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Flavonoids and some biological activities of Ailanthus excelsa leaves 臭椿叶类黄酮及其生物活性研究
Pub Date : 2010-11-25 DOI: 10.18478/IUFSJB.48603
A. Said, U. Hawas, S. El-Shenawy, S. M. Nofal, K. Rashed
In the present study, the methanolic extract of Ailanthus excelsa (Roxb) leaves, a plant used in Egyptian traditional medicine was investigated for phytochemical constituents and some biological activities. In vitro: antioxidant activity and in vivo: Lethal dose (LD 50 ) acute and chronic toxicity and anti-nociceptive and anti-inflammatory effects were determined. It was found that methanolic extract of Ailanthus excelsa leaves is nontoxic up to 5 g.kg -1 b. wt in mice. Also rats were given a daily single oral dose of the extract for 7 and 30 successive days for assessment of acute and chronic toxicity. The extract did not induce any significant change in serum levels of ALT, AST, GGT, total protein, albumin, BUN and creatinine as compared with saline control group in rats. The extract exhibited a significant antioxidant, anti-nociceptive activity and no anti-inflammatory effects. Chromatographic separation of A. excelsa MeOH extract has yielded eleven known flavonoid compounds. Their structures were established on the basis of chromatographic properties and spectroscopic (UV, 1 H, 13 C NMR, MS) analyses.
本研究以埃及传统药用植物Ailanthus excelsa (Roxb)叶片甲醇提取物为研究对象,对其化学成分和部分生物活性进行了研究。体外:测定抗氧化活性,体内:测定致死剂量(ld50)、急慢性毒性、抗伤害和抗炎作用。结果表明,臭椿叶甲醇提取物对小鼠的毒性可达5 g.kg -1 b. wt。大鼠每日口服单剂量提取物,连续7天和30天,以评估急性和慢性毒性。与生理盐水对照组相比,提取物对大鼠血清ALT、AST、GGT、总蛋白、白蛋白、BUN、肌酐水平无显著影响。提取物具有显著的抗氧化、抗伤害活性,但无抗炎作用。黄芪MeOH提取物的色谱分离得到了11种已知的类黄酮化合物。通过色谱性质和光谱(UV、1h、13c NMR、MS)分析确定了它们的结构。
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引用次数: 13
Morphological Characteristics and Growth Abilities of Free Living Amoeba Isolated From Domestic Tap Water Samples in İstanbul İstanbul生活自来水中分离的自由生活变形虫的形态特征和生长能力
Pub Date : 2010-11-24 DOI: 10.18478/IUFSJB.08200
Z. Zeybek, Miray Üstüntürk, Ali Rıza Binay
Free living amoeba (FLA) have been isolated from water and soil samples throughout the world. Among the many genera of FLA , members of only four genera are responsible for human disease: Acanthamoeba spp., Balamuthia mandrillaris , Naegleria fowleri and Sappinia diploidea . Naegleria spp. and Acanthamoeba spp. can be cultivated on nonnutrient agar (NNA) in the presence of living or dead bacteria. Balamuthia , however, will not grow with bacteria as a food source but they can feed upon smaller soil amoeba. In the current study we try to find out the morphological appearance (Trichrome staining) and growth characteristics of free living amoeba in different liquid culture media previously isolated from domestic tap water samples (10 isolates) and a strain of Acanthamoeba castellanii (A. castellanii) isolated from a keratitis case. Although A.castellanii strain grew on proteose peptone-yeast extract-glucose and proteose peptone-yeast (with or without antibiotics), our isolates from domestic tap water samples did not grow on any tested liquid culture media. It is suggested that investigating both the morphological characteristics and the growth characteristics on liquid culture media are necessary for the identification of free living amoeba.
