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The Ameliorative Impact of Cichorium intybus L. Distillate on Reproductive Parameters in Male Mice. Cichorium intybus L. Distillate 对雄性小鼠生殖参数的改善作用
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2023.96653.2826
Shima Fakher, Saeid Karbalay-Doust, Ali Noorafshan, Atefeh Seghatoleslam

Background: Cichorium intybus L. (Kasni) distillate is widely used in Eastern countries as a safe herbal drink to improve male fertility. However, the potential effects on fertility parameters and possible adverse effects have not been studied experimentally. The current study aims to evaluate the impact of Cichorium intybus L. distillate (CD) on male mice fertility.

Methods: In the present study (Shiraz, Iran), 30 male mice (30-35 g) were divided into three groups. 10 mice received distilled water (DW) for five weeks as the control group. Another 10 mice, named group CD1/2, received chicory distillate of 1/2 dilution, and the other 10 mice received chicory distillate of CD1/4 dilution as CD1/4 group, ad libitum for three weeks, and they received DW for two weeks afterward. Experimental mice were sacrificed on day 35, and sperm analysis and sera collection were performed for further investigation of FSH, LH, testosterone, and some liver and kidney function parameters. We used the left testis for stereological analysis, and the right one was excised to investigate the expression of the androgen receptor gene. For statistical analysis using SPSS 18.0, mean±SD values were analyzed by one-way analysis of variance (ANOVA) with Dunnett's analysis as post hoc to compare between groups. In stereological investigations, the Kruskal-Wallis method was used for pairwise comparisons to compare groups. The P value was considered statistically significant at P<0.05.

Results: Treatment with CD1/2 resulted in the elevation of serum FSH (P=0.002), LH (P=0.009), testosterone (P=0.034), seminiferous tubule epithelium volume (P=0.029) and length (P=0.028), and Leydig cells number (P=0.009) in comparison with the control group. Administrating CD1/2 (P=0.038) and CD1/4 (P=0.013) significantly increased sperm count compared to the control group.

Conclusion: The results revealed that using chicory distillate can improve hormone levels and sperm count in male mice.

背景:Cichorium intybus L. (Kasni)蒸馏物在东方国家被广泛用作提高男性生育能力的安全草药饮品。然而,对生育力参数的潜在影响和可能的不良反应尚未进行实验研究。本研究旨在评估 Cichorium intybus L. 蒸馏液(CD)对雄性小鼠生育能力的影响:在本研究中(伊朗设拉子),30 只雄性小鼠(30-35 克)被分为三组。10 只小鼠接受蒸馏水(DW)治疗 5 周,作为对照组。另外 10 只小鼠(CD1/2 组)接受菊苣蒸馏水 1/2 稀释液,另外 10 只小鼠接受菊苣蒸馏水 CD1/4 稀释液(CD1/4 组),自由饮用 3 周,之后接受蒸馏水 2 周。实验小鼠在第 35 天被处死,并进行精子分析和血清采集,以进一步检测 FSH、LH、睾酮和一些肝肾功能指标。我们使用左侧睾丸进行立体分析,切除右侧睾丸以研究雄激素受体基因的表达。统计分析采用 SPSS 18.0,均值±SD 值采用单因素方差分析(ANOVA),组间比较采用 Dunnett 后分析。在立体学研究中,采用 Kruskal-Wallis 方法进行成对比较,以比较各组。结果的 P 值被认为具有统计学意义:与对照组相比,CD1/2治疗可使血清FSH(P=0.002)、LH(P=0.009)、睾酮(P=0.034)、曲细精管上皮细胞体积(P=0.029)和长度(P=0.028)以及Leydig细胞数量(P=0.009)升高。与对照组相比,服用CD1/2(P=0.038)和CD1/4(P=0.013)可显著增加精子数量:结果表明,使用菊苣蒸馏物可以改善雄性小鼠的激素水平和精子数量。
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引用次数: 0
Association of Insomnia with Anxiety and Depression in Type 2 Diabetic Patients: A Cross-Sectional Study. 2型糖尿病患者失眠与焦虑和抑郁的关系:一项横断面研究。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/ijms.2023.96017.2755
Karlyghash Shinalieva, Asemgul Kasenova, Zauresh Akhmetzhanova, Dinara Alzhanova, Laura Eszhanova, Ainur Bekenova

Background: Type 2 diabetes mellitus (T2DM) is associated with sleep disorders, which in turn may lead to anxiety and depression. Sleep deprivation impairs glucose metabolism causing a decrease in insulin sensitivity and glucose tolerance. The present study aimed to determine the association of insomnia with anxiety and depression in patients with T2DM.

Methods: A cross-sectional study was conducted in 2020 at the Endocrinological Department of City Clinical Hospital №1, affiliated with Astana Medical University (Astana, Kazakhstan). A total of 376 patients with decompensated T2DM were included in the study. Insomnia was assessed using the Pittsburgh Sleep Quality Index (PSQI) and Insomnia Severity Index (ISI). Anxiety and depression were assessed using the Hospital Anxiety and Depression Scale (HADS). Multivariable logistic regression was used to assess the association of insomnia with anxiety and depression.

Results: All patients showed signs of sleep disturbances. Based on the HADS cutoff score >8, anxiety and depression were observed in 48 (12.80%) and 46 (12.20%) patients, respectively. PSQI, ISI, and HADS indicators were associated with an increased risk of anxiety and depression symptoms. In terms of anxiety, the adjusted odds ratio for PSQI and ISI scores were 1.09 (P=0.08) and 1.07 (P=0.01), respectively. These for depression were 1.10 (P=0.06) and 1.07 (P=0.01), respectively.

