Pub Date : 2026-01-23DOI: 10.1016/j.hpj.2025.09.012
Yujie Yang, Xiaowei Li, Ming Sun, Man Zhang, Tangren Cheng, Cunquan Yuan, Qixiang Zhang
{"title":"Construction of an interspecific genetic map and multiyear phenotype QTL mapping of the pentaploid Chrysanthemum","authors":"Yujie Yang, Xiaowei Li, Ming Sun, Man Zhang, Tangren Cheng, Cunquan Yuan, Qixiang Zhang","doi":"10.1016/j.hpj.2025.09.012","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.09.012","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"38 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146033224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-21DOI: 10.1016/j.hpj.2025.07.026
Min He, Lei Wu, Yipeng Ye, Kaijie Qi, Chao Gu, Shaoling Zhang
{"title":"Genetic and Functional Analysis of S-alleles in Chinese Pear and the self-compatible mutant ‘Zaoguan’","authors":"Min He, Lei Wu, Yipeng Ye, Kaijie Qi, Chao Gu, Shaoling Zhang","doi":"10.1016/j.hpj.2025.07.026","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.07.026","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"92 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146014558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Red-fleshed fruits are valued for their vibrant color and high anthocyanin content. Pre-harvest fruit bagging enhances fruit peel pigmentation, but its effect on flesh coloration remains poorly characterized. This study revealed that removing bags from ‘Gengcunyangtao’ red-fleshed peach fruits triggers the rapid and uniform accumulation of anthocyanins in the flesh, resulting in anthocyanin levels that exceed those in unbagged fruits. The exposure to light after bag removal triggered significant increases in anthocyanin levels within 24 h. This was accompanied by the rapid upregulation of light-responsive and flavonoid biosynthetic gene expression levels within 6 h. A metabolomic analysis indicated that anthocyanin precursors, especially p-coumaric acid, accumulated before bag removal, thereby increasing substrate availability for rapid anthocyanin synthesis. On the basis of a weighted gene co-expression network analysis, MYB transcription factors, anthocyanin transporters, glutathione S-transferase, and multidrug and toxic compound extrusion (MATE) were identified as key regulators that coordinate precursor storage along with light-induced transcriptional activation. Notably, PpMYB4 binds to the promoter of PpGSTF14 and activates its expression, thereby promoting anthocyanin accumulation. The study findings elucidated the temporal coordination of metabolic priming and light-responsive transcriptional regulation driving rapid anthocyanin biosynthesis, with possible implications for improving peach fruit flesh coloration.
{"title":"Integrated transcriptome and metabolome analysis reveals light induction of anthocyanin rapid accumulation in red-fleshed peach after pre-harvest bagging","authors":"Jia Wei, Fengjie He, Kexin Sun, Li Wang, Yiming Yin, Junbei Ni, Songling Bai, Yuanwen Teng, Huijuan Jia","doi":"10.1016/j.hpj.2025.11.002","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.11.002","url":null,"abstract":"Red-fleshed fruits are valued for their vibrant color and high anthocyanin content. Pre-harvest fruit bagging enhances fruit peel pigmentation, but its effect on flesh coloration remains poorly characterized. This study revealed that removing bags from ‘Gengcunyangtao’ red-fleshed peach fruits triggers the rapid and uniform accumulation of anthocyanins in the flesh, resulting in anthocyanin levels that exceed those in unbagged fruits. The exposure to light after bag removal triggered significant increases in anthocyanin levels within 24 h. This was accompanied by the rapid upregulation of light-responsive and flavonoid biosynthetic gene expression levels within 6 h. A metabolomic analysis indicated that anthocyanin precursors, especially <ce:italic>p</ce:italic>-coumaric acid, accumulated before bag removal, thereby increasing substrate availability for rapid anthocyanin synthesis. On the basis of a weighted gene co-expression network analysis, MYB transcription factors, anthocyanin transporters, glutathione <ce:italic>S</ce:italic>-transferase, and multidrug and toxic compound extrusion (MATE) were identified as key regulators that coordinate precursor storage along with light-induced transcriptional activation. Notably, PpMYB4 binds to the promoter of <ce:italic>PpGSTF14</ce:italic> and activates its expression, thereby promoting anthocyanin accumulation. The study findings elucidated the temporal coordination of metabolic priming and light-responsive transcriptional regulation driving rapid anthocyanin biosynthesis, with possible implications for improving peach fruit flesh coloration.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"11 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145956876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-09DOI: 10.1016/j.hpj.2025.08.019
