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Study of biofilm associated bacteria on polyvinyl chloride, stainless steel and glass surfaces in a model cooling tower system with different microbiological methods 用不同微生物学方法研究模型冷却塔系统中聚氯乙烯、不锈钢和玻璃表面生物膜相关细菌
Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.93915
Nazmiye Ozlem Sanli Yurudu
Cooling towers are an integral part of large range of industrial processes. The conditions of cooling water systems provide an ideal environment for microbial growth and biofilm formation. Microorganisms which enter via makeup water or air to the system form a biofilm layer on the inside surfaces of cooling tower which contain water and limited nutrients. Biofilm formation in cooling tower systems is undesirable for operational and public health reasons. Thus, microbial load should be monitored and kept under control. In this study, bulk water and biofilm associated microbial load were analyzed in terms of heterotrophic plate count (HPC), epifluorescence microscopy, carbohydrate quantity, total and free ATP concentration and compared material dependence. Kruskal-Wallis statistical analysis revealed that there were not significant differences between the tested slide materials according to HPC, DAPI-CTC staining, and ATP measurement. The ATP measurement together with fluorescence staining rei¬‚ected the changes in the bioi¬lms more distinctly than the HPC.
冷却塔是大范围工业过程的一个组成部分。冷却水系统的条件为微生物生长和生物膜的形成提供了理想的环境。微生物通过补给水或空气进入系统,在冷却塔内表面形成生物膜层,其中含有水和有限的营养物质。从运行和公共卫生的角度考虑,冷却塔系统中生物膜的形成是不可取的。因此,应监测并控制微生物负荷。在本研究中,从异养平板计数(HPC)、荧光显微镜、碳水化合物数量、总ATP和游离ATP浓度等方面分析了水和生物膜相关的微生物负荷,并比较了物质依赖性。Kruskal-Wallis统计分析显示,HPC染色、DAPI-CTC染色和ATP测量结果显示,被测载玻片材料之间无显著差异。ATP的测定和荧光染色比HPC更明显地反映了生物膜的变化。
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引用次数: 5
Antioxidant and antibacterial properties of a lichen species Diploschistes scruposus (Schreb.) Norman 一种地衣的抗氧化和抗菌特性诺曼
Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.47020
B. Sökmen, Sinem Aydın, K. Kınalıoğlu
Bu calismada, Diploschistes scruposus (Schreb.) Norman (Graphidaceae) likeninin antibakteriyal ve antioksidan ozellikleri arastirilmistir. D. scruposus likeninin etanol ekstresinin antioksidan aktivitesi bes farkli metodla calisilmistir: DPPH (2,2-difenil-1-pikrilhidrazil) radikal supurme aktivitesi, ABTS.+ (2,2'-azino-bis (3etilbenzotiyazolin-6-sulfonik asit) radikali supurme aktivitesi, indirgeme gucu ve toplam fenolik iceriginin belirlenmesi. Test ekstresinin degisik antioksidan aktiviteleri bilinen antioksidanlarla kiyaslanmistir. Liken ekstresinin %50 inhibisyon konsantrasyon degerleri (IC50), DPPH radikali supurme aktivitesi icin 54.37±0.71; ABTS.+ radikali supurme aktivitesi icin 45.45±0.80 olarak gozlenmistir. D. scruposus etanol ekstresinin 1 mg’inda 161.4±0.01 µg/mL gallik asit esdegeri toplam fenolik bilesikleri bulunmaktadir. Liken ekstresinin antibakteriyal aktivitesi agar kuyu difuzyon yontemine gore belirlenmistir. Maksimum antibakteriyal aktivite Bacillus subtilis’e, en dusuk antibakteriyal aktivite ise Escherichia coli’ye karsi gozlenmistir. Bu calisma, D. scruposus likeninin dogal antioksidan ve antibakteriyal kaynagi olarak kullanilabilecegini gostermistir
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引用次数: 8
Ecdysone receptor B1 in Bombyx mori L. (Lepidoptera:Bombycidae) prothoracic gland under various organ culture conditions 不同器官培养条件下家蚕前胸腺蜕皮激素受体B1的研究
Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.06808