Conclusion: Sleep quality is an important indicator of psychological health in patients with T2DM, especially those who exhibit signs of anxiety and depression.

背景:2型糖尿病(T2DM)与睡眠障碍有关,而睡眠障碍又可能导致焦虑和抑郁。睡眠不足会损害葡萄糖代谢,导致胰岛素敏感性和糖耐量下降。本研究旨在确定T2DM患者失眠与焦虑和抑郁的关系。方法:2020年在城市临床医院内分泌科进行了一项横断面研究№1,隶属于阿斯塔纳医科大学(哈萨克斯坦阿斯塔纳)。本研究共纳入376例失代偿期T2DM患者。使用匹兹堡睡眠质量指数(PSQI)和失眠严重程度指数(ISI)评估失眠。使用医院焦虑和抑郁量表(HADS)评估焦虑和抑郁。多变量逻辑回归用于评估失眠与焦虑和抑郁的关系。结果:所有患者均出现睡眠障碍症状。基于HADS临界分数>8,分别在48名(12.80%)和46名(12.20%)患者中观察到焦虑和抑郁。PSQI、ISI和HADS指标与焦虑和抑郁症状的风险增加有关。在焦虑方面,PSQI和ISI评分的调整比值比分别为1.09(P=0.08)和1.07(P=0.01)。抑郁症的发病率分别为1.10(P=0.06)和1.07(P=0.01)。结论:睡眠质量是2型糖尿病患者心理健康的重要指标,尤其是那些表现出焦虑和抑郁症状的患者。
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引用次数: 0
Mechanical Ablation of the Posterior Urethral Valve Omitting Thermal Energy: A Brief Report. 省略热能的后尿道瓣膜机械消融:一份简要报告。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/IJMS.2022.95313.2660
Farzaneh Sharifiaghdas, Narjes Saberi

Management of the posterior urethral valve (PUV) is a clinical challenge in pediatric urology. We report the results of a modified valve ablation method without using a pediatric resectoscope and thermal energy. Patients were selected from children with PUV who were referred to the pediatric urology clinic of Shahid Labbafinejad Hospital, Tehran, Iran, and have undergone endoscopic valve ablation surgery between May 2019 to May 2021. Ten male patients with PUV underwent mechanical valve ablation without the use of the conventional pediatric resectoscope, and thermal energy was replaced by a 6F semi-rigid urethroscope and 3Fr ureteral catheter. Patients were assessed both pre-and postoperatively using serum creatinine, urinary tract ultrasound imaging, and voiding cystourethrography. The mean age was 23.88±30.13 months (range= 25 days to 8 years). Four out of 10 patients (40%) had elevated serum creatinine, and seven had unilateral or bilateral hydroureteronephrosis (70%). No major complications were reported according to Clavien-Dindo Classification System. The level of serum creatinine, the grade of hydroureteronephrosis, and the ratio of the prostatic urethra to anterior urethra diameter in postoperative voiding cystourethrography were decreased. A decrease in serum creatinine level occurred in patients after valve ablation, but this decrease was not statistically significant (P=0.059). The decrease in hydroureteronephrosis grade on the right (P=0.006) and left (P=0.022) was statistically significant. There was no evidence of urethral stenosis or need for repeating resection. It can be concluded that our mechanical valve ablation method might be a safe and effective technique for PUV ablation.

