W. Philanim, Ajay Kumar, Nivedita . Shittegar, S. Sankar, C. Bharadwaj, U. Ngangkham, B. Bhattacharjee
A study on stability analysis was carried out in 30 diverse genotypes of ricebean (Vigna umbellata Thunb.) in eastern hill region of India. Significant differences among the genotypes over the years were observed for all the three traits studied. The first two principal components axis (IPCA1, 90.4% and IPCA2, 9.6%) could explain 100% of the total of the interaction variation. Correlation study showed that the stability parameters are highly associated with each other (p < 0.01). Multi trait stability index and genotype selection along with strength and weakness view of the genotypes to the traits inferred higher productivity of BSKB 28 (G29) for number of pods/plant and seed yield/plant and Ukhrul 15 (G17) for number of seeds/pod. Different stability measures selected seven genotypes, namely, Bete 6 (G22), IC002567 (G8), Ukhrul 6 (G13), Ukhrul 14 (G16), Ukhrul 15 (G17), Bete 4 (G21) and BSKB 3 (G28) as the most desirable and stable performing with good yield over the years.
{"title":"Stability analysis of yield and yield related traits in ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi]","authors":"W. Philanim, Ajay Kumar, Nivedita . Shittegar, S. Sankar, C. Bharadwaj, U. Ngangkham, B. Bhattacharjee","doi":"10.31742/ijgpb.82.2.10","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.10","url":null,"abstract":"A study on stability analysis was carried out in 30 diverse genotypes of ricebean (Vigna umbellata Thunb.) in eastern hill region of India. Significant differences among the genotypes over the years were observed for all the three traits studied. The first two principal components axis (IPCA1, 90.4% and IPCA2, 9.6%) could explain 100% of the total of the interaction variation. Correlation study showed that the stability parameters are highly associated with each other (p < 0.01). Multi trait stability index and genotype selection along with strength and weakness view of the genotypes to the traits inferred higher productivity of BSKB 28 (G29) for number of pods/plant and seed yield/plant and Ukhrul 15 (G17) for number of seeds/pod. Different stability measures selected seven genotypes, namely, Bete 6 (G22), IC002567 (G8), Ukhrul 6 (G13), Ukhrul 14 (G16), Ukhrul 15 (G17), Bete 4 (G21) and BSKB 3 (G28) as the most desirable and stable performing with good yield over the years.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43345015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rajat Chaudhary, Y. ., Priya Pardeshi, J. Nanjundan, S. Yadav, S. Vasudev, D. K. Yadava, N. Saini
Intron Polymorphism (IP) markers were used to unravel the genetic variation and relationship among 26 genotypes representing six cultivated Brassica species described in the classical U triangle. One hundred and twenty-five Arabidopsis thaliana-derived IP markers were assayed and 90 to 100% cross-transferability was observed in the six Brassica species suggesting that IP markers were highly conserved during the evolution of different Brassica species. The number of alleles observed in species at each locus ranged from one to ten with an average of 2.89 alleles per primer pair and there was no consensus between the number of alleles amplified in diploid and tetraploid species. The size range of amplified alleles was 120-1250bp, which reflects enormous deletions/insertions in different alleles. In B. juncea, 100% cross-transferability had been obtained and 121 IP markers resulted in polymorphic amplicons with PIC value of 0.04 to 0.48. The dendrogram divided all the 26 genotypes into two groups composed of B. napus/B. rapa/B. oleracea and B. carinata/B. nigra/B. juncea. A-genome present in B. juncea and B. napus/B. rapa seems distinct from each other and hence provides a great opportunity for generating diversity through resynthesizing amphidiploids from different available sources of Agenome. The A and B genomes are more similar in comparison to C genome in tetra-diploid species.The evolutionary relationship established between various Brassica species would support in formulating suitable breeding approaches for widening the genetic base of Brassica amphidiploids by exploiting the genetic diversity found in diploid progenitor gene pools.
