Pub Date : 2019-10-11DOI: 10.26650/ISTANBULJPHARM.2018.180003
M. Z. Aumeeruddy, Z. Aumeeruddy-Elalfi, H. Neetoo, G. Zengin, B. Fibrich, S. Rademan, A. B. V. Staden, K. Szuman, I. Lambrechts, N. Lall, M. Mahomoodally
This study was designed to evaluate and correlate the biological, phytochemical, and physicochemical properties of two NSS (Nigella sativa seed) oils (N1 and N2). The biological activity was evaluated in terms of antibacterial, antioxidant (nitric oxide scavenging), antielastase, antityrosinase, antimelanogenic, and anticancer activity. Physicochemical properties including colour, total soluble solids (TSS), and density were also investigated. N2 exhibited higher antibacterial, NO scavenging, extracellular antimelanogenic, and anticancer activity against the HeLa cell line compared to N1, which showed higher antityrosinase activity. TPC (total phenolic content) and TFC (total flavonoid content) of N1 were significantly higher than N2 while TTC (total tannin content) was higher in N2. In fact, TTC was strongly correlated (R=1.000) with the antioxidant, extracellular antimelanogenic, and HeLa cell inhibitory activity. To conclude, NSS oil may be considered as a complementary and alternative therapy in the management of infectious and chronic diseases but warrants further in vivo pharmacological validation and toxicological analysis.
{"title":"Biological, phytochemical, and physico-chemical properties of two commercial Nigella sativa seed oils: A comparative analysis","authors":"M. Z. Aumeeruddy, Z. Aumeeruddy-Elalfi, H. Neetoo, G. Zengin, B. Fibrich, S. Rademan, A. B. V. Staden, K. Szuman, I. Lambrechts, N. Lall, M. Mahomoodally","doi":"10.26650/ISTANBULJPHARM.2018.180003","DOIUrl":"https://doi.org/10.26650/ISTANBULJPHARM.2018.180003","url":null,"abstract":"This study was designed to evaluate and correlate the biological, phytochemical, and physicochemical properties of two NSS (Nigella sativa seed) oils (N1 and N2). The biological activity was evaluated in terms of antibacterial, antioxidant (nitric oxide scavenging), antielastase, antityrosinase, antimelanogenic, and anticancer activity. Physicochemical properties including colour, total soluble solids (TSS), and density were also investigated. N2 exhibited higher antibacterial, NO scavenging, extracellular antimelanogenic, and anticancer activity against the HeLa cell line compared to N1, which showed higher antityrosinase activity. TPC (total phenolic content) and TFC (total flavonoid content) of N1 were significantly higher than N2 while TTC (total tannin content) was higher in N2. In fact, TTC was strongly correlated (R=1.000) with the antioxidant, extracellular antimelanogenic, and HeLa cell inhibitory activity. To conclude, NSS oil may be considered as a complementary and alternative therapy in the management of infectious and chronic diseases but warrants further in vivo pharmacological validation and toxicological analysis.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75281778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-11DOI: 10.26650/ISTANBULJPHARM.2018.400730
Ozlem Oyardi, P. Savage, Alper Akçalı, Z. Erturan, C. Bozkurt-Guzel
DOI : 10.26650/IstanbulJPharm.2018.400730 Ceragenins are novel promising agents for the treatment of infections caused by multi-drug resistant microorganisms. Since colistin resistance has become a worldwide problem, the need for new treatment agents has been increasing steadily. Therefore, this study aimed to investigate in vitro antimicrobial activities of ceragenins (Cationic Steroid Antibiotics) (CSA-8, CSA-13, CSA-142 and CSA-192) against multidrug resistant Gram negative isolates from Turkey. Experiments were performed by using broth microdilution method against Klebsiella pneumoniae, Morganella morganii, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates. All microorganisms except for three isolates were identified as multidrug resistant. Among tested ceragenins, CSA-13 showed the best results (MIC: 8-64 µg/ml). Nevertheless, the antimicrobial activity of CSA-8 was not significant. In conclusion, ceragenins appear to be a good candidate as antimicrobial therapy in the presence of multidrug (including colistin) resistant microorganisms.
