Background: Feed is one of the most important parts of the livestock production system because it can account for 60-80% of production costs, besides that very elements needed by livestock to grow, develop and produce. A study has been conducted to evaluate the effects of chemical and biological urea-treated durian husk on the production performance of Kacang female goats. Methods: The research method used was plotted in the 2 ´ 3 factorial randomized block design (RBD). The first factor was two processing methods of urease (A) and fermentation (F) and the second factor was three levels of urea, namely 1.0% (L1), 2.5% (L2) and 5% (L3), with four replicates as groups. The data were analyzed using analysis of variances and mean comparison using the least significant difference (LSD) test. Result: The results showed however, the application of durian husk with different urea levels produced a significant effect (P less than 0.05) on the body weight gain, hematocrit value, non-carcass weight and percentage, internal non-carcass weight and percentage, as well as non-carcass weight and percentage, of Kacang female goats. That administration of chemically and biologically processed durian fruit husk supplemented with urea up to 5.0%, can be implemented without negative effects on production performance, physiological conditions, carcass characteristics, as well as non-carcass characteristics.
{"title":"Performance of Kacang Goats Fed Chemically and Biologically Treated Durian Husk","authors":"Fatmawati Fatmawati, Padang Hamid, Rusdi Rusdi, Naharuddin Naharuddin","doi":"10.18805/ijar.bf-1701","DOIUrl":"https://doi.org/10.18805/ijar.bf-1701","url":null,"abstract":"Background: Feed is one of the most important parts of the livestock production system because it can account for 60-80% of production costs, besides that very elements needed by livestock to grow, develop and produce. A study has been conducted to evaluate the effects of chemical and biological urea-treated durian husk on the production performance of Kacang female goats. Methods: The research method used was plotted in the 2 ´ 3 factorial randomized block design (RBD). The first factor was two processing methods of urease (A) and fermentation (F) and the second factor was three levels of urea, namely 1.0% (L1), 2.5% (L2) and 5% (L3), with four replicates as groups. The data were analyzed using analysis of variances and mean comparison using the least significant difference (LSD) test. Result: The results showed however, the application of durian husk with different urea levels produced a significant effect (P less than 0.05) on the body weight gain, hematocrit value, non-carcass weight and percentage, internal non-carcass weight and percentage, as well as non-carcass weight and percentage, of Kacang female goats. That administration of chemically and biologically processed durian fruit husk supplemented with urea up to 5.0%, can be implemented without negative effects on production performance, physiological conditions, carcass characteristics, as well as non-carcass characteristics.\u0000","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"10 20","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138589870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Thangadurai, P. Kohila, M. Ramasamy, G. Kumar, M. A. Vennila, K. Sivakumar
Background: The present study was undertaken to assess the probiotic supplement through feed to enhance performance of TANUVAS Aseel chicken. Methods: Three hundred one month old TANUVAS Aseel chicks were supplied to the farmers of three groups (T1, T2 and T3), (Irrespective of number of birds seven replication in each treatment) under backyard system of rearing, all the chicks were fed concentrate feed along with foraging. T1 was control, T2 was Probeads EC supplement (5 beads/day/bird/oral) and T3 was probiotic supplement (1 g per kg of body weight). Result: The average body weight, daily weight gain, feed conversion efficiently, livability, cost benefit analysis were significantly higher in T2 (Probeads EC supplement) (P less than 0.05) compared to T3 and control (T1) groups.
