Pub Date : 2024-05-31DOI: 10.1016/j.hpj.2023.12.009
Guoliang Ma, Yunyun Han, Tongtong Li, Tao Xia, Liping Gao
The members of the fourth subgroup of R2R3-MYB (Sg4 members) are well-known inhibitors of phenylpropanoid and lignin synthesis pathways. The C2 domain is closely related to the transcriptional inhibitory activity of Sg4 members. Phosphorylation modification enhances the transcriptional inhibitory activity of Sg4 members. Here, we identified a phosphorylation site on the C2 domain of CsMYB4a from tea plants (). A mitogen-activated protein kinase (MAPK), named CsMPK3-2, phosphorylated this site on the C2 domain of CsMYB4a. Further experiments revealed that phosphorylation of CsMYB4a weakened its ability to inhibit the gene expression of , , and 4CL in the phenylpropanoid pathway and activated the expression of transcription factor , maintaining the adaxial–abaxial polarity of the leaf. Knocking out in transgenic tobacco partially repaired the leaf wrinkling phenotype caused by . The C1 domain exhibited an activation function when the C2 domain of CsMYB4a was phosphorylated by CsMPK3-2, causing this reversal phenomenon. These results enrich our understanding of the regulatory diversity of Sg4 members.
{"title":"Phosphorylation modification reverses the transcriptional inhibitory activity of CsMYB4a in tea plants (Camellia sinensis)","authors":"Guoliang Ma, Yunyun Han, Tongtong Li, Tao Xia, Liping Gao","doi":"10.1016/j.hpj.2023.12.009","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.12.009","url":null,"abstract":"The members of the fourth subgroup of R2R3-MYB (Sg4 members) are well-known inhibitors of phenylpropanoid and lignin synthesis pathways. The C2 domain is closely related to the transcriptional inhibitory activity of Sg4 members. Phosphorylation modification enhances the transcriptional inhibitory activity of Sg4 members. Here, we identified a phosphorylation site on the C2 domain of CsMYB4a from tea plants (). A mitogen-activated protein kinase (MAPK), named CsMPK3-2, phosphorylated this site on the C2 domain of CsMYB4a. Further experiments revealed that phosphorylation of CsMYB4a weakened its ability to inhibit the gene expression of , , and 4CL in the phenylpropanoid pathway and activated the expression of transcription factor , maintaining the adaxial–abaxial polarity of the leaf. Knocking out in transgenic tobacco partially repaired the leaf wrinkling phenotype caused by . The C1 domain exhibited an activation function when the C2 domain of CsMYB4a was phosphorylated by CsMPK3-2, causing this reversal phenomenon. These results enrich our understanding of the regulatory diversity of Sg4 members.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chlorophyll degradation and carotenoid accumulation are essential processes of fruit maturation in many horticultural plants, and play a crucial role in fruit color and quality. The pathways of chlorophyll and carotenoid biosynthesis and degradation are well understood, and key regulatory genes controlling these pathways have been identified in citrus. This article reviewed the recent research on chlorophyll and carotenoid metabolism in citrus fruits, encompassing the metabolic pathways, transcriptional regulation, influencing factors, and the interplay between chlorophyll and carotenoid metabolism, aiming to provide insights into the molecular regulatory mechanisms governing the coloration of citrus fruits.
{"title":"Regulation of chlorophyll and carotenoid metabolism in citrus fruit","authors":"Yingxiao Lu, Xinchen Shen, Yuche Li, Yanna Xu, Yuhan Chen, Yishan Chen, Xiaoli Hu, Xiaolong Li, Xuepeng Sun, Jinli Gong","doi":"10.1016/j.hpj.2024.02.004","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.02.004","url":null,"abstract":"Chlorophyll degradation and carotenoid accumulation are essential processes of fruit maturation in many horticultural plants, and play a crucial role in fruit color and quality. The pathways of chlorophyll and carotenoid biosynthesis and degradation are well understood, and key regulatory genes controlling these pathways have been identified in citrus. This article reviewed the recent research on chlorophyll and carotenoid metabolism in citrus fruits, encompassing the metabolic pathways, transcriptional regulation, influencing factors, and the interplay between chlorophyll and carotenoid metabolism, aiming to provide insights into the molecular regulatory mechanisms governing the coloration of citrus fruits.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-31DOI: 10.1016/j.hpj.2024.03.003
Manisha Mahanta, Saikat Gantait
Gerbera, a popular commercial cut flower with vibrant and striking colours has gained immense popularity in the floriculture industry. They are widely cultivated in various regions, making them available throughout the year. As a better alternative to conventional propagation methods (via seeds and rhizomes), plant tissue culture serves as way to avail large-scale, uniform, disease-free plantlets for commercial cultivation as well as to develop novel genotypes. In addition, it ensures production of healthy plantlets throughout the year in limited space. Based on the plant tissue culture techniques, the polyploidization, mutagenesis and genetic transformation pave a path for creation of variation and eventually enhancing the ornamental traits to address the consumers’ preferences and also facilitates in developing stress tolerant lines thereby minimising the losses during cultivation maintaining the quality of the flowers. This comprehensive review article presents an overview of the recent advancements on genetic improvement of gerbera via various cutting-edge plant tissue culture-based tools and techniques that contribute in enhancing the quality and efficiency of gerbera cultivation, meeting the demands of the floriculture industry while addressing the challenges of changing environment and resource limitations.
