A. Das, A. Singode, D. Chaudhary, Yathish Kr, Chikkappa Gangadhar Karjagi, Ramesh Kumar, B. Kumar, Vishal Singh, G. Mukri, Sapna, S. Rakshit
Vitamin A deficiency (VAD) is a global health problem. Maize naturally accumulates carotenoids including ?-carotene, a major source of vitamin A. Thirty-seven Indian maize genotypes were screened for total carotenoids and ?carotene content. A positive significant association of kernel colour was observed with total carotenoid but not for ?-carotene. The level of ?-carotene among the selected 37 genotypes was low (0.14 to 4.63µg/g) as compared to total carotenoids (15.94 to 66.46 µg/g) thus necessitates the introduction and deployment of lines with high ?carotene. Hence, 54 inbred lines developed under the HarvestPlus programme were screened with functional markers of lcyE and crtRB1 genes of the carotenoid biosynthetic pathway. Three genotypes (HP704-13, HP704- 22, and HP704-23) amplified most of the favorable alleles for both these genes. However, due to long Anthesis Silking Interval (ASI) and poor adaptation HP704-13 and HP704-23 could not be maintained. Under normal storage condition genotype, HP704-22 recorded 7.32 µg/g of ?-carotene which can be used as a donor for provitamin-A or to diversify the Indian germplasm.
{"title":"Identification of potential donor for pro-vitamin A using functional markers in maize (Zea mays L)","authors":"A. Das, A. Singode, D. Chaudhary, Yathish Kr, Chikkappa Gangadhar Karjagi, Ramesh Kumar, B. Kumar, Vishal Singh, G. Mukri, Sapna, S. Rakshit","doi":"10.31742/ijgpb.81.1.5","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.5","url":null,"abstract":"Vitamin A deficiency (VAD) is a global health problem. Maize naturally accumulates carotenoids including ?-carotene, a major source of vitamin A. Thirty-seven Indian maize genotypes were screened for total carotenoids and ?carotene content. A positive significant association of kernel colour was observed with total carotenoid but not for ?-carotene. The level of ?-carotene among the selected 37 genotypes was low (0.14 to 4.63µg/g) as compared to total carotenoids (15.94 to 66.46 µg/g) thus necessitates the introduction and deployment of lines with high ?carotene. Hence, 54 inbred lines developed under the HarvestPlus programme were screened with functional markers of lcyE and crtRB1 genes of the carotenoid biosynthetic pathway. Three genotypes (HP704-13, HP704- 22, and HP704-23) amplified most of the favorable alleles for both these genes. However, due to long Anthesis Silking Interval (ASI) and poor adaptation HP704-13 and HP704-23 could not be maintained. Under normal storage condition genotype, HP704-22 recorded 7.32 µg/g of ?-carotene which can be used as a donor for provitamin-A or to diversify the Indian germplasm.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45120316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Islam, S. Shivaraj, Prof Vikas Kumar, D. S. Phad, H. Sonah, S. B. Tripathi, R. Deshmukh
In the present study, a total of 27 chloroplast specific SSRs (CpSSR) have been identified in the chloroplast genome of Capsicum annum L. The frequency of the SSRs was about one in 5.7 kb of the chloroplast genome. Out of 27 SSRs, 26 were mono-nucleotide repeats of A/T and one was a trinucleotide repeat (TTA). Further a set of seven markers were validated by genotyping 48 capsicum accessions comprising of cultivars from five different species and landraces of unknown identity. The seven SSR markers generated a total of 27 alleles among 48 samples used in this study. The size of the amplicons varied from 161 bp (CaCpM22 and 26) to 339 bp (CaCpM06). The polymorphic information content (PIC) value for the set of the primers used ranged from 0.11 to 0.48 with an average of 0.33. The number of alleles for markers ranged from three to six with an average of 3.28 alleles per marker. The phylogenetic analysis of the chilly accessions showed that the Bhut jolokia land race is clustered along with the C. frutescence indicating the it’s probable parentage. The chloroplast genome based SSR markers identified in the present study can be further used for the marker-assisted genomic studies
