Pub Date : 2024-08-16DOI: 10.1016/j.hpj.2024.03.008
Zhiran Wu, Qianqian Dang, Shuni Ouyang, Wei Liu, Lili Huang
Ambient temperature affects the occurrence and prevalence of plant disease. Most bacterial diseases are damaging at high temperatures. However, kiwifruit bacterial canker caused by pv. () has been found to be prevalent at relatively cool temperatures, and it is unclear how ambient temperature affects the development of kiwifruit bacterial canker. In this study, basal resistance to was suppressed in kiwifruit at cool growth temperature (16 °C) compared with at normal temperature (24 °C). In addition, RNA sequence analysis and ethylene content assessment indicated that ethylene modulated kiwifruit resistance to at normal growth temperature and that cool temperature inhibited ethylene accumulation and -induced activation of the ethylene signaling pathway in kiwifruit. Virus-mediated silencing of the kiwifruit ethylene signaling gene suppressed kiwifruit resistance to at normal growth temperature. Exogenous application of ethylene inhibitor 1-methylcyclopropene eliminated the difference in kiwifruit resistance to at 16 and 24 °C. Exogenous application of ethylene analogues ethephon induced resistance to in kiwifruit. In conclusion, cool temperatures impair basal resistance to by reducing the activation of ethylene biosynthesis and signaling in kiwifruit. The results provide clues for new strategies to control plant diseases in a context of global environmental change.
{"title":"Ethylene-mediated resistance to bacterial canker in kiwifruit is suppressed by cool temperature","authors":"Zhiran Wu, Qianqian Dang, Shuni Ouyang, Wei Liu, Lili Huang","doi":"10.1016/j.hpj.2024.03.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.03.008","url":null,"abstract":"Ambient temperature affects the occurrence and prevalence of plant disease. Most bacterial diseases are damaging at high temperatures. However, kiwifruit bacterial canker caused by pv. () has been found to be prevalent at relatively cool temperatures, and it is unclear how ambient temperature affects the development of kiwifruit bacterial canker. In this study, basal resistance to was suppressed in kiwifruit at cool growth temperature (16 °C) compared with at normal temperature (24 °C). In addition, RNA sequence analysis and ethylene content assessment indicated that ethylene modulated kiwifruit resistance to at normal growth temperature and that cool temperature inhibited ethylene accumulation and -induced activation of the ethylene signaling pathway in kiwifruit. Virus-mediated silencing of the kiwifruit ethylene signaling gene suppressed kiwifruit resistance to at normal growth temperature. Exogenous application of ethylene inhibitor 1-methylcyclopropene eliminated the difference in kiwifruit resistance to at 16 and 24 °C. Exogenous application of ethylene analogues ethephon induced resistance to in kiwifruit. In conclusion, cool temperatures impair basal resistance to by reducing the activation of ethylene biosynthesis and signaling in kiwifruit. The results provide clues for new strategies to control plant diseases in a context of global environmental change.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142090520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The pear ( spp.) is well known for diverse flavors, textures, and global horticultural importance. However, the genetic diversity responsible for its extensive phenotypic variations remains largely unexplored. Here, we assembled and annotated the genomes of the maternal (PsbM) and paternal (PsbF) lines of the hybrid ‘Yuluxiang’ pear and constructed the pear pangenome of 1.15 Gb by combining these two genomes with five previously published pear genomes representing cultivated and wild germplasm. Using the constructed pangenome, we identified 21 224 gene PAVs (Presence-absence variation) and 1 158 812 SNPs (Single Nucleotide Polymorphism) in the non-reference genome that were absent in the PsbM reference genome. Compared with SNP markers, PAV-based analysis provides additional insights into the pear population structure. In addition, some genes associated with pear fruit quality traits have differential occurrence frequencies and differential gene expression between Asian and European populations. Moreover, our analysis of the pear pangenome revealed a mutated SNP and an insertion in the promoter region of the gene potentially enhance sepal shedding in ‘Xuehuali’ which is vital for pear quality. may play a role in the IAA pathway, contributing to a distinct low-auxin phenotype observed in plants by heterologously overexpressing this gene. This research helps capture the genetic diversity of pear populations and provides genomic resources for accelerating breeding.