{"title":"Trends in plant tissue culture and genetic improvement of gerbera","authors":"Manisha Mahanta, Saikat Gantait","doi":"10.1016/j.hpj.2024.03.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.03.003","url":null,"abstract":"Gerbera, a popular commercial cut flower with vibrant and striking colours has gained immense popularity in the floriculture industry. They are widely cultivated in various regions, making them available throughout the year. As a better alternative to conventional propagation methods (via seeds and rhizomes), plant tissue culture serves as way to avail large-scale, uniform, disease-free plantlets for commercial cultivation as well as to develop novel genotypes. In addition, it ensures production of healthy plantlets throughout the year in limited space. Based on the plant tissue culture techniques, the polyploidization, mutagenesis and genetic transformation pave a path for creation of variation and eventually enhancing the ornamental traits to address the consumers’ preferences and also facilitates in developing stress tolerant lines thereby minimising the losses during cultivation maintaining the quality of the flowers. This comprehensive review article presents an overview of the recent advancements on genetic improvement of gerbera via various cutting-edge plant tissue culture-based tools and techniques that contribute in enhancing the quality and efficiency of gerbera cultivation, meeting the demands of the floriculture industry while addressing the challenges of changing environment and resource limitations.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Grapevine ( sp.) is one of the most important economic fruit crops all over the world, and the formation of adventitious roots (ARs) is crucial for the vegetative reproduction of grapes. However, studies on the regulatory mechanisms of this process are currently lacking. In this study, we applied an efficient and convenient leave-petiole (LP) system for studying ARs, revealing a significant inhibition of root primordia formation under continuous-light treatment. The results showed that isolated ARs of grapevine were induced and originated from ray cells near the vascular cambium, with the process categorized into induction, initiation, and extension stages. LP samples under light and dark conditions were used for transcriptome sequencing and endogenous hormone measurements at three critical time points of AR formation. A total of 37 155 transcripts were obtained, and 7041 genes showed significantly different expression levels in the petiole. An integrated analysis, including Gene Ontology (GO) enrichment analysis, weighted gene co-expression network analysis (WGCNA), and hormonal content determination, showed that several genes (, , , , , , , etc.) associated with hormone signals, sugar synthesis and transport, reactive oxygen species (ROS) scavenging, cell wall biogenesis, flavonoid biosynthesis, microtubule remodeling, and some transcription factors (HY5, COP1, ERF2, MYB15, etc) played vital roles in light-induced AR formation. A hypothetical model was initially constructed, which illustrated the centrality of auxin in HY5-dependent AR formation and the complex crosstalk among various factors. The results of this study provided abundant genetic resources and a novel perspective for understanding the molecular mechanisms of AR formation in grapevine.
