Industrial Crops and Products最新文献_第2页

Analysis of the quality of Gastrodin elata Blume with different processing methods using a combination of NIR, FTIR and chemometrics 用近红外光谱、红外光谱和化学计量学相结合的方法分析天麻不同炮制方法的质量

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-23 DOI: 10.1016/j.indcrop.2026.122747

Yingfeng Zhong , Jieqing Li , Honggao Liu , Yuanzhong Wang

Post-harvest processing based on the steaming process significantly affects the quality of medicinal and edible Gastrodia elata Blume (G. elata). This study examined the effects of different processing methods on the bioactive components of G. elata. It proposed a process for identifying and evaluating the quality of various preparation methods using infrared spectroscopy combined with chemometrics. The results indicate that different processing methods led to an upward trend in parishin (A, B, C, E) and gastrodin, while 4-hydroxybenzyl alcohol showed a downward trend. The stir-frying (LP) preparation group exhibited the highest content of total characteristic components, reaching 11.93 mg/g. Furthermore, unlike previous studies that focused solely on quantitative composition analysis, we have been the first to employ two-dimensional correlation spectroscopy (2DCOS) to decipher the sequential changes in molecular dynamics of G. elata under different processing methods. This approach successfully visualized the continuous response of functional groups (such as O-H and C-H) to thermal agitation, providing new mechanistic insights into how processing alters product quality. It is noteworthy that the NIR-CARS-2DCOS-ResNet model achieved 100 % accurate identification across six processing methods, while also demonstrating improved efficiency compared to the full-spectrum ResNet model. For quantitative analysis, partial least squares regression (PLSR) models established between spectral data and HPLC reference values successfully predicted the content of individual characteristic components (except for parishin E). The residual prediction deviation (RPD) values for all optimal models under both strategies exceeded 2.00, indicating robust predictive performance, with the NIR strategy outperforming the FTIR strategy. This study provides a rapid, non-destructive, and effective strategy for both the authentication of processing methods and the quantitative prediction of key active constituents in G. elata, providing a powerful tool for its post-harvest quality control.

采后蒸制工艺对药用和食用天麻的品质有显著影响。本研究考察了不同加工方法对龙葵生物活性成分的影响。提出了一种利用红外光谱与化学计量学相结合的方法来鉴别和评价各种制备方法的质量。结果表明：不同炮制方法对巴利辛（A、B、C、E）和天麻素含量的影响呈上升趋势，4-羟基苄基醇的影响呈下降趋势。以炒（LP）制备组的总特征成分含量最高，达到11.93 mg/g。此外，与以往的研究不同，我们首次使用二维相关光谱（2DCOS）来破译不同处理方法下白杨分子动力学的序列变化。这种方法成功地可视化了官能团（如O-H和C-H）对热搅拌的连续响应，为加工如何改变产品质量提供了新的机制见解。值得注意的是，NIR-CARS-2DCOS-ResNet模型在六种处理方法中实现了100% %的准确率识别，同时与全谱ResNet模型相比，效率也有所提高。在定量分析方面，建立了光谱数据与HPLC参考值之间的偏最小二乘回归（PLSR）模型，成功预测了各特征成分的含量（parishin E除外）。两种策略下所有最优模型的残差预测偏差（RPD）值均超过2.00，表明预测性能稳健，且近红外策略优于FTIR策略。本研究提供了一种快速、无损、有效的方法，既可验证炮制方法，又可定量预测苦参关键活性成分，为苦参采后质量控制提供有力工具。

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引用次数: 0

Bioactive metabolites from the endophytic fungus Epicoccum sorghinum QA-20 isolated from Artemisia argyi with antibacterial activity against aquaculture pathogens and antioxidant potential 艾蒿内生真菌高粱表皮菌QA-20的生物活性代谢物及其对水产养殖病原菌的抑菌活性和抗氧化潜力

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-23 DOI: 10.1016/j.indcrop.2026.122751

Miao Liu , Qile Jiang , Bingui Wang , Xiaoming Li , Yao Pei , Huihan Tan , Yanhe Li , Xingyu Guo , Junming Tu , Jingjing Li , Xiaoshan Shi , Xian Xia

