Pub Date : 2020-12-01DOI: 10.18052/www.scipress.com/ilns.80.51
C. Palanisamy, Bo Cui, H. Zhang, T. Nguyen, H. Tran, T. Khanh, Van Quan Nguyen, T. Xuan
(2E,6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-ol or farnesol is a natural isoprenoid possessing a range of beneficial biological activities as anti-candidiasis, anti-tumor and anti-hyperglycaemia. The present study, for the first time, isolated and identified farnesol from Euclea crispa leaves’ extract, subsequently, validated its antioxidant and antimicrobial potentials. The isolated compound was confirmed by spectroscopic techniques including ultraviolet-visible (UV/Vis), fourier-transform infrared (FTIR), and 1H and 13C magnetic resonance (NMR) spectroscopies. By in vitro investigations, farnesol exhibited a considerable antioxidant activity with IC50 values of 113.79, 109.59, and 116.65 µg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and nitric oxide radical scavenging assays, respectively. Additionally, farnesol performed a potent reducing power capacity which was in line with ascorbic acid, an outstanding antioxidant. By the disc-diffusion assay, farnesol exposed the superior antimicrobial activity against various disease-causing microorganisms. Significantly, at the concentration of 50 µg/mL, the compound effectively inhibited the growth of Escherichia coli and Aspergillus niger with inhibition zones of 12 and 11 mm, respectively. Findings from this research suggest that E. crispa leaf is a potential source of farnesol, a powerful antioxidant and antimicrobial agent.
{"title":"Characterization of (2E,6E)-3,7,11-Trimethyldodeca-2,6,10-Trien-1-Ol with Antioxidant and Antimicrobial Potentials from Euclea Crispa (Thunb.) Leaves","authors":"C. Palanisamy, Bo Cui, H. Zhang, T. Nguyen, H. Tran, T. Khanh, Van Quan Nguyen, T. Xuan","doi":"10.18052/www.scipress.com/ilns.80.51","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.80.51","url":null,"abstract":"(2E,6E)-3,7,11-Trimethyldodeca-2,6,10-trien-1-ol or farnesol is a natural isoprenoid possessing a range of beneficial biological activities as anti-candidiasis, anti-tumor and anti-hyperglycaemia. The present study, for the first time, isolated and identified farnesol from Euclea crispa leaves’ extract, subsequently, validated its antioxidant and antimicrobial potentials. The isolated compound was confirmed by spectroscopic techniques including ultraviolet-visible (UV/Vis), fourier-transform infrared (FTIR), and 1H and 13C magnetic resonance (NMR) spectroscopies. By in vitro investigations, farnesol exhibited a considerable antioxidant activity with IC50 values of 113.79, 109.59, and 116.65 µg/mL for 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl, and nitric oxide radical scavenging assays, respectively. Additionally, farnesol performed a potent reducing power capacity which was in line with ascorbic acid, an outstanding antioxidant. By the disc-diffusion assay, farnesol exposed the superior antimicrobial activity against various disease-causing microorganisms. Significantly, at the concentration of 50 µg/mL, the compound effectively inhibited the growth of Escherichia coli and Aspergillus niger with inhibition zones of 12 and 11 mm, respectively. Findings from this research suggest that E. crispa leaf is a potential source of farnesol, a powerful antioxidant and antimicrobial agent.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47973416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.18052/www.scipress.com/ilns.80.25
Shahbaz Khan, Syed Adnan Mazhar, Soahil Irshad, M. Nawaz, S. Bashir, M. Saddiq, Ali Bushkh
Optimum usage of fertilizers is an important factor that defines the fate of crop yield by confirming the accessibility of nutrients in soil. Imbalance use of fertilizer not only reduces the crop productivity but also adversely affects the soil properties. A one-year experiment was carried out to explore the impact of different levels of farm yard manure and urea on soil properties, growth and yield of wheat crop. Treatments were application of nitrogen at i) 125 kg ha-1 from urea, ii) 80 kg ha-1 of nitrogen from urea + 10 tons of farm yard manure ha-1 and iii) 20 tons of farm yard manure ha-1. Wheat cultivar Sehar-2006 was used in the experiment. All the treatments’ combination affected crop growth, economic yield and soil properties. However, maximum positive impact of combined use of farm yard manure and urea was observed. Results indicated that productive tillers per unit area (16%), plant height, number of spikelets per spike (12.5%) and economical yield (11%) were greatly enhanced by combined usage of urea and farm yard manure as compared to sole application of urea. Combined application of urea and farm yard manure also positively affected the soil bulk density, particle density, percent porosity and soil saturated hydraulic conductivity as compared to either sole use of urea or farm yard manure. Combined usage of farm yard manure and urea are suggested better to produce higher economical yield along with improved soil properties.