Emre Batır, E. Goncu, O. Parlak
During the early last larval instar, hormone secretory activity of prothoracic glands in Bombyx mori L. is undetectable despite the high prothoracicotropic hormone (PTTH) level in hemolymph. Early reports indicated that the inhibition of gland activity is caused by 20-hydroxyecdysteroid (20E) peak on day 2 of the fourth larval instar and juvenile hormone (JH) only resumes this inactive period during the early days of 5th larval instar. Following these reports, prothoracic glands also become a target of its hormone, 20E. Actions of 20E are mediated via heterodimeric ecdysone receptor complex which consists of ecdysone receptor (EcR) and its partner molecule, ultraspiracle (USP). This study was designed to determine the expression pattern of ecdysone receptor B1 (EcR-B1) in the prothoracic glands of B. mori during last larval instar and early pupal stage. Organ culture experiments were carried out to clarify the effects of juvenile hormone and 20-hydroxyecdysone on the EcR-B1 expression. Immunocytochemical studies revealed the presence of EcR-B1 in prothoracic gland cells. A rise of EcR-B1 immoreactivity was detected on day 3 of 5th instar in which glands recover the secretion activity. Juvenile hormone administration to culture medium maintained EcR-B1 immunoreactivity especially on day 6 and day 9 when compared with control but in contrast to this result, an ecdysone application to culture medium weakened the immunoreactivity. These results suggested that negative feedback effect of ecdysone on secretory activity of the glands may be exerted by means of ecdysone receptor and it may also involve consistent and regular 20E secretion during the fifth instar.
家蚕晚期早期血淋巴中促胸激素(pth)水平较高,但前胸腺激素分泌活性不明显。早期研究表明,20-羟基外皮甾体(20E)在第4龄第2天达到峰值,而幼体激素(JH)在第5龄早期才恢复这一非活性期。根据这些报道,前胸腺也成为其激素20E的目标。20E的作用是通过异二聚体蜕皮激素受体复合物介导的,该复合物由蜕皮激素受体(EcR)及其伴侣分子超胞(USP)组成。本研究旨在测定家蚕前胸腺中蜕皮激素受体B1 (EcR-B1)在幼虫末龄和蛹期早期的表达规律。通过器官培养实验研究幼激素和20-羟基蜕皮激素对EcR-B1表达的影响。免疫细胞化学研究显示前胸腺细胞中存在EcR-B1。5龄第3天,腺体恢复分泌活性,EcR-B1免疫活性升高。与对照组相比,在培养基中添加幼鱼激素维持了EcR-B1的免疫反应性,特别是在第6天和第9天,但与此结果相反,在培养基中添加蜕皮激素削弱了免疫反应性。这些结果提示蜕皮激素对腺体分泌活性的负反馈作用可能是通过蜕皮激素受体发挥的,也可能与第5期持续规律的20E分泌有关。
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引用次数: 1
Antitumor activity of Ailanthus excelsa (Roxb) stem bark fractions and Canthin-6-one 臭椿茎皮部位和cantin -6- 1的抗肿瘤活性
Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.60321
A. Said, K. Rashed, H. Tokuda, A. Huefner
The alkaloidal compound was isolated from chloroform fraction of methanol 70% extract of Ailanthus excelsa stem bark of Egyptian origin. Successive extracts of A. excelsa stem bark and the isolated compound canthin-6-one were screened for chemopreventive activity. The chloroform extract showed strong inhibitory effect on short term in vivo assay for antitumor promotors, Epstein-Barr virus early antigen induction assay, compared with other fractions petroleum ether, diethyl ether and methanol 70% extracts. The isolated compound canthin-6-one also showed strong activity in the course of this assay. Further, these useful materials were investigated for the inhibitory effects in two-stage mouse skin carcinogensis test. Chloroform extract and its active canthin-6-one decrease actually the average number of papillomas per mouse and percentage papillomas in the promoting stage. These materials were found to exhibit the excellent anti-tumor promoting activity in the in vivo carcinogensis test.
