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Effects of sprouting on the antioxidant potentials of garlic (Allium sativum L.) And onions (Allium cepa L.) 发芽对大蒜(Allium sativum L.)和洋葱(Allium cepa L.)抗氧化潜力的影响
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.7
J.O. Ebhomienlen, M.A Azeke, B.D. Kehinde, D.C. Nwikwe, T.E. Akintelu, B.P. Akwu
No Abstract
无摘要
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引用次数: 0
Preparation of styrene-based imprinted polymer for the adsorption of hazardous bromocresol green dye: Equilibrium, kinetics and thermodynamics study 制备用于吸附有害溴甲酚绿染料的苯乙烯基印迹聚合物:平衡、动力学和热力学研究
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.4
K. Awokoya, V. O. Oninla, O. A. Ogunkunle, B.A. Oyebode, O.J. Owoade, O.O. Obitusin, D.T. Ipadeola
In this study, styrene was used as a functional monomer for the production of molecularly imprinted polymer (MIP) via free radical  polymerization method, using bromocresol green dye (BCG) as template. A non-imprinted polymer (NIP), that excluded the template,  was also synthesized. Both polymers were applied for the removal of BCG from aqueous medium. The synthesized polymers were  characterized with Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray powder diffraction spectroscopic  techniques. The effect of operating variables, such as pH, contact time, initial dye concentration and process temperature, on the efficiencies of the polymers in removing the dye were evaluated. Equilibrium time of BCG adsorption onto the  MIP was reached within  40 min, with adsorption capacity of 49.68 mg g-1 . The adsorption process followed the pseudo-second-order kinetic and Freundlich  isotherm models, while chemisorption mechanism was predicted. The adsorption process was spontaneous and exothermic in nature,  with DH values of -140.03 and -25.01 kJ/mol-1 recorded for BCG removal by MIP and NIP, respectively. The styrene-based MIP showed  good mechanical stability, and retained up to about 99% of its adsorption capacity after six sequential cycles of   regeneration.  Comparatively, the MIP (99.81mg g-1 ) performed considerably better than its NIP (59.00 mg g-1 ) analogue in the removal of BCG from  aqueous medium, thus affirming the potentials of the molecular imprinting technique in the production of efficient adsorbents for  adsorption of toxic dyes from industrial effluents. 
本研究以苯乙烯为功能单体,以溴甲酚绿染料(BCG)为模板,通过自由基聚合法生产分子印迹聚合物(MIP)。此外,还合成了不含模板的非压印聚合物(NIP)。这两种聚合物都被用于去除水介质中的 BCG。利用傅立叶变换红外光谱、扫描电子显微镜和 X 射线粉末衍射光谱技术对合成的聚合物进行了表征。评估了 pH 值、接触时间、初始染料浓度和工艺温度等操作变量对聚合物去除染料效率的影响。卡介苗在 MIP 上的吸附平衡时间为 40 分钟,吸附容量为 49.68 mg g-1。吸附过程遵循伪二阶动力学模型和 Freundlich 等温线模型,同时预测了化学吸附机理。吸附过程为自发放热,MIP 和 NIP 去除卡介苗的 DH 值分别为 -140.03 和 -25.01 kJ/mol-1。苯乙烯基 MIP 具有良好的机械稳定性,在连续再生六次后,其吸附能力仍保持在 99% 左右。 相比之下,MIP(99.81 毫克 g-1)在从水介质中去除卡介苗方面的性能大大优于其类似物 NIP(59.00 毫克 g-1),从而肯定了分子印迹技术在生产高效吸附剂以吸附工业废水中的有毒染料方面的潜力。
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引用次数: 0
Structural characterization and evaluation of mycogenic zinc oxide nanoparticles from the cell-free culture-extract of Aspergillus niger 黑曲霉无细胞培养提取物中的霉源性纳米氧化锌的结构特征和评估
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.9
A.K. Ajijolakewu, M.O. Kazeem, G.M. Dovia, I.A. Adebayo, N.T. Ajide-Bamigboye
