A. Varghaei, Hengameh Mojdeganlou, M. Rabieepour, Amir Mikaeilvand, A. Abbasi
ILD is a common manifestation of dermatomyositis (DM) with indolent course but a unique variant of DM called clinically amyopathic DM (CADM) presents with rapid progressive interstitial lung disease (RP-ILD) and is called CADM associated RP-ILD. Here, we report a case of a 52 year old woman with CADM associated RP-ILD occurred 2 weeks after recovery from COVID19 infection but unfortunately died from RP-ILD, almost 5 months later. This is the first report of CADM associated RP-ILD triggered by COVID19 in an adult patient
{"title":"First report of an adult patient with Clinical amyopathic dermatomyositis associated rapid progressive interstitial lung disease triggered by COVID19","authors":"A. Varghaei, Hengameh Mojdeganlou, M. Rabieepour, Amir Mikaeilvand, A. Abbasi","doi":"10.34172/ipp.2022.32406","DOIUrl":"https://doi.org/10.34172/ipp.2022.32406","url":null,"abstract":"ILD is a common manifestation of dermatomyositis (DM) with indolent course but a unique variant of DM called clinically amyopathic DM (CADM) presents with rapid progressive interstitial lung disease (RP-ILD) and is called CADM associated RP-ILD. Here, we report a case of a 52 year old woman with CADM associated RP-ILD occurred 2 weeks after recovery from COVID19 infection but unfortunately died from RP-ILD, almost 5 months later. This is the first report of CADM associated RP-ILD triggered by COVID19 in an adult patient","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43809058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Introduction: Recurrent pregnancy loss (RPL) is a common obstetrical complication, where 50% have undetermined etiology. Antiphospholipid syndrome (APL) is an autoimmune disease documented as a cause of RPL. Thrombophilia, particularly platelet abnormalities, were accredited for RPL. Objectives: The purpose of this study is to determine the change in platelets parameters; specifically, platelet count, mean platelet volume (MPV), platelet distribution width PDW, in patients suffering from RPL caused by the anti-phospholipid syndrome. Patients and Methods: Fifty women with a confirmed diagnosis of APL syndrome having a history of RPL were assigned as a study group and fifty women who gave birth without RPL were set as healthy controls were taken from al Yarmouk teaching hospitals in Baghdad. The personal data were taken by direct interview with patients, and blood samples were taken and analyzed for complete blood counts and platelets indices. Results: Both platelets count and PDW scored significant correlation with the number of pregnancy loss; Pearson's correlation coefficient was (0.74,0.59) respectively for both variables. The ROC evaluated platelets count a cutoff value of 230 ,88%,84% respective sensitivity and specificities, AUC =0.89,and P-value<0.001 respectively. As for PDW it had a cutoff value of 13.6 fl/L,88%,80% respective sensitivity and specificities, AUC=0.87,and P-value<0.001. Conclusions: Platelet indicators may aid gynecologists' in low-resource settings in predicting high-risk pregnancies deemed to suffer from RPL .
