Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol bas
漆酶的研究主要集中在担子菌科的木腐真菌中，特别是在白腐真菌中。对其他真菌科的研究在很大程度上缺乏。本研究在以儿茶酚为基础的培养基中评价了里氏木霉漆酶的活性。结果表明，青苔漆酶在酸性pH范围内具有活性，最适pH为4.5。在含10 gL -1儿茶酚的培养基中，漆酶的活性为1.22 U ml -1，比含10 gL -1葡萄糖的培养基高6倍以上。同时测定了不同儿茶酚浓度下的漆酶活性。在15 gL -1浓度下，漆酶活性略有下降，最大活性为1.1 Uml -1。结果表明，在以儿茶酚为碳源的培养基中，灰杉漆酶活性高于葡萄糖。漆酶的研究主要集中在担子菌科的木腐真菌中，特别是在白腐真菌中。对其他真菌科的研究在很大程度上缺乏。本研究在以儿茶酚为基础的培养基中评价了里氏木霉漆酶的活性。结果表明，青苔漆酶在酸性pH范围内具有活性，最适pH为4.5。在含10 gL -1儿茶酚的培养基中，漆酶的活性为1.22 U ml -1，比含10 gL -1葡萄糖的培养基高6倍以上。同时测定了不同儿茶酚浓度下的漆酶活性。在15 gL -1浓度下，漆酶活性略有下降，最大活性为1.1 Uml -1。结果表明，在以儿茶酚为碳源的培养基中，灰杉漆酶活性高于葡萄糖。漆酶的研究主要集中在担子菌科的木腐真菌中，特别是在白腐真菌中。对其他真菌科的研究在很大程度上缺乏。本研究在以儿茶酚为基础的培养基中评价了里氏木霉漆酶的活性。结果表明，青苔漆酶在酸性pH范围内具有活性，最适pH为4.5。在含10 gL -1儿茶酚的培养基中，漆酶的活性为1.22 U ml -1，比含10 gL -1葡萄糖的培养基高6倍以上。同时测定了不同儿茶酚浓度下的漆酶活性。在15 gL -1浓度下，漆酶活性略有下降，最大活性为1.1 Uml -1。结果表明，在以儿茶酚为碳源的培养基中，灰杉漆酶活性高于葡萄糖。漆酶的研究主要集中在担子菌科的木腐真菌中，特别是在白腐真菌中。对其他真菌科的研究在很大程度上缺乏。本研究在以儿茶酚为基础的培养基中评价了里氏木霉漆酶的活性。结果表明，青苔漆酶在酸性pH范围内具有活性，最适pH为4.5。在含10 gL -1儿茶酚的培养基中，漆酶的活性为1.22 U ml -1，比含10 gL -1葡萄糖的培养基高6倍以上。同时测定了不同儿茶酚浓度下的漆酶活性。在15 gL -1浓度下，漆酶活性略有下降，最大活性为1.1 Uml -1。结果表明，在以儿茶酚为碳源的培养基中，灰杉漆酶活性高于葡萄糖。
{"title":"Studies on Laccase Activity in the Filamentous Fungus Trichoderma reesei","authors":"Semra Sekme, N. Ataci, Inci Arisan","doi":"10.18478/IUFSJB.50042","DOIUrl":"https://doi.org/10.18478/IUFSJB.50042","url":null,"abstract":"Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol based medium. Results showed that laccase enzyme from T.reesei was active in acidic pH range and that optimum pH was 4.5. The optimum temperature of laccase from T. reesei was also 27 0 C. Laccase activity in medium containing 10 gL -1 catechol was 1.22 U ml -1 , which was more than 6 times higher than in medium containing 10 gL -1 glucose. Laccase activity of T.reesei was also determined in different catechol concentrations. At a concentration of 15 gL -1 , laccase activity slightly decreased and the obtained maximum activity was 1.1 Uml -1 . Laccase activity of T. reesei was found higher than glucose, in the medium containing catechol as carbon source. Laccase have been mainly studied in wood rot fungal species of the basidiomycetes family especially in white rot fungi. Studies in other fungal families are largely lacking. This study has evaluated laccase activity from Trichoderma reesei in catechol bas","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88609627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Etrusk sivrifaresi, Suncus etruscus ozellikle bati ve guney Anadolu bolgelerinde kaydedilmistir. Guneydogu Anadolu bolgesinin farkli lokalitelerinden toplanan, cesitli baykus Asio otus, Athene noctua, Bubo bubo ve Tyto alba’ya ait peletler incelenmistir. Kalintilar icerisinde % 1.03 oraninda Suncus etruscus turune rastlanmistir. Ayrica Diyarbakir yakininda Karacadag’dan bir canli ornek elde edilmistir. Bu calismanin bulgulari Guneydogu Anadolu bolgesi icin yeni kayittir.
