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Vapor-liquid equilibrium measurements and thermodynamic performance assessment on eco-friendly working pairs of R1270+DMETrEG and R1270+NMP for Absorption Refrigeration System 吸收式制冷系统R1270+DMETrEG和R1270+NMP环保工作对汽液平衡测量及热力学性能评价
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-21 DOI: 10.1016/j.ijrefrig.2026.01.028
Deheng Kong , Sai Zhou , Mingjing Fan , Qian Ning , Qinglu Song , Dechang Wang , Guogeng He
As an environmentally sustainable refrigeration technology, the absorption refrigeration cycle (ARC) has garnered increasing attention, and research on the vapor–liquid equilibrium (VLE) of novel working pairs is of great significance for the promotion and development of ARC. This article innovatively measured the VLE of hydrocarbon (HC) refrigerant R1270 in organic solvents DMETrEG and NMP in the temperature range of 303.15 K to 353.15 K, and used the non-random two-liquid (NRTL) model to fit the experimental data and evaluate their performance in the ARC. For the R1270+DMETrEG, the average absolute relative deviation (AARD) and maximum absolute relative deviation (MARD) are 0.8318 % and 2.8169 %, respectively. For the R1270+NMP, the corresponding values are 1.4472 % and 3.1676 %. In the thermodynamic analysis of the ARC, the maximum coefficient of performance (COP) of the R1270+DMETrEG working pair is 0.327, which is higher than 0.313 of the R1270+NMP. Compared to COP values predicted using a UNIFAC-DMD model, the COP calculated from experimental VLE data is approximately 0.02–0.08 lower, although the overall trend remains consistent. In all cases, the COP of R1270+DMETrEG is higher than that of R1270+NMP, in both experimental and predicted results. Based on the experimental data, the COP of the R1270 working pairs is 0.01–0.1 lower than that of R1234yf and R1234ze(E). At constant generation temperature, both working pairs exhibit similar COP trends as a function of evaporation and condensation temperatures. Analysis of excess properties shows that the excess Gibbs energy (GE) is negatively correlated with refrigerant solubility and COP.
作为一种环境可持续制冷技术,吸收式制冷循环(ARC)越来越受到人们的关注,研究新型工作对的汽液平衡(VLE)对推广和发展吸收式制冷循环具有重要意义。本文创新性地测量了烃类制冷剂R1270在303.15 K ~ 353.15 K的有机溶剂DMETrEG和NMP中的VLE,并采用非随机双液(NRTL)模型对实验数据进行拟合,评价其在ARC中的性能。R1270+DMETrEG的平均绝对相对偏差(AARD)和最大绝对相对偏差(MARD)分别为0.8318 %和2.8169%。对于R1270+NMP,对应的值为1.4472%和3.1676%。在ARC的热力学分析中,R1270+DMETrEG工作副的最大性能系数(COP)为0.327,高于R1270+NMP的0.313。与使用UNIFAC-DMD模型预测的COP值相比,从实验VLE数据计算的COP值大约低0.02-0.08,尽管总体趋势保持一致。在所有情况下,R1270+DMETrEG的COP均高于R1270+NMP,无论是实验结果还是预测结果。实验数据表明,R1270工作对的COP比R1234yf和R1234ze(E)低0.01 ~ 0.1。在恒定的发电温度下,这两个工作对表现出相似的COP趋势,作为蒸发和冷凝温度的函数。过量特性分析表明,过量吉布斯能(GE)与制冷剂溶解度和COP呈负相关。
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引用次数: 0
Study on the effect of air contamination in helium on the reliability of a Helium Joule-Thomson cryocooler 氦气中空气污染对氦焦耳-汤姆逊制冷机可靠性影响的研究
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-20 DOI: 10.1016/j.ijrefrig.2026.01.025
Pengcheng Qu , Zhichao Chen , Ziqi Wei , Yuanting Zhang , Jinjian Chu , Baoyu Yang , Xi Chen , Shaoshuai Liu
Gas contamination significantly limits the reliability of Helium Joule-Thomson Cryocoolers (JTC) in long-life space missions. Distinct from previous studies that focus on contamination during cooldown, this research investigates the dynamic clogging mechanism caused by gradual contaminant accumulation during continuous operation. A method of multiple quantitative additions of air is employed to explore the impact on operating characteristics. Experimental results reveal that air contaminants primarily desublimate into solid frost layers in the second-stage counter-flow heat exchanger (CFHX 2) within the temperature range of 65–15 K, eventually leading to clogging. This process exhibits four novel stages: nucleation-growth, steady-growth, collapse-reconstruction, and clogging. To elucidate the mechanism, a gas contaminant desublimation model is established. Simulation results confirm that nitrogen deposits heavily around 35–45 K within CFHX 2, serving as the primary source of flow resistance. Furthermore, the study uncovers that the synergistic deposition of multi-component contaminants may lead to the frost layer instability observed during the collapse-reconstruction stage. This work clarifies the dynamic clogging mechanism of air contamination, providing a quantitative basis for reliability assessment and suppression strategies for Helium JTCs.
