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Comparison of the Onset of Action, Maintenance, and Recovery of Three Weight-based Dosing of Cisatracurium in Patients with Morbid Obesity in Laparoscopic Bariatric Surgery: A Randomized Clinical Trial. 在腹腔镜减重手术的病态肥胖患者中,比较三种基于体重的顺阿曲库铵剂量的起效、维持和恢复情况:随机临床试验
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2023.96131.2762
Faranak Rokhtabnak, Saeed Safari, Soudabeh Djalali Motlagh, Toktam Yavari, Elham Pardis

Background: For patients with morbid obesity, different cisatracurium dosage regimens are recommended. This study aimed to compare the onset of action, the sufficiency of neuromuscular blockade during infusion, and the recovery of the three distinct cisatracurium dosage scalars in patients with morbid obesity undergoing laparoscopic bariatric surgery.

Methods: In this randomized clinical trial, 55 patients were scheduled for bariatric surgery at Firoozgar Hospital from March 2020 to August 2021. Using a block randomization method, they were randomly divided into three groups, based on total body weight (TBW group), fat-free mass (FFM group), or ideal body weight (IBW group), to receive a bolus of cisatracurium 0.2 mg/Kg, followed by an infusion of 2 µg/Kg, to maintain a train-of-four (TOF) count≤2. Data were analyzed using SPSS software. P<0.05 was considered statistically significant.

Results: The mean time (seconds) to reach TOF0 in the TBW group was significantly shorter (201.89, 95%CI=192.99-210.79; P=0.004) than the IBW group (233.53, 95%CI=218.71-248.34; P=0.01). However, this difference was not statistically significant between TBW and FFM groups (220.83, 95%CI=199.73-241.94; P=0.81) or between FFM and the IBW groups (P=0.23). The rescue dose and increments of cisatracurium infusion were not required in the TBW group, whereas their probability was 4.81 times higher in the IBW group than the FFM group. Furthermore, the TBW and FFM groups had higher mean surgical condition scores than the IBW group (P<0.001, and P=0.006, respectively).

Conclusion: Cisatracurium loading and infusion dosing based on FFM provide a comparable onset of action and surgical field condition to the TBW-based dosing with a shorter recovery time. However, IBW-based dosing of cisatracurium was insufficient for laparoscopic bariatric surgery.Trial Registration Number: IRCT20151107024909N9.A preprint of this study was published at . doi: .

背景:对于病态肥胖患者,建议采用不同的顺阿曲库铵剂量方案。本研究旨在比较三种不同顺阿曲库铵剂量标度在接受腹腔镜减肥手术的病态肥胖患者中的起效时间、输注过程中神经肌肉阻滞的充分性以及恢复情况:在这项随机临床试验中,55 名患者计划于 2020 年 3 月至 2021 年 8 月在菲罗兹加医院接受减肥手术。根据总重量(TBW 组)、去脂体重(FFM 组)或理想体重(IBW 组),采用分块随机法将他们随机分为三组,分别接受顺阿曲库铵 0.2 毫克/千克的栓注,然后输注 2 微克/千克,以维持四列数(TOF)≤2。数据使用 SPSS 软件进行分析。结果TBW 组达到 TOF0 的平均时间(秒)(201.89,95%CI=192.99-210.79;P=0.004)明显短于 IBW 组(233.53,95%CI=218.71-248.34;P=0.01)。但是,TBW 组和 FFM 组之间(220.83,95%CI=199.73-241.94;P=0.81)或 FFM 组和 IBW 组之间(P=0.23)的差异无统计学意义。TBW 组无需使用顺阿曲库铵,而 IBW 组使用顺阿曲库铵的概率是 FFM 组的 4.81 倍。此外,TBW 组和 FFM 组的平均手术条件评分也高于 IBW 组(结论:TBW 组和 FFM 组的平均手术条件评分高于 IBW 组):基于 FFM 的顺阿曲库铵加载和输注剂量与基于 TBW 的剂量具有相似的起效时间和手术视野条件,且恢复时间更短。然而,基于 IBW 的顺阿曲库铵剂量不足以用于腹腔镜减肥手术。试验登记号:IRCT20151107024909N9.本研究的预印本发表于.doi: .
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引用次数: 0
Molecular Serotyping and Antibiotic Resistance Profile of Group B Streptococcus Strains Isolated from Iranian Pregnant Women with Urinary Tract Infection. 从患有尿路感染的伊朗孕妇中分离出的 B 组链球菌菌株的分子血清型和抗生素耐药性概况。
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/ijms.2023.96346.2787
Natalie Gharabeigi, Akram Sadat Tabatabaee Bafroee, Kumarss Amini