葡萄是全世界最重要的经济果树作物之一，不定根（ARs）的形成对葡萄的无性繁殖至关重要。然而，目前还缺乏对这一过程的调控机制的研究。在本研究中，我们采用了一种高效便捷的叶柄（LP）系统来研究不定根，结果发现在连续光照处理下，不定根的初生根形成受到了明显的抑制。结果表明，分离出的葡萄ARs是诱导的，起源于维管束韧皮部附近的射线细胞，其过程可分为诱导期、起始期和延伸期。在AR形成的三个关键时间点，对光照和黑暗条件下的LP样本进行了转录组测序和内源激素测定。共获得 37 155 个转录本，其中 7041 个基因在叶柄中的表达水平有显著差异。包括基因本体（GO）富集分析、加权基因共表达网络分析（WGCNA）和激素含量测定在内的综合分析表明，多个基因（、、、、、等）与激素信号、内源激素和植物生长有关。与激素信号、糖的合成和运输、活性氧（ROS）清除、细胞壁生物生成、类黄酮生物合成、微管重塑相关的基因以及一些转录因子（HY5、COP1、ERF2、MYB15 等）在光诱导 AR 的形成过程中发挥了重要作用。研究初步构建了一个假说模型，说明了辅助素在 HY5 依赖性 AR 形成过程中的核心作用以及各种因素之间复杂的相互影响。该研究结果为了解葡萄AR形成的分子机制提供了丰富的遗传资源和新的视角。
{"title":"Comparative transcriptome profiling reveals that light coordinates auxin to inhibit adventitious root formation in grapevine","authors":"Yunzhang Yuan, Miao Bai, Peiyi Ni, Yanxia Li, Xinyu Chang, Jingjing He, Guoshun Yang, Shuangjiang Li","doi":"10.1016/j.hpj.2024.02.003","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.02.003","url":null,"abstract":"Grapevine ( sp.) is one of the most important economic fruit crops all over the world, and the formation of adventitious roots (ARs) is crucial for the vegetative reproduction of grapes. However, studies on the regulatory mechanisms of this process are currently lacking. In this study, we applied an efficient and convenient leave-petiole (LP) system for studying ARs, revealing a significant inhibition of root primordia formation under continuous-light treatment. The results showed that isolated ARs of grapevine were induced and originated from ray cells near the vascular cambium, with the process categorized into induction, initiation, and extension stages. LP samples under light and dark conditions were used for transcriptome sequencing and endogenous hormone measurements at three critical time points of AR formation. A total of 37 155 transcripts were obtained, and 7041 genes showed significantly different expression levels in the petiole. An integrated analysis, including Gene Ontology (GO) enrichment analysis, weighted gene co-expression network analysis (WGCNA), and hormonal content determination, showed that several genes (, , , , , , , etc.) associated with hormone signals, sugar synthesis and transport, reactive oxygen species (ROS) scavenging, cell wall biogenesis, flavonoid biosynthesis, microtubule remodeling, and some transcription factors (HY5, COP1, ERF2, MYB15, etc) played vital roles in light-induced AR formation. A hypothetical model was initially constructed, which illustrated the centrality of auxin in HY5-dependent AR formation and the complex crosstalk among various factors. The results of this study provided abundant genetic resources and a novel perspective for understanding the molecular mechanisms of AR formation in grapevine.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141430592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fruit ripening, which is modulated by the up- and downregulation of numerous genes, is a sophisticated physiological event determining consumer acceptability. While many positive regulators have been known to regulate fruit ripening, relatively less information is associated with the negative regulators in the process. Here, a negative regulator, , was characterized according to the banana fruit ripening transcriptome, which displayed nuclear localization and inhibitory transactivation activity. MaMADS31 suppresses the transcription of the cell wall modification gene and the ethylene biosynthesis-related gene 3 by directly recognizing the CArG-box element in their promoters. Transient expression of in banana fruit brought about downregulation of and , thereby delaying fruit ripening. Importantly, MaMADS31 interacts with MaBZR2 to synergistically strengthen the transcriptional inhibition of and . Overall, MaMADS31-MaBZR2 plays a negative role in fruit ripening by downregulating the and transcription, which provides new insights for innovating approaches for prolonging the postharvest life of horticultural plants.