{"title":"Pear genomes display significant genetic diversity and provide novel insights into the fruit quality traits differentiation","authors":"Baopeng Ding, Haifei Hu, Yunpeng Cao, Ruirui Xu, Yujing Lin, Tahir ul Qamar Muhammad, Yuqin Song, Guangqi He, Youzhi Han, Huangping Guo, Jun Qiao, Jianguo Zhao, Xinxin Feng, Sheng Yang, Xuhu Guo, Rajeev Kumar Varshney, Liulin Li","doi":"10.1016/j.hpj.2024.05.005","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.05.005","url":null,"abstract":"The pear ( spp.) is well known for diverse flavors, textures, and global horticultural importance. However, the genetic diversity responsible for its extensive phenotypic variations remains largely unexplored. Here, we assembled and annotated the genomes of the maternal (PsbM) and paternal (PsbF) lines of the hybrid ‘Yuluxiang’ pear and constructed the pear pangenome of 1.15 Gb by combining these two genomes with five previously published pear genomes representing cultivated and wild germplasm. Using the constructed pangenome, we identified 21 224 gene PAVs (Presence-absence variation) and 1 158 812 SNPs (Single Nucleotide Polymorphism) in the non-reference genome that were absent in the PsbM reference genome. Compared with SNP markers, PAV-based analysis provides additional insights into the pear population structure. In addition, some genes associated with pear fruit quality traits have differential occurrence frequencies and differential gene expression between Asian and European populations. Moreover, our analysis of the pear pangenome revealed a mutated SNP and an insertion in the promoter region of the gene potentially enhance sepal shedding in ‘Xuehuali’ which is vital for pear quality. may play a role in the IAA pathway, contributing to a distinct low-auxin phenotype observed in plants by heterologously overexpressing this gene. This research helps capture the genetic diversity of pear populations and provides genomic resources for accelerating breeding.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142025157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The HD-Zip transcription factor LcATHB15 is involved in litchi seed development by promoting the expression of the splicing regulator LcSR45","authors":"Hanhan Xie, Fei Wang, Zidi He, Zhijian Liang, Jianguo Li, Minglei Zhao","doi":"10.1016/j.hpj.2024.02.010","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.02.010","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141986701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.1016/j.hpj.2023.12.013
Alessandra Gentile, Ilaria Inzirillo, Stefania Bennici, Francesco Scollo, Giuseppina Las Casas, Mario Di Guardo, Stefano La Malfa, Gaetano Distefano
Almond is widely cultivated in the world thanks to the quality and healthy features of the kernel. Almond kernel is consumed fresh or employed in the food industry. Hundreds of almond cultivars were selected throughout the long history of cultivation; in this context, an efficient method for varietal identification is essential to ensure cultivar traceability along the chain. This study surveyed the widely employed commercial kits and protocols for DNA extraction from several almond matrices including leaves, kernels (fresh and roasted) and several processed products. Commercial kits (though with minor modification) outperformed the other extraction methods for the isolation of DNA suitable for molecular analysis from all the tested matrices. In parallel, a germplasm collection composed of 140 accessions (123 Sicilian genotypes complemented with widely known national and international cultivars) was genotyped with the Axiom™ 60K almond SNP Array enabling the detection of 6 374 unique SNPs that can be readily used for varietal traceability. A subset of unique SNPs was further validated employing a high-resolution melting (HRM) assay on a discovery panel encompassing ten of the most widely cultivated accessions. The DNA extracted from leaves and kernels of five cultivars was genotyped with eight SSRs allowing the identification of the maternal origin of each kernel. The paper integrates the survey of the widely employed protocols for DNA extraction with the high-throughput genotyping of 140 almond accessions. In this context, unique SNPs validated and optimized for an HRM assay and the availability of SSR markers demonstrated their efficacy in traceability analysis along the chain.