Background: Group B Streptococcus (GBS) can cause serious infections in neonates and pregnant women. GBS may cause urinary tract infections (UTIs). However, molecular epidemiology of such infections is rarely reported. The present study aimed to determine drug resistance patterns and molecular serotyping of GBS isolates in a population of pregnant Iranian women with UTIs.

Methods: A cross-sectional study was conducted during the first half of 2021 in the Department of Biology, East Tehran Branch, Islamic Azad University (Tehran, Iran). Sixty GBS strains isolated from the urine and placenta samples of pregnant women with UTIs were evaluated. The women were aged 19-46 years old at 35 to 37 weeks of gestation. The molecular serotype of GBS isolates was determined using a multiplex polymerase chain reaction, and the disc diffusion method was used to determine the antibiotic susceptibility pattern of isolates for different antibiotics. The association of the GBS serotype with the phenotype of antibiotic resistance was statistically analyzed using SPSS software (version 22.0) with a Chi square test and Cramer's V test. P<0.05 was considered statistically significant.

Results: GBS capsular serotype II was most prevalent (66.7%) followed by serotypes Ib (21.7%), Ia (3.3%), and III (1.7%). The prevalence of non-typeable isolates was significantly low (6.6%). Of the 60 GBS isolates, 18.3% were resistant to penicillin, 81.6% to ampicillin, 23.3% to clindamycin, and 30% to vancomycin; indicating the need for treatment alternatives.

Conclusion: Region-specific information on antibiotic resistance and molecular characteristics of GBS is essential for epidemiological investigations, effective treatment, and vaccine development.

背景:B 组链球菌 (GBS) 可导致新生儿和孕妇发生严重感染。GBS 可能会引起尿路感染(UTI)。然而,此类感染的分子流行病学却鲜有报道。本研究旨在确定伊朗孕妇尿路感染人群中 GBS 分离物的耐药模式和分子血清型:2021 年上半年,伊朗阿扎德伊斯兰大学东德黑兰分校生物系(伊朗德黑兰)开展了一项横断面研究。对从患有尿毒症的孕妇的尿液和胎盘样本中分离出的 60 株 GBS 进行了评估。这些孕妇的年龄在 19-46 岁之间,孕周在 35-37 周之间。使用多重聚合酶链反应确定了分离出的 GBS 分子血清型,并使用盘扩散法确定了分离出的菌株对不同抗生素的敏感性模式。使用 SPSS 软件(22.0 版)对 GBS 血清型与抗生素耐药表型的相关性进行了统计分析,并进行了 Chi Square 检验和 Cramer's V 检验。结果GBS 胶囊血清型 II 最常见(66.7%),其次是血清型 Ib(21.7%)、Ia(3.3%)和 III(1.7%)。不可分型分离物的流行率明显较低(6.6%)。在 60 个 GBS 分离物中,18.3% 对青霉素耐药,81.6% 对氨苄西林耐药,23.3% 对克林霉素耐药,30% 对万古霉素耐药;这表明需要替代治疗方法:结论:有关 GBS 抗生素耐药性和分子特征的特定地区信息对于流行病学调查、有效治疗和疫苗开发至关重要。
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引用次数: 0
The Added-on of Ziziphus jujube Syrup in the Treatment of Chronic Spontaneous Urticaria Resistant to Standard-Dose of Secondary-generation H1 Antihistamine: A Double-Blind Randomized Clinical Trial. 酸枣仁糖浆在治疗对标准剂量第二代 H1 抗组胺药耐药的慢性自发性荨麻疹中的附加作用:双盲随机临床试验。
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2023.95531.2690
Hamid Zare, Seyed Hesamedin Nabavizdeh, Amir Mohamad Jaladat, Mohamad Mehdi Zarshenas, Mozhgan Moghtaderi, Atefeh Basirat, Nahid Nasri

