to obtain common effect sizes for the gene frequency and association of MspI derived variants of growth hormone (GH) gene with milk yield in dairy cows.

Methods

We performed a systematic review and meta-analysis of 35 published studies identified in literature search from 2000 to 2020 (n = 4164). These studies were specific to fragment size (329) for genotypes viz., CC (224, 105 bp), CD (329, 224, 105 bp) and DD (329 bp). Pooled standard mean differences (SMDs) as effect sizes between allele pairs were derived using different genetic models. The heterogeneity between effects sizes across studies was estimated using I2 Index (%).

Results

The common effect size for gene frequency of allele C (224, 105 bp) was significantly (P < 0.05) higher in 2881 Bos taurus/cross cows (0.82; 95% CI: 0.74, 0.89; I2 = 97.81%) than 1283 Bos indicus cows (0.15; 95% CI: 0.12, 0.18; I2 = 71.90%), with overall gene frequency was 0.33 (95% CI: 0.21, 0.46; I2 = 99.29%). Additive (CC vs. DD) and dominant (CC + CD vs. DD) did not revealed significant (P > 0.05) association with milk yield. However, completely over dominant (CC + DD vs. CD) and recessive (CC vs. CD + DD) models showed significant (P < 0.05) and positive SMDs with milk yield specially at early lactations. There was no evidence of heterogeneity (I2 = 0.00%) between SMDs across studies.

Conclusions

This meta-analysis suggested potential association of C allele for enhancing milk production of dairy cows.

目的研究奶牛生长激素(GH)基因MspI衍生变异的基因频率及其与产奶量的关系。方法:我们对2000年至2020年文献检索中已发表的35项研究(n = 4164)进行了系统回顾和荟萃分析。这些研究是针对基因型的片段大小(329)进行的,即CC (224, 105 bp), CD (329, 224, 105 bp)和DD (329 bp)。利用不同的遗传模型推导出等位基因对间的效应大小。使用I2指数(%)估计各研究间效应大小的异质性。结果等位基因C基因频率的共同效应量(224,105 bp)显著(P <2881头牛/杂交牛(0.82;95% ci: 0.74, 0.89;I2 = 97.81%)高于1283头母牛(0.15;95% ci: 0.12, 0.18;I2 = 71.90%),总基因频率为0.33 (95% CI: 0.21, 0.46;i2 = 99.29%)。加性(CC vs. DD)和显性(CC + CD vs. DD)无显著差异(P >0.05)与产奶量相关。然而,完全过显性(CC + DD vs. CD)和隐性(CC vs. CD + DD)模型显示显著(P <0.05), smd阳性与泌乳量有关,尤其是在泌乳早期。没有证据表明不同研究的smd之间存在异质性(I2 = 0.00%)。结论本荟萃分析提示C等位基因与奶牛产奶量的潜在关联。
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引用次数: 0
The role of growth hormone for fertility in women with hypopituitarism 生长激素在垂体功能低下妇女生育中的作用
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-04-01 DOI: 10.1016/j.ghir.2022.101458
Julie Chen , Laurence Katznelson

Growth hormone (GH) is an important regulator of the female reproductive system. In vitro and non-human in vivo studies demonstrate a role of GH in steroidogenesis, folliculogenesis, and post-fertilization development. Given its ability to modulate the reproductive system and potentiate the effects of gonadotropins, a beneficial role of GH replacement therapy to optimize fertility has been suggested. Women with hypopituitarism have lower pregnancy and live birth rates. Limited data suggest a role of GH in enhancing fertility management in women with hypopituitarism. GH replacement therapy may be especially relevant in women with hypopituitarism as well as in women considered poor ovarian responders and require assisted reproductive techniques.