肥料的最佳使用是决定作物产量命运的一个重要因素，它确定了土壤中养分的可及性。施肥不平衡不仅会降低作物产量，而且会对土壤性质产生不利影响。通过为期一年的试验，探讨不同水平的农家肥和尿素对小麦土壤性质、生长和产量的影响。施氮量分别为:1)尿素氮125 kg ha-1, 2)尿素氮80 kg ha-1 +农家肥ha-1 10吨，3)农家肥ha-1 20吨。试验选用小麦品种Sehar-2006。所有处理的组合对作物生长、经济产量和土壤性质都有影响。而厩肥与尿素配施的正效应最大。结果表明，与单独施用尿素相比，尿素与厩肥配合施用可显著提高水稻单位面积有效分蘖数(16%)、株高、穗数(12.5%)和经济产量(11%)。与单独施用尿素或厩肥相比，尿素和厩肥配施对土壤容重、颗粒密度、孔隙率和土壤饱和导水率均有显著的正向影响。建议将农家肥与尿素混施，既能提高经济产量，又能改善土壤性状。
{"title":"Integrated Usage of Farm Yard Manure and Urea Improves Wheat Yield and Soil Properties","authors":"Shahbaz Khan, Syed Adnan Mazhar, Soahil Irshad, M. Nawaz, S. Bashir, M. Saddiq, Ali Bushkh","doi":"10.18052/www.scipress.com/ilns.80.25","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.80.25","url":null,"abstract":"Optimum usage of fertilizers is an important factor that defines the fate of crop yield by confirming the accessibility of nutrients in soil. Imbalance use of fertilizer not only reduces the crop productivity but also adversely affects the soil properties. A one-year experiment was carried out to explore the impact of different levels of farm yard manure and urea on soil properties, growth and yield of wheat crop. Treatments were application of nitrogen at i) 125 kg ha-1 from urea, ii) 80 kg ha-1 of nitrogen from urea + 10 tons of farm yard manure ha-1 and iii) 20 tons of farm yard manure ha-1. Wheat cultivar Sehar-2006 was used in the experiment. All the treatments’ combination affected crop growth, economic yield and soil properties. However, maximum positive impact of combined use of farm yard manure and urea was observed. Results indicated that productive tillers per unit area (16%), plant height, number of spikelets per spike (12.5%) and economical yield (11%) were greatly enhanced by combined usage of urea and farm yard manure as compared to sole application of urea. Combined application of urea and farm yard manure also positively affected the soil bulk density, particle density, percent porosity and soil saturated hydraulic conductivity as compared to either sole use of urea or farm yard manure. Combined usage of farm yard manure and urea are suggested better to produce higher economical yield along with improved soil properties.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48369616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-01DOI: 10.18052/www.scipress.com/ilns.80.43
Exur M. Buenaflor, R. Seronay, J. Jumawan
Size-structure analyses and gonad characteristics of the elongate sunset clam Gari elongata from the coastal waters of Hinatuan, Surigao del Sur, Philippines, was determined in this study. An opportunistic sampling approach was applied during the collection of G. elongata from the coastal waters of Brgy. Loyola and Brgy. Aquino. A total of 1437 G. elongata individuals were subjected to length-weight measurements, and gonads from five (5) individuals per sex among size classes were processed for histological characterization. The maximum total length of G. elongata recorded is 5.42 cm, way smaller than its congeners elsewhere, which is at 7.0-9.2 cm. The size-structure analysis shows that G. elongata from Brgy. Loyola exhibited negative allometric growth while those gleaned from Brgy. Aquino exhibited positive allometry indicating variation in growth dynamics. Known as a gleaning source, Barangay Loyola is abundant in G. elongata, but individuals are much smaller compared to those gathered from Barangay Aquino. A ratio of 1:1 for male and female classification was observed, as seen from gonad histological analyses. Ovaries were found in the spawning capable state with gonads in ripe (30%), spent (25%), early development (25%), and partially spawning (20%) stages. Testes were found to be in the ripe (40%), early developing (10%), spent (10%), and partially spawning (40%) stages. Gari elongata exhibits spawning capable gonads at 2.85 cm and 2.95 cm for males and females, respectively, which suggests that the shellfish has adapted early to reproduction to maintain its population in the coastal waters of Hinatuan. The small body lengths of individuals with mature gonads indicate that G. elongata may have been exposed to some stress in their natural environment. Regulated management of G. elongata to avoid over-harvesting and assessment of water and sediment quality of their natural habitat is highly recommended
本研究测定了来自菲律宾Surigao del Sur Hinatuan沿海水域的细长日落蛤Gari elongata的大小结构分析和性腺特征。在从布吉沿海水域采集龙舌兰的过程中，采用了机会性采样方法。Loyola和Brgy。阿基诺。共对1437个伸长G.elongata个体进行了长度-重量测量，并对来自不同性别的5个个体的性腺进行了组织学表征。龙舌兰的最大总长度为5.42厘米，远小于其他同类的7.0-9.2厘米。Loyola表现出负异速生长，而从Brgy收集到的则表现出负的异速生长。阿基诺表现出正的异速生长，表明生长动力学发生了变化。巴郎盖-洛约拉是一种已知的采集来源，在龙舌兰中有大量的，但与从巴郎盖阿基诺采集的个体相比，个体要小得多。从性腺组织学分析中可以看出，雄性和雌性的分类比例为1:1。卵巢处于产卵能力状态，性腺处于成熟（30%）、成熟（25%）、早期发育（25%）和部分产卵（20%）阶段。试验发现，睾丸处于成熟（40%）、早期发育（10%）、衰竭（10%）和部分产卵（40%）阶段。长盖石鳖的雄性和雌性性腺分别为2.85厘米和2.95厘米，具有产卵能力，这表明这种贝类很早就适应了繁殖，以维持其在Hinatuan沿海水域的种群数量。性腺成熟的个体的体长较小，这表明长柄G.elongata可能在其自然环境中受到了一些压力。强烈建议对龙舌兰进行规范管理，以避免过度捕捞，并评估其自然栖息地的水和沉积物质量