Background: Although antihistamines are the first-line treatment for chronic spontaneous urticaria (CSU), 50% of patients don't respond to standard doses. In this study, the effectiveness of Ziziphus jujube fruit syrup in combination with antihistamines was assessed in patients with CSU.

Methods: This double-blind randomized clinical trial was conducted in Shiraz between December 2019 and December 2020. 64 patients with CSU who had experienced hives for at least six weeks and did not respond to the usual treatments were enrolled in the study. They were randomly assigned to intervention and control groups using permuted block random allocation. For four weeks, the intervention group received 7.5 mL Ziziphus jujube syrup twice a day, while the control group received 7.5 mL simple jujube syrup twice a day. Both groups received cetirizine 10 mg every night. Urticaria activity score (UAS) and CU-Q2oL questionnaires were used to assess urticaria state and sleep quality before and after each week for four consecutive weeks. Data were analyzed using SPSS software version 18, and P<0.05 was considered statistically significant.

Results: Before the intervention, there was no statistically significant difference between the two groups' mean of UAS (P=0.490) and sleep quality (P=0.423). During the follow-up, UAS in the intervention group was significantly lower (P=0.001). Moreover, this difference was significant on the day 28 (P=0.046). During the follow-up, the quality of sleep in both groups improved significantly, and this improvement was more significant in the intervention group.

Conclusion: Ziziphus jujube syrup could be an effective adjuvant treatment for CSU.Trial Registration Number: IRCT20190304042916N1.

背景:尽管抗组胺药是慢性自发性荨麻疹(CSU)的一线治疗方法,但50%的患者对标准剂量无效。本研究评估了酸枣仁糖浆与抗组胺药联合使用对CSU患者的疗效:这项双盲随机临床试验于 2019 年 12 月至 2020 年 12 月在设拉子进行。64名患有荨麻疹至少6周且对常规治疗无效的CSU患者被纳入研究。他们被随机分配到干预组和对照组。在为期四周的时间里,干预组每天两次服用 7.5 毫升酸枣仁糖浆,对照组每天两次服用 7.5 毫升单味酸枣仁糖浆。两组每晚均服用西替利嗪 10 毫克。连续四周,每周前后使用荨麻疹活动评分(UAS)和CU-Q2oL问卷评估荨麻疹状态和睡眠质量。数据使用 SPSS 软件 18 版进行分析:干预前,两组 UAS 平均值(P=0.490)和睡眠质量(P=0.423)差异无统计学意义。在随访期间,干预组的 UAS 显著降低(P=0.001)。此外,这一差异在第 28 天也很明显(P=0.046)。随访期间,两组的睡眠质量均有明显改善,干预组的改善更为显著:试验注册号:IRCT20190304042916N1。
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引用次数: 0
Pregnancy in the Unicornuate Uterus and Non-Communicating Rudimentary Horn. 单角子宫和不沟通的直角子宫妊娠。
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2023.97859.2971
Salmeh Dadgar, Maliheh Mahmoudinia, Neda Davaryari
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引用次数: 0
A Novel Arg120Pro Mutation in the RP2 Gene in an Iranian Family with X-linked Retinitis Pigmentosa: A Case Report. 一个伊朗 X 连锁视网膜色素变性家族中 RP2 基因的新型 Arg120Pro 突变:病例报告。
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2022.96392.2792
Nasrin Mansouri, Parichehr Darabi, Masoumeh Favaedi, Hanieh Faizmahdavi, Soheila Nankali, Marjan Assefi, Alireza Sharafshah, Vahid Omarmeli

