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Assessment of Temperature and Metallic Stresses on a Psychrotolerant Bacterium Pseudomonas veronii ASP36 Isolated from Himalayan Region in Uttarakhand State, India 印度北阿坎德邦喜玛拉雅地区耐寒细菌维罗尼假单胞菌ASP36的温度和金属胁迫评价
Pub Date : 2016-03-01 DOI: 10.12983/IJSRES-2016-P0063-0070
A. S. Panwar, G. K. Joshi
Bacteria are one of the most versatile living creatures having ability to sustain life processes under various suboptimal conditions. Present work illustrates the impact of temperature and metallic stresses on a psychrotolerant bacterium isolated from Himalayan region in India. The bacterium was morphologically and biochemically characterized and identified as Pseudomonas veronii ASP36 based on the 16S rDNA sequencing followed by BLAST similarity search. Growth at low temperature incited the bacterium to produce more exopolysaccharides. Preincubation of the bacterium at near zero temperature enhanced its freeze tolerance. ZnSO4 induced genomic changes in the test isolate were revealed through RAPD.
细菌是最多才多艺的生物之一,有能力在各种次优条件下维持生命过程。目前的工作说明了温度和金属应力对从印度喜马拉雅地区分离的耐寒细菌的影响。通过16S rDNA测序和BLAST相似性搜索,鉴定该细菌为veronii假单胞菌ASP36。低温下的生长刺激细菌产生更多的胞外多糖。在接近零度的温度下进行预孵育,增强了细菌的抗冻能力。RAPD分析了ZnSO4诱导的基因组变化。
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引用次数: 0
Microbial Causes of Art Damage and Their Enzyme Profiles 艺术损伤的微生物成因及其酶谱
Pub Date : 2016-03-01 DOI: 10.12983/IJSRES-2016-P0078-0085
N. Rahman, W. Suen, S. Yip, New Jen Yan, Khoo Lay Pheck, N. See, Charmaine Ann, Celine Lloyd
A study was conducted to assess the enzyme profiles of microorganisms isolated from biologically damaged art- work. A total of 15 paintings were screened for fungi and bacteria using standard microbiological methods. Aspergillus spp (26.2%) and Bacillus spp (21.4%) were the most common causes of spoilage among 42 isolates. Representative fungal and bacterial isolates (n=14) were screened for 20 enzymes. All isolates (100%) possessed cellulolytic and phosphate mobilizing enzymes; 79% produced lipid-degrading enzymes. In the light of the changing industrial demand for the use of enzyme-based processes and non-animal enzyme sources, these microbial populations have a promising role.
我们进行了一项研究,以评估从生物损坏的艺术品中分离的微生物的酶谱。使用标准微生物学方法对总共15幅画作进行了真菌和细菌筛选。42株菌株中最常见的腐坏原因是曲霉(26.2%)和芽孢杆菌(21.4%)。有代表性的真菌和细菌分离株(n=14)筛选了20种酶。所有分离株(100%)均具有纤维素水解酶和磷酸动员酶;79%产生脂质降解酶。鉴于不断变化的工业对酶基工艺和非动物酶源的需求,这些微生物种群具有很好的作用。
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引用次数: 1
Investigation of Inorganic Complexes of Amidoamines Synthesized from Synthetic Oxy- and Petroleum Acids as Corrosion Inhibitor 以合成氧和石油酸为缓蚀剂合成氨基胺无机配合物的研究
Pub Date : 2016-02-01 DOI: 10.12983/IJSRES-2016-P0040-0046
L. Afandiyeva, V. Abbasov, S. Ahmadbayova, S. Hajiyeva, E. Azizbeyli, F. Tahirova, S. Aliyeva
Amidoamines have been synthesized based on the mixture of synthetic petroleum and oxy acids (SPA + OSPA) and polyethylene polyamine (PEPA). Physical and chemical indices of amidoamines have been identified. Complexes have been prepared from the amidoamine and HCl (Example 1), H3PO4 (Example 2), HNO3 (Example 3) in ratio 1:1. The kinetic effect of steel corrosion in 1% NaCl solution saturated with CO2 has been studied in ACM Gill AC potentiometer, at 50°C. Investigation was conducted at 25 and 50 ppm of each complex. It was found that, in Example 1 at 25 ppm after 20 hours of research the protection effect was 97.5%, at 50 ppm 97.3%. In Example 2 at 25 and 50 ppm corrosion protection were 92% and 90.4% after 20 hours of research. In Example 3 at 25 and 50 ppm after 20 hours of research protective effects were 97.5 and 97.3%. It was determined that, amidoamine derivatives obtained from SPA + OSPA and PEPA, at 25 ppm was the best result-98.3%. By Calculated Gibbs free energy and the image of the surface of used electrode it has been proved that, a complex in different concentrates easily was exposed to chemisorption on metal surface. Therefore protective film formed on the surface was stable.