后尿道瓣膜(PUV)的管理是儿科泌尿外科的一个临床挑战。我们报告了一种改良的瓣膜消融方法的结果,该方法不使用儿科切除镜和热能。患者选自PUV儿童,这些儿童被转诊至伊朗德黑兰Shahid Labbafinejad医院的儿科泌尿外科诊所,并在2019年5月至2021年5月期间接受了内窥镜瓣膜消融手术。10名男性PUV患者在不使用传统儿科切除镜的情况下进行了机械瓣膜消融,并用6F半刚性尿道镜和3Fr输尿管导管代替热能。患者在术前和术后均使用血清肌酐、尿路超声成像和排尿膀胱尿道造影进行评估。平均年龄为23.88±30.13个月(25天至8岁)。10名患者中有4名(40%)血清肌酐升高,7名患者患有单侧或双侧输尿管积水性肾病(70%)。根据Clavien-Dindo分类系统,未报告重大并发症。术后排尿膀胱尿道造影中血清肌酐水平、输尿管水性肾病分级以及前列腺尿道与前尿道直径的比值均降低。瓣膜消融术后患者血清肌酸酐水平下降,但这种下降没有统计学意义(P=0.059)。右侧(P=0.006)和左侧(P=0.022)的输尿管积水性肾病分级下降具有统计学意义。没有证据表明尿道狭窄或需要再次切除。可以得出结论,我们的机械瓣膜消融方法可能是一种安全有效的PUV消融技术。
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引用次数: 0
Human Herpesvirus Detection in Removable Denture Wearers' Saliva Compared to Dentate Cases. 可摘义齿佩戴者唾液中人类疱疹病毒的检测与义齿病例的比较。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/ijms.2023.97293.2911
Hanieh Farahmand, Kiana Agha Sadeghi, Afagh Moattari, Mitra Farzin, Hamed Rakhshbahar, Soheil Esmaeili, Armin Farajollahi
In this study, 100 saliva samples, including 57 (57%) men and 43 (43%) women, were examined. The mean age of the case group was 71.6±10.2 years, and the mean age of the control group was 67.3±15.3 years. All the case and control groups were matched. Table 1 shows the viruses that were found in the case and control groups. In the dentate subjects, no HSV-1 and HSV-2, CMV and EBV, and HHV-6 were detected. However, 4 (8%) of the case group were infected with HSV-1, and 1 (2%) of them were infected with HSV-2. Moreover, 1 (2%) of the case group had EBV infection, 2 (4%) had CMV infection, and 2 (4%) had HHV-6 infection. There were no underlying diseases in the control group. There was no statistically significant correlation between underlying diseases such as hypertension and diabetes mellitus and HSV-1 and HSV-2 prevalence (P=0.412). Furthermore, no statistically significant correlation was found between the underlying diseases and EBV, CMV, and HHV-6 infection (P=0.406). HSV-1, HSV-2, HHV-6, EBV, and CMV were detected in the saliva of denture wearers. The Chi square test indicated a higher and more significant HSV-1 value among denture wearers. For the other viruses, however, there was no significant difference between case and control groups. A previous study found HHV-8 in denture wearers’ saliva, 5 similar to what we detected regarding HSV-1. Except for HSV-1, the findings of the present study indicated no significant difference between the case and control groups. Therefore, further studies with larger sample sizes are recommended. This study could pave the way for further research regarding the significance and risk of these viruses in edentulous cases.
{"title":"Human Herpesvirus Detection in Removable Denture Wearers' Saliva Compared to Dentate Cases.","authors":"Hanieh Farahmand,&nbsp;Kiana Agha Sadeghi,&nbsp;Afagh Moattari,&nbsp;Mitra Farzin,&nbsp;Hamed Rakhshbahar,&nbsp;Soheil Esmaeili,&nbsp;Armin Farajollahi","doi":"10.30476/ijms.2023.97293.2911","DOIUrl":"10.30476/ijms.2023.97293.2911","url":null,"abstract":"In this study, 100 saliva samples, including 57 (57%) men and 43 (43%) women, were examined. The mean age of the case group was 71.6±10.2 years, and the mean age of the control group was 67.3±15.3 years. All the case and control groups were matched. Table 1 shows the viruses that were found in the case and control groups. In the dentate subjects, no HSV-1 and HSV-2, CMV and EBV, and HHV-6 were detected. However, 4 (8%) of the case group were infected with HSV-1, and 1 (2%) of them were infected with HSV-2. Moreover, 1 (2%) of the case group had EBV infection, 2 (4%) had CMV infection, and 2 (4%) had HHV-6 infection. There were no underlying diseases in the control group. There was no statistically significant correlation between underlying diseases such as hypertension and diabetes mellitus and HSV-1 and HSV-2 prevalence (P=0.412). Furthermore, no statistically significant correlation was found between the underlying diseases and EBV, CMV, and HHV-6 infection (P=0.406). HSV-1, HSV-2, HHV-6, EBV, and CMV were detected in the saliva of denture wearers. The Chi square test indicated a higher and more significant HSV-1 value among denture wearers. For the other viruses, however, there was no significant difference between case and control groups. A previous study found HHV-8 in denture wearers’ saliva, 5 similar to what we detected regarding HSV-1. Except for HSV-1, the findings of the present study indicated no significant difference between the case and control groups. Therefore, further studies with larger sample sizes are recommended. This study could pave the way for further research regarding the significance and risk of these viruses in edentulous cases.","PeriodicalId":14510,"journal":{"name":"Iranian Journal of Medical Sciences","volume":"48 5","pages":"522-524"},"PeriodicalIF":2.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/55/fd/IJMS-48-522.PMC10541547.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41129280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association of Cytochrome P450 2E1 and N-Acetyltransferase 2 Genotypes with Serum Isoniazid Level and Anti-Tuberculosis Drug-Induced Hepatotoxicity: A Cross-Sectional Study. 细胞色素P450 2E1和N-乙酰转移酶2基因型与血清异烟肼水平和抗结核药物诱导的肝毒性的相关性:一项横断面研究。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/ijms.2023.96145.2765
Nasir Pourmohamadi, Mihan Pour Abdollah Toutkaboni, Nasim Hayati Roodbari, Payam Tabarsi, Shadi Baniasadi

Background: Anti-tuberculosis drug-induced hepatotoxicity can result from genetic polymorphism of the isoniazid (INH) metabolizing enzyme. This study aimed to determine the effect of genetic polymorphism of N-acetyltransferase 2 (NAT2) and cytochrome P450 2E1 (CYP2E1) genes on serum isoniazid level and drug-induced hepatotoxicity.

Methods: A cross-sectional study was conducted on 120 patients (with and without hepatotoxicity) with pulmonary tuberculosis from June 2019 to April 2022 in Tehran (Iran). High-performance liquid chromatography was used to measure the serum concentration of INH and acetylisoniazid (AcINH). NAT2 and CYP2E1 genotypes were determined using polymerase chain reaction and restriction fragment length polymorphism methods. Data were analyzed using SPSS software (version 22.0) with independent two-sample t test, Chi square test, or Fisher's exact test. P<0.05 was considered statistically significant.