As the most common type of inherited retinal degenerative disease, retinitis pigmentosa (RP) has taken clinical and prenatal attention. Considering the clinical importance of consanguineous marriages, new mutations in this type of pregnancy have a high risk and increase the importance of Prenatal Diagnosis (PND). In vitro analysis was done through Whole Exome Sequencing (WES) for a 36-year-old woman who was referred to a genetic laboratory in Kermanshah in 2021 for PND. The woman had consanguineous marriage and was pregnant with twins (a boy and a girl). Mutation confirmation tests were also performed on her husband and both fetuses to find mutations. Moreover, in silico analyses were performed by SWISS-MODEL, ProSA, Molprobity, Swiss-Pdb Viewer, and ERRAT. The WES analysis showed a novel mutation of the RP2 gene (exon2:c. 359G>C: p.R120P) in the 36-year-old pregnant woman. Mutations identified in her husband and her twins revealed changes in protein conformations. Further modeling and validation evaluations showed the replacement of Arg by Pro at the 120th residue site of the cognate protein. For the first time, our report introduced a novel missense mutation in the RP2 gene associated with severe signs of RP in an Iranian family based on an X-linked recessive pattern of genetic inheritance. These findings may pave the way for a better diagnosis of RP in genetic counseling and PND.

视网膜色素变性(RP)是最常见的遗传性视网膜变性疾病,已引起临床和产前关注。考虑到近亲结婚在临床上的重要性,这种类型的妊娠发生新突变的风险很高,也增加了产前诊断(PND)的重要性。2021 年,一名 36 岁的妇女因 PND 转诊到克尔曼沙赫的一家基因实验室,通过全外显子组测序(WES)对其进行了体外分析。该妇女为近亲结婚,并怀有一对双胞胎(一男一女)。为了找到突变,还对她的丈夫和两个胎儿进行了突变确认检测。此外,还利用 SWISS-MODEL、ProSA、Molprobity、Swiss-Pdb Viewer 和 ERRAT 进行了硅学分析。WES 分析显示,这名 36 岁孕妇的 RP2 基因有一个新的突变(外显子 2:c. 359G>C:p.R120P)。在其丈夫和双胞胎体内发现的突变显示了蛋白质构象的变化。进一步的建模和验证评估显示,在同源蛋白的第 120 个残基位点,Arg 被 Pro 取代。我们的报告首次介绍了伊朗一个家族中与严重 RP 症状相关的 RP2 基因错义突变,该突变具有 X 连锁隐性遗传模式。这些发现可能为在遗传咨询和 PND 中更好地诊断 RP 铺平了道路。
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引用次数: 0
The Role of Circular RNAs in Male Infertility and Reproductive Cancers: A Narrative Review. 环状 RNA 在男性不育症和生殖系统癌症中的作用:叙述性综述。
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2022.95302.2661
Zahra Derakhshan, Soghra Bahmanpour, Sanaz Alaee, Jafar Fallahi, Seyed Mohammad Bagher Tabei

Infertility is a global health problem affecting about 15% of all couples, of which 50% are due to male infertility. Although the etiology of infertility is known in most infertile men, idiopathic male infertility remains a challenge. Therefore, there is a need for novel diagnostic methods to detect the underlying mechanisms and develop appropriate therapies. Recent studies have focused on the role of non-coding RNAs (ncRNAs) in male infertility. Circular RNAs (CircRNAs), a type of ncRNAs, are found to play a key role in the development of some pathological conditions, including cardiovascular diseases, diabetes, cancers, autoimmune diseases, etc. Several studies have reported the presence of CircRNAs and their target genes in the human reproductive system. In addition, their expression in testicular tissues, sperm cells, and seminal fluid has been identified. Abnormal expression of CircRNAs has been associated with azoospermia and asthenozoospermia in infertile men. The present narrative review provides a brief description of the role of CircRNAs in spermatogenic cells, male infertility, and reproductive cancers. In addition, some CircRNAs have been identified as potential biomarkers for disease detection and treatment.