Artemisia argyi H. Lév. & Vaniot, an Asteraceae family herb, is extensively used in traditional Chinese pharmacopoeia and is a major local crop in Qichun County, possessing notable medicinal, economic, and cultural value. Its associated endophytes represent promising microbial candidates for natural product discovery. To investigate this potential, 40 fungal strains were isolated from A. argyi, and 12 with distinctive colony morphology were selected for scaled-down fermentation. Their EtOAc extracts were subsequently evaluated using HPLC, TLC, and antibacterial assays. Strain Epicoccum sorghinum QA-20, which displayed notable antibacterial effects, was chosen for further chemical investigation. Chromatographic separation from fermented broth extract yielded one previously undescribed polyketide, 7-methoxy-4,9-dimethyldibenzo[b,e][1,4]dioxin-2-ol (1), together with 12 known compounds (2–13). Structural elucidation was performed using NMR and/or mass spectrometry. Further structural confirmation of compound 1 was achieved through X-ray crystallographic analysis. All isolated compounds were investigated for antibacterial and antioxidant activities. Compounds 5–7 were effective in suppressing growth of pathogenic microbes, while compounds 1, 6, and 7 exhibited significant free radical scavenging activity. Compound 6 exhibited the broadest antibacterial spectrum, inhibiting all seven tested strains (MICs = 12.5–100 μg/mL); while compound 7 exhibited DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azino-bis) scavenging capacity (IC₅₀=15.81 ± 0.52and 32.95 ± 0.72 μg/mL, respectively), comparable to that of vitamin C (IC₅₀ = 11.62 ± 0.85 and 28.09 ± 1.40 μg/mL, respectively). This study demonstrates that endophytes derived from A. argyi are a valuable source of novel antibacterial and antioxidant natural products.

艾蒿。香叶是一种菊科草本植物，在中国传统药典中被广泛使用，是七春县的主要地方作物，具有显著的药用价值、经济价值和文化价值。其相关内生菌代表了天然产物发现的有希望的微生物候选者。为了研究这一潜力，从艾叶中分离出40株真菌，并选择了12株具有独特菌落形态的真菌进行规模化发酵。随后用高效液相色谱法、薄层色谱法和抗菌法对其乙酸乙酯提取物进行评价。选取抗菌效果显著的菌株高粱表皮菌QA-20进行进一步的化学研究。从发酵液中分离得到一种先前未描述的聚酮，7-甲氧基-4,9-二甲基二苯并[b，e][1,4]二恶英-2-醇(1)，以及12种已知化合物（2-13）。结构鉴定采用核磁共振和/或质谱法。通过x射线晶体学分析进一步证实了化合物1的结构。对分离得到的化合物进行了抗菌和抗氧化活性研究。化合物5 ~ 7具有抑制病原菌生长的作用，而化合物1、6、7具有明显的自由基清除活性。化合物6抗菌谱最广，对7株菌株均有抑制作用（mic = 12.5 ~ 100 μg/mL）；2-diphenyl-1-picrylhydrazyl虽然化合物7展出DPPH(2)和abt(2, 2’-azino-bis)清除能力(IC50 = 15.81 ±  0.52和32.95±0.72  μg / mL,分别),与维生素C (IC50 = 11.62 ±  0.85和28.09±1.40  μg / mL,分别)。本研究表明，艾叶内生菌是新型抗菌和抗氧化天然产物的重要来源。

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引用次数: 0

Phytotoxicity of high-dose graphene oxide in cotton seedlings: A multi-omics reveal of molecular and physiological responses 高剂量氧化石墨烯对棉花幼苗的植物毒性：分子和生理反应的多组学揭示

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-23 DOI: 10.1016/j.indcrop.2026.122702

Jianing Li , Zhiqiang Zhang , Xiangchi Lv , Yuzhi Zhang , Zhongying Ren , Xinyang Li , Wenqi Huo , Zongbin Ma , Wei Li , Wei Zhu , Wei Liu

Graphene oxide (GO), a widely used nanomaterial known for its unique physical and chemical properties, has raised increasing concerns about its potential environmental risks, particularly its phytotoxicity. Cotton, as an economically important non-food crop that does not enter the human food chain and has inherent advantages in soil pollution remediation, makes it an ideal model organism for assessing the environmental impact of GO. Here, we present the first multi-omics analysis integrating morpho-physiological, epigenetic, transcriptomic, and metabolomic approaches to elucidate the mechanisms of GO-induced stress in cotton seedlings. Exposure to high concentrations of GO (250 and 500 mg/L) caused dose-dependent growth inhibition, excessive accumulation of reactive oxygen species, and activation of antioxidant defenses. Transmission electron microscopy revealed the penetration of GO into root tissues and its translocation to aerial parts. Integrative multi-omics analyses revealed extensive transcriptional reprogramming, partially regulated by epigenetic modifications, accompanied by disruptions in key metabolic pathways such as phenylpropanoid and flavonoid biosynthesis, ABC transporters, and tryptophan metabolism. This study provides mechanistic insights into how cotton reallocates energy and metabolic resources from growth to defense under nanomaterial stress, establishing a framework for evaluating the sustainability and ecological safety of nanomaterials in agriculture.