{"title":"Additional records of Suncus etruscus (Savi, 1822) (Mammalia: Soricidae) from Southeastern Anatolia, Turkey","authors":"Y. Coşkun, A. Kaya","doi":"10.18478/IUFSJB.70979","DOIUrl":"https://doi.org/10.18478/IUFSJB.70979","url":null,"abstract":"Etrusk sivrifaresi, Suncus etruscus ozellikle bati ve guney Anadolu bolgelerinde kaydedilmistir. Guneydogu Anadolu bolgesinin farkli lokalitelerinden toplanan, cesitli baykus Asio otus, Athene noctua, Bubo bubo ve Tyto alba’ya ait peletler incelenmistir. Kalintilar icerisinde % 1.03 oraninda Suncus etruscus turune rastlanmistir. Ayrica Diyarbakir yakininda Karacadag’dan bir canli ornek elde edilmistir. Bu calismanin bulgulari Guneydogu Anadolu bolgesi icin yeni kayittir.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87655471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laccase ( EC 1.10.3.2) is a multi copper enzyme that catalyzes the oxidation of various environmental pollutants such as phenolic compounds. The efficiency of the enzyme for environmental and industrial applications can be increased by immobilizing the enzyme on a carrier. In the present study, laccase obtained from Trametes versicolor (fungi) was immobilized on sodium alginate beads and kappacarrageenan and the effect of contact time, pH, temperature, the amount of carrier and enzyme concentration were investigated to determine optimum conditions of laccase immobilization. Sodium alginate beads were chosen as the most efficient carrier due to their high immobilization yield. Maximum laccase immobilization was determined as 30 min of contact time, pH 4.5, 30 °C of temperature, 200 mg of sodium alginate amount.
{"title":"Determination of Optimum Immobilization Conditions of Trametes versicolor Laccase with Sodium Alginate Beads","authors":"Arzu Ünal, N. Kolankaya","doi":"10.18478/IUFSJB.12484","DOIUrl":"https://doi.org/10.18478/IUFSJB.12484","url":null,"abstract":"Laccase ( EC 1.10.3.2) is a multi copper enzyme that catalyzes the oxidation of various environmental pollutants such as phenolic compounds. The efficiency of the enzyme for environmental and industrial applications can be increased by immobilizing the enzyme on a carrier. In the present study, laccase obtained from Trametes versicolor (fungi) was immobilized on sodium alginate beads and kappacarrageenan and the effect of contact time, pH, temperature, the amount of carrier and enzyme concentration were investigated to determine optimum conditions of laccase immobilization. Sodium alginate beads were chosen as the most efficient carrier due to their high immobilization yield. Maximum laccase immobilization was determined as 30 min of contact time, pH 4.5, 30 °C of temperature, 200 mg of sodium alginate amount.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90672576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Balkıs, N. Sivri, Linda Fraim, M. Balcı, Turgay Durmuş, A. Sukatar
In the summer of 2010, the excessive growth of Cladophora laetevirens along the southwestern shoreline of Istanbul, which carries great importance for tourism, drew major attention. The aim of this study was to investigate the excessive growth of Cladophora laetevirens , some ecological parameters, and the number of total coliform bacteria of surface waters in this region during the summer of 2010. During this period, this species multiplied and mucilage like environment was formed and the total coliform bacteria values were found to be between 10 5 and 10 8 MPN/100 mL. Due to the lack of available evidence prior to the sampling, it is possible that the excessive mucilage formation may have been trigged by Cladophora laetevirens . Cladophora samples were collected on different sampling dates during the 2010 summer (July and August). On these sampling days, the temperature, salinity and dissolved oxygen levels of the seawater ranged between 26.6-28°C, 14-33 ppt, and 8.2-9.7 mg/L, respectively. Nitrate+nitrite-N (0.21- 0.26 μg-at/L), Ammonium-N (0.43-0.94 μg-at/L), Phosphate-P (0.53-3.50 μg-at/L), Silicate-Si (9.63- 15.30 μg-at/L) and Chlorophyll a (3.68-14.11 μg/L) concentrations were measured.