{"title":"Ceragenins exhibiting promising antimicrobial activity against various multidrug resistant Gram negative bacteria","authors":"Ozlem Oyardi, P. Savage, Alper Akçalı, Z. Erturan, C. Bozkurt-Guzel","doi":"10.26650/ISTANBULJPHARM.2018.400730","DOIUrl":"https://doi.org/10.26650/ISTANBULJPHARM.2018.400730","url":null,"abstract":"DOI : 10.26650/IstanbulJPharm.2018.400730 Ceragenins are novel promising agents for the treatment of infections caused by multi-drug resistant microorganisms. Since colistin resistance has become a worldwide problem, the need for new treatment agents has been increasing steadily. Therefore, this study aimed to investigate in vitro antimicrobial activities of ceragenins (Cationic Steroid Antibiotics) (CSA-8, CSA-13, CSA-142 and CSA-192) against multidrug resistant Gram negative isolates from Turkey. Experiments were performed by using broth microdilution method against Klebsiella pneumoniae, Morganella morganii, Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolates. All microorganisms except for three isolates were identified as multidrug resistant. Among tested ceragenins, CSA-13 showed the best results (MIC: 8-64 µg/ml). Nevertheless, the antimicrobial activity of CSA-8 was not significant. In conclusion, ceragenins appear to be a good candidate as antimicrobial therapy in the presence of multidrug (including colistin) resistant microorganisms.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78842272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-11DOI: 10.5152/ISTANBULJPHARM.2018.376278
Y. Yeşil, Zeynep Büşra Erarslan
DOI : 10.5152/IstanbulJPharm.2018.376278 Scabiosa atropurpurea L. (Caprifoliaceae), a medicinal plant traditionally known as “Mor uyuzotu or Şeytanotu” in Turkey, has been used for treating acne, bronchitis, cold, and cough. Although phytochemical studies are available, there are no taxonomic studies related to this plant. The aim of this study was to investigate the anatomy of leaf, stem, and root of S. atropurpurea. Detailed photographs of plant parts were taken, and measurements of the leaf, stem, and root were carried out. The cross section of the stem revealed a single-layered epidermis containing one-celled simple eglandular trichomes and rarely one-celled hook-shaped eglandular trichomes. The cortex showed multilayered parenchymatous cells and a single-layered endodermis. The phloem was followed by the cambium, and the xylem was lignified. The pith was made up of parenchymatous cells. The leaves are bifacial and amphistomatic and stomata are mesomorphic in the cross section and also stomata are anomocytic in the surface section. The cross section of the root showed that the epidermis was replaced with the periderm. Under the phloem, which had few layers, the xylem was composed of tracheary elements surrounded with sclerenchymatous cells.
{"title":"The anatomical properties of Scabiosa atropurpurea L. (Caprifoliaceae)","authors":"Y. Yeşil, Zeynep Büşra Erarslan","doi":"10.5152/ISTANBULJPHARM.2018.376278","DOIUrl":"https://doi.org/10.5152/ISTANBULJPHARM.2018.376278","url":null,"abstract":"DOI : 10.5152/IstanbulJPharm.2018.376278 Scabiosa atropurpurea L. (Caprifoliaceae), a medicinal plant traditionally known as “Mor uyuzotu or Şeytanotu” in Turkey, has been used for treating acne, bronchitis, cold, and cough. Although phytochemical studies are available, there are no taxonomic studies related to this plant. The aim of this study was to investigate the anatomy of leaf, stem, and root of S. atropurpurea. Detailed photographs of plant parts were taken, and measurements of the leaf, stem, and root were carried out. The cross section of the stem revealed a single-layered epidermis containing one-celled simple eglandular trichomes and rarely one-celled hook-shaped eglandular trichomes. The cortex showed multilayered parenchymatous cells and a single-layered endodermis. The phloem was followed by the cambium, and the xylem was lignified. The pith was made up of parenchymatous cells. The leaves are bifacial and amphistomatic and stomata are mesomorphic in the cross section and also stomata are anomocytic in the surface section. The cross section of the root showed that the epidermis was replaced with the periderm. Under the phloem, which had few layers, the xylem was composed of tracheary elements surrounded with sclerenchymatous cells.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81620078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-11DOI: 10.5152/ISTANBULJPHARM.2018.382627
E. Acar, Gozde Hasbal, N. Ozsoy
DOI : 10.5152/IstanbulJPharm.2018.382627 Thioredoxin reductase (TrxR, EC 1.6.4.5) is a ubiquitous flavoenzyme that is present from Archaea to humans, and it is the only enzyme capable of catalyzing the reduction of thioredoxin (Trx) by nicotinamide adenine dinucleotide phosphate (NADPH). Although TrxR has been purified and characterized from different bacteria, plants, and mammalian organisms, a survey of the literature revealed no studies on the purification and characterization of TrxR from the mussel Mytilus galloprovincialis Lam. In this study, TrxR was purified to homogeneity from the hepatopancreatic tissue of M. galloprovincialis Lam. by extraction, ammonium sulfate precipitation, and DEAE-Sepharose CL-6B anion and 2’,5‘-ADP-agarose chromatographies, and some of its kinetic properties were examined. Molar mass determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed only a single protein band corresponding to a molecular weight of 35 kDa. Optimum pH and temperature were found to be 7.0 and 60°C, respectively. Km and Vmax values for NADPH were found to be 85 µmol and 4.82 µmol/min/mg, respectively. For 5,5ʹ-dithiobis (2-nitrobenzoic) acid (DTNB), the Km and Vmax values were 193 µmol and 1.32 µmol/min/mg, respectively. Increasing the knowledge on the kinetic properties of TrxR will significantly increase the prospects of enzyme application as an oxidative stress biomarker in mussels and fishes for monitoring contamination in coastal environments.