{"title":"Is There a Change in Platelet Indices Among Pregnant With Anti Phospholipid Syndrome?","authors":"Wassan Nori, Shaima K. Harmoosh, Roaa Hamed","doi":"10.34172/ipp.2022.32410","DOIUrl":"https://doi.org/10.34172/ipp.2022.32410","url":null,"abstract":"Introduction: Recurrent pregnancy loss (RPL) is a common obstetrical complication, where 50% have undetermined etiology. Antiphospholipid syndrome (APL) is an autoimmune disease documented as a cause of RPL. Thrombophilia, particularly platelet abnormalities, were accredited for RPL. Objectives: The purpose of this study is to determine the change in platelets parameters; specifically, platelet count, mean platelet volume (MPV), platelet distribution width PDW, in patients suffering from RPL caused by the anti-phospholipid syndrome. Patients and Methods: Fifty women with a confirmed diagnosis of APL syndrome having a history of RPL were assigned as a study group and fifty women who gave birth without RPL were set as healthy controls were taken from al Yarmouk teaching hospitals in Baghdad. The personal data were taken by direct interview with patients, and blood samples were taken and analyzed for complete blood counts and platelets indices. Results: Both platelets count and PDW scored significant correlation with the number of pregnancy loss; Pearson's correlation coefficient was (0.74,0.59) respectively for both variables. The ROC evaluated platelets count a cutoff value of 230 ,88%,84% respective sensitivity and specificities, AUC =0.89,and P-value<0.001 respectively. As for PDW it had a cutoff value of 13.6 fl/L,88%,80% respective sensitivity and specificities, AUC=0.87,and P-value<0.001. Conclusions: Platelet indicators may aid gynecologists' in low-resource settings in predicting high-risk pregnancies deemed to suffer from RPL .","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43853393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wassan Nori, Roaa Hamed, W. Akram, Maha Chasib Munshid
Coronavirus Disease 2019 is a new and rapidly developing health crisis. Ongoing researches are looking at the prevalence and consequences of COVID-19 in the obstetric community and postnatal period. In COVID 19 era, pregnant mothers are prone to infection with the severe acute respiratory syndrome coronavirus 2, with a higher risk of poor pregnancy outcomes. Therefore, an accurate and early diagnosis is a necessity for this vulnerable group. Screening for asymptomatic carriers is a cornerstone to limit the COVID-19 pandemic. It is vital to evaluate patients’ clinical symptoms and epidemiological history carefully. Although the serological test; Polymerase chain reaction RT-PCR can confirm infection, it cannot determine the degree or severity of the illness. Moreover, it has false-negative results. Imaging tests allow exact diagnosis of lung damage, the severity of the disease, and the classification of patients. Comprehensive analyses of serological and imaging data will assist in the formation of an appropriate clinical diagnosis. This review will discuss the updates and critical points in establishing COVID-19 infection in pregnant women with their pros and cons.
{"title":"The dilemma of COVID-19 Diagnosis in Pregnancy","authors":"Wassan Nori, Roaa Hamed, W. Akram, Maha Chasib Munshid","doi":"10.34172/ipp.2022.32416","DOIUrl":"https://doi.org/10.34172/ipp.2022.32416","url":null,"abstract":"Coronavirus Disease 2019 is a new and rapidly developing health crisis. Ongoing researches are looking at the prevalence and consequences of COVID-19 in the obstetric community and postnatal period. In COVID 19 era, pregnant mothers are prone to infection with the severe acute respiratory syndrome coronavirus 2, with a higher risk of poor pregnancy outcomes. Therefore, an accurate and early diagnosis is a necessity for this vulnerable group. Screening for asymptomatic carriers is a cornerstone to limit the COVID-19 pandemic. It is vital to evaluate patients’ clinical symptoms and epidemiological history carefully. Although the serological test; Polymerase chain reaction RT-PCR can confirm infection, it cannot determine the degree or severity of the illness. Moreover, it has false-negative results. Imaging tests allow exact diagnosis of lung damage, the severity of the disease, and the classification of patients. Comprehensive analyses of serological and imaging data will assist in the formation of an appropriate clinical diagnosis. This review will discuss the updates and critical points in establishing COVID-19 infection in pregnant women with their pros and cons.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43243060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Tabibzadeh, Mohamad hadi Karbalai niya, H. Keyvani, Sajad Karampour, Parastoo Yousefi, Mohammad Hossein Razizadeh, Maryam Esghaei, Abdulhussain Kadhim Jawaziri, ahanbakhsh Khamseh
Background: Coronavirus disease2019 (COVID-19) spread all around the world and leads to many new infection cases and mortality. A better understanding of the SARS-CoV-2 pathogenesis could lead to more efficient therapeutic approaches. The current study aimed to investigate the Toll-like receptors expression profile in peripheral blood mononuclear cells in COVID-19 patients in Iran. Materials and methods: In this cross-sectional study, we evaluate 32 patients. At the admission time based on the disease severity patients were divided into two groups severe and mild COVID-19. A group of 16 normal people was evaluated as the control. Blood samples were collected before any treatment from patients at the admission time. The Blood samples were used for PBMC isolation. RNA extracted from PBMCs and use for the cDNA synthesis. The expression level of TLR-3, 4, and 7 were evaluated by using semi-quantitative real-time PCR. Results: The patient's mean age was 57.12±3.08 years and 15 (47%) were male. The background disorders represent two patients in the severe group with respiratory disorders, 4 patients with cardiovascular disease, and seven patients with diabetes. The TLRs expression levels represent a higher expression of TLR-4 in COVID-19 in comparison with controls for TLR-4 (p=0.00), TLR-3 and 7 also represent up-regulation but there were no statistically significant differences in the relative fold changes (RFC) between groups for all other evaluated TLRs (p>0.05). Conclusion: The study highlights the importance of the TLR-4 in COVID-19 patients in expression level. Further studies for a clear conclusion about TLRs expression levels are recommended.