不孕不育是一个全球性的健康问题,影响着约 15%的夫妇,其中 50%是由于男性不育造成的。虽然大多数不育男性的不育病因已明,但特发性男性不育仍是一个难题。因此,需要新的诊断方法来检测其潜在机制并开发适当的疗法。最近的研究集中于非编码 RNA(ncRNA)在男性不育中的作用。环状 RNA(CircRNA)是 ncRNA 的一种,在心血管疾病、糖尿病、癌症、自身免疫性疾病等病症的发生发展中起着关键作用。一些研究报告称,CircRNAs 及其靶基因存在于人类生殖系统中。此外,它们在睾丸组织、精子细胞和精液中的表达也已被确定。CircRNAs 的异常表达与不育男性的无精子症和无精子症有关。本综述简要介绍了 CircRNAs 在生精细胞、男性不育症和生殖系统癌症中的作用。此外,一些 CircRNAs 已被确定为检测和治疗疾病的潜在生物标志物。
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引用次数: 0
Genotoxic and Cytotoxic Effects of Cone Beam Computed Tomography and Multidetector Computed Tomography on Exfoliated Buccal Epithelial Cells. 锥形束计算机断层扫描和多载体计算机断层扫描对脱落颊上皮细胞的基因毒性和细胞毒性效应
IF 1.6 Q2 MEDICINE, GENERAL & INTERNAL Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2023.96522.2807
Zahra Jahanshahiafshar, Hakimeh Ghorbani, Maryam Seyedmajidi, Mehrdad Nabahati, Kourosh Ebrahimnejad Gorji, Seyedali Seyedmajidi, Ehsan Moudi

Background: Cone beam computed tomography (CBCT) and multidetector computed tomography (MDCT) are frequently used in dental and maxillofacial problems. This study aimed to assess the genotoxicity and cytotoxicity effects of CBCT and MDCT radiographies on exfoliated buccal epithelial cells during dental examinations.

Methods: This prospective experimental study was conducted at Babol University of Medical Sciences (Babol, Iran) from March 2021 to April 2021. Buccal mucosa smears were collected bilaterally pre-exposure and 12 days after CBCT or MDCT examinations. To compare the frequency of micronuclei and other cytotoxic cellular changes such as pyknosis, karyolysis, and karyorrhexis, the paired sample t test and Wilcoxon test were used. In addition, independent sample t test, Mann-Whitney, and Chi square tests were used to investigate the differences between the imaging methods and between men and women. All statistical analyses were performed using the SPSS software, and P≤0.05 was considered statistically significant.

Results: The current study included 60 adult patients (30 patients in each group), ranging in age from 21 to 50 years. The micronuclei and the other cytotoxic cellular changes increased significantly after CBCT and MDCT radiographic examinations on the 12th day compared to the pre-exposure results (P<0.001). MDCT had statistically higher cytotoxic and genotoxic effects than CBCT (9.4%, 23.1%, and 40% higher values in micronucleus frequency, the mean frequency of micronuclei, and other cytotoxic changes, respectively). There were no significant differences between men and women in the two examination methods (P=0.46 and P=0.49, respectively).

Conclusion: Dental examinations with CBCT and MDCT can increase cytotoxicity and chromosomal damage in both men and women. Due to its lower radiation toxicities, CBCT can be recommended as an alternative to MDCT for dental examinations.