{"title":"Excessive growth of Cladophora laetevirens (Dillwyn) Kutzing and enteric bacteria in mats in the Southwestern Istanbul coast, Sea of Marmara","authors":"N. Balkıs, N. Sivri, Linda Fraim, M. Balcı, Turgay Durmuş, A. Sukatar","doi":"10.18478/IUFSJB.44760","DOIUrl":"https://doi.org/10.18478/IUFSJB.44760","url":null,"abstract":"In the summer of 2010, the excessive growth of Cladophora laetevirens along the southwestern shoreline of Istanbul, which carries great importance for tourism, drew major attention. The aim of this study was to investigate the excessive growth of Cladophora laetevirens , some ecological parameters, and the number of total coliform bacteria of surface waters in this region during the summer of 2010. During this period, this species multiplied and mucilage like environment was formed and the total coliform bacteria values were found to be between 10 5 and 10 8 MPN/100 mL. Due to the lack of available evidence prior to the sampling, it is possible that the excessive mucilage formation may have been trigged by Cladophora laetevirens . Cladophora samples were collected on different sampling dates during the 2010 summer (July and August). On these sampling days, the temperature, salinity and dissolved oxygen levels of the seawater ranged between 26.6-28°C, 14-33 ppt, and 8.2-9.7 mg/L, respectively. Nitrate+nitrite-N (0.21- 0.26 μg-at/L), Ammonium-N (0.43-0.94 μg-at/L), Phosphate-P (0.53-3.50 μg-at/L), Silicate-Si (9.63- 15.30 μg-at/L) and Chlorophyll a (3.68-14.11 μg/L) concentrations were measured.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74942008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The insect fat body is the major biosynthetic and storage organ involved in lipid, carbohydrate, amino acid and nitrogen metabolism and protein synthesis. It also actively participates in vitellogenesis and ovary development, synthesizing the soluble precursor for yolk, i.e., vitellogenin in stage specific manner. The continual substance exchange among fat body, hemolymph and ovary is controlled by hormones, ecdysteroids and juvenile hormone, during metamorphosis of Bombyx mori (L.). The aim of the present study was to clarify the interactions between major proteins of fat body and hemolymph and their effect on ovarian development under hormonal factors during pupal-adult transformation. Detected fat body, hemolymph and ovary proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE): storage proteins (72 kDa and 76 kDa), 30 kDa proteins, ApoLp-I (230-250 kDa), egg specific protein (72 kDa and 64 kDa) and vitellogenins (178 kDa). Our results suggest that changes in the well-defined and characterized protein fractions quality and quantity of the fat body had a direct effect under hormonal factors on the ovary and egg development during metamorphosis.