{"title":"Size Structure and Gonad Characterization of Gari elongata (Lamarck 1818) from the Coastal Areas of Hinatuan, Surigao Del Sur, Philippines","authors":"Exur M. Buenaflor, R. Seronay, J. Jumawan","doi":"10.18052/www.scipress.com/ilns.80.43","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.80.43","url":null,"abstract":"Size-structure analyses and gonad characteristics of the elongate sunset clam Gari elongata from the coastal waters of Hinatuan, Surigao del Sur, Philippines, was determined in this study. An opportunistic sampling approach was applied during the collection of G. elongata from the coastal waters of Brgy. Loyola and Brgy. Aquino. A total of 1437 G. elongata individuals were subjected to length-weight measurements, and gonads from five (5) individuals per sex among size classes were processed for histological characterization. The maximum total length of G. elongata recorded is 5.42 cm, way smaller than its congeners elsewhere, which is at 7.0-9.2 cm. The size-structure analysis shows that G. elongata from Brgy. Loyola exhibited negative allometric growth while those gleaned from Brgy. Aquino exhibited positive allometry indicating variation in growth dynamics. Known as a gleaning source, Barangay Loyola is abundant in G. elongata, but individuals are much smaller compared to those gathered from Barangay Aquino. A ratio of 1:1 for male and female classification was observed, as seen from gonad histological analyses. Ovaries were found in the spawning capable state with gonads in ripe (30%), spent (25%), early development (25%), and partially spawning (20%) stages. Testes were found to be in the ripe (40%), early developing (10%), spent (10%), and partially spawning (40%) stages. Gari elongata exhibits spawning capable gonads at 2.85 cm and 2.95 cm for males and females, respectively, which suggests that the shellfish has adapted early to reproduction to maintain its population in the coastal waters of Hinatuan. The small body lengths of individuals with mature gonads indicate that G. elongata may have been exposed to some stress in their natural environment. Regulated management of G. elongata to avoid over-harvesting and assessment of water and sediment quality of their natural habitat is highly recommended","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45323273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuliia Zhukova, N. Demchuk, N. Yorkina, Y. Dubinina, D. Ganzha, Liudmila Bezugla, T. Ilchenko
The role of spatial variables, soil properties and overstorey structure in spatial variation of the herb-layer community in a riparian mixed forest was shown. The research were conducted in the "Dnipro-Orils’kiy" Nature Reserve (Ukraine). The research polygon was laid in a forest in the floodplain of the River Protich, which is a left inflow of the River Dnipro. Plant abundance was quantified by measuring cover within experimental polygon. The experimental polygon consisted of 7 transects and each transect was made up of 15 test points. The distance between rows in the site was 3 m. At the site we established a plot of 45×21 m, with 105 subplots of 3×3 m organized in a regular grid. Vascular plant species lists were recorded for each 3×3 m subplot along with visual estimates of species cover projection. The plant community was represented by 43 species, of which 18.6% were phanerophytes, 39.5% were hemikriptophytes, 9.3% were therophytes, 7.0% were geophytes. An overall test of random labelling revealed the total nonrandom distribution of the tree stems within the site. Constrained correspondence analysis (CCA) was applied as ordination approach. The forward selection procedure allowed us to select 6 soil variables, which explain 28.3% of the herb-layer community variability. The list of the important soil variables includes soil mechanical impedance (at the depth 0–5, 30–35, 75–80, and 95–10 cm), soil moisture, and soil bulk density. The variation explained by pure spatial variables is equal to 11.0 %. The majority of the tree-distance structured variation in plant community composition was broad-scaled. The significant relationship was found between the pure spatial component of the community variation and a lot of phytoindicator estimations of which the variability of damping and humidity were of the greatest importance. Trees stand was demonstrated to be a considerable factor structuring both the herb-layer community and spatial variation of the physical properties of soil.