本研究共在辣椒叶绿体基因组中鉴定出27个叶绿体特异性ssr (CpSSR)，频率约为1 / 5.7 kb。在27个SSRs中，26个是A/T的单核苷酸重复序列，1个是三核苷酸重复序列(TTA)。通过对来自5个不同品种和未知地方品种的48份辣椒材料进行基因分型，验证了7个标记的有效性。在本研究使用的48份样品中，7个SSR标记共产生27个等位基因。扩增子的大小从161 bp (CaCpM22和26)到339 bp (CaCpM06)不等。引物的多态性信息含量(PIC)值在0.11 ~ 0.48之间，平均为0.33。每个标记的等位基因数为3 ~ 6个，平均为3.28个。系统发育分析表明，山核桃陆生人种与山核桃陆生人种聚集在一起，表明山核桃陆生人种可能是其亲本。本研究鉴定的基于叶绿体基因组的SSR标记可进一步用于标记辅助基因组研究
{"title":"Development of chloroplast microsatellite markers in Capsicum: Insight into evolution of Bhut Jolokia - a clad of ghost chilli landraces","authors":"A. Islam, S. Shivaraj, Prof Vikas Kumar, D. S. Phad, H. Sonah, S. B. Tripathi, R. Deshmukh","doi":"10.31742/ijgpb.81.1.10","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.10","url":null,"abstract":"In the present study, a total of 27 chloroplast specific SSRs (CpSSR) have been identified in the chloroplast genome of Capsicum annum L. The frequency of the SSRs was about one in 5.7 kb of the chloroplast genome. Out of 27 SSRs, 26 were mono-nucleotide repeats of A/T and one was a trinucleotide repeat (TTA). Further a set of seven markers were validated by genotyping 48 capsicum accessions comprising of cultivars from five different species and landraces of unknown identity. The seven SSR markers generated a total of 27 alleles among 48 samples used in this study. The size of the amplicons varied from 161 bp (CaCpM22 and 26) to 339 bp (CaCpM06). The polymorphic information content (PIC) value for the set of the primers used ranged from 0.11 to 0.48 with an average of 0.33. The number of alleles for markers ranged from three to six with an average of 3.28 alleles per marker. The phylogenetic analysis of the chilly accessions showed that the Bhut jolokia land race is clustered along with the C. frutescence indicating the it’s probable parentage. The chloroplast genome based SSR markers identified in the present study can be further used for the marker-assisted genomic studies","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41971811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Srivastav, S. Singh, J. Prakash, Rakesh Singh, N. Sharma, Sridhar Ramchandra, Rajni Devi, Ankit Gupta, A. K. Mahto, P. Jayaswal, Sangeeta Singh, N. Singh
Whole genome sequence in mango offers unprecedented opportunities for genomics assisted crop improvement via enabling access to genome-wide genetic markers. In the present study, simple sequence repeats (SSRs) were predicted from genome sequence of mango. Based on the SSR length (? 50 bp), highly-variable mango SSRs (MSSRs) were sorted. A sub-set of 129 MSSRs was validated on a set of 24 diverse mango genotypes yielding marker validation and polymorphism of 88.96 and 85.27 per cent, respectively. One hundred and ten polymorphic markers were identified for the present set of mango genotypes. Polymorphic information content (PIC) ranged from 0.10 to 0.78 and the highest value was observed with MSSR133. The mean PIC value was 0.40 but 33 MSSR markers showed PIC values ? 0.5, suggesting that these markers can efficiently measure genetic diversity and serve for mapping of quantitative trait loci (QTLs) in mango. MSSRs data was further used for diversity analysis of mango genotypes belonging to different agro-ecological conditions including chance seedlings, landraces, exotic and indigenous germplasm and hybrids. Cluster analysis using UPGMA of 24 mango genotypes revealed that these MSSRs were informative in diversity analysis and distinguished mango genotypes based on their origin, parentage and embryo types. A novel set of 110 hyper-variable SSR markers have been added to the mango repository depicting usefulness in discriminating closely related mango germplasm and their use in mango improvement programme.