{"title":"Assessment of Probiotic Supplement to Enhance Performance of TANUVAS Aseel Chicken","authors":"R. Thangadurai, P. Kohila, M. Ramasamy, G. Kumar, M. A. Vennila, K. Sivakumar","doi":"10.18805/ijar.b-5245","DOIUrl":"https://doi.org/10.18805/ijar.b-5245","url":null,"abstract":"Background: The present study was undertaken to assess the probiotic supplement through feed to enhance performance of TANUVAS Aseel chicken. Methods: Three hundred one month old TANUVAS Aseel chicks were supplied to the farmers of three groups (T1, T2 and T3), (Irrespective of number of birds seven replication in each treatment) under backyard system of rearing, all the chicks were fed concentrate feed along with foraging. T1 was control, T2 was Probeads EC supplement (5 beads/day/bird/oral) and T3 was probiotic supplement (1 g per kg of body weight). Result: The average body weight, daily weight gain, feed conversion efficiently, livability, cost benefit analysis were significantly higher in T2 (Probeads EC supplement) (P less than 0.05) compared to T3 and control (T1) groups.","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"41 26","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138593753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kanagaraja Anantharaja, G. Barlaya, CH Raghavendra, BS Anand Kumar, Siddaiah Gm, N.K. Chadha, Gopal Krishna, P. Routray, K. Hemaprasanth
Background: Biofloc technology relies on adding a carbon source to maintain optimal carbon to nitrogen ratio for transforming the waste into microbial biomass that aquatic animals can use. In the present study, the growth performance of the critically endangered Hypselobarbus pulchellus in biofloc-based seed rearing with different Carbon to Nitrogen ratio was studied. Methods: Healthy early fry (0.01 g) was randomly stocked into 15 circular FRP tanks (1000 L) at a density of 100 individuals/m3 and reared for 90 days. The carbon to nitrogen ratios of 5:1(CN5), 10:1(CN10), 15:1(CN15) and 20:1(CN20) were maintained using jaggery without considering carbon content derived from the feed and the control group received no additional source of carbon. Result: The results indicated that H. pulchellus had better growth performance in biofloc systems having higher C/N ratios. The highest weight gain (0.48±0.01 g) and weight gain percentage (3931.15±120.68%) and specific growth rate (4.11±0.03% per day) were found in the CN10 group (P less than 0.05), followed by CN15 and CN20, while the control group values were lowest. In terms of survival, all treatments had high survival rates ranging from 92% to 94.67%, with no significant variations (P greater than 0.05). The CN10, CN15 and CN20 treatments had significantly higher amylase, protease and lipase activities than control (P less than 0.05). Moreover, the immunological and antioxidant activity increased with CN ratios. The CN10, CN15 and CN20 groups had significantly lower total ammonia nitrogen, nitrite and nitrate levels than control group (P less than 0.05). Overall, this study suggests that C/N ratios of 10:1 to 20:1 are optimal for rearing H. pulchellus fingerlings from early fry, with 10:1 presenting the best growth results.
{"title":"Growth Performance of Critically Endangered Hypselobarbus pulchellus in Biofloc Systems with Various C/N Ratios","authors":"Kanagaraja Anantharaja, G. Barlaya, CH Raghavendra, BS Anand Kumar, Siddaiah Gm, N.K. Chadha, Gopal Krishna, P. Routray, K. Hemaprasanth","doi":"10.18805/ijar.b-5202","DOIUrl":"https://doi.org/10.18805/ijar.b-5202","url":null,"abstract":"Background: Biofloc technology relies on adding a carbon source to maintain optimal carbon to nitrogen ratio for transforming the waste into microbial biomass that aquatic animals can use. In the present study, the growth performance of the critically endangered Hypselobarbus pulchellus in biofloc-based seed rearing with different Carbon to Nitrogen ratio was studied. Methods: Healthy early fry (0.01 g) was randomly stocked into 15 circular FRP tanks (1000 L) at a density of 100 individuals/m3 and reared for 90 days. The carbon to nitrogen ratios of 5:1(CN5), 10:1(CN10), 15:1(CN15) and 20:1(CN20) were maintained using jaggery without considering carbon content derived from the feed and the control group received no additional source of carbon. Result: The results indicated that H. pulchellus had better growth performance in biofloc systems having higher C/N ratios. The highest weight gain (0.48±0.01 g) and weight gain percentage (3931.15±120.68%) and specific growth rate (4.11±0.03% per day) were found in the CN10 group (P less than 0.05), followed by CN15 and CN20, while the control group values were lowest. In terms of survival, all treatments had high survival rates ranging from 92% to 94.67%, with no significant variations (P greater than 0.05). The CN10, CN15 and CN20 treatments had significantly higher amylase, protease and lipase activities than control (P less than 0.05). Moreover, the immunological and antioxidant activity increased with CN ratios. The CN10, CN15 and CN20 groups had significantly lower total ammonia nitrogen, nitrite and nitrate levels than control group (P less than 0.05). Overall, this study suggests that C/N ratios of 10:1 to 20:1 are optimal for rearing H. pulchellus fingerlings from early fry, with 10:1 presenting the best growth results.\u0000","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"37 11","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138594098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mares, M. Murshed, Hossam M A Aljawdah, Waleed Ali Hailan, S. Al-Quraishy
Background: Echinococcus granulosus is a type of taeniid tapeworm, present in the small intestine of its primary hosts, including dogs and occasionally in other carnivores. The larval stage of E. granulosus causes cystic hydatidosis in humans and livestock. Although most hydatid cysts are found in the liver and lungs, the disease can occur anywhere in the body. Therefore, Hydatidosis remains a problem and causes significant economic losses in animals. Methods: A study was performed to evaluate the activity of the leaves Nerium oleander extract on the viability of protoscolices. We used three graduated concentrations of extract (10, 30 and 50 mg/ml). 2 ml of the protoscolices suspension (containing at least 2700 protoscolices) was placed in test tubes using a Pasteur pipette. Then, 2 ml of various concentrations of the Nerium oleander extract were added to each test tube and mixed gently, incubated at 37°C for 5, 10, 20 and 30 min. One control group was kept that contain normal saline. The viability of protoscolices was evaluated by eosin method where, protoscolices suspension was mixed with 0.1% eosin for 5 minutes. The dead protoscolices absorb eosin and becomes red, whereas the live protoscolices remains colorless. Before drawing the conclusion, each experiment was performed thrice. Result: The analysis of phytochemicals by FT-IR for alcoholic extracts of Nerium oleander extracts revealed the presence of 12 effective chemical ingredients. Nerium oleander extract showed the strongest scolicidal effect (85.6, 92.3%) after 20 and 30 min at a concentration of 50 mg/ml, respectively moderately (69.3%) after 30 min at 30 mg/ml and lowest (41.9%) after 30 min at 10 mg/ml. Microscopic examinations of dead protoscolices showed distortion of their morphology and degenerative effects. These effects were characterized by loss of hooks, or presence of free hooks and dissolution and rupture of the protoscolices wall. It can be concluded from this study that ethanol extract of Nerium oleander is effective against Protoscolies in vitro.