背景:锥形束计算机断层扫描(CBCT)和多载体计算机断层扫描(MDCT)经常用于牙科和颌面部问题。本研究旨在评估 CBCT 和 MDCT 放射摄影对牙科检查过程中脱落的口腔上皮细胞的遗传毒性和细胞毒性影响:这项前瞻性实验研究于 2021 年 3 月至 2021 年 4 月在巴博勒医科大学(伊朗巴博勒)进行。在接触前和 CBCT 或 MDCT 检查后 12 天收集双侧颊粘膜涂片。为了比较微核和其他细胞毒性细胞变化(如细胞凋亡、核溶解和核分裂)的频率,采用了配对样本 t 检验和 Wilcoxon 检验。此外,还使用独立样本 t 检验、曼-惠特尼检验和卡方检验来研究不同成像方法之间以及男女之间的差异。所有统计分析均使用 SPSS 软件进行,P≤0.05 为差异有统计学意义:本次研究包括 60 名成年患者(每组 30 人),年龄从 21 岁到 50 岁不等。与暴露前的结果相比,第 12 天进行 CBCT 和 MDCT 放射检查后,微核和其他细胞毒性变化明显增加(PC 结论:CBCT 和 MDCT 放射检查的结果与暴露前的结果相比,微核和其他细胞毒性变化明显增加:使用 CBCT 和 MDCT 进行牙科检查会增加男性和女性的细胞毒性和染色体损伤。由于 CBCT 的辐射毒性较低,建议在牙科检查中使用 CBCT 替代 MDCT。
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引用次数: 0
Iranian Journal of Medical Sciences at a Glance: The Past and Present. 伊朗医学科学杂志概览》:过去与现在。
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/ijms.2023.49644
Mohammad Reza Panjehshahin
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引用次数: 0
Gastrointestinal Manifestations of Lead Poisoning: A Brief Report. 铅中毒的胃肠道表现:简要报告。
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2023.95824.2749
Masumeh Safaee, Mahdi Malekzadeh, Narges Motamedi, Masoud Sayadishahraki, Nastaran Eizadi-Mood

Gastrointestinal (GI) manifestations of lead poisoning include abdominal pain, constipation, and diarrhea. Depending on the severity of a symptom, surgical consultation is required. The present study aimed to make a comparison between the mean blood lead levels of patients hospitalized for lead toxicity and the various Gl symptoms. A retrospective cross-sectional study was performed in 2020 at Khorshid Hospital, the main regional referral center for poisoned patients (Isfahan, Iran). A total of 82 patients aged ≥18 years who were hospitalized for lead poisoning during 2017-2018 were included in the study. Patients' information was extracted from hospital medical records, including demographic information, clinical manifestations, blood lead levels, and treatment outcome. The mean age of the patients was 48.18±11.9 years, 91.5% were men, and 62.2% suffered from multiple GI symptoms, with abdominal pain being predominant (31.7%). Blood lead levels in patients with multiple GI symptoms were higher than those with only one symptom (P=0.01). Surgical consultation was required in 14.6% of the patients. Multiple GI symptoms were the main predictive factor for blood lead levels above 70 mg/dL (P=0.03, Odds ratio=3.06, 95% CI=1.09-8.61). Given the prevalence of abdominal pain and its association with elevated blood lead levels, differential diagnosis of abdominal pain should include lead toxicity.

铅中毒的胃肠道(GI)表现包括腹痛、便秘和腹泻。根据症状的严重程度,需要进行外科会诊。本研究旨在比较因铅中毒住院患者的平均血铅水平和各种 Gl 症状。这项回顾性横断面研究于 2020 年在中毒患者的主要地区转诊中心 Khorshid 医院(伊朗伊斯法罕)进行。研究共纳入了 2017-2018 年期间因铅中毒住院的 82 名年龄≥18 岁的患者。患者信息均从医院病历中提取,包括人口统计学信息、临床表现、血铅水平和治疗结果。患者的平均年龄为(48.18±11.9)岁,91.5%为男性,62.2%的患者有多种消化道症状,其中以腹痛为主(31.7%)。有多种消化道症状的患者血铅含量高于只有一种症状的患者(P=0.01)。14.6%的患者需要进行手术治疗。多种消化道症状是血铅水平超过 70 mg/dL 的主要预测因素(P=0.03,Odds ratio=3.06,95% CI=1.09-8.61)。鉴于腹痛的普遍性及其与血铅水平升高的关系,腹痛的鉴别诊断应包括铅中毒。
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引用次数: 0
Potential Role of Zinc Finger 365 rs10822013 and rs10995190 in Mammographic Density, Sporadic Breast Cancer Risk, and Prognosis. 锌指365 rs10822013 和 rs10995190 在乳腺密度、散发性乳腺癌风险和预后中的潜在作用。
IF 2.4 Q1 Pharmacology, Toxicology and Pharmaceutics Pub Date : 2023-11-01 DOI: 10.30476/IJMS.2023.96141.2767
Seyed Reza Ghadamgahi, Leila Hosseinzadeh, Sahar Ardalan Khales, Mohammadreza Nassiri, Maryam Alidoust, Shirin Etemadrezaei, Asma Khorshid Shamshiri, Fatemeh Homaei Shandiz, Alireza Pasdar, Fahimeh Afzaljavan