生长激素(GH)是女性生殖系统的重要调节因子。体外和非人类体内研究表明生长激素在类固醇生成、卵泡生成和受精后发育中的作用。鉴于其调节生殖系统和增强促性腺激素作用的能力,已经提出了生长激素替代疗法在优化生育能力方面的有益作用。垂体功能减退的妇女怀孕率和活产率较低。有限的数据表明生长激素在增强垂体功能低下妇女生育管理中的作用。生长激素替代疗法可能特别适用于垂体功能低下的妇女,以及被认为卵巢反应不良和需要辅助生殖技术的妇女。
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引用次数: 0
Liver and muscle-specific effects of phoenixin-20 on the insulin-like growth factor system mRNAs in zebrafish 凤凰素-20对斑马鱼胰岛素样生长因子系统mrna的肝脏和肌肉特异性影响
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-04-01 DOI: 10.1016/j.ghir.2022.101456
Jithine Jayakumar Rajeswari , Emilio J. Vélez , Suraj Unniappan

Objective

Phoenixin-20 (Pnx-20) is a bioactive peptide with endocrine-like actions in vertebrates. Recent studies suggest Pnx-20 promotes growth hormone/insulin-like growth factors (Gh/Igf) axis, an important endocrine regulator of growth in mammals and fish.

Design

In this research, we determined whether Pnx-20 affects the different members of the Igf family, its binding proteins and receptors (Igf-system) in zebrafish liver and muscle.

Results

In vivo administration of Pnx-20 downregulated igfs, igf receptors (igfrs) and igf binding protein (igfbp) 5 mRNA expression in the liver of male and female zebrafish at both 1 and 6 h post-intraperitoneal (IP) injection. Interestingly, this effect occurred at a relatively earlier timepoint in female zebrafish suggesting sex-specific differences in Pnx-20 action. Besides, either 6 or 24 h in vitro incubations with Pnx-20 downregulated the expression of all igfs, igfrs and igfbp5 mRNAs (except igf2a) analyzed in a zebrafish liver cell (ZFL) line. Moreover, siRNA-mediated knockdown of Pnx-20 upregulated all Igf-system mRNAs analyzed in ZFL cells. Together, these results (both in vivo and in vitro) revealed a general suppressive action for both endogenous and exogenous Pnx-20 on the hepatic Igf-system of zebrafish. In contrast, a general sex-specific upregulation of the Igf-system mRNAs analyzed was found in the muscle of Pnx-20 injected fish. Future research should explore the sex- and time-differences observed in the present study.

Conclusions

Collectively, this research shows that Pnx-20 is a tissue-specific regulator of the liver (suppressor) and muscle (stimulant) Igf signaling in both male and female zebrafish.