杏仁因其优质和健康的特点而在世界各地广泛种植。杏仁可新鲜食用或用于食品工业。在漫长的栽培历史中，人们选择了数以百计的杏仁栽培品种；在这种情况下，一种有效的品种鉴定方法对于确保栽培品种在整个产业链中的可追溯性至关重要。本研究调查了从多种杏仁基质（包括杏仁叶、杏仁核（新鲜和烘焙）以及多种加工产品）中提取 DNA 的广泛使用的商业试剂盒和方案。在从所有测试基质中分离出适合分子分析的 DNA 方面，商业试剂盒（尽管略有改动）优于其他提取方法。与此同时，利用 Axiom™ 60K 杏仁 SNP 阵列对由 140 个登录品种（123 个西西里基因型，以及广为人知的国内和国际栽培品种）组成的种质集合进行了基因分型，从而检测出 6 374 个独特的 SNPs，这些 SNPs 可随时用于品种溯源。利用高分辨率熔解（HRM）测定法对包含十个最广泛栽培品种的发现面板上的独特 SNP 子集进行了进一步验证。从 5 个栽培品种的叶片和果核中提取的 DNA 通过 8 个 SSR 进行了基因分型，从而确定了每个果核的母本来源。论文将对广泛使用的 DNA 提取方案的调查与 140 个杏仁品种的高通量基因分型相结合。在此背景下，经过验证和优化的独特 SNPs 用于 HRM 检测，SSR 标记的可用性证明了它们在产业链溯源分析中的功效。
{"title":"Genomic approaches for almond traceability from nursery and along the food chain","authors":"Alessandra Gentile, Ilaria Inzirillo, Stefania Bennici, Francesco Scollo, Giuseppina Las Casas, Mario Di Guardo, Stefano La Malfa, Gaetano Distefano","doi":"10.1016/j.hpj.2023.12.013","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.12.013","url":null,"abstract":"Almond is widely cultivated in the world thanks to the quality and healthy features of the kernel. Almond kernel is consumed fresh or employed in the food industry. Hundreds of almond cultivars were selected throughout the long history of cultivation; in this context, an efficient method for varietal identification is essential to ensure cultivar traceability along the chain. This study surveyed the widely employed commercial kits and protocols for DNA extraction from several almond matrices including leaves, kernels (fresh and roasted) and several processed products. Commercial kits (though with minor modification) outperformed the other extraction methods for the isolation of DNA suitable for molecular analysis from all the tested matrices. In parallel, a germplasm collection composed of 140 accessions (123 Sicilian genotypes complemented with widely known national and international cultivars) was genotyped with the Axiom™ 60K almond SNP Array enabling the detection of 6 374 unique SNPs that can be readily used for varietal traceability. A subset of unique SNPs was further validated employing a high-resolution melting (HRM) assay on a discovery panel encompassing ten of the most widely cultivated accessions. The DNA extracted from leaves and kernels of five cultivars was genotyped with eight SSRs allowing the identification of the maternal origin of each kernel. The paper integrates the survey of the widely employed protocols for DNA extraction with the high-throughput genotyping of 140 almond accessions. In this context, unique SNPs validated and optimized for an HRM assay and the availability of SSR markers demonstrated their efficacy in traceability analysis along the chain.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141904801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-30DOI: 10.1016/j.hpj.2023.09.011
Jiahao Chen, Yang Hu, Zhangsheng Zhu, Peng Zheng, Shaoqun Liu, Binmei Sun
DNA methylation plays important roles in regulating gene expression during development. However, little is known about the influence of DNA methylation on secondary metabolism during leaf development in the tea plant (). In this study, we combined the methylome, transcriptome, and metabolome to investigate the dynamic changes in DNA methylation and its potential regulatory roles in secondary metabolite biosynthesis. In this study, the level of genomic DNA methylation increased as leaf development progressed from tender to old leaf. It additionally exhibited a similar distribution across the genomic background at the two distinct developmental stages studied. Notably, integrated analysis of transcriptomic and methylomic data showed that DNA hypermethylation primarily occurred in genes of the phenylpropanoid, flavonoid, and terpenoid biosynthesis pathways. The effect of methylation on transcription of these secondary metabolite biosynthesis genes was dependent on the location of methylation (i.e., in the promoter, gene or intergenic regions) and the sequence context (i.e., CpG, CHG, or CHH). Changes in the content of catechins and terpenoids were consistent with the changes in gene transcription and the methylation state of structural genes, such as (), (), and (). Our study provides valuable information for dissecting the effects of DNA methylation on regulation of genes involved in secondary metabolism during tea leaf development.