{"title":"A novel module of MaMADS31-MaBZR2 confers negative regulation of banana fruit ripening","authors":"Chaojie Wu, Wei Wei, Danling Cai, Zengxiang Lin, Yingying Yang, Jia Si, Wei Shan, Jianye Chen, Wangjin Lu, Jianfei Kuang","doi":"10.1016/j.hpj.2023.12.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.12.008","url":null,"abstract":"Fruit ripening, which is modulated by the up- and downregulation of numerous genes, is a sophisticated physiological event determining consumer acceptability. While many positive regulators have been known to regulate fruit ripening, relatively less information is associated with the negative regulators in the process. Here, a negative regulator, , was characterized according to the banana fruit ripening transcriptome, which displayed nuclear localization and inhibitory transactivation activity. MaMADS31 suppresses the transcription of the cell wall modification gene and the ethylene biosynthesis-related gene 3 by directly recognizing the CArG-box element in their promoters. Transient expression of in banana fruit brought about downregulation of and , thereby delaying fruit ripening. Importantly, MaMADS31 interacts with MaBZR2 to synergistically strengthen the transcriptional inhibition of and . Overall, MaMADS31-MaBZR2 plays a negative role in fruit ripening by downregulating the and transcription, which provides new insights for innovating approaches for prolonging the postharvest life of horticultural plants.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141246580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fig fruit firmness decreases rapidly during ripening and after harvest, resulting in poor storability and transportation loss, which severely restricts development of the fresh fig industry. APETALA2/ethylene-responsive factor (AP2/ERF) transcription factors are downstream components of the ethylene-signaling pathway that play crucial roles in quality formation during fruit ripening. In this study, (Fc) ERF12 was clustered in repressor subfamily VIII of ERFs through phylogenetic analysis, and further recruited by its two EAR motifs and expression pattern during fig ripening. DNA affinity purification sequencing analysis indicated that FcERF12 binds to the promoter or gene body regions of multiple ripening-related genes, including cell wall-modification genes , and , and ethylene-biosynthesis genes and . Yeast two-hybrid assay demonstrated that FcERF12 interacts with TOPLESS (TPL) co-repressors FcTPL1, FcTPL4 and FcTPL5, and histone deacetylases FcHDA6 and FcHDA19; interaction with FcTPL4 and FcTPL5 relied on the C-terminal EAR motif. Overexpressing in tomato did not change fruit size or yield, but resulted in an 18.37% increment in fruit firmness and a 49.62% reduction in ethylene-release rate at fruit ripening, accompanied by a significant decrease in seed number per fruit. Transcriptomic analysis revealed downregulation of tomato cell wall-modification genes , and , and ethylene-synthesis genes and . Metabolomic profiling identified 82 differentially accumulated flavonoid metabolites, 61 of them showing significantly decreased contents. Taken together, our results exhibit the negative regulatory role of FcERF12 in fig ethylene-signal transduction, providing new information on precise control of fruit firmness and other quality traits at ripening.
{"title":"Ficus carica ERF12 improves fruit firmness at ripening","authors":"Yanlei Zhai, Yuanyuan Cui, Zhiyi Fan, Hantang Huang, Zhe Wang, Shangwu Chen, Huiqin Ma","doi":"10.1016/j.hpj.2024.03.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2024.03.002","url":null,"abstract":"Fig fruit firmness decreases rapidly during ripening and after harvest, resulting in poor storability and transportation loss, which severely restricts development of the fresh fig industry. APETALA2/ethylene-responsive factor (AP2/ERF) transcription factors are downstream components of the ethylene-signaling pathway that play crucial roles in quality formation during fruit ripening. In this study, (Fc) ERF12 was clustered in repressor subfamily VIII of ERFs through phylogenetic analysis, and further recruited by its two EAR motifs and expression pattern during fig ripening. DNA affinity purification sequencing analysis indicated that FcERF12 binds to the promoter or gene body regions of multiple ripening-related genes, including cell wall-modification genes , and , and ethylene-biosynthesis genes and . Yeast two-hybrid assay demonstrated that FcERF12 interacts with TOPLESS (TPL) co-repressors FcTPL1, FcTPL4 and FcTPL5, and histone deacetylases FcHDA6 and FcHDA19; interaction with FcTPL4 and FcTPL5 relied on the C-terminal EAR motif. Overexpressing in tomato did not change fruit size or yield, but resulted in an 18.37% increment in fruit firmness and a 49.62% reduction in ethylene-release rate at fruit ripening, accompanied by a significant decrease in seed number per fruit. Transcriptomic analysis revealed downregulation of tomato cell wall-modification genes , and , and ethylene-synthesis genes and . Metabolomic profiling identified 82 differentially accumulated flavonoid metabolites, 61 of them showing significantly decreased contents. Taken together, our results exhibit the negative regulatory role of FcERF12 in fig ethylene-signal transduction, providing new information on precise control of fruit firmness and other quality traits at ripening.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141246672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-01DOI: 10.1016/j.hpj.2023.09.008
Que Wang, Yaqiong Wu, Wenlong Wu, L. Lyu, Weilin Li
{"title":"Research progress on the physiological, biochemical and molecular regulatory mechanisms of fruit tree responses to high-temperature stress","authors":"Que Wang, Yaqiong Wu, Wenlong Wu, L. Lyu, Weilin Li","doi":"10.1016/j.hpj.2023.09.008","DOIUrl":"https://doi.org/10.1016/j.hpj.2023.09.008","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":null,"pages":null},"PeriodicalIF":5.7,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141031671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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