{"title":"Purification and partial characterization of thioredoxin reductase from the\u0000 hepatopancreas of the mollusc Mytilus galloprovincialis Lam.","authors":"E. Acar, Gozde Hasbal, N. Ozsoy","doi":"10.5152/ISTANBULJPHARM.2018.382627","DOIUrl":"https://doi.org/10.5152/ISTANBULJPHARM.2018.382627","url":null,"abstract":"DOI : 10.5152/IstanbulJPharm.2018.382627 Thioredoxin reductase (TrxR, EC 1.6.4.5) is a ubiquitous flavoenzyme that is present from Archaea to humans, and it is the only enzyme capable of catalyzing the reduction of thioredoxin (Trx) by nicotinamide adenine dinucleotide phosphate (NADPH). Although TrxR has been purified and characterized from different bacteria, plants, and mammalian organisms, a survey of the literature revealed no studies on the purification and characterization of TrxR from the mussel Mytilus galloprovincialis Lam. In this study, TrxR was purified to homogeneity from the hepatopancreatic tissue of M. galloprovincialis Lam. by extraction, ammonium sulfate precipitation, and DEAE-Sepharose CL-6B anion and 2’,5‘-ADP-agarose chromatographies, and some of its kinetic properties were examined. Molar mass determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed only a single protein band corresponding to a molecular weight of 35 kDa. Optimum pH and temperature were found to be 7.0 and 60°C, respectively. Km and Vmax values for NADPH were found to be 85 µmol and 4.82 µmol/min/mg, respectively. For 5,5ʹ-dithiobis (2-nitrobenzoic) acid (DTNB), the Km and Vmax values were 193 µmol and 1.32 µmol/min/mg, respectively. Increasing the knowledge on the kinetic properties of TrxR will significantly increase the prospects of enzyme application as an oxidative stress biomarker in mussels and fishes for monitoring contamination in coastal environments.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85789657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-11DOI: 10.26650/ISTANBULJPHARM.2018.18007
S. Türk, K. Turan, S. Ulusoy, S. Karakuş, G. Boşgelmez-Tinaz
DOI : 10.26650/IstanbulJPharm.2018.18007 In this study, with intent to find out novel anti-biofilm and antiviral agents, a series of amide derivatives were synthesized and their structures were elucidated by FT-IR and 1H-NMR and 13C-NMR and MS methods. And also, their purity were proven by TLC, HPLC and elemental analyses. And finally, the synthesized compounds were examined for their biofilm formation and swarming motility inhibitory activities in P. aeruginosa PA01. These compounds were found to reduce biofilm formation by 8.7-25.6% and swarming motility by 18.3-33.8% in P. aeruginosa PA01 at a concentration of 200 μM. Besides, all of the compounds were evaluated for their antiviral activity against influenza A viruses. The plaque inhibition assays indicated that compound 6 (N-(4-{[5-(ethylamino)-1,3,4-thiadiazol-2-yl]methyl}phenyl)-4- fluorobenzamide) has considerable inhibitory effect on influenza A virus plaque formation.