{"title":"The Small-Scale Variation of Herb-Layer Community Structure in a Riparian Mixed Forest","authors":"Yuliia Zhukova, N. Demchuk, N. Yorkina, Y. Dubinina, D. Ganzha, Liudmila Bezugla, T. Ilchenko","doi":"10.15421/012050","DOIUrl":"https://doi.org/10.15421/012050","url":null,"abstract":"The role of spatial variables, soil properties and overstorey structure in spatial variation of the herb-layer community in a riparian mixed forest was shown. The research were conducted in the \"Dnipro-Orils’kiy\" Nature Reserve (Ukraine). The research polygon was laid in a forest in the floodplain of the River Protich, which is a left inflow of the River Dnipro. Plant abundance was quantified by measuring cover within experimental polygon. The experimental polygon consisted of 7 transects and each transect was made up of 15 test points. The distance between rows in the site was 3 m. At the site we established a plot of 45×21 m, with 105 subplots of 3×3 m organized in a regular grid. Vascular plant species lists were recorded for each 3×3 m subplot along with visual estimates of species cover projection. The plant community was represented by 43 species, of which 18.6% were phanerophytes, 39.5% were hemikriptophytes, 9.3% were therophytes, 7.0% were geophytes. An overall test of random labelling revealed the total nonrandom distribution of the tree stems within the site. Constrained correspondence analysis (CCA) was applied as ordination approach. The forward selection procedure allowed us to select 6 soil variables, which explain 28.3% of the herb-layer community variability. The list of the important soil variables includes soil mechanical impedance (at the depth 0–5, 30–35, 75–80, and 95–10 cm), soil moisture, and soil bulk density. The variation explained by pure spatial variables is equal to 11.0 %. The majority of the tree-distance structured variation in plant community composition was broad-scaled. The significant relationship was found between the pure spatial component of the community variation and a lot of phytoindicator estimations of which the variability of damping and humidity were of the greatest importance. Trees stand was demonstrated to be a considerable factor structuring both the herb-layer community and spatial variation of the physical properties of soil.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42730946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.18052/www.scipress.com/ilns.79.9
W. Fernando, B. Karunarathna
Improved soil fertility is a requirement for enhanced crop production. Combination of inorganic and organic fertilizers improve crop productivity while reduce environmental degradation. An experiment was carried out at Eastern University, Sri Lanka to study the effect of Foliar Application of Banana Pseudostem Sap on Yield of Cowpea (Vigna unguiculata L. Walp.) in sandy regosol. The experiment was laid out in Randomized Complete Block Design having five treatments viz; recommended inorganic fertilizer N, P, K as basal and N as topdressing (T1), N, P, 1⁄2 K as basal with recommended N as topdressing and foliar spray of 1% , 3%, 5% and 7% banana pseudostem sap solution (T2-T5) at 3rd, 5th, 7th and 9th week after planting. The results revealed that significant difference (P<0.05) were noted on number of pods per plant at 1st and 2nd picking. Higher number of pods per plant, maximum pod length and girth were in T2 while low in T1. Sun dried weights of pods and number of seeds per pod were significantly (P<0.05) varied at each picking. Further, cowpea yield at each picking were higher in T2 compared to tested treatments. The present study suggested that, among the tested treatments N, P, 1⁄2 K as basal with recommended N as topdressing and foliar spray of 1% Pseudostem sap solution at 3rd, 5th, 7th and 9th WAP would be the most suitable for cowpea production in sandy regosol.