该生物碱化合物是从埃及产臭椿茎皮70%甲醇提取液的氯仿部分分离得到的。对黄连茎皮连续提取液和分离得到的化合物canthin-6- 1进行化学预防活性筛选。与石油醚、乙醚和甲醇70%提取物相比,氯仿提取物在体内短期抗肿瘤启动子试验和eb病毒早期抗原诱导试验中表现出较强的抑制作用。分离得到的化合物canthin-6- 1在实验过程中也表现出较强的活性。进一步研究了这些有用材料在小鼠两阶段皮肤癌变试验中的抑制作用。氯仿提取物及其活性成分canin -6- 1在促进期平均每只小鼠乳头瘤数和百分比均有降低作用。在体内致癌试验中发现这些材料表现出良好的抗肿瘤促进活性。
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引用次数: 2
The effect of aminoguanidine on the kidney of diabetic albino Balb/c mice 氨基胍对糖尿病白化Balb/c小鼠肾脏的影响
Pub Date : 2012-10-05 DOI: 10.18478/IUFSJB.89070
Ebru Gurel, N. Yilmazer, Cihan Demirci-Tansel
The aim of this study is to find out how activated inducible nitric oxide synthase (iNOS) and nitric oxide (NO) affect kidney tissue in streptozotocin (STZ)-induced diabetic mice and whether its influence can be prevented by aminoguanidine (AG), a specific iNOS inhibitor. Twenty-four male mice were divided into four study groups (n=6) receiving a daily dose of 100 mg.kg-1 AG for 90 days (Group AG), a single dose of 150 mg.kg-1 STZ (Group STZ), a single dose of 150 mg.kg-1 STZ followed by daily administration of 100 mg.kg-1 AG for 90 days (Group STZ-AG), and intraperitoneally injections of saline only (Group Control) for 90 days. Dispersion of NADPH-diaphorase (NADPH-d) was stronger in the kidney sections of STZ-treated animals compared with the controls. STZ treatment caused disruption of continuity of the brush borders in proximal tubules, glomerular endothelial damage, and considerable renin granules in the juxtaglomerular cells. AG administration following STZ treatment partly prevented histological and cytological changes in kidney cortex, and renin dispersion was similar to that in control animals. We found that increased inducible nitric oxide (iNO) caused kidney tissue degeneration that could be prevented to some extent by AG treatment. There is a possible relationship between increased iNOS and dispersion of renin granules in juxtaglomerular cells in diabetes.
本研究旨在探讨活化的诱导型一氧化氮合酶(iNOS)和一氧化氮(NO)对链脲佐菌素(STZ)诱导的糖尿病小鼠肾脏组织的影响,以及iNOS特异性抑制剂氨基胍(AG)是否可以预防其影响。24只雄性小鼠被分为4个研究组(n=6),每天服用100 mg。kg-1 AG治疗90天(AG组),单次剂量150 mg。kg-1 STZ (STZ组),单次剂量150 mg。kg-1 STZ,然后每日给药100 mg。STZ-AG组腹腔注射生理盐水90 d,对照组腹腔注射生理盐水90 d。与对照组相比,stz处理的动物肾脏组织中NADPH-diaphorase (NADPH-d)的弥散性更强。STZ治疗导致近端小管刷状边界连续性中断,肾小球内皮损伤,肾小球旁细胞出现大量肾素颗粒。STZ治疗后给予AG部分阻止肾皮质组织学和细胞学改变,肾素弥散与对照动物相似。我们发现,诱导型一氧化氮(iNO)升高可引起肾组织变性,而AG处理可在一定程度上预防这种变性。糖尿病肾小球旁细胞中iNOS的增加与肾素颗粒的分散可能有关。
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引用次数: 2
Quality of drinking water of Canakkale Province, Turkey 土耳其恰纳卡莱省饮用水质量
Pub Date : 2012-06-27 DOI: 10.18478/IUFSJB.07608
M. Coşkun, A. Çayır, M. Coşkun, Aslı Kovanci
Concentrations of arsenic cadmium, copper, lead, and zinc in the first flush and post-first flush of tap water were determined. Further, the tap water was allowed to run at least 3 minutes and samples were analyzed for bacteriological quality. The analysis showed that Cd and Pb concentrations in the first and second forty samples collected were under the detection limits of the analytical method employed. The concentration ranges of As, Cu, and Zn in the first forty samples were 0.95-11.9 µg/L, 0.011-0.58, and 0.04-5.29 mg/L, respectively. Lower ranges of Cu and Zn levels of 0.010-0.343 and 0.008-4.546 mg/L, respectively, were found in the second forty samples. Total aerobic mesophilic bacteria, total coliform and Escherichia coli were detected in 28, 18 and 1 out of 40 water samples, respectively. Our results show that some of the water samples that contained high levels of bacterial colonies were collected from four buildings, situated at two faculties of the university, a cafeteria and a bakery. These findings indicate that the distribution system and drinking water of Canakkale needs improvement.
