Tej Pratap Jitendra Kumar, A. Ahlawat, Sumit Kumar Singh, N. D. Rathan, A. Rai, Kriti Bajpai, N. Bedi, A. Singh
A set of 16 Near Isogenic Lines (NILs) developed from a cross, Barham x DBW14 for grain softness gene (Pina-D1aPinbD1a) combined with Lr37-Yr17-Sr38 segment was evaluated for different quality traits. Rheological test of flour and baking test with sugar-snap cookies were also performed. The Hardness Index of the NILs ranged between 13.86 and 47.13. The flour of the NILs had relatively lower proportion of particles >150 μm than in the classes of 0 to ≤ 55 μm and > 55 to ≤ 105 μm. Starch damage of all the NILs was significantly lower than the hard grain parent DBW14 (4.54%) and the popular variety HD2967 (7.49%). All the 16 NILs showed optimum Solvent Retention Capacity of NaSRC ≤ 64% and SucroseSRC ≤ 89%. The farinograms of all the NILs were typical of weak gluten with low water absorption capacity. The alveographs showed an Extensibility ratio (P/L) between 0.17 and 0.36, and much lower Deformation Energy (46-94 J) for the NILs. The spread factor of the cookies made with the flour of the NILs varied between 7.90 and 12.77, while that ofthe soft grain parent was 9.44. The findings suggest that the NILs developed in the Indian wheat variety DBW14 may be suitable for production of flour for making cakes, cookies, muffins, etc. These NILs, after evaluation in varietal trials and release for cultivation, can have a strong domestic and export potential. Thus, marker assisted selection is a useful tool for the targeted quality breeding.
以籽粒柔软度基因（Pina-D1aPinbD1a）与Lr37-Yr17-Sr38片段组合的Barham x DBW14为材料，建立了一套16个近等基因系（NIL），对不同品质性状进行了评价。还进行了面粉的流变学试验和糖脆饼干的烘焙试验。NIL的硬度指数在13.86和47.13之间。NIL的面粉中>150μm的颗粒比例相对低于0至≤55μm和>55至≤105μm的面粉。所有NIL的淀粉损伤均显著低于硬粒亲本DBW14（4.54%）和流行品种HD2967（7.49%）。所有16个NIL的最佳溶剂保留能力均为NaSRC≤64%和SucroseSRC≤89%。所有NIL的粉质图都是典型的弱筋低吸水性。瓣膜图显示拉伸比（P/L）在0.17和0.36之间，NIL的变形能（46-94J）低得多。用NIL的面粉制成的饼干的扩散系数在7.90和12.77之间变化，而软粒亲本的扩散系数为9.44。研究结果表明，在印度小麦品种DBW14中开发的NIL可能适合生产蛋糕、饼干、松饼等面粉。这些NIL经过品种试验评估并发布用于种植，具有强大的国内和出口潜力。因此，标记辅助选择是有针对性的优质育种的有用工具。
{"title":"Quality evaluation of near isogenic lines of wheat developed through marker assisted backcross breeding for grain softness","authors":"Tej Pratap Jitendra Kumar, A. Ahlawat, Sumit Kumar Singh, N. D. Rathan, A. Rai, Kriti Bajpai, N. Bedi, A. Singh","doi":"10.31742/ijgpb.82.1.8","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.8","url":null,"abstract":"A set of 16 Near Isogenic Lines (NILs) developed from a cross, Barham x DBW14 for grain softness gene (Pina-D1aPinbD1a) combined with Lr37-Yr17-Sr38 segment was evaluated for different quality traits. Rheological test of flour and baking test with sugar-snap cookies were also performed. The Hardness Index of the NILs ranged between 13.86 and 47.13. The flour of the NILs had relatively lower proportion of particles >150 μm than in the classes of 0 to ≤ 55 μm and > 55 to ≤ 105 μm. Starch damage of all the NILs was significantly lower than the hard grain parent DBW14 (4.54%) and the popular variety HD2967 (7.49%). All the 16 NILs showed optimum Solvent Retention Capacity of NaSRC ≤ 64% and SucroseSRC ≤ 89%. The farinograms of all the NILs were typical of weak gluten with low water absorption capacity. The alveographs showed an Extensibility ratio (P/L) between 0.17 and 0.36, and much lower Deformation Energy (46-94 J) for the NILs. The spread factor of the cookies made with the flour of the NILs varied between 7.90 and 12.77, while that ofthe soft grain parent was 9.44. The findings suggest that the NILs developed in the Indian wheat variety DBW14 may be suitable for production of flour for making cakes, cookies, muffins, etc. These NILs, after evaluation in varietal trials and release for cultivation, can have a strong domestic and export potential. Thus, marker assisted selection is a useful tool for the targeted quality breeding.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45805923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gajala . Ameen, J. Tiwari, V. Prakash, Vivek Kumar Sandilya, B. Das