DNA 甲基化在调节发育过程中的基因表达方面发挥着重要作用。然而，人们对茶树叶片发育过程中DNA甲基化对次生代谢的影响知之甚少（）。在本研究中，我们结合甲基组、转录组和代谢组来研究DNA甲基化的动态变化及其在次生代谢物生物合成中的潜在调控作用。在这项研究中，基因组 DNA 甲基化水平随着叶片从嫩叶到老叶的发育过程而增加。此外，在所研究的两个不同的发育阶段，基因组背景中的甲基化也呈现出相似的分布。值得注意的是，对转录组和甲基组数据的综合分析表明，DNA高甲基化主要发生在苯丙类、黄酮类和萜类生物合成途径的基因中。甲基化对这些次生代谢物生物合成基因转录的影响取决于甲基化的位置（即启动子、基因或基因间区）和序列上下文（即 CpG、CHG 或 CHH）。儿茶素和萜类化合物含量的变化与（）、（）和（）等结构基因的基因转录和甲基化状态的变化一致。我们的研究为剖析 DNA 甲基化对茶叶发育过程中参与次生代谢的基因的调控作用提供了有价值的信息。
{"title":"Dynamic DNA methylation modification in catechins and terpenoids biosynthesis during tea plant (Camellia sinensis) leaf development","authors":"Jiahao Chen, Yang Hu, Zhangsheng Zhu, Peng Zheng, Shaoqun Liu, Binmei Sun","doi":"10.1016/j.hpj.2023.09.011","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.09.011","url":null,"abstract":"DNA methylation plays important roles in regulating gene expression during development. However, little is known about the influence of DNA methylation on secondary metabolism during leaf development in the tea plant (). In this study, we combined the methylome, transcriptome, and metabolome to investigate the dynamic changes in DNA methylation and its potential regulatory roles in secondary metabolite biosynthesis. In this study, the level of genomic DNA methylation increased as leaf development progressed from tender to old leaf. It additionally exhibited a similar distribution across the genomic background at the two distinct developmental stages studied. Notably, integrated analysis of transcriptomic and methylomic data showed that DNA hypermethylation primarily occurred in genes of the phenylpropanoid, flavonoid, and terpenoid biosynthesis pathways. The effect of methylation on transcription of these secondary metabolite biosynthesis genes was dependent on the location of methylation (i.e., in the promoter, gene or intergenic regions) and the sequence context (i.e., CpG, CHG, or CHH). Changes in the content of catechins and terpenoids were consistent with the changes in gene transcription and the methylation state of structural genes, such as (), (), and (). Our study provides valuable information for dissecting the effects of DNA methylation on regulation of genes involved in secondary metabolism during tea leaf development.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141904800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The essential photoprotective role of proton gradient regulation 5 (PGR5)-dependent cyclic electron flow (CEF) has been reported in , rice, and algae. However, its functional assessment has not been performed in tomato yet. In this study, we focused on elucidate the function of and in tomato. We performed RNA interference and found that -suppressed transformants exhibited extremely low CO assimilation capacity, their photosystem I (PSI) and PSII were severely photoinhibited and chloroplasts were obviously damaged. The -suppressed plants almost completely inhibited CEF and Y(ND), and PSII photoinhibition may be directly related to the inability to produce sufficient proton motive force to induce NPQ. The transgenic plants overexpressing and driven by 35S promoter capable alleviate photoinhibition of plants under low night temperature. The transcriptomic and proteomic analyses suggested that the nuclear gene transcription and turnover of chloroplast proteins, including the plastoglobule-related proteins, were closely related to SlPGR5/SlPGRL1 pathway dependent CEF. The bridge relationship between CEF and chloroplast quality maintenance was a novel report to our knowledge. In conclusion, these results revealed the regulatory mechanism of the SlPGR5/SlPGRL1 pathway in photoprotection and maintenance of chloroplast function in tomato, which is crucial for reduce yield loss, especially under adverse environmental conditions.