测定了第一次冲洗和第一次冲洗后的自来水中砷、镉、铜、铅和锌的浓度。此外,让自来水至少运行3分钟,并对样本进行细菌质量分析。结果表明,第1、2 40份样品的Cd、Pb含量均在该方法的检出限之内。前40份样品中As、Cu、Zn的浓度范围分别为0.95 ~ 11.9µg/L、0.011 ~ 0.58µg/L和0.04 ~ 5.29 mg/L。另外40份样品的Cu和Zn含量分别为0.010 ~ 0.343 mg/L和0.008 ~ 4.546 mg/L。40份水样中检出总需氧嗜中温菌28株,总大肠菌群18株,大肠杆菌1株。我们的研究结果表明,一些含有高水平菌落的水样是从位于大学两个院系、一家自助餐厅和一家面包店的四座建筑物中收集的。这些发现表明,恰纳卡莱的分配系统和饮用水需要改进。
{"title":"Quality of drinking water of Canakkale Province, Turkey","authors":"M. Coşkun, A. Çayır, M. Coşkun, Aslı Kovanci","doi":"10.18478/IUFSJB.07608","DOIUrl":"https://doi.org/10.18478/IUFSJB.07608","url":null,"abstract":"Concentrations of arsenic cadmium, copper, lead, and zinc in the first flush and post-first flush of tap water were determined. Further, the tap water was allowed to run at least 3 minutes and samples were analyzed for bacteriological quality. The analysis showed that Cd and Pb concentrations in the first and second forty samples collected were under the detection limits of the analytical method employed. The concentration ranges of As, Cu, and Zn in the first forty samples were 0.95-11.9 µg/L, 0.011-0.58, and 0.04-5.29 mg/L, respectively. Lower ranges of Cu and Zn levels of 0.010-0.343 and 0.008-4.546 mg/L, respectively, were found in the second forty samples. Total aerobic mesophilic bacteria, total coliform and Escherichia coli were detected in 28, 18 and 1 out of 40 water samples, respectively. Our results show that some of the water samples that contained high levels of bacterial colonies were collected from four buildings, situated at two faculties of the university, a cafeteria and a bakery. These findings indicate that the distribution system and drinking water of Canakkale needs improvement.","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75025077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Cathepsin B inhibition by Z-FA.FMK blocks TNF-a/D-GalN-induced oxidative damage through increasing antioxidant defence in the brain of mice Z-FA抑制组织蛋白酶B。FMK通过增加小鼠大脑的抗氧化防御来阻断TNF-a/ d - galn诱导的氧化损伤
Pub Date : 2012-01-01 DOI: 10.18478/IUFSJB.79872
S. Gezginci-Oktayoglu, Ismet Burcu Turkyilmaz, R. Yanardag, Ş. Bolkent
Cathepsin B is a cysteine lysosomal protease which takes place in many inflammatory diseases. Inflammatory processes within the brain represent a potential pathogenetic factor in neurodegenerative diseases and inhibiton of cathepsin B, which is an inflammation-related enzyme, can be a potential therapeutic utility in neuroinflammatory diseases. Thus, we researched the effect of benzyloxycarbonyl-phenilalaninealanine fluoromethylketone (Z-FA.FMK), which is a pharmacological inhibitor of cathepsin B, on tumour necrosis factor-I± (TNF-I±) and D-galactosamine (D-GalN)-induced brain damage. Because oxidative damage accompanies inflammatory processes we aimed to research the alteration in some markers of oxidative damage and antioxidant defence system. For this investigation mice were treated with 700 mg/kg D-GalN and 15 I¼g/kg TNF-I± one hour after administration with 8 mg/kg Z-FA.FMK. Treatment with Z-FA.FMK before TNF-I±/D-GalN injection resulted in decreased