{"title":"Synthesis, characterization and biological activity studies on amide derivatives","authors":"S. Türk, K. Turan, S. Ulusoy, S. Karakuş, G. Boşgelmez-Tinaz","doi":"10.26650/ISTANBULJPHARM.2018.18007","DOIUrl":"https://doi.org/10.26650/ISTANBULJPHARM.2018.18007","url":null,"abstract":"DOI : 10.26650/IstanbulJPharm.2018.18007 In this study, with intent to find out novel anti-biofilm and antiviral agents, a series of amide derivatives were synthesized and their structures were elucidated by FT-IR and 1H-NMR and 13C-NMR and MS methods. And also, their purity were proven by TLC, HPLC and elemental analyses. And finally, the synthesized compounds were examined for their biofilm formation and swarming motility inhibitory activities in P. aeruginosa PA01. These compounds were found to reduce biofilm formation by 8.7-25.6% and swarming motility by 18.3-33.8% in P. aeruginosa PA01 at a concentration of 200 μM. Besides, all of the compounds were evaluated for their antiviral activity against influenza A viruses. The plaque inhibition assays indicated that compound 6 (N-(4-{[5-(ethylamino)-1,3,4-thiadiazol-2-yl]methyl}phenyl)-4- fluorobenzamide) has considerable inhibitory effect on influenza A virus plaque formation.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75943755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-11DOI: 10.26650/ISTANBULJPHARM.2018.422525
Ç. Karakoyun
DOI : 10.26650/IstanbulJPharm.2018.422525 Galanthus L. is generally acknowledged as a valuable biological source due to its Amaryllidaceae alkaloids with unusual chemical structures and a wide variety of biological activities. Among these alkaloids, galanthamine is the first example of their practical application in medicine as a potent and selective inhibitor of the enzyme acetylcholinesterase, and already present on the market as a therapeutic agent for Alzheimer’s disease. Lycorine is the main Amaryllidaceae alkaloid deeply investigated for its biological activity for many years. In this study the alkaloidal profile of G. gracilis collected from western Aegean (Alankiyi/Bayindir) will be illustrated for the first time. GC-MS profiling was carried out on the crude alkaloidal extract obtained from dried and powdered plant material. 11 alkaloids were detected by GC-MS, including graciline, demethylhomolycorine and tazettine as the major ones.
{"title":"Alkaloid profiling in Galanthus gracilis Celak. from western Turkey by GC/MS","authors":"Ç. Karakoyun","doi":"10.26650/ISTANBULJPHARM.2018.422525","DOIUrl":"https://doi.org/10.26650/ISTANBULJPHARM.2018.422525","url":null,"abstract":"DOI : 10.26650/IstanbulJPharm.2018.422525 Galanthus L. is generally acknowledged as a valuable biological source due to its Amaryllidaceae alkaloids with unusual chemical structures and a wide variety of biological activities. Among these alkaloids, galanthamine is the first example of their practical application in medicine as a potent and selective inhibitor of the enzyme acetylcholinesterase, and already present on the market as a therapeutic agent for Alzheimer’s disease. Lycorine is the main Amaryllidaceae alkaloid deeply investigated for its biological activity for many years. In this study the alkaloidal profile of G. gracilis collected from western Aegean (Alankiyi/Bayindir) will be illustrated for the first time. GC-MS profiling was carried out on the crude alkaloidal extract obtained from dried and powdered plant material. 11 alkaloids were detected by GC-MS, including graciline, demethylhomolycorine and tazettine as the major ones.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87400765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-09DOI: 10.5152/ISTANBULJPHARM.2018.320135
S. Sarkar
DOI: 10.5152/IstanbulJPharm.2018.320135 Recent studies have demonstrated that 2-amino-substituted benzothiazole derivatives exhibit significant antitubercular activity. In this study, new compounds of benzothiazole-containing azetidinone derivatives were designed and synthesized using substituted benzaldehydeN-(5fluoro-1,3-benzothiazol-2-yl) semicarbazone, chloroacetyl chloride, and triethylamine in DMF. The structures of the synthesized compounds were characterized by TLC, IR, elemental analysis, and 1H NMR. In vitro screening data revealed that all the designed and synthesized compounds A6–A10 exhibited the ability to inhibit the growth of Mycobacterium tuberculosis in terms of MIC. Variable and modest activity was observed against the investigated strains of bacteria; however, the compound A6 exhibited significant antitubercular activity against M. tuberculosis H37Rv (MTCC 200) compared to that of the reference drugs isoniazid and rifampicin. These experimental data were consistent with our computational predictions in terms of the compound A6 that exhibited a satisfactory backbone for the antitubercular activity, perhaps due to an increase in hydrophobicity resulting in better penetration through the cell wall of M. tuberculosis.
