IGF-I, Insulin like growth factor-I is a multi-functional polypeptide which organizes the proliferation and differentiation of various cell types and has a metabolic activity similar to insulin. We know that IGF-I receptors localize in the gastrointestinal tract. Polyamines play an important role in the gastrointestinal growth which connect with IGF-I. DFMO, I±-difluoromethylornithine is an irreversible inhibitor of ODC, ornithine decarboxylase, the rate-limiting enzyme in the synthesis of polyamines. In the IGF-I-promoted growth the role of polyamines mainly investigate in vitro systems, however, in vivo studies are necessary to clarify the subject. Our aim in this study is to examine the in vivo role of IGF-I as a stimulant in the growth of developing rat's small intestine, and to clarify the role of polyamines in the period of growth promoted by IGF-I. In this study, animals were divided into 2 groups. DFMO (500 mg/kg/day) was administered for 10 days to 9 rats forming the experimental group. Sterile 0.9% NaCl was injected at the same volume as for the experimental animals into 8 rats forming the control group. Tissue samples were taken from the rats for histological, immunohistochemical and biochemical analyses on the 10th day. In the group given DFMO, the expansion and compression of the villi, as well as a decrease in the PAS-positive reaction intensity of brush border and in the number of goblet cells were observed. mmunohistochemical assessments showed that the number of PCNA, ODC and IGF-I positive cells in the DFMO-treated group decreased significantly, compared with the control group. Biochemical investigations showed that DNA values in the small intestinal tissue decreased insignificantly in the DFMO treated group as compared to the control one. We concluded that application of DFMO in the developing rat small intestine inhibited polyamine synthesis and IGF-I synthesis and partially prevented growth of the intestine. Keywords: Polyamine, I±-difluoromethylornithine, insulin-like growth factor-I, ornithine decarboxylase
{"title":"The role of insulin-like growth factor-I and polyamines in developing rat small intestine","authors":"O. Karabulut-Bulan, Ş. Bolkent","doi":"10.18478/IUFSJB.99580","DOIUrl":"https://doi.org/10.18478/IUFSJB.99580","url":null,"abstract":"IGF-I, Insulin like growth factor-I is a multi-functional polypeptide which organizes the proliferation and differentiation of various cell types and has a metabolic activity similar to insulin. We know that IGF-I receptors localize in the gastrointestinal tract. Polyamines play an important role in the gastrointestinal growth which connect with IGF-I. DFMO, I±-difluoromethylornithine is an irreversible inhibitor of ODC, ornithine decarboxylase, the rate-limiting enzyme in the synthesis of polyamines. In the IGF-I-promoted growth the role of polyamines mainly investigate in vitro systems, however, in vivo studies are necessary to clarify the subject. Our aim in this study is to examine the in vivo role of IGF-I as a stimulant in the growth of developing rat's small intestine, and to clarify the role of polyamines in the period of growth promoted by IGF-I. In this study, animals were divided into 2 groups. DFMO (500 mg/kg/day) was administered for 10 days to 9 rats forming the experimental group. Sterile 0.9% NaCl was injected at the same volume as for the experimental animals into 8 rats forming the control group. Tissue samples were taken from the rats for histological, immunohistochemical and biochemical analyses on the 10th day. In the group given DFMO, the expansion and compression of the villi, as well as a decrease in the PAS-positive reaction intensity of brush border and in the number of goblet cells were observed. mmunohistochemical assessments showed that the number of PCNA, ODC and IGF-I positive cells in the DFMO-treated group decreased significantly, compared with the control group. Biochemical investigations showed that DNA values in the small intestinal tissue decreased insignificantly in the DFMO treated group as compared to the control one. We concluded that application of DFMO in the developing rat small intestine inhibited polyamine synthesis and IGF-I synthesis and partially prevented growth of the intestine. \u0000Keywords: Polyamine, I±-difluoromethylornithine, insulin-like growth factor-I, ornithine decarboxylase","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83864963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the morpho-anatomical features of Colchicum boissieri Orph. were demonstrated. The morphological characteristics of the corm, leaf, flower, fruit and seeds were presented and the habitus of the species were given by photographs. Corm lengths, cataphyll color and