Industrial Crops and Products最新文献_第6页

Integrated metabolomics and transcriptomics reveal mechanisms underlying high-yield accumulation of 10-deacetylbaccatin III, baccatin III, and 10-deacetyltaxol in Taxus mairei twigs 综合代谢组学和转录组学揭示了10-去乙酰baccatin III、baccatin III和10-去乙酰紫杉醇在红豆杉（Taxus marei）枝条中高产积累的机制

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122875

Liming Huang , Feifan Zhao , Xiangfei Fu , Bin Wang , Longjiang Yu , Chunhua Fu

Taxus renewable twigs produce valuable taxanes, notably anticancer drug paclitaxel and its key intermediates, including 10-deacetylbaccatin III (10DAB), baccatin III (B-III), and 10-deacetyltaxol (10DAT). However, incomplete understanding of taxane biosynthetic networks and their regulatory mechanisms across species and developmental stages limits targeted genetic improvement of these medicinal plants. Here, we integrated metabolomics and transcriptomics to analyze taxane accumulation and associated biosynthetic gene expression patterns in three high-yielding (10DAB- or paclitaxel-rich) Taxus accessions (HZDNF, HZDDB, HZDMDY) twigs across developmental stages grown under uniform environmental conditions. Our study identified 55 distinct taxanes and 140 differentially expressed genes (DEGs) related to paclitaxel biosynthesis, enabling construction of relatively comprehensive metabolic networks and gene co-expression patterns. The HZDNF variety, particularly its young twigs (NF-new), showed exceptional production of most 6/8/6/4-taxanes (including 10DAB, B-III, and 10DAT), outperforming other varieties. This correlated with elevated expression of 42 potentially biosynthetic genes, including 10 putative rate-limiting enzyme genes. Furthermore, weighted gene co-expression network analysis (WGCNA) revealed 34 core DEGs from the above-mentioned 42 candidates, along with 13 potential master transcriptional factors (TFs). These TFs might enhance core DEGs expression via direct or indirect means, thereby boosting 10DAB/B-III/10DAT accumulation and high yield. Subsequent functional verification for two representative TFs of 13 candidates through transient overexpression, confirmed that two novel TFs (bHLH-16 and zf-HD-6) significantly enhance 10DAB/10DAT production by activating key DEGs (T7βOH-1, T10βOH-2 and BAPT-2). These findings advance understanding of taxane biosynthesis (especially 10DAB, B-III and 10DAT), providing targets for metabolic engineering of taxanes and Taxus germplasm improvement.

红豆杉可再生枝可生产有价值的紫杉烷，特别是抗癌药物紫杉醇及其关键中间体，包括10-去乙酰baccatin III (10DAB), baccatin III （B-III）和10-去乙酰紫杉醇（10DAT）。然而，对紫杉烷生物合成网络及其跨物种和发育阶段调控机制的不完全了解限制了这些药用植物的靶向遗传改良。在这里，我们整合代谢组学和转录组学分析了在统一环境条件下生长的三种高产（富含10DAB或紫杉醇）红豆杉材料（HZDNF， HZDDB， HZDMDY）不同发育阶段的紫杉醇积累和相关生物合成基因表达模式。我们的研究鉴定了55种不同的紫杉醇类和140个与紫杉醇生物合成相关的差异表达基因（DEGs），从而构建了相对全面的代谢网络和基因共表达模式。HZDNF品种，特别是其幼枝（NF-new），表现出大多数6/8/6/4-紫杉烷（包括10DAB、B-III和10DAT）的优异产量，优于其他品种。这与42个潜在的生物合成基因的表达升高相关，包括10个假定的限速酶基因。此外，加权基因共表达网络分析（WGCNA）从上述42个候选基因中发现了34个核心基因，以及13个潜在的主转录因子（TFs）。这些TFs可能通过直接或间接的方式增强核心DEGs的表达，从而促进10DAB/B-III/10DAT的积累和高产。随后通过瞬时过表达对13个候选TFs中的两个代表性TFs进行功能验证，证实了两个新的TFs （bHLH-16和zf-HD-6）通过激活关键DEGs （T7βOH-1、T10βOH-2和bpt -2）显著增强10DAB/10DAT的产生。这些发现促进了对紫杉烷（特别是10DAB、B-III和10DAT）生物合成的认识，为紫杉烷代谢工程和红豆杉种质改良提供了靶点。