{"title":"Design, synthesis, and evaluation of antitubercular activity of a novel benzothiazole-containing an azetidinone ring","authors":"S. Sarkar","doi":"10.5152/ISTANBULJPHARM.2018.320135","DOIUrl":"https://doi.org/10.5152/ISTANBULJPHARM.2018.320135","url":null,"abstract":"DOI: 10.5152/IstanbulJPharm.2018.320135 Recent studies have demonstrated that 2-amino-substituted benzothiazole derivatives exhibit significant antitubercular activity. In this study, new compounds of benzothiazole-containing azetidinone derivatives were designed and synthesized using substituted benzaldehydeN-(5fluoro-1,3-benzothiazol-2-yl) semicarbazone, chloroacetyl chloride, and triethylamine in DMF. The structures of the synthesized compounds were characterized by TLC, IR, elemental analysis, and 1H NMR. In vitro screening data revealed that all the designed and synthesized compounds A6–A10 exhibited the ability to inhibit the growth of Mycobacterium tuberculosis in terms of MIC. Variable and modest activity was observed against the investigated strains of bacteria; however, the compound A6 exhibited significant antitubercular activity against M. tuberculosis H37Rv (MTCC 200) compared to that of the reference drugs isoniazid and rifampicin. These experimental data were consistent with our computational predictions in terms of the compound A6 that exhibited a satisfactory backbone for the antitubercular activity, perhaps due to an increase in hydrophobicity resulting in better penetration through the cell wall of M. tuberculosis.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78049261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-09DOI: 10.5152/ISTANBULJPHARM.2018.397094
Cem Kaplan, D. Unal
DOI : 10.5152.IstanbulJPharm.2018.397094 A high performance liquid chromatography method has been developed for determination of letrozole in tablets. The method has developed in reverse phase column with acetonitrile-50.0 mM phosphoric acid solution, pH:7 (50:50, v/v) and 0.7 mL/min flow rate by using a fluorimetric detector. Letrozole has natural fluorescence properties, and there is no need for derivatization for this molecule. The detector was set at 256, 585 nm for excitation and emission wavelength respectively. The method was validated in accuracy, precision, specificity, linearity and system suitability parameters are studied. The linear range was found 50-700 ng/mL. The limit of detection and quantification for letrozole was found to be 14-50 ng/mL respectively. The recovery of drug is found to be 98.8%. The developed method has been validated and successfully applied to tablet analysis of drug substance. This method is simple, reproducible and can be used safely for routine analysis of letrozole in tablets.
{"title":"A validated method without derivatization for the determination of Letrozole by high performance liquid chromatography-fluorimetric method for pharmaceutical preparation","authors":"Cem Kaplan, D. Unal","doi":"10.5152/ISTANBULJPHARM.2018.397094","DOIUrl":"https://doi.org/10.5152/ISTANBULJPHARM.2018.397094","url":null,"abstract":"DOI : 10.5152.IstanbulJPharm.2018.397094 A high performance liquid chromatography method has been developed for determination of letrozole in tablets. The method has developed in reverse phase column with acetonitrile-50.0 mM phosphoric acid solution, pH:7 (50:50, v/v) and 0.7 mL/min flow rate by using a fluorimetric detector. Letrozole has natural fluorescence properties, and there is no need for derivatization for this molecule. The detector was set at 256, 585 nm for excitation and emission wavelength respectively. The method was validated in accuracy, precision, specificity, linearity and system suitability parameters are studied. The linear range was found 50-700 ng/mL. The limit of detection and quantification for letrozole was found to be 14-50 ng/mL respectively. The recovery of drug is found to be 98.8%. The developed method has been validated and successfully applied to tablet analysis of drug substance. This method is simple, reproducible and can be used safely for routine analysis of letrozole in tablets.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"220 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85015503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-09DOI: 10.5152/ISTANBULJPHARM.2018.412321
Meryem Seyda Erbay, S. Anıl, G. Melikoğlu
DOI : 10.5152/IstanbulJPharm.2018.412321 Turkey has a rich plant diversity due to its geography and many of these plants are used in the treatment of various diseases. The usage of these medicinal plants has been passed down from generation to generation. Ethnobotanical studies are used to record traditional treatment methods and this information is intended to contribute to drug development studies. This study (which is the fourth article in a series focusing on plants with painkilling properties) details the plants used to combat toothache. In this survey, which was prepared by screening ethnobotanical research, a total of 52 taxa were traditionally used in toothache treatment in Turkey. Scientific and local names, families, parts used, and treatment methods of these plants were recorded. According to the results of the research, the most common families are Lamiaceae, Asteraceae, Solanaceae, Euphorbiaceae, Liliaceae and Rosaceae. The most commonly used taxa in different regions of Turkey are Cornus mas, Dianthus zonatus var. zonatus and Hyocyamus niger. These plants contain analgesic and anti-inflammatory compounds. These plants are usually used externally as extracts (infusion/decoction) as well as are used directly. However, a few species are also used externally.