length, leaf color, outer-inner tepal dimension (length x width), perigonium tube dimension, outer-inner filament (length) and anther (length), styles color, seed number, shape, dimension and color were determined. The corm of C. boissieri is soboliferous, occasionally tooth-like projections, vertical, soboles horizontal, 2.3-4.5 cm in length and 0.6-1.4 cm in width. The neck (collum) of the corm is 3-5.5 cm in length. The cataphyll is yellowish-white in color, 3-7.5 cm in length. Perigonium tube is 4.4-13 cm in length. The seeds are that wrinkled surface subglobose to globose in shape, yellowish-brown in color, and (1.3-) 1.4-1.9 mm in diameter. Anatomical characteristics of the corm, leaf, fruit and seeds were shown with photographs. Transverse sections from the middle portion of the corm, leaf, pericarp and seed testa were obtained. The upper and lower surface views of the leaf, pericarp and the seed testa were examined. Anatomical features of C. boissieri were discussed in this research for the first time. Anatomical features of C. boissieri are similar to some Colchicum. In the anatomical view of leaf, it has been determined that there are differences in the number of spongy parenchyma cell layers between the species. Testa of the seeds include epidermis, parenchyma and pigment layer cells. Key words: Colchicum, morphology, anatomy, soboliferous
{"title":"Morpho-anatomical observations on Colchicum boissieri Orph. in","authors":"E. Sevgi, Orhan Küçüker","doi":"10.18478/IUFSJB.35892","DOIUrl":"https://doi.org/10.18478/IUFSJB.35892","url":null,"abstract":"In this study, the morpho-anatomical features of Colchicum boissieri Orph. were demonstrated. The morphological characteristics of the corm, leaf, flower, fruit and seeds were presented and the habitus of the species were given by photographs. Corm lengths, cataphyll color and length, leaf color, outer-inner tepal dimension (length x width), perigonium tube dimension, outer-inner filament (length) and anther (length), styles color, seed number, shape, dimension and color were determined. The corm of C. boissieri is soboliferous, occasionally tooth-like projections, vertical, soboles horizontal, 2.3-4.5 cm in length and 0.6-1.4 cm in width. The neck (collum) of the corm is 3-5.5 cm in length. The cataphyll is yellowish-white in color, 3-7.5 cm in length. Perigonium tube is 4.4-13 cm in length. The seeds are that wrinkled surface subglobose to globose in shape, yellowish-brown in color, and (1.3-) 1.4-1.9 mm in diameter. \u0000Anatomical characteristics of the corm, leaf, fruit and seeds were shown with photographs. Transverse sections from the middle portion of the corm, leaf, pericarp and seed testa were obtained. The upper and lower surface views of the leaf, pericarp and the seed testa were examined. Anatomical features of C. boissieri were discussed in this research for the first time. Anatomical features of C. boissieri are similar to some Colchicum. In the anatomical view of leaf, it has been determined that there are differences in the number of spongy parenchyma cell layers between the species. Testa of the seeds include epidermis, parenchyma and pigment layer cells. \u0000Key words: Colchicum, morphology, anatomy, soboliferous","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86188405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The parameters of the length-weight relationship of the form W = aL b are presented for 8 species of crustaceans caught along the Sigacik Bay, Aegean Sea coast of Turkey. Samples from commercial trawlers were taken during September 2005 to July 2006 . The ‘b' value ranged between 0.568 and 3.156.
本文给出了土耳其爱琴海沿岸Sigacik湾捕获的8种甲壳类动物的长度-重量关系式W = aL - b的参数。在2005年9月至2006年7月期间从商业拖网渔船采集样本。b值在0.568 ~ 3.156之间。
{"title":"Length-weight relationship of eight decapod crustaceans of the Sığacık Bay, Aegean Sea coast of Turkey","authors":"Tahir Özcan, T. Katağan","doi":"10.18478/IUFSJB.17950","DOIUrl":"https://doi.org/10.18478/IUFSJB.17950","url":null,"abstract":"The parameters of the length-weight relationship of the form W = aL b are presented for 8 species of crustaceans caught along the Sigacik Bay, Aegean Sea coast of Turkey. Samples from commercial trawlers were taken during September 2005 to July 2006 . The ‘b' value ranged between 0.568 and 3.156.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74094380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Said, A. Huefner, El-Sayed Ali Abu tabl, G. Fawzy
In this study, three phenolic compounds were isolated from the seeds of Zizyphus spina-christi for the first time. The compounds were identified by means of 1 H-NMR-, UV and GC-MS as p -hydroxybenzoic acid, kaempferol , quercetin-3-O- a -L-rhamnosyl- b -D-glucopyranoside (rutin) .