以合成石油和氧酸(SPA + OSPA)和聚乙烯多胺(PEPA)为原料合成了偕胺类化合物。确定了偕胺类化合物的理化指标。用胺胺与盐酸(例1)、盐酸(例2)、硝酸(例3)按1:1的比例制备了配合物。在ACM Gill交流电位器上,研究了钢在50℃下,在饱和CO2的1% NaCl溶液中腐蚀的动力学效应。在每种复合物的25和50 ppm下进行了调查。研究发现,在25 ppm下,例1的防护效果为97.5%,在50 ppm下,防护效果为97.3%。在例2中,在25 ppm和50 ppm条件下,经过20小时的研究,防腐效果分别为92%和90.4%。在例3中,研究20小时后,在25和50 ppm下,保护效果为97.5%和97.3%。结果表明,SPA + OSPA和PEPA的偕胺胺衍生物在25 ppm时收率最高,为98.3%。通过计算的吉布斯自由能和电极表面图像,证明了不同浓度的配合物在金属表面容易发生化学吸附。因此在表面形成的保护膜是稳定的。
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引用次数: 0
Selective Harvesting Impact on Natural Regeneration, Tree Species Richness and Diversity in Forest Co-management Block in Liwonde Forest Reserve, Malawi 马拉维Liwonde森林保护区森林共管区选择性采伐对自然更新、树种丰富度和多样性的影响
Pub Date : 2016-02-01 DOI: 10.12983/IJSRES-2016-P0047-0054
Francis Kamangadazi, L. Mwabumba, Edward Missanjo, F. Phiri
Biodiversity conservation within forests depends, in part, on management practices that restore or maintain plant community diversity and function. Because many plant communities are adapted to natural disturbances, selective harvesting has potential to meet this need. This study was conducted to determine the impact of selective harvesting on tree species regeneration abundance, richness and diversity in Chidenga forest co-management block in Liwonde forest reserve, Malawi after four years of harvesting. The study constituted two treatments: harvested area (selective harvesting) and non-harvested area (control). The data obtained was analysed using Rẻnyi diversity profile in Biodiversity R. The results show that there were substantial variations between the treatments on the studied variables. Harvested area had higher tree species regenerants abundance (6088±452 ha -1 ) than non-harvested area (5324±364 ha -1 ). Harvested area had a higher Hα (2.64) at 0-alpha than Hα (2.48) for non-harvested area, indicating that harvested area had the higher tree species richness than non-harvested area in the understory. Furthermore, in the understory, harvested area had higher profile, 2.64 to 0.33 from 0-Alpha to infinity, than non- harvested area, 2.48 to 0.21 from 0-Alpha to infinity. This indicates that harvested area was more diverse than non-harvested area. In the overstory, harvested area had individual tree species evenly distributed than in non-harvested area. This signifies a healthy or good ecosystem. Therefore, it can be suggested that selective harvesting has a significant role in conservation of the forest resource base, and may be promoted for sustainable management of forest co-management blocks.