目的研究凤凰素-20 (phoenixin -20, Pnx-20)是一种具有内分泌样作用的生物活性肽。最近的研究表明,Pnx-20促进生长激素/胰岛素样生长因子(Gh/Igf)轴,这是哺乳动物和鱼类生长的重要内分泌调节因子。在本研究中,我们确定了Pnx-20是否影响斑马鱼肝脏和肌肉中Igf家族的不同成员、其结合蛋白和受体(Igf系统)。结果Pnx-20在腹腔注射后1和6 h下调雄性和雌性斑马鱼肝脏中igfs、igf受体(igfrs)和igf结合蛋白(igfbp) 5mrna的表达。有趣的是,这种效应发生在雌性斑马鱼相对较早的时间点,这表明Pnx-20的作用存在性别特异性差异。此外,Pnx-20体外培养6或24小时后,斑马鱼肝细胞(ZFL)中除igf2a外,所有igfs、igfrs和igfbp5 mrna的表达均下调。此外,sirna介导的Pnx-20的下调上调了ZFL细胞中分析的所有igf系统mrna。总之,这些结果(体内和体外)揭示了内源性和外源性Pnx-20对斑马鱼肝脏igf系统的普遍抑制作用。相比之下,在注射Pnx-20的鱼的肌肉中发现了igf系统mrna的性别特异性上调。未来的研究应该探索在本研究中观察到的性别和时间差异。总之,本研究表明Pnx-20在雄性和雌性斑马鱼中都是肝脏(抑制因子)和肌肉(刺激因子)Igf信号的组织特异性调节因子。
{"title":"Liver and muscle-specific effects of phoenixin-20 on the insulin-like growth factor system mRNAs in zebrafish","authors":"Jithine Jayakumar Rajeswari ,&nbsp;Emilio J. Vélez ,&nbsp;Suraj Unniappan","doi":"10.1016/j.ghir.2022.101456","DOIUrl":"10.1016/j.ghir.2022.101456","url":null,"abstract":"<div><h3>Objective</h3><p>Phoenixin-20 (Pnx-20) is a bioactive peptide with endocrine-like actions in vertebrates. Recent studies suggest Pnx-20 promotes growth hormone/insulin-like growth factors (Gh/Igf) axis, an important endocrine regulator of growth in mammals and fish.</p></div><div><h3>Design</h3><p>In this research, we determined whether Pnx-20 affects the different members of the Igf family, its binding proteins and receptors (Igf-system) in zebrafish liver and muscle.</p></div><div><h3>Results</h3><p><em>In vivo</em> administration of Pnx-20 downregulated <em>igfs</em>, <em>igf</em> receptor<em>s</em> (<em>igfrs</em>) and <em>igf</em> binding protein (<em>igfbp</em>) 5 mRNA expression in the liver of male and female zebrafish at both 1 and 6 h post-intraperitoneal (IP) injection. Interestingly, this effect occurred at a relatively earlier timepoint in female zebrafish suggesting sex-specific differences in Pnx-20 action. Besides, either 6 or 24 h <em>in vitro</em> incubations with Pnx-20 downregulated the expression of all <em>igfs</em>, <em>igf</em>rs and <span><em>igfbp5</em></span> mRNAs (except <em>igf2a</em>) analyzed in a zebrafish liver cell (ZFL) line. Moreover, siRNA-mediated knockdown of Pnx-20 upregulated all Igf-system mRNAs analyzed in ZFL cells. Together, these results (both <em>in vivo</em> and <em>in vitro</em>) revealed a general suppressive action for both endogenous and exogenous Pnx-20 on the hepatic Igf-system of zebrafish. In contrast, a general sex-specific upregulation of the Igf-system mRNAs analyzed was found in the muscle of Pnx-20 injected fish. Future research should explore the sex- and time-differences observed in the present study.</p></div><div><h3>Conclusions</h3><p>Collectively, this research shows that Pnx-20 is a tissue-specific regulator of the liver (suppressor) and muscle (stimulant) Igf signaling in both male and female zebrafish.</p></div>","PeriodicalId":12803,"journal":{"name":"Growth Hormone & Igf Research","volume":"63 ","pages":"Article 101456"},"PeriodicalIF":1.4,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77836395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The true story of the “strong and gentle” Acciano's Giant “坚强而温柔”的阿齐亚诺的巨人的真实故事
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-04-01 DOI: 10.1016/j.ghir.2022.101457
Maria Maddalena Sirufo , Lina Maria Magnanimi , Lia Ginaldi , Massimo De Martinis

This is the story of a giant who lived in Abruzzo 200 years ago. He became a symbol for his people and a strong resilience generator. Gigantism, in the history of humanity has always attracted attention, albeit passing over the centuries from myth, from divinity to the freak phenomenon, the freak of nature that becomes a spectacle to show off. The attraction for understanding the pathophysiological mechanisms underlying gigantism developed by the end of 19th century. Increased levels of growth hormone (GH) or insulin-like growth hormone 1 (IGF1) causes overgrowth in pituitary gigantism. The imposing size of the body, in our imagination, represents strength and health, reason why in our imagination it almost becomes a divine mythical image. The story of the Acciano's Giant represents a cultural heritage that passes from one generation to the next, that contributes in giving a sense of identity and continuity. It provides a link from past to present and to the future. Encourages a sense of identity and responsibility contributing to social cohesion, helping individuals to feel members of one community. A disease, represented by the Giant, has become a symbol capable of bringing the community together and giving it the strength to react to environment, nature and history. This is a lesson that teaches us the sense of community.

这是200年前住在阿布鲁佐的一个巨人的故事。他成为了他的人民的象征,一个强大的恢复力发电机。巨人症,在人类历史上一直吸引着人们的注意,尽管经历了几个世纪,从神话,从神性到怪异的现象,自然的怪异,成为炫耀的奇观。对巨人症的病理生理机制的理解是在19世纪末发展起来的。生长激素(GH)或胰岛素样生长激素1 (IGF1)水平升高导致垂体巨人症过度生长。在我们的想象中,雄伟的身躯代表着力量和健康,这就是为什么在我们的想象中,它几乎成为一个神圣的神话形象。阿齐亚诺的《巨人》的故事代表了一种文化遗产,从一代传到下一代,这有助于给人一种认同感和连续性。它提供了从过去到现在和到未来的联系。鼓励认同感和责任感,有助于社会凝聚力,帮助个人感觉自己是一个社区的成员。一种疾病,以巨人为代表,已经成为一种象征,能够将社区团结在一起,并赋予它应对环境、自然和历史的力量。这是教会我们社区意识的一课。
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引用次数: 0
Hormonal, metabolic, and angiogenic responses to all-out sprint interval exercise under systemic hyperoxia 全身性高氧条件下全速冲刺间歇运动的激素、代谢和血管生成反应
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-04-01 DOI: 10.1016/j.ghir.2022.101445
Michihiro Kon , Yoshiko Ebi , Kohei Nakagaki