{"title":"Investigation of the Toll-like receptors expression profile in peripheral blood mononuclear cells in COVID-19 patients in Iran","authors":"A. Tabibzadeh, Mohamad hadi Karbalai niya, H. Keyvani, Sajad Karampour, Parastoo Yousefi, Mohammad Hossein Razizadeh, Maryam Esghaei, Abdulhussain Kadhim Jawaziri, ahanbakhsh Khamseh","doi":"10.34172/ipp.2022.32405","DOIUrl":"https://doi.org/10.34172/ipp.2022.32405","url":null,"abstract":"Background: Coronavirus disease2019 (COVID-19) spread all around the world and leads to many new infection cases and mortality. A better understanding of the SARS-CoV-2 pathogenesis could lead to more efficient therapeutic approaches. The current study aimed to investigate the Toll-like receptors expression profile in peripheral blood mononuclear cells in COVID-19 patients in Iran. Materials and methods: In this cross-sectional study, we evaluate 32 patients. At the admission time based on the disease severity patients were divided into two groups severe and mild COVID-19. A group of 16 normal people was evaluated as the control. Blood samples were collected before any treatment from patients at the admission time. The Blood samples were used for PBMC isolation. RNA extracted from PBMCs and use for the cDNA synthesis. The expression level of TLR-3, 4, and 7 were evaluated by using semi-quantitative real-time PCR. Results: The patient's mean age was 57.12±3.08 years and 15 (47%) were male. The background disorders represent two patients in the severe group with respiratory disorders, 4 patients with cardiovascular disease, and seven patients with diabetes. The TLRs expression levels represent a higher expression of TLR-4 in COVID-19 in comparison with controls for TLR-4 (p=0.00), TLR-3 and 7 also represent up-regulation but there were no statistically significant differences in the relative fold changes (RFC) between groups for all other evaluated TLRs (p>0.05). Conclusion: The study highlights the importance of the TLR-4 in COVID-19 patients in expression level. Further studies for a clear conclusion about TLRs expression levels are recommended.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44034821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ahmadi, N. Mohtasham, A. Shamsizadeh, H. Javaherizadeh, B. Cheraghian, Masumeh Alizadeh
Introduction: Diarrheal disorders remain a major disease burden throughout the world in pediatrics that remains a health problem. Several treatment methods have been proposed to treat diarrhea, however each of them has some problems. Objectives: The aim of this study was the evaluation the effect of oral Saccharomyces boulardii on the duration and severity of colitis. Patients and Methods: This randomized clinical trial study included ninety-four patients with acute colitis were enrolled. The children were divided randomly into case and control groups. The control group received supportive treatment including intravenous or oral fluid therapy (ORS solution), antibiotic treatment with azithromycin suspension, and zinc sulfate syrup daily for five days. In addition to the mentioned treatments, the intervention group received S. boulardii as a probiotic capsule. Then, the duration and severity of diarrhea based on the Vesikari score were evaluated for all the patients for five first days. Results: The mean of Vesikari score after the first day in both groups was similar, while after the third and fifth day in the probiotic group this score was lower than the control group (5.11 ± 1.73 versus 6.13 ± 2.25, P=0.031 and 2.36 ± 1.16 versus 4.28 ±1.19, P<0.001, respectively). Furthermore, the number of diarrhea per day in the probiotic group was significantly lower after the first day in contrast to the control group, which remains significant at the end of the fifth day (2.32 ± 0.51 versus 2.83 ± 1.23, P=0.047). Moreover, we found that the diarrhea duration (P<0.001), fever duration (P=0.029), and duration of hospitalization (P=0.006) were statistically lower in the case group as compared to the control. Conclusion: Saccharomyces boulardii had a beneficial effect as a probiotic agent in reducing the severity and duration of diarrhea, which could be beneficial for the treatment of colitis. Trial Registration: The trial protocol was approved by the Iranian Registry of Clinical Trials (identifier: IRCT20161026030525N2); https://www.irct.ir/trial/24229; ethical code: IR.AJUMS.REC.1395.54.