lipid peroxidation levels, while catalase, superoxide dismutase, glutathione peroxidase, paraoxonase 1 activities and glutathione levels were increased in the brain tissue of mice. These results showed that cathepsin B inhibition by Z-FA. FMK could be a potential therapeutic utility in neuroinflammatory diseases because of its ability to block TNF-I±/D-GalN-induced oxidative damage by increasing antioxidant defence in the brain of mice. Keywords: Benzyloxycarbonyl-Phenyl-Alanine-fluoromethylketone (Z-FA.FMK), Cathepsin B, Dgalactosamine, Oxidative damage, Tumour necrosis factor-I± (TNF-I±).
组织蛋白酶B是一种半胱氨酸溶酶体蛋白酶,在许多炎症性疾病中都有发生。脑内的炎症过程是神经退行性疾病的潜在致病因素,而组织蛋白酶B是一种与炎症相关的酶,抑制组织蛋白酶B可能是神经炎症疾病的潜在治疗工具。因此,我们研究了组织蛋白酶B的药理抑制剂苯氧羰基苯丙氨酸丙氨酸氟甲基酮(Z-FA.FMK)对肿瘤坏死因子- 1±(TNF-I±)和d -半乳糖胺(D-GalN)诱导的脑损伤的影响。由于氧化损伤伴随着炎症过程,我们旨在研究氧化损伤和抗氧化防御系统的一些标志物的改变。在给药1小时后,小鼠分别给予700 mg/kg D-GalN和15 μ g/kg TNF-I。Z-FA治疗。TNF-I±/D-GalN注射前FMK使小鼠脑组织脂质过氧化水平降低,过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、对氧磷酶1活性和谷胱甘肽水平升高。结果表明Z-FA对组织蛋白酶B有抑制作用。FMK可能是神经炎症疾病的潜在治疗工具,因为它能够通过增加小鼠大脑中的抗氧化防御来阻断TNF-I±/ d - galn诱导的氧化损伤。关键词:苯氧羰基苯基丙氨酸氟甲基酮(Z-FA.FMK),组织蛋白酶B,半乳糖胺,氧化损伤,肿瘤坏死因子- 1±(TNF-I±)
{"title":"Cathepsin B inhibition by Z-FA.FMK blocks TNF-a/D-GalN-induced oxidative damage through increasing antioxidant defence in the brain of mice","authors":"S. Gezginci-Oktayoglu, Ismet Burcu Turkyilmaz, R. Yanardag, Ş. Bolkent","doi":"10.18478/IUFSJB.79872","DOIUrl":"https://doi.org/10.18478/IUFSJB.79872","url":null,"abstract":"Cathepsin B is a cysteine lysosomal protease which takes place in many inflammatory diseases. Inflammatory processes within the brain represent a potential pathogenetic factor in neurodegenerative diseases and inhibiton of cathepsin B, which is an inflammation-related enzyme, can be a potential therapeutic utility in neuroinflammatory diseases. Thus, we researched the effect of benzyloxycarbonyl-phenilalaninealanine fluoromethylketone (Z-FA.FMK), which is a pharmacological inhibitor of cathepsin B, on tumour necrosis factor-I± (TNF-I±) and D-galactosamine (D-GalN)-induced brain damage. Because oxidative damage accompanies inflammatory processes we aimed to research the alteration in some markers of oxidative damage and antioxidant defence system. For this investigation mice were treated with 700 mg/kg D-GalN and 15 I¼g/kg TNF-I± one hour after administration with 8 mg/kg Z-FA.FMK. Treatment with Z-FA.FMK before TNF-I±/D-GalN injection resulted in decreased lipid peroxidation levels, while catalase, superoxide dismutase, glutathione peroxidase, paraoxonase 1 activities and glutathione levels were increased in the brain tissue of mice. These results showed that cathepsin B inhibition by Z-FA. FMK could be a potential therapeutic utility in neuroinflammatory diseases because of its ability to block TNF-I±/D-GalN-induced oxidative damage by increasing antioxidant defence in the brain of mice. Keywords: Benzyloxycarbonyl-Phenyl-Alanine-fluoromethylketone (Z-FA.FMK), Cathepsin B, Dgalactosamine, Oxidative damage, Tumour necrosis factor-I± (TNF-I±).","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76503261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of real-time PCR and conventional cultural methods to detect Escherichia coli O157:H7 in spices in Turkey 实时PCR与传统培养法检测土耳其香料中大肠杆菌O157:H7的比较
