Pub Date : 2026-03-24DOI: 10.1016/j.indcrop.2026.123152
Wenwen Rong, Yuting Lin, Yingjian Zhang, Miao Ma, Anzhang Li
In licorice cultivation, excessive nitrogen fertilizer application has led to increasingly prominent issues, such as diminished quality and inadequate medicinal potency. Under 50% nitrogen reduction, this study used molybdenum trioxide (MoO3) and zinc oxide (ZnO) nanoparticles (NPs) at three concentration levels (0, 50, and 100 mg/L) in both single and combined application treatments to systematically investigate their effects on Glycyrrhiza glabra growth and quality. The results indicated that 50% nitrogen reduction significantly inhibited the growth of G. glabra and root nodule formation, while promoting the accumulation of glabridin, glycyrrhizic acid, liquiritin, glycyrrhetinic acid, and isoliquiritigenin in roots and enhancing the nitrogen-fixing capacity of root nodules. Notably, application of MoO3 NPs and ZnO NPs effectively alleviated the growth inhibition of G. glabra induced by nitrogen reduction, among which the 100 mg/L combined NPs treatment performed best: it not only significantly promoted aboveground growth of G. glabra, improved photosynthetic performance, increased the activity of key nitrogen-metabolizing enzymes, strengthened biological nitrogen fixation, and optimized root architecture, but also facilitated the accumulation of key secondary metabolites, with its efficacy even surpassing that of the full-nitrogen control (CK). Field experiments further verified that this optimal treatment increased per-hectare yield of G. glabra by 25.35% and total accumulation of key secondary metabolites by 20.34%–126.81% compared with CK. This study provides theoretical and practical bases for the sustainable and efficient cultivation of G. glabra under nitrogen reduction and the application of NPs in the green production of Chinese medicinal materials.
{"title":"Effects of nitrogen reduction combined with molybdenum trioxide and zinc oxide nanoparticles on the growth and quality of Glycyrrhiza glabra","authors":"Wenwen Rong, Yuting Lin, Yingjian Zhang, Miao Ma, Anzhang Li","doi":"10.1016/j.indcrop.2026.123152","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.123152","url":null,"abstract":"In licorice cultivation, excessive nitrogen fertilizer application has led to increasingly prominent issues, such as diminished quality and inadequate medicinal potency. Under 50% nitrogen reduction, this study used molybdenum trioxide (MoO<sub>3</sub>) and zinc oxide (ZnO) nanoparticles (NPs) at three concentration levels (0, 50, and 100 mg/L) in both single and combined application treatments to systematically investigate their effects on <em>Glycyrrhiza glabra</em> growth and quality. The results indicated that 50% nitrogen reduction significantly inhibited the growth of <em>G. glabra</em> and root nodule formation, while promoting the accumulation of glabridin, glycyrrhizic acid, liquiritin, glycyrrhetinic acid, and isoliquiritigenin in roots and enhancing the nitrogen-fixing capacity of root nodules. Notably, application of MoO<sub>3</sub> NPs and ZnO NPs effectively alleviated the growth inhibition of <em>G. glabra</em> induced by nitrogen reduction, among which the 100 mg/L combined NPs treatment performed best: it not only significantly promoted aboveground growth of <em>G. glabra</em>, improved photosynthetic performance, increased the activity of key nitrogen-metabolizing enzymes, strengthened biological nitrogen fixation, and optimized root architecture, but also facilitated the accumulation of key secondary metabolites, with its efficacy even surpassing that of the full-nitrogen control (CK). Field experiments further verified that this optimal treatment increased per-hectare yield of <em>G. glabra</em> by 25.35% and total accumulation of key secondary metabolites by 20.34%–126.81% compared with CK. This study provides theoretical and practical bases for the sustainable and efficient cultivation of <em>G. glabra</em> under nitrogen reduction and the application of NPs in the green production of Chinese medicinal materials.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"35 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147506817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The physical properties and starch quality of maize grains are critical for industrial processing, yet the combined effects of low light and low nitrogen (N) on these traits in different positional grains of hybrids with varying low-N-tolerance remain unclear. This two-year field experiment compared the low-N-tolerant hybrid ZhengHong 311 (ZH311) and the low-N-sensitive hybrid XianYu 508 (XY508), under natural light (CS) and 35% shading (SS), combined with low N (150 kg ha−1) and normal N (240 kg ha−1). We evaluated the grain morphological characteristics, test weight and hardness, starch and protein contents and their fractions, and starch granule morphology and pasting properties. The results showed that low light and low N significantly reduced soluble sugars, starch and protein contents and their fractions (particularly soluble sugars, albumin, and globulin), leading to decrease the grain size, test weight, and hardness. Under low light and low N, starch granules became more irregular and porous, and the proportion of large granules increased, which raised starch peak viscosity (PV), trough viscosity (TV), and final viscosity (FV), especially in XY508. Overall, apical grains were more negatively affected by low light and low N, particularly in XY508, whereas ZH311 maintained relatively stable grain quality under combined stress. This study provides insights for breeding maize hybrids resilient to low N and low light, and for developing management strategies to mitigate the effects of combined low light and low N stress.