{"title":"Investigation of the Interaction between Fat body and Ovary Development during Pupal Transformation in Silkworm, Bombyx mori","authors":"Esen Poyraz, E. Goncu, O. Parlak","doi":"10.18478/IUFSJB.04765","DOIUrl":"https://doi.org/10.18478/IUFSJB.04765","url":null,"abstract":"The insect fat body is the major biosynthetic and storage organ involved in lipid, carbohydrate, amino acid and nitrogen metabolism and protein synthesis. It also actively participates in vitellogenesis and ovary development, synthesizing the soluble precursor for yolk, i.e., vitellogenin in stage specific manner. The continual substance exchange among fat body, hemolymph and ovary is controlled by hormones, ecdysteroids and juvenile hormone, during metamorphosis of Bombyx mori (L.). The aim of the present study was to clarify the interactions between major proteins of fat body and hemolymph and their effect on ovarian development under hormonal factors during pupal-adult transformation. Detected fat body, hemolymph and ovary proteins are grouped as follows by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE): storage proteins (72 kDa and 76 kDa), 30 kDa proteins, ApoLp-I (230-250 kDa), egg specific protein (72 kDa and 64 kDa) and vitellogenins (178 kDa). Our results suggest that changes in the well-defined and characterized protein fractions quality and quantity of the fat body had a direct effect under hormonal factors on the ovary and egg development during metamorphosis.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91035461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Okuyan, F. Izzettin, Özlem Bingöl-Ozakpinar, P. Turan, Z. Ozdemir, M. Sancar, Z. Cirakli, P. Clark, F. Ercan
The study was aimed at investigating the possible renoprotective effects of Ginkgo biloba on nephrotoxicity induced by cisplatin w/wo other antineoplastic agents (etoposide and gemcitabine) in rats. The animals were randomly divided into eight groups each consisting of six rats. Serum blood urea nitrogen (BUN) and creatinine values at baseline and after drug administration, kidney malondialdehyde (MDA), glutathione (GSH) levels, and myeloperoxidase activity (MPO) were measured, and a histopathologic examination of kidney tissues was carried out. Ginkgo biloba extract significantly decreased the serum creatinine and kidney MDA levels, which had increased as a result of cisplatin administration and also improved the depletion of kidney GSH levels in cisplatin administered rats (p<0.05). These results were confirmed by histopathologic observations of the kidney tissues. According to the results of the present study, the potential interactions between the renoprotective agents and the cisplatin-based chemotherapeutic regimens must be considered. Keywords: Cisplatin- induced nephrotoxicity, Etoposide, Gemcitabine, Ginkgo biloba,
{"title":"The effects of Ginkgo biloba on nephrotoxicity induced by cisplatin-based chemotherapy protocols in rats","authors":"B. Okuyan, F. Izzettin, Özlem Bingöl-Ozakpinar, P. Turan, Z. Ozdemir, M. Sancar, Z. Cirakli, P. Clark, F. Ercan","doi":"10.18478/IUFSJB.02974","DOIUrl":"https://doi.org/10.18478/IUFSJB.02974","url":null,"abstract":"The study was aimed at investigating the possible renoprotective effects of Ginkgo biloba on nephrotoxicity \u0000induced by cisplatin w/wo other antineoplastic agents (etoposide and gemcitabine) in rats. The \u0000animals were randomly divided into eight groups each consisting of six rats. Serum blood urea nitrogen \u0000(BUN) and creatinine values at baseline and after drug administration, kidney malondialdehyde (MDA), \u0000glutathione (GSH) levels, and myeloperoxidase activity (MPO) were measured, and a histopathologic \u0000examination of kidney tissues was carried out. Ginkgo biloba extract significantly decreased the serum \u0000creatinine and kidney MDA levels, which had increased as a result of cisplatin administration and also \u0000improved the depletion of kidney GSH levels in cisplatin administered rats (p<0.05). These results were \u0000confirmed by histopathologic observations of the kidney tissues. According to the results of the present \u0000study, the potential interactions between the renoprotective agents and the cisplatin-based chemotherapeutic \u0000regimens must be considered. \u0000Keywords: Cisplatin- induced nephrotoxicity, Etoposide, Gemcitabine, Ginkgo biloba,","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73087363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Dehghani, Aseyeh Heshmatpour, M. Panjehshahin, T. Talaei-Khozani
Syzygium aromaticum was considered as an aphrodisiac agent; however, it may cause some side effects. The aim of this study was to examine the effects of Syzygium aromaticum extract on male reproductive system. To do this, forty male mice were divided into 5 groups: negative control was fed with vehicle, positive control was fed with aphridite and three experimental groups were fed with 250, 500 and 1000 mg/kg/day of water/alcohol extract of S. aromaticum for 34 days. The sperm count and motility and also the sex hormones level were assessed. The reproductive tissues were prepared histologically and studied under the light microscope. The results indicated the high dose-treated animals showed a significant decline in sperm count, motility and testosterone but a significant increase in estradiol concentration compared with the control group. The seminiferous tubules of extract-treated animals contained fewer sperms than in those of control animals. It seems that in spite of aphrodiastic activity of the Syzygium aromaticum extract, it reduced spermatogenesis. Keywords: Reproduction, Syzygium aromaticum, Sperm