{"title":"SlPGR5/SlPGRL1 pathway-dependent cyclic electron transport regulates photoprotection and chloroplast quality in tomato plants","authors":"Xiaolong Yang, Yumeng Zhang, Ting Liu, Jiali Shi, Mingfang Qi, Riyuan Chen, Yufeng Liu, Tianlai Li","doi":"10.1016/j.hpj.2023.06.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.06.009","url":null,"abstract":"The essential photoprotective role of proton gradient regulation 5 (PGR5)-dependent cyclic electron flow (CEF) has been reported in , rice, and algae. However, its functional assessment has not been performed in tomato yet. In this study, we focused on elucidate the function of and in tomato. We performed RNA interference and found that -suppressed transformants exhibited extremely low CO assimilation capacity, their photosystem I (PSI) and PSII were severely photoinhibited and chloroplasts were obviously damaged. The -suppressed plants almost completely inhibited CEF and Y(ND), and PSII photoinhibition may be directly related to the inability to produce sufficient proton motive force to induce NPQ. The transgenic plants overexpressing and driven by 35S promoter capable alleviate photoinhibition of plants under low night temperature. The transcriptomic and proteomic analyses suggested that the nuclear gene transcription and turnover of chloroplast proteins, including the plastoglobule-related proteins, were closely related to SlPGR5/SlPGRL1 pathway dependent CEF. The bridge relationship between CEF and chloroplast quality maintenance was a novel report to our knowledge. In conclusion, these results revealed the regulatory mechanism of the SlPGR5/SlPGRL1 pathway in photoprotection and maintenance of chloroplast function in tomato, which is crucial for reduce yield loss, especially under adverse environmental conditions.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141904802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-20DOI: 10.1016/j.hpj.2023.12.012
Ran Yan, Mariama Kebbeh, Yuan Cheng, Yue Wang, Liu Yan, Chen Huan, Xiaolin Zheng, Shuling Shen
Yellowing of broccoli is a crucial limiting factor for its commercial value and consumer acceptance during postharvest. In this study, the impacts of exogenous melatonin (MEL) on chlorophyll content and fluorescence, as well as ultrastructure and membrane lipid metabolism of chloroplasts in broccoli were investigated during postharvest. The results showed that MEL treatment (200 μmol L) maintained the chlorophyll content, chloroplast autofluorescence and integral structure, and reduced the level ofserotonin in the chloroplasts in broccoli. Also, MEL treatment inhibited the membrane lipid peroxidation of chloroplasts, as indicated by low levels of superoxide anion (O), hydrogen peroxide (HO) and malondialdehyde (MDA), and high levels of endogenous MEL. In addition, the stability and fluidity of chloroplast membranes were also better maintained in the treated broccoli via increasing the contents of phosphatidylglyceroland (PG), monogalactosyldiglyceride (MGDG), digalactosyldiglyceride (DGDG) and unsaturated fatty acids as well as decreasing saturated fatty acid content and the activities of lipoxygenase (LOX) and lipase (LPS). Thus, the application of MEL facilitated the maintenance of chloroplast integrity, thus contributing to yellowing postponement and the extension of the storage life of broccoli.
{"title":"Exogenous melatonin delays yellowing in harvested broccoli by maintaining chloroplast integrity","authors":"Ran Yan, Mariama Kebbeh, Yuan Cheng, Yue Wang, Liu Yan, Chen Huan, Xiaolin Zheng, Shuling Shen","doi":"10.1016/j.hpj.2023.12.012","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.12.012","url":null,"abstract":"Yellowing of broccoli is a crucial limiting factor for its commercial value and consumer acceptance during postharvest. In this study, the impacts of exogenous melatonin (MEL) on chlorophyll content and fluorescence, as well as ultrastructure and membrane lipid metabolism of chloroplasts in broccoli were investigated during postharvest. The results showed that MEL treatment (200 μmol L) maintained the chlorophyll content, chloroplast autofluorescence and integral structure, and reduced the level ofserotonin in the chloroplasts in broccoli. Also, MEL treatment inhibited the membrane lipid peroxidation of chloroplasts, as indicated by low levels of superoxide anion (O), hydrogen peroxide (HO) and malondialdehyde (MDA), and high levels of endogenous MEL. In addition, the stability and fluidity of chloroplast membranes were also better maintained in the treated broccoli via increasing the contents of phosphatidylglyceroland (PG), monogalactosyldiglyceride (MGDG), digalactosyldiglyceride (DGDG) and unsaturated fatty acids as well as decreasing saturated fatty acid content and the activities of lipoxygenase (LOX) and lipase (LPS). Thus, the application of MEL facilitated the maintenance of chloroplast integrity, thus contributing to yellowing postponement and the extension of the storage life of broccoli.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141768945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-19DOI: 10.1016/j.hpj.2024.02.009