玉米籽粒的物理性状和淀粉品质对工业加工至关重要，但低光低氮对不同耐低氮杂交种不同位置籽粒这些性状的综合影响尚不清楚。在自然光（CS）和35%遮荫（SS）条件下，采用低氮（150 kg ha−1）和正常氮（240 kg ha−1）组合，对耐低氮杂交种正红311 （ZH311）和低氮敏感杂交种鲜育508 （XY508）进行田间试验。对籽粒形态特征、试验重量和硬度、淀粉和蛋白质含量及其组分、淀粉颗粒形态和糊化性能进行了评价。结果表明，低光照和低氮显著降低了籽粒中可溶性糖、淀粉和蛋白质的含量及其组分（尤其是可溶性糖、白蛋白和球蛋白），导致籽粒大小、试重和硬度降低。在弱光低氮条件下，淀粉颗粒变得更加不规则和多孔，大颗粒比例增加，淀粉的峰值粘度（PV）、谷粘度（TV）和最终粘度（FV）升高，尤以XY508为明显。总体而言，低光低氮对籽粒质量的影响更大，特别是XY508，而ZH311在复合胁迫下保持了相对稳定的籽粒质量。本研究为培育抗低氮低光玉米杂交种，以及制定低光低氮联合胁迫的管理策略提供了参考。
{"title":"Low-nitrogen-tolerant maize hybrid can alleviate the effects of low light and low nitrogen stress on grain physical and starch quality","authors":"Pijiang Yin, Ye Tao, Wanyuan Liu, Xinglong Wang, Qinlin Liu, Fan Liu, Chengcheng Lyu, Chaode Liang, Tianqiong Lan, Dongju Fong, Fanlei Kong, Jichao Yuan","doi":"10.1016/j.indcrop.2026.123101","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.123101","url":null,"abstract":"The physical properties and starch quality of maize grains are critical for industrial processing, yet the combined effects of low light and low nitrogen (N) on these traits in different positional grains of hybrids with varying low-N-tolerance remain unclear. This two-year field experiment compared the low-N-tolerant hybrid ZhengHong 311 (ZH311) and the low-N-sensitive hybrid XianYu 508 (XY508), under natural light (CS) and 35% shading (SS), combined with low N (150 kg ha<sup>−1</sup>) and normal N (240 kg ha<sup>−1</sup>). We evaluated the grain morphological characteristics, test weight and hardness, starch and protein contents and their fractions, and starch granule morphology and pasting properties. The results showed that low light and low N significantly reduced soluble sugars, starch and protein contents and their fractions (particularly soluble sugars, albumin, and globulin), leading to decrease the grain size, test weight, and hardness. Under low light and low N, starch granules became more irregular and porous, and the proportion of large granules increased, which raised starch peak viscosity (PV), trough viscosity (TV), and final viscosity (FV), especially in XY508. Overall, apical grains were more negatively affected by low light and low N, particularly in XY508, whereas ZH311 maintained relatively stable grain quality under combined stress. This study provides insights for breeding maize hybrids resilient to low N and low light, and for developing management strategies to mitigate the effects of combined low light and low N stress.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"229 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147506845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-23DOI: 10.1016/j.indcrop.2026.123099
Ahsanul Amin, Mohammad Abraar Hossain, M. Ahsan Habib, Md. Abu Shaid Sujon, Mohammad Nasim, Md. Anisur Rahman
Bioinspired Bouligand architectures, characterized by helicoidal fiber alignment, offer exceptional damage tolerance and impact resistance in natural materials. In this work, a novel Bouligand-structured composite was developed using densified Gmelina arborea wood as a renewable fiber reinforcement and epoxy as the matrix. Composite laminates were fabricated through controlled densification, angular slicing, and epoxy-based lamination to replicate helicoidal stacking. Mechanical performance was systematically evaluated under various loading conditions, including flexural, compressive, impact, interlaminar shear, and moisture absorption.The Bouligand-structured composites exhibited pronounced mechanical enhancements compared with untreated wood and conventional laminate configurations. Impact strength increased by approximately ninefold relative to untreated wood, driven by effective energy-dissipation mechanisms including crack deflection, fiber pull-out, and interlaminar sliding. Flexural testing achieved an ultimate strength of 198.22 MPa and a modulus of 27.15 GPa, while interlaminar shear strength was maximized in Bouligand configurations at 90.08 MPa, indicating improved load transfer through twisted ply architecture. Anisotropic testing further revealed superior performance under radial loading. Microscopic observations confirmed that helicoidal ply orientation promotes discontinuous crack propagation and delayed catastrophic failure. Beyond mechanical performance, the composite exhibited reduced moisture uptake, low density (0.738 g·cm⁻³), and a substantially lower embodied carbon footprint: estimated at 50–60% lower than conventional carbon and glass-fiber composites. These results establish Bouligand-structured densified wood composites as a lightweight, sustainable, and mechanically efficient alternative for impact-prone structural applications such as transportation components and protective panels.