气体污染严重限制了氦焦耳-汤姆逊制冷机(JTC)在长寿命太空任务中的可靠性。与以往研究集中于冷却过程中的污染不同,本研究研究的是连续运行过程中污染物逐渐积累所导致的动态堵塞机制。采用多次定量添加空气的方法来探讨对操作特性的影响。实验结果表明,在65 ~ 15 K的温度范围内,空气污染物主要在二级逆流换热器(CFHX 2)中升华为固体霜层,最终导致堵塞。这一过程表现出四个新的阶段:成核生长、稳定生长、坍缩重建和堵塞。为了阐明这一机理,建立了气体污染物再升华模型。模拟结果证实,在cfhx2中,氮气在35-45 K附近大量沉积,是流动阻力的主要来源。此外,研究还揭示了多组分污染物的协同沉降可能导致在塌陷-重建阶段观测到的霜层失稳。阐明了空气污染的动态堵塞机理,为氦jtc的可靠性评估和抑制策略提供了定量依据。
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引用次数: 0
Force fields for R1336mzz(Z), R1336mzz(E), R1233zd(E) and solvation in pentaerythritol tetrapentanoate (PEC5) lubricant R1336mzz(Z)、R1336mzz(E)、R1233zd(E)在四戊酸季戊四醇(PEC5)润滑油中的溶剂化力场
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-20 DOI: 10.1016/j.ijrefrig.2026.01.026
Ali Aminian
Low global warming potential refrigerants are playing major role regarding challenges that face the safety of environment. In this work, force field parameters were developed for cis-1,1,1,4,4,4-hexafluoro-2-butene (R1336mzz(Z)), trans-1,1,1,4,4,4-hexafluoro-2-butene (R1336mzz(E)), and trans-1‑chloro-3,3,3-trifluoro-1-propene (R1233zd(E)) based on high-level DFT calculations. The model parameters were tested to predict thermal conductivity, liquid density, dipole moment, and dielectric constant of the refrigerants, which were further compared against reference data. Molecular interaction studies showed that no strong interaction is exist between R1233zd(E) and pentaerythritol tetrapentanoate (PEC5). Also, the DFT calculations of the optimized structures were used for COSMO sigma-profile generations and solubility or vapor-liquid equilibrium (VLE) predictions.
低全球变暖潜能值制冷剂在应对环境安全挑战方面发挥着重要作用。在这项工作中,基于高阶DFT计算,建立了顺式-1,1,1,4,4,4-六氟-2-丁烯(R1336mzz(Z)),反式-1,1,1,4,4,4-六氟-2-丁烯(R1336mzz(E))和反式-1氯-3,3,3-三氟-1-丙烯(R1233zd(E))的力场参数。对模型参数进行了测试,以预测制冷剂的导热系数、液体密度、偶极矩和介电常数,并与参考数据进行了比较。分子相互作用研究表明,R1233zd(E)与季戊四醇四戊酸酯(PEC5)不存在强相互作用。此外,优化结构的DFT计算用于COSMO sigma-剖面生成和溶解度或汽液平衡(VLE)预测。
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引用次数: 0
The thermal insulation performance of variable metal coating multilayer insulation materials 变金属涂层多层保温材料的保温性能
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-18 DOI: 10.1016/j.ijrefrig.2026.01.024
Wenjie Zhou , Chenhui Jiang
Multi-layer insulation (MLI), recognized for its superior thermal performance, is commonly known as 'super insulation'. Traditional MLI designing utilizes one single metallic coating as the reflective layer, neglecting the impact of temperature variations on metal emissivity. This paper proposes a novel approach, variable metal-coating multilayer insulation (VM-MLI), employing aluminum, gold, and copper as reflective layer materials. By adopting an enhanced layer-by-layer model, theoretical analysis of VM-MLI is conducted within the temperature range of 20–300 K, comparing its thermal insulation performance to that of conventional MLI under the same conditions. Additionally, this study examines the influence of layer density, area ratio f, vacuum pressure, and hot boundary temperature on VM-MLI's heat leakage. The results reveal significant reduction in total heat leakage with VM-MLI. Under the conditions investigated, the heat leakage of MLI is 0.6027W/m2, while that of VM-MLI is 0.3936 W/m2 This represents a reduction in total heat leakage of 0.2091 W/m2,demonstrating approximately 35% better thermal insulation performance than that of the conventional MLI.