Advances in the biological synthesis of nanoparticles have attracted decisive research attention in recent years. This is due to their eco- friendliness, nontoxicity and large spectra of applications. In this work, the structural characteristics and the purity of biogenic zinc oxide  (ZnO) nanoparticles were assessed. Zinc oxide (ZnO) nanoparticles were synthesized extracellularly using the culture filtrate of  Aspergillus niger, in the presence of zinc acetate dihydrate, as a precursor. The structural characteristics and purity of the nanoparticles  were examined using standard characterization methods viz UV-visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier-transmission infrared (FT-IR) spectroscopy and X-ray diffraction (XRD). Results revealed a peak at  311nm and whitish and spherical particles with particulate sizes between 30 and 40 nm for the UV- -1 visible spectroscopy and SEM  respectively. On the FT-IR scale, absorption peaked at 548 cm in the spectra region known for the functional groups of ZnO nanoparticles;  while the average crystalline size was 21 nm based on XRD analysis. Findings in this study revealed that the ecofriendly  biogenic nanoparticles synthesized by common fungi, such as Aspergillus niger, possess desirable qualities comparable to those from  non-ecofriendly and costly chemical processes, which are currently employed for an array of applications.  
近年来,纳米粒子生物合成技术的进步引起了研究人员的极大关注。这得益于其生态友好性、无毒性和广泛的应用范围。本研究评估了生物纳米氧化锌(ZnO)的结构特征和纯度。以黑曲霉的培养滤液为前驱体,在二水醋酸锌的存在下,在细胞外合成了氧化锌(ZnO)纳米粒子。使用标准表征方法,即紫外可见光谱法、透射电子显微镜法(TEM)、扫描电子显微镜法(SEM)、傅立叶透射红外光谱法(FT-IR)和 X 射线衍射法(XRD),对纳米粒子的结构特征和纯度进行了检测。结果显示,紫外-1 可见光谱和扫描电子显微镜分别显示出 311 纳米波长处的峰值和粒径介于 30 纳米和 40 纳米之间的白色球形颗粒。在傅立叶变换红外光谱中,吸收峰值出现在 548 厘米处,该光谱区域是氧化锌纳米粒子的已知官能团;而根据 XRD 分析,平均结晶尺寸为 21 纳米。这项研究的结果表明,由黑曲霉等普通真菌合成的生态友好型生物纳米粒子具有与非生态友好型和昂贵的化学工艺合成的纳米粒子相媲美的理想品质。
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引用次数: 0
Physical quality and antibiotic susceptibility profile of Escherichia coli isolates from roof-harvested rainwater: A microcosm study 从屋顶收集的雨水中分离出的大肠埃希菌的物理质量和抗生素敏感性概况:微观世界研究
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.5
M.A. Titilawo, C.G. Josiah, O.N. Simeon, C.M. Ali, C.S. Ugwuocha, G.O. Ojediran, O. Y. Titilawo, J. Olaitan
Owing to the inadequate supply of potable water in rural settlements in developing countries, roof-harvested rainwater (RHRW) has been  favoured as an alternative source of water supply. The present study investigated the physical characteristics and microbial quality  of 10 RHRW samples collected from storage tanks in Ikwo community of Ebonyi State, Nigeria. Triplicate water sampling was performed  over a 3-month sampling regime. Onsite determination of odour, colour, pH and temperature, and isolation and enumeration of  Escherichia coli were done using standard procedures. The identity of the isolates was confirmed using molecular techniques, and the  susceptibility of the isolates to 11 antibiotics was determined following the Kirby-Bauer disc diffusion assay. Multiple antibiotic resistance  phenotypes and indices (MARPs and MARI) were equally determined. Our findings revealed that the RHRWs had unobjectionable odour  and were colourless. Across the 3-month o sampling, the pH ranged from 6.9 to 7.8 while the temperature was between 24 °C and 29 C.  While E. coli was absent in samples E (September), H (August and October) and J (August, September, and October), the highest E. coli  count in August, September, and October was 37, 32, and 38 cfu/100 mL, respectively. The antibiotic susceptibility test on 40 E. coli  isolates showed high resistance to ampicillin (100%), aztreonam (98%), cefotetan (97%), nitrofurantoin (90%), imipenem (83%),  streptomycin (72%), and ertapenem (69%). Interestingly, the  ighest MARP, 11 (AK/S/ETP/IMI/OFX/NOR/AMP/CTT/ATM/F/TE) (sample B),  and the least, 4 (IMI/AMP/CTT/ATM) were recorded in samples B and D, respectively in September. MARI was from 0.42 to 1.00. The  physical parameters conform with WHO standards, on the other hand, detection of E. coli poses risks of water-borne illness(es) and  transmission of antimicrobial resistance. Hence stringent measures including proper treatment, sanitation and hygiene are advocated to  safeguard the health of dwellers who depend on this water source for daily life activities. 