{"title":"Relationship and genetic diversity analysis of Brassica juncea and U tringle species using intron polymorphic markers","authors":"Rajat Chaudhary, Y. ., Priya Pardeshi, J. Nanjundan, S. Yadav, S. Vasudev, D. K. Yadava, N. Saini","doi":"10.31742/ijgpb.82.2.6","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.6","url":null,"abstract":"Intron Polymorphism (IP) markers were used to unravel the genetic variation and relationship among 26 genotypes representing six cultivated Brassica species described in the classical U triangle. One hundred and twenty-five Arabidopsis thaliana-derived IP markers were assayed and 90 to 100% cross-transferability was observed in the six Brassica species suggesting that IP markers were highly conserved during the evolution of different Brassica species. The number of alleles observed in species at each locus ranged from one to ten with an average of 2.89 alleles per primer pair and there was no consensus between the number of alleles amplified in diploid and tetraploid species. The size range of amplified alleles was 120-1250bp, which reflects enormous deletions/insertions in different alleles. In B. juncea, 100% cross-transferability had been obtained and 121 IP markers resulted in polymorphic amplicons with PIC value of 0.04 to 0.48. The dendrogram divided all the 26 genotypes into two groups composed of B. napus/B. rapa/B. oleracea and B. carinata/B. nigra/B. juncea. A-genome present in B. juncea and B. napus/B. rapa seems distinct from each other and hence provides a great opportunity for generating diversity through resynthesizing amphidiploids from different available sources of Agenome. The A and B genomes are more similar in comparison to C genome in tetra-diploid species.The evolutionary relationship established between various Brassica species would support in formulating suitable breeding approaches for widening the genetic base of Brassica amphidiploids by exploiting the genetic diversity found in diploid progenitor gene pools.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48515190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pardeep Kumar, Ningthaipuilu Longmei, B. S. Jat, M. Choudhary, K. R. Yathish, B. Bhushan, M. Goyal, S. Rakshit
The present study involved estimating the combining ability effects and heterotic grouping to increase the efficacy of parental selection for successful hybrid development. A total of 52 F1 hybrids were generated by Line × Tester mating design by crossing 26 diverse maize inbred lines with two testers, namely, HKI 323 and HKI 1105. The 26 baby corn inbred lines were classified into a different heterotic group based on the specific combining ability (SCA) effects and heterotic groups specific and general combining ability (HSGCA). The SCA effects and HSGCA of baby corn yield without husk (BCY) classified the inbred lines into five and four groups, respectively. This is the first study of heterotic’s grouping for baby corn germplasm considering BCY based on SCA and HSGCA methods. The information generated through heterotic grouping may be used to derive new inbred lines and develop different heterotic combinations for further utilization in breeding programs.
{"title":"Heterotic grouping of Indian baby corn lines based on combining ability","authors":"Pardeep Kumar, Ningthaipuilu Longmei, B. S. Jat, M. Choudhary, K. R. Yathish, B. Bhushan, M. Goyal, S. Rakshit","doi":"10.31742/ijgpb.82.2.4","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.4","url":null,"abstract":"The present study involved estimating the combining ability effects and heterotic grouping to increase the efficacy of parental selection for successful hybrid development. A total of 52 F1 hybrids were generated by Line × Tester mating design by crossing 26 diverse maize inbred lines with two testers, namely, HKI 323 and HKI 1105. The 26 baby corn inbred lines were classified into a different heterotic group based on the specific combining ability (SCA) effects and heterotic groups specific and general combining ability (HSGCA). The SCA effects and HSGCA of baby corn yield without husk (BCY) classified the inbred lines into five and four groups, respectively. This is the first study of heterotic’s grouping for baby corn germplasm considering BCY based on SCA and HSGCA methods. The information generated through heterotic grouping may be used to derive new inbred lines and develop different heterotic combinations for further utilization in breeding programs.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43875968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Highly significant effects of genotypes yield had been observed during field evaluation. A total variation of 51% accounted by the environments, 32.9% by genotype x environment interaction, and only 10.7% due to the genotypes in AMMI analysis. Adaptability measure harmonic means of relative performance of genotypes ranked HB13-48, HB13-10 and HB13-11 as per their performance. The relative performances of genetic values were settled for HB13-48, HB13-10, HB13-26 genotypes. ASV1 and ASV measures pointed HB13-26, HB13-15 and HB13-40 as of stable performance. Biplot analysis observed the strong bondage of arithmetic, geometric and harmonic means along with relative performance of genetic values and measure of the relative performance of the genotypic values. The information accrued from the study may be useful in cultivating stable genotype(s).