{"title":"Effectiveness Evaluation of Leaves Nerium oleander Extract on the Viability of Protoscolices: In vitro","authors":"M. Mares, M. Murshed, Hossam M A Aljawdah, Waleed Ali Hailan, S. Al-Quraishy","doi":"10.18805/ijar.bf-1702","DOIUrl":"https://doi.org/10.18805/ijar.bf-1702","url":null,"abstract":"Background: Echinococcus granulosus is a type of taeniid tapeworm, present in the small intestine of its primary hosts, including dogs and occasionally in other carnivores. The larval stage of E. granulosus causes cystic hydatidosis in humans and livestock. Although most hydatid cysts are found in the liver and lungs, the disease can occur anywhere in the body. Therefore, Hydatidosis remains a problem and causes significant economic losses in animals. Methods: A study was performed to evaluate the activity of the leaves Nerium oleander extract on the viability of protoscolices. We used three graduated concentrations of extract (10, 30 and 50 mg/ml). 2 ml of the protoscolices suspension (containing at least 2700 protoscolices) was placed in test tubes using a Pasteur pipette. Then, 2 ml of various concentrations of the Nerium oleander extract were added to each test tube and mixed gently, incubated at 37°C for 5, 10, 20 and 30 min. One control group was kept that contain normal saline. The viability of protoscolices was evaluated by eosin method where, protoscolices suspension was mixed with 0.1% eosin for 5 minutes. The dead protoscolices absorb eosin and becomes red, whereas the live protoscolices remains colorless. Before drawing the conclusion, each experiment was performed thrice. Result: The analysis of phytochemicals by FT-IR for alcoholic extracts of Nerium oleander extracts revealed the presence of 12 effective chemical ingredients. Nerium oleander extract showed the strongest scolicidal effect (85.6, 92.3%) after 20 and 30 min at a concentration of 50 mg/ml, respectively moderately (69.3%) after 30 min at 30 mg/ml and lowest (41.9%) after 30 min at 10 mg/ml. Microscopic examinations of dead protoscolices showed distortion of their morphology and degenerative effects. These effects were characterized by loss of hooks, or presence of free hooks and dissolution and rupture of the protoscolices wall. It can be concluded from this study that ethanol extract of Nerium oleander is effective against Protoscolies in vitro.\u0000","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"99 7","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138590679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Saponins have been considered to affect the digestion of animal because of its relatively complex compound. Regarding the effect of saponins on animal production performance, different animals or the same animals with different physiological periods are different. The purpose of this experiment was to evaluate the effect of dietary alfalfa saponins (AS) on the digestive physiology of piglets. Methods: 72 weaned piglets (Duroc × Landrace × Large white pigs) were weaned at 28 days of age with an average weight of 7.5 kg and assigned randomly to 4 treatment groups. Diets containing 0, 0.5%, 1%, 2% alfalfa saponins were fed to piglets. Result: The results showed that: Alfalfa saponins increased daily gain and decreased diarrhea in piglets to some extent. It tended to decrease the pH value of the gastrointestinal tract and increase the pH value of the jejunum (P less than 0.05), ileum and cecum (P less than 0.05). AS had no obvious inhibitory effect on Escherichia coli, but had an increasing trend on the number of intestine Lactobacillus, especially Lactobacillus in jejunum increased significantly by adding 2% AS (P less than 0.05). Adding 2% alfalfa saponins can improve the digestive physiology of piglets.