The major drawback of a dioecious spine gourd (Momordica dioica Roxb.) is distinguishing whether the plant is male or female at an earlier development stage. An investigation was aimed to eliminate aforesaid limitation by identifying and validating sex linked molecular marker. Twenty six male and female genotypes of each sex were screened with 12 specific SSR primers. Only two SSR primers, MdSSR 5 (MADS-box gene of spine gourd) and CMbSSR 6 (MADS-box gene of cucumber) showed amplification and polymorphism in female genotypes only. These specific female linked markers manifest existent of non-epigenetic factors entailed in gender development in the spine gourd.
{"title":"Identification of sex linked molecular markers in spine gourd (Momordica dioica Roxb.)","authors":"Gajala . Ameen, J. Tiwari, V. Prakash, Vivek Kumar Sandilya, B. Das","doi":"10.31742/ijgpb.82.1.17","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.17","url":null,"abstract":"The major drawback of a dioecious spine gourd (Momordica dioica Roxb.) is distinguishing whether the plant is male or female at an earlier development stage. An investigation was aimed to eliminate aforesaid limitation by identifying and validating sex linked molecular marker. Twenty six male and female genotypes of each sex were screened with 12 specific SSR primers. Only two SSR primers, MdSSR 5 (MADS-box gene of spine gourd) and CMbSSR 6 (MADS-box gene of cucumber) showed amplification and polymorphism in female genotypes only. These specific female linked markers manifest existent of non-epigenetic factors entailed in gender development in the spine gourd.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45762745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A study on genetic diversity and principal component analysis (PCA) in a set of 90 fertility restorer lines of rice was conducted to identify potential parents for producing high-yielding hybrids. The D2 statistics uncovered the maximum inter-cluster distance between the clusters having different genotypes. The genotypes PS 2, PRR 828, JR-4322-2, and JR-4322-2 belonging to these diversified gene pools may produce heterotic hybrid combinations involving the most suitable parents mentioned above. The PCA reduces dimensional complexity into nine principle axes with PC1 account maximum variable genotypes, namely, JR-1009, ANP-553, JR-1062-1, JR-1103-1, JR-1023-1, JR-1054-4, IR09N 261, JR-1008, NPT-3806, and JR-81. Considering the magnitude of genetic distance, the per cent contribution of traits towards divergence (fertile spikelet/plant and total spikelet/plant) along with the highest cluster means of these traits reflected by the genotypes belonging to clusters, IX and X. The most quality attributes were included in PC4 and PC5 containing genotypes NPT 10, R710, and JR1301. The diversified gene pool and intensive selection designed for varietal development followed by molecular characterization give a precise idea about the upcoming utilization of existing genetic diversity.