由于发展中国家农村地区饮用水供应不足,屋顶收集的雨水(RHRW)作为一种替代水源受到青睐。本研究调查了从尼日利亚埃邦伊州 Ikwo 社区储水箱中收集的 10 份屋顶收集雨水样本的物理特征和微生物质量。水样一式三份,采样期为 3 个月。采用标准程序对气味、颜色、pH 值和温度进行了现场测定,并对大肠杆菌进行了分离和计数。采用分子技术确认了分离物的身份,并通过柯比鲍尔盘扩散试验确定了分离物对 11 种抗生素的敏感性。同时还测定了多种抗生素耐药性表型和指数(MARPs 和 MARI)。我们的研究结果表明,RHRW 无色无味。虽然 E(9 月)、H(8 月和 10 月)和 J(8 月、9 月和 10 月)样本中没有大肠杆菌,但 8 月、9 月和 10 月样本中的大肠杆菌最高含量分别为 37、32 和 38 cfu/100 mL。对 40 个大肠杆菌分离株进行的抗生素药敏试验显示,它们对氨苄西林(100%)、阿曲南(98%)、头孢替坦(97%)、硝基呋喃妥因(90%)、亚胺培南(83%)、链霉素(72%)和厄他培南(69%)具有高度耐药性。有趣的是,9 月份样本 B 和样本 D 的 MARP 最高,分别为 11(AK/S/ETP/IMI/OFX/NOR/AMP/CTT/ATM/F/TE)(样本 B),最低,分别为 4(IMI/AMP/CTT/ATM)。MARI 为 0.42 至 1.00。一方面,物理参数符合世界卫生组织的标准,另一方面,检测到大肠杆菌会带来水传播疾病和抗菌药耐药性传播的风险。因此,建议采取包括适当处理、环境卫生和个人卫生在内的严格措施,以保障依赖该水源进行日常生活活动的居民的健康。
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引用次数: 0
Advancing supercapacitor performance: a comprehensive review of electrochemical conversion of coconut shells into activated carbon nanofibers 提升超级电容器性能:椰壳电化学转化为纳米活性炭纤维的综合评述
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.15
I.I. Ajibade, B. Garba, P. Suriati
This assessment provides a comprehensive evaluation of the limitations associated with the application of supercapacitors, along with  the imperative to enhance their functionality. Following this, the advantages of Electrochemical Double Layer Capacitors (EDLC) are  discussed in comparison to other types utilized in supercapacitor contexts. The transformation of coconut shells into carbon nanofibers is  extensively investigated through various methodologies, highlighting both their benefits and limitations. It becomes evident that the  current utilization of coconut shells has not yet achieved optimal sustainability or viability for energy storage purposes. Nevertheless,  coconut shells offer a widely available and sustainable resource that can be converted into Activated Carbon nanofibers for energy  storage applications. Diverse techniques have been employed to produce these ACB nanofibers, each targeting specific objectives  including improved energy density, adaptable diameter, reduced energy consumption, and faster charging times. Despite these accomplishments, it is evident that numerous significant properties of carbon nanofibers derived from coconut shells remain unexplored,  leading to substantial knowledge gaps that must be addressed for each technique. Therefore, further research is warranted  to advance the comprehension of key parameters associated with various methods, ultimately facilitating the development of highly  desirable carbon nanofibers sourced from coconut shells and catering to the requirements of sustainable energy storage applications. 