{"title":"Saccharomyces boulardii plus azithromycin is superior to azithromycin alone in reducing the duration of diarrhea in children with acute colitis: a double-blind randomized placebo-controlled trial","authors":"M. Ahmadi, N. Mohtasham, A. Shamsizadeh, H. Javaherizadeh, B. Cheraghian, Masumeh Alizadeh","doi":"10.34172/ipp.2022.31397","DOIUrl":"https://doi.org/10.34172/ipp.2022.31397","url":null,"abstract":"Introduction: Diarrheal disorders remain a major disease burden throughout the world in pediatrics that remains a health problem. Several treatment methods have been proposed to treat diarrhea, however each of them has some problems. Objectives: The aim of this study was the evaluation the effect of oral Saccharomyces boulardii on the duration and severity of colitis. Patients and Methods: This randomized clinical trial study included ninety-four patients with acute colitis were enrolled. The children were divided randomly into case and control groups. The control group received supportive treatment including intravenous or oral fluid therapy (ORS solution), antibiotic treatment with azithromycin suspension, and zinc sulfate syrup daily for five days. In addition to the mentioned treatments, the intervention group received S. boulardii as a probiotic capsule. Then, the duration and severity of diarrhea based on the Vesikari score were evaluated for all the patients for five first days. Results: The mean of Vesikari score after the first day in both groups was similar, while after the third and fifth day in the probiotic group this score was lower than the control group (5.11 ± 1.73 versus 6.13 ± 2.25, P=0.031 and 2.36 ± 1.16 versus 4.28 ±1.19, P<0.001, respectively). Furthermore, the number of diarrhea per day in the probiotic group was significantly lower after the first day in contrast to the control group, which remains significant at the end of the fifth day (2.32 ± 0.51 versus 2.83 ± 1.23, P=0.047). Moreover, we found that the diarrhea duration (P<0.001), fever duration (P=0.029), and duration of hospitalization (P=0.006) were statistically lower in the case group as compared to the control. Conclusion: Saccharomyces boulardii had a beneficial effect as a probiotic agent in reducing the severity and duration of diarrhea, which could be beneficial for the treatment of colitis. Trial Registration: The trial protocol was approved by the Iranian Registry of Clinical Trials (identifier: IRCT20161026030525N2); https://www.irct.ir/trial/24229; ethical code: IR.AJUMS.REC.1395.54.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":"1 1","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69815146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Bahrami, Soheila Valizadeh, Nasser Aghamohammadzadeh, F. Najafipour, M. Halimi, Reza Javad-Rashid, J. Jalili, Jalil Houshyar
Introduction: Thyroid nodules are considered as a frequent clinical problem. The great majority of thyroid nodules are benign; however malignancy probability is predicted to be 5-10%. The inconsistency between ultrasonography and fine-needle aspiration (FNA) findings is one of important problems in management of thyroid nodules. Objectives: This study was designed to investigate the consistency of ultrasonography, FNA and histopathology findings in thyroid nodules. Patients and Methods: In this descriptive study, 93 patients who were candidate for thyroid surgery were included. Data on ultrasound-guided fine-needle aspiration of the thyroid and histopathologic results before and after surgery were considered. The Cramér’s V and Fisher’s exact tests were conducted for this study. Results: In patients whose ultrasound (US) results were low-suspicious, we observed a significant correlation between FNA and pathology results (Cramér’s V = 0.574, P = 0.037). However, relationship between FNA and pathology was not accessible among patients whose ultrasonography results were intermediate suspicious or high-suspicious due to insufficient number of samples. Conclusion: In our study, a significant correlation was observed between FNA and pathology results in low-suspicious nodules.