森林内生物多样性的保护部分取决于恢复或维持植物群落多样性和功能的管理做法。由于许多植物群落适应自然干扰,选择性采收有可能满足这一需求。本研究在马拉维Liwonde森林保护区Chidenga森林共管区块进行了4年的采伐,以确定选择性采伐对树种更新丰度、丰富度和多样性的影响。研究分为两种处理:采收区(选择性采收)和非采收区(对照)。利用生物多样性研究中心(biological R.)的Rẻnyi多样性剖面对数据进行分析。结果表明,不同处理间在研究变量上存在较大差异。采伐区树种再生物质丰度(6088±452 ha -1)高于非采伐区(5324±364 ha -1)。在0 α值处,采伐区的Hα值(2.64)高于非采伐区的Hα值(2.48),表明采伐区林下物种丰富度高于非采伐区。在林下,从0 α到无限远,采伐面积的轮廓值为2.64 ~ 0.33,高于未采伐面积的2.48 ~ 0.21。这表明收获面积比未收获面积更多样化。在林下,采伐区单株树种分布均匀,而非采伐区单株树种分布均匀。这意味着一个健康或良好的生态系统。因此,可以认为选择性采伐对森林资源基础的保护具有重要作用,并可能促进森林共同经营区块的可持续经营。
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引用次数: 3
Bacteriological and Physicochemical Characteristics of Kaptai Lake Water in Terms of Public Health Significance Kaptai湖水的细菌学和理化特征及其对公共卫生的意义
Pub Date : 2016-02-01 DOI: 10.12983/IJSRES-2016-P0031-0039
R. Barua, S. Barua, Fatema Tuz-Zohora, R. Mutsuddi, M. Uddin, H. Hasegawa, I. Rahman
Kaptai Lake (KL), which is one of the largest artificial freshwater lakes of South-east Asia, is located in the Rangamati district of Bangladesh and also the major dwelling place of the indigenous ethnic minorities. The lake water is consumed for drinking and other domestic purposes by the population living in islands within the lake without any intermediate purifying process. In the current study, the bacteriological, physicochemical and metal characteristics of the lake water have been measured to investigate the lake water quality regarding the health concerns in comparison with the limits set by WHO, USEPA and Bangladesh EQS. The heterotrophic plate count for the lake water samples were between ~75 and ~244 cfu mL -1 and was in compliance with the USEPA limit of 500 cfu mL -1 . However, the coliform count was in the range of 40.6 to 14017, and was above the standard limits set for drinking use. All the water samples also showed positive presence of Enterococcus spp., Salmonella spp., Pseudomonas spp. and Vibrio spp. The physicochemical analysis confirms that the water temperature (26-27 C), pH (7.5-7.8), electrical conductivity (108-113 µS cm -1 ), total dissolved solids (53-55 ppm), total hardness (42-43 ppm), total alkalinity (11-11.5 ppm), chloride (0.9-1.8 ppm) and dissolved oxygen (4.1-4.8 ppm) were within the limits of the guideline values. The concentrations (mg L -1 ) of toxic metal-species, e.g., lead (4.025-5.128), cadmium (0.008-0.020) and nickel (0.199-0.288) were found to be higher than the recommended regulatory limits, while the contents of copper (0.099-0.301), manganese (0.179-0.291) and zinc (1.989-2.278) were within the acceptable range. The findings from our work indicate that the water of KL is unsuitable for drinking use in terms of bacteriological and metal characteristics and might pose a long-term health risk to its consumers.
Kaptai湖(KL)是东南亚最大的人工淡水湖之一,位于孟加拉国的Rangamati区,也是土著少数民族的主要居住地。居住在湖内岛屿上的居民将湖水用于饮用和其他家庭用途,无需经过任何中间净化过程。在目前的研究中,对湖水的细菌学、物理化学和金属特性进行了测量,以便与世卫组织、美国环境保护局和孟加拉国环境质量体系规定的限值进行比较,调查湖泊水质对健康的影响。湖水样的异养板计数在~75 ~ ~244 cfu mL -1之间,符合美国环保局500cfu mL -1的限值。然而,大肠菌群数量在40.6至14017之间,超过了饮用水的标准限值。所有水样均检出肠球菌、沙门氏菌、假单胞菌和弧菌,理化分析证实水温(26 ~ 27℃)、pH(7.5 ~ 7.8)、电导率(108 ~ 113µS cm -1)、总溶解固形物(53 ~ 55 ppm)、总硬度(42 ~ 43 ppm)、总碱度(11 ~ 11.5 ppm)、氯化物(0.9 ~ 1.8 ppm)和溶解氧(4.1 ~ 4.8 ppm)均在指标值范围内。铅(4.025-5.128)、镉(0.008-0.020)和镍(0.199-0.288)等有毒金属的浓度(mg L -1)均高于建议的监管限值,而铜(0.099-0.301)、锰(0.179-0.291)和锌(1.989-2.278)的含量均在可接受范围内。我们的研究结果表明,就细菌和金属特性而言,吉隆坡的水不适合饮用,可能对消费者构成长期健康风险。