{"title":"Principal component analysis and genetic divergence studies for yield and quality-related attributes of rice restorer lines","authors":"S. Tiwari, Y. Singh, P. K. Upadhyay, G. K. Koutu","doi":"10.31742/ijgpb.82.1.13","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.13","url":null,"abstract":"A study on genetic diversity and principal component analysis (PCA) in a set of 90 fertility restorer lines of rice was conducted to identify potential parents for producing high-yielding hybrids. The D2 statistics uncovered the maximum inter-cluster distance between the clusters having different genotypes. The genotypes PS 2, PRR 828, JR-4322-2, and JR-4322-2 belonging to these diversified gene pools may produce heterotic hybrid combinations involving the most suitable parents mentioned above. The PCA reduces dimensional complexity into nine principle axes with PC1 account maximum variable genotypes, namely, JR-1009, ANP-553, JR-1062-1, JR-1103-1, JR-1023-1, JR-1054-4, IR09N 261, JR-1008, NPT-3806, and JR-81. Considering the magnitude of genetic distance, the per cent contribution of traits towards divergence (fertile spikelet/plant and total spikelet/plant) along with the highest cluster means of these traits reflected by the genotypes belonging to clusters, IX and X. The most quality attributes were included in PC4 and PC5 containing genotypes NPT 10, R710, and JR1301. The diversified gene pool and intensive selection designed for varietal development followed by molecular characterization give a precise idea about the upcoming utilization of existing genetic diversity.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45780577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In the present investigation, Strychnos nux vomica L. and Strychnos wallichiana Steud. ex A. DC. have been selected for karyotypic investigation to strengthen genetic relationships. Chromosome number recorded in S.nux vomica is 2n=32 while in S. wallichianais 2n=40. In both species A-type, B-type and C-type chromosomes were observed with 28 metacentric chromosomes and 4 sub-metacentric in S. nux vomica and 38 metacentric and 2 sub-metacentric chromosomes in S. wallichiana. The total form per cent (T.F %), Dispersion Index (DI), Asymmetry index (AI) depict the symmetric karyotype of chromosomes which represents their primitive level in the family.
{"title":"Karyomorphological studies in two species of genus Strychnos L. significant in ethno-pharmacological aspects","authors":"Aswathy Ravindran, B. Das","doi":"10.31742/ijgpb.82.1.19","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.19","url":null,"abstract":"In the present investigation, Strychnos nux vomica L. and Strychnos wallichiana Steud. ex A. DC. have been selected for karyotypic investigation to strengthen genetic relationships. Chromosome number recorded in S.nux vomica is 2n=32 while in S. wallichianais 2n=40. In both species A-type, B-type and C-type chromosomes were observed with 28 metacentric chromosomes and 4 sub-metacentric in S. nux vomica and 38 metacentric and 2 sub-metacentric chromosomes in S. wallichiana. The total form per cent (T.F %), Dispersion Index (DI), Asymmetry index (AI) depict the symmetric karyotype of chromosomes which represents their primitive level in the family.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44526555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Mehta, D. Basandrai, V. Rana, H. Dhillon, A. Basandrai
The analysis of data on disease severity using genotype (G) and genotype by environment interaction (GGE) biplot revealed that the highest contribution to disease severity was due to genotype (G) 50.75 and 47.56 followed by G X E interaction 44.38; 37.70 and environment (E) 4.8 and 14.74 for powdery mildew (PM) and yellow rust (YR), respectively. Sixteen genotypes showing mean TDS ≤15% and Area under Disease Progress Curve (AUDPC), relative Area Under Disease Progress Curve (rAUDPC) and ‘r’ ranging between 20.63-494.44, 2.75-40.05 and 0.01-0.04, respectively in comparison with TDS, AUDPC, rAUDPC and ‘r’ of 41%, 1510.88, 99.18 and 0.04 respectively, in susceptible cultivar Lehmi, were slow mildewing genotypes. Six genotypes Maris dove, Rye, Aldan, CMH 77.308, SAW 71 and SAW 74 showed multiple resistances to PM and YR, of which ALDAN and CMH 77.308 were reported to be resistant to Karnal bunt (KB) also. Hence, these can be used as potential donors aimed to develop cultivars with combined resistance to PM, YR and /or KB and Kukumseri could be used as an ideal hot spot for screening against PM and YR.