该评估全面评估了超级电容器应用的局限性,以及增强其功能的必要性。随后,讨论了电化学双层电容器(EDLC)与超级电容器中使用的其他类型相比的优势。通过各种方法对椰子壳转化为碳纳米纤维进行了广泛研究,强调了其优点和局限性。显而易见的是,目前对椰子壳的利用还没有达到最佳的可持续性或能量存储的可行性。尽管如此,椰子壳仍是一种可广泛利用的可持续资源,可转化为活性炭纳米纤维用于储能应用。人们采用了多种技术来生产这些活性碳纳米纤维,每种技术都针对特定的目标,包括提高能量密度、适应直径、降低能耗和加快充电时间。尽管取得了这些成就,但从椰子壳中提取的碳纳米纤维的许多重要特性显然仍未得到探索,导致每种技术都存在大量必须解决的知识差距。因此,有必要开展进一步研究,以加深对各种方法相关关键参数的理解,最终促进开发出非常理想的椰壳纳米碳纤维,满足可持续能源存储应用的要求。
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引用次数: 0
in vitro β-hematin inhibitory activity and antioxidant properties of four selected medicinal plants used in the management of malaria in southwestern Nigeria 尼日利亚西南部用于治疗疟疾的四种精选药用植物的体外 β-血蛋白抑制活性和抗氧化特性
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.13
G.O. Omotosho, G.E. Ogundepo, J.I. Olawuni, N.O. Omisore, E. Obuotor, B.A. Akinpelu, O. O. Babalola
No Abstract
无摘要
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引用次数: 0
Kinetics of antibacterial activities of cellulose nanocrystals and their silver-zinc oxide nanocomposites: Application as potential disinfectants 纤维素纳米晶体及其银氧化锌纳米复合材料的抗菌活性动力学:作为潜在消毒剂的应用
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.3
F.O. Agunbiade, E.E. Allen, G.O. Oyetibo
Cellulose nanocrystals (CNCs) were produced from corncob and used as reducing agent in the formation of ZnO/CNCs, Ag/CNCs, and  ZnO-Ag/CNCs composites and applied as antibacterial agents for both grampositive and gram-negative bacteria and the kinetics of  microbial growth inhibition studied. The CNCs and composites were characterized by UV-visible and Fourier transform-infrared (FTIR)  spectroscopy. The characterization results revealed that the functional groups of CNCs were affected by their interactions with Ag+  and Zn2+ ions. The composites at 100 mg/mL displayed activities against Gram-positive bacteria - Bacillus subtilis, Staphylococcus aureus,  Staphylococcus epidermis, Bacillus coagulans and Enterococcus faecalis and Gram-negative bacteria - Escherichia coli, Klebsiella pneumonia,  Salmonella typhimurium, Pseudomonas aeruginosa, Enterococcus faecium and Acinetobacter baumanii) bacteria with inhibitory zones ranged  between 9-46 mm. The kinetics of inhibition showed that the composites treated water had 4 to 28 times lower rate constants compared  to the untreated water. This implied that the composites inhibit growth rates and have the potential of disinfecting for between 4 to 28 h  depending on the bacteria. The materials showed better growth inhibition rate on gram-positive bacteria than on gram-negative. These  composites may have potential applications as disinfectants in personal care products and serve as more eco-friendly alternative  disinfectants to chlorophenols. 