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引用次数: 0

Multi-trait evaluation and selection of elite Cunninghamia lanceolata provenances for high-quality, large-diameter and knot-free timber 优质大径无结杉木优良种源的多性状评价与选择

IF 5.9 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122868

Fenglin Chen, Jun Peng, Yuhui Jiang, Lang Shi, Junjiao Ma, XiangQing Ma, Zhengying Cao, Ming Li

引用次数: 0

Overcoming impurity interference: Cotton fiber length measurement based on FI-Unet combined with CAGAN image segmentation and restoration 克服杂质干扰：基于FI-Unet结合CAGAN图像分割与恢复的棉纤维长度测量

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122733

Jinqiang Chang , Jianqiang Zhang , Liang Fang , Kang Liu , Hongwei Duan , Ruoyu Zhang

Cotton fiber length is not only a key indicator for determining cotton quality, but also an important basis for parameter control and grading during cotton processing. To address the challenges of accurately detecting fiber length of machine-picked cotton with high impurity content during pre-purchase inspections, a novel method combining FI-Unet for image segmentation and CAGAN for image restoration is proposed, which effectively reduces impurity interference. Models for predicting upper half mean length (UHML) and length uniformity (UI) are developed by extracting grayscale, pixel count, and contour features from transmission images of fibers and applying ensemble learning. The prediction results demonstrate strong performance of the model. The UHML prediction achieves an R² (coefficient of determination) of 0.977, a RMSE (root-mean-squared error) of 0.274 mm, and a MAE (mean absolute error) of 0.210 mm, while the UI prediction yields an R² of 0.811, a RMSE of 1.054 %, and a MAE of 0.998 %. For impurity segmentation, the FI-Unet model incorporates residual blocks and a multi-scale spatial attention mechanism during downsampling to enhance low-level feature extraction and region focus. During upsampling, an improved CBAM (convolutional block attention module) module establishes a multi-scale channel-space attention mechanism to refine detail perception. The model achieves an accuracy of 99.87 %, an F1-score of 97.59 %, and an MIOU of 97.60 % in image segmentation. Subsequent CAGAN-based restoration of impurity-occlued regions attains a PSNR (peak signal-to-noise ratio) of 39.16 and a SSIM (structural similarity index) of 0.97. Post-restoration analysis shows further improvements in UHML and UI prediction, with R² increasing by 0.017 and 0.059, RMSE decreasing by 0.097 mm and 0.153 %, and MAE decreasing by 0.044 mm and 0.161 %, respectively. This study results contribute greatly to the enhancement of the accuracy and robustness of cotton fiber length detection under varying impurity conditions.

棉纤维长度是决定棉花品质的关键指标，也是棉花加工过程中参数控制和分级的重要依据。针对机采高杂质棉在预检中难以准确检测出纤维长度的问题，提出了一种将FI-Unet图像分割与CAGAN图像恢复相结合的新方法，有效地降低了杂质干扰。通过从纤维传输图像中提取灰度、像素数和轮廓特征并应用集成学习，建立了上半平均长度（UHML）和长度均匀性（UI）的预测模型。预测结果表明，该模型具有较好的预测性能。uhtml预测的R2（决定系数）为0.977，RMSE（均方根误差）为0.274 mm， MAE（平均绝对误差）为0.210 mm，而UI预测的R2为0.811，RMSE为1.054 %，MAE为0.998 %。对于杂质分割，FI-Unet模型在降采样过程中结合了残差块和多尺度空间注意机制，以增强底层特征提取和区域聚焦。在上采样过程中，改进的CBAM（卷积块注意模块）模块建立了多尺度通道空间注意机制来细化细节感知。该模型在图像分割方面的准确率为99.87 %，f1分数为97.59 %，MIOU为97.60 %。随后基于cagan的杂质封闭区域恢复获得了39.16的PSNR（峰值信噪比）和0.97的SSIM（结构相似指数）。恢复后分析显示，UHML和UI预测进一步提高，R2分别提高0.017和0.059，RMSE分别降低0.097 mm和0.153 %，MAE分别降低0.044 mm和0.161 %。研究结果有助于提高不同杂质条件下棉纤维长度检测的准确性和稳健性。