{"title":"Effects of Dietary Alfalfa Saponin on Digestive Physiology in Weaned Piglets","authors":"W.N. Fan, Y.X. Yang, Y.Q. Shi, C.Z. Wang, B. Xu","doi":"10.18805/ijar.bf-1686","DOIUrl":"https://doi.org/10.18805/ijar.bf-1686","url":null,"abstract":"Background: Saponins have been considered to affect the digestion of animal because of its relatively complex compound. Regarding the effect of saponins on animal production performance, different animals or the same animals with different physiological periods are different. The purpose of this experiment was to evaluate the effect of dietary alfalfa saponins (AS) on the digestive physiology of piglets. Methods: 72 weaned piglets (Duroc × Landrace × Large white pigs) were weaned at 28 days of age with an average weight of 7.5 kg and assigned randomly to 4 treatment groups. Diets containing 0, 0.5%, 1%, 2% alfalfa saponins were fed to piglets. Result: The results showed that: Alfalfa saponins increased daily gain and decreased diarrhea in piglets to some extent. It tended to decrease the pH value of the gastrointestinal tract and increase the pH value of the jejunum (P less than 0.05), ileum and cecum (P less than 0.05). AS had no obvious inhibitory effect on Escherichia coli, but had an increasing trend on the number of intestine Lactobacillus, especially Lactobacillus in jejunum increased significantly by adding 2% AS (P less than 0.05). Adding 2% alfalfa saponins can improve the digestive physiology of piglets.","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"18 11","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138591389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. M. Saleh, A.G. Rewaida, A. Afaf, A.A. Sarah, Al-Q. Saleh, M.A. Esam
Background: The present investigation was designed to investigate the protective effect of a natural product to control Anthelmintic in earthworm Eisenia fetida. We investigated the effectiveness of dried Artemisia monosperma leaves as an anthelmintic. Methods: Five groups (50, 100, 200 and mg/mL) of the AMLE extract were utilized in this study. Negative and positive controls consisted of distilled water and mebendazole (10 mg/ml). Five worms that were around the same size were placed to each Petri plate along with the test solution (20 mL) of the extract. Artemisia monosperma leaves methanolic extract was prepared and evaluated its ability in vitro as anthelmintic activity against earthworms (Eisenia fetida). The ABTS, FRPA and DPPH techniques were used to calculate the antioxidant activity. Also, the phytochemical investigation of methanolic extract was performed on GC-MS equipment. Result: Fourteen different biomolecules are predicted using GC-MS spectrum. Also, the AMLE had a greater percentage value of ABTS, FRPA and DPPH radical inhibition (94.5±0.03 trolox/gFW, 0.8±0.005 µmol/ gFW and 92.6±0.3%) respectively. Moreover, IC50 of AMLE was obtained at 20.5±0.9 ìg/mL for the A549 and 24.3±0.9 ìg/mL for MCF-7 cell lines. In comparison to AMLE, Mebendazole (10 mg/mL) produced paralysis and earthworm death by 13.91±0.007 and 18.2±0.980 min, respectively, while, AMLE (200 mg/mL) showed 3.860±0.320 and 5.464±0.422 min, respectively. The treated worms’ surface architecture also had noticeable abnormalities, according to the histological study. The result of present study revealed that AMLE leaves can serve as a good natural source of potent antioxidants and anthelminthiasis medicines. Which promotes the conduct of numerous in vivo researches to discover an efficient treatment.