以玉米芯为原料制备了纤维素纳米晶体(CNCs),并将其作为还原剂用于形成 ZnO/CNCs、Ag/CNCs 和 ZnO-Ag/CNCs 复合材料,将其用作革兰氏阳性菌和革兰氏阴性菌的抗菌剂,并研究了抑制微生物生长的动力学。通过紫外可见光谱和傅立叶变换红外光谱对 CNCs 和复合材料进行了表征。表征结果表明,氯化萘的官能团受其与 Ag+ 和 Zn2+ 离子相互作用的影响。100 mg/mL 的复合材料对革兰氏阳性菌--枯草芽孢杆菌、金黄色葡萄球菌、表皮葡萄球菌、凝结芽孢杆菌和粪肠球菌,以及革兰氏阴性菌--大肠埃希菌、肺炎克雷伯菌、沙门氏菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌、痢疾杆菌等具有活性、肺炎克雷伯菌、伤寒沙门氏菌、铜绿假单胞菌、粪肠球菌和鲍曼不动杆菌)细菌,抑菌区在 9-46 毫米之间。抑菌动力学表明,与未经处理的水相比,经过复合材料处理的水的速率常数低 4 到 28 倍。这意味着复合材料可以抑制细菌的生长速度,并具有 4 至 28 小时的消毒潜力,具体取决于细菌的种类。与革兰氏阴性菌相比,这些材料对革兰氏阳性菌的生长抑制率更高。这些复合材料有可能用作个人护理产品的消毒剂,并可作为氯酚消毒剂的更环保的替代品。
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引用次数: 0
Response surface optimization of lipase production by Pseudomonas sp. on a low-cost shea-nut cake using solid state fermentation 利用固态发酵法优化假单胞菌在低成本乳木果饼上生产脂肪酶的响应面
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.1
M.O. Kazeem, M.A. Mayaki, D. Musa
Shea nut cake (SNC) is the solid residue produced after the shea butter extraction process. The SNC could provide a low-cost substrate for  bacterial lipase production. Greater yield are among the benefits Solid State Fermentation (SSF). However, there have been less  instances of lipase production by bacteria than fungi in SSF. The current study aimed at improving bacterial lipase production on SNC in  SSF using Response Surface Methodology. Lipase-producing bacteria (LPB) were isolated from the shea butter mill effluent pond and screened for lipase production on a tributyrin agar plate. The influence of four independent parameters (temperature, pH, moisture  content and biosurfactant) on lipase production was studied using Box Behnken Design (BBD) of RSM. Results showed that isolate AO  (Pseudomonas sp.) displayed the highest zone of hydrolysis 2 (53 mm). A quadratic regression with R = 0.931 showed that the model is  best fitting, and predicted the optimum conditions of significant (P>0.05) growth parameters, including temperature at 40°C, moisture content at 50%, and biosurfactant at 2 mL, which produced 408.25 U/g of experimental lipase. The findings indicate that the model was useful for predicting optimal growth conditions for incresing lipase production in SSF and the low-cost agro- industrial SNC could be a promising substrate for industrial applications. 
乳木果饼(SNC)是乳木果油提取过程中产生的固体残渣。SNC 可为细菌脂肪酶的生产提供低成本底物。固体发酵(SSF)的好处之一是产量更高。然而,在固态发酵法中,细菌生产脂肪酶的实例少于真菌。目前的研究旨在利用响应面法提高固态发酵法中 SNC 的细菌脂肪酶产量。从牛油果油厂污水池中分离出产脂酶细菌(LPB),并在三丁基琼脂平板上进行产脂酶筛选。采用 RSM 的盒式贝肯设计(BBD)研究了四个独立参数(温度、pH 值、含水量和生物表面活性剂)对脂肪酶产量的影响。结果表明,分离物 AO(假单胞菌)显示出最高的水解区 2(53 毫米)。R = 0.931 的二次回归结果表明,该模型的拟合效果最好,并预测出了具有显著性(P>0.05)的生长参数的最佳条件,包括温度为 40°C、含水量为 50%、生物表面活性剂为 2 mL,可产生 408.25 U/g 的实验脂肪酶。研究结果表明,该模型有助于预测在 SSF 中提高脂肪酶产量的最佳生长条件,而低成本的农业-工业 SNC 可能是一种具有工业应用前景的基质。
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引用次数: 0
Investigating Landsat data for continuous monitoring of ground electrical conductivity: implication for propagation at medium frequency in Ondo West 调查大地遥感卫星数据用于地面电导率连续监测:对翁多西部中频传播的影响