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引用次数: 0

A modified seed coating agent enhanced cotton seedlings competitiveness via differential stomatal regulation and auxin-mediated leaf expansion 改良种包衣剂通过不同的气孔调节和生长素介导的叶片扩张增强棉花幼苗竞争力

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122867

Jianxin Zhao , Liwen Lu , Saierdaer Reyimu , Shen Qiu , Xiaofen Yao , Dandan Chen , Wajid Mahboob , Jie Peng , Xiaoqi Xie , Zhao Zhang , Guozheng Yang

For direct-seeded cotton production following wheat or rapeseed harvested in the Yangtze River Basin, competition of space and nutrients from regenerated seedlings of previous crops (they are weeds actually here) hinders cotton seedlings’growth. This study aims to elucidate the mechanisms of potential removal of the constrain by modifying a commercialized seed coating agent with different exogenous substances (potassium dihydrogen phosphate (PDP), glucose (Glc), and diethyl aminoethyl hexanoate (DA-6)). Treatments included a water control (CK), a basic seed coating agent (BSC), and BSC supplemented with graded levels of PDP (1–8 g L⁻¹), Glc (1.6–6.4 g L⁻¹), or DA-6 (5–320 mg L⁻¹). Effects on seed emergence, seedling growth, photosynthetic traits, and leaf anatomy were assessed through indoor, pot, and field experiments. Results indicated that 4 g L⁻¹ PDP (PDP3) and 1.6 g L⁻¹ Glc (Glc1) were more effective than other treatments, though no synergistic effects occurred when combined. PDP3 markedly improved seed emergence (93.33 %) and enhanced seedling leaf area, height, and biomass. It maintained auxin homeostasis by suppressing IAA oxidase (IAAO) activity, promoted upper epidermal cell expansion, and differentially regulated stomatal distribution, significantly increasing stomatal density on the upper epidermis by 37.0 % while decreasing it on the lower epidermis. PDP3 also elevated pigment content, ribulose-1,5-bisphosphate carboxylase (RuBPCase) activity, and net photosynthetic rate (13.70 % higher than CK), resulting in greater accumulation of soluble sugars and sucrose. Field trials further showed that PDP3-treated cotton sustained larger leaf area and higher photosynthetic efficiency in later stages, increasing seed cotton and fiber yields by 18.00 % and 24.00 %, respectively. These findings demonstrate that the modified coating agent, especially PDP3, promotes vigorous seedling establishment by orchestrating a coordinated physiological program involving auxin homeostasis, optimized stomatal anatomy, and enhanced photosynthetic capacity, thereby providing a novel strategy to overcome the early competition bottleneck in direct-seeded cotton systems.