{"title":"Effect of Foliar Application of Banana Pseudostem Sap on Yield of Cowpea (Vigna unguiculata L. Walp.) \u0000 ","authors":"W. Fernando, B. Karunarathna","doi":"10.18052/www.scipress.com/ilns.79.9","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.79.9","url":null,"abstract":"Improved soil fertility is a requirement for enhanced crop production. Combination of inorganic and organic fertilizers improve crop productivity while reduce environmental degradation. An experiment was carried out at Eastern University, Sri Lanka to study the effect of Foliar Application of Banana Pseudostem Sap on Yield of Cowpea (Vigna unguiculata L. Walp.) in sandy regosol. The experiment was laid out in Randomized Complete Block Design having five treatments viz; recommended inorganic fertilizer N, P, K as basal and N as topdressing (T1), N, P, 1⁄2 K as basal with recommended N as topdressing and foliar spray of 1% , 3%, 5% and 7% banana pseudostem sap solution (T2-T5) at 3rd, 5th, 7th and 9th week after planting. The results revealed that significant difference (P<0.05) were noted on number of pods per plant at 1st and 2nd picking. Higher number of pods per plant, maximum pod length and girth were in T2 while low in T1. Sun dried weights of pods and number of seeds per pod were significantly (P<0.05) varied at each picking. Further, cowpea yield at each picking were higher in T2 compared to tested treatments. The present study suggested that, among the tested treatments N, P, 1⁄2 K as basal with recommended N as topdressing and foliar spray of 1% Pseudostem sap solution at 3rd, 5th, 7th and 9th WAP would be the most suitable for cowpea production in sandy regosol.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42002945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.18052/www.scipress.com/ilns.79.23
E. Adeyeye, O. Ayodele, J. Orege
Amino acids composition of Thryonomysswingerianus is reported. Whereas protein values (g100g-1) were liver (74.1), kidney (91.5), heart (84.6); corresponding total amino acid values were 93.5, 83.2 and 80.6. True protein from the crude protein of the samples ran thus: liver>kidney>heart. Of the twenty parameters reported on, liver was best in 12/20 (60.0%), kidney and heart both shared the second position of 4/20(20%) each. Among the essential amino acids, leucine predominated in both liver (7.96g100g-1 protein) and kidney (8.11g100g-1 protein) but valine (6.21g100g-1 protein) predominated in the heart. The P-PER values were; P-PER1: 2.78 (liver), 2.91(kidney), 0.716 (heart) and P-PER2: 2.71 (liver), 2.90 (kidney), 0.564 (heart). However, there was a reverse between liver and kidney in the EAAI values with liver (92.0) > kidney (90.2) > heart (87.6) with corresponding BV values of 88.5 > 86.6 > 83.7. In the amino acids scoring pattern, Ser was limiting in liver (0.533) and heart (0.394) but Thr (0.490) in kidney in whole hen’s egg score comparison; in FAO/WHO scoring standards, Thr was limiting in liver (0.988) and kidney (0.625) but Leu (0.459) in heart. In pre-school requirements, liver recorded no limiting amino acid whereas Thr was limiting in kidney (0.735) and Leu was limiting in the heart (0.486). T.swingerianus red viscera was compared with the red viscera of livestock animals (cattle, sheep and pork) as well as FAO/WHO/UNU standards for total essential amino acids. Our results when compared with the livestock red viscera (without Trp) and FAO/WHO/UNU (g100g-1 protein), we have heart: grasscutter/cattle/sheep/pig:45.3 /46.0/42.7/46.6; kidney: grasscutter/cattle/sheep/pig: 47.6/43.8/42.5/46.7; liver: grasscutter/cattle/sheep/pig: 50.7/47.7/41.5/47.5 and grasscutter liver/kidney/heart/ FAO/WHO/UNU:50.7/47.6/45.3/32.8 showing that all the red viscera values in T.swingerianus were better than the essential amino acids in the FAO/WHO/UNU standards and livestock red viscera. Statistical values showed that significant differences existed among the samples at r=0.01.