{"title":"Deciphering field resistance to powdery mildew and yellow rust among popular cultivars of wheat and set of differential lines","authors":"A. Mehta, D. Basandrai, V. Rana, H. Dhillon, A. Basandrai","doi":"10.31742/ijgpb.82.1.6","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.6","url":null,"abstract":"The analysis of data on disease severity using genotype (G) and genotype by environment interaction (GGE) biplot revealed that the highest contribution to disease severity was due to genotype (G) 50.75 and 47.56 followed by G X E interaction 44.38; 37.70 and environment (E) 4.8 and 14.74 for powdery mildew (PM) and yellow rust (YR), respectively. Sixteen genotypes showing mean TDS ≤15% and Area under Disease Progress Curve (AUDPC), relative Area Under Disease Progress Curve (rAUDPC) and ‘r’ ranging between 20.63-494.44, 2.75-40.05 and 0.01-0.04, respectively in comparison with TDS, AUDPC, rAUDPC and ‘r’ of 41%, 1510.88, 99.18 and 0.04 respectively, in susceptible cultivar Lehmi, were slow mildewing genotypes. Six genotypes Maris dove, Rye, Aldan, CMH 77.308, SAW 71 and SAW 74 showed multiple resistances to PM and YR, of which ALDAN and CMH 77.308 were reported to be resistant to Karnal bunt (KB) also. Hence, these can be used as potential donors aimed to develop cultivars with combined resistance to PM, YR and /or KB and Kukumseri could be used as an ideal hot spot for screening against PM and YR.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48303567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Mohan, H. M. Mamrutha, Rinki Khobra, Gyanendra Singh, G. P. Singh
Yield improvement in wheat through grain number and grain weight has become increasingly difficult in many production environments. Can the non-grain yield parameters of yield like height, flowering and maturity be explored as an alternate for further yield enhancement? This study comprehends such possibilities by analysing performance of the released and pre-released varieties evaluated in ten diverse production environments of India during the period 2000-2021. Regression analysis has highlighted relevance of these three crop expressions in wheat yield and based upon their contribution, selection criteria has been suggested for different production environments of India. Magnitude of impact varied under each environment and their collective contribution was highest in a production environment where growth condition was most favourable for wheat growth i.e., north-western plains. Increase in height and maturity duration coupled with early heading was found useful in this region for both wheat category, i.e., timely-sown and late-sown. These three yield determinants were found highly effective in timely-sown of the adjoining north-eastern plains also but under late-sown condition, prolonged heading was the chief yield predictor. Height for timely-sown and maturity for late-sown condition was most crucial for central India. Delayed heading in timely-sown and late flowering with extra height in late-sown wheat had been tipped for yield improvement in peninsular India. Delayed maturity for timely-sown and height for late-sown wheat had been suggested for productivity enhancement in the northern hills. Besides grain yield, this selection strategy based upon height and crop phenology also enhanced grain weight in certain environments. At a time when selection for grain attributes is not providing further push in productivity enhancement; it would be worth trying to explore these non-grain field indicators as selection strategy for further advancement in productivity of bread wheat.