在长江流域，小麦或油菜籽收获后直接播种棉花，以前作物的再生苗（在这里实际上是杂草）的空间和养分竞争阻碍了棉花幼苗的生长。本研究旨在阐明用不同外源物质（磷酸二氢钾（PDP）、葡萄糖（Glc）和己酸二乙基氨基乙酯（DA-6））修饰一种商品化种子包衣剂的潜在去除约束的机制。处理包括水分控制（CK），基本包衣剂（BSC）和BSC， BSC补充了分级水平的PDP(1 - 8 g L−1),Glc（1.6-6.4 g L−1）或DA-6（5-320 mg L−1）。通过室内、盆栽和田间试验，评价了不同处理对种子出苗、幼苗生长、光合性状和叶片解剖结构的影响。结果表明，4 g L−1 PDP （PDP3）和1.6 g L−1 Glc （Glc1）比其他处理更有效，但联合使用时没有增效作用。PDP3显著提高了种子出苗率（93.33 %），提高了幼苗叶面积、叶高和生物量。通过抑制IAA氧化酶（IAAO）活性维持生长素稳态，促进上表皮细胞增殖，差异调节气孔分布，显著提高上表皮气孔密度37.0 %，降低下表皮气孔密度。PDP3还提高了色素含量、核酮糖-1,5-二磷酸羧化酶（RuBPCase）活性和净光合速率（比CK高13.70 %），导致可溶性糖和蔗糖的积累更多。田间试验进一步表明，pdp3处理棉花后期叶面积增大，光合效率提高，籽棉和纤维产量分别提高18.00 %和24.00 %。这些发现表明，改性包衣剂，特别是PDP3，通过协调生长素稳态、优化气孔解剖结构和增强光合能力的生理程序来促进幼苗茁壮成长，从而为克服棉花直接播种系统的早期竞争瓶颈提供了一种新的策略。

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引用次数: 0

Tiered valorization of corn straw-deer manure waste: Enhanced button mushroom quality coupled with functional characterization of stage-specific substrate for targeted industrial use 玉米秸秆-鹿粪废物的分层增值：提高钮扣菇质量，结合针对工业用途的特定阶段基质的功能表征

IF 5.9 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122878

Keqing Qian, Peng Dong, Arman Alim, Wenying Su, Weijian Li, Ze Liu, You Li, Zhengxiang Qi, Haiyan Lv, Xiaohui Yuan, Yu Li, Xiao Li, Bo Zhang

引用次数: 0

Unlocking the potential oil palm (Elaeis guineensis Jacq.) leaf in wound care management: A review on phytochemicals, bioactivities and underlying mechanism pathways in wound healing 油棕叶在伤口护理管理中的潜力：植物化学物质、生物活性及其在伤口愈合中的潜在机制途径综述

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122863

Mohamad Shazeli Che Zain

Wound care management is a significant challenge in clinical practice, prompting the exploration of novel and effective treatments. The oil palm (Elaeis guineensis Jacq.), primarily cultivated for its oil, shows promising potential beyond its traditional uses. This review explores the phytochemicals present in oil palm leaves, extraction technologies, their biological activities, and underlying mechanism pathways in wound healing. Through an extensive review of current literature, key compounds such as flavonoids, phenolic acid glycosides, organic acids, amine and amino acids, and carbohydrates are identified. Numerous studies have reported that catechin, apigenin and luteolin derivatives possess wound-healing properties due to their well-acclaimed anti-inflammatory, angiogenesis, re-epithelialization, and antioxidant effects. They have been forecast to influence the wound healing process by expressing biomarkers associated with various pathways, including nuclear factor kappa b (NF-κB), Wnt/β-catenin, transforming growth factor-beta (TGF-β), NF-E2-related factor 2/antioxidant responsive element (Nrf2/ARE) pathways, among others. Importantly, nanotechnology-based formulations such as nanoemulsions and nanogels have demonstrated enhanced targeted delivery of these bioactive extracts, improving their solubility, stability, bioavailability, and site-specific accumulation at the wound bed. The incorporation of nanotechnology in wound care product development thus represents a promising strategy to maximize the therapeutic potential of oil palm leaf phytochemicals. These advancements support the development of precise, next-generation herbal wound care therapies that are both effective and safe. Opportunity and challenges to maximize the exploitation of this leaf are also highlighted at the end of this review.