Pub Date : 2012-01-01 DOI: 10.18478/iufsjb.99956
Asli Ozkizilcik, M. Ateş, B. Çerçi
In this study, 66 different spice samples were analyzed in order to compare Real-Time PCR and conventional cultural methods for the detection of E. coli O157:H7 in spices. Thus, 66 different spice samples (raw materials) were collected from various places in Izmir, Turkey. Conventional cultural methods included pre-enrichment step, selection step and confirmation step. The molecular method included two steps: pre-enrichment and Real-Time PCR step. Beside these methods, total aerobic mesophilic bacteria, coliform, fecal coliform in samples, were also counted and the presence of Salmonella sp. was investigated in order to determine total microbial load in samples. The results showed that aerobic mesophilic bacteria counts were 5.1x103 -2.0x108 CFU/g, coliform counts were 8.0 x 102 CFU/g for the average values of all samples. Salmonella sp. was not found in any of the samples.In four samples E. coli O157:H7 positive by Real-Time PCR were found while all of the samples were negative by cultural methods. We observed that the results of Real-Time PCR were more reliable than conventional methods. Furthermore, the results were obtained in only 20 hours by Real-Time PCR method whereas conventional cultural method was completed in 4 days. Keywords: Cultural method, E. coli O157:H7, real-time PCR, spice
本研究分析了66种不同香料样品,比较了Real-Time PCR和传统培养方法对香料中大肠杆菌O157:H7的检测效果。因此,从土耳其伊兹密尔的不同地方收集了66种不同的香料样品(原材料)。传统的培养方法包括预富集步骤、选择步骤和确认步骤。分子法分为预富集和Real-Time PCR两步。除了这些方法外,还对样品中的好氧中温细菌、大肠菌群、粪便大肠菌群进行计数,并调查沙门氏菌的存在情况,以确定样品中的总微生物负荷。结果表明,所有样品的好氧中温细菌数平均值为5.1 × 103 -2.0 × 108 CFU/g,大肠菌群数平均值为8.0 × 102 CFU/g。在所有样本中均未发现沙门氏菌。在4份样本中,Real-Time PCR检测出大肠杆菌O157:H7阳性,而培养法检测均为阴性。我们观察到Real-Time PCR的结果比传统方法更可靠。此外,Real-Time PCR法仅需20小时即可获得结果,而常规培养方法需要4天才能完成。关键词:培养法,大肠杆菌O157:H7,实时PCR,香料
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引用次数: 0
Biofilm formation on galvanized steel by SRB isolate obtained from cooling tower water 从冷却塔水中提取的SRB分离物在镀锌钢板上形成生物膜
Pub Date : 2011-10-27 DOI: 10.18478/IUFSJB.45154
Derya Ozuolmez, A. Çotuk
In this study, we investigated biofilm formation on galvanized steel coupons by anaerobic bacteria isolate including sulphate-reducing bacteria (SRB) isolated from cooling tower water in a lab-scaled experimental setup. The test coupons were exposed to the culture of anaerobic bacteria isolate during 744 hours. In the course of time, anaerobic bacteria isolate could form biofilm on galvanized steel coupons. According to the statistical analyses, there was no significant difference between sessile and planktonic SRB counts, while a positive correlation was found out between SRB counts (P<0.01). Extracellular carbohydrate appeared to be degraded by bacteria culture in the test system. Keywords: Sulphate-reducing bacteria (SRB), cooling tower water, galvanized steel, biofilm.