{"title":"Relevance of height, heading and maturity in productivity enhancement of wheat","authors":"D. Mohan, H. M. Mamrutha, Rinki Khobra, Gyanendra Singh, G. P. Singh","doi":"10.31742/ijgpb.82.1.5","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.5","url":null,"abstract":"Yield improvement in wheat through grain number and grain weight has become increasingly difficult in many production environments. Can the non-grain yield parameters of yield like height, flowering and maturity be explored as an alternate for further yield enhancement? This study comprehends such possibilities by analysing performance of the released and pre-released varieties evaluated in ten diverse production environments of India during the period 2000-2021. Regression analysis has highlighted relevance of these three crop expressions in wheat yield and based upon their contribution, selection criteria has been suggested for different production environments of India. Magnitude of impact varied under each environment and their collective contribution was highest in a production environment where growth condition was most favourable for wheat growth i.e., north-western plains. Increase in height and maturity duration coupled with early heading was found useful in this region for both wheat category, i.e., timely-sown and late-sown. These three yield determinants were found highly effective in timely-sown of the adjoining north-eastern plains also but under late-sown condition, prolonged heading was the chief yield predictor. Height for timely-sown and maturity for late-sown condition was most crucial for central India. Delayed heading in timely-sown and late flowering with extra height in late-sown wheat had been tipped for yield improvement in peninsular India. Delayed maturity for timely-sown and height for late-sown wheat had been suggested for productivity enhancement in the northern hills. Besides grain yield, this selection strategy based upon height and crop phenology also enhanced grain weight in certain environments. At a time when selection for grain attributes is not providing further push in productivity enhancement; it would be worth trying to explore these non-grain field indicators as selection strategy for further advancement in productivity of bread wheat.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42676083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. ., K. Noerwijati, S. Indiati, M. J. Mejaya, H. Kuswantoro
The experiments were done during 2015 to 2018 in eight environments considering climate and soil chemistry factors. The evaluation of genotypes was carried out in early root bulking cassava to identify the promising ones for tuber yield stability based on Additive Effects and Multiplicative Interaction (AMMI () biplot analysis. Results showed that genotypes, CMR 51-61-1, CMR 51-48-17, CMR 51-48-16, OMR 51-20-5, OMM0806 - 57, UJ3 and UJ5 were most stable as compared to CMR 51-07-13 and CMR 51-06-16. The important environmental factors affecting yield stability of cassava promising genotypes based on tuber yield in seven months crop duration were N and P2O5 contents and pH on topsoil. Mean fresh tuber yield of OMR 51-20-5 was 25% significantly higher than UJ3 and the increasing value was equal to US $ 745/ha while of OMR 51-20-5, it was 15% higher than UJ5 and the increasing value was equal to US $ 482/ha. The genotype, OMR 51-20-5 has been released recently in 2020 as VAMAS 1 by the Indonesian Government.
{"title":"Evaluation of cassava (Manihot esculenta Cranzt.) genotypes for resistance to mite and yield stability through AMMI analysis","authors":"S. ., K. Noerwijati, S. Indiati, M. J. Mejaya, H. Kuswantoro","doi":"10.31742/ijgpb.82.1.12","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.12","url":null,"abstract":"The experiments were done during 2015 to 2018 in eight environments considering climate and soil chemistry factors. The evaluation of genotypes was carried out in early root bulking cassava to identify the promising ones for tuber yield stability based on Additive Effects and Multiplicative Interaction (AMMI () biplot analysis. Results showed that genotypes, CMR 51-61-1, CMR 51-48-17, CMR 51-48-16, OMR 51-20-5, OMM0806 - 57, UJ3 and UJ5 were most stable as compared to CMR 51-07-13 and CMR 51-06-16. The important environmental factors affecting yield stability of cassava promising genotypes based on tuber yield in seven months crop duration were N and P2O5 contents and pH on topsoil. Mean fresh tuber yield of OMR 51-20-5 was 25% significantly higher than UJ3 and the increasing value was equal to US $ 745/ha while of OMR 51-20-5, it was 15% higher than UJ5 and the increasing value was equal to US $ 482/ha. The genotype, OMR 51-20-5 has been released recently in 2020 as VAMAS 1 by the Indonesian Government.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46258544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Vineeth, Indivar Prasad, A. Chinchmalatpure, B. Lokeshkumar, Shrvan Kumar, K. T. Ravikiran, P. C. Sharma, Sharma P. C 2022. Weighted
Cotton is a major cash crop classified as moderately tolerant to salt stress (threshold ECe = 7.7 dS m-1). In the present study, a set of 21 homozygous advanced breeding lines of cotton (Gossypium herbaceum L.), including one salt tolerant variety G Cot 23 was evaluated over three years to identify stable high yielding genotypes under salt stress. Weighted Average Absolute Scores of BLUPs (WAASB) stability index, which is based on single value decomposition of BLUP, was employed for this purpose. Among the 21 genotypes, CSC-025 and CSC-057 showed the highest boll weight (59.67 and 57.33 g/20 boll), seed cotton yield (1818 and 1570 Kg ha-1) and leaf K/Na ratio (9.6 and 5.6) over the check variety G Cot 23. Considering both WAASB stability index and mean trait values, CSC-025, CSC-057 and G Cot 23 were found promising for all traits and can be considered for deployment in salt-affected Vertisols of India.