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引用次数: 7
Air Quality Monitoring in Uyo Metropolis, Akwa Ibom State, Niger Delta Region of Nigeria 尼日利亚尼日尔三角洲地区阿夸伊博姆州Uyo大都市空气质量监测
Pub Date : 2016-02-01 DOI: 10.12983/IJSRES-2016-P0055-0062
G. Ebong, V. Mkpenie
Assessment of air quality in Uyo metropolis was carried out by monitoring levels of nitrogen (IV) oxide, sulphur (IV) oxide, hydrogen sulphide, carbon (II) oxide, ammonia, chlorine gas and suspended particulate matter at four major locations within the study area for one week. Results obtained indicated higher mean concentrations of CO, NH3 and Cl2 in the morning hours, NO2, H2S and SPM in the afternoon while, SO2 did not vary considerably between these periods. Levels of these air particulates were above their recommended standards by FEPA indicating their nuisance status. However, Air quality index classified NO2 and SO2 in the very poor class, CO in moderate class while NH3 and SPM were in the very good category. Correlation analyses showed that, NO2 and SO2 exhibited strong positive relationships with H2S, CO and Cl2. Hydrogen sulphide showed common source and strong correlation with CO, NH3 and Cl2. Carbon (II) oxide correlated positively and significantly with Cl2 and SPM while, chlorine indicated significant positive association with SPM. Principal component analysis indicated two (2) major factors with 91.39% of total variance. Factor 1 represented impacts of fuel combustion and vehicular emissions whereas; factor 2 represented influences of organic matter decomposition and petroleum processing.
通过监测研究区域内四个主要地点的氧化氮(IV)、氧化硫(IV)、硫化氢、氧化碳(II)、氨、氯气和悬浮颗粒物的水平,对尤约大都市的空气质量进行了为期一周的评估。结果表明,北京市CO、NH3和Cl2的平均浓度在上午较高,NO2、H2S和SPM的平均浓度在下午较高,而SO2的平均浓度在这两个时段之间变化不大。这些空气微粒的水平高于FEPA建议的标准,表明它们是有害的。空气质量指数将NO2和SO2划分为极差级,CO划分为中等级,NH3和SPM划分为极好级。相关分析表明,NO2、SO2与H2S、CO、Cl2呈显著正相关。硫化氢与CO、NH3、Cl2来源相同,相关性强。碳(II)氧化物与Cl2和SPM呈显著正相关,氯与SPM呈显著正相关。主成分分析显示2个主因子占总方差的91.39%。因子1表示燃料燃烧和车辆排放的影响,而;因子2代表有机质分解和石油加工的影响。
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引用次数: 9
Evaluation of Sequential Batch Reactor (SBR) Cycle Design to Observe the Advantages of Selector Phase Biology to Achieve Maximum Nutrient Removal 评价序批式反应器(SBR)循环设计,观察选择相生物学在最大限度去除营养物方面的优势
Pub Date : 2015-06-20 DOI: 10.12983/ijsres-2015-p0234-0238
Sandip Magdum, S. Varigala, Gauri Minde, J. Bornare, V. Kalyanraman
Pilot plant studies of sequential batch reactor (SBR) cycles revealed the role of selector phase biology (SPB) involved in COD removal, nitrification, denitrification and total phosphate removal. The phases namely anoxic, anaerobic and aerobic in single tank reactor design of SBR were showed effective biological nutrient removal (BNR) > 90% on average in 4 h designed cycle time with 12 h of hydraulic retention time (HRT). The phases were 2 h fill which includes 1 h simultaneous fill and aeration, 1 h settle and 1 h decant. This cycle design found effective to create selective environment for microbes to carry biological organic and nutrient removal reactions with improvement in sludge volume index (SVI).
