Pub Date : 2025-12-13DOI: 10.1016/j.hpj.2025.09.007
Tao Chen, Jie Yang, Ying Li, Haibo Liu, Minmin Liang, Kang Yong, Minghui Lu
As one of the conserved degradation and clearance systems in eukaryotes, autophagy plays a pivotal role in plant response to environmental stresses, while autophagy–related protein 9 (ATG9), a core protein in the autophagy pathway, serves as a key component for the initiation of ER–derived autophagosomes. In this article, we report that pepper (Capsicum annuum L.) CaATG9 contains two conserved transmembrane domains and one APG9 domain, and has a closer phylogenetic relationship with nightshade SdATG9. CaATG9 is located in the cytoplasm in a dot–like pattern and can respond to diverse abiotic stresses, including heat, salt, drought, as well as abscisic acid (ABA). The silencing of CaATG9 enhances the heat tolerance of pepper, as shown by the lower conductivity, less MDA, H2O2 and dead cells, and the increased expression of heat tolerance–related genes, CaHsfA3, CaHsfA2 and CaHSP25.9, compared to those of the negative control. Correspondingly, the heterologous overexpression of CaATG9 compromises the heat tolerance of tomato. More importantly, the silencing of CaATG9 causes increased stomatal aperture, which promotes the cooling of pepper leaves under heat stress, whereas it exacerbates their water loss under drought stress. These findings suggest that CaATG9 acts as a negative regulator of thermotolerance but a positive regulator of drought and salt tolerance, as evidenced by specific morphological, physiological and molecular indices. Collectively, our study highlights that CaATG9 modulates pepper tolerance to distinct abiotic stresses by influencing stomatal aperture and stress–specific signaling pathways.
自噬是真核生物中保守的降解和清除系统之一，在植物对环境胁迫的反应中起着关键作用，而自噬相关蛋白9 （autophagy - related protein 9, ATG9）是自噬途径的核心蛋白，是er源性自噬体启动的关键成分。在本文中，我们报道了辣椒（Capsicum annuum L.）CaATG9包含两个保守的跨膜结构域和一个APG9结构域，与茄属植物SdATG9有着更密切的系统发育关系。CaATG9位于细胞质中，呈点状分布，可响应多种非生物胁迫，包括热、盐、干旱以及ABA。与阴性对照相比，CaATG9的沉默增强了辣椒的耐热性，表现为电导率降低，MDA、H2O2和死细胞减少，耐热相关基因CaHsfA3、CaHsfA2和CaHSP25.9的表达增加。相应地，CaATG9的异源过表达损害了番茄的耐热性。更重要的是，CaATG9基因的沉默导致气孔开度增大，促进了辣椒叶片在热胁迫下的降温，同时加剧了干旱胁迫下的水分流失。这些结果表明，CaATG9在耐高温方面具有负调控作用，而在耐干旱和耐盐方面具有正调控作用。总的来说，我们的研究强调CaATG9通过影响气孔孔径和胁迫特异性信号通路来调节辣椒对不同非生物胁迫的耐受性。
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Pub Date : 2025-12-12DOI: 10.1016/j.hpj.2025.07.024
Aidi Zhang, Fang Liu, Yuepeng Han, Xiujun Zhang
{"title":"Chromosome-level genome assembly of a blood-fleshed peach ‘ZhongHuaXueTao’ provides new insights into fruit flesh color and maturation","authors":"Aidi Zhang, Fang Liu, Yuepeng Han, Xiujun Zhang","doi":"10.1016/j.hpj.2025.07.024","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.07.024","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"148 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145731104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-12DOI: 10.1016/j.hpj.2025.06.025
Mingxu Niu, Siyu Liu, Mei Li, Abdul Basit, Kaijie Qi, Shaoling Zhang, Katsuhiro Shiratake, Shutian Tao
{"title":"Advances in understanding the regulatory network of pear stone cell formation","authors":"Mingxu Niu, Siyu Liu, Mei Li, Abdul Basit, Kaijie Qi, Shaoling Zhang, Katsuhiro Shiratake, Shutian Tao","doi":"10.1016/j.hpj.2025.06.025","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.06.025","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"230 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145732402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Uncovering the interaction between PatJAZs and PatSWC4 to control the expression of the PatPTS gene and the biosynthesis of patchouli alcohol","authors":"Linyuan Chen, Kun Guo, Ziqian Chen, Yunzi Bao, Liping Wang, Lili Xie, Ruoting Zhan, Likai Chen","doi":"10.1016/j.hpj.2025.06.022","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.06.022","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"71 3 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145689381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-06DOI: 10.1016/j.hpj.2025.05.021
Muhammad Younas, Zheng Zheng, Hongyan Chen, You Li
{"title":"A century-old fight against Huanglongbing with antimicrobial peptides opening the door to citrus health","authors":"Muhammad Younas, Zheng Zheng, Hongyan Chen, You Li","doi":"10.1016/j.hpj.2025.05.021","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.05.021","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"134 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145689384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-12-05DOI: 10.1016/j.hpj.2025.08.016
ZHAO Yongjuan, JIN Yibo, Yao Yutong, Xue Yunjing, Zhang Zhijun, M.A. Zhihang, JIN Lu, Mao Ke, L.I. Chao, M.A. Fengwang
{"title":"Induction of Polyploidy and Physiological Evaluation of Salt Tolerance in Apple","authors":"ZHAO Yongjuan, JIN Yibo, Yao Yutong, Xue Yunjing, Zhang Zhijun, M.A. Zhihang, JIN Lu, Mao Ke, L.I. Chao, M.A. Fengwang","doi":"10.1016/j.hpj.2025.08.016","DOIUrl":"https://doi.org/10.1016/j.hpj.2025.08.016","url":null,"abstract":"","PeriodicalId":13178,"journal":{"name":"Horticultural Plant Journal","volume":"1 1","pages":""},"PeriodicalIF":5.7,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145689386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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