Pub Date : 2022-12-15DOI: 10.18231/j.ijmmtd.2022.056
Zahraa Al Mohsen, Mohammed A. Al Mufarah, Naif O. Alharbi, Anfal Y. Aqeeli, Rawan F. Bokhedher
One of the greatest causes of death in the globe is tobacco cigarette smoking. Smoking affects hematological markers both acutely and chronically. The current study's objective was to evaluate the severity of the negative effects of cigarette smoking on biochemical traits in healthy smokers. This study included 280 participants, only 56 smokers and 100 non-smokers. They were smoking for three years, the smokers regularly smoked 10 to 20 cigarettes per day. The fully automatic hematological analyzer CELL-DYN 3700 examined the total number of blood cells. White blood cells (p0,001), hemoglobin (p=0,042), mean corpuscular volume (p=0,001), and mean corpuscular hemoglobin concentration (p0,001) were all considerably greater in the smokers. No other measured metrics varied considerably from one another. Red blood cells, white blood cells, hemoglobin, hematocrit, and mean corpuscular hemoglobin were all significantly higher (p0,001) in male smokers compared to female smokers after smoking cigarettes.: Our study concluded that smoking cigarettes continuously has severe negative effects on hematological variables, including hemoglobin, white blood cell count, mean corpuscular volume, mean corpuscular hemoglobin concentration, red blood cell count, and hematocrit. These modifications may be linked to an increased risk of polycythemia vera, atherosclerosis, chronic obstructive pulmonary disease, and/or cardiovascular diseases.
{"title":"Consequence of smoking on hematological parameters in apparently healthy medical students","authors":"Zahraa Al Mohsen, Mohammed A. Al Mufarah, Naif O. Alharbi, Anfal Y. Aqeeli, Rawan F. Bokhedher","doi":"10.18231/j.ijmmtd.2022.056","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.056","url":null,"abstract":"One of the greatest causes of death in the globe is tobacco cigarette smoking. Smoking affects hematological markers both acutely and chronically. The current study's objective was to evaluate the severity of the negative effects of cigarette smoking on biochemical traits in healthy smokers. This study included 280 participants, only 56 smokers and 100 non-smokers. They were smoking for three years, the smokers regularly smoked 10 to 20 cigarettes per day. The fully automatic hematological analyzer CELL-DYN 3700 examined the total number of blood cells. White blood cells (p0,001), hemoglobin (p=0,042), mean corpuscular volume (p=0,001), and mean corpuscular hemoglobin concentration (p0,001) were all considerably greater in the smokers. No other measured metrics varied considerably from one another. Red blood cells, white blood cells, hemoglobin, hematocrit, and mean corpuscular hemoglobin were all significantly higher (p0,001) in male smokers compared to female smokers after smoking cigarettes.: Our study concluded that smoking cigarettes continuously has severe negative effects on hematological variables, including hemoglobin, white blood cell count, mean corpuscular volume, mean corpuscular hemoglobin concentration, red blood cell count, and hematocrit. These modifications may be linked to an increased risk of polycythemia vera, atherosclerosis, chronic obstructive pulmonary disease, and/or cardiovascular diseases.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83503853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.18231/j.ijmmtd.2022.057
Prajna Sharma, P. K, Sanchita Shettigar
: Dengue is currently the second most prevalent vector borne disease in the world. Dengue fever and its more serious forms, dengue hemorrhagic fever and dengue shock syndrome are becoming important public health problems. Since there is no immunoprophylaxis or specific antiviral therapy available, clinico-microbiological diagnosis plays a vital role in patient management and implementation of control measures. : To identify dengue seropositive patients by NS1 antigen and anti-dengue IgM antibody detection by ELISA and correlate the changes in epidemiology. A retrospective study was performed for a period of 5 years from January 2017 to December 2021 in A.J Institute of Medical Sciences & Research Centre, Mangaluru. 1871 seropositive cases of all age groups admitted in the medical wards were included. Rapid immunochromatography test and ELISA was performed to detect NS1 antigen and IgM antibodies. Platelet count and total leucocyte count were also analysed. : Out of 14656, 1871 samples (12.75%) were positive for dengue. Higher rate of cases were in males and the age group of 21 to 30 years were chiefly affected. 1158(61.89%) were positive for NS1 antigen and total positive cases for IgM antibody was 530(28.32%). About 183 (9.79%) cases were positive for both NS1 antigen and IgM antibody. An increase in the prevalence of dengue was recorded during the month of June to October. The present study indicates a decline in dengue infection from 2020 which may be attributable to preventive and control measures provided by the health care workers besides having increased awareness among people. The decline may also may be due to the outbreak of SARS –Cov-2 in December 2019, which led to under diagnosis of dengue cases.
{"title":"Trends in dengue virus infection with seasonal variation at a tertiary care centre, Mangaluru: A retrospective study","authors":"Prajna Sharma, P. K, Sanchita Shettigar","doi":"10.18231/j.ijmmtd.2022.057","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.057","url":null,"abstract":": Dengue is currently the second most prevalent vector borne disease in the world. Dengue fever and its more serious forms, dengue hemorrhagic fever and dengue shock syndrome are becoming important public health problems. Since there is no immunoprophylaxis or specific antiviral therapy available, clinico-microbiological diagnosis plays a vital role in patient management and implementation of control measures. : To identify dengue seropositive patients by NS1 antigen and anti-dengue IgM antibody detection by ELISA and correlate the changes in epidemiology. A retrospective study was performed for a period of 5 years from January 2017 to December 2021 in A.J Institute of Medical Sciences & Research Centre, Mangaluru. 1871 seropositive cases of all age groups admitted in the medical wards were included. Rapid immunochromatography test and ELISA was performed to detect NS1 antigen and IgM antibodies. Platelet count and total leucocyte count were also analysed. : Out of 14656, 1871 samples (12.75%) were positive for dengue. Higher rate of cases were in males and the age group of 21 to 30 years were chiefly affected. 1158(61.89%) were positive for NS1 antigen and total positive cases for IgM antibody was 530(28.32%). About 183 (9.79%) cases were positive for both NS1 antigen and IgM antibody. An increase in the prevalence of dengue was recorded during the month of June to October. The present study indicates a decline in dengue infection from 2020 which may be attributable to preventive and control measures provided by the health care workers besides having increased awareness among people. The decline may also may be due to the outbreak of SARS –Cov-2 in December 2019, which led to under diagnosis of dengue cases.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83882797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.18231/j.ijmmtd.2022.067
Anupam Das, M. Sen, P. Pandey, Apurva Rautela, Haniya Jafar, Vikram Singh, Jaya Garg, J. Agarwal
Malaria is a parasitic disease caused by and is associated with acute febrile episodes. The disease burden is highest in Southeast Asia. Various measures are being taken to curb the disease in different countries. Even after the introduction of various programmes there has been reports of imported malaria which has been transported from highly endemic countries to less endemic or countries not having cases of Malaria. Not only this, various other viral disease come in as coinfection with malaria which makes the situation even worse. Here we describe one such case of malaria imported from traveller from Nigeria to India who also developed coinfection with Chikungunya.
{"title":"Imported malaria with chikungunya co-infection: A case report","authors":"Anupam Das, M. Sen, P. Pandey, Apurva Rautela, Haniya Jafar, Vikram Singh, Jaya Garg, J. Agarwