Pub Date : 2019-10-11DOI: 10.26650/ISTANBULJPHARM.2018.180003
M. Z. Aumeeruddy, Z. Aumeeruddy-Elalfi, H. Neetoo, G. Zengin, B. Fibrich, S. Rademan, A. B. V. Staden, K. Szuman, I. Lambrechts, N. Lall, M. Mahomoodally
This study was designed to evaluate and correlate the biological, phytochemical, and physicochemical properties of two NSS (Nigella sativa seed) oils (N1 and N2). The biological activity was evaluated in terms of antibacterial, antioxidant (nitric oxide scavenging), antielastase, antityrosinase, antimelanogenic, and anticancer activity. Physicochemical properties including colour, total soluble solids (TSS), and density were also investigated. N2 exhibited higher antibacterial, NO scavenging, extracellular antimelanogenic, and anticancer activity against the HeLa cell line compared to N1, which showed higher antityrosinase activity. TPC (total phenolic content) and TFC (total flavonoid content) of N1 were significantly higher than N2 while TTC (total tannin content) was higher in N2. In fact, TTC was strongly correlated (R=1.000) with the antioxidant, extracellular antimelanogenic, and HeLa cell inhibitory activity. To conclude, NSS oil may be considered as a complementary and alternative therapy in the management of infectious and chronic diseases but warrants further in vivo pharmacological validation and toxicological analysis.
{"title":"Biological, phytochemical, and physico-chemical properties of two commercial Nigella sativa seed oils: A comparative analysis","authors":"M. Z. Aumeeruddy, Z. Aumeeruddy-Elalfi, H. Neetoo, G. Zengin, B. Fibrich, S. Rademan, A. B. V. Staden, K. Szuman, I. Lambrechts, N. Lall, M. Mahomoodally","doi":"10.26650/ISTANBULJPHARM.2018.180003","DOIUrl":"https://doi.org/10.26650/ISTANBULJPHARM.2018.180003","url":null,"abstract":"This study was designed to evaluate and correlate the biological, phytochemical, and physicochemical properties of two NSS (Nigella sativa seed) oils (N1 and N2). The biological activity was evaluated in terms of antibacterial, antioxidant (nitric oxide scavenging), antielastase, antityrosinase, antimelanogenic, and anticancer activity. Physicochemical properties including colour, total soluble solids (TSS), and density were also investigated. N2 exhibited higher antibacterial, NO scavenging, extracellular antimelanogenic, and anticancer activity against the HeLa cell line compared to N1, which showed higher antityrosinase activity. TPC (total phenolic content) and TFC (total flavonoid content) of N1 were significantly higher than N2 while TTC (total tannin content) was higher in N2. In fact, TTC was strongly correlated (R=1.000) with the antioxidant, extracellular antimelanogenic, and HeLa cell inhibitory activity. To conclude, NSS oil may be considered as a complementary and alternative therapy in the management of infectious and chronic diseases but warrants further in vivo pharmacological validation and toxicological analysis.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75281778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-11DOI: 10.26650/ISTANBULJPHARM.2018.400730
Ozlem Oyardi, P. Savage, Alper Akçalı, Z. Erturan, C. Bozkurt-Guzel
DOI : 10.26650/IstanbulJPharm.2018.400730 Ceragenins are novel promising agents for the treatment of infections caused by multi-drug resistant microorganisms. Since colistin resistance has become a worldwide problem, the need for new treatment agents has been increasing steadily. Therefore, this study aimed to investigate in vitro antimicrobial activities of ceragenins (Cationic Steroid Antibiotics) (CSA-8, CSA-13, CSA-142 and CSA-192) against multidrug resistant Gram negative isolates from Turkey. Experiments were performed by using broth microdilution method against Klebsiella pneumoniae, Morganella morganii, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates. All microorganisms except for three isolates were identified as multidrug resistant. Among tested ceragenins, CSA-13 showed the best results (MIC: 8-64 µg/ml). Nevertheless, the antimicrobial activity of CSA-8 was not significant. In conclusion, ceragenins appear to be a good candidate as antimicrobial therapy in the presence of multidrug (including colistin) resistant microorganisms.
{"title":"Ceragenins exhibiting promising antimicrobial activity against various multidrug resistant Gram negative bacteria","authors":"Ozlem Oyardi, P. Savage, Alper Akçalı, Z. Erturan, C. Bozkurt-Guzel","doi":"10.26650/ISTANBULJPHARM.2018.400730","DOIUrl":"https://doi.org/10.26650/ISTANBULJPHARM.2018.400730","url":null,"abstract":"DOI : 10.26650/IstanbulJPharm.2018.400730 Ceragenins are novel promising agents for the treatment of infections caused by multi-drug resistant microorganisms. Since colistin resistance has become a worldwide problem, the need for new treatment agents has been increasing steadily. Therefore, this study aimed to investigate in vitro antimicrobial activities of ceragenins (Cationic Steroid Antibiotics) (CSA-8, CSA-13, CSA-142 and CSA-192) against multidrug resistant Gram negative isolates from Turkey. Experiments were performed by using broth microdilution method against Klebsiella pneumoniae, Morganella morganii, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates. All microorganisms except for three isolates were identified as multidrug resistant. Among tested ceragenins, CSA-13 showed the best results (MIC: 8-64 µg/ml). Nevertheless, the antimicrobial activity of CSA-8 was not significant. In conclusion, ceragenins appear to be a good candidate as antimicrobial therapy in the presence of multidrug (including colistin) resistant microorganisms.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78842272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-11DOI: 10.5152/ISTANBULJPHARM.2018.376278
Y. Yeşil, Zeynep Büşra Erarslan
DOI : 10.5152/IstanbulJPharm.2018.376278 Scabiosa atropurpurea L. (Caprifoliaceae), a medicinal plant traditionally known as “Mor uyuzotu or Şeytanotu” in Turkey, has been used for treating acne, bronchitis, cold, and cough. Although phytochemical studies are available, there are no taxonomic studies related to this plant. The aim of this study was to investigate the anatomy of leaf, stem, and root of S. atropurpurea. Detailed photographs of plant parts were taken, and measurements of the leaf, stem, and root were carried out. The cross section of the stem revealed a single-layered epidermis containing one-celled simple eglandular trichomes and rarely one-celled hook-shaped eglandular trichomes. The cortex showed multilayered parenchymatous cells and a single-layered endodermis. The phloem was followed by the cambium, and the xylem was lignified. The pith was made up of parenchymatous cells. The leaves are bifacial and amphistomatic and stomata are mesomorphic in the cross section and also stomata are anomocytic in the surface section. The cross section of the root showed that the epidermis was replaced with the periderm. Under the phloem, which had few layers, the xylem was composed of tracheary elements surrounded with sclerenchymatous cells.