{"title":"New hyper-variable SSRs for diversity analysis in mango (Mangifera indica L.)","authors":"M. Srivastav, S. Singh, J. Prakash, Rakesh Singh, N. Sharma, Sridhar Ramchandra, Rajni Devi, Ankit Gupta, A. K. Mahto, P. Jayaswal, Sangeeta Singh, N. Singh","doi":"10.31742/ijgpb.81.1.13","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.13","url":null,"abstract":"Whole genome sequence in mango offers unprecedented opportunities for genomics assisted crop improvement via enabling access to genome-wide genetic markers. In the present study, simple sequence repeats (SSRs) were predicted from genome sequence of mango. Based on the SSR length (? 50 bp), highly-variable mango SSRs (MSSRs) were sorted. A sub-set of 129 MSSRs was validated on a set of 24 diverse mango genotypes yielding marker validation and polymorphism of 88.96 and 85.27 per cent, respectively. One hundred and ten polymorphic markers were identified for the present set of mango genotypes. Polymorphic information content (PIC) ranged from 0.10 to 0.78 and the highest value was observed with MSSR133. The mean PIC value was 0.40 but 33 MSSR markers showed PIC values ? 0.5, suggesting that these markers can efficiently measure genetic diversity and serve for mapping of quantitative trait loci (QTLs) in mango. MSSRs data was further used for diversity analysis of mango genotypes belonging to different agro-ecological conditions including chance seedlings, landraces, exotic and indigenous germplasm and hybrids. Cluster analysis using UPGMA of 24 mango genotypes revealed that these MSSRs were informative in diversity analysis and distinguished mango genotypes based on their origin, parentage and embryo types. A novel set of 110 hyper-variable SSR markers have been added to the mango repository depicting usefulness in discriminating closely related mango germplasm and their use in mango improvement programme.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42539120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assessment of genetic diversity between selected genotypes serves as an essential tool to exploit the genetic variability. In the present study, Ninety nine accessions of oat acquired from different geographical regions were used to study the molecular genetic variability by the use of 91 microsatellite primers. A total of 248 alleles from 91 primer pairs in the 99 oat genotypes were detected. The average alleles per primer pair were recorded as 2.69. The primer pairs having the highest PIC values viz., AM 6 (0.50), AM 55 (0.49) and M 83381 (0.49) can be further used in the association mapping studies. Clustering of the oat genotypes into two main clusters A and B was done by using the symmetric matrix of DICE coefficient based on UPGMA method in Software – DARwin 6.0. In this study, the moderate amount of dissimilarity was observed between the whole set of accessions, the values ranging from 0.33 to 0.79. The individuals belonging to the different clusters may be useful in hybridization studies of oat improvement programmes.
选择基因型之间的遗传多样性评估是开发遗传变异的重要工具。利用91条微卫星引物，对不同地理区域的99份燕麦材料进行分子遗传变异研究。共检测到99个燕麦基因型中91对引物的248个等位基因。每对引物平均等位基因数为2.69个。PIC值最高的引物对AM 6(0.50)、AM 55(0.49)和M 83381(0.49)可进一步用于关联图谱研究。在软件- DARwin 6.0中基于UPGMA方法，利用DICE系数对称矩阵将燕麦基因型聚类为A和B两个主要聚类。在本研究中，全组材料间的差异值为0.33 ~ 0.79。属于不同簇的个体可能在燕麦改良方案的杂交研究中有用。
{"title":"Molecular characterization of oats (Avena sativa L.) germplasm with microsatellite markers","authors":"G. Kaur, R. Kapoor, Priti Sharma, P. Srivastava","doi":"10.31742/ijgpb.81.1.18","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.18","url":null,"abstract":"Assessment of genetic diversity between selected genotypes serves as an essential tool to exploit the genetic variability. In the present study, Ninety nine accessions of oat acquired from different geographical regions were used to study the molecular genetic variability by the use of 91 microsatellite primers. A total of 248 alleles from 91 primer pairs in the 99 oat genotypes were detected. The average alleles per primer pair were recorded as 2.69. The primer pairs having the highest PIC values viz., AM 6 (0.50), AM 55 (0.49) and M 83381 (0.49) can be further used in the association mapping studies. Clustering of the oat genotypes into two main clusters A and B was done by using the symmetric matrix of DICE coefficient based on UPGMA method in Software – DARwin 6.0. In this study, the moderate amount of dissimilarity was observed between the whole set of accessions, the values ranging from 0.33 to 0.79. The individuals belonging to the different clusters may be useful in hybridization studies of oat improvement programmes.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46693230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thirty crosses developed through line × tester mating design were evaluated along with parents in three environments to estimate gca and sca effects of parents and crosses, respectively for yield and its contributing traits. Based on consistent performance over three environments in safflower (Carthamus tinctorious L.) genotypes, BHIMA, GMU 3876, GMU 3863, GMU 7351, GMU 2757, AKS 08R and AKS CMS 2B were found to be good general combiners for yield and most of yield contributing characters. Hybrids, AKS CMS 2A x BHIMA, AKS CMS 2A x GMU 3876 and AKS CMS 2A x GMU 3863 having high sca effects were found promising for seed yield per plant and other traits over the three environments. The results indicated that these parents could be exploited in hybridization programmes for genetic improvement of individual traits. However, the promising hybrids having high sca effects may be used for heterosis breeding after multi-locations testing.