Chunyu Shang, Guo Chen, Xianjue Ruan, Xiaoyan Liu, Dan Du, Xin Hu, Zhenglun Li, Qingyuan Li, Xinchen Niu, Dongdong Gao, Silong Li, Abid Khan, Jinhua Li, Yu Pan, Xiaohui Hu
Saline–alkali stress is a significant abiotic stressor that affects the growth of tomato (Solanum lycopersicum L.). Our previous studies reported that the homologous genes SlWRKY80 and SlWRKY81 can both resist saline-alkali stress by mediating the JA pathway. In this study, the co-overexpressing (SOE) and co-silencing (SQ) lines of SlWRKY80 and SlWRKY81 to saline-alkali treatment, and the results showed that SOE line had significantly higher morphological indices, physiological indices, and endogenous JA and MeJA contents than the WT, while SQ line was opposite. In addition, the SOE line showed a significant increase in autophagy against saline-alkali stress, and both SlWRKY80 and SlWRKY81 were positively regulating the transcription of the autophagy gene SlATG13b as screened and verified by transcriptome screening, qRT-PCR, WB (Western Blot), Y1H (Yeast One-Hybrid), ChIP-qPCR, EMSA (Electrophoretic Mobility Shift Assay), and D-LUC (D-Luciferin Experiment), and also had a synergistic effect in activating SlATG13b. The growth and physiological indexes of VIGS-SlATG13b line were significantly lower than those of other groups, and pro-SlATG13b is significantly activated by MeJA and saline-alkali signals, so the SlATG13b was regulating the resistance of tomato to saline-alkali stress. In summary, the increase of endogenous JA and MeJA content in tomato after saline-alkali stress inhibited the expression and synthesis of SlJAZ1, activated the transcription and protein synthesis of SlWRKY80 and SlWRKY81, and SlWRKY80 and SlWRKY81 coordinately regulated the transcription of SlATG13b, which resulted in the production of more autophagosomes and enhanced the resistance of tomato to saline-alkali stress. This study further improved the molecular mechanism of SlWRKY80 and SlWRKY81 mediating the JA pathway against saline-alkali stress, and laid a solid theoretical foundation for tomato saline-alkali resistance breeding.
{"title":"SlWRKY80 and SlWRKY81 synergistically regulate the autophagy gene SlATG13b to actively resist saline-alkali stress in tomato","authors":"Chunyu Shang, Guo Chen, Xianjue Ruan, Xiaoyan Liu, Dan Du, Xin Hu, Zhenglun Li, Qingyuan Li, Xinchen Niu, Dongdong Gao, Silong Li, Abid Khan, Jinhua Li, Yu Pan, Xiaohui Hu","doi":"10.1016/j.hpj.2025.08.019","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.08.019","url":null,"abstract":"Saline–alkali stress is a significant abiotic stressor that affects the growth of tomato (<ce:italic>Solanum lycopersicum</ce:italic> L.). Our previous studies reported that the homologous genes <ce:italic>SlWRKY80 and SlWRKY81</ce:italic> can both resist saline-alkali stress by mediating the JA pathway. In this study, the co-overexpressing (SOE) and co-silencing (SQ) lines of <ce:italic>SlWRKY80 and SlWRKY81</ce:italic> to saline-alkali treatment, and the results showed that SOE line had significantly higher morphological indices, physiological indices, and endogenous JA and MeJA contents than the WT, while SQ line was opposite. In addition, the SOE line showed a significant increase in autophagy against saline-alkali stress, and both SlWRKY80 and SlWRKY81 were positively regulating the transcription of the autophagy gene <ce:italic>SlATG13b</ce:italic> as screened and verified by transcriptome screening, qRT-PCR, WB (Western Blot), Y1H (Yeast One-Hybrid), ChIP-qPCR, EMSA (Electrophoretic Mobility Shift Assay), and D-LUC (D-Luciferin Experiment), and also had a synergistic effect in activating <ce:italic>SlATG13b</ce:italic>. The growth and physiological indexes of VIGS-<ce:italic>SlATG13b</ce:italic> line were significantly lower than those of other groups, and pro-<ce:italic>SlATG13b</ce:italic> is significantly activated by MeJA and saline-alkali signals, so the <ce:italic>SlATG13b</ce:italic> was regulating the resistance of tomato to saline-alkali stress. In summary, the increase of endogenous JA and MeJA content in tomato after saline-alkali stress inhibited the expression and synthesis of <ce:italic>SlJAZ1</ce:italic>, activated the transcription and protein synthesis of <ce:italic>SlWRKY80 and SlWRKY81</ce:italic>, and SlWRKY80 and SlWRKY81 coordinately regulated the transcription of <ce:italic>SlATG13b</ce:italic>, which resulted in the production of more autophagosomes and enhanced the resistance of tomato to saline-alkali stress. This study further improved the molecular mechanism of <ce:italic>SlWRKY80 and SlWRKY81</ce:italic> mediating the JA pathway against saline-alkali stress, and laid a solid theoretical foundation for tomato saline-alkali resistance breeding.