氧化石墨烯（GO）是一种广泛使用的纳米材料，以其独特的物理和化学特性而闻名，其潜在的环境风险，特别是其植物毒性引起了越来越多的关注。棉花作为经济上重要的非粮食作物，不进入人类食物链，在土壤污染修复方面具有先天优势，是评估氧化石墨烯环境影响的理想模式生物。在这里，我们提出了第一个多组学分析，整合形态生理学，表观遗传学，转录组学和代谢组学方法来阐明氧化石墨烯诱导棉花幼苗应激的机制。暴露于高浓度氧化石墨烯（250和500 mg/L）会导致剂量依赖性生长抑制、活性氧的过度积累和抗氧化防御的激活。透射电镜显示氧化石墨烯在根组织中的渗透和向空中部位的转运。综合多组学分析揭示了广泛的转录重编程，部分由表观遗传修饰调节，伴随着关键代谢途径的破坏，如苯丙素和类黄酮的生物合成，ABC转运蛋白和色氨酸代谢。本研究提供了纳米材料胁迫下棉花如何将能量和代谢资源从生长重新分配到防御的机制见解，为评估纳米材料在农业中的可持续性和生态安全性建立了框架。

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引用次数: 0

Quantitative analysis and optimization of three film mulching methods and nitrogen application rates on maize yield and productivity 三种覆膜方式和施氮量对玉米产量和生产力的定量分析与优化

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-22 DOI: 10.1016/j.indcrop.2026.122691

Kang Zhao , Jing Jiang , Juanjuan Ma , Chaobo Zhang , Zhiping Yang

Understanding water-nitrogen interactions under mulched conditions and optimizing nitrogen application rates is crucial for ensuring maize productivity in semiarid regions. Through two-year field experiments (2021–2022), the effects of film mulching and nitrogen application on maize yield, water consumption, and water-nitrogen use efficiency were studied and optimal nitrogen application ranges were proposed. Three film mulching methods such as full-film double-ridge furrow sowing (FDS), flat film mulching (FCF), single ridge film mulching (SRC), in combination with four nitrogen application levels 0 (N0), 135 kg ha⁻¹ (N1), 180 kg ha⁻¹ (N2), and 225 kg ha⁻¹ (N3) were evaluated. Results demonstrated that FDS and SRC significantly improved maize yield and productivity while comparing to FCF. Furthermore, yield, water use efficiency (WUE), and nitrogen agronomic efficiency (NAE) demonstrated positive correlations with nitrogen application rate under FDS and SRC. In contrast, the nitrogen partial factor productivity (NPFP) related negatively with elevating nitrogen inputs under three methods. Under FCF, the WUE increased with increasing nitrogen application. Furthermore, the yield and NAE showed an increasing trend from N0 to N2 levels, but decreased at N3 levels. The ET_c at N3 for FDS, FCF, and SRC showed 0.30–4.49 %, 0.86–3.87 %, and 6.65–10.19 % reduction compared to other nitrogen treatments. Based on the results for yield, ET_c, and NPFP, the SRC mulching method was more suitable under low nitrogen levels (N0-N2). Under high nitrogen level (N3), the FDS mulching method performed better. Regression models showed that SRC and FDS can enable yield, WUE, NPFP, and dry matter mass to reach 80–100 %, 85–100 %, 90–100 %, and 95–100 % of their respective maximum values with the nitrogen ranges of 135–225 kg ha⁻¹, 164–225 kg ha⁻¹, 188–225 kg ha⁻¹, and 214–225 kg ha⁻¹. This study provides support for optimizing cultivation practices and determining appropriate nitrogen application ranges in semi-arid lands.

了解覆盖条件下的水氮相互作用，优化施氮量对保证半干旱区玉米产量至关重要。通过为期2年（2021-2022）的田间试验，研究了覆盖和施氮对玉米产量、耗水量和水氮利用效率的影响，并提出了最佳施氮范围。评价了全膜双垄沟播（FDS）、平膜覆盖（FCF）、单垄沟覆盖（SRC） 3种覆盖方式，以及4个施氮水平0 （N0）、135 kg ha−1 （N1）、180 kg ha−1 （N2）和225 kg ha−1 （N3）。结果表明，与FCF相比，FDS和SRC显著提高了玉米产量和生产力。产量、水分利用效率（WUE）和氮素农艺效率（NAE）与氮肥施用量呈显著正相关。在3种方法下，氮素偏要素生产率（NPFP）与氮投入的增加呈负相关。在FCF条件下，WUE随施氮量的增加而增加。产率和NAE在N0 ~ N2水平呈上升趋势，在N3水平呈下降趋势。与其他氮肥处理相比，FDS、FCF和SRC在N3条件下的ETc分别降低0.30 ~ 4.49 %、0.86 ~ 3.87 %和6.65 ~ 10.19 %。综合产量、ETc和NPFP结果，SRC覆盖方式在低氮水平（n0 ~ n2）下更为适宜。在高氮水平（N3）下，FDS覆盖效果较好。回归模型表明,SRC和FDS可以使产量、WUE, NPFP,和干物质质量达到80 - 100 %,85 - 100 %,90 - 100 %,和95 - 100年 %各自的最大值的氮范围135 - 225 公斤 公顷−1,164 - 225年 公斤 公顷−1,188 - 225年 公斤 公顷−1,和214 - 225年 公斤 公顷−1。本研究为半干旱区优化耕作方式和确定适宜的施氮范围提供了依据。