简介:甲状腺结节被认为是一个常见的临床问题。绝大多数甲状腺结节是良性的;然而，恶性概率预测为5-10%。超声检查与细针穿刺(FNA)检查结果的不一致是甲状腺结节治疗的重要问题之一。目的:探讨甲状腺结节的超声、FNA及病理检查结果的一致性。患者和方法:在这项描述性研究中，纳入了93例甲状腺手术候选者。考虑超声引导下甲状腺细针穿刺的数据和手术前后的组织病理学结果。cramsamr V和Fisher的精确测试是为了这项研究而进行的。结果:在超声(US)结果为低可疑的患者中，我们观察到FNA与病理结果有显著相关性(cramsamrs’s V = 0.574, P = 0.037)。但在超声检查结果为中度可疑或高度可疑的患者中，由于样本数量不足，FNA与病理的关系尚不明确。结论:在我们的研究中，FNA与低可疑结节的病理结果有显著的相关性。
{"title":"Consistency analysis of ultrasound-guided fine-needle aspiration and histopathology results in thyroid nodules","authors":"A. Bahrami, Soheila Valizadeh, Nasser Aghamohammadzadeh, F. Najafipour, M. Halimi, Reza Javad-Rashid, J. Jalili, Jalil Houshyar","doi":"10.34172/ipp.2022.31350","DOIUrl":"https://doi.org/10.34172/ipp.2022.31350","url":null,"abstract":"Introduction: Thyroid nodules are considered as a frequent clinical problem. The great majority of thyroid nodules are benign; however malignancy probability is predicted to be 5-10%. The inconsistency between ultrasonography and fine-needle aspiration (FNA) findings is one of important problems in management of thyroid nodules. Objectives: This study was designed to investigate the consistency of ultrasonography, FNA and histopathology findings in thyroid nodules. Patients and Methods: In this descriptive study, 93 patients who were candidate for thyroid surgery were included. Data on ultrasound-guided fine-needle aspiration of the thyroid and histopathologic results before and after surgery were considered. The Cramér’s V and Fisher’s exact tests were conducted for this study. Results: In patients whose ultrasound (US) results were low-suspicious, we observed a significant correlation between FNA and pathology results (Cramér’s V = 0.574, P = 0.037). However, relationship between FNA and pathology was not accessible among patients whose ultrasonography results were intermediate suspicious or high-suspicious due to insufficient number of samples. Conclusion: In our study, a significant correlation was observed between FNA and pathology results in low-suspicious nodules.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43870931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Valibeik, Narges Tavakoli, H. Amiri, Rouhollah Heydari, Leila Hasanvand, R. Rostami, Niloufar Tavakoli Dastjerd, H. Ahmadvand
Background: Prangos uloptera is a medicinal plant from the Apiaceae family which is native to Iran. Objectives: In the current study, phytochemical constituents of the essential oils, antibacterial and antioxidant activities of methanolic extract of the aerial parts of P. uloptera were investigated. Materials and Methods: To determine the main ingredients of the essential oils and to investigate the antioxidant and antibacterial activities of this plant, the GC-MS (gas chromatography - mass spectrometry) analysis, DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and microdilution method were used. Results: The GC–MS analysis showed that the leading constituents of the essential oils were butyl octanoate (24.88%), 9-Octadecenoic acid (Z) (14.19%) and alpha-pinene (19.28%) in the flowers, fruit and leaves, respectively. The greatest amount of phenolic compound was observed in methanolic extract of the leaves (0.5 ± 0.11 mgGAEs/g). Likewise, the greatest level of flavonoids (0.074 ± 12.4 mg QEs/g) was identified in the leaves. Furthermore, DPPH assay showed the most radical scavenging activity (IC50 201.7 ± 20.39 μg/mL) in the methanolic extract of the leaves. The highest total antioxidant capacity was observed in the fruit (0.004 ± 0.0005 g AAE/ml). The antibacterial activity of the plant extracts against the pathogenic fungal and bacterial species was investigated by broth microdilution method. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) amounts were in the ranges of 312 to 10000 and 1000 to 10000 mg/ml, respectively. Conclusion: The results of this research demonstrated that P. uloptera was a potent source of useful bioactive compounds, making it a promising candidate for further studies.