{"title":"Selection of elite parents and crosses for seed yield and its components using combining ability analysis over the three environments in safflower (Carthamus tinctorious L.)","authors":"Indrayani H. Thorat, V. Gawande","doi":"10.31742/ijgpb.81.1.17","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.17","url":null,"abstract":"Thirty crosses developed through line × tester mating design were evaluated along with parents in three environments to estimate gca and sca effects of parents and crosses, respectively for yield and its contributing traits. Based on consistent performance over three environments in safflower (Carthamus tinctorious L.) genotypes, BHIMA, GMU 3876, GMU 3863, GMU 7351, GMU 2757, AKS 08R and AKS CMS 2B were found to be good general combiners for yield and most of yield contributing characters. Hybrids, AKS CMS 2A x BHIMA, AKS CMS 2A x GMU 3876 and AKS CMS 2A x GMU 3863 having high sca effects were found promising for seed yield per plant and other traits over the three environments. The results indicated that these parents could be exploited in hybridization programmes for genetic improvement of individual traits. However, the promising hybrids having high sca effects may be used for heterosis breeding after multi-locations testing.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42910185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Three hill cotton (Gossypium arboreum L.) varieties viz., HC-1, HC-2 and HC-3, released by Bangladesh Cotton Development Board were investigated through orcein, CMAand DAPI-banding for cytogenetical characterization and to elucidate the karyotypic diversity among these varieties. All these three varieties were found to possess 2n = 26 metacentric chromosomes with ‘1A’ karyotype. Based on TF%, AsK% and Syi index, HC-3 was little advanced over HC-1 and HC-2. These three varieties showed differential Chromomycin A3 (CMA)- and 4Ê-6 Diamidino-2-Phenyl Indole (DAPI)-banding patterns and a tendency of acumulation of repetitive sequences at the terminal regions was observed. Despite possessing same somatic chromosome number these three hill cotton varieties could be characterized by diversified karyotypic parameters through differential staining.
{"title":"Cytogenetic studies of three cultivated hill cotton (Gossypium arboreum L.) varieties from Bangladesh","authors":"A. Hossain, C. Dash, Syeda Sharmeen Sultana","doi":"10.31742/ijgpb.81.1.16","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.16","url":null,"abstract":"Three hill cotton (Gossypium arboreum L.) varieties viz., HC-1, HC-2 and HC-3, released by Bangladesh Cotton Development Board were investigated through orcein, CMAand DAPI-banding for cytogenetical characterization and to elucidate the karyotypic diversity among these varieties. All these three varieties were found to possess 2n = 26 metacentric chromosomes with ‘1A’ karyotype. Based on TF%, AsK% and Syi index, HC-3 was little advanced over HC-1 and HC-2. These three varieties showed differential Chromomycin A3 (CMA)- and 4Ê-6 Diamidino-2-Phenyl Indole (DAPI)-banding patterns and a tendency of acumulation of repetitive sequences at the terminal regions was observed. Despite possessing same somatic chromosome number these three hill cotton varieties could be characterized by diversified karyotypic parameters through differential staining.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49535482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kumar, V. Ramya, C. S. Kumar, T. Raju, N. Kumar, G. Seshu, G. Sathish, D. Bhadru, M. .. Ramana
Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important pulse crop grown under Indian rainfed agriculture. Twenty eight pigeonpea genotypes were tested for stability and adaptability across ten rainfed locations in the States of Telangana and Karnataka, India using AMMI (additive main effects and multiplicative interaction) model and GGE (genotype and genotype by environment) biplot method. The grain yields were significantly affected by environment (56.8%) followed by genotype × environment interaction (27.6%) and genotype (18.6%) variances. Two mega environments were identified with several winning genotypes viz., ICPH 2740 (G15), TS 3R (G10), PRG 176 (G8) and ICPL 96058 (G22). E2 (Gulbarga, Karnataka), E3 (Bidar, Karnataka) and E6 (Vikarabad, Telangana) were the most discriminating environments. Genotypes, ICPH 2740, PRG 176 and TS 3R were the best cultivars in all the environments whereas PRG 158 (G9), ICPL 87119 (G12), ICPL 20098 (G19) and ICPL 96058 (G22) were suitable across a wide range of environments. Genotypes, ICPH 2740 and PRG 176 can be recommended on a large scale to the farmers with small holdings to enhance pigeonpea productivity and improve the food security