{"title":"Quantitative analysis and optimization of three film mulching methods and nitrogen application rates on maize yield and productivity","authors":"Kang Zhao , Jing Jiang , Juanjuan Ma , Chaobo Zhang , Zhiping Yang","doi":"10.1016/j.indcrop.2026.122691","DOIUrl":"10.1016/j.indcrop.2026.122691","url":null,"abstract":"<div><div>Understanding water-nitrogen interactions under mulched conditions and optimizing nitrogen application rates is crucial for ensuring maize productivity in semiarid regions. Through two-year field experiments (2021–2022), the effects of film mulching and nitrogen application on maize yield, water consumption, and water-nitrogen use efficiency were studied and optimal nitrogen application ranges were proposed. Three film mulching methods such as full-film double-ridge furrow sowing (FDS), flat film mulching (FCF), single ridge film mulching (SRC), in combination with four nitrogen application levels 0 (N0), 135 kg ha<sup>−1</sup> (N1), 180 kg ha<sup>−1</sup> (N2), and 225 kg ha<sup>−1</sup> (N3) were evaluated. Results demonstrated that FDS and SRC significantly improved maize yield and productivity while comparing to FCF. Furthermore, yield, water use efficiency (WUE), and nitrogen agronomic efficiency (NAE) demonstrated positive correlations with nitrogen application rate under FDS and SRC. In contrast, the nitrogen partial factor productivity (NPFP) related negatively with elevating nitrogen inputs under three methods. Under FCF, the WUE increased with increasing nitrogen application. Furthermore, the yield and NAE showed an increasing trend from N0 to N2 levels, but decreased at N3 levels. The ET<sub>c</sub> at N3 for FDS, FCF, and SRC showed 0.30–4.49 %, 0.86–3.87 %, and 6.65–10.19 % reduction compared to other nitrogen treatments. Based on the results for yield, ET<sub>c</sub>, and NPFP, the SRC mulching method was more suitable under low nitrogen levels (N0-N2). Under high nitrogen level (N3), the FDS mulching method performed better. Regression models showed that SRC and FDS can enable yield, WUE, NPFP, and dry matter mass to reach 80–100 %, 85–100 %, 90–100 %, and 95–100 % of their respective maximum values with the nitrogen ranges of 135–225 kg ha<sup>−1</sup>, 164–225 kg ha<sup>−1</sup>, 188–225 kg ha<sup>−1</sup>, and 214–225 kg ha<sup>−1</sup>. This study provides support for optimizing cultivation practices and determining appropriate nitrogen application ranges in semi-arid lands.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"240 ","pages":"Article 122691"},"PeriodicalIF":6.2,"publicationDate":"2026-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146023651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhizophagus irregularis inoculation reshapes root exudates, metabolomes and rhizosphere microbiomes to enhance perennial ryegrass adaptation under heat and cold stress 接种不规则食根菌可改变根分泌物、代谢组和根际微生物组，增强多年生黑麦草对冷热胁迫的适应能力

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-22 DOI: 10.1016/j.indcrop.2026.122734

Hongjian Wei , Yu Zhang , Xinjie Mao , Wenyuan He , Songkai Liao , Tingying Xu , Ming Tang , Hui Chen

Perennial ryegrass (Lolium perenne L.) holds promise for bioenergy and biochemical production, yet thermal extremes constrain its performance. Arbuscular mycorrhizal fungi (AMF) offer a sustainable avenue for stress mitigation, but the mechanisms by which arbuscular mycorrhizal (AM) symbiosis links organ-level metabolism, root exudation, and rhizosphere assembly under heat and cold stress remain poorly understood. Perennial ryegrass inoculated with Rhizophagus irregularis was subjected to heat stress and cold stress and profiled by GC–MS metabolomics, 16S/ITS sequencing, and soil physicochemical/enzymatic assays. Across both stresses, AMF stabilized growth, photosynthesis, and antioxidant status and increased soil available P, NH₄⁺–N, and C/N- cycling enzyme activities. Under heat stress, AMF triggered a root-centered program: amino acids (e.g., proline, asparagine) increased, selected organic acids (e.g., quinic and galacturonic acids) were reallocated, and root sterols/long-chain lipids rose; concurrently, exudates released fewer sugars but more nitrogenous metabolites. Under cold stress, AMF promoted a shoot-centered sugar regime (sucrose, glucose, fructose, trehalose) with concomitant accumulation of organic acids and membrane-related lipids that stabilize cellular structures; exudates shifted toward soluble sugars and nitrogenous compounds. In the rhizosphere, Bacillus and Massilia increased together with fungal Talaromyces, Trichoderma, and Penicillium under heat condition, whereas Bacillus and Pseudomonas increased and Fusarium declined under cold condition. Structural equation modeling (SEM) supported an AMF-triggered cascade from improved soil properties through exudates and community structure to organ metabolomes, with tolerance mediated mainly by root metabolism under heat condition and by shoot metabolism under cold condition. These results clarify how AMF coordinate plant metabolism and the rhizosphere microbiome to enhance heat and cold tolerance, and provide a basis for low-input, environmentally friendly turf and forage management.