{"title":"Composition of the essential oils, antioxidant and antibacterial activities of the methanolic extract of Prangos uloptera","authors":"Ali Valibeik, Narges Tavakoli, H. Amiri, Rouhollah Heydari, Leila Hasanvand, R. Rostami, Niloufar Tavakoli Dastjerd, H. Ahmadvand","doi":"10.34172/ipp.2022.29278","DOIUrl":"https://doi.org/10.34172/ipp.2022.29278","url":null,"abstract":"Background: Prangos uloptera is a medicinal plant from the Apiaceae family which is native to Iran. Objectives: In the current study, phytochemical constituents of the essential oils, antibacterial and antioxidant activities of methanolic extract of the aerial parts of P. uloptera were investigated. Materials and Methods: To determine the main ingredients of the essential oils and to investigate the antioxidant and antibacterial activities of this plant, the GC-MS (gas chromatography - mass spectrometry) analysis, DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and microdilution method were used. Results: The GC–MS analysis showed that the leading constituents of the essential oils were butyl octanoate (24.88%), 9-Octadecenoic acid (Z) (14.19%) and alpha-pinene (19.28%) in the flowers, fruit and leaves, respectively. The greatest amount of phenolic compound was observed in methanolic extract of the leaves (0.5 ± 0.11 mgGAEs/g). Likewise, the greatest level of flavonoids (0.074 ± 12.4 mg QEs/g) was identified in the leaves. Furthermore, DPPH assay showed the most radical scavenging activity (IC50 201.7 ± 20.39 μg/mL) in the methanolic extract of the leaves. The highest total antioxidant capacity was observed in the fruit (0.004 ± 0.0005 g AAE/ml). The antibacterial activity of the plant extracts against the pathogenic fungal and bacterial species was investigated by broth microdilution method. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) amounts were in the ranges of 312 to 10000 and 1000 to 10000 mg/ml, respectively. Conclusion: The results of this research demonstrated that P. uloptera was a potent source of useful bioactive compounds, making it a promising candidate for further studies.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43430821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Bahrami, F. Ghorbani, Nasser Aghamohammadzadeh, Farzad Najafipur, Vahieh Sadra
Introduction: Autoimmune thyroid diseases are common findings in women of childbearing age that could affect fertility rate, pregnancy outcome and complications after pregnancy. These diseases also correlated with other autoimmune diseases. Objectives: In this study, we aim to evaluate the autoimmune thyroid disease in women aged 35 to 45 years based on Azar cohort study data. Patients and Methods: In this cross-sectional analytical study, 507 women 35-45 years old without previous thyroid disease from Azar cohort study were evaluated. Demographic findings, data related to previous pregnancies, abortion, miscarriage, infertility and gestational diabetes were recorded for all patients. Blood samples were taken from all patients to measure TSH and anti-thyroid peroxidase (anti-TPO) levels. Anti-TPO levels ≥40 IU/mL were considered positive. Results: In the studied women, miscarriage occurred in 5.3%, abortion in 27.6% and infertility in 7.3%. Gestational diabetes was reported in 3.9%. The positive anti-TPO prevalence was 21.5%. Patients with positive anti-TPO compared to those with negative anti-TPO had significantly higher cases of hypothyroidism (P<0.001). There was no difference between patients with and without positive anti-TPO regarding miscarriage (5% versus 6.4%), abortion (27.4% versus 28.4%), infertility (7.5% versus 6.4%) and gestational diabetes (7.9% versus 3.6%) (P>0.05). There was no correlation between age and anti-TPO levels. Conclusion: The prevalence of thyroid antibodies in our study population is higher than previous studies. Anti-TPO positivity has no significant effect on pregnancy outcome in this population. Further studies are necessary to define the exact effect of anti-TPO on pregnancy outcomes.