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.11
R.A. Adenodi, A. Ikusika
Ground electrical conductivity (GEC) has many applications, which makes it worthy of continuous research. Apart from the configurations  of the radio transmitter and receiver, it is a major determinant of the electric field intensity of radio waves in the Medium Frequency (MF)  band. It is usually measured in such a way that both the researcher and the measuring instrument are in direct contact with the ground,  and measurements are made at some predetermined constant intervals to ensure good spatial coverage and even spatial distribution.  The direct method becomes cumbersome when the field of study is large because the GEC over the licensed coverage area must be  known such that electric field intensity can be predicted. At the planning stage, this ensures all parts of the licensed region are reached by  useful signals that can suppress interference at all times and seasons. The study is motivated by the possibility of using Landsat to  estimate GEC. This study utilizes Landsat images to first estimate the normalized difference salinity index and then, with the aid of map  algebra, generate another raster 2 whose pixels' values are the GEC. The study covers a landmass of 967 km in the Ondo West Local  Government Area (OWLGA). During analysis, the GEC was divided into five classes. The results reveal that the ranges of very low, low,  moderate, high, and very high GEC are 0.124 to 0.437, 0.438 to 0.937, 0.938 to 1.635, 1.636 to 2.524, and 2.525 to 3.600 mS/m, which have  mean values of 0.346 ± 0.109, 0.754 ± 0.103, 1.071 ± 0.089, 1.351 ± 2 0.067, and 1.564 ± 0.106 mS/m, and are dispersed over 176.45, 202.24,  214.14, 374.17, and 228.56 km , respectively. The values of GEC, their respective coverage, and spatial distribution recorded in this study  are strong enough to sustain the propagation of useful electric field intensity over the entire landmass of Ondo West Local Government if  a medium wave is established. The study recommends that the change in GEC with time in this field be studied. 
地面导电率(GEC)有许多应用,因此值得继续研究。除了无线电发射机和接收机的配置外,它还是中频(MF)波段无线电波电场强度的主要决定因素。通常的测量方法是研究人员和测量仪器都与地面直接接触,并以预先确定的固定间隔进行测量,以确保良好的空间覆盖和均匀的空间分布。 当研究区域较大时,直接方法就会变得繁琐,因为必须知道许可覆盖区域内的 GEC,这样才能预测电场强度。在规划阶段,这可确保许可区域的所有部分都能收到有用的信号,从而在任何时间和季节都能抑制干扰。这项研究的动机是利用大地遥感卫星估算 GEC 的可能性。这项研究利用大地遥感卫星图像首先估算归一化差异盐度指数,然后借助地图代数生成另一个栅格 2,其像素值就是 GEC。研究覆盖翁多西部地方政府区(OWLGA)967 公里的陆地面积。在分析过程中,GEC 被分为五个等级。结果显示,极低、低、中、高和极高 GEC 的范围分别为 0.124 至 0.437、0.438 至 0.937、0.938 至 1.635、1.636 至 2.524 和 2.525 至 3.600 mS/m,其平均值分别为 0.346 ± 0.109、0.754 ± 0.103、1.071 ± 0.089、1.351 ± 2 0.067 和 1.564 ± 0.106 mS/m,分别分散在 176.45、202.24、214.14、374.17 和 228.56 km 范围内。本研究记录的 GEC 值、其各自的覆盖范围和空间分布足够强大,足以在建立中波的情况下维持有用电场强度在翁多西部地方政府整个陆地上的传播。该研究建议对该地区 GEC 随时间的变化进行研究。
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引用次数: 0
Production of vinegar from waste fruits using Acetobacter species 利用醋酸菌从废弃水果中生产醋
Pub Date : 2024-04-25 DOI: 10.4314/ijs.v26i1.14