多年生黑麦草（Lolium perenne L.）具有生物能源和生化生产的前景，但极端温度限制了其性能。丛枝菌根真菌（AMF）为缓解胁迫提供了一条可持续的途径，但在冷热胁迫下丛枝菌根（AM）共生与器官水平代谢、根渗出和根际组装之间的联系机制尚不清楚。采用GC-MS代谢组学、16S/ITS测序和土壤理化/酶学分析方法，对接种了不规则根噬菌的多年生黑麦草进行了热胁迫和冷胁迫研究。在这两种胁迫下，AMF稳定了生长、光合作用和抗氧化状态，并增加了土壤有效P、NH₄+ - N和C/N-循环酶的活性。在热胁迫下，AMF触发了以根为中心的程序：氨基酸（如脯氨酸、天冬酰胺）增加，选定的有机酸（如奎宁酸和半乳糖醛酸）重新分配，根固醇/长链脂质上升；同时，渗出液释放的糖更少，但氮代谢产物更多。在冷胁迫下，AMF促进了以芽为中心的糖体系（蔗糖、葡萄糖、果糖、海藻糖），并伴随有机酸和膜相关脂质的积累，从而稳定细胞结构；渗出物转向可溶性糖和含氮化合物。在根际，芽孢杆菌和Massilia在高温条件下增加，Talaromyces、Trichoderma和Penicillium在高温条件下增加，而Bacillus和Pseudomonas在低温条件下增加，Fusarium在低温条件下减少。结构方程模型（SEM）支持amf触发的从土壤特性改善到分泌物和群落结构再到器官代谢组的级联反应，其中耐受性主要由热条件下的根代谢和冷条件下的茎代谢介导。这些结果阐明了AMF如何协调植物代谢和根际微生物群，增强植物耐热性和耐寒性，为低投入、环境友好型草坪和牧草管理提供依据。

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引用次数: 0

Bioactive extract and component from fruit spikes of Prunella vulgaris L. induce apoptosis in non-small cell lung cancer: An integrative network pharmacology, molecular docking, and experimental validation approach 夏枯草果穗生物活性提取物和成分诱导非小细胞肺癌细胞凋亡：综合网络药理学、分子对接和实验验证方法

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-22 DOI: 10.1016/j.indcrop.2026.122697

Sin-Min Li , Yuen-Sing Lee , Kuan-Ying Huang , Jih-Jung Chen

Given the growing burden of lung cancer and the need for safer, mechanism-based interventions, functional natural products with radical-scavenging and apoptosis-modulating capacities have attracted increasing attention. Prunella vulgaris L., used in traditional Chinese medicine (TCM) as functional material, has been recognized for the health‑promoting properties. This study aimed to investigate the radical‑scavenging potential and apoptosis‑modulating effects of fruit spikes of P. vulgaris through an integrated network pharmacology, molecular docking, and experimental validation framework. Different solvent extracts and isolated compounds were prepared and screened for cytotoxicity against A549 cells and safety in HaCaT keratinocytes. The most active ethyl acetate extract (EtOAc) was further subjected to compounds isolation. Western blotting assessed apoptosis-related proteins, and molecular docking examined interactions. The results indicated the EtOAc extract possessed the highest total flavonoid content (TFC) and selective cytotoxicity toward A549 cells with minimal toxicity to HaCaT cells. Among the isolated compounds, quercetin (4), luteolin (5), rhein (6), and ursolic acid (7) exhibited notable cytotoxicity toward A549 cells, with quercetin (4) presenting the highest margin of safety (MOS). Western blot analysis revealed that the EtOAc extract and quercetin (4) modulated mitochondrial apoptosis by regulating B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), Caspase‑3, and protein kinase B (AKT). These findings provide mechanistic support for suggesting the fruit spikes of P. vulgaris may serve as a source of functional extracts and apoptosis‑inducing agents and worth further investigation for development of anticancer candidates.