{"title":"Weighted average absolute scores of BLUPs (WAASB) based selection of stable Asiatic cotton genotypes for the salt affected Vertisols of India","authors":"T. Vineeth, Indivar Prasad, A. Chinchmalatpure, B. Lokeshkumar, Shrvan Kumar, K. T. Ravikiran, P. C. Sharma, Sharma P. C 2022. Weighted","doi":"10.31742/ijgpb.82.1.15","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.15","url":null,"abstract":"Cotton is a major cash crop classified as moderately tolerant to salt stress (threshold ECe = 7.7 dS m-1). In the present study, a set of 21 homozygous advanced breeding lines of cotton (Gossypium herbaceum L.), including one salt tolerant variety G Cot 23 was evaluated over three years to identify stable high yielding genotypes under salt stress. Weighted Average Absolute Scores of BLUPs (WAASB) stability index, which is based on single value decomposition of BLUP, was employed for this purpose. Among the 21 genotypes, CSC-025 and CSC-057 showed the highest boll weight (59.67 and 57.33 g/20 boll), seed cotton yield (1818 and 1570 Kg ha-1) and leaf K/Na ratio (9.6 and 5.6) over the check variety G Cot 23. Considering both WAASB stability index and mean trait values, CSC-025, CSC-057 and G Cot 23 were found promising for all traits and can be considered for deployment in salt-affected Vertisols of India.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42960071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Sunusi, Asadullah Khan, Y. Tsago, Ziyan Chen, C. Shi, Xiaoli Jin
Grain size is one of the important agronomic traits that determine rice yield. Map-based cloning of rice grain size-related genes impacts the genetic regulation mechanism of rice yield formation. Here we identified bgl mutant in rice which exhibits increase in grain length and panicle length with the decrease in number of grains per panicle and no observable difference in grain thickness and number of tillers as compared to the wild type (WT). Scanning electron microscopy observation of bgl mutant spikelets hull indicates an upsurge in number of external epidermal cells in a longitudinal way than the WT. Hence, it is likely that the long-grain phenotype was due to increased cell number longitudinally. Starch granule in both bgl and WT were large, sporadically polyhedral and densely packed, indicating no differences, signifying that both bgl mutant and WT have less chalkiness content. Genetic analysis showed that the bgl mutant was controlled by a recessive single gene, and was finely located on chromosome 3 through map-based cloning, within a physical distance of 40kb. Sequencing analysis revealed A to T substitution in the seventeen exons of LOC_Os03g44500 resulted in an amino acid change from Tryptophan (Try) to Phenylalanine (Phe). Taken together bgl mutant has a pleiotropic effect on grain yield and grain quality.