{"title":"Anthelmintic Activity of Artemisia monosperma Methanol Extracts against Eisenia fetida in vitro Study","authors":"N. M. Saleh, A.G. Rewaida, A. Afaf, A.A. Sarah, Al-Q. Saleh, M.A. Esam","doi":"10.18805/ijar.bf-1705","DOIUrl":"https://doi.org/10.18805/ijar.bf-1705","url":null,"abstract":"Background: The present investigation was designed to investigate the protective effect of a natural product to control Anthelmintic in earthworm Eisenia fetida. We investigated the effectiveness of dried Artemisia monosperma leaves as an anthelmintic. Methods: Five groups (50, 100, 200 and mg/mL) of the AMLE extract were utilized in this study. Negative and positive controls consisted of distilled water and mebendazole (10 mg/ml). Five worms that were around the same size were placed to each Petri plate along with the test solution (20 mL) of the extract. Artemisia monosperma leaves methanolic extract was prepared and evaluated its ability in vitro as anthelmintic activity against earthworms (Eisenia fetida). The ABTS, FRPA and DPPH techniques were used to calculate the antioxidant activity. Also, the phytochemical investigation of methanolic extract was performed on GC-MS equipment. Result: Fourteen different biomolecules are predicted using GC-MS spectrum. Also, the AMLE had a greater percentage value of ABTS, FRPA and DPPH radical inhibition (94.5±0.03 trolox/gFW, 0.8±0.005 µmol/ gFW and 92.6±0.3%) respectively. Moreover, IC50 of AMLE was obtained at 20.5±0.9 ìg/mL for the A549 and 24.3±0.9 ìg/mL for MCF-7 cell lines. In comparison to AMLE, Mebendazole (10 mg/mL) produced paralysis and earthworm death by 13.91±0.007 and 18.2±0.980 min, respectively, while, AMLE (200 mg/mL) showed 3.860±0.320 and 5.464±0.422 min, respectively. The treated worms’ surface architecture also had noticeable abnormalities, according to the histological study. The result of present study revealed that AMLE leaves can serve as a good natural source of potent antioxidants and anthelminthiasis medicines. Which promotes the conduct of numerous in vivo researches to discover an efficient treatment.\u0000","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"22 12","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138603494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Selvam, N. Murali, A. Thiruvenkadan, G. Ponnudurai, K. T. P. Jawahar, P. Kathiravan
Background: A study was executed to analyze the genetic resistance to gastrointestinal nematodes through the association of single nucleotide polymorphism markers in Toll-like receptor genes with phenotypic indicator traits in Kilakarsal and Vembur sheep breeds. Methods: The phenotypic traits for analyzing the gastrointestinal nematode infection namely FEC, change in PCV and change in body weight were recorded. The SNP markers in TLR3, TLR5, TLR6, TLR9 and TLR10 genes were employed for genotyping. Competitive allele-specific PCR-based endpoint genotyping was used to type the SNPs. The gene and genotype frequencies were estimated by using the PEAS software program. A complete fixed effects model was utilized for analysis of the association of various genotypes at each SNP with phenotypic indicator traits. Result: The global minor allele frequency of different polymorphic SNP loci ranged from 0.06 to 0.48 with a mean of 0.23, signifying their fitness for the association study. The effect of the farm had no significant influence on FEC and change in body weight, however, had a significant effect on change in PCV (P less than 0.05). No significant difference was detected between the sexes with FEC, change in PCV and change in body weight. The TT genotype in the TLR9_1769_CT locus showed the lowest least-squares mean FEC. The remaining 22 SNP loci showed no significant difference (P greater than 0.05) with mean FEC. Association of 23 TLR SNP genotypes with change in PCV and change in body weight revealed no significant effect (P greater than 0.05).
{"title":"Candidate Gene Analysis of Genetic Resistance to Gastrointestinal Nematodes in Sheep Through Association of Single Nucleotide Polymorphism with Phenotypic Traits","authors":"R. Selvam, N. Murali, A. Thiruvenkadan, G. Ponnudurai, K. T. P. Jawahar, P. Kathiravan","doi":"10.18805/ijar.b-5208","DOIUrl":"https://doi.org/10.18805/ijar.b-5208","url":null,"abstract":"Background: A study was executed to analyze the genetic resistance to gastrointestinal nematodes through the association of single nucleotide polymorphism markers in Toll-like receptor genes with phenotypic indicator traits in Kilakarsal and Vembur sheep breeds. Methods: The phenotypic traits for analyzing the gastrointestinal nematode infection namely FEC, change in PCV and change in body weight were recorded. The SNP markers in TLR3, TLR5, TLR6, TLR9 and TLR10 genes were employed for genotyping. Competitive allele-specific PCR-based endpoint genotyping was used to type the SNPs. The gene and genotype frequencies were estimated by using the PEAS software program. A complete fixed effects model was utilized for analysis of the association of various genotypes at each SNP with phenotypic indicator traits. Result: The global minor allele frequency of different polymorphic SNP loci ranged from 0.06 to 0.48 with a mean of 0.23, signifying their fitness for the association study. The effect of the farm had no significant influence on FEC and change in body weight, however, had a significant effect on change in PCV (P less than 0.05). No significant difference was detected between the sexes with FEC, change in PCV and change in body weight. The TT genotype in the TLR9_1769_CT locus showed the lowest least-squares mean FEC. The remaining 22 SNP loci showed no significant difference (P greater than 0.05) with mean FEC. Association of 23 TLR SNP genotypes with change in PCV and change in body weight revealed no significant effect (P greater than 0.05).\u0000","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"38 22","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138602409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Lakshmipriya, D. Sinha, K.M. Singh, Nasim Ahmad, R. Raju, Arnab Roy
Background: Dairy farming has emerged as an important profitable enterprise in India as it is a source of income, especially for small and marginal farmers;the study aims to understand the reasons for increased profit of dairy farmers of South Indian states. Methods: The present study was undertaken in Southern Indian states during 2020-21 to estimate the profit efficiency of milk producers across different herd-size categories. Result: Maximum likelihood estimates (MLE) of specified profit function revealed that overall, prices of green fodder (0.1873), prices of concentrate (0.1072), veterinary service rate (0.0569) and herd size (0.7545), had a positive and significant impact on normalized profits, whereas prices of dry fodder (-0.0277) and labour wages (-0.1652) had a negative and significant impact on normalized profits, respectively. Overall, the mean profit efficiency of dairy farmers was found to be 58.65 per cent indicating 41.35 per cent of profit efficiency was lost due to technical and allocative inefficiencies in milk production. Results from the profit inefficiency model revealed that overall, education, herd size, herd composition and experience in dairy farming had a negative and significant impact on profit inefficiency.