Results: A total of 40 patients showed hepatotoxicity. The risk of anti-tuberculosis drug-induced hepatotoxicity was significantly higher in patients who are slow acetylator (SA) phenotype than in rapid or intermediate acetylator (P<0.001). NAT2*4/*4 genotypes were not found in patients with hepatotoxicity. The frequency of NAT2*5 and NAT2*6 haplotypes and serum INH concentration was significantly higher in patients with hepatotoxicity than in those without (P=0.003, P<0.001, and P<0.001, respectively). NAT2*4 haplotype was correlated with protection against hepatotoxicity. A combination of SA and CYP2E1 C1/C1 genotype was significantly associated with hepatotoxicity (P<0.001).

Conclusion: Hepatotoxicity in Iranian patients with tuberculosis was confirmed due to the presence of NAT2 SA polymorphism. Determining NAT2 and CYP2E1 genotypes and/or INH concentration can be a valuable tool to identify patients susceptible to hepatotoxicity.

背景:抗结核药物引起的肝毒性可能是异烟肼代谢酶基因多态性的结果。本研究旨在确定N-乙酰转移酶2(NAT2)和细胞色素P4502E1(CYP2E1)基因的遗传多态性对血清异烟肼水平和药物诱导的肝毒性的影响。方法:2019年6月至2022年4月,在伊朗德黑兰对120名肺结核患者(有肝毒性和无肝毒性)进行了横断面研究。采用高效液相色谱法测定血清异烟肼和乙酰异烟肼浓度。采用聚合酶链式反应和限制性片段长度多态性方法测定NAT2和CYP2E1基因型。数据使用SPSS软件(22.0版)进行分析,采用独立双样本t检验、卡方检验或Fisher精确检验。结果:共有40例患者出现肝毒性。具有慢乙酰化(SA)表型的患者发生抗结核药物性肝毒性的风险显著高于快速或中等乙酰化(PNAT2*4/*4基因型在具有肝毒性的患者中未发现)。具有肝毒性患者的NAT2*5和NAT2*6单倍型的频率和血清INH浓度显著高于没有肝毒性患者(P=0.003,PNAT2*4单倍型与肝毒性保护相关。SA和CYP2E1 C1/C1基因型的组合与肝毒性显著相关(结论:伊朗肺结核患者的肝毒性是由于NAT2-SA多态性的存在而被证实的。测定NAT2和CYP2E1基因型和/或INH浓度可以成为识别易感肝毒性患者的有价值的工具。
{"title":"Association of Cytochrome P450 2E1 and N-Acetyltransferase 2 Genotypes with Serum Isoniazid Level and Anti-Tuberculosis Drug-Induced Hepatotoxicity: A Cross-Sectional Study.","authors":"Nasir Pourmohamadi,&nbsp;Mihan Pour Abdollah Toutkaboni,&nbsp;Nasim Hayati Roodbari,&nbsp;Payam Tabarsi,&nbsp;Shadi Baniasadi","doi":"10.30476/ijms.2023.96145.2765","DOIUrl":"10.30476/ijms.2023.96145.2765","url":null,"abstract":"<p><strong>Background: </strong>Anti-tuberculosis drug-induced hepatotoxicity can result from genetic polymorphism of the isoniazid (INH) metabolizing enzyme. This study aimed to determine the effect of genetic polymorphism of N-acetyltransferase 2 (<i>NAT2</i>) and cytochrome P450 2E1 (<i>CYP2E1</i>) genes on serum isoniazid level and drug-induced hepatotoxicity.</p><p><strong>Methods: </strong>A cross-sectional study was conducted on 120 patients (with and without hepatotoxicity) with pulmonary tuberculosis from June 2019 to April 2022 in Tehran (Iran). High-performance liquid chromatography was used to measure the serum concentration of INH and acetylisoniazid (AcINH). <i>NAT2</i> and <i>CYP2E1</i> genotypes were determined using polymerase chain reaction and restriction fragment length polymorphism methods. Data were analyzed using SPSS software (version 22.0) with independent two-sample <i>t</i> test, Chi square test, or Fisher's exac<i>t</i> test. P<0.05 was considered statistically significant.</p><p><strong>Results: </strong>A total of 40 patients showed hepatotoxicity. The risk of anti-tuberculosis drug-induced hepatotoxicity was significantly higher in patients who are slow acetylator (SA) phenotype than in rapid or intermediate acetylator (P<0.001). <i>NAT2</i>*4/*4 genotypes were not found in patients with hepatotoxicity. The frequency of <i>NAT2</i>*5 and <i>NAT2</i>*6 haplotypes and serum INH concentration was significantly higher in patients with hepatotoxicity than in those without (P=0.003, P<0.001, and P<0.001, respectively). <i>NAT2</i>*4 haplotype was correlated with protection against hepatotoxicity. A combination of SA and <i>CYP2E1</i> C1/C1 genotype was significantly associated with hepatotoxicity (P<0.001).</p><p><strong>Conclusion: </strong>Hepatotoxicity in Iranian patients with tuberculosis was confirmed due to the presence of <i>NAT2</i> SA polymorphism. Determining <i>NAT2</i> and <i>CYP2E1</i> genotypes and/or INH concentration can be a valuable tool to identify patients susceptible to hepatotoxicity.</p>","PeriodicalId":14510,"journal":{"name":"Iranian Journal of Medical Sciences","volume":"48 5","pages":"474-483"},"PeriodicalIF":2.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/aa/b6/IJMS-48-474.PMC10541540.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41144097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Association between Metabolic Syndrome Risk Factors and Immunohistochemical Profile in Women with Breast Cancer. 癌症妇女代谢综合征危险因素与免疫组织化学特征的相关性。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/IJMS.2022.95039.2673
Hiva Danesh, Robab Anbiaei, Nasrin Ziamajidi, Maryam Farhadian, Zeinab Barartabar, Roghayeh Abbasalipourkabir

Background: The association between metabolic syndrome (MetS) and breast cancer may significantly impact the mortality and incidence of breast cancer. This study aimed to assess the association between MetS risk factors and immunohistochemical (IHC) profiles in women with breast cancer.