{"title":"Mechanical characterization of densified wood fiber composites for enhanced impact resistance through a bioinspired hierarchical architecture","authors":"Ahsanul Amin, Mohammad Abraar Hossain, M. Ahsan Habib, Md. Abu Shaid Sujon, Mohammad Nasim, Md. Anisur Rahman","doi":"10.1016/j.indcrop.2026.123099","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.123099","url":null,"abstract":"Bioinspired Bouligand architectures, characterized by helicoidal fiber alignment, offer exceptional damage tolerance and impact resistance in natural materials. In this work, a novel Bouligand-structured composite was developed using densified Gmelina arborea wood as a renewable fiber reinforcement and epoxy as the matrix. Composite laminates were fabricated through controlled densification, angular slicing, and epoxy-based lamination to replicate helicoidal stacking. Mechanical performance was systematically evaluated under various loading conditions, including flexural, compressive, impact, interlaminar shear, and moisture absorption.The Bouligand-structured composites exhibited pronounced mechanical enhancements compared with untreated wood and conventional laminate configurations. Impact strength increased by approximately ninefold relative to untreated wood, driven by effective energy-dissipation mechanisms including crack deflection, fiber pull-out, and interlaminar sliding. Flexural testing achieved an ultimate strength of 198.22 MPa and a modulus of 27.15 GPa, while interlaminar shear strength was maximized in Bouligand configurations at 90.08 MPa, indicating improved load transfer through twisted ply architecture. Anisotropic testing further revealed superior performance under radial loading. Microscopic observations confirmed that helicoidal ply orientation promotes discontinuous crack propagation and delayed catastrophic failure. Beyond mechanical performance, the composite exhibited reduced moisture uptake, low density (0.738 g·cm⁻³), and a substantially lower embodied carbon footprint: estimated at 50–60% lower than conventional carbon and glass-fiber composites. These results establish Bouligand-structured densified wood composites as a lightweight, sustainable, and mechanically efficient alternative for impact-prone structural applications such as transportation components and protective panels.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"16 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147495741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The poor bleachability of eucalyptus chemi-mechanical pulp (CMP) is widely recognized as a major challenge in the industry. In order to overcome these challenges, a novel and eco-friendly Hydro-Brightening Pretreatment (HBP) strategy is proposed, with the objective of enhancing the brightness of eucalyptus through the removal of bioactive lumen extractives (LEs) using hot water. The majority compositions of LEs are characterised as ellagic acid, taxifolin and caffeic acid. The disruption of chromophore formation is evidenced by the removal of these LEs. During the bleaching reaction, ellagic acid is converted into quinonoid rings via elimination reactions, and taxifolin is oxidised to form trihydroxy benzoic acid and dicarboxylic acids, while caffeic acid undergoes esterification. In comparison with the LEs retained strategy, the implementation of HBP resulted in an enhancement of the brightness of 2.53% ISO for Eucalyptus grandis CMP, and reached a brightness of 80.85% ISO for Acacia crassicarpa Benth. CMP. Concurrently, the LEs of Eucalyptus grandis manifest notable antioxidative capacity (IC50=36.13 for DPPH radical). Consequently, this work provides a strategy that simultaneously enhances CMP bleachability and explores the possibility of extracting high-value antioxidant products from LEs, offering new insights on improving CMP bleachability and potential added-value co-products.