{"title":"Identification of pigeonpea genotypes with wider adaptability to rainfed environments through AMMI and GGE biplot analyses","authors":"M. Kumar, V. Ramya, C. S. Kumar, T. Raju, N. Kumar, G. Seshu, G. Sathish, D. Bhadru, M. .. Ramana","doi":"10.31742/ijgpb.81.1.7","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.7","url":null,"abstract":"Pigeonpea [Cajanus cajan (L.) Millspaugh] is an important pulse crop grown under Indian rainfed agriculture. Twenty eight pigeonpea genotypes were tested for stability and adaptability across ten rainfed locations in the States of Telangana and Karnataka, India using AMMI (additive main effects and multiplicative interaction) model and GGE (genotype and genotype by environment) biplot method. The grain yields were significantly affected by environment (56.8%) followed by genotype × environment interaction (27.6%) and genotype (18.6%) variances. Two mega environments were identified with several winning genotypes viz., ICPH 2740 (G15), TS 3R (G10), PRG 176 (G8) and ICPL 96058 (G22). E2 (Gulbarga, Karnataka), E3 (Bidar, Karnataka) and E6 (Vikarabad, Telangana) were the most discriminating environments. Genotypes, ICPH 2740, PRG 176 and TS 3R were the best cultivars in all the environments whereas PRG 158 (G9), ICPL 87119 (G12), ICPL 20098 (G19) and ICPL 96058 (G22) were suitable across a wide range of environments. Genotypes, ICPH 2740 and PRG 176 can be recommended on a large scale to the farmers with small holdings to enhance pigeonpea productivity and improve the food security","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48985284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Naveenkumar, Vineeta Singh, P. Singh, A. Anandan
A set of 192 indica rice accessions were evaluated in two seasons to generate information on the extent of genetic variability, broad-sense heritability and genetic advance for yield and sheath blight related traits. The estimated genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) varied over the season. Largely, PCV values were relatively higher than GCV with reference to the studied parameters. However, GCV was found to be medium for per cent disease index (PDI) of 14th day, 28th day, area under the disease progress curve (AUDPC), and tiller number over the seasons. Interestingly, grain yield was found to be high. The trait plant height and panicle length had moderate heritability coupled with moderate genetic advance, whereas AUDPC was moderately heritable coupled with high genetic advance. The findings may be useful in breeding improved rice genotypes.
{"title":"Pattern of genetic variation in rice (Oryza sativa L.) population for sheath blight resistance over the seasons","authors":"R. Naveenkumar, Vineeta Singh, P. Singh, A. Anandan","doi":"10.31742/ijgpb.81.1.15","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.15","url":null,"abstract":"A set of 192 indica rice accessions were evaluated in two seasons to generate information on the extent of genetic variability, broad-sense heritability and genetic advance for yield and sheath blight related traits. The estimated genotypic coefficient of variation (GCV) and phenotypic coefficient of variation (PCV) varied over the season. Largely, PCV values were relatively higher than GCV with reference to the studied parameters. However, GCV was found to be medium for per cent disease index (PDI) of 14th day, 28th day, area under the disease progress curve (AUDPC), and tiller number over the seasons. Interestingly, grain yield was found to be high. The trait plant height and panicle length had moderate heritability coupled with moderate genetic advance, whereas AUDPC was moderately heritable coupled with high genetic advance. The findings may be useful in breeding improved rice genotypes.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69853533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
V. Thakur, Revathi Ponnuswamy, A. Singh, V. Shankar, Srinivasa D. Chary
The process of screening for fertility restoration trait involves test crossing with a set of cytoplasmic male sterile (CMS) lines and evaluation of F1 hybrids for pollen and spikelet fertility. In the present study, F2 mapping population derived from a cross, APMS 6A × RP 5933-123 was utilized to map Rf genes. The F2 population was also genetically analysed for pollen and spikelet fertility percentage. Chisquare (?2) analysis to showed that the fertility restoration trait followed expected digenic ratio. By bulk segregant analysis (BSA) likely Rf genes containing regions were located on chromosome 10. The SSR markers viz., RM304, RM258 located on chromosome 10 and RM23958 located on chromosome 9 showed clear polymorphism between two groups of fertile and sterile bulks. Based on BSA linkage analysis and F2 population, pollen and spikelet fertility analysis along with molecular screening results of Rf linked markers, it is concluded that Rf4 gene located on chromosome 10 is playing major role and contributing to 90% of fertility restoration trait of newly derived restorer line RP5933 along with minor effect genes from chromosome 9. The findings may be useful for rice hybrid breeding.