Hang Yu, Min Gao, Chunce Guo, Haifeng Wang
Moso bamboo holds significant economic importance in China, serving various purposes, such as food, material, ornamentation, and greenery. Despite its versatility, the occurrence of flowering in Moso bamboo poses a threat to bamboo forests, resulting in substantial losses. The underlying cause of bamboo flowering remains elusive. Dynamic fluctuations in DNA methylation govern the transcriptional levels of crucial genes pivotal for plant growth and development. In this study, we conducted comprehensive DNA methylation (by whole-genome bisulfite sequencing) and transcriptome (by RNA-seq) analyses on non-flowering leaves, flowering leaves, and spikelets of Moso bamboo. Our findings revealed a notable reduction in the overall DNA methylation level, particularly CHH methylation, from leaves to spikelets, influencing the expression of differentially regulated genes. Notably, we identified DNA methylation as a regulatory mechanism for numerous flowering-related genes, including , , and . Specifically, the gene, a key regulator of the aging pathway, exhibited hypomethylation and a high expression level in spikelets. Conversely, displayed transcriptional silencing attributed to hypermethylation in the CHH context in the leaves of non-flowering plants. DNA methylation may affect the flowering mechanism of Moso bamboo by regulating the expression of key genes. In summary, our results shed light on the dynamic changes in DNA methylation between leaves and spikelets, unraveling an important epigenetic modification mechanism for flowering in Moso bamboo.
毛竹在中国具有重要的经济价值，可用于食品、材料、装饰和绿化等多种用途。尽管毛竹用途广泛，但毛竹开花对竹林构成威胁，造成巨大损失。竹子开花的根本原因仍然难以捉摸。DNA 甲基化的动态波动控制着植物生长和发育关键基因的转录水平。在这项研究中，我们对毛竹的非开花叶片、开花叶片和小穗进行了全面的 DNA 甲基化（通过全基因组亚硫酸氢盐测序）和转录组（通过 RNA-seq）分析。我们的研究结果表明，从叶片到小穗，整体DNA甲基化水平显著降低，尤其是CHH甲基化，影响了不同调控基因的表达。值得注意的是，我们发现DNA甲基化是许多开花相关基因的调控机制，包括 、 和 。具体来说，该基因是衰老途径的关键调控因子，在小穗中表现出低甲基化和高表达水平。相反，在非开花植物的叶片中，由于 CHH 上下文的高甲基化，显示了转录沉默。DNA甲基化可能通过调节关键基因的表达来影响毛竹的开花机制。总之，我们的研究结果揭示了叶片和小穗之间DNA甲基化的动态变化，揭示了毛竹开花的重要表观遗传修饰机制。
{"title":"Reduced CHH methylation levels reveal a critical role of aging pathway genes in Moso bamboo flowering","authors":"Hang Yu, Min Gao, Chunce Guo, Haifeng Wang","doi":"10.1016/j.hpj.2024.02.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.02.009","url":null,"abstract":"Moso bamboo holds significant economic importance in China, serving various purposes, such as food, material, ornamentation, and greenery. Despite its versatility, the occurrence of flowering in Moso bamboo poses a threat to bamboo forests, resulting in substantial losses. The underlying cause of bamboo flowering remains elusive. Dynamic fluctuations in DNA methylation govern the transcriptional levels of crucial genes pivotal for plant growth and development. In this study, we conducted comprehensive DNA methylation (by whole-genome bisulfite sequencing) and transcriptome (by RNA-seq) analyses on non-flowering leaves, flowering leaves, and spikelets of Moso bamboo. Our findings revealed a notable reduction in the overall DNA methylation level, particularly CHH methylation, from leaves to spikelets, influencing the expression of differentially regulated genes. Notably, we identified DNA methylation as a regulatory mechanism for numerous flowering-related genes, including , , and . Specifically, the gene, a key regulator of the aging pathway, exhibited hypomethylation and a high expression level in spikelets. Conversely, displayed transcriptional silencing attributed to hypermethylation in the CHH context in the leaves of non-flowering plants. DNA methylation may affect the flowering mechanism of Moso bamboo by regulating the expression of key genes. In summary, our results shed light on the dynamic changes in DNA methylation between leaves and spikelets, unraveling an important epigenetic modification mechanism for flowering in Moso bamboo.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141768946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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