{"title":"Hydro-brightening pretreatment removes antioxidant lumen extractives to enhance CMP bleachability","authors":"Ting Jiao, Jian Jiao, Hongjie Zhang, Yun Cheng, Yuqian Guo, Qingwen Tian, Xuelian Zhou, Aixiang Pan, Yongjun Deng, Guigan Fang","doi":"10.1016/j.indcrop.2026.123100","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.123100","url":null,"abstract":"The poor bleachability of eucalyptus chemi-mechanical pulp (CMP) is widely recognized as a major challenge in the industry. In order to overcome these challenges, a novel and eco-friendly Hydro-Brightening Pretreatment (HBP) strategy is proposed, with the objective of enhancing the brightness of eucalyptus through the removal of bioactive lumen extractives (LEs) using hot water. The majority compositions of LEs are characterised as ellagic acid, taxifolin and caffeic acid. The disruption of chromophore formation is evidenced by the removal of these LEs. During the bleaching reaction, ellagic acid is converted into quinonoid rings via elimination reactions, and taxifolin is oxidised to form trihydroxy benzoic acid and dicarboxylic acids, while caffeic acid undergoes esterification. In comparison with the LEs retained strategy, the implementation of HBP resulted in an enhancement of the brightness of 2.53% ISO for <em>Eucalyptus grandis</em> CMP, and reached a brightness of 80.85% ISO for <em>Acacia crassicarpa</em> Benth. CMP. Concurrently, the LEs of <em>Eucalyptus grandis</em> manifest notable antioxidative capacity (IC<sub>50</sub>=36.13 for DPPH radical). Consequently, this work provides a strategy that simultaneously enhances CMP bleachability and explores the possibility of extracting high-value antioxidant products from LEs, offering new insights on improving CMP bleachability and potential added-value co-products.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"14 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147496621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physiological combined with transcriptomics analysis provided new insights into the enhanced Cd stress resilience of Ricinus communis L. via castor bean meal biofertilizer","authors":"Pei Lei, Jierui Yang, Rui Shan, Yujing Bai, Yaxuan Jiang, Yong Zhao, Xue Lei, Xiaona Pei, Fenglan Huang, Ximei Ji, Fanjuan Meng","doi":"10.1016/j.indcrop.2026.123151","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.123151","url":null,"abstract":"","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"19 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147501673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To improve the quality of cigar tobacco leaves (CTLs) through microbial fermentation, an aroma-producing strain was identified as Bacillus safensis DMMJ-4, and the key fermentation parameters were optimized. Using gas chromatography–mass spectrometry (GC-MS) and electronic nose analysis combined with an orthogonal experimental design, the effects of fermentation temperature, inoculation concentration, and fermentation time on volatile flavor compounds (VFCs) were evaluated. The orthogonal test showed that the optimal process was CTLs fermented by Bacillus safensis DMMJ-4 at 40 °C with an inoculation concentration of 2 × 108 CFU/g for 21 days (P < 0.05). The inoculation concentration exerted the strongest influence on VFCs, followed by fermentation temperature and fermentation time. A total of 57 VFCs were common to the two groups, while 67 VFCs were detected in Bacillus safensis DMMJ-4 and 60 were found in CK group. The GC-MS results showed that the total VFCs content in the Bacillus safensis DMMJ-4 group increased by 103.68% compared to that in the CK group. Phenethyl alcohol, lauryl alcohol, megastigmatrienone, geranylacetone, β-damascenone, and β-dihydroionone were significantly increased, while β-dihydroionone (ROAV=100) and nonanal (ROAV=100) made the greatest contribution in Bacillus safensis DMMJ-4 and CK groups, respectively. The sensory quality of the CTLs in the Bacillus safensis DMMJ-4 group was significantly higher than that in the CK group. This study integrated functional microorganisms and fermentation processes to enhance CTLs quality, which also provides a reference and valuable insights for industrial production.