{"title":"Amino Acids Composition of Liver, Heart and Kidneys of Thryonomys swingerianus (Temminck 1827) Compared","authors":"E. Adeyeye, O. Ayodele, J. Orege","doi":"10.18052/www.scipress.com/ilns.79.23","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.79.23","url":null,"abstract":"Amino acids composition of Thryonomysswingerianus is reported. Whereas protein values (g100g-1) were liver (74.1), kidney (91.5), heart (84.6); corresponding total amino acid values were 93.5, 83.2 and 80.6. True protein from the crude protein of the samples ran thus: liver>kidney>heart. Of the twenty parameters reported on, liver was best in 12/20 (60.0%), kidney and heart both shared the second position of 4/20(20%) each. Among the essential amino acids, leucine predominated in both liver (7.96g100g-1 protein) and kidney (8.11g100g-1 protein) but valine (6.21g100g-1 protein) predominated in the heart. The P-PER values were; P-PER1: 2.78 (liver), 2.91(kidney), 0.716 (heart) and P-PER2: 2.71 (liver), 2.90 (kidney), 0.564 (heart). However, there was a reverse between liver and kidney in the EAAI values with liver (92.0) > kidney (90.2) > heart (87.6) with corresponding BV values of 88.5 > 86.6 > 83.7. In the amino acids scoring pattern, Ser was limiting in liver (0.533) and heart (0.394) but Thr (0.490) in kidney in whole hen’s egg score comparison; in FAO/WHO scoring standards, Thr was limiting in liver (0.988) and kidney (0.625) but Leu (0.459) in heart. In pre-school requirements, liver recorded no limiting amino acid whereas Thr was limiting in kidney (0.735) and Leu was limiting in the heart (0.486). T.swingerianus red viscera was compared with the red viscera of livestock animals (cattle, sheep and pork) as well as FAO/WHO/UNU standards for total essential amino acids. Our results when compared with the livestock red viscera (without Trp) and FAO/WHO/UNU (g100g-1 protein), we have heart: grasscutter/cattle/sheep/pig:45.3 /46.0/42.7/46.6; kidney: grasscutter/cattle/sheep/pig: 47.6/43.8/42.5/46.7; liver: grasscutter/cattle/sheep/pig: 50.7/47.7/41.5/47.5 and grasscutter liver/kidney/heart/ FAO/WHO/UNU:50.7/47.6/45.3/32.8 showing that all the red viscera values in T.swingerianus were better than the essential amino acids in the FAO/WHO/UNU standards and livestock red viscera. Statistical values showed that significant differences existed among the samples at r=0.01.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46215812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.18052/www.scipress.com/ilns.79.1
İbrahim Teber, E. Bursal
Phenolic content and antioxidant activity of Nepeta nuda subsp. albiflora Boiss. were reported in this study. The ethanol and water extracts of Nepeta nuda subsp. albiflora were prepared and used for biochemical analyses. Antioxidant capacities of the extracts were evaluated by three different in vitro bioanalytical methods including a reducing antioxidant method and two radical scavenging antioxidant methods. The water and ethanol extracts of the plant sample were found to have effective antioxidant potentials. Phenolic content of Nepeta nuda subsp. albiflora was determined by high performance liquid chromatography (HPLC). Rosmarinic acid (182.0±4.5 µg/g), apigenin (84.5±57.6 µg/g), and quercetin (44.5±62.9 µg/g) were identified as major compounds in the ethanol extract of the plant sample. This study has a potential scientific base for further studies about Nepeta nuda subsp. albiflora related to plant biochemistry and plant based pharmacological industry.
{"title":"Phenolic Compounds and Antioxidant Activity of Nepeta nuda subsp. Albiflora","authors":"İbrahim Teber, E. Bursal","doi":"10.18052/www.scipress.com/ilns.79.1","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.79.1","url":null,"abstract":"Phenolic content and antioxidant activity of Nepeta nuda subsp. albiflora Boiss. were reported in this study. The ethanol and water extracts of Nepeta nuda subsp. albiflora were prepared and used for biochemical analyses. Antioxidant capacities of the extracts were evaluated by three different in vitro bioanalytical methods including a reducing antioxidant method and two radical scavenging antioxidant methods. The water and ethanol extracts of the plant sample were found to have effective antioxidant potentials. Phenolic content of Nepeta nuda subsp. albiflora was determined by high performance liquid chromatography (HPLC). Rosmarinic acid (182.0±4.5 µg/g), apigenin (84.5±57.6 µg/g), and quercetin (44.5±62.9 µg/g) were identified as major compounds in the ethanol extract of the plant sample. This study has a potential scientific base for further studies about Nepeta nuda subsp. albiflora related to plant biochemistry and plant based pharmacological industry.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47074033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.18052/www.scipress.com/ilns.79.40
Emalina L. Ebol, Carlos H. Donoso, R. Saura, Rolit Joan C. Ferol, Juliet Ruth D. Mozar, Arman N. Bermon, Jerry B Manongas, Joan Christine H. Libot, Cara Jane Matabilas, J. Jumawan, R. Capangpangan
Lake Mainit is one of the largest lakes recognized as Key Biodiversity Areas (KBA) in the Philippines with rich fishery resources. However, the lake is at risk from heavy metal contamination due to inputs of industrial, agricultural effluents and small-scale mining activities. The present work evaluated levels of heavy metals namely cadmium, lead, and mercury from key aquatic fauna and sediments from seven strategic sections of the lake in 2018. Muscle samples of all seven fish species assessed were below detections limits (BDL) for tHg and Cd. Trace concentrations of Pb in the muscles were detected in Oreochromis niloticus, Glossogobius giuris, Channa striata and Vivipara angularis but values were within safe ranges. Trace concentrations of Pb in the riverine crab (Sundathelpusa sp) exceeded safe limits. Both Cd and tHg were below detection limits in the three invertebrates assessed. Traces of Pb were detected in S4 (Magtiaco) and S5 (Jaliobong) below standard limits (0.05 ppm) only during the southwest (SW) monsoon but Pb were not detected across all stations during the NE monsoon of 2018. For Cd, however, trace concentrations were detected only during the NE monsoon wherein Cd in S2 (Mayag), S3 (Magpayang), S4 (Magtiaco), S5 (Jaliobong), S6 (Dinarawan) and S7 (Kalinawan) exceeded standard limits for Cd in waters (0.01 ppm). Concentrations of tHg in the water were not detected across the two sampling seasons in all seven tributary stations. In sediments, Pb were all detected during the southwest monsoon with highest Pb concentrations in S6 (Dinarawan) and S7 (Kalinawan) which exceeded safe limits. Trace Cd in sediments were mostly below detectable limits. Concentrations of tHg in sediments exceeded safe limits during the SE monsoon in S4 (Magtiaco) and S7 (Kalinawan) areas. These findings recommended that continuous heavy metal monitoring must be conducted. It is also strongly suggested to evaluate the presence of heavy metals in other aquatic organisms and assess the ecological risk posed by these heavy metals though heavy metal speciation analysis.