本研究首次从茜草种子中分离得到3个酚类化合物。通过1h - nmr、UV和GC-MS鉴定化合物为对羟基苯甲酸、山奈酚、槲皮素-3- o - a - l-鼠李糖基- b - d -葡萄糖吡喃苷(芦丁)。
{"title":"Phenolic compounds from seeds of Zizyphus spina-christi","authors":"A. Said, A. Huefner, El-Sayed Ali Abu tabl, G. Fawzy","doi":"10.18478/IUFSJB.00166","DOIUrl":"https://doi.org/10.18478/IUFSJB.00166","url":null,"abstract":"In this study, three phenolic compounds were isolated from the seeds of Zizyphus spina-christi for the first time. The compounds were identified by means of 1 H-NMR-, UV and GC-MS as p -hydroxybenzoic acid, kaempferol , quercetin-3-O- a -L-rhamnosyl- b -D-glucopyranoside (rutin) .","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85513426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Glucagon-like peptide-2 (GLP-2) is a peptide hormone with intestinotrophic activity, which is released from the intestinal endocrine cells. The aim of this study was to investigate the effects observed by scanning electron microscope of GLP-2 in intestinal epithelial injury induced by tumor necrosis factor-alpha (TNF-α)/actinomycin D (Act D). In addition, it aimed to elucidate whether the action mechanism of GLP-2 might be mediated by gastrointestinal hormones such as cholecystokinin and somatostatin . The intestinal epithelial injury was induced by intraperitoneal administration of 15 µg/kg TNF-α and 800 µg/kg Act D per mouse. Animals were injected subcutaneously 200 µg/kg GLP-2 analogue every 12 hr for 10 consecutive days prior to the administration of TNF-α and Act D. Scanning electron microscopic examination revealed severe epithelial damage in the small intestine of TNF-α/Act D-administered mice. The administration of TNF-α/Act D was significantly increased the number of somatostatin -immunoreactive endocrine cells, but did not affect the number of cholecystokinin -immunoreactive endocrine cells in the intestinal mucosa . On the other hand, GLP-2 analogue pretreatment prevented TNF-α/Act D-induced intestinal epithelial injury by causing a marked decrease in the degenerative changes and the number of somatostatin -immunoreactive endocrine cells, and a significant increase in the number of cholecystokinin -immunoreactive endocrine cells. As a result, the present study indicates that GLP-2 has a protective effect against TNF-α/Act D-induced intestinal epithelial injury. Morever protective effect of GLP-2 might be related to somatostatin and cholecystokinin.
{"title":"Effects of glucagon-like peptide-2 on TNF-alpha/actinomycin D-induced intestinal epithelial injury: a scanning electron microscopic and immunohistochemical study","authors":"P. A. Pirincci, P. Turan, S. Arbak, Ş. Bolkent","doi":"10.18478/IUFSJB.91609","DOIUrl":"https://doi.org/10.18478/IUFSJB.91609","url":null,"abstract":"Glucagon-like peptide-2 (GLP-2) is a peptide hormone with intestinotrophic activity, which is released from the intestinal endocrine cells. The aim of this study was to investigate the effects observed by scanning electron microscope of GLP-2 in intestinal epithelial injury induced by tumor necrosis factor-alpha (TNF-α)/actinomycin D (Act D). In addition, it aimed to elucidate whether the action mechanism of GLP-2 might be mediated by gastrointestinal hormones such as cholecystokinin and somatostatin . The intestinal epithelial injury was induced by intraperitoneal administration of 15 µg/kg TNF-α and 800 µg/kg Act D per mouse. Animals were injected subcutaneously 200 µg/kg GLP-2 analogue every 12 hr for 10 consecutive days prior to the administration of TNF-α and Act D. Scanning electron microscopic examination revealed severe epithelial damage in the small intestine of TNF-α/Act D-administered mice. The administration of TNF-α/Act D was significantly increased the number of somatostatin -immunoreactive endocrine cells, but did not affect the number of cholecystokinin -immunoreactive endocrine cells in the intestinal mucosa . On the other hand, GLP-2 analogue pretreatment prevented TNF-α/Act D-induced intestinal epithelial injury by causing a marked decrease in the degenerative changes and the number of somatostatin -immunoreactive endocrine cells, and a significant increase in the number of cholecystokinin -immunoreactive endocrine cells. As a result, the present study indicates that GLP-2 has a protective effect against TNF-α/Act D-induced intestinal epithelial injury. Morever protective effect of GLP-2 might be related to somatostatin and cholecystokinin.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79987246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ibrahim, I. Alaraidh, A. Amid, A. Farouk, S. Bazaid, R. Greiner, Abdullah Alghunaim
A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia using PCR and agarose gel visualization. The samples were tested for the existence of the NOS Terminator. A total of 150 samples were tested out of which three samples tested positive as GM-rice which were retested in Malaysia. The presence of GMO rice in Saudi Arabia supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops.