鉴于肺癌的负担日益加重，以及对更安全、基于机制的干预措施的需求，具有清除自由基和调节细胞凋亡能力的功能性天然产物越来越受到关注。夏枯草（Prunella vulgaris L.）是一种具有保健作用的中药功能性材料。本研究旨在通过综合网络药理学、分子对接和实验验证框架，探讨黄皮果穗清除自由基和调节细胞凋亡的作用。制备了不同溶剂提取物和分离化合物，并对A549细胞的细胞毒性和HaCaT角质形成细胞的安全性进行了筛选。进一步对活性最强的乙酸乙酯提取物（EtOAc）进行化合物分离。Western blotting检测凋亡相关蛋白，分子对接检测相互作用。结果表明，EtOAc提取物具有最高的总黄酮含量和对A549细胞的选择性细胞毒性，对HaCaT细胞的毒性最小。槲皮素(4)、木犀草素(5)、大黄素(6)和熊果酸(7)对A549细胞具有显著的细胞毒性，其中槲皮素(4)的安全边际最高。Western blot分析显示，EtOAc提取物和槲皮素(4)通过调节B细胞淋巴瘤2 （Bcl - 2）、Bcl - 2相关X蛋白（Bax）、Caspase - 3和蛋白激酶B （AKT）来调节线粒体凋亡。这些研究结果为推测寻常花果穗可能作为功能性提取物和细胞凋亡诱导剂的来源提供了机制支持，值得进一步研究开发抗癌候选药物。

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引用次数: 0

Development of robust adhesion and water resistance soybean meal-based adhesive with long-lasting anti-mildew via in-situ constructed interpenetrating cross-linked network inspired by marine protein 受海洋蛋白质启发，通过原位构建互穿交联网络，开发具有持久抗霉性的强附着力和耐水性豆粕基粘合剂

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-22 DOI: 10.1016/j.indcrop.2026.122748

Shuchuan Cao , Siqi Zhao , Wenguang Zhou , Tao Liu , Ying Wang , Mingyang Bai , Xingong Li , Jianzhang Li

Soy protein-based adhesives are promising alternatives to petroleum-based options for industrial applications, but still face significant challenges such as weak initial adhesion, poor water resistance, limited application range, and susceptibility to mold. Inspired by the amino acid sequences of adhesion proteins in marine mussels and barnacles, we developed a protein adhesive with multifunctional properties by incorporating natural urushiol (U) and ten-alkyl dimethyl acrylamide bromide ammonium (AP) into the system to increase interface bonding sites. Additionally, in situ polymerization formed the long-chain polymer poly (AP-g-U), which functions as an adhesion protein to strengthen the cohesion of the adhesive cross-linked network. Benefiting from the poly (AP-g-U) and crosslinker 1,6-hexanediol diglycidyl ether (HDE), the synthetic adhesive demonstrated impressive pre-press bonding strength and wet bonding strength, improving from 0.64 MPa and 0 MPa to 1.08 MPa and 1.30 MPa compared to the unmodified soybean meal (SM) adhesive. Notably, the poly (AP-g-U) molecules containing numerous antibacterial quaternary ammonium salt groups anchored within the adhesive through intermolecular interactions formed a durable antibacterial network capable of providing sustained antibacterial effects upon contact. The synthetic adhesive exhibits exceptional mildew resistance for up to 150 days, surpassing most current soybean protein adhesives. Ultimately, this innovative design offers a practical approach to developing sustainable, high-performance, and environmentally friendly biomass-based adhesives for diverse industrial applications.

大豆蛋白基胶粘剂在工业应用中有望取代石油基胶粘剂，但仍面临着一些重大挑战，如初始粘结力弱、耐水性差、应用范围有限、易发霉等。受海洋贻贝和藤壶中粘附蛋白氨基酸序列的启发，我们通过在体系中加入天然漆酚(U)和十烷基二甲基丙烯酰胺溴化铵（AP）来增加界面键合位点，开发了一种具有多功能特性的蛋白质粘合剂。此外，原位聚合形成了长链聚合物poly (AP-g-U)，它作为一种粘附蛋白，增强了粘合剂交联网络的凝聚力。利用聚（AP-g-U）和交联剂1,6-己二醇二甘油酯醚（HDE），与未改性豆粕（SM）胶粘剂相比，合成的胶粘剂具有良好的预压和湿键强度，从0.64 MPa和0 MPa提高到1.08 MPa和1.30 MPa。值得注意的是，含有大量抗菌季铵盐基团的聚（AP-g-U）分子通过分子间相互作用锚定在粘合剂内，形成了一个持久的抗菌网络，能够在接触时提供持续的抗菌效果。合成胶粘剂表现出优异的抗霉性长达150天，超过了目前大多数大豆蛋白胶粘剂。最终，这种创新的设计为开发可持续、高性能和环保的生物质粘合剂提供了一种实用的方法，可用于各种工业应用。

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引用次数: 0

GMA-functionalized alkali lignin that can be covalently cross-linked is used in NR sustainable tire composites 将共价交联的gma功能化碱木质素应用于NR可持续轮胎复合材料中