{"title":"Heavy Metals Accumulation in Surface Waters, Bottom Sediments and Aquatic Organisms in Lake Mainit, Philippines","authors":"Emalina L. Ebol, Carlos H. Donoso, R. Saura, Rolit Joan C. Ferol, Juliet Ruth D. Mozar, Arman N. Bermon, Jerry B Manongas, Joan Christine H. Libot, Cara Jane Matabilas, J. Jumawan, R. Capangpangan","doi":"10.18052/www.scipress.com/ilns.79.40","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.79.40","url":null,"abstract":"Lake Mainit is one of the largest lakes recognized as Key Biodiversity Areas (KBA) in the Philippines with rich fishery resources. However, the lake is at risk from heavy metal contamination due to inputs of industrial, agricultural effluents and small-scale mining activities. The present work evaluated levels of heavy metals namely cadmium, lead, and mercury from key aquatic fauna and sediments from seven strategic sections of the lake in 2018. Muscle samples of all seven fish species assessed were below detections limits (BDL) for tHg and Cd. Trace concentrations of Pb in the muscles were detected in Oreochromis niloticus, Glossogobius giuris, Channa striata and Vivipara angularis but values were within safe ranges. Trace concentrations of Pb in the riverine crab (Sundathelpusa sp) exceeded safe limits. Both Cd and tHg were below detection limits in the three invertebrates assessed. Traces of Pb were detected in S4 (Magtiaco) and S5 (Jaliobong) below standard limits (0.05 ppm) only during the southwest (SW) monsoon but Pb were not detected across all stations during the NE monsoon of 2018. For Cd, however, trace concentrations were detected only during the NE monsoon wherein Cd in S2 (Mayag), S3 (Magpayang), S4 (Magtiaco), S5 (Jaliobong), S6 (Dinarawan) and S7 (Kalinawan) exceeded standard limits for Cd in waters (0.01 ppm). Concentrations of tHg in the water were not detected across the two sampling seasons in all seven tributary stations. In sediments, Pb were all detected during the southwest monsoon with highest Pb concentrations in S6 (Dinarawan) and S7 (Kalinawan) which exceeded safe limits. Trace Cd in sediments were mostly below detectable limits. Concentrations of tHg in sediments exceeded safe limits during the SE monsoon in S4 (Magtiaco) and S7 (Kalinawan) areas. These findings recommended that continuous heavy metal monitoring must be conducted. It is also strongly suggested to evaluate the presence of heavy metals in other aquatic organisms and assess the ecological risk posed by these heavy metals though heavy metal speciation analysis.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":"1 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41728957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-07-01DOI: 10.18052/www.scipress.com/ilns.79.16
O. Marenkov, O. Nesterenko
The article presents the results of studies on the content of artificial (137Cs, 90Sr) and natural radionuclides (226Ra, 232Th, 40K) in tissues and organs of various individuals of pumpkinseed Lepomisgibbosus (Linnaeus, 1758) from the Zaporizhzhia (Dnipro) reservoir. It has been revealed that in the bones of sexually mature pumpkinseed females, the content of radionuclides 137Cs and90Sr is 24.7% and 28.6% less, respectively, than in the bones of males. Summing up the materials of radioecological studies of 2017–2018, the following sequence of tissues and organs of the pumpkinseed by the ability to accumulate radionuclides was obtained: bone> muscle> scales> gills. Research results can be useful for further study on the fish radiation load in the basins of the Dnipro River and during monitoring radioecological research. It was determined that the content of radionuclides in the pumpkinseed muscles did not exceed the permissible levels of radionuclide content for fish as a food product (permissible levels of radionuclide content for 137Cs – 150 Bq/kg, 90Sr – 35 Bq/kg).