{"title":"Long-term evaluation of new elite genotypes of Faba bean (Vicia faba L.) by Superiority Index and AMMI analysis","authors":"R. Arya, A. Verma, V. .","doi":"10.31742/ijgpb.82.2.14","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.14","url":null,"abstract":"Highly significant effects of genotypes yield had been observed during field evaluation. A total variation of 51% accounted by the environments, 32.9% by genotype x environment interaction, and only 10.7% due to the genotypes in AMMI analysis. Adaptability measure harmonic means of relative performance of genotypes ranked HB13-48, HB13-10 and HB13-11 as per their performance. The relative performances of genetic values were settled for HB13-48, HB13-10, HB13-26 genotypes. ASV1 and ASV measures pointed HB13-26, HB13-15 and HB13-40 as of stable performance. Biplot analysis observed the strong bondage of arithmetic, geometric and harmonic means along with relative performance of genetic values and measure of the relative performance of the genotypic values. The information accrued from the study may be useful in cultivating stable genotype(s).","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45523363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Basavaraj, C. Gireesh, Muralidhara Bharamappanavara, C. Manoj, Lakshmi V. G. Ishwaryalakshmi, P. Senguttuve, R. Sundaram, L. Subbarao, M. Anantha
Soil phosphorus (P) deficiency is one of the major challenges for rice cultivation. The present study was carried out under low soil P condition (available P, < 2 kg ha-1) to study the genetic variability, correlation, path and principal component analysis (PCA) in a set of 40 introgression lines derived from Samba Mahsuri (Oryza sativa ssp. indica) × Oryza rufipogon. High genotypic coefficient of variation and phenotypic coefficient of variation was recorded for productive tillers (PT), seed weight (SW), and grain yield (GY). High heritability coupled with high genetic advance was recorded for plant height (PH), PT, SW, number of spikelets/panicle (SPP), spikelet fertility, and GY. Correlation analysis revealed that grain yield had a significant positive association with PH, PT, panicle length, SPP, and SW. Regression analysis revealed that the grain yield was mainly influenced by PT and SW. This was confirmed by path analysis, wherein PT and SW exerted the highest direct positive effect on grain yield. The PCA revealed that the first two PCs contribute to maximum variability, which together accounted for 60% of the total variability. The traits PT and SW contributed the maximum to load/variance the total variability. Thus, the selection of introgression lines with a greater number of PT and higher SW would be the most appropriate strategy for yield improvement under low soil P condition in rice. Cluster analysis grouped introgression lines along with checks into three clusters. Overall, the study revealed the pattern genetic variability for low P tolerance in the introgression lines. This further helps in designing appropriate breeding strategies for the improvement of rice cultivars for low P tolerance.
{"title":"Genetic analysis of introgression lines of Oryza rufipogon for improvement of low phosphorous tolerance in indica rice","authors":"P. Basavaraj, C. Gireesh, Muralidhara Bharamappanavara, C. Manoj, Lakshmi V. G. Ishwaryalakshmi, P. Senguttuve, R. Sundaram, L. Subbarao, M. Anantha","doi":"10.31742/ijgpb.82.2.1","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.1","url":null,"abstract":"Soil phosphorus (P) deficiency is one of the major challenges for rice cultivation. The present study was carried out under low soil P condition (available P, < 2 kg ha-1) to study the genetic variability, correlation, path and principal component analysis (PCA) in a set of 40 introgression lines derived from Samba Mahsuri (Oryza sativa ssp. indica) × Oryza rufipogon. High genotypic coefficient of variation and phenotypic coefficient of variation was recorded for productive tillers (PT), seed weight (SW), and grain yield (GY). High heritability coupled with high genetic advance was recorded for plant height (PH), PT, SW, number of spikelets/panicle (SPP), spikelet fertility, and GY. Correlation analysis revealed that grain yield had a significant positive association with PH, PT, panicle length, SPP, and SW. Regression analysis revealed that the grain yield was mainly influenced by PT and SW. This was confirmed by path analysis, wherein PT and SW exerted the highest direct positive effect on grain yield. The PCA revealed that the first two PCs contribute to maximum variability, which together accounted for 60% of the total variability. The traits PT and SW contributed the maximum to load/variance the total variability. Thus, the selection of introgression lines with a greater number of PT and higher SW would be the most appropriate strategy for yield improvement under low soil P condition in rice. Cluster analysis grouped introgression lines along with checks into three clusters. Overall, the study revealed the pattern genetic variability for low P tolerance in the introgression lines. This further helps in designing appropriate breeding strategies for the improvement of rice cultivars for low P tolerance.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42957376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Sinto, L. Sathee, D. Singh, S. Jha, Sandeep B. Adavi, R. Kumar, V. Chinnusamy, Madanpal Singh
The rise in atmospheric CO2 levels impacts humankind by threatening food and nutritional security. The strong correlation between crop yield and grain weight in cereals is an essential component of yield stability. Further, improving grain protein and mineral nutrient content is a crucial breeding target for cereal crops. The study was performed to understand the interactive effects of elevated CO2 (EC) and nitrogen (N) fertilization on grain ionome, grain yield parameters, grain morphology, and the expression of genes related to grain morphology. The changes in ionome and grain parameters were examined in response to two N levels optimal N (ON: 500 mg/pot) and high N (HN: 625 mg/pot) along with atmospheric CO2 enrichment [ambient (CO2) of 400 ±10 ppm and elevated (CO2) of 700 ±10 ppm]. Grain ionome (N, K, Ca and Fe) showed a general decrease in EC-grown wheat plants. The expression of genes related to grain length (TaGL3 and TaGL7) were upregulated, and those genes related to grain width (TaGW2 and TaGW6) were downregulated under EC in maturing spikelet of wheat. In the case of TaSnRK2, the expression was promoted by EC in HN treatment. The complex regulation of source and sink-associated gene transcript abundance indicates an EC mediated alteration in N and sugar signalling in wheat.