多层绝热材料(MLI)因其优越的热性能而被公认,通常被称为“超级绝热材料”。传统的MLI设计采用单一金属涂层作为反射层,忽略了温度变化对金属发射率的影响。本文提出了一种采用铝、金、铜作为反射层材料的可变金属涂层多层绝缘(VM-MLI)方法。采用增强的逐层模型,在20-300 K温度范围内对VM-MLI进行了理论分析,比较了相同条件下VM-MLI与常规MLI的保温性能。此外,本研究还考察了层密度、面积比f、真空压力和热边界温度对VM-MLI热泄漏的影响。结果表明,VM-MLI显著减少了总热泄漏。在研究条件下,MLI的热泄漏量为0.6027W/m2, VM-MLI的热泄漏量为0.3936 W/m2,总热泄漏量减少了0.2091 W/m2,保温性能比传统MLI提高了约35%。
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引用次数: 0
Performance study of a new dual condenser heat pump driven humidification and dehumidification desalination system 新型双冷凝器热泵加湿除湿海水淡化系统性能研究
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-15 DOI: 10.1016/j.ijrefrig.2026.01.021
Wenjie Yang , Yuefen Gao
Conventional two-stage humidification and dehumidification desalination systems usually use a sub-cooler as the initial heating source, limiting the heating capacity. Thus, this paper uses ejectors to propose a dual condenser heat pump driven humidification-dehumidification desalination (DCHP-HDH) system. The system uses dual condensers as a drive heat source to indirectly increase the heating and humidifying capacity of the humidifier. Makes the humid air coming out of the humidifier have a higher humidity ratio, and dual dehumidifiers are used to fully process this high-humidity ratio humid air. The performance of the system in this paper was evaluated using thermodynamic and economic analyses. The simulation results show that the DCHP-HDH system is capable of obtaining a maximum Gained Output Ratio (GOR) of 9.234, a maximum freshwater production of 49.88 kg/h, and a maximum Recovery Ratio (RR) of 5.542%, at which time the system's freshwater production cost is 0.0127$/L. In addition, higher evaporation temperatures and lower input seawater temperatures result in systems with better energy efficiency and freshwater production performance.