Objective

Hyperoxic gas inhalation during exercise may negatively affect all-out sprint interval exercise (SIE)-induced hormonal, metabolic, and angiogenic responses. We investigated the effects of acute all-out SIE under systemic hyperoxia on hormonal, metabolic, and angiogenic responses.

Design

This was a randomised-crossover trial. Ten healthy males (mean ± standard error of age = 23.1 ± 0.9 years; height = 171.0 ± 1.6 cm; body mass = 66.2 ± 2.0 kg; body mass index = 22.6 ± 0.5 kg/m2) completed the following two experimental regimens: 1) SIE under normoxia and 2) SIE under systemic hyperoxia (FiO2 = 60%). The subjects performed four bouts of 30-s maximal cycling efforts with 4 min recovery between efforts. The circulating levels of hormonal (growth hormone, epinephrine, and norepinephrine), metabolic (glucose, free fatty acid, and lactate), and angiogenic (vascular endothelial growth factor, matrix metalloproteinase-2 and -9, and endostatin) markers were measured before and at 0 (immediately after the regimen), 30, and 120 min after both regimens.

Results

In response to both SIE regimens, the peak and mean power outputs gradually decreased over the intermittent exercise session compared with those in the first bout (p < 0.01) with no significant differences between the regimens. Both regimens significantly increased the circulating concentrations of all hormonal, metabolic, and angiogenic markers (p < 0.01). However, there were no significant differences in the levels of these markers in response to the two regimens at any time point (p > 0.05).

Conclusion

These findings suggest that acute systemic hyperoxia does not influence the hormonal, metabolic, and angiogenic responses to all-out SIE.

目的运动时高氧气体吸入可能对全速冲刺间歇运动(SIE)诱导的激素、代谢和血管生成反应产生负面影响。我们研究了全身高氧条件下急性全面SIE对激素、代谢和血管生成反应的影响。这是一项随机交叉试验。健康男性10例(年龄平均±标准误差= 23.1±0.9岁;高度= 171.0±1.6 cm;体重= 66.2±2.0 kg;体重指数= 22.6±0.5 kg/m2)完成了两个实验方案:1)常氧下SIE和2)全身高氧(FiO2 = 60%)下SIE。受试者进行了四组30秒的最大自行车运动,每次运动之间的恢复时间为4分钟。在两种治疗方案开始前和治疗后0分钟(治疗后立即)、30分钟和120分钟测量激素(生长激素、肾上腺素和去甲肾上腺素)、代谢(葡萄糖、游离脂肪酸和乳酸)和血管生成(血管内皮生长因子、基质金属蛋白酶-2和-9、内皮抑素)标志物的循环水平。结果在两种SIE方案的反应中,间歇运动期间的峰值和平均功率输出与第一回合相比逐渐下降(p <0.01),两组间无显著差异。两种方案都显著增加了所有激素、代谢和血管生成标志物的循环浓度(p <0.01)。然而,在两种方案的任何时间点,这些标志物的水平没有显著差异(p >0.05)。结论急性全身性高氧不影响全负荷SIE的激素、代谢和血管生成反应。
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引用次数: 0
Effects of high-dose all-trans retinoic acid on longitudinal bone growth of young rats 大剂量全反式维甲酸对幼鼠纵向骨生长的影响
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-02-01 DOI: 10.1016/j.ghir.2022.101446
Qin Shen , Xia Wang , Haodi Bai , Xin Tan , Xing Liu

Objective

The signaling axis consisting of GH-IGF1-IGFBP3 is the primary signal taht acts prepubertally to influence height development. Growth plate thinning and even premature closure have been reported in children with tumors treated with retinoid chemotherapy, resulting in long bone dysplasia. Growth failure may occur despite received GH treatment, but the reason is unknown. This study investigate the effect of high-dose all-trans retinoic acid (ATRA) on the development of long bones in growing SD rats.