为提高雪茄烟叶的品质，鉴定了一株产香菌株萨芬芽孢杆菌DMMJ-4，并对其关键发酵参数进行了优化。采用气相色谱-质谱联用（GC-MS）和电子鼻分析相结合的正交试验设计，评价了发酵温度、接种浓度和发酵时间对挥发性风味化合物（vfc）的影响。正交试验结果表明，以萨氏芽孢杆菌DMMJ-4为培养基，接种浓度为2 × 108 CFU/g，在40℃条件下发酵21 d （P <； 0.05）为最佳工艺。接种浓度对vfc的影响最大，其次是发酵温度和发酵时间。两组共有57个vfc，其中萨氏芽孢杆菌DMMJ-4组共检测到67个vfc， CK组共检测到60个vfc。气相色谱-质谱分析结果表明，与CK组相比，芽孢杆菌DMMJ-4组总vfc含量提高了103.68%。苯乙醇、月桂醇、大草甲烯酮、香叶丙酮、β-马马酮和β-二氢离子酮含量显著增加，其中β-二氢离子酮（ROAV=100）和壬醛（ROAV=100）在沙芽孢杆菌DMMJ-4和CK组中贡献最大。萨芬芽孢杆菌DMMJ-4组ctl的感觉品质显著高于CK组。本研究将功能微生物与发酵工艺相结合，提高ctl的质量，为工业生产提供参考和有价值的见解。
{"title":"Effects of different fermentation parameters on physicochemical properties, flavor, and sensory of cigar tobacco leaves fermented with aroma-producing Bacillus safensis DMMJ-4","authors":"Haoyu Qi, Yuzong Zhao, Bingqing Hou, Xue Zhang, Fashi Peng, Haiyan Zhang, Yanqi Hu, Shengxiao Wang, Song Fang","doi":"10.1016/j.indcrop.2026.123104","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.123104","url":null,"abstract":"To improve the quality of cigar tobacco leaves (CTLs) through microbial fermentation, an aroma-producing strain was identified as <em>Bacillus safensis</em> DMMJ-4, and the key fermentation parameters were optimized. Using gas chromatography–mass spectrometry (GC-MS) and electronic nose analysis combined with an orthogonal experimental design, the effects of fermentation temperature, inoculation concentration, and fermentation time on volatile flavor compounds (VFCs) were evaluated. The orthogonal test showed that the optimal process was CTLs fermented by <em>Bacillus safensis</em> DMMJ-4 at 40 °C with an inoculation concentration of 2 × 10<sup>8</sup> CFU/g for 21 days (<em>P</em> < 0.05). The inoculation concentration exerted the strongest influence on VFCs, followed by fermentation temperature and fermentation time. A total of 57 VFCs were common to the two groups, while 67 VFCs were detected in <em>Bacillus safensis</em> DMMJ-4 and 60 were found in CK group. The GC-MS results showed that the total VFCs content in the <em>Bacillus safensis</em> DMMJ-4 group increased by 103.68% compared to that in the CK group. Phenethyl alcohol, lauryl alcohol, megastigmatrienone, geranylacetone, β-damascenone, and β-dihydroionone were significantly increased, while β-dihydroionone (ROAV=100) and nonanal (ROAV=100) made the greatest contribution in <em>Bacillus safensis</em> DMMJ-4 and CK groups, respectively. The sensory quality of the CTLs in the <em>Bacillus safensis</em> DMMJ-4 group was significantly higher than that in the CK group. This study integrated functional microorganisms and fermentation processes to enhance CTLs quality, which also provides a reference and valuable insights for industrial production.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"12 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147495744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-21DOI: 10.1016/j.indcrop.2026.123106
Huale Chen, Xinyue Ji, Tao Sun, Yan Wang, Chenxia Song, Shuxin Zhang, Weihao Song, Yuqi Sun, Juanfang Wang, Boqiang Tong, Yanjuan Mu, Yizeng Lu
The molecular mechanisms underlying the complex floral color patterns in Catalpa bungei, which are characterized by purplish-red spots and yellow nectar guides, are not fully understood. To elucidate these mechanisms, we integrated metabolomic and transcriptomic analyses of three accessions (P, B, and W) with distinct floral phenotypes. Phenotypic and colorimetric analyses revealed that accession P exhibits dense purplish-red spots and deep orange-yellow nectar guides, which correlated with the highest a* values in petals and highest b* values in nectar guides, whereas accession W shows sparse spots and pale yellow guides, and accession B displays an intermediate phenotype. Metabolomic profiling identified 2217 metabolites, highlighting the accumulation of specific anthocyanin derivatives, such as pelargonidin-3-p-coumarylglucoside in P and various acylated anthocyanins in B. The yellow nectar guides were primarily proposed to be attributed to flavonol glycosides. Transcriptomic analysis indicated that key anthocyanin pathway genes (e.g., CHS, CHI, F3H, DFR, ANS, UFGT) were significantly upregulated in P, whereas most FLS genes were downregulated. Weighted Gene Co-expression Network Analysis (WGCNA) identified eight modules (e.g., MEyellow, MEblue) strongly associated with color patterning, which are regulated by transcription factors (e.g., MYB, bHLH, WD40, bZIP) that show co-expression with modification genes such as UGT and AOMT. The expression patterns of 12 key genes were validated using qRT-PCR. This study provides the first comprehensive analysis of the metabolic and transcriptional networks controlling floral color patterning in C. bungei, proposing a synergistic regulatory mechanism and offering critical targets for molecular breeding.