Methods: This cross-sectional study used the medical records of 300 breast cancer patients with an average age of 53.11±12.97 years in the Chemotherapy and Radiation Therapy Clinic of Dr. Anbiai, Tehran, Iran (2020-2021). The cases were divided into five subgroups including luminal A, luminal B (HER-2-), luminal B (HER-2+), HER-2 overexpressing, and triple negative.

Results: There was no difference in the prognostic indicators between the presence and absence of MetS in women with breast cancer. A higher proportion of luminal A tumors (39.3%), luminal B (HER-2+) (25%), triple-negative (17%), luminal B (HER-2-) (10.7%), HER-2 overexpression (8%) was observed in women with MetS than those without MetS. Multivariate logistic regression analysis showed that patients with MetS had a 41% higher chance of developing luminal A than those without MetS, and patients with a BMI≥30 Kg/m2 had an 80% higher chance of developing luminal B (HER-2+) than those with a BMI<30 Kg/m2. Moreover, women with a waist circumference higher than 88 cm had a 14 % lower chance of developing Luminal B (HER-2+) than those with a waist circumference less than 88 cm.

Conclusion: There was no difference in prognostic indicators and IHC profile in patients with and without MetS.

背景:代谢综合征(MetS)与乳腺癌症之间的相关性可能显著影响癌症的死亡率和发病率。本研究旨在评估癌症妇女的MetS危险因素与免疫组织化学(IHC)谱之间的关系。方法:本横断面研究使用了伊朗德黑兰Anbiai医生化疗和放射治疗诊所(2020-2021年)300名平均年龄为53.11±12.97岁的癌症患者的医疗记录。将这些病例分为五个亚组,包括管腔A、管腔B(HER-2-)、腔B(HER-2+)、HER-2过表达和三阴性。结果:癌症妇女有无MetS的预后指标没有差异。在患有MetS的女性中,观察到管腔A肿瘤(39.3%)、管腔B肿瘤(HER-2+)(25%)、三阴性(17%)、管腔B肿瘤(HER-2-)(10.7%)和HER-2过表达(8%)的比例高于未患有MetS的女性。多因素logistic回归分析显示,患有代谢综合征的患者发生管腔a的几率比没有代谢综合征患者高41%,BMI≥30 Kg/m2的患者患管腔B(HER-2+)的几率比患有BMI2的患者高80%。此外,腰围大于88 cm的女性患Luminal B(HER-2+)的几率比腰围小于88 cm的患者低14%。结论:MetS患者和非MetS患者的预后指标和IHC特征没有差异。
{"title":"Association between Metabolic Syndrome Risk Factors and Immunohistochemical Profile in Women with Breast Cancer.","authors":"Hiva Danesh,&nbsp;Robab Anbiaei,&nbsp;Nasrin Ziamajidi,&nbsp;Maryam Farhadian,&nbsp;Zeinab Barartabar,&nbsp;Roghayeh Abbasalipourkabir","doi":"10.30476/IJMS.2022.95039.2673","DOIUrl":"10.30476/IJMS.2022.95039.2673","url":null,"abstract":"<p><strong>Background: </strong>The association between metabolic syndrome (MetS) and breast cancer may significantly impact the mortality and incidence of breast cancer. This study aimed to assess the association between MetS risk factors and immunohistochemical (IHC) profiles in women with breast cancer.</p><p><strong>Methods: </strong>This cross-sectional study used the medical records of 300 breast cancer patients with an average age of 53.11±12.97 years in the Chemotherapy and Radiation Therapy Clinic of Dr. Anbiai, Tehran, Iran (2020-2021). The cases were divided into five subgroups including luminal A, luminal B (HER-2<sup>-</sup>), luminal B (HER-2<sup>+</sup>), HER-2 overexpressing, and triple negative.</p><p><strong>Results: </strong>There was no difference in the prognostic indicators between the presence and absence of MetS in women with breast cancer. A higher proportion of luminal A tumors (39.3%), luminal B (HER-2<sup>+</sup>) (25%), triple-negative (17%), luminal B (HER-2<sup>-</sup>) (10.7%), HER-2 overexpression (8%) was observed in women with MetS than those without MetS. Multivariate logistic regression analysis showed that patients with MetS had a 41% higher chance of developing luminal A than those without MetS, and patients with a BMI≥30 Kg/m<sup>2</sup> had an 80% higher chance of developing luminal B (HER-2<sup>+</sup>) than those with a BMI<30 Kg/m<sup>2</sup>. Moreover, women with a waist circumference higher than 88 cm had a 14 % lower chance of developing Luminal B (HER-2<sup>+</sup>) than those with a waist circumference less than 88 cm.</p><p><strong>Conclusion: </strong>There was no difference in prognostic indicators and IHC profile in patients with and without MetS.</p>","PeriodicalId":14510,"journal":{"name":"Iranian Journal of Medical Sciences","volume":"48 5","pages":"456-464"},"PeriodicalIF":2.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8a/43/IJMS-48-456.PMC10541543.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41139633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Topical Licorice for Aphthous: A Systematic Review of Clinical Trials. 局部甘草治疗阿弗他病:临床试验的系统综述。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/IJMS.2022.94467.2576
Fereshteh Dorsareh, Gita Vahid-Dastjerdi, Abdelhakim Bouyahya, Mohammad Mehdi Zarshenas, Mostafa Rezaie, Woong Mo Yang, Ehsan Amiri-Ardekani

Background: Recurrent aphthous stomatitis (RAS) is the most common ulcerative disease that affects oral mucosa. The coating agents, topical analgesics, and topical steroids are usually used as treatment methods. Glycyrrhiza glabra has been used for RAS treatment based on its anti-inflammatory, antioxidant, and immunomodulatory properties. In this study, a systemic review on the therapeutic effect of topical licorice on RAS management was performed.