序批式反应器(SBR)中试研究揭示了选择相生物(SPB)在COD去除、硝化、反硝化和总磷酸盐去除中的作用。SBR单槽反应器设计中,在设计循环时间为4 h,水力停留时间为12 h的条件下,厌氧、厌氧、好氧三个阶段的生物营养物去除率平均为90%。相为2 h填充,包括1 h同时填充和曝气,1 h沉淀和1 h滗析。该循环设计有效地为微生物创造了选择性环境,进行生物有机和营养物去除反应,提高了污泥体积指数(SVI)。
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引用次数: 4
Comparison of Two Analytical Methods for Measurement of Iron Concentration in Groundwater in Chandrapur District, Central India 印度中部钱德拉普尔地区地下水铁浓度测定的两种分析方法比较
Pub Date : 2015-04-01 DOI: 10.12983/IJSRES-2015-P0129-0136
R. Kamble
Water is an indispensible part of human life. Groundwater is contributing significantly as a source of drinking, cooking and domestic purposes. Groundwater contains different heavy metals and iron is one of the dominating one. Chronic exposure of iron through groundwater consumption above WHO drinking water standard of 0.3 mg/L has well understood. To ascertain the impacts of groundwater iron on human health its accurate measurement is an essential aspect. A research has been carried out to compare two analytical methods: ICP method (ICP-OES Dv 7000) and Aquaquant Iron Analysis Kit Merck, range 0.25-15 mg/L, Germany (visual method). Total 36 groundwater samples in Chandrapur district of Central India were assessed for post monsoon season of 2013 (October) for iron concentration by these two analytical methods. Findings of the study suggested that ICP method gives more accurate results in a wide range of iron concentration under field conditions as compared with Aquaquant Iron Analysis Kit. At 0.0-1.0 mg/L of Fe results obtained by both these method were in accordance with each other. At 1.0-2.0 mg/L of Fe, ICP gave slight increased in concentration as compared with test kit. However, at 5.0 mg/L of Fe by test kit, results obtained by ICP method was significantly lower.
水是人类生活不可缺少的一部分。地下水作为饮用、烹饪和家庭用途的来源发挥着重要作用。地下水中含有多种重金属,铁是主要的重金属之一。通过饮用超过世卫组织饮用水标准0.3毫克/升的地下水而长期接触铁已得到充分了解。确定地下水铁对人体健康的影响,其准确测量是一个重要方面。比较了两种分析方法:ICP法(ICP- oes Dv 7000)和Aquaquant Iron Analysis Kit Merck,范围0.25-15 mg/L,德国(目测法)。采用这两种分析方法对2013年季风季节后(10月)印度中部Chandrapur地区的36份地下水样品进行了铁浓度评估。研究结果表明,与Aquaquant iron Analysis Kit相比,ICP方法在现场条件下的铁浓度范围更广,结果更准确。在铁浓度为0.0 ~ 1.0 mg/L时,两种方法的测定结果基本一致。在1.0 ~ 2.0 mg/L Fe时,ICP浓度较试剂盒略有升高。而当检测试剂盒中Fe浓度为5.0 mg/L时,ICP法得到的结果明显较低。
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引用次数: 0
Pattern of Damage by Rodent (Rodentia: Muridae) Pests in Wheat in Conjunction with Their Comparative Densities throughout Growth Phase of Crop 小麦鼠类害虫危害模式及其在作物生育期的相对密度
Pub Date : 2015-04-01 DOI: 10.12983/IJSRES-2015-P0159-0166
M. Sarwar
Rodents are one of the most important vertebrate pests which directly and indirectly distress to the production of crops and storage of grains. Rodents especially under the Family Muridae can cause serious damage to cereal crops of all kinds including wheat. The present investigation was conducted to observe the pattern of damage by rodent (Rrodentia: Muridae) pests in wheat in conjunction with their comparative densities throughout growth phase of crop i.e., from seedling to mature grain stage. During the crop growing season, several rodent species were involved in damaging to wheat, but variations in their predominance were detected. Of the trapped and identified pests, the lesser bandicoot rat Bandicota bengalensis Gray was the mainly prime species in the wheat fields approached by Indian gerbil Tatera indica Hardwicke, house mouse Mus musculus L., soft-furred field rat Rattus meltada Gray and short tailed mole rat Nesokia indica Gray & Hardwicke. These different rodent pests began to raid and damage the wheat crop right from sowing and continued up to the time of harvesting. Such descriptions of damage are influenced by a number of factors, for instance, relative abundance of different growth stages of crop and their relative nutritional contents, palatability, tastes, and the availability of other food in the locality. The trends of different levels of damage through wheat stem cuttings at various growth stages served primarily as an indication that rats and mice are opportunistic foragers, adjusting their preferences according to availability of food resources. This information of food habits by specific rodents is a basis for the management of their populations and to reduce the damage in the wheat fields thereby increasing the grain yield.