S.K Paul, J. R. Wartu, A. Orukotan
Vinegar is an organic substance that contains about 5% acetic acid and is made from the oxidative fermentation of alcohol by acetic acid  bacteria. The aim of this study was to produce vinegar from the juices of waste fruits. Proximate and physicochemical analysis was  carried out on the juices of orange, sugarcane, tomato, and pineapple using standard techniques. Twenty-one (21) species of acetic acid  bacteria were isolated and identified using glucose-yeast-Calciumcarbonate (GYC) agar. The juices were fermented for 7 days using  Saccharomyces cerevisiae, and subsequently, for 28 days, four alcohol-tolerant Acetobacterspecies were selected and inoculated into the  fermenting media. The produced vinegar was sensory evaluated, following a nine-point hedonic scale. The antibacterial and antifungal  effects of the vinegar were tested on fresh cabbage at various contact times (0, 5, and 10 min) and concentrations (5, 10, and 15 % v/v).  The physicochemical parameters analyzed in fermentation using S. cerevisiae showed a decrease in pH, specific gravity, and brix, while  the percentage of alcohol content increased. As the fermentation using Acetobacter species progressed, pH further decreased, titratable  acidity increased in a range of 6.6-7.7%, and specific gravity also decreased while specific gravity increased in Acetic acid fermentation.  Sensory evaluation showed a score range of 5.7–6.8 for pungency, 6.8–7.4 for appearance, 6.4–7.9 for aroma, 5.8–7.4 for taste, and 6.9–7.9  for acceptability. The best results for bacterial and fungal loads 5 on cabbage were at a concentration of 15% at 10 min. Drastic reduction  of bacterial load was from 2.07×10 to 4 4 3 2.7×10 CFU/g, while fungal load reduced from 4.9×10 to 6.7× 10 CFU/g. To evaluate  significance, an ANOVA was performed (p<0.05) and Duncan's test was used for multiple comparisons. Vinegar was successfully tested to  be effective as an antimicrobial agent. Future research should look into process optimization, strain improvement, and the adoption of  agro-allied wastes for biotechnology.
醋是一种有机物质,含有约 5%的醋酸,由醋酸菌对酒精进行氧化发酵制成。本研究的目的是用废弃水果的果汁生产醋。采用标准技术对橙汁、甘蔗汁、番茄汁和菠萝汁进行了物性和理化分析。使用葡萄糖-酵母-碳酸钙(GYC)琼脂分离并鉴定了 21 种醋酸菌。使用酿酒酵母对果汁进行 7 天的发酵,然后在 28 天内筛选出 4 种耐受酒精的醋酸菌,并将其接种到发酵培养基中。按照九点享乐量表对生产的醋进行了感官评估。在不同的接触时间(0、5 和 10 分钟)和浓度(5、10 和 15 % v/v)下,测试了醋对新鲜卷心菜的抗菌和抗真菌效果。 在使用 S. cerevisiae 发酵过程中分析的理化参数显示,pH 值、比重和白利糖度都有所下降,而酒精含量的百分比则有所上升。随着醋酸菌发酵的进行,pH 值进一步下降,可滴定酸度在 6.6-7.7% 的范围内上升,比重也有所下降,而醋酸发酵时比重上升。 感官评价显示,辛辣味的评分范围为 5.7-6.8,外观评分范围为 6.8-7.4,香气评分范围为 6.4-7.9,口感评分范围为 5.8-7.4,可接受性评分范围为 6.9-7.9。在 10 分钟内浓度为 15%时,卷心菜上的细菌和真菌负载量 5 的结果最好。细菌量从 2.07×10 CFU/g 大幅减少到 4 4.3 2.7×10 CFU/g,真菌量从 4.9×10 CFU/g 减少到 6.7×10 CFU/g。为评估显著性,进行了方差分析(p<0.05),并使用邓肯检验进行多重比较。醋作为抗菌剂的有效性试验获得成功。未来的研究应着眼于工艺优化、菌株改良以及采用农业伴生废物的生物技术。
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引用次数: 0
期刊
Ife Journal of Science
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