Methods: Science Direct, Scopus, Cochrane databases, PubMed Google Scholar, and ResearchGate were searched up to September 2021 to find all English randomized clinical trials studying the effect of G. glabra, or its compositions on RAS. Meta-analysis was not conducted because of data heterogeneity. Articles were reviewed qualitatively, and only those with a Jadad score ≥3 were included. Animal studies, in vitro, review papers, non-English papers, and case reports were excluded.

Results: Six studies with 314 subjects were included after screening. The result showed licorice has significant effects on RAS pain reduction, ulcer size, and healing time. Its effectiveness is related to its dose-dependent anti-inflammatory and antioxidant effects through several mechanisms. It also has antibacterial effects against Streptococci mutans and Porphyromonas gingivalis as another mechanism of action in RAS treatment. In addition, licorice can elevate the epidermal growth factor (EGF) level compared to the control group, which has an essential role in oral mucosal tissue integrity.

Conclusion: Licorice extract has been used in different dosage forms, including paste, patch, and mouthwash with concentrations of 1% or 5%. The healing time after licorice therapy is expected to be within 4-8 days. Licorice did not show any adverse effect in the intervention groups, indicating its effectiveness and safety in RAS treatment.

背景:复发性口腔炎(RAS)是影响口腔黏膜的最常见的溃疡性疾病。通常使用包衣剂、局部镇痛剂和局部类固醇作为治疗方法。光甘草因其抗炎、抗氧化和免疫调节特性而被用于RAS治疗。在本研究中,对局部甘草对RAS管理的治疗效果进行了系统综述。方法:检索截至2021年9月的Science Direct、Scopus、Cochrane数据库、PubMed Google Scholar和ResearchGate,以查找所有研究G.glabra或其组合物对RAS影响的英国随机临床试验。由于数据的异质性,没有进行荟萃分析。对文章进行了定性审查,仅包括Jadad评分≥3的文章。排除动物研究、体外研究、综述论文、非英文论文和病例报告。结果:筛选后纳入6项研究,314名受试者。结果表明,甘草对RAS疼痛减轻、溃疡大小和愈合时间有显著影响。其有效性通过多种机制与剂量依赖性抗炎和抗氧化作用有关。它还对变形链球菌和牙龈卟啉单胞菌具有抗菌作用,这是RAS治疗的另一种作用机制。此外,与对照组相比,甘草可以提高表皮生长因子(EGF)水平,这对口腔粘膜组织的完整性具有重要作用。结论:甘草提取物有不同的剂型,包括浓度为1%或5%的糊剂、贴片和漱口水。甘草治疗后的愈合时间预计在4-8天内。甘草在干预组中没有表现出任何不良反应,表明其在RAS治疗中的有效性和安全性。
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引用次数: 1
Lipid Profile as a Predictive Marker for Organ Dysfunction after Thoracoabdominal Surgery: A Cross-sectional Study. 脂质谱作为胸腹手术后器官功能障碍的预测标志物:一项横断面研究。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/IJMS.2022.95364.2672
Sarvin Sanaie, Sanam Dolati, Majid Montazer, Sarina Ranjbari, Arezoo Fathalizadeh, Kamran Shadvar, Elnaz Faramarzi, Ata Mahmoodpoor

Background: Plasma total cholesterol is considered a negative acute phase reactant. In various pathological conditions, such as trauma, sepsis, burns, and liver dysfunction, as well as post-surgery, serum cholesterol level decreases. This study aimed to investigate the role of lipid profiles in determining the probability of organ dysfunction after surgery.

Methods: This cross-sectional study included patients who underwent thoracoabdominal surgery and were admitted to the intensive care unit of Imam Reza Hospital in Tabriz, Iran, between October 2016 and September 2018. During the first two days of admission, blood samples were taken, and serum levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), Low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), and albumin were measured. The relation between the changes in these laboratory markers and six organ functions including cardiovascular, respiratory, renal, central nervous system, hepatic, and hematologic, length of stay in the hospital and intensive care unit, mechanical ventilation duration, and vasopressor use were investigated. The independent t test was used to compare continuous variables. The association between different variables and organ dysfunction and mortality was evaluated by using logistic regression.

Results: The serum TC increased the risk of mortality (OR=1.09, 95%CI=1.06-1.11, P<0.001), renal dysfunction (OR=1.09, 95%CI=1.06-1.12; P<0.001), liver dysfunction (OR=1.07, 95%CI=1.03-1.10; P<0.001), respiratory dysfunction (OR=1.08, 95%CI=1.05-1.13; P<0.001). Moreover, LDL, HDL, and TG were found to be inversely related to mortality, organ dysfunction, length of stay in the hospital and intensive care unit, mechanical ventilation duration, and vasopressor use.

Conclusion: TC could be considered a risk factor for mortality, organ dysfunction, and clinical outcomes. On the other hand, LDL, HDL, and TG played a protective role in the patients' mortality, organ dysfunction, and clinical outcomes.