{"title":"Profit Efficiency among Dairy Farmers in Southern India: An Application of Stochastic Frontier Profit Function.","authors":"P. Lakshmipriya, D. Sinha, K.M. Singh, Nasim Ahmad, R. Raju, Arnab Roy","doi":"10.18805/ijar.b-5096","DOIUrl":"https://doi.org/10.18805/ijar.b-5096","url":null,"abstract":"Background: Dairy farming has emerged as an important profitable enterprise in India as it is a source of income, especially for small and marginal farmers;the study aims to understand the reasons for increased profit of dairy farmers of South Indian states. Methods: The present study was undertaken in Southern Indian states during 2020-21 to estimate the profit efficiency of milk producers across different herd-size categories. Result: Maximum likelihood estimates (MLE) of specified profit function revealed that overall, prices of green fodder (0.1873), prices of concentrate (0.1072), veterinary service rate (0.0569) and herd size (0.7545), had a positive and significant impact on normalized profits, whereas prices of dry fodder (-0.0277) and labour wages (-0.1652) had a negative and significant impact on normalized profits, respectively. Overall, the mean profit efficiency of dairy farmers was found to be 58.65 per cent indicating 41.35 per cent of profit efficiency was lost due to technical and allocative inefficiencies in milk production. Results from the profit inefficiency model revealed that overall, education, herd size, herd composition and experience in dairy farming had a negative and significant impact on profit inefficiency.","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"175 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139219096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Cryptosporidium species and Giardia duodenalis are important protozoan parasites due to their wide host range and potential as zoonotic diseases. These parasites, responsible for Cryptosporidiosis and Giardiasis, are significant health concerns and have garnered attention from both the public and veterinary fields. Pets, such as dogs and cats, are susceptible to these infections and play a crucial role in transmitting these protozoans to humans. This study was conducted to determine the prevalence, species, genotypes and zoonotic significance of Cryptosporidium and Giardia in asymptomatic adult stray cats and dogs. Methods: Fecal samples from 75 adult stray animal, 42 dogs and 33 cats, were collected in Diyarbakýr city, southeastern Anatolia, Turkey. Direct immunofluorescence test was used to confirm the presence of G. duoenalis cysts and cryptosporidium spp. oocysts in collected fecal samples. Microscopic analysis was used to count the number of cysts/oocysts per gram as specified by the manufacturer. Molecular confirmation was done with Polymerase chain reaction (PCR) using Giardia SSU-rDNA and Cryptosporidium spp. SSU rRNA gene. The secondary PCR products of all positive samples were sequenced in one directions on an automated sequencer. Nucleotide sequence analysis was performed by BLAST alignment using the National Center for Biotechnology Information database. Result: The prevalence of Cryptosporidium was 7.14% in 42 cats and 9.09% in 33 dogs. The prevalence of G. duodenalis was 23.80% in cats and 54.54% in dogs. The average number of Cryptosporidium spp. oocysts per gram of cat and dog feces was 1866.3 and 1813.3, respectively. The average number of G. duodenalis cysts per gram of cat and dog feces was 1011.6 and 4786, respectively. All Cryptosporidium isolates were determined as C. canis in dogs and C. felis in cats. C. canis and C. felis isolates identified in the study, MT329018.1 and MN696800.1, AF159113.1 and KM977642.1 showed similarity with Genbank number, respectively. Assemblages A, E, C, D were found in dogs and assemblages A and E in cats in the result of the sequence of 130 bp gene fragments obtained from G. duodenalis isolates. This study sequence analysis of G. duodenalis cat isolates identified assemblages A (50%/10) and E (50%/10). In this study, assemblage E was the most common genotype isolated in dogs (38.8%), followed by Assemblage C (27.7%), Assemblage D (16.6%) and Assemblage A (15.5%). Assemblage A, which has zoonotic importance, was detected in cats and dogs, while assemblage E in cats and dogs was reported for the first time in Turkey.