自由生活的变形虫(FLA)已经从世界各地的水和土壤样品中分离出来。在FLA的许多属中,只有4个属的成员对人类疾病负责:棘阿米巴,曼氏巴拉姆西亚,福氏奈格里亚和Sappinia diploidea。Naegleria spp.和棘阿米巴spp.可以在非营养性琼脂(NNA)上培养,存在活的或死的细菌。然而,Balamuthia不会以细菌为食物来源,但它们可以以较小的土壤变形虫为食。在本研究中,我们试图了解从生活自来水样品(10株)和从角膜炎病例中分离的一株卡斯特兰棘阿米巴(a . castellanii)分离的不同液体培养基中自由活阿米巴的形态外观(三色染色)和生长特征。虽然castellanii菌株在蛋白酶-酵母提取物-葡萄糖和蛋白酶-酵母(含或不含抗生素)上生长,但我们从生活自来水样品中分离的菌株在任何测试的液体培养基上都没有生长。因此,研究其形态特征和在液体培养基上的生长特征是鉴定自由生活变形虫的必要条件。
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引用次数: 6
The Anti-Genotoxic Effect of Taurine on Aluminum Sulphate-Induced DNA Damage in Human Peripheral Lymphocytes 牛磺酸对硫酸铝诱导的人外周血淋巴细胞DNA损伤的抗基因毒性作用
Pub Date : 2010-11-24 DOI: 10.18478/IUFSJB.01865
H. Turkez, F. Geyikoğlu
Aluminum sulphate Al 2 (SO 4 ) 3 is commonly used as a coagulant in the purification of drinking water in many regions of the world. Aluminum (Al) is also involved in several pharmaceutical and cosmetic products. However, Al is suspected to cause serious disorders such as Alzheimer's by oxidative DNA damage. On the other hand, taurine (TA) is an amino acid found in mammalian tissues and it has been suggested to play a role in the defense against cellular damage. The objective of this study was to determine whether the supplemetation of a natural antioxidant, TA conferred the protection against Al exposure in human blood cultures. For this aim, sister chromatid exchange (SCE) and micronucleus (MN) rates were assessed. Present results showed that 10 μg/ml of Al 2 (SO 4 ) 3 has no effect on SCE and MN rates, but 20 μg/ml of this compound increased the rates of SCEs and MN. Besides, the TA at all studied concentrations (25, 50 and 100 μg/ml) did not alter the mean SCE and MN values as compared to controls. Moreover, the negative cytogenetic alterations induced by Al could be significantly ( P<0.05 ) reduced by the application of TA. This study reveals for the first time the ameliorative role of TA against Al-induced DNA damage in vitro .
硫酸铝al2 (so4) 3在世界许多地区被广泛用作饮用水净化的混凝剂。铝(Al)也涉及到一些医药和化妆品。然而,人们怀疑人工智能会通过DNA氧化损伤导致阿尔茨海默病等严重疾病。另一方面,牛磺酸(TA)是一种在哺乳动物组织中发现的氨基酸,它被认为在防御细胞损伤中发挥作用。本研究的目的是确定补充天然抗氧化剂TA是否能保护人体血液培养物免受Al暴露。为此目的,姊妹染色单体交换(SCE)和微核(MN)率进行了评估。结果表明,10 μg/ml的al2 (so4) 3对SCE和MN没有影响,而20 μg/ml的al2 (so4) 3对SCE和MN有促进作用。此外,与对照组相比,所有研究浓度(25、50和100 μg/ml)下的TA均未改变平均SCE和MN值。此外,施用TA可显著降低Al诱导的细胞遗传负性改变(P<0.05)。本研究首次揭示了TA在体外对al诱导的DNA损伤的改善作用。
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引用次数: 11
Cytohistological Studies During Cotton Somatic Embryogenesis With Brassinosteroid Application 油菜素内酯对棉花体细胞胚胎发生的细胞组织学研究
Pub Date : 2010-11-24 DOI: 10.18478/IUFSJB.01577
Y. Aydin, T. Talas-Oğraş, A. Altinkut, I. Ismailoğlu, E. Arican, N. Gozukirmizi
The histology and chromosome alterations of somatic embryo initiation and development in cotton ( Gossypium hirsutum L. cv. Coker) was examined during in vitro exogenous brassinosteroid (BR) application. Calli developed from hypocotyl explants were subjected to 0.5 μM BR. After 10 weeks of calli cultures with somatic embryos at different developmental stages were fixed for histological examinations. The histological examination showed that two types of embryogenic cells induced; epidermal or subepidermal. The embryogenic mass and somatic embryos were mostly derived from several morphologically competent cells from subepidermal cases; however single progenitor cells for epidermal situations. Chromosome behaviour of the cells surrounding embryogenic cells showed abnormal divisions and higher level of polyploidy on BR treated calli when compared to control group.