Methods

A total of 20 three-week-old male SD rats were randomly divided into a control group and an experimental group (n = 10). Rats were treated by gavage with or without high-dose ATRA for 10 days. The body weights of the rats were recorded daily. At the end of the experiment, we measured the length of nose-tail and tibia, stained the tibia and liver for pathological tissue and RT-PCR reaction, and measured the levels of serum GH, IGF1 and IGFBP3, and so on.

Results

Compared with controls, experimental rats exhibited reduced body weight and shortened nasal-tail and radial tibial length. Cyp26b1 enzyme activity in the liver was elevated, and histopathological staining revealed that the cartilaginous epiphyseal plate was narrowed, the medullary cavity of trabecular bone was sparse, the number of trabecular bones was decreased, trabecular separation was increased, bone marrow mineralization was enhanced, osteoclastic activity was increased, and circulating GH-IGF1-IGFBP3 levels were decreased. However, RT-PCR reaction results of localized proximal tibiae showed upregulation of IGF1 and downregulation of IGFBP3.

Conclusions

High-dose ATRA intake over a short period of time can reduce GH-IGF1-IGFBP3 levels, affect cartilage and bone homeostasis, and inhibit bone growth in developing animals.

目的GH-IGF1-IGFBP3信号轴是青春期前影响身高发育的主要信号。有报道称,在接受类维甲酸化疗的儿童肿瘤中,生长板变薄甚至过早闭合,导致长骨发育不良。尽管接受生长激素治疗,仍可能发生生长衰竭,但原因尚不清楚。本研究探讨了大剂量全反式维甲酸(ATRA)对生长SD大鼠长骨发育的影响。方法选用3周龄雄性SD大鼠20只,随机分为对照组和试验组(n = 10)。大鼠分别给予或不给予高剂量ATRA灌胃10 d。每天记录大鼠体重。实验结束后测量鼻尾和胫骨长度,对胫骨和肝脏进行病理组织染色和RT-PCR反应,测定血清GH、IGF1、IGFBP3水平等。结果与对照组相比,实验组大鼠体重减轻,鼻尾和桡胫长度缩短。肝脏Cyp26b1酶活性升高,组织病理学染色显示软骨骺板变窄,骨小梁髓腔稀疏,骨小梁数量减少,骨小梁分离增加,骨髓矿化增强,破骨活性增加,循环GH-IGF1-IGFBP3水平降低。然而,局部胫骨近端RT-PCR反应结果显示IGF1上调,IGFBP3下调。结论短时间内摄入高剂量ATRA可降低发育动物GH-IGF1-IGFBP3水平,影响软骨和骨骼稳态,抑制骨骼生长。
{"title":"Effects of high-dose all-trans retinoic acid on longitudinal bone growth of young rats","authors":"Qin Shen ,&nbsp;Xia Wang ,&nbsp;Haodi Bai ,&nbsp;Xin Tan ,&nbsp;Xing Liu","doi":"10.1016/j.ghir.2022.101446","DOIUrl":"10.1016/j.ghir.2022.101446","url":null,"abstract":"<div><h3>Objective</h3><p>The signaling axis consisting of GH-IGF1-IGFBP3 is the primary signal taht acts prepubertally to influence height development. Growth plate<span><span><span><span> thinning and even premature closure have been reported in children with tumors treated with retinoid chemotherapy, resulting in long </span>bone dysplasia. Growth failure may occur despite received GH </span>treatment<span>, but the reason is unknown. This study investigate the effect of high-dose all-trans retinoic acid (ATRA) on the development of long bones in growing </span></span>SD rats.</span></p></div><div><h3>Methods</h3><p>A total of 20 three-week-old male SD rats were randomly divided into a control group and an experimental group (<em>n</em><span> = 10). Rats were treated by gavage with or without high-dose ATRA for 10 days. The body weights of the rats were recorded daily. At the end of the experiment, we measured the length of nose-tail and tibia<span>, stained the tibia and liver for pathological tissue<span><span> and RT-PCR reaction, and measured the levels of serum GH, IGF1 and </span>IGFBP3, and so on.</span></span></span></p></div><div><h3>Results</h3><p>Compared with controls, experimental rats exhibited reduced body weight and shortened nasal-tail and radial tibial length. Cyp26b1 enzyme activity<span><span><span> in the liver was elevated, and histopathological staining revealed that the cartilaginous epiphyseal plate was narrowed, the medullary cavity of trabecular bone was sparse, the number of trabecular bones was decreased, trabecular separation was increased, bone marrow </span>mineralization was enhanced, osteoclastic activity was increased, and circulating GH-IGF1-IGFBP3 levels were decreased. However, RT-PCR reaction results of localized </span>proximal tibiae showed upregulation of IGF1 and downregulation of IGFBP3.</span></p></div><div><h3>Conclusions</h3><p>High-dose ATRA intake over a short period of time can reduce GH-IGF1-IGFBP3 levels, affect cartilage and bone homeostasis, and inhibit bone growth in developing animals.</p></div>","PeriodicalId":12803,"journal":{"name":"Growth Hormone & Igf Research","volume":"62 ","pages":"Article 101446"},"PeriodicalIF":1.4,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39911457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Dental arches in inherited severe isolated growth hormone deficiency 遗传性严重分离生长激素缺乏症的牙弓
IF 1.4 4区 医学 Q4 CELL BIOLOGY Pub Date : 2022-02-01 DOI: 10.1016/j.ghir.2022.101444
Rafaela S. Girão , Manuel H. Aguiar-Oliveira , Bruna M.R. Andrade , Marcos A.V. Bittencourt , Roberto Salvatori , Evânio V. Silva , André L.M. Santos , Matheus M. Cunha , Wilton M. Takeshita , Alaíde H.A. Oliveira , Eugênia H.O. Valença , Alécia A. Oliveira-Santos , Luiz A. Oliveira-Neto