由于其地理位置，土耳其拥有丰富的植物多样性，其中许多植物被用于治疗各种疾病。这些药用植物的用法是代代相传的。民族植物学研究用于记录传统的治疗方法，这些信息旨在为药物开发研究做出贡献。这项研究(这是关注具有止痛特性的植物系列的第四篇文章)详细介绍了用于治疗牙痛的植物。在本次调查中，通过筛选民族植物学研究，土耳其共有52个分类群传统上用于牙痛治疗。记录了这些植物的学名和地名、科、使用部位和处理方法。根据研究结果，最常见的科是兰科、菊科、茄科、大戟科、百合科和蔷薇科。土耳其不同地区最常用的分类群是山茱萸(Cornus mas)、石竹(Dianthus zonatus var. zonatus)和黑山茱萸(Hyocyamus niger)。这些植物含有止痛和抗炎的化合物。这些植物通常作为提取物(输液/煎剂)外用，也可直接使用。然而，少数品种也用于外部。
{"title":"Plants used as painkillers in folk medicine in Turkey IV – TOOTHACHE","authors":"Meryem Seyda Erbay, S. Anıl, G. Melikoğlu","doi":"10.5152/ISTANBULJPHARM.2018.412321","DOIUrl":"https://doi.org/10.5152/ISTANBULJPHARM.2018.412321","url":null,"abstract":"DOI : 10.5152/IstanbulJPharm.2018.412321 Turkey has a rich plant diversity due to its geography and many of these plants are used in the treatment of various diseases. The usage of these medicinal plants has been passed down from generation to generation. Ethnobotanical studies are used to record traditional treatment methods and this information is intended to contribute to drug development studies. This study (which is the fourth article in a series focusing on plants with painkilling properties) details the plants used to combat toothache. In this survey, which was prepared by screening ethnobotanical research, a total of 52 taxa were traditionally used in toothache treatment in Turkey. Scientific and local names, families, parts used, and treatment methods of these plants were recorded. According to the results of the research, the most common families are Lamiaceae, Asteraceae, Solanaceae, Euphorbiaceae, Liliaceae and Rosaceae. The most commonly used taxa in different regions of Turkey are Cornus mas, Dianthus zonatus var. zonatus and Hyocyamus niger. These plants contain analgesic and anti-inflammatory compounds. These plants are usually used externally as extracts (infusion/decoction) as well as are used directly. However, a few species are also used externally.","PeriodicalId":14484,"journal":{"name":"İstanbul Journal of Pharmacy","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89738424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-09DOI: 10.5152/ISTANBULJPHARM.2018.424278
Gizem Otlatıcı, Gizem Yeğen, S. Güngör, B. Aksu
DOI : 10.5152/IstanbulJPharm.2018.424278 Skin aging is an inevitable process and the highest contributing factor to this process is the effect of ultraviolet rays. However, nutrients, dry skin, smoking, hormones and gravity are also other important factors. Skin aging is classified as intrinsic (chronological) and extrinsic (photo-aging). Generally, in aged skin, effects such as thinning, loss of elasticity, spotting, wrinkles and sagging are observed. There are various approaches to preventing skin aging. The most widely used age combating substances in cosmeceuticals are kinetin, retinoids, sun filters, herbal ingredients (such as resveratrol, turmeric, and green tea), and antioxidants (such as alpha-tocopherol, ascorbic acid, coenzyme Q10 and lipoic acid). In recent years, these molecules have been formulated as nanosized carriers such as vesicular systems, polymeric and lipid nanoparticles, nanoemulsions, dendrimers, and fullerenes. Novel carrier systems have a lot of advantages when compared to conventional formulations. In this review, an overview of cosmetic, product-oriented solutions for skin aging is given and different approaches to combat aging are summarized.
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