棉花体细胞胚发生和发育的组织学和染色体改变。在体外外源性油菜素内酯(BR)应用过程中检测Coker)。下胚轴外植体培养的愈伤组织经0.5 μM BR处理。不同发育阶段的体胚愈伤组织培养10周后进行组织学检查。组织学检查显示,诱导了两种类型的胚胎发生细胞;表皮的或皮下的胚性团块和体细胞胚多来源于表皮下病例的几个形态能态细胞;然而单祖细胞为表皮情况。BR处理的愈伤组织胚性细胞周围细胞的染色体行为表现为分裂异常,多倍体水平高于对照组。
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引用次数: 6
ENVIRONMENTAL BIOLOGY AND PATHOPHYSIOLOGY OF CADMIUM 镉的环境生物学和病理生理学
Pub Date : 2010-11-17 DOI: 10.18478/IUFSJB.63020
Y. Çotuk, M. Belivermiş, Ö. Kılıç
Cadmium (Cd) is a soft, silver-white metal being together with zinc and mercury in group II b in the periodic table. It's melting and boiling point are 320.9 0 C and 765 0 C, respectively. Cd is rapidly oxidized into cadmium oxide in the air. When reactive gases such as carbon dioxide, sulphur dioxide, sulphur trioxide and hydrogen chloride or water vapour are present in the air, cadmium vapour reacts to produce cadmium carbonate, cadmium sulphite, cadmium sulphate, cadmium chloride or cadmium hydroxide. These compounds may be formed in chimneys and emitted into the environment. It is indicated that zinc-bearing coals of the central United States and carboniferous age coals of other countries contain large sub economic resources of cadmium. Although some cadmium compounds such as acetate, chloride and sulphate are soluble in water; cadmium oxide, carbonate and sulphide are insoluble.
镉(Cd)是一种柔软的银白色金属,与锌和汞一起在元素周期表中属于b族。它的熔点和沸点分别是320.9℃和765℃。镉在空气中迅速氧化成氧化镉。当空气中存在二氧化碳、二氧化硫、三氧化硫和氯化氢或水蒸气等反应性气体时,镉蒸气会发生反应,产生碳酸镉、亚硫酸镉、硫酸镉、氯化镉或氢氧化镉。这些化合物可能在烟囱中形成并排放到环境中。结果表明,美国中部的含锌煤和其他国家石炭时代的煤含有大量的镉亚经济资源。虽然有些镉化合物如醋酸盐、氯化物和硫酸盐可溶于水;氧化镉、碳酸盐和硫化物是不溶的。
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引用次数: 11
EFFECT OF OVERIECTOMY AND OF ESTROGEN ADMINISTRATION UPON DUODENAL ULCERATION INDUCED BY CYSTEAMINE 半胱胺致十二指肠溃疡的十二指肠切除术及雌激素治疗效果
Pub Date : 2010-11-17 DOI: 10.18478/IUFSJB.75270
A. Kv, M. KuraneM, M. PillaiM.
Duodenal ulcers were induced in ovariectomized, intact and old mice using cysteamine hydrochloride. Under these experimental conditions ovariectomy and old age strikingly increased sensitivity to ulcer induction while estrogen administration showed a decrease in sensitivity to ulcer induction. Nevertheless, the administration of estrogen in old mice showed no effects in either intact or overiectomized mice. This change in ulcer sensitivity reflected from histological, histochemical and biochemical studies. The histological study was performed by using haematoxylin-eosin and haematoxyline staining technique. The histochemistry of the duodenal region was studied by using periodic acid Schiff reaction (PAS) for glycoproteins. The biochemical study was performed to study various constituents of glycoproteins like hexose, fucose, sialic acid and the protein. The result showed that ulcer severity was more in overiectomized cysteamine treated mice and old mice treated with cysteamine. The histological studies showed that ovariectomy decreased or did not show any change in the ulcer sensitivity considering cryptus Lieburkuhn and Brunner's glands. The same result reflected by differential intensity in the staining property of the Brunner's gland. The biochemical study showed that the glycoprotein contents were reduced many times in overiectomized cysteamine treated mice and their reversal in estrogen administered ovariectomized cysteamine injected mice. These findings prove that estrogen protects the duodenal ulcer from cysteamine administration.