{"title":"Autoimmune thyroid disease in women with ages between 35 to 45 years based on Azar cohort data","authors":"A. Bahrami, F. Ghorbani, Nasser Aghamohammadzadeh, Farzad Najafipur, Vahieh Sadra","doi":"10.34172/ipp.2022.25265","DOIUrl":"https://doi.org/10.34172/ipp.2022.25265","url":null,"abstract":"Introduction: Autoimmune thyroid diseases are common findings in women of childbearing age that could affect fertility rate, pregnancy outcome and complications after pregnancy. These diseases also correlated with other autoimmune diseases. Objectives: In this study, we aim to evaluate the autoimmune thyroid disease in women aged 35 to 45 years based on Azar cohort study data. Patients and Methods: In this cross-sectional analytical study, 507 women 35-45 years old without previous thyroid disease from Azar cohort study were evaluated. Demographic findings, data related to previous pregnancies, abortion, miscarriage, infertility and gestational diabetes were recorded for all patients. Blood samples were taken from all patients to measure TSH and anti-thyroid peroxidase (anti-TPO) levels. Anti-TPO levels ≥40 IU/mL were considered positive. Results: In the studied women, miscarriage occurred in 5.3%, abortion in 27.6% and infertility in 7.3%. Gestational diabetes was reported in 3.9%. The positive anti-TPO prevalence was 21.5%. Patients with positive anti-TPO compared to those with negative anti-TPO had significantly higher cases of hypothyroidism (P<0.001). There was no difference between patients with and without positive anti-TPO regarding miscarriage (5% versus 6.4%), abortion (27.4% versus 28.4%), infertility (7.5% versus 6.4%) and gestational diabetes (7.9% versus 3.6%) (P>0.05). There was no correlation between age and anti-TPO levels. Conclusion: The prevalence of thyroid antibodies in our study population is higher than previous studies. Anti-TPO positivity has no significant effect on pregnancy outcome in this population. Further studies are necessary to define the exact effect of anti-TPO on pregnancy outcomes.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45724329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus originates from Wuhan, China; it has spread around the world. According to studies, the results of the SARS-CoV-2 test have been reported positive for some pregnant women. However, not much is known about the effect of this virus on pregnancy and the outcome of baby. The aim of this review study was to evaluate the molecular and diagnostic approach in evaluating the effect of coronavirus disease 2019 (COVID-19) on pregnancy loss. The entry of COVID-19 virus into the pregnant mother’s body through various channels, such as the angiotensin-converting enzyme receptor (ACE2) affects immune and coagulation systems and hormone levels. These changes include D-Dimer, platelets and antithrombin III (AT-III) raises and protein C (PC) decrement and also elevated levels of pro-inflammatory cytokines, including IL-6, followed by disruption of various signaling pathways such as JAK / STAT and PI3K. Additionally, decreased expression of cyclooxygenase 1 (COX1) and prostaglandin E2 (PGE2) and hormones such as progesterone were observed. These changes ultimately lead to serious pregnancy risks, including miscarriage. Therefore, identifying pathways by which COVID-19 impairs immune and coagulation systems of pregnant women can be a way to design abortion preventive strategies.