{"title":"Detection of genetically modified rice: Collaborative validation study of a PCR based detection of genetically modified rice Oryza sativa commercially available in Saudi Arabia","authors":"M. Ibrahim, I. Alaraidh, A. Amid, A. Farouk, S. Bazaid, R. Greiner, Abdullah Alghunaim","doi":"10.18478/IUFSJB.73474","DOIUrl":"https://doi.org/10.18478/IUFSJB.73474","url":null,"abstract":"A collaborative trial study has been conducted for validation of an extraction method and a subsequent PCR for the detection of transgenic rice sold in Saudi Arabia. The tests were carried out in Saudi Arabia using Real-Time PCR and the positive samples were validated in another lab in Malaysia using PCR and agarose gel visualization. The samples were tested for the existence of the NOS Terminator. A total of 150 samples were tested out of which three samples tested positive as GM-rice which were retested in Malaysia. The presence of GMO rice in Saudi Arabia supports the necessity of developing precise quantitative and qualitative ways for routine analyses and detection of GMO products in the Saudi Arabian market. With the discovery of GM products in the Saudi Arabian market it would be of no surprise that other Middle Eastern nations also knowingly or unknowingly import GM crops.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84173853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study is to examine the effects of the neurosecretory material in corpus cardiacum (CC) on the reproduction of endoparasitic Pimpla turionellae L. For this purpose, the egg maturation and amount of the neurosecretory material in corpus cardiacum of the insects were examined by the serial cross sections. Egg maturation was determined by measuring the terminal oocyte length in the serial cross sections of the ovariole. The egg growth which has observed on the 3 rd day, reached the maximum on 15 th day and after that day the egg laying was observed. The sections of insect brains were stained by paraldehyde fuchsin . The amount of the paraldehyde fuchsin positive neurosecretory (PF + NS) material in CC was at a certain level in the first day of egg development. While terminal oocyte was reaching the maximum length, the amount of the PF + NS material in CC decreased to the minimum level. During the egg laying phase, the amount of the PF + NS material in CC reached the maximum level. These observations give us the idea that the neurosecretory material in corpus cardiacum of this insect may be related to the egg development.