传统的两级加湿和除湿海水淡化系统通常使用一个分冷器作为初始热源,限制了加热能力。因此,本文采用喷射器提出了一种双冷凝器热泵驱动的加湿-除湿海水淡化系统。系统采用双冷凝器作为驱动热源,间接增加加湿器的加热加湿能力。使从加湿器出来的湿空气具有更高的湿度比,采用双除湿机对这种高湿度比的湿空气进行充分处理。本文对系统的性能进行了热力学分析和经济分析。仿真结果表明,DCHP-HDH系统的最大产出比(GOR)为9.234,最大淡水产量为49.88 kg/h,最大采收率为5.542%,此时系统的淡水生产成本为0.0127美元/L。此外,较高的蒸发温度和较低的输入海水温度使系统具有更好的能源效率和淡水生产性能。
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引用次数: 0
Experimental investigation on adiabatic two-phase frictional pressure drop of R290, R600a, and their mixtures in a horizontal minichannel R290、R600a及其混合物在水平小通道内绝热两相摩擦压降的实验研究
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-15 DOI: 10.1016/j.ijrefrig.2026.01.022
Na Liu, Hao Ji, Yeyi Liu, Run Li, Dezheng Sun, Qian Zhao, Jianxiang Guo
To support the application of eco-friendly refrigerants, this study reports the adiabatic two-phase frictional pressure drop of R290, R600a, and their mixtures (mass fractions 70/30, 50/50, 30/70) in a 1.54 mm horizontal minichannel. Experiments were conducted under specific operating conditions: mass fluxes of 200-400 kg/(m2·s), saturation temperatures of 22-26°C, and vapor quality ranging from 0 to 1.0. Key influencing factors were systematically analyzed, and results were compared with fluorinated refrigerants (R32, R1234ze(E), their mixtures) and existing predictive correlations. The results indicate that the frictional pressure drop is significantly influenced by mass flux, vapor quality, and R600a mass fraction. An increase in these parameters leads to a substantial rise in pressure drop, attributed to intensified gas-liquid interfacial shear stress and the thermophysical properties of R600a (low liquid-vapor density and high liquid viscosity). Within the narrow investigated temperature span (22-26°C), saturation temperature has a negligible impact, as the variation in reduced pressure is insufficient to cause significant shifts in thermophysical properties. While R290/R600a mixtures exhibit higher pressure drop than fluorinated refrigerants, they offer superior environmental performance (zero ozone depletion potential, ultra-low global warming potential). The Tran et al. correlation shows the highest predictive accuracy, with a mean absolute relative deviation of 10.75%, mean relative deviation of 6.15%, and 92.06% of data within ±30% deviation. This work enriches the pressure drop database for hydrocarbon mixtures in minichannels. It provides a reliable design tool and lays a foundation for optimizing compact heat exchangers using eco-friendly mixed refrigerants, especially in environmentally sensitive scenarios.
为了支持环保制冷剂的应用,本研究报告了R290、R600a及其混合物(质量分数为70/ 30,50 / 50,30 /70)在1.54 mm水平小通道中的绝热两相摩擦压降。实验在质量通量200 ~ 400 kg/(m2·s)、饱和温度22 ~ 26℃、蒸汽质量0 ~ 1.0的特定工况下进行。系统分析了关键影响因素,并与含氟制冷剂(R32、R1234ze(E)及其混合物)及已有预测相关性进行了比较。结果表明,质量流量、蒸汽质量和R600a质量分数对摩擦压降有显著影响。这些参数的增加导致压降大幅上升,这是由于气液界面剪切应力的增强以及R600a的热物理性质(低液-气密度和高液体粘度)。在狭窄的研究温度范围内(22-26°C),饱和温度的影响可以忽略不计,因为减压的变化不足以引起热物理性质的显著变化。虽然R290/R600a混合物比含氟制冷剂具有更高的压降,但它们具有优越的环保性能(零臭氧消耗潜势,超低全球变暖潜势)。Tran等人的相关性显示出最高的预测精度,平均绝对相对偏差为10.75%,平均相对偏差为6.15%,偏差在±30%以内的数据为92.06%。这项工作丰富了小通道烃类混合物的压降数据库。它提供了一个可靠的设计工具,并为使用环保混合制冷剂优化紧凑型热交换器奠定了基础,特别是在环境敏感的情况下。
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引用次数: 0
Influence of structural deformation on the rotordynamic characteristics of shaft labyrinth seals in cryogenic liquid turbine expanders under thermal-fluid-solid coupling 热-流-固耦合下结构变形对低温液体涡轮膨胀机轴篦密封转子动力特性的影响
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-15 DOI: 10.1016/j.ijrefrig.2026.01.023
Wanyou Sun, Jinju Sun, Peng Song, Shengyuan Wang
The study is focused on the rotordynamic characteristics of shaft labyrinth seals in cryogenic liquid turbine expanders. The critical operational conditions (-170 °C, 6–8 MPa) for cryogenic liquid turbine expanders have made its shaft labyrinth seal rotordynamic characteristics a thermal-fluid-solid coupled problem. The previous study performed by the team of authors indicates that seal structural deformation in cryogenic environments may not be negligible, whereas it calls for an in-depth study to identify their influence on the shaft labyrinth seal rotordynamic characteristics. This study develops a computational framework that combines the thermal-fluid-solid coupling approach and the whirling rotor method to predict the rotordynamic characteristics of labyrinth seals in cryogenic environments. This framework incorporates the influence of the seal structural deformation on the rotordynamic characteristics. It also circumvents the need for time-consuming transient analysis associated with rotor whirl. Consequently, it can enhance the reliability and efficiency of predicting the rotordynamic characteristics of labyrinth seals. In the present study, this framework is applied to investigate how multi-physics coupling-driven seal structural deformation affects rotordynamic characteristics of labyrinth seals for cryogenic liquid turbine expanders. Results demonstrate that multi-physics coupling-driven seal structural deformation alters the seal clearance profile, leading to significant changes in leakage flow behaviors, seal forces, rotordynamic coefficients, and seal stability. This primarily proves that the cryogenic structural deformation is an influential factor for the rotordynamic characteristics of labyrinth seals. Therefore, accounting for structural deformation is essential in the design and analysis of labyrinth seals for cryogenic liquid turbine expanders.