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-22 DOI: 10.1016/j.indcrop.2026.122701

Yan-Ping Hu , Jing-Yan Liu , Xin-Li Jiang, De-Fa Hou , Xu Lin , Yun-Wu Zheng, Fu-Lin Yang , Yuan Zou, Hao Sun, Long Yang, Can Liu , Guan-Ben Du

Alkali-lignin (AL) is a commonly used biomass filler in natural rubber (NR) due to its abundant yield and aromatic structure. However, its compatibility with NR at the nanoscale is challenging due to its inherent polarity. In this study, glycidyl methacrylate (GMA) was used to modify AL to prepare modified alkali lignin (ALG), which introduced a polymerizable CC bond and reduced the polarity of AL. DFT (Density Functional Theory) results confirmed that the binding energy of NR/ALG was 2.57 % higher than that of NR/AL, and the compatibility was significantly improved. ALG can be dispersed with natural rubber latex at the nanoscale in aqueous solution. At the same time, ALG could photopolymerize with NR under UV light to form a wide range of lignin/NR cross-linking networks, which were further strengthened during the vulcanization process to achieve multiple enhancement effects. The tensile strength of NR@ALG reached 30.98 MPa, 27.9 % higher than that of pure NR. When co-doped with CB, NR@ALG@C has a higher tensile strength of 32.1 MPa, which is 13 % higher than that of NR@C. In addition, ALG also promoted the dispersion of CB in NR, and the particle size of NR@ALG@C decreased by 22.2 % compared with that of NR@C. The high E 'value in the DMA dynamic temperature scan further demonstrates the stability of the covalent structural network inside the composite. This environmental protection strategy successfully realizes the high-value utilization of industrial lignin, converting it into high-performance rubber composite fillers that are highly compatible with the goals of the circular economy.

碱木质素（AL）因其丰富的产率和芳香结构而成为天然橡胶（NR）中常用的生物质填料。然而，由于其固有的极性，它在纳米尺度上与NR的相容性具有挑战性。本研究采用甲基丙烯酸缩水甘油酯（GMA）对AL进行改性，制备了改性碱木质素（ALG），引入了可聚合的CC键，降低了AL的极性。密度泛函理论（DFT）结果证实，NR/ALG的结合能比NR/AL高2.57 %，相容性显著提高。ALG可与天然胶乳在纳米级水溶液中分散。同时，ALG可与NR在紫外光下光聚合，形成范围广泛的木质素/NR交联网络，并在硫化过程中得到进一步强化，达到多重增强效果。NR@ALG的抗拉强度达到30.98 MPa，比纯NR高27.9 %，与CB共掺时，NR@ALG@C的抗拉强度达到32.1 MPa，比NR@C高13 %。此外，ALG还促进了炭黑在NR中的分散，NR@ALG@C的粒径比NR@C降低了22.2% %。DMA动态温度扫描中的高E′值进一步证明了复合材料内部共价结构网络的稳定性。这一环保策略成功实现了工业木质素的高价值利用，将其转化为与循环经济目标高度契合的高性能橡胶复合填料。

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引用次数: 0

Genome-wide identification of MADS-box gene family members in poplar and functional characterization of PagMADS23a in the regulation of stomatal density 杨树MADS-box基因家族成员的全基因组鉴定及PagMADS23a调控气孔密度的功能表征

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-22 DOI: 10.1016/j.indcrop.2026.122735

Yufei Xia , Shenxiu Jiang , Yongyu Ren , Yuhang Zhang , Chenpu Jiang , Xiaofei Zhao , Xiangyang Kang

MADS-box family genes act as key regulators of plant growth, hormone signal transduction and secondary metabolism regulation. In this study, 150 MADS-box genes were identified in 84 K poplar (Populus alba × P. glandulosa). The phylogeny, gene structure, conserved motifs, collinearity, and cis-acting elements of these genes were analyzed, and the PagMADS-box genes were clustered into 17 groups. The results indicated that their evolution may be regulated by domain and motif distributions and influenced by multiple segmental and tandem duplication events. Meanwhile, based on differential gene expression analysis between tetraploid and diploid poplars, the PagMADS23a gene was identified and found to be significantly downregulated in the apical buds of tetraploid poplars, and its protein was localized to the nucleus. Transgenic analysis showed that overexpression of PagMADS23a increased stomatal density and promoted vegetative growth but reduced drought tolerance, whereas RNAi lines displayed opposite phenotypes. RT-qPCR results revealed that PagMADS23a markedly affected the expression of stomatal development–related genes, including EPF1, EPF2, EPFL9, SDD1, TMM, SPCH, MUTE, and FAMA. This study systematically characterized the 84 K poplar MADS-box transcription factor family, revealing the role of PagMADS23a in stomatal density regulation and providing insights into the drought resistance mechanisms of woody plants.