伤口护理管理是临床实践中的一个重大挑战，促使探索新颖有效的治疗方法。油棕（Elaeis guineensis Jacq.），主要是为了它的油而种植的，在其传统用途之外显示出有希望的潜力。本文综述了油棕叶中的植物化学物质、提取技术、生物活性及其在创面愈合中的潜在机制途径。通过对当前文献的广泛回顾，确定了黄酮类化合物、酚酸苷、有机酸、胺和氨基酸以及碳水化合物等关键化合物。大量研究报道，儿茶素、芹菜素和木犀草素衍生物具有良好的抗炎、血管生成、再上皮化和抗氧化作用，具有伤口愈合特性。据预测，它们通过表达与各种途径相关的生物标志物来影响伤口愈合过程，包括核因子κ b （NF-κB）、Wnt/β-catenin、转化生长因子-β (TGF-β)、NF- e2相关因子2/抗氧化反应元件（Nrf2/ARE）途径等。重要的是，基于纳米技术的配方，如纳米乳液和纳米凝胶，已经证明了这些生物活性提取物的靶向递送，提高了它们的溶解度、稳定性、生物利用度和伤口床的部位特异性积累。因此，在伤口护理产品开发中纳入纳米技术代表了一种有前途的策略，可以最大限度地发挥油棕叶植物化学物质的治疗潜力。这些进步支持精确，下一代草药伤口护理疗法的发展，既有效又安全。最大限度地利用这片叶子的机遇和挑战也在本文的最后强调。

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引用次数: 0

Genome-wide analysis of cytochrome P450 genes discovers two oxidases responsible for the biosynthesis of oxindole alkaloids in Uncaria rhynchophylla 细胞色素P450基因的全基因组分析发现了两种氧化酶，这些氧化酶负责钩藤氧化吲哚生物碱的生物合成

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122877

Hao-cheng Lou , Jia-shun Yang , Hong Zhang , Chao Li , Xiao-jun Pan , Jia-ning Pan , Shi-zan Cao , Li-shang Dai , Zhi-gang Wu

Uncaria rhynchophylla (Gou-teng, UR) produces numerous structurally diverse tetracyclic monoterpene oxindole-type alkaloids (tMOAs) with notable pharmacological activities. Cytochromes P450 (CYPs) play crucial roles in generating the structural diversity of specialized metabolites. However, the CYP superfamily and its biochemical function in tMOAs biosynthesis in UR remains poorly characterized, largely due to the complexity of P450 genes. In this study, a total of 460 UrCYP genes were identified in the UR genome and clustered into nine clans and 43 families by a comprehensive genome-wide analysis. Furthermore, phylogenetic relationships, gene structure, conserved motifs, and duplication events driving the expansion of these UrCYPs were revealed, respectively. Using gene coexpression analysis and yeast functional assays, two tandemly duplicated CYP71 members were discovered to catalyze the regio-specific C-2’ oxindole and rearrangement of tetracyclic corynanthe-type intermediates (hirsutine, hirsuteine) to form tMOAs, including rhynchophylline, isorhynchophylline, corynoxeine, and isocorynoxeine. Meanwhile, overexpression and RNA interference of UrCYP71A22.4 and UrCYP71A22.5 substantially increased and decreased the accumulation of these oxindole alkaloids in hairy roots, respectively. Subcellular localization revealed that UrCYP71A22.4/5-eGFP fusion proteins localize to endoplasmic reticulum membranes. Docking analysis identified four conserved candidate residues (S134/130, D319/318, R447/459, P450/462) surrounding UrCYP71A22.4/5 binding pockets that likely serve as key active sites for 2-oxindole structure formation. Together, this study not only provides critical insights into the previously unknown biosynthesis of tMOAs in UR but also offers a potential strategy for the sustainable production of these pharmaceutically valuable alkaloids.