对低温液体涡轮膨胀机轴篦密封的转子动力学特性进行了研究。低温液体涡轮膨胀机的临界工况(-170℃,6-8 MPa)使其轴迷宫密封转子动力学特性成为一个热-流-固耦合问题。作者团队之前的研究表明,低温环境下密封结构变形可能不可忽略,但需要深入研究其对轴迷宫密封转子动力学特性的影响。本文提出了一种结合热-流-固耦合方法和旋转转子方法的计算框架,用于预测低温环境下迷宫密封的旋转动力学特性。该框架考虑了密封结构变形对转子动力特性的影响。它还避免了与转子旋转相关的耗时的瞬态分析的需要。从而提高了迷宫密封转子动力特性预测的可靠性和效率。在本研究中,应用该框架研究了多物理场耦合驱动的密封结构变形对低温液体涡轮膨胀机迷宫式密封转子动力学特性的影响。结果表明,多物理场耦合驱动的密封结构变形改变了密封间隙分布,导致泄漏流动特性、密封力、转子动力系数和密封稳定性发生显著变化。这初步证明了低温结构变形是影响迷宫式密封转子动力特性的一个因素。因此,在低温液体涡轮膨胀机迷宫密封的设计和分析中,考虑结构变形是必不可少的。
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引用次数: 0
Corrigendum to “Thermodynamic analysis of the refrigeration cycle of a domestic freezer with an electronic expansion valve” [International Journal of Refrigeration 181 (2026) 413–428] “带有电子膨胀阀的家用冷冻机制冷循环的热力学分析”的勘误表[国际制冷杂志181 (2026)413-428]
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-14 DOI: 10.1016/j.ijrefrig.2026.01.019
Kevin Wimmer, Jan Kummer, Michael Lang, Raimund Almbauer
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引用次数: 0
Experimental analysis of freezing times for fish and dummy loads in a horizontal CO₂ plate freezer at -50 °C evaporation temperature 在-50°C蒸发温度下,水平CO₂板冷冻机中鱼和虚拟负载冷冻时间的实验分析
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-13 DOI: 10.1016/j.ijrefrig.2026.01.017
Shuai Ren , Patrick Hadamitzky , Armin Hafner , Kristina Norne Widell
This study experimentally evaluated the performance of an industrial-scale horizontal CO₂ plate freezer integrated into an existing CO₂ refrigeration system. The shared central CO2 system can provide cooling capacity of 100 kW at evaporation temperature between -30 °C and -50 °C. Apple juice cartons were employed as the primary test material acting as dummy loads; freezing times were also measured for real fish samples—including mackerel, herring, and salmon. Freezing trials with apple juice cartons showed that lowering the average evaporation temperature from –36.1 °C to –48.6 °C reduced the average freezing time by 35.3%. Although the Coefficient of Performance (COP) was lower at reduced evaporation temperatures, specific energy consumption remained largely unaffected because of shorter freezing times. Additionally, lower circulation rates were associated with longer freezing time and higher specific energy consumption. The experimental results also demonstrated the importance of uniform plate contact, packaging material and configuration, and optimized product void space for freezing efficiency. A dynamic numerical model was developed and validated against the experimental data, showing good agreement with observed temperature profiles and freezing times.