{"title":"Toxic effects of water/alcoholic extract of Syzygium aromaticum on sperm quality, sex hormones and reproductive tissues in male mouse","authors":"F. Dehghani, Aseyeh Heshmatpour, M. Panjehshahin, T. Talaei-Khozani","doi":"10.18478/IUFSJB.82989","DOIUrl":"https://doi.org/10.18478/IUFSJB.82989","url":null,"abstract":"Syzygium aromaticum was considered as an aphrodisiac agent; however, it may cause some side effects. \u0000The aim of this study was to examine the effects of Syzygium aromaticum extract on male reproductive \u0000system. To do this, forty male mice were divided into 5 groups: negative control was fed with vehicle, \u0000positive control was fed with aphridite and three experimental groups were fed with 250, 500 and 1000 \u0000mg/kg/day of water/alcohol extract of S. aromaticum for 34 days. The sperm count and motility and also \u0000the sex hormones level were assessed. The reproductive tissues were prepared histologically and studied \u0000under the light microscope. The results indicated the high dose-treated animals showed a significant \u0000decline in sperm count, motility and testosterone but a significant increase in estradiol concentration \u0000compared with the control group. The seminiferous tubules of extract-treated animals contained fewer \u0000sperms than in those of control animals. It seems that in spite of aphrodiastic activity of the Syzygium \u0000aromaticum extract, it reduced spermatogenesis. \u0000Keywords: Reproduction, Syzygium aromaticum, Sperm","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82984561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Centaurea L. (Asteraceae) is one of the largest genera in the flora of Turkey. Centaurea arifolia belonging to the genus Centaurea is considered as DD (Data Deficient) according to IUCN category. There are no studies identified aiming to propagate this species in vitro. In this study, the seeds were germinated aseptically in Petri dishes containing growth regulator free Murashige and Skoog half-strength (MS ½) medium. Leaf explants of six-week-old grown seedlings were cultured on MS medium supplemented with 1 mg l−1 BAP plus 0.1 mg l−1 NAA and 2 mg l−1 BAP plus 0.2 mg l−1 NAA for indirect regeneration and after three weeks, actively growing calli appeared and proliferated. Induction of adventitious shoot regeneration from calli was achieved approximately 150% (MS1) and 120% (MS2), within three weeks. Regenerated adventitious shoots were best rooted on MS medium containing 1 mg l−1 IBA. This protocol provides a successful propagation technique through indirect in vitro organogenesis from leaf segments of C. arifolia. Keywords: C. arifolia, endemic species, micropropagation
{"title":"In vitro propagation of endemic plant Centaurea arifolia Boiss. Taxa","authors":"E. Yüzbaşıoğlu, E. Dalyan, M. Bona, G. Öz","doi":"10.18478/IUFSJB.73052","DOIUrl":"https://doi.org/10.18478/IUFSJB.73052","url":null,"abstract":"Centaurea L. (Asteraceae) is one of the largest genera in the flora of Turkey. Centaurea arifolia belonging \u0000to the genus Centaurea is considered as DD (Data Deficient) according to IUCN category. There are \u0000no studies identified aiming to propagate this species in vitro. In this study, the seeds were germinated \u0000aseptically in Petri dishes containing growth regulator free Murashige and Skoog half-strength (MS ½) \u0000medium. Leaf explants of six-week-old grown seedlings were cultured on MS medium supplemented \u0000with 1 mg l−1 BAP plus 0.1 mg l−1 NAA and 2 mg l−1 BAP plus 0.2 mg l−1 NAA for indirect regeneration \u0000and after three weeks, actively growing calli appeared and proliferated. Induction of adventitious \u0000shoot regeneration from calli was achieved approximately 150% (MS1) and 120% (MS2), within three \u0000weeks. Regenerated adventitious shoots were best rooted on MS medium containing 1 mg l−1 IBA. This \u0000protocol provides a successful propagation technique through indirect in vitro organogenesis from leaf \u0000segments of C. arifolia. \u0000Keywords: C. arifolia, endemic species, micropropagation","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78031325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study aimed to investigate antioxidant and cyclooxygenase (COX) inhibitory activities of Ailanthus altissima leaves extracts. N-hexane and methanol-aqueous extracts of Ailanthus altissima leaves were tested for their antioxidant activities, using DPPH (1,1-Diphenyl-2-picrylhydrazyl), ferric reducing antioxidant power, cholinesterase, elastase, and tyrosinase assays. The results showed that methanolaqueous extract has a significant radical scavenging activity, reducing power, and mild cyclooxygenase (COX) inhibitory activity and n-hexane was less active. Keywords: Ailanthus altissima, leaves, antioxidant activity, cyclooxygenase (COX) inhibitory activity.