用盐酸半胱胺对切除卵巢、完整卵巢和老龄小鼠进行十二指肠溃疡诱导。在这些实验条件下,卵巢切除术和老年显著增加对溃疡诱导的敏感性,而给予雌激素则显示对溃疡诱导的敏感性降低。然而,在老年小鼠中施用雌激素对完整或过度切除的小鼠均无影响。这种溃疡敏感性的变化反映在组织学、组织化学和生化研究中。采用苏木精-伊红和苏木精染色技术进行组织学研究。采用周期性酸性席夫反应(PAS)对十二指肠区糖蛋白进行组织化学检测。对糖蛋白的各种成分如己糖、焦糖、唾液酸和蛋白质进行了生化研究。结果表明,半胱胺过度切除小鼠和半胱胺治疗老年小鼠的溃疡严重程度更大。组织学研究显示卵巢切除术降低或未显示溃疡敏感性的变化,考虑到隐窝Lieburkuhn和Brunner腺体。布鲁纳腺染色性质的不同强度也反映了同样的结果。生化研究表明,半胱胺过度切除小鼠的糖蛋白含量降低了许多倍,而去卵巢半胱胺注射雌激素小鼠的糖蛋白含量则有所逆转。这些发现证明,雌激素可以保护十二指肠溃疡免受半胱胺的影响。
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引用次数: 4
Role of salt stress on seed germination and growth of jojoba plant Simmondsia chinensis (Link) Schneider 盐胁迫对荷荷巴种子萌发和生长的影响
Pub Date : 2010-11-02 DOI: 10.18478/IUFSJB.01476
A. Berrichi, Reda Tazi, Ahmed Belliro, N. Kouddane, A. Bouali
An extensive problem in agriculture is the accumulation of salts from irrigation water. Evaporation and transpiration remove pure water from the soil. This water loss concentrates solutes in the soil. When irrigation water contains a high concentration of solutes, salts can reach rapidly injurious levels vis-a-vis salt-sensitive species. One factor limiting jojoba plant ( Simmondsia chinensis (Link) Schneider) domestication in the oriental areas of Morocco is the damage caused by salt effect since irrigation enhances salinity in plantation soils. The objectives of this study were to define the effect of various NaCl concentration levels on plant growth and seed germination. The tolerance to salinity of jojoba seed and seedlings was assessed at NaCl concentrations of 0, 1, 2, 3, 5, and 7 g l −1 and the experiment was undertaken in the laboratory under greenhouse conditions. Results obtained show that more than half of the seeds germinated in the presence of 7g/l of NaCl; whereas 5g/l of NaCl inhibited completely the emergence of plumules. Concerning jojoba seedlings, 3 g/l of NaCl marked the start of negative affect on the growth.