本研究通过实验评估了将工业规模的卧式二氧化碳板冷冻机集成到现有的二氧化碳制冷系统中的性能。在-30℃~ -50℃蒸发温度范围内,共享中央CO2系统可提供100 kW的制冷量。以苹果汁纸盒为主要试验材料,作为虚拟载荷;他们还测量了真实鱼类样本的冷冻时间,包括鲭鱼、鲱鱼和鲑鱼。苹果汁纸箱的冷冻试验表明,将平均蒸发温度从-36.1°C降低到-48.6°C,平均冷冻时间缩短了35.3%。虽然在降低蒸发温度时性能系数(COP)较低,但由于冻结时间较短,比能量消耗在很大程度上没有受到影响。此外,较低的循环速率与较长的冷冻时间和较高的比能量消耗有关。实验结果还证明了均匀的板接触、包装材料和结构以及优化产品空隙空间对冷冻效率的重要性。建立了一个动态数值模型,并与实验数据进行了验证,该模型与观测到的温度分布和冻结时间吻合良好。
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引用次数: 0
Effect of cooling rate on the quality of biological samples during cryopreservation 低温保存过程中冷却速率对生物样品质量的影响
IF 3.8 2区 工程技术 Q1 ENGINEERING, MECHANICAL Pub Date : 2026-01-13 DOI: 10.1016/j.ijrefrig.2026.01.020
Shilin Pan, Ankuo Zhang
Cryopreservation, a fundamental technology in modern biomedicine, has driven substantial advancements across several pioneering fields, including cell therapy, reproductive medicine, and tissue engineering. In low-temperature environments, the rate of biochemical reactions in biological samples is significantly reduced, thereby prolonging their storage longevity. However, the essence of cryopreservation extends beyond merely maintaining low temperatures; the cooling rate emerges as a pivotal parameter during the cooling process, from ambient to storage temperatures, significantly influencing both the survival rate and efficacy of preservation. Furthermore, distinct biological samples exhibit varying sensitivities to cooling rates, with both excessively rapid and excessively slow cooling potentially inducing irreversible damage. Consequently, a comprehensive investigation and determination of the optimal cooling rates for different biological samples is essential to enhance the outcomes of cryopreservation. This review thoroughly examines the mechanisms by which cooling rates affect various biological samples, compares preservation outcomes across different cooling conditions with existing literature, and consolidates the optimal cooling rates for these samples. Research indicates that the optimal cooling rate for cell-like samples is generally below 10 °C/min whereas tissue-like samples typically require a slower rate, usually maintained below 1 °C/min. This controlled cooling process can be precisely achieved using a controlled-rate cooling device. Furthermore, this manuscript provides a brief overview of controlled-rate cooling techniques and the principles of slow freezing, offering a theoretical foundation for optimizing cryopreservation protocols for biological samples.
冷冻保存是现代生物医学的一项基础技术,在细胞治疗、生殖医学和组织工程等多个前沿领域取得了重大进展。在低温环境下,生物样品的生化反应速率显著降低,从而延长了生物样品的保存寿命。然而,低温保存的本质不仅仅是保持低温;在冷却过程中,从环境温度到储存温度,冷却速率是一个关键参数,对保鲜期和保鲜期都有重要影响。此外,不同的生物样品对冷却速率表现出不同的敏感性,过快和过慢的冷却都可能引起不可逆的损伤。因此,全面调查和确定不同生物样品的最佳冷却速率对于提高冷冻保存的结果至关重要。这篇综述深入研究了冷却速率影响各种生物样品的机制,与现有文献比较了不同冷却条件下的保存结果,并总结了这些样品的最佳冷却速率。研究表明,细胞样样品的最佳冷却速率通常低于10°C/min,而组织样样品通常需要较慢的冷却速率,通常保持在1°C/min以下。这种控制的冷却过程可以精确地实现使用控制速率冷却装置。此外,本文还简要概述了控制速率冷却技术和慢速冷冻原理,为优化生物样品的冷冻保存方案提供了理论基础。
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引用次数: 0
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International Journal of Refrigeration-revue Internationale Du Froid
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