{"title":"Elevated CO2 and Nitrogen dose affect grain ionome, grain morphology and associated gene expression in wheat (Triticum aestivum L.)","authors":"A. Sinto, L. Sathee, D. Singh, S. Jha, Sandeep B. Adavi, R. Kumar, V. Chinnusamy, Madanpal Singh","doi":"10.31742/ijgpb.82.2.2","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.2","url":null,"abstract":"The rise in atmospheric CO2 levels impacts humankind by threatening food and nutritional security. The strong correlation between crop yield and grain weight in cereals is an essential component of yield stability. Further, improving grain protein and mineral nutrient content is a crucial breeding target for cereal crops. The study was performed to understand the interactive effects of elevated CO2 (EC) and nitrogen (N) fertilization on grain ionome, grain yield parameters, grain morphology, and the expression of genes related to grain morphology. The changes in ionome and grain parameters were examined in response to two N levels optimal N (ON: 500 mg/pot) and high N (HN: 625 mg/pot) along with atmospheric CO2 enrichment [ambient (CO2) of 400 ±10 ppm and elevated (CO2) of 700 ±10 ppm]. Grain ionome (N, K, Ca and Fe) showed a general decrease in EC-grown wheat plants. The expression of genes related to grain length (TaGL3 and TaGL7) were upregulated, and those genes related to grain width (TaGW2 and TaGW6) were downregulated under EC in maturing spikelet of wheat. In the case of TaSnRK2, the expression was promoted by EC in HN treatment. The complex regulation of source and sink-associated gene transcript abundance indicates an EC mediated alteration in N and sugar signalling in wheat.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69853781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Micronuclei (MN) are extra-nuclear bodies containing damaged chromosomal fragments as well as complete chromosomes that are not otherwise incorporated into the nucleus after cell division. The present experiment was conducted to assess mutagenic effects of ethyl methane sulphonate (EMS) on the meiotic behavior of pollen mother cells of fennel (Foeniculum vulgare Mill.). Cytological analysis revealed that micronuclei were incited at all the three concentrations of EMS. A peculiar correlation of chromosomal abnormalities, mainly bridge and laggard at anaphase I and II with this nuclear polymorphism were experienced for genomic instability. Pollen viability was additionally inspected through pollen fertility. Thus, the MN formations bring about incomplete elimination of the genome, which can be proficiently use as the light of hope inbreeding programmers to produce addition and substitution lines.
{"title":"Emergence of micronuclei as polymorphism induced genomic instability in Fennel (Foeniculum vulgare Mill.).","authors":"G. Kumar, M. Mishra, K. Tripathi, P. Ojha","doi":"10.31742/ijgpb.82.2.16","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.16","url":null,"abstract":"Micronuclei (MN) are extra-nuclear bodies containing damaged chromosomal fragments as well as complete chromosomes that are not otherwise incorporated into the nucleus after cell division. The present experiment was conducted to assess mutagenic effects of ethyl methane sulphonate (EMS) on the meiotic behavior of pollen mother cells of fennel (Foeniculum vulgare Mill.). Cytological analysis revealed that micronuclei were incited at all the three concentrations of EMS. A peculiar correlation of chromosomal abnormalities, mainly bridge and laggard at anaphase I and II with this nuclear polymorphism were experienced for genomic instability. Pollen viability was additionally inspected through pollen fertility. Thus, the MN formations bring about incomplete elimination of the genome, which can be proficiently use as the light of hope inbreeding programmers to produce addition and substitution lines.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41628146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Increasing temperatures are becoming a major challenge for agro-systems. Late mustard planting often results in overall growth reduction, poor metabolism, less productivity and yield due to heat stress at the terminal stage. A set of 45 genotypes comprising introgression lines, landraces, wild species, induced mutants, advance breeding lines and the released varieties/cultivars were evaluated under normal (Yp) and stressed environment (Ys) for yield stability during 2016-2018. The higher value of heat tolerance index, geometric mean productivity, yield stability index, relative stability index and yield production score index with lower heat susceptibility index indicated tolerance to high temperature in some genotypes. Based on the yield performance and derived traits, six genotypes namely, HLM-41-13-2 and ELM-38 with zero erucic acid quality, MCN-05-8 an induced mutant, germplasm lines PCR-3 and CSR-1163, while MCN-08-2 is released variety RB50 were rated as promising for heat stress tolerance at both the locations. Biplot analysis indicated that genotypes under group I and IV are tolerant of high yield potential and stability in a stressed environment irrespective of location.