{"title":"The anatomical properties of Scabiosa atropurpurea L. (Caprifoliaceae)","authors":"Y. Yeşil, Zeynep Büşra Erarslan","doi":"10.5152/ISTANBULJPHARM.2018.376278","DOIUrl":"https://doi.org/10.5152/ISTANBULJPHARM.2018.376278","url":null,"abstract":"DOI : 10.5152/IstanbulJPharm.2018.376278 Scabiosa atropurpurea L. (Caprifoliaceae), a medicinal plant traditionally known as “Mor uyuzotu or Şeytanotu” in Turkey, has been used for treating acne, bronchitis, cold, and cough. Although phytochemical studies are available, there are no taxonomic studies related to this plant. The aim of this study was to investigate the anatomy of leaf, stem, and root of S. atropurpurea. Detailed photographs of plant parts were taken, and measurements of the leaf, stem, and root were carried out. The cross section of the stem revealed a single-layered epidermis containing one-celled simple eglandular trichomes and rarely one-celled hook-shaped eglandular trichomes. The cortex showed multilayered parenchymatous cells and a single-layered endodermis. The phloem was followed by the cambium, and the xylem was lignified. The pith was made up of parenchymatous cells. The leaves are bifacial and amphistomatic and stomata are mesomorphic in the cross section and also stomata are anomocytic in the surface section. The cross section of the root showed that the epidermis was replaced with the periderm. Under the phloem, which had few layers, the xylem was composed of tracheary elements surrounded with sclerenchymatous cells.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81620078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-11DOI: 10.5152/ISTANBULJPHARM.2018.382627
E. Acar, Gozde Hasbal, N. Ozsoy
DOI : 10.5152/IstanbulJPharm.2018.382627 Thioredoxin reductase (TrxR, EC 1.6.4.5) is a ubiquitous flavoenzyme that is present from Archaea to humans, and it is the only enzyme capable of catalyzing the reduction of thioredoxin (Trx) by nicotinamide adenine dinucleotide phosphate (NADPH). Although TrxR has been purified and characterized from different bacteria, plants, and mammalian organisms, a survey of the literature revealed no studies on the purification and characterization of TrxR from the mussel Mytilus galloprovincialis Lam. In this study, TrxR was purified to homogeneity from the hepatopancreatic tissue of M. galloprovincialis Lam. by extraction, ammonium sulfate precipitation, and DEAE-Sepharose CL-6B anion and 2’,5‘-ADP-agarose chromatographies, and some of its kinetic properties were examined. Molar mass determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed only a single protein band corresponding to a molecular weight of 35 kDa. Optimum pH and temperature were found to be 7.0 and 60°C, respectively. Km and Vmax values for NADPH were found to be 85 µmol and 4.82 µmol/min/mg, respectively. For 5,5ʹ-dithiobis (2-nitrobenzoic) acid (DTNB), the Km and Vmax values were 193 µmol and 1.32 µmol/min/mg, respectively. Increasing the knowledge on the kinetic properties of TrxR will significantly increase the prospects of enzyme application as an oxidative stress biomarker in mussels and fishes for monitoring contamination in coastal environments.
{"title":"Purification and partial characterization of thioredoxin reductase from the\u0000 hepatopancreas of the mollusc Mytilus galloprovincialis Lam.","authors":"E. Acar, Gozde Hasbal, N. Ozsoy","doi":"10.5152/ISTANBULJPHARM.2018.382627","DOIUrl":"https://doi.org/10.5152/ISTANBULJPHARM.2018.382627","url":null,"abstract":"DOI : 10.5152/IstanbulJPharm.2018.382627 Thioredoxin reductase (TrxR, EC 1.6.4.5) is a ubiquitous flavoenzyme that is present from Archaea to humans, and it is the only enzyme capable of catalyzing the reduction of thioredoxin (Trx) by nicotinamide adenine dinucleotide phosphate (NADPH). Although TrxR has been purified and characterized from different bacteria, plants, and mammalian organisms, a survey of the literature revealed no studies on the purification and characterization of TrxR from the mussel Mytilus galloprovincialis Lam. In this study, TrxR was purified to homogeneity from the hepatopancreatic tissue of M. galloprovincialis Lam. by extraction, ammonium sulfate precipitation, and DEAE-Sepharose CL-6B anion and 2’,5‘-ADP-agarose chromatographies, and some of its kinetic properties were examined. Molar mass determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed only a single protein band corresponding to a molecular weight of 35 kDa. Optimum pH and temperature were found to be 7.0 and 60°C, respectively. Km and Vmax values for NADPH were found to be 85 µmol and 4.82 µmol/min/mg, respectively. For 5,5ʹ-dithiobis (2-nitrobenzoic) acid (DTNB), the Km and Vmax values were 193 µmol and 1.32 µmol/min/mg, respectively. Increasing the knowledge on the kinetic properties of TrxR will significantly increase the prospects of enzyme application as an oxidative stress biomarker in mussels and fishes for monitoring contamination in coastal environments.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85789657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}