{"title":"Integrated metabolomic and transcriptomic analysis reveals the metabolic and molecular mechanisms underlying floral color patterning in Catalpa bungei","authors":"Huale Chen, Xinyue Ji, Tao Sun, Yan Wang, Chenxia Song, Shuxin Zhang, Weihao Song, Yuqi Sun, Juanfang Wang, Boqiang Tong, Yanjuan Mu, Yizeng Lu","doi":"10.1016/j.indcrop.2026.123106","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.123106","url":null,"abstract":"The molecular mechanisms underlying the complex floral color patterns in <em>Catalpa bungei</em>, which are characterized by purplish-red spots and yellow nectar guides, are not fully understood. To elucidate these mechanisms, we integrated metabolomic and transcriptomic analyses of three accessions (P, B, and W) with distinct floral phenotypes. Phenotypic and colorimetric analyses revealed that accession P exhibits dense purplish-red spots and deep orange-yellow nectar guides, which correlated with the highest <em>a*</em> values in petals and highest <em>b*</em> values in nectar guides, whereas accession W shows sparse spots and pale yellow guides, and accession B displays an intermediate phenotype. Metabolomic profiling identified 2217 metabolites, highlighting the accumulation of specific anthocyanin derivatives, such as pelargonidin-3-<em>p</em>-coumarylglucoside in P and various acylated anthocyanins in B. The yellow nectar guides were primarily proposed to be attributed to flavonol glycosides. Transcriptomic analysis indicated that key anthocyanin pathway genes (<em>e.g.</em>, <em>CHS</em>, <em>CHI</em>, <em>F3H</em>, <em>DFR</em>, <em>ANS</em>, <em>UFGT</em>) were significantly upregulated in P, whereas most <em>FLS</em> genes were downregulated. Weighted Gene Co-expression Network Analysis (WGCNA) identified eight modules (<em>e.g.</em>, MEyellow, MEblue) strongly associated with color patterning, which are regulated by transcription factors (<em>e.g.</em>, MYB, bHLH, WD40, bZIP) that show co-expression with modification genes such as <em>UGT</em> and <em>AOMT</em>. The expression patterns of 12 key genes were validated using qRT-PCR. This study provides the first comprehensive analysis of the metabolic and transcriptional networks controlling floral color patterning in <em>C. bungei</em>, proposing a synergistic regulatory mechanism and offering critical targets for molecular breeding.","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"49 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147495736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-03-21DOI: 10.1016/j.indcrop.2026.123114
Xue Wang, Zhengnan Zhao, Xuemei Han, Si Liu, Tao Wang, Hengyue Zhang, Hongyan Sun
{"title":"Integrating transcriptome and metabolome analyses to reveal regulatory mechanism of physiological leaf scorch in Ginkgo biloba L.","authors":"Xue Wang, Zhengnan Zhao, Xuemei Han, Si Liu, Tao Wang, Hengyue Zhang, Hongyan Sun","doi":"10.1016/j.indcrop.2026.123114","DOIUrl":"https://doi.org/10.1016/j.indcrop.2026.123114","url":null,"abstract":"","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"16 1","pages":""},"PeriodicalIF":5.9,"publicationDate":"2026-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147495747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：