{"title":"Genetic analysis and gene mapping of bgl, a gene controlling grain length and quality in rice","authors":"M. Sunusi, Asadullah Khan, Y. Tsago, Ziyan Chen, C. Shi, Xiaoli Jin","doi":"10.31742/ijgpb.82.1.3","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.3","url":null,"abstract":"Grain size is one of the important agronomic traits that determine rice yield. Map-based cloning of rice grain size-related genes impacts the genetic regulation mechanism of rice yield formation. Here we identified bgl mutant in rice which exhibits increase in grain length and panicle length with the decrease in number of grains per panicle and no observable difference in grain thickness and number of tillers as compared to the wild type (WT). Scanning electron microscopy observation of bgl mutant spikelets hull indicates an upsurge in number of external epidermal cells in a longitudinal way than the WT. Hence, it is likely that the long-grain phenotype was due to increased cell number longitudinally. Starch granule in both bgl and WT were large, sporadically polyhedral and densely packed, indicating no differences, signifying that both bgl mutant and WT have less chalkiness content. Genetic analysis showed that the bgl mutant was controlled by a recessive single gene, and was finely located on chromosome 3 through map-based cloning, within a physical distance of 40kb. Sequencing analysis revealed A to T substitution in the seventeen exons of LOC_Os03g44500 resulted in an amino acid change from Tryptophan (Try) to Phenylalanine (Phe). Taken together bgl mutant has a pleiotropic effect on grain yield and grain quality.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47671881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Sharma, S. Thakur, H. Sankhyan, S. Jha, R. Sharma, Poonam Kanwar, V. Gautam, N. Sankhyan
Indian willow (Salix tetrasperma) is an agriculturally useful tree which occurs over a wide geographic area across South Asia and bears importance. So far, this species has never been studied for molecular genetic diversity. The present study was, therefore, carried out to assess the genetic diversity and population structure analysis using RAPD and ISSR molecular markers in diverse genotypes from five populations covering North India. The mean number of effective alleles, Shannon information index and gene diversity i.e., 1.38 ± 0.013, 0.35 ± 0.010 and 0.23 ± 0.007, respectively were obtained with RAPD + ISSR markers. The analysis of molecular variance generated by RAPD + ISSR revealed a higher genetic variation (87%) within population as compared to that of among population (13%). Nei genetic distance was maximum (0.185) between Jammu and Kashmir and Punjab populations. Significant Mantel correlation (r=0.551,) was obtained between RAPD and ISSR markers. Bayesian clustering pattern obtained through STRUCTURE software showed four gene pools. Based on the genetic information obtained with regards to Indian willow by combining the RAPD and ISSR marker systems, it is proposed that an individual tree be selected within populations rather than among populations for the improvement of economic traits of Indian willow alongwith conservation of entire ecological populations.
{"title":"Genetic diversity and population structure of Indian willow (Salix tetrasperma Roxb.) with dominant molecular markers along its distribution range in Himalayan region","authors":"J. Sharma, S. Thakur, H. Sankhyan, S. Jha, R. Sharma, Poonam Kanwar, V. Gautam, N. Sankhyan","doi":"10.31742/ijgpb.82.1.10","DOIUrl":"https://doi.org/10.31742/ijgpb.82.1.10","url":null,"abstract":"Indian willow (Salix tetrasperma) is an agriculturally useful tree which occurs over a wide geographic area across South Asia and bears importance. So far, this species has never been studied for molecular genetic diversity. The present study was, therefore, carried out to assess the genetic diversity and population structure analysis using RAPD and ISSR molecular markers in diverse genotypes from five populations covering North India. The mean number of effective alleles, Shannon information index and gene diversity i.e., 1.38 ± 0.013, 0.35 ± 0.010 and 0.23 ± 0.007, respectively were obtained with RAPD + ISSR markers. The analysis of molecular variance generated by RAPD + ISSR revealed a higher genetic variation (87%) within population as compared to that of among population (13%). Nei genetic distance was maximum (0.185) between Jammu and Kashmir and Punjab populations. Significant Mantel correlation (r=0.551,) was obtained between RAPD and ISSR markers. Bayesian clustering pattern obtained through STRUCTURE software showed four gene pools. Based on the genetic information obtained with regards to Indian willow by combining the RAPD and ISSR marker systems, it is proposed that an individual tree be selected within populations rather than among populations for the improvement of economic traits of Indian willow alongwith conservation of entire ecological populations.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":1.0,"publicationDate":"2022-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44188700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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