背景：隐孢子虫和十二指肠贾第虫是重要的原生动物寄生虫，因为它们的宿主范围很广，有可能成为人畜共患病。这些寄生虫是隐孢子虫病和贾第鞭毛虫病的罪魁祸首，是重大的健康问题，已引起公众和兽医领域的关注。猫狗等宠物很容易感染这些疾病，并在将这些原生动物传播给人类的过程中扮演着重要角色。本研究旨在确定无症状成年流浪猫和流浪狗中隐孢子虫和贾第鞭毛虫的流行率、种类、基因型和人畜共患意义。研究方法在土耳其安纳托利亚东南部迪亚巴克尔市收集了 75 只成年流浪动物（42 只狗和 33 只猫）的粪便样本。采用直接免疫荧光试验来确认收集的粪便样本中是否存在双孢子虫囊蚴和隐孢子虫卵囊。按照生产商的规定，使用显微镜分析来计算每克样本中的囊肿/卵囊数量。使用聚合酶链反应（PCR）对贾第鞭毛虫 SSU-rDNA 和隐孢子虫 SSU rRNA 基因进行分子确认。所有阳性样本的次级 PCR 产物都在自动测序仪上进行了单向测序。利用美国国家生物技术信息中心数据库进行BLAST比对，对核苷酸序列进行分析。结果42只猫和33只狗的隐孢子虫感染率分别为7.14%和9.09%。猫和狗的十二指肠球菌感染率分别为 23.80%和 54.54%。每克猫和狗粪便中隐孢子虫卵囊的平均数量分别为 1866.3 个和 1813.3 个。每克猫和狗粪便中的十二指肠球孢子虫囊蚴平均数量分别为 1011.6 个和 4786 个。所有隐孢子虫分离物均被确定为犬隐孢子虫和猫隐孢子虫。研究中发现的犬隐孢子虫和猫隐孢子虫分离物 MT329018.1 和 MN696800.1、AF159113.1 和 KM977642.1 分别与 Genbank 编号相似。从十二指肠球菌分离物中获得的 130 bp 基因片段的序列结果显示，在狗中发现了 A、E、C、D 组合，在猫中发现了 A 和 E 组合。本研究对猫十二指肠球菌分离物的序列分析确定了 A（50%/10）和 E（50%/10）群。在本研究中，E 组合是在狗体内分离出的最常见基因型（38.8%），其次是 C 组合（27.7%）、D 组合（16.6%）和 A 组合（15.5%）。在猫和狗中检测到了具有人畜共患病重要性的A基因组，而在猫和狗中检测到E基因组则是土耳其的首次报道。
{"title":"Molecular Characterization and Zoonotic Significance of Cryptosporidium spp. and Giardia duodenalis in Asymptomatic Adult Stray Cats and Dogs in Turkey","authors":"D. Ipek","doi":"10.18805/ijar.bf-1707","DOIUrl":"https://doi.org/10.18805/ijar.bf-1707","url":null,"abstract":"Background: Cryptosporidium species and Giardia duodenalis are important protozoan parasites due to their wide host range and potential as zoonotic diseases. These parasites, responsible for Cryptosporidiosis and Giardiasis, are significant health concerns and have garnered attention from both the public and veterinary fields. Pets, such as dogs and cats, are susceptible to these infections and play a crucial role in transmitting these protozoans to humans. This study was conducted to determine the prevalence, species, genotypes and zoonotic significance of Cryptosporidium and Giardia in asymptomatic adult stray cats and dogs. Methods: Fecal samples from 75 adult stray animal, 42 dogs and 33 cats, were collected in Diyarbakýr city, southeastern Anatolia, Turkey. Direct immunofluorescence test was used to confirm the presence of G. duoenalis cysts and cryptosporidium spp. oocysts in collected fecal samples. Microscopic analysis was used to count the number of cysts/oocysts per gram as specified by the manufacturer. Molecular confirmation was done with Polymerase chain reaction (PCR) using Giardia SSU-rDNA and Cryptosporidium spp. SSU rRNA gene. The secondary PCR products of all positive samples were sequenced in one directions on an automated sequencer. Nucleotide sequence analysis was performed by BLAST alignment using the National Center for Biotechnology Information database. Result: The prevalence of Cryptosporidium was 7.14% in 42 cats and 9.09% in 33 dogs. The prevalence of G. duodenalis was 23.80% in cats and 54.54% in dogs. The average number of Cryptosporidium spp. oocysts per gram of cat and dog feces was 1866.3 and 1813.3, respectively. The average number of G. duodenalis cysts per gram of cat and dog feces was 1011.6 and 4786, respectively. All Cryptosporidium isolates were determined as C. canis in dogs and C. felis in cats. C. canis and C. felis isolates identified in the study, MT329018.1 and MN696800.1, AF159113.1 and KM977642.1 showed similarity with Genbank number, respectively. Assemblages A, E, C, D were found in dogs and assemblages A and E in cats in the result of the sequence of 130 bp gene fragments obtained from G. duodenalis isolates. This study sequence analysis of G. duodenalis cat isolates identified assemblages A (50%/10) and E (50%/10). In this study, assemblage E was the most common genotype isolated in dogs (38.8%), followed by Assemblage C (27.7%), Assemblage D (16.6%) and Assemblage A (15.5%). Assemblage A, which has zoonotic importance, was detected in cats and dogs, while assemblage E in cats and dogs was reported for the first time in Turkey.","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"119 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139221967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amin Al-Ansi, Hathal M. Aldhafer, Jameel Al-Tamimi, M. Murshed