在这项研究中,我们在实验室规模的实验装置中研究了从冷却塔水中分离的厌氧细菌(包括硫酸盐还原细菌(SRB))在镀锌钢板上形成的生物膜。试验片暴露于厌氧细菌分离物培养744小时。随着时间的推移,分离出的厌氧细菌可在镀锌钢板表面形成生物膜。经统计分析,固定式和浮游生物的SRB计数无显著差异,两者呈显著正相关(P<0.01)。细胞外碳水化合物似乎在测试系统中被细菌培养降解。关键词:硫酸盐还原菌,冷却塔水,镀锌钢,生物膜
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引用次数: 3
Presentation of flora, life forms and chorology of plant species in the Jahrom area (Fars Province, Iran) 伊朗法尔斯省Jahrom地区植物区系、生命形式和物种的时序介绍
Pub Date : 2011-10-27 DOI: 10.18478/IUFSJB.54441
G. Ghanbarian, E. Jafari, A. Hatami
In the present study, species were classified according to their growth habit and Raunkiaer's life- forms system were used to characterize the flora of Jahrom. Jahrom in the south east of the Fars Province is an area with about 1,627,470 hectares , maximum altitude at 3167 m and minimum altitude at 300 m, located between 52° 30I„ to 54° 00I„ east latitudes and 28 ° 00I„ to 29° 00I„north longitudes. We studied the area from a floristic and ecological point of view. The results showed that the flora of this region includes 346 species belonging to 234 genera and 67 families. Papilionaceae with 52 species, Asteraceae with 47 species and Poaceae with 36 species are the main families of this area. Plant life forms were studied by Raunkiaer's method. It showed that therophytes with (30.1%) of species, hemicryptophyte with (30.1%) of species, phanerophytes with (17.6%) of species and chamaephyte with (13%) of species, are the dominant life forms of the area. The chorological studies showed that (81.8%) of species belong to Irano-Turanian zone, (9.2%) of species belong to Sahara-Sindian zone and (9%) of common species belong to Irano-Turanian, Sahara-Sindian zones.
本研究根据植物的生长习性对其进行了分类,并采用Raunkiaer生命形式系统对其植物区系进行了表征。Jahrom位于法尔斯省东南部,面积约1,627,470公顷,最高海拔3167米,最低海拔300米,位于东纬52°30I "至54°00I ",北纬28°00I "至29°00I之间。我们从植物区系和生态的角度研究了这个地区。结果表明,该地区植物区系包括67科234属346种。该区主要科为凤蝶科52种,菊科47种,禾本科36种。用Raunkiaer的方法研究了植物的生命形式。结果表明,该地区的优势生物类型依次为食生植物(30.1%)、半隐生植物(30.1%)、显生植物(17.6%)和变色虫(13%)。古生物学研究表明:81.8%的物种属于伊朗-图兰带,9.2%的物种属于撒哈拉-辛地带,9%的常见物种属于伊朗-图兰带、撒哈拉-辛地带。
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引用次数: 5
期刊
IUFS Journal of Biology
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