{"title":"The paraldehyde fuchsin positive material in corpora cardiaca of Pimpla turionellae L. (Hymenoptera:Ichneumonidae) during egg maturation","authors":"Aydin Ozluk, N. Gül","doi":"10.18478/IUFSJB.49924","DOIUrl":"https://doi.org/10.18478/IUFSJB.49924","url":null,"abstract":"The purpose of this study is to examine the effects of the neurosecretory material in corpus cardiacum (CC) on the reproduction of endoparasitic Pimpla turionellae L. For this purpose, the egg maturation and amount of the neurosecretory material in corpus cardiacum of the insects were examined by the serial cross sections. Egg maturation was determined by measuring the terminal oocyte length in the serial cross sections of the ovariole. The egg growth which has observed on the 3 rd day, reached the maximum on 15 th day and after that day the egg laying was observed. The sections of insect brains were stained by paraldehyde fuchsin . The amount of the paraldehyde fuchsin positive neurosecretory (PF + NS) material in CC was at a certain level in the first day of egg development. While terminal oocyte was reaching the maximum length, the amount of the PF + NS material in CC decreased to the minimum level. During the egg laying phase, the amount of the PF + NS material in CC reached the maximum level. These observations give us the idea that the neurosecretory material in corpus cardiacum of this insect may be related to the egg development.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84064403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Belivermiş, Ö. Kılıç, T. Engizek, S. Ergenç, A. Çayır
In the current work, Cd, Co, Cr, Cu, Fe, Ni, Pb and Zn concentrations were measured in the feed samples of the dairy cattle by means of atomic absorption spectrophotometer. For this purpose a total of 47 common vetch, hay, concentrate feed and wheat samples were collected from the villages of Thrace region. The average concentrations of Cd, Co, Cr, Cu, Fe, Ni, Pb and Zn were found as 1.15 ± 0.19, 3.22 ± 0.64, 4.51 ± 1.60, 3.46 ± 0.94, 90.6 ± 13.5, 7.1 ± 0.4, 9.2 ± 3.9, 42.2 ± 9.2 mg kg -1 , respectively, in dry matter. The highest concentrations of Cu, Fe, Zn were found in the concentrate feed samples while the highest concentrations of Cd, Co, Cr, Ni, Pb have been found in the hay samples. Factor analysis was performed in order to distinguish sources of measured elements. High positive correlation coefficients were observed among Cd, Co, Cr, Ni, Pb amounts of the feed. Average Cd concentrations of common vetch, hay and wheat samples were found higher than limit value recommended by dairy food organization .
本文采用原子吸收分光光度计测定了奶牛饲料样品中Cd、Co、Cr、Cu、Fe、Ni、Pb和Zn的浓度。为此目的，从色雷斯地区的村庄共收集了47个普通紫薇、干草、浓缩饲料和小麦样品。干物质中Cd、Co、Cr、Cu、Fe、Ni、Pb、Zn的平均浓度分别为(1.15±0.19)、(3.22±0.64)、(4.51±1.60)、(3.46±0.94)、(90.6±13.5)、(7.1±0.4)、(9.2±3.9)、(42.2±9.2)mg kg -1。精料样品中Cu、Fe、Zn含量最高，干草样品中Cd、Co、Cr、Ni、Pb含量最高。为了区分所测元素的来源，进行了因子分析。饲料中Cd、Co、Cr、Ni、Pb含量呈高度正相关。普通野豌豆、干草和小麦样品的平均镉浓度高于乳制品组织推荐的限量值。
{"title":"Trace element concentrations in animal feed samples from Thrace region, Turkey","authors":"M. Belivermiş, Ö. Kılıç, T. Engizek, S. Ergenç, A. Çayır","doi":"10.18478/IUFSJB.41340","DOIUrl":"https://doi.org/10.18478/IUFSJB.41340","url":null,"abstract":"In the current work, Cd, Co, Cr, Cu, Fe, Ni, Pb and Zn concentrations were measured in the feed samples of the dairy cattle by means of atomic absorption spectrophotometer. For this purpose a total of 47 common vetch, hay, concentrate feed and wheat samples were collected from the villages of Thrace region. The average concentrations of Cd, Co, Cr, Cu, Fe, Ni, Pb and Zn were found as 1.15 ± 0.19, 3.22 ± 0.64, 4.51 ± 1.60, 3.46 ± 0.94, 90.6 ± 13.5, 7.1 ± 0.4, 9.2 ± 3.9, 42.2 ± 9.2 mg kg -1 , respectively, in dry matter. The highest concentrations of Cu, Fe, Zn were found in the concentrate feed samples while the highest concentrations of Cd, Co, Cr, Ni, Pb have been found in the hay samples. Factor analysis was performed in order to distinguish sources of measured elements. High positive correlation coefficients were observed among Cd, Co, Cr, Ni, Pb amounts of the feed. Average Cd concentrations of common vetch, hay and wheat samples were found higher than limit value recommended by dairy food organization .","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89167990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The loss of islet cells by apoptotic cell death plays a central role in the pathogenesis of experimental animal models such as hyperglycemia and diabetes. Streptozotocin (STZ) is widely used for the induction of diabetes in animals by destruction of pancreatic i¢ cells. The aim of the present study is to determine the effects of different dose and administration time of STZ on pancreatic islet cells. With this aim, four different experimental groups consist of the animals treated with citrate buffer (0.01 M, pH: 4.5) only, the animals sacrificed after 5 hours of the 40 mg/kg STZ injection, the animals sacrificed after 6 hours of the 60 mg/kg STZ injection, and the animals sacrificed after 4 hours of the 75mg/kg STZ injection were composed. While the apoptotic cells were observed by TUNEL staining method and according to morphologic criteria of the cells, insulin synthesized cells were shown by immunohistochemical technique. As the result it was determined an increase in blood glucose levels and apoptotic islet cells in 40 and 75 mg/kg STZ-injected rats while islet and beta cell areas were decreased in all groups. These results indicate that 40 and 75 mg/kg STZ injection at the end of 5 and 4 hours, respectively, causes hyperglycemia by triggering apoptosis in islet cells of adult rats. Keywords: Apoptosis, hyperglycemia, islet cell, rat, streptozotocin.