Objectives

The growth of the dental arches depends on GH and insulin-like growth factor type 1 (IGF1), but the consequences of GH deficiency (GHD) on their growth are still unclear, probably due to the acquired etiology of GHD in most described series, often associated with additional pituitary deficits (thyrotrophic, corticotrophic and gonadotrophic hormones), and imperfections of related replacement therapies, which may affect the dental arch growth. To avoid these limitations, we took advantage of a unique cohort of subjects with isolated GH deficiency (IGHD) due the same mutation in the GH releasing hormone receptor gene, living with very low serum GH and low to undetectable circulating IGF1 levels. Our purpose was to analyze the dimensions of maxillary and mandibular dental arches.

Methods

22 adult IGHD (15 untreated and 7 previously partially treated with GH) and 33 controls were enrolled in a cross-sectional study using the Ortho Insight 3D and MeshMixer software,

Results

In untreated IGHD subjects all maxillary arch measures were smaller than controls, while among mandibular arches, only the mandibular canine width and the mandibular arch length were reduced. In partially GH treated subjects only the palate depth, the maxillary canine width, the maxillary and mandibular arch lengths remained smaller than controls.

Conclusions

IGHD reduces the growth of maxillary arch to a greater degree than the mandibular arch, suggesting different control of superior and inferior dental arches. GH treatment increases some of these measures.

目的牙弓的生长依赖于生长激素和胰岛素样生长因子1型(IGF1),但生长激素缺乏(GHD)对牙弓生长的影响尚不清楚,可能是由于大多数描述的GHD的获得性病因,通常与额外的垂体缺陷(甲状腺、促皮质和促性腺激素)以及相关替代疗法的不完善有关,这可能会影响牙弓的生长。为了避免这些局限性,我们利用了一组独特的研究对象,这些研究对象是由于生长激素释放激素受体基因的相同突变而孤立的生长激素缺乏症(IGHD),他们的血清生长激素非常低,循环IGF1水平低到无法检测。我们的目的是分析上颌和下颌牙弓的尺寸。方法采用Ortho Insight 3D和MeshMixer软件对22名成人IGHD患者(15名未治疗,7名曾部分接受GH治疗)和33名对照组进行横断面研究。结果未治疗的IGHD患者上颌弓的测量值均小于对照组,而在下颌弓中,只有下颌犬齿宽度和下颌弓长度减小。在部分GH治疗的受试者中,只有上颚深度、上颌犬齿宽度、上颌和下颌弓长度比对照组小。结论sigd对上颌弓生长的抑制作用大于对下颌骨弓生长的抑制作用,说明对上下牙弓的控制是不同的。生长激素治疗增加了这些措施中的一些。
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引用次数: 4
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Growth Hormone & Igf Research
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