{"title":"Molecular tagging of Rf genes for the fertility restoration of WA-CMS system by bulk segregant analysis in rice","authors":"V. Thakur, Revathi Ponnuswamy, A. Singh, V. Shankar, Srinivasa D. Chary","doi":"10.31742/ijgpb.81.1.4","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.4","url":null,"abstract":"The process of screening for fertility restoration trait involves test crossing with a set of cytoplasmic male sterile (CMS) lines and evaluation of F1 hybrids for pollen and spikelet fertility. In the present study, F2 mapping population derived from a cross, APMS 6A × RP 5933-123 was utilized to map Rf genes. The F2 population was also genetically analysed for pollen and spikelet fertility percentage. Chisquare (?2) analysis to showed that the fertility restoration trait followed expected digenic ratio. By bulk segregant analysis (BSA) likely Rf genes containing regions were located on chromosome 10. The SSR markers viz., RM304, RM258 located on chromosome 10 and RM23958 located on chromosome 9 showed clear polymorphism between two groups of fertile and sterile bulks. Based on BSA linkage analysis and F2 population, pollen and spikelet fertility analysis along with molecular screening results of Rf linked markers, it is concluded that Rf4 gene located on chromosome 10 is playing major role and contributing to 90% of fertility restoration trait of newly derived restorer line RP5933 along with minor effect genes from chromosome 9. The findings may be useful for rice hybrid breeding.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47184711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prem Chand Gyani, H. Bollinedi, S. G. Krishnan, K. Vinod, P. Bhowmick, M. Nagarajan, N. Singh, R. Ellur, Ashok K. Singh
Phytic acid (PA) acts as chelator of cationic mineral elements iron (Fe) and zinc (Zn) and obstructs their absorption in the human gut. We have evaluated a set of 162 traditional rice landraces for phytic acid phosphorus (PA-P), inorganic phosphorus (Pi) and total phosphorus (TP). Wide variability was observed for PA-P, Pi and TP ranging from 1.12 to 3 mg/g, 0.004 to 0.16 mg/g and 1.17 to 3.04 mg/g respectively. The mineral micronutrients, Fe and Zn were not in correlation with PA-P, Pi and TP implicating the possibility of their independent improvement while PAP showed a significant positive correlation with TP and significant negative correlation with Pi. Principal component analysis (PCA) identified two principal components PC1 and PC2, explaining 50.6 % and 32.1 % of the total variation, respectively. Cluster analysis grouped the accessions into four clusters. The study has also led to the identification of promising donors such as P1490 and Gowri with low PA content to be utilized in rice biofortification programmes.
{"title":"Characterizing the diversity of grain phytic acid content in Indian rice landraces by multivariate genetic analyses","authors":"Prem Chand Gyani, H. Bollinedi, S. G. Krishnan, K. Vinod, P. Bhowmick, M. Nagarajan, N. Singh, R. Ellur, Ashok K. Singh","doi":"10.31742/ijgpb.81.1.3","DOIUrl":"https://doi.org/10.31742/ijgpb.81.1.3","url":null,"abstract":"Phytic acid (PA) acts as chelator of cationic mineral elements iron (Fe) and zinc (Zn) and obstructs their absorption in the human gut. We have evaluated a set of 162 traditional rice landraces for phytic acid phosphorus (PA-P), inorganic phosphorus (Pi) and total phosphorus (TP). Wide variability was observed for PA-P, Pi and TP ranging from 1.12 to 3 mg/g, 0.004 to 0.16 mg/g and 1.17 to 3.04 mg/g respectively. The mineral micronutrients, Fe and Zn were not in correlation with PA-P, Pi and TP implicating the possibility of their independent improvement while PAP showed a significant positive correlation with TP and significant negative correlation with Pi. Principal component analysis (PCA) identified two principal components PC1 and PC2, explaining 50.6 % and 32.1 % of the total variation, respectively. Cluster analysis grouped the accessions into four clusters. The study has also led to the identification of promising donors such as P1490 and Gowri with low PA content to be utilized in rice biofortification programmes.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46814272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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