{"title":"The evaluation of COVID-19 effect on pregnancy loss; a molecular and diagnostic approach","authors":"Narjes Afrooz, Seyedeh Kosar Mahmoudi, Mina Yazdizadeh, Zahra Jahanshahiafshar, Neda Sabernia, Marzieh Rohaninasab","doi":"10.34172/ipp.2022.30332","DOIUrl":"https://doi.org/10.34172/ipp.2022.30332","url":null,"abstract":"The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus originates from Wuhan, China; it has spread around the world. According to studies, the results of the SARS-CoV-2 test have been reported positive for some pregnant women. However, not much is known about the effect of this virus on pregnancy and the outcome of baby. The aim of this review study was to evaluate the molecular and diagnostic approach in evaluating the effect of coronavirus disease 2019 (COVID-19) on pregnancy loss. The entry of COVID-19 virus into the pregnant mother’s body through various channels, such as the angiotensin-converting enzyme receptor (ACE2) affects immune and coagulation systems and hormone levels. These changes include D-Dimer, platelets and antithrombin III (AT-III) raises and protein C (PC) decrement and also elevated levels of pro-inflammatory cytokines, including IL-6, followed by disruption of various signaling pathways such as JAK / STAT and PI3K. Additionally, decreased expression of cyclooxygenase 1 (COX1) and prostaglandin E2 (PGE2) and hormones such as progesterone were observed. These changes ultimately lead to serious pregnancy risks, including miscarriage. Therefore, identifying pathways by which COVID-19 impairs immune and coagulation systems of pregnant women can be a way to design abortion preventive strategies.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42978727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saeid Hosseininia, Susan Mohammadi-Kebar, F. Pourfarzi, M. Bahadoram, Masoud Aslani
Introduction: Chronic obstructive pulmonary disease (COPD) is a disease that is systematically characterized by an abnormal inflammatory response affecting the airways, interstitium and vascular bed through reactions to gas and particles, especially cigarette smoking. Recent studies have shown an association between Helicobacter pylori infection and various inflammatory diseases. H. pylori is a gram-negative, microbial bacterium that can be resistant to acidic stomach conditions and can interfere with gastric urease production. In this study, we examined the relationship between H. pylori infection in patients with COPD and the prevalence of H. pylori infection. Objectives: Determining the association between H. pylori infection and COPD. Patients and Methods: This case-control study is based on the Persian cohort study of patients who were referred to the digestive disease research center after being identified in the pulmonary clinic for H. pylori fecal antigen. Information on demographic variables and other related variables were obtained. Finally, the collected information was entered into SPSS software version 24 and the results were displayed descriptively using distribution and frequency tables and graphs and analytical statistics were analyzed using t test and logistic regression. Results: Out of 250 patients, 134 (53.6%) tested positive for H. pylori and 116 (46.4%) tested negative. Out of a total of 250 non-infected people; 106 patients (42.4%) were positive and 144 patients (57.6%) were negative. The two groups were statistically significantly different based on the chi-square test (P = 0.012). Conclusion: Our study showed a direct and significant relationship between H. pylori and COPD, which can be due to the effect of bacteria on lung growth in early life and also the development of systemic inflammation throughout life.
{"title":"Association of Helicobacter pylori infection with chronic obstructive pulmonary disease","authors":"Saeid Hosseininia, Susan Mohammadi-Kebar, F. Pourfarzi, M. Bahadoram, Masoud Aslani","doi":"10.34172/ipp.2022.32412","DOIUrl":"https://doi.org/10.34172/ipp.2022.32412","url":null,"abstract":"Introduction: Chronic obstructive pulmonary disease (COPD) is a disease that is systematically characterized by an abnormal inflammatory response affecting the airways, interstitium and vascular bed through reactions to gas and particles, especially cigarette smoking. Recent studies have shown an association between Helicobacter pylori infection and various inflammatory diseases. H. pylori is a gram-negative, microbial bacterium that can be resistant to acidic stomach conditions and can interfere with gastric urease production. In this study, we examined the relationship between H. pylori infection in patients with COPD and the prevalence of H. pylori infection. Objectives: Determining the association between H. pylori infection and COPD. Patients and Methods: This case-control study is based on the Persian cohort study of patients who were referred to the digestive disease research center after being identified in the pulmonary clinic for H. pylori fecal antigen. Information on demographic variables and other related variables were obtained. Finally, the collected information was entered into SPSS software version 24 and the results were displayed descriptively using distribution and frequency tables and graphs and analytical statistics were analyzed using t test and logistic regression. Results: Out of 250 patients, 134 (53.6%) tested positive for H. pylori and 116 (46.4%) tested negative. Out of a total of 250 non-infected people; 106 patients (42.4%) were positive and 144 patients (57.6%) were negative. The two groups were statistically significantly different based on the chi-square test (P = 0.012). Conclusion: Our study showed a direct and significant relationship between H. pylori and COPD, which can be due to the effect of bacteria on lung growth in early life and also the development of systemic inflammation throughout life.","PeriodicalId":13454,"journal":{"name":"Immunopathologia Persa","volume":" ","pages":""},"PeriodicalIF":0.8,"publicationDate":"2022-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45315597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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