啮齿动物是脊椎动物中最重要的害虫之一,直接或间接地危害着农作物的生产和粮食的储存。啮齿类动物,尤其是鼠科啮齿类动物,会对包括小麦在内的各种谷类作物造成严重损害。本研究旨在观察小麦各生育期(即从苗期到籽粒成熟期)鼠类害虫对小麦的危害规律及其相对密度。在作物生长季节,几种啮齿动物对小麦有危害,但其优势程度存在差异。在捕获和鉴定的害虫中,印度沙鼠(Tatera indica Hardwicke)、家鼠(musmusus L.)、软毛田鼠(Rattus meltada Gray)和短尾鼹鼠(Nesokia indica Gray & Hardwicke)接近麦田的主要害虫是小头土鼠(Bandicota bengalensis Gray)。这些不同的啮齿动物从播种开始就开始袭击和破坏小麦作物,并一直持续到收获季节。这种对损害的描述受到许多因素的影响,例如,作物不同生长阶段的相对丰度及其相对营养成分、适口性、口味以及当地其他食物的供应情况。小麦茎秆扦插在不同生长阶段造成不同程度伤害的趋势主要表明,大鼠和小鼠是机会主义觅食者,根据食物资源的可用性调整其偏好。这些特定啮齿动物的饮食习惯信息是管理其种群和减少对麦田的破坏从而提高粮食产量的基础。
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引用次数: 14
Evaluation of the Spatio-Temporal Variability in River and Groundwater Interactions, an Example from South West of Iran 河流与地下水相互作用的时空变异评价——以伊朗西南部为例
Pub Date : 2015-04-01 DOI: 10.12983/IJSRES-2015-P0147-0158
M. Chitsazan, M. Faryabi, A. Zarasvandi, A. Negarestani
River - groundwater interaction is one of the most common types of surface water and groundwater exchanges that occur in alluvial aquifers. These interactions are variable with space and time and received great attentions in recent years. In this research, the natural tracers such as 222 Rn, natural heat and common water quality characteristics were applied to evaluation of the river- groundwater interactions. The study area is Chamgolak area that is located in the northeast of the Andimeshk city in the south west of Iran. The Dez River is the major surface water body that interacts with groundwater in this area. The temporal and spatial variation of water exchanges between the river and groundwater were evaluated in this research. For this purpose, some common water quality parameters such 222 Rn, temperature, electrical conductivity (EC) and nitrate were measured in 2012- 2013 water year. The temporal variation patterns of common water quality parameters shows that the river recharge water influences the groundwater quality, so that the amounts of electrical conductivity (EC), temperature and nitrate show a notable decrease in river adjacent wells especially in wet period. "The spatial pattern of nitrate concentration shows that the topographic conditions of the Dez River channel also influence the rate of river - groundwater interactions, so that the maximum water exchanges between river and groundwater occur in the convex part of the Dez River meander near the Chamgolak town. This study show that the Dez River has a losing nature and recharges the groundwater but with rates that is vary temporally and spatially.
河流-地下水相互作用是发生在冲积含水层中最常见的地表水和地下水交换类型之一。这些相互作用随时间和空间的变化而变化,近年来受到广泛关注。本研究采用自然示踪剂如222rn、自然热和常见水质特征来评价河流-地下水相互作用。研究区域是位于伊朗西南部Andimeshk市东北部的Chamgolak地区。德兹河是该地区与地下水相互作用的主要地表水。本文对河流与地下水水量交换的时空变化进行了评价。为此,对2012- 2013水年的222 Rn、温度、电导率(EC)、硝酸盐等常见水质参数进行了测量。常见水质参数的时间变化规律表明,河流回灌对地下水水质有一定的影响,河流邻近井的电导率、温度和硝酸盐含量在湿润期明显下降。“硝酸盐浓度的空间格局表明,德兹河河道的地形条件也影响河流与地下水相互作用的速率,因此河流与地下水之间最大的水交换发生在靠近Chamgolak镇的德兹河曲流的凸起部分。”研究表明,德兹河具有流失的性质,对地下水进行补给,但补给速率随时间和空间的变化而变化。
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引用次数: 1
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International Journal of Scientific Research in Environmental Sciences
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