{"title":"The apoptotic effects of Streptozotocin in different dose and administration time on pancreatic islet cells of rats","authors":"S. Gezginci-Oktayoglu","doi":"10.18478/IUFSJB.23700","DOIUrl":"https://doi.org/10.18478/IUFSJB.23700","url":null,"abstract":"The loss of islet cells by apoptotic cell death plays a central role in the pathogenesis of experimental animal models such as hyperglycemia and diabetes. Streptozotocin (STZ) is widely used for the induction of diabetes in animals by destruction of pancreatic i¢ cells. The aim of the present study is to determine the effects of different dose and administration time of STZ on pancreatic islet cells. With this aim, four different experimental groups consist of the animals treated with citrate buffer (0.01 M, pH: 4.5) only, the animals sacrificed after 5 hours of the 40 mg/kg STZ injection, the animals sacrificed after 6 hours of the 60 mg/kg STZ injection, and the animals sacrificed after 4 hours of the 75mg/kg STZ injection were composed. While the apoptotic cells were observed by TUNEL staining method and according to morphologic criteria of the cells, insulin synthesized cells were shown by immunohistochemical technique. As the result it was determined an increase in blood glucose levels and apoptotic islet cells in 40 and 75 mg/kg STZ-injected rats while islet and beta cell areas were decreased in all groups. These results indicate that 40 and 75 mg/kg STZ injection at the end of 5 and 4 hours, respectively, causes hyperglycemia by triggering apoptosis in islet cells of adult rats. Keywords: Apoptosis, hyperglycemia, islet cell, rat, streptozotocin.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78628486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Histology offers a powerful tool in the study of detailed structures of different tissues in fishes. It is used for sex verification, identifying stages of development, presence of parasites, tumors diagnosis, other abnormalities and changes in the arrangement of tissue layers including the digestive tract. In this study, not having found any existing report, the digestive tract histology of the Persian tooth-carp, Aphanius persicus (Jenkins, 1910), was investigated. The dentition comprised canine-like teeth in one row with sharp tips. Esophagus consisted of small portion between pharynx and stomach. Stomach showed an enlargement and the intestine is relatively short. The mucosa of total digestive tube consisted of simple columnar cells and numerous goblet cells. The liver is composed of parenchymal cells and lattice fibers whose function is to support the former. Our data showed this fish is heterodont, omnivores with no secretory stomach.
{"title":"Histological and Morphological Studies of digestive tube and liver of the Persian tooth-carp, Aphanius persicus(Actinopterygii: Cyprinodontidae)","authors":"M. Monsefi, Z. Gholami, H. Esmaeili","doi":"10.18478/IUFSJB.06589","DOIUrl":"https://doi.org/10.18478/IUFSJB.06589","url":null,"abstract":"Abstract Histology offers a powerful tool in the study of detailed structures of different tissues in fishes. It is used for sex verification, identifying stages of development, presence of parasites, tumors diagnosis, other abnormalities and changes in the arrangement of tissue layers including the digestive tract. In this study, not having found any existing report, the digestive tract histology of the Persian tooth-carp, Aphanius persicus (Jenkins, 1910), was investigated. The dentition comprised canine-like teeth in one row with sharp tips. Esophagus consisted of small portion between pharynx and stomach. Stomach showed an enlargement and the intestine is relatively short. The mucosa of total digestive tube consisted of simple columnar cells and numerous goblet cells. The liver is composed of parenchymal cells and lattice fibers whose function is to support the former. Our data showed this fish is heterodont, omnivores with no secretory stomach.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86856326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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