背景:血浆总胆固醇被认为是一种负急性期反应物。在各种病理条件下,如创伤、败血症、烧伤、肝功能障碍以及手术后,血清胆固醇水平下降。本研究旨在探讨脂质谱在确定手术后器官功能障碍概率中的作用。方法:这项横断面研究包括2016年10月至2018年9月期间接受胸腹手术并入住伊朗大不里士伊玛目礼萨医院重症监护室的患者。在入院的前两天,采集血样,测量血清总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白蛋白胆固醇(LDL-C)、甘油三酯(TG)和白蛋白水平。研究了这些实验室标志物的变化与六种器官功能之间的关系,包括心血管、呼吸、肾脏、中枢神经系统、肝脏和血液学、在医院和重症监护室的住院时间、机械通气持续时间和血管升压药的使用。独立t检验用于比较连续变量。采用逻辑回归法评估不同变量与器官功能障碍和死亡率之间的相关性。结果:血清TC增加了患者的死亡率(OR=1.09,95%CI=1.06-11.11,P结论:TC可被认为是死亡率、器官功能障碍和临床结果的危险因素。另一方面,LDL、HDL和TG对患者的死亡率、器官功能不全和临床结果起到保护作用。
{"title":"Lipid Profile as a Predictive Marker for Organ Dysfunction after Thoracoabdominal Surgery: A Cross-sectional Study.","authors":"Sarvin Sanaie,&nbsp;Sanam Dolati,&nbsp;Majid Montazer,&nbsp;Sarina Ranjbari,&nbsp;Arezoo Fathalizadeh,&nbsp;Kamran Shadvar,&nbsp;Elnaz Faramarzi,&nbsp;Ata Mahmoodpoor","doi":"10.30476/IJMS.2022.95364.2672","DOIUrl":"10.30476/IJMS.2022.95364.2672","url":null,"abstract":"<p><strong>Background: </strong>Plasma total cholesterol is considered a negative acute phase reactant. In various pathological conditions, such as trauma, sepsis, burns, and liver dysfunction, as well as post-surgery, serum cholesterol level decreases. This study aimed to investigate the role of lipid profiles in determining the probability of organ dysfunction after surgery.</p><p><strong>Methods: </strong>This cross-sectional study included patients who underwent thoracoabdominal surgery and were admitted to the intensive care unit of Imam Reza Hospital in Tabriz, Iran, between October 2016 and September 2018. During the first two days of admission, blood samples were taken, and serum levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), Low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), and albumin were measured. The relation between the changes in these laboratory markers and six organ functions including cardiovascular, respiratory, renal, central nervous system, hepatic, and hematologic, length of stay in the hospital and intensive care unit, mechanical ventilation duration, and vasopressor use were investigated. The independent <i>t</i> test was used to compare continuous variables. The association between different variables and organ dysfunction and mortality was evaluated by using logistic regression.</p><p><strong>Results: </strong>The serum TC increased the risk of mortality (OR=1.09, 95%CI=1.06-1.11, P<0.001), renal dysfunction (OR=1.09, 95%CI=1.06-1.12; P<0.001), liver dysfunction (OR=1.07, 95%CI=1.03-1.10; P<0.001), respiratory dysfunction (OR=1.08, 95%CI=1.05-1.13; P<0.001). Moreover, LDL, HDL, and TG were found to be inversely related to mortality, organ dysfunction, length of stay in the hospital and intensive care unit, mechanical ventilation duration, and vasopressor use.</p><p><strong>Conclusion: </strong>TC could be considered a risk factor for mortality, organ dysfunction, and clinical outcomes. On the other hand, LDL, HDL, and TG played a protective role in the patients' mortality, organ dysfunction, and clinical outcomes.</p>","PeriodicalId":14510,"journal":{"name":"Iranian Journal of Medical Sciences","volume":"48 5","pages":"465-473"},"PeriodicalIF":2.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2a/7d/IJMS-48-465.PMC10541545.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41144205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective Effect of Docetaxel Against Autophagy-Related Genes in Vitrification of Mouse Metaphase II Oocytes. 多西他赛对小鼠II期卵母细胞玻璃化过程中自噬相关基因的保护作用。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/IJMS.2023.88390.2811
Hamed Daneshpazhouh, Nasim Hayati Roodbari, Yaser Tahamtani, Zahra Khodabandeh, Mehdi Dianatpour

Background: Autophagy is a conservative mechanism for cell survival as the main response of cells to stress conditions. The present study aimed to assess the effect of docetaxel on the survival, fertilization, and expression of autophagy-related genes in vitrified oocytes.

Methods: The study was conducted in 2018 at the Stem Cells Technology Research Center, Shiraz University of Medical Sciences (Shiraz, Iran). Denuded oocytes were randomly selected and assigned to five groups, namely control (n=133), docetaxel (n=136), docetaxel+cryoprotectants (n=146), docetaxel+vitrification (n=138), and vitrification (n=145). The effect of vitrification on the expression of autophagy-related gene 5 (ATG5) and Beclin-1 was determined using a real-time polymerase chain reaction. Data were analyzed using SPSS software (version 26.0) and GraphPad Prism 9.