钩藤（Uncaria rhynchophylla, gouteng， UR）具有多种结构多样的四环单萜氧吲哚类生物碱（tMOAs），具有显著的药理活性。细胞色素P450 （CYPs）在产生特殊代谢物的结构多样性中起着至关重要的作用。然而，由于P450基因的复杂性，CYP超家族及其在UR中tMOAs生物合成中的生化功能仍然知之甚少。本研究通过全面的全基因组分析，在UR基因组中共鉴定出460个UrCYP基因，并将其聚类为9个氏族和43个家族。此外，还揭示了系统发育关系、基因结构、保守基序和驱动这些UrCYPs扩增的复制事件。通过基因共表达分析和酵母功能分析，发现两个连续重复的CYP71成员催化区域特异性的C-2 '氧化吲哚和四环堇青素型中间体（hirsutin, hirsuteine）重排形成tmoa，包括rhynchophyline， isorhynchophyline， corynoxeine和isocorynoxeine。同时，UrCYP71A22.4和UrCYP71A22.5的过表达和RNA干扰分别显著增加和减少了毛状根中这些氧吲哚类生物碱的积累。亚细胞定位显示UrCYP71A22.4/5-eGFP融合蛋白定位于内质网膜。对接分析发现urcyp71a21.4 /5结合袋周围有4个保守的候选残基（S134/130、D319/318、R447/459、P450/462），可能是2-氧吲哚结构形成的关键活性位点。总之，这项研究不仅为以前未知的tMOAs在UR中的生物合成提供了重要的见解，而且为这些具有药用价值的生物碱的可持续生产提供了潜在的策略。

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引用次数: 0

Astragalus polysaccharides modulate gut microbial interaction and bile acid metabolism of postpartum dairy cows 黄芪多糖对产后奶牛肠道微生物相互作用及胆汁酸代谢的调节作用

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122861

Zhengzhong Luo , Kang Yong , Kang Zhang , Yixin Huang , Zheng Zhou , Xueping Yao , Liuhong Shen , Shumin Yu , Jianxi Li , Suizhong Cao

Gut microbiota is a symbiotic ecosystem, yet how polysaccharides regulate gut microbial interactions and the related host co-metabolism in dairy cows, particularly regarding bile acids, remain unclear. In postpartum cows, Astragalus polysaccharides (APS) supplementation reduced key biomarkers of metabolic stress, including β-hydroxybutyrate, triglycerides, interferon-γ, and TNF-α. Although fecal microbial diversity did not show significant changes, APS markedly altered interactions within the gut microbiota, improving cohesion and stability of co-occurrence network, such as a higher clustering coefficient, greater core modularization, and enhanced robustness, with reduced average path length and vulnerability. APS elevated bile acids such as tauroursodeoxycholic acid, independent of changes in related taxa abundance. Moreover, microbial functional gene networks exhibited strong correlations with bile acid metabolism after APS treatment. These findings demonstrate that APS enhances microbial network stability and bile acid–related host co-metabolism, alleviating postpartum metabolic stress in dairy cows.

肠道菌群是一个共生生态系统，但多糖如何调节奶牛肠道微生物的相互作用和相关的宿主共代谢，特别是胆汁酸，尚不清楚。在产后奶牛中，添加黄芪多糖（APS）可降低代谢应激的关键生物标志物，包括β-羟基丁酸、甘油三酯、干扰素-γ和TNF-α。虽然粪便微生物多样性没有明显变化，但APS显著改变了肠道微生物群之间的相互作用，提高了共发生网络的凝聚力和稳定性，如更高的聚类系数，更大的核心模块化，增强了鲁棒性，减少了平均路径长度和脆弱性。APS升高了牛磺酸去氧胆酸等胆汁酸，独立于相关分类群丰度的变化。此外，APS处理后，微生物功能基因网络与胆汁酸代谢表现出很强的相关性。由此可见，APS可增强奶牛微生物网络稳定性和与胆汁酸相关的宿主共代谢，缓解奶牛产后代谢应激。

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引用次数: 0

Whole-genome resequencing reveals the genetic diversity and adaptive evolution of northern Chinese populations of Quercus mongolica 全基因组重测序揭示了中国北方蒙古栎居群的遗传多样性和适应性进化