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"696 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145956791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-09DOI: 10.1016/j.hpj.2025.08.018
Qisong Li, Yangxin Li, Wenhui Li, Le Sun, Yuanping Li, Linkun Wu, Christopher Rensing, Jianming Zhang, Pumo Cai, Shuaibo Shao
Intercropping tea plants with Ophiopogon japonicus effectively prevents soil erosion and enhances the ecological stability of mountainous tea plantations. However, the underlying ecological facilitation mechanisms remain underexplored. This study investigated the effects of root exudates on rhizosphere soil microbial community structure and tea plant growth under an intercropping system through pot experiments. Results showed that intercropping significantly promoted tea shoot length and plant height. Additionally, rhizosphere microbial diversity increased, with significant changes observed in the microbial community structure. We also analyzed the functional characteristics of the microbiome under different treatments from a microbial functional perspective, such as the enhancement of microbial functions related to glycolysis and cellulose degradation, as well as the enrichment of functional microbes with beneficial genomic traits. Further analysis of root exudate composition under different planting modes revealed that intercropping significantly increased the content of amino acids such as histidine, serine, and leucine in root exudates. KEGG pathway analysis confirmed that metabolic pathways related to amino acid metabolism were significantly enriched, displaying the highest levels of enrichment. These findings highlight the potential of intercropping Ophiopogon japonicus to improve soil ecology and enhance tea plant growth in mountainous plantations.
{"title":"Intercropping Tea Plants with Ophiopogon japonicus Alters Root Exudate Metabolites and Restructures Rhizosphere Microbiota to Promote Plant Growth","authors":"Qisong Li, Yangxin Li, Wenhui Li, Le Sun, Yuanping Li, Linkun Wu, Christopher Rensing, Jianming Zhang, Pumo Cai, Shuaibo Shao","doi":"10.1016/j.hpj.2025.08.018","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.08.018","url":null,"abstract":"Intercropping tea plants with <ce:italic>Ophiopogon japonicus</ce:italic> effectively prevents soil erosion and enhances the ecological stability of mountainous tea plantations. However, the underlying ecological facilitation mechanisms remain underexplored. This study investigated the effects of root exudates on rhizosphere soil microbial community structure and tea plant growth under an intercropping system through pot experiments. Results showed that intercropping significantly promoted tea shoot length and plant height. Additionally, rhizosphere microbial diversity increased, with significant changes observed in the microbial community structure. We also analyzed the functional characteristics of the microbiome under different treatments from a microbial functional perspective, such as the enhancement of microbial functions related to glycolysis and cellulose degradation, as well as the enrichment of functional microbes with beneficial genomic traits. Further analysis of root exudate composition under different planting modes revealed that intercropping significantly increased the content of amino acids such as histidine, serine, and leucine in root exudates. KEGG pathway analysis confirmed that metabolic pathways related to amino acid metabolism were significantly enriched, displaying the highest levels of enrichment. These findings highlight the potential of intercropping <ce:italic>Ophiopogon japonicus</ce:italic> to improve soil ecology and enhance tea plant growth in mountainous plantations.","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"35 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145956877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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