Results: Survival and fertilization rates in each experimental group were significantly reduced compared to the control group (P=0.001). After in vitro fertilization of oocytes, the 2-cell formation rate was significantly reduced in the docetaxel+vitrification and vitrification groups compared to the control and docetaxel groups (P=0.001 and P=0.001, respectively). Pre-incubation of oocytes with docetaxel reduced gene expression levels of Beclin-1 and ATG5 in the docetaxel+cryoprotectants and docetaxel+vitrification groups (P=0.001 and P=0.019, respectively). The expression level of these genes was also reduced in the docetaxel group compared to the control group (P=0.001).

Conclusion: Incubation of mouse metaphase II oocytes with docetaxel prior to vitrification reduced the expression of autophagy-related genes and increased survival and fertilization rates compared to untreated oocytes.

背景:自噬是细胞生存的一种保守机制,是细胞对应激条件的主要反应。本研究旨在评估多西他赛对玻璃化卵母细胞存活、受精和自噬相关基因表达的影响。方法:该研究于2018年在伊朗设拉子医学科学大学干细胞技术研究中心进行。将变性的卵母细胞随机选择并分为五组,即对照组(n=133)、多西他赛组(n=136)、多西他赛+冷冻保护剂组(n=146)、多西他赛+玻璃化处理组(n=138)和玻璃化处理(n=145)。玻璃化处理对自噬相关基因5(ATG5)和Beclin-1表达的影响通过实时聚合酶链反应测定。使用SPSS软件(版本26.0)和GraphPad Prism 9对数据进行分析。结果:与对照组相比,各实验组的存活率和受精率均显著降低(P=0.001)。卵母细胞体外受精后,多西他赛+玻璃化和玻璃化组中的2-细胞形成率与对照组和多西他塞尔组相比显著降低(分别为P=0.001和P=0.001)。在多西他赛+冷冻保护剂和多西他塞尔+玻璃化组中,用多西他塞预孵育卵母细胞降低了Beclin-1和ATG5的基因表达水平(分别为P=0.001和P=0.019)。与对照组相比,多西他赛组的这些基因表达水平也降低了(P=0.001)。结论:与未处理的卵母细胞相比,玻璃化前用多西他塞尔孵育小鼠中期II卵母细胞降低了自噬相关基因的表达,并提高了存活率和受精率。
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引用次数: 0
Development of a Modified Friedewald's Formula to Calculate Low-Density Lipoprotein in an Iranian Population. 计算伊朗人群低密度脂蛋白的改良Friedewald公式的发展。
IF 2.4 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-09-01 DOI: 10.30476/IJMS.2022.95469.2683
Seyed Alireza Sobhani, Masoumeh Kheirandish, Shideh Rafati, Milad Rafat, Roghayeh Shahbazi, Abnoos Azarbad, Masoumeh Mahmoodi, Ebrahim Eftekhar, Somayeh Kheirandish

Background: Elevated low-density lipoprotein cholesterol (LDL-C) is a significant risk factor for cardiovascular diseases. LDL-C can be directly measured using various methods, but this requires expensive equipment. Currently, clinical laboratories estimate LDL-C based on Friedewald's formula (FF). We aimed to develop a modified formula based on directly measured LDL-C (D-LDL-C) values in a large population in Southern Iran and compare the results with various other estimation formulas.

Methods: The participants of this cross-sectional study were adults aged >18 years living in Southern Iran. Blood samples from 15,200 individuals were collected, and the measured lipid parameters were randomly divided into training (n=10,184) and validation (n=5,016) datasets. A new formula was developed using a linear regression model, and its accuracy was validated. Pearson's correlation and Cohen's kappa were used to determin the relationship between D-LDL-C and calculated LDL-C (C-LDL-C).

Results: The developed formula for the estimation of LDL-C was 0.857 total cholesterol (TC)-0.915 high-density lipoprotein cholesterol (HDL-C)-0.115 triglycerides (TG). Based on our proposed formula, for TG<150 and TG≥150 mg/dL, there was a significant correlation between mean values of D-LDL-C and C-LDL-C (r=0.985 and r=0.974, respectively). Compared to other formulas, C-LDL-C obtained from the proposed formula had the highest correlation with D-LDL-C. The agreement between D-LDL-C and C-LDL-C for TC<200, 200-239, and ≥240 mg/dL was 80.8%, 63.2%, and 67.4%, respectively, indicating a higher level of agreement than other formulas.

Conclusion: The new formula appears to be more accurate than FF when applied to the population of Southern Iran.

背景:低密度脂蛋白胆固醇(LDL-C)升高是心血管疾病的重要危险因素。LDL-C可以使用各种方法直接测量,但这需要昂贵的设备。目前,临床实验室根据弗里德瓦尔德公式(FF)估计LDL-C。我们的目的是基于伊朗南部大量人群中直接测量的LDL-C(D-LDL-C)值开发一个修正公式,并将结果与其他各种估计公式进行比较。方法:这项横断面研究的参与者是居住在伊朗南部的18岁以上的成年人。收集了15200名个体的血液样本,并将测量的脂质参数随机分为训练(n=10184)和验证(n=5016)数据集。利用线性回归模型建立了一个新的公式,并对其准确性进行了验证。采用Pearson相关和Cohen’s kappa法测定D-低密度脂蛋白胆固醇与计算的低密度脂素胆固醇(C-LDL-C)之间的关系。结果:LDL-C的估算公式为0.857总胆固醇(TC)-0.915高密度脂蛋白-胆固醇(HDL-C)-0.115甘油三酯(TG)。基于我们提出的公式,对于TG结论:当应用于伊朗南部人口时,新公式似乎比FF更准确。
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引用次数: 0
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Iranian Journal of Medical Sciences
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