MADS-box家族基因是植物生长、激素信号转导和次生代谢的关键调控因子。本研究从84株 白杨（Populus alba × P. glandulosa）中鉴定出150个MADS-box基因。分析了这些基因的系统发育、基因结构、保守基序、共线性和顺式作用元件，将其聚类为17类。结果表明，它们的进化可能受到结构域和基序分布的调控，并受到多段重复和串联重复事件的影响。同时，通过对四倍体和二倍体杨树的差异基因表达分析，鉴定出PagMADS23a基因，发现该基因在四倍体杨树的顶芽中显著下调，其蛋白定位于细胞核。转基因分析表明，过表达PagMADS23a增加了气孔密度，促进了营养生长，但降低了耐旱性，而RNAi系表现出相反的表型。RT-qPCR结果显示，PagMADS23a显著影响气孔发育相关基因EPF1、EPF2、EPFL9、SDD1、TMM、SPCH、MUTE和FAMA的表达。本研究系统表征了84 K杨mads -box转录因子家族，揭示了PagMADS23a在气孔密度调控中的作用，为木本植物的抗旱机制提供了新的思路。

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引用次数: 0

Preparation and characterization of cellulose nanocrystals derived from Rosa roxburghii Tratt pomace: A novel strategy for the reuse of edible-medicinal homologous waste 刺梨果渣纤维素纳米晶的制备与表征：食药同源废弃物再利用的新策略

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-01-22 DOI: 10.1016/j.indcrop.2026.122744

Ying Li , Haijun Long , Huan Yu , Fangxia Zhang , Wulong Dan , Li Wang , Tao Yang , Yue Zhu , Ying Zhou , Zuhua Wang

Rosa roxburghii Tratt (RRT), a characteristic plant integrating edible and medicinal values, generates substantial fruit pomace as a by-product during juice processing. Although this biomass exhibits considerable potential for high-value utilization, its application in related fields remains inadequately explored. In this study, cellulose nanocrystals were successfully extracted from RRT pomace (RP-CNCs) through chemical treatment. The morphology and physicochemical characteristics of RP-CNCs were analyzed through SEM, TEM, XRD, FTIR, and TGA techniques, while their biocompatibility, safety profile, and biological functionality were assessed. The findings indicated that the prepared RP-CNCs exhibited mean diameter of (11.35 ± 6.02) nm, length of (520.00 ± 198.11) nm, and a zeta potential of (-28.81 ± 0.60) mV. Following multi-step chemical treatment, hemicellulose and lignin were effectively removed from the raw material, leading to an increase in crystallinity index (CrI) to (55.4 ± 2.6) %. Biological evaluation demonstrated that RP-CNCs exhibited excellent biocompatibility and safety within the concentration range of 0–400 μg/mL, with a hemolysis rate below 2.9 %. When incubated at 200 μg/mL for 72 h, RP-CNCs significantly enhanced the migration rate of L-929 cells to (88.9 ± 0.70) %. Moreover, 1 wt% RP-CNCs demonstrated suppressive effects on S. aureus, E. coli, and P. aeruginosa, whereas a 200 μg/mL concentration significantly inhibited NO secretion as an inflammatory mediator. This research proposes an innovative approach for the high-value utilization of RRT fruit residue, establishing a robust foundation for its potential applications in the biomedical field, particularly in the development of wound dressings.

刺梨（Rosa roxburghii Tratt， RRT）是一种集食用和药用价值于一体的特色植物，在果汁加工过程中会产生大量的果渣。尽管这种生物质显示出相当大的高价值利用潜力，但其在相关领域的应用仍未得到充分探索。本研究通过化学处理，成功地从RRT渣中提取纤维素纳米晶。通过SEM、TEM、XRD、FTIR和TGA等技术分析了rp - cnc的形态和理化特性，并对其生物相容性、安全性和生物功能进行了评价。结果表明，制备的RP-CNCs平均直径为（11.35 ± 6.02）nm，长度为（520.00 ± 198.11）nm， zeta电位为（-28.81 ± 0.60）mV，经多步化学处理后，半纤维素和木质素被有效去除，结晶度指数（CrI）提高到（55.4 ± 2.6）%。生物学评价表明，RP-CNCs在0 ~ 400 μg/mL浓度范围内具有良好的生物相容性和安全性，溶血率低于2.9 %。在200 μg/mL浓度下孵育72 h， RP-CNCs显著提高L-929细胞的迁移率，达到（88.9 ± 0.70）%。此外，1 wt%的RP-CNCs对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌有抑制作用，而200 μg/mL浓度的RP-CNCs则显著抑制作为炎症介质的NO分泌。本研究为RRT果渣的高价值利用提供了一种创新途径，为其在生物医学领域特别是创面敷料开发中的潜在应用奠定了坚实的基础。

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引用次数: 0