Background: The red flour beetle, Tribolium castaneum (Herbst) (Tenebrionidae, Coleoptera) is a pest of worldwide distribution and can cause destructive damage to stored grains. The physicochemical properties and varied effects against insect pests make plant extracts a potential alternative in the development of pesticides. Methods: In this study, we assayed the oleander leaf extract toxicity effects against T. castaneum adults in the laboratory. We used four concentrations of Oleander methanol leaf extract. Result: Exposure of T. castaneum adult to the oleander leaf extracts produced 100% mortality in the insecticidal bioassay, especially at 40% concentration. The mortality % ranged from 13.3-100% after 48hrs. The mortality percentage of the red flour beetle adults decreased by increasing exposure periods. The mortality % was highly negatively correlated with exposure times (R= -0.97, P =.0001; R= -0.80 and P=.0001) at 40 and 20% concentrations, respectively. The overall results of the current study suggest that the leaf extract of Nerium oleander may possess potential insecticidal properties, which could potentially be employed in pest management. The infrared analysis of the oleander leaf extract showed many bioactive components associated with plant secondary metabolites; some of these identified phytochemical compounds have biological activity. Further, the phenol and flavonoid total were estimated. We conclude that the oleander leaf extract has the potential to be useful in managing stored grain insect pests, particularly T. castaneum, but that it must be handled and applied with extreme caution.
{"title":"Evaluation of the Insecticidal Activity of Nerium oleander L. against the Red Flour Beetle, Tribolium castaneum (Herbst) (Tenebrionidae, Coleoptera)","authors":"Amin Al-Ansi, Hathal M. Aldhafer, Jameel Al-Tamimi, M. Murshed","doi":"10.18805/ijar.bf-1715","DOIUrl":"https://doi.org/10.18805/ijar.bf-1715","url":null,"abstract":"Background: The red flour beetle, Tribolium castaneum (Herbst) (Tenebrionidae, Coleoptera) is a pest of worldwide distribution and can cause destructive damage to stored grains. The physicochemical properties and varied effects against insect pests make plant extracts a potential alternative in the development of pesticides. Methods: In this study, we assayed the oleander leaf extract toxicity effects against T. castaneum adults in the laboratory. We used four concentrations of Oleander methanol leaf extract. Result: Exposure of T. castaneum adult to the oleander leaf extracts produced 100% mortality in the insecticidal bioassay, especially at 40% concentration. The mortality % ranged from 13.3-100% after 48hrs. The mortality percentage of the red flour beetle adults decreased by increasing exposure periods. The mortality % was highly negatively correlated with exposure times (R= -0.97, P =.0001; R= -0.80 and P=.0001) at 40 and 20% concentrations, respectively. The overall results of the current study suggest that the leaf extract of Nerium oleander may possess potential insecticidal properties, which could potentially be employed in pest management. The infrared analysis of the oleander leaf extract showed many bioactive components associated with plant secondary metabolites; some of these identified phytochemical compounds have biological activity. Further, the phenol and flavonoid total were estimated. We conclude that the oleander leaf extract has the potential to be useful in managing stored grain insect pests, particularly T. castaneum, but that it must be handled and applied with extreme caution.","PeriodicalId":13410,"journal":{"name":"Indian Journal of Animal Research","volume":"19 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139218665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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