Background: Despite suggesting many genetic risk markers as the outcome of Genome-wide association studies (GWAS) for breast cancer, replicating the results in different populations has remained the main issue. In this regard, this study assessed the association of two variations in Zinc Finger 365 (ZNF365) in an Iranian population.

Methods: In a case-control study conducted at Mashhad University of Medical Sciences, Mashhad, Iran, between 2017 and 2020, ZNF365-rs10822013 and rs10995190 were genotyped using Allele-Specific PCR (AS-PCR). Breast density was assessed using mammography images. PHASE software module version 2 and SPSS version 16.0 were used for haplotype and statistical analyses. Quantitative and qualitative variables were compared between groups using independent t tests and Chi square tests, respectively. Binary logistic regression analysis was performed to calculate odds ratios. Multivariate analysis was then undertaken for the baseline variables, with a P<0.05 in the univariate analysis. The survival analysis was performed using the Kaplan-Meier method and the log-rank test.

Results: In this survey, 732 females, including 342 breast cancer patients and 390 healthy subjects, were enrolled. rs10822013-T allele (P=0.014), rs10995190-G allele (P=0.003), and TG haplotype (P=0.002) were significantly associated with the increased risk of breast cancer. Moreover, rs10995190-GG genotype (P=0.042) and C-G haplotype (P=0.019) revealed a significant association with better overall survival. However, considered polymorphisms and their haplotypes indicated no association with breast density and clinical features of breast cancer.

Conclusion: ZNF365 variants might be a potential risk marker of breast cancer in the Iranian population. The interaction between alleles in haplotypes may modulate the amount of the risk conferred by these variants. Further studies on different ethnic groups can validate these results.

背景:尽管乳腺癌全基因组关联研究(GWAS)提出了许多遗传风险标记,但在不同人群中复制研究结果仍是一个主要问题。为此,本研究评估了伊朗人群中锌指 365(ZNF365)的两个变异的关联性:在伊朗马什哈德市马什哈德医科大学于 2017 年至 2020 年进行的一项病例对照研究中,使用等位基因特异性 PCR(AS-PCR)对 ZNF365-rs10822013 和 rs10995190 进行了基因分型。乳房密度使用乳房 X 射线摄影图像进行评估。使用 PHASE 软件模块 2 版和 SPSS 16.0 版进行单倍型和统计分析。组间定量和定性变量的比较分别采用独立 t 检验和卡方检验。二元逻辑回归分析用于计算几率比。然后对基线变量进行多变量分析,得出结果:rs10822013-T等位基因(P=0.014)、rs10995190-G等位基因(P=0.003)和TG单倍型(P=0.002)与乳腺癌风险增加显著相关。此外,rs10995190-GG 基因型(P=0.042)和 C-G 单倍型(P=0.019)与较好的总生存率也有明显关系。然而,考虑的多态性及其单倍型与乳腺密度和乳腺癌的临床特征没有关联:ZNF365变异可能是伊朗人患乳腺癌的潜在风险标志物。单倍型中等位基因之间的相互作用可能会调节这些变异所带来的风险。对不同种族群体的进一步研究可以验证这些结果。
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引用次数: 0
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Iranian Journal of Medical Sciences
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