农业中一个广泛存在的问题是灌溉水中盐分的积累。蒸发和蒸腾作用从土壤中除去纯水。这种失水使土壤中的溶质浓缩。当灌溉水含有高浓度的溶质时,相对于对盐敏感的物种,盐可以迅速达到有害的水平。荷荷巴(Simmondsia chinensis (Link) Schneider)在摩洛哥东部地区驯化的一个限制因素是盐效应造成的损害,因为灌溉提高了种植园土壤的盐度。本研究的目的是确定不同NaCl浓度对植物生长和种子萌发的影响。在实验室温室条件下,对NaCl浓度分别为0、1、2、3、5和7 g l−1的荷荷巴种子和幼苗的耐盐性进行了评价。结果表明,在7g/l NaCl浓度下,种子萌发率超过一半;而5g/l NaCl完全抑制了子体的产生。3 g/l NaCl对荷荷巴幼苗的生长开始产生负面影响。
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引用次数: 18
Isolation and identification of Two new cyclic amino acids from the seeds of Zizyphus spina-christi L. (Willd) by means of 1H-NMR-13C-NMR-HSQC-HMBC and GC-MS 采用1H-NMR-13C-NMR-HSQC-HMBC和GC-MS分离鉴定两种新的环氨基酸
Pub Date : 2010-11-02 DOI: 10.18478/IUFSJB.70605
A. Said, A. Huefner, E. Table, G. Fawzy
In this study we isolate two new cyclic amino acids from the seeds of Zizyphus spina-christi L. (Willd ) 70% methanolic extract. The two compounds were identified by means of 1 H-NMR- 13 C-NMR-HSQC-HMBC and GC-MS as 4-hydroxymethyl-1-methyl pyrrolidine-2-carboxylic acid (less polar and major compound) and 4-hydroxy-4-hydroxymethyl-1-methyl pyrrolidine-2-carboxylic acid (more polar and minor compound) of the ratio 4:3.
从70%甲醇提取物Zizyphus spina-christi L. (wild)中分离出两个新的环状氨基酸。通过1h - nmr - 13c - nmr - hsqc - hmbc和GC-MS分别鉴定为4-羟甲基-1-甲基吡咯烷-2-羧酸(极性较低,主化合物)和4-羟基-4-羟甲基-1-甲基吡咯烷-2-羧酸(极性较强,次化合物),比例为4:3。
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引用次数: 2
LENGTH-WEIGHT RELATIONSHIPS OF FISHES IN SHALLOW WATERS OF ERDEK BAY (SEA OF MARMARA, TURKEY) 土耳其马尔马拉海erdek湾浅水鱼类的长重关系
Pub Date : 2010-11-02 DOI: 10.18478/IUFSJB.34222
Çetin Keskin, Ö. Gaygusuz
The study was carried out monthly between April 2000 and December 2001 on the seagrass and/or sandy bottom in shallow waters of Erdek Bay (Sea of Marmara, Turkey). Samples were collected using 35 m long beach seine. Fish samples were identified, measured and weighed. The length-weight relationships of juvenile and adult specimens of 36 fish species were investigated. The parameters a and b were calculated by functional regression, as was the coefficient of determination ( r 2 ). r 2 values were higher than 0.950 for 27 species in the list, while only one species was lower than 0.80. The exponent b ranged from 2.282 to 3.741.
该研究于2000年4月至2001年12月期间每月在Erdek湾(土耳其马尔马拉海)浅水的海草和/或沙质底部进行。样本采用35米长滩围网采集。对鱼类样本进行了鉴定、测量和称重。研究了36种鱼类幼鱼和成鱼的长重关系。通过函数回归计算参数a和b,并计算决定系数(r2)。r2值大于0.950的有27种,小于0.80的只有1种。指数b的取值范围为2.282 ~ 3.741。
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引用次数: 39
First record of Amphorellopsis tetragona ( Protozoa: Ciliophora: Tintinnina)
Pub Date : 2010-11-02 DOI: 10.18478/IUFSJB.72728
Benin Toklu Alıçlı, N. Balkıs, A. Toklu, Ahmet Höbek
Amphorellopsis tetragona was reported for the first time from the Sea of Marmara and contributed to the regional check-list of the plankton species of Turkish seas. The specimen was collected on 17 October 2008 from surface neritic waters with a depth of 30m in the research area. The primary hydrographic parameters, temperature 17 oC, salinity 27 ppt and dissolved oxygen 5 mg l -1 , were recorded at the sampling station.
本研究首次在马尔马拉海报道了四角虾,为土耳其海域浮游生物种类区域核对表作出了贡献。该标本于2008年10月17日从研究区域30米深的浅海表层水域中采集。采样站记录了主要水文参数:温度17℃,盐度27 ppt,溶解氧5 mg l -1。
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引用次数: 2
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IUFS Journal of Biology
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