IF 5.9 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122880

Min Jiang, Xinman Li, Shuiqing Yu, Junjie Ren, Wei Liu, Yuhan Sun, Zijie Zhang, Jinmao Wang, Minsheng Yang

引用次数: 0

Multi-omics integration uncovers terpenoid-centered plant-microbe interactions underpinning the adaptive resilience of Thuja sutchuenensis 多组学整合揭示了以萜类化合物为中心的植物-微生物相互作用，支撑了苏楚树的适应能力

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products

Pub Date : 2026-02-10 DOI: 10.1016/j.indcrop.2026.122873

You-wei Zuo , Yang Peng , Sheng-mao Zhou , Shi-qi You , Yan-ru Chen , Sheng-qiao Liu , Quan Yang , Ling-xiang Yang , Hong-ping Deng

Understanding the interaction among metabolites, microbes, and gene regulation is crucial for deciphering plant adaptation in extreme habitats. Here, we integrated metabolomic, metagenomic, and transcriptomic analyses to investigate the terpenoid-centered plant-microbe interactions of the endangered conifer Thuja sutchuenensis across natural, restored, and cultivated populations. LC-MS/MS profiling revealed that natural populations accumulate higher levels of terpenoids, flavonoids, and phenolic derivatives. Metagenomic analysis showed that Actinobacteria- and Bradyrhizobium-dominated microbial communities were functionally enriched in terpenoid-backbone, monoterpenoid, and xenobiotic-degradation pathways, indicating strong microbial specialization within the limestone rhizosphere. Transcriptomic profiling of roots showed coordinated alterations of key pathway genes (HMGR, DXS, SQE1, SS1, and VTE4). These transcriptional changes were accompanied by the expression of downstream oxidoreductases and cytochrome P450s, supporting terpenoid diversification and antioxidant capacity. Cross-omics correlation networks revealed strong associations among terpenoid-related genes, metabolites, and Actinobacteria taxa, highlighting a metabolic feedback loop that links microbial symbiosis with host secondary metabolism. Integrative analyses further suggested that wild populations maintain a more complex and functionally interconnected host-microbe system than cultivated ones. Collectively, these findings uncover a finely tuned plant-microbe-metabolite regulatory network that underpins the adaptive plasticity of T. sutchuenensis and provide a molecular framework for microbiome-assisted conservation, restoration, and sustainable management of this relict conifer species.

了解代谢物、微生物和基因调控之间的相互作用对于破译植物在极端栖息地的适应至关重要。在这里，我们整合了代谢组学、宏基因组学和转录组学分析，研究了濒危针叶树苏川树（Thuja sutchuenensis）在自然、恢复和栽培种群中以萜类化合物为中心的植物-微生物相互作用。LC-MS/MS分析显示，天然种群积累了较高水平的萜类、类黄酮和酚类衍生物。宏基因组分析表明，放线菌和慢生根瘤菌主导的微生物群落在萜类主干、单萜类和外源降解途径上功能丰富，表明石灰岩根际微生物具有很强的特化性。根系转录组学分析显示，关键通路基因（HMGR、DXS、SQE1、SS1和VTE4）发生了协同改变。这些转录变化伴随着下游氧化还原酶和细胞色素p450的表达，支持萜类化合物多样化和抗氧化能力。交叉组学相关网络揭示了萜类相关基因、代谢物和放线菌类群之间的强关联，突出了微生物共生与宿主次生代谢之间的代谢反馈回路。综合分析进一步表明，野生种群比栽培种群保持更复杂和功能互联的宿主-微生物系统。总的来说，这些发现揭示了一个精细调节的植物-微生物-代谢物调节网络，该网络支撑了苏川针叶树的适应性可塑性，并为微生物组辅助的苏川针叶树的保护、恢复和可持续管理提供了一个分子框架。

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引用次数: 0