{"title":"Study on heat stress indices and their correlation with yield in Indian mustard genotypes under diverse conditions","authors":"Vijay Kamal Meena","doi":"10.31742/ijgpb.82.2.7","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.7","url":null,"abstract":"Increasing temperatures are becoming a major challenge for agro-systems. Late mustard planting often results in overall growth reduction, poor metabolism, less productivity and yield due to heat stress at the terminal stage. A set of 45 genotypes comprising introgression lines, landraces, wild species, induced mutants, advance breeding lines and the released varieties/cultivars were evaluated under normal (Yp) and stressed environment (Ys) for yield stability during 2016-2018. The higher value of heat tolerance index, geometric mean productivity, yield stability index, relative stability index and yield production score index with lower heat susceptibility index indicated tolerance to high temperature in some genotypes. Based on the yield performance and derived traits, six genotypes namely, HLM-41-13-2 and ELM-38 with zero erucic acid quality, MCN-05-8 an induced mutant, germplasm lines PCR-3 and CSR-1163, while MCN-08-2 is released variety RB50 were rated as promising for heat stress tolerance at both the locations. Biplot analysis indicated that genotypes under group I and IV are tolerant of high yield potential and stability in a stressed environment irrespective of location.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42542144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sudarshana Borah, Banashree Saikia, D. Bora, P. Bhorali
The Sigatoka disease is an economically important disease of banana as it causes tremendous yield losses all over the world. In the present study, the defense related gene expression implicated in an incompatible interaction between a resistant banana cultivar Kachkal and the Sigatoka pathogen was analyzed. The initial changes in the expression of some selected defense related genes after infection by the invading pathogen Pseudocercospora musae were studied. Semi-quantitative RT-PCR studies indicated a basal level of expression of the selected genes prior to infection in the host plant, while upregulation of some of the important defense response genes coding for PR4, osmotin, LTP, UGPase and JAR1 was observed after the infection by P. musae. The findings of the study would be useful in designing a precise breeding strategy for genetic control of the disease in banana.
{"title":"Expression analysis of defense response genes upon Sigatoka infection in Musa paradisiaca cultivar Kachkal","authors":"Sudarshana Borah, Banashree Saikia, D. Bora, P. Bhorali","doi":"10.31742/ijgpb.82.2.11","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.11","url":null,"abstract":"The Sigatoka disease is an economically important disease of banana as it causes tremendous yield losses all over the world. In the present study, the defense related gene expression implicated in an incompatible interaction between a resistant banana cultivar Kachkal and the Sigatoka pathogen was analyzed. The initial changes in the expression of some selected defense related genes after infection by the invading pathogen Pseudocercospora musae were studied. Semi-quantitative RT-PCR studies indicated a basal level of expression of the selected genes prior to infection in the host plant, while upregulation of some of the important defense response genes coding for PR4, osmotin, LTP, UGPase and JAR1 was observed after the infection by P. musae. The findings of the study would be useful in designing a precise breeding strategy for genetic control of the disease in banana.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43808491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. K. Azameti, Pankaj Singh, K. Gaikwad, M. Dalal, A. Arora, V. Rai, J. Padaria
Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42oC for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.
{"title":"Isolation and characterization of novel gene TaSSRP differentially expressed in wheat (Triticum aestivum L.) genotypes under heat stress","authors":"M. K. Azameti, Pankaj Singh, K. Gaikwad, M. Dalal, A. Arora, V. Rai, J. Padaria","doi":"10.31742/ijgpb.82.2.12","DOIUrl":"https://doi.org/10.31742/ijgpb.82.2.12","url":null,"abstract":"Analysis of heat stress-responsive transcriptome data in wheat genotype Raj 3765 revealed that expression of salt stress root protein (SSRP) RS1 was observed to increase by 8.29 folds in the flag leaf at the grain filling stage. Further validation of the gene under heat stress condition (42oC for 6 hours) by qRT-PCR revealed an increase of 8.42 folds. In-silico studies revealed that the gene is localized mainly in the cytoplasm, and did not contain transmembrane helix. The presence of 15 phosphorylation sites as indicated by in-silico analysis could play significant roles in the heat stress tolerance.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45196615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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