{"title":"Antioxidant and cyclooxygenase (COX) inhibitory activities of Ailanthus altissima (Mill.) Swingle leaves","authors":"Y. Seo, Bong-Ho Lee, K. Rashed, A. Said","doi":"10.18478/IUFSJB.44499","DOIUrl":"https://doi.org/10.18478/IUFSJB.44499","url":null,"abstract":"The present study aimed to investigate antioxidant and cyclooxygenase (COX) inhibitory activities of Ailanthus altissima leaves extracts. N-hexane and methanol-aqueous extracts of Ailanthus altissima leaves were tested for their antioxidant activities, using DPPH (1,1-Diphenyl-2-picrylhydrazyl), ferric reducing antioxidant power, cholinesterase, elastase, and tyrosinase assays. The results showed that methanolaqueous extract has a significant radical scavenging activity, reducing power, and mild cyclooxygenase (COX) inhibitory activity and n-hexane was less active. Keywords: Ailanthus altissima, leaves, antioxidant activity, cyclooxygenase (COX) inhibitory activity.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73147491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, biofilm formation on different type of epoxidized soybean oil (ESO) based polymers was investigated. Different types of ESO based polymers were synthesized. Bacterial accumulations on surface of these polymers were determined by using Modified Robbins Device for 60 days period. Surface characterizations of the materials were also performed at the beginning, at the 30th day and the 60th day of the experiment. Except ESO-HMDA polymer, changes were observed on the other polymer surfaces. Among the polymers synthesized, bacterial accumulation was the highest on the surfaces of the ESO-PEGM and ESO-TETA polymers. On the other hand, biofilm formation on the ESO-BPA and AESO-acrylic acid copolymer was the lowest. After 60 days, however, bacterial accumulation was highest on the surfaces of the ESO-TETA, ESO-MA and ESO-BPA polymers. Changes of the functional groups on the surface of the polymers were followed by MIR spectroscopy. It was found that almost all the surfaces of the materials were changed by microorganisms. When the surface was invaded by bacteria, microorganisms began to consume triglyceride moiety first.
{"title":"Microbial biofilm formation on soybean oil based polymers","authors":"Gökhan Çaylı, Irfan Türetgen","doi":"10.18478/IUFSJB.83501","DOIUrl":"https://doi.org/10.18478/IUFSJB.83501","url":null,"abstract":"In this study, biofilm formation on different type of epoxidized soybean oil (ESO) based polymers was investigated. Different types of ESO based polymers were synthesized. Bacterial accumulations on surface of these polymers were determined by using Modified Robbins Device for 60 days period. Surface characterizations of the materials were also performed at the beginning, at the 30th day and the 60th day of the experiment. Except ESO-HMDA polymer, changes were observed on the other polymer surfaces. Among the polymers synthesized, bacterial accumulation was the highest on the surfaces of the ESO-PEGM and ESO-TETA polymers. On the other hand, biofilm formation on the ESO-BPA and AESO-acrylic acid copolymer was the lowest. After 60 days, however, bacterial accumulation was highest on the surfaces of the ESO-TETA, ESO-MA and ESO-BPA polymers. Changes of the functional groups on the surface of the polymers were followed by MIR spectroscopy. It was found that almost all the surfaces of the materials were changed by microorganisms. When the surface was invaded by bacteria, microorganisms began to consume triglyceride moiety first.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73921397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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