{"title":"Radionuclides Content in Tissues and Organs of Pumpkinseed Lepomis gibbosus (Linnaeus, 1758) from the Zaporizhzhia (Dnipro) Reservoir","authors":"O. Marenkov, O. Nesterenko","doi":"10.18052/www.scipress.com/ilns.79.16","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.79.16","url":null,"abstract":"The article presents the results of studies on the content of artificial (137Cs, 90Sr) and natural radionuclides (226Ra, 232Th, 40K) in tissues and organs of various individuals of pumpkinseed Lepomisgibbosus (Linnaeus, 1758) from the Zaporizhzhia (Dnipro) reservoir. It has been revealed that in the bones of sexually mature pumpkinseed females, the content of radionuclides 137Cs and90Sr is 24.7% and 28.6% less, respectively, than in the bones of males. Summing up the materials of radioecological studies of 2017–2018, the following sequence of tissues and organs of the pumpkinseed by the ability to accumulate radionuclides was obtained: bone> muscle> scales> gills. Research results can be useful for further study on the fish radiation load in the basins of the Dnipro River and during monitoring radioecological research. It was determined that the content of radionuclides in the pumpkinseed muscles did not exceed the permissible levels of radionuclide content for fish as a food product (permissible levels of radionuclide content for 137Cs – 150 Bq/kg, 90Sr – 35 Bq/kg).","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46742304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-01DOI: 10.18052/www.scipress.com/ilns.78.34
Y. Yohannes, O. Shibistova, G. Guggenberger
Tree species differ in litter quality and belowground biomass, thereby exerting species-specific impact on soil properties and microbial biomass. A study was conducted to find out the comparative effects of Podocarpus falcatus and Croton macrostachys on basic soil characteristics and microbial biomass, in the Munessa forest, Ethiopia. Four experimental plots under the canopies the respected tree species (two from each) were established for sample collection. From these plots, soil samples were collected from a depth 0-10 cm and 10-25 cm. The results showed that, from the depth 0-10 cm, concentration of organic carbon (C) and nitrogen (N) was larger under C. macrostachys and from the depth 10-25 cm these values were greater under P. falcatus. There was significant difference (p < 0.05) in cation exchange capacity being larger under C. macrostachys. There were no differences in microbial composition between the plots. However, the total phospholipid fatty acids (PLFA) concentration as an entry for microbial biomass determination tended to be significantly larger in soil under Podocarpus plots (382.7 ± 60.9 nmol PLFA g-1 dry soil) vs. 262.2 ± 32.8 nmol PLFA g-1 dry soil (Croton plots). The varying impacts of tree species on soil characteristics and microbial biomass may be partly explained by differences in functional traits related to life-history strategy of the respected species.
{"title":"Comparative Effects of Dominant Forest Tree Species on Soil Characteristics and Microbial Biomass","authors":"Y. Yohannes, O. Shibistova, G. Guggenberger","doi":"10.18052/www.scipress.com/ilns.78.34","DOIUrl":"https://doi.org/10.18052/www.scipress.com/ilns.78.34","url":null,"abstract":"Tree species differ in litter quality and belowground biomass, thereby exerting species-specific impact on soil properties and microbial biomass. A study was conducted to find out the comparative effects of Podocarpus falcatus and Croton macrostachys on basic soil characteristics and microbial biomass, in the Munessa forest, Ethiopia. Four experimental plots under the canopies the respected tree species (two from each) were established for sample collection. From these plots, soil samples were collected from a depth 0-10 cm and 10-25 cm. The results showed that, from the depth 0-10 cm, concentration of organic carbon (C) and nitrogen (N) was larger under C. macrostachys and from the depth 10-25 cm these values were greater under P. falcatus. There was significant difference (p < 0.05) in cation exchange capacity being larger under C. macrostachys. There were no differences in microbial composition between the plots. However, the total phospholipid fatty acids (PLFA) concentration as an entry for microbial biomass determination tended to be significantly larger in soil under Podocarpus plots (382.7 ± 60.9 nmol PLFA g-1 dry soil) vs. 262.2 ± 32.8 nmol PLFA g-1 dry soil (Croton plots). The varying impacts of tree species on soil characteristics and microbial biomass may be partly explained by differences in functional traits related to life-history strategy of the respected species.","PeriodicalId":14407,"journal":{"name":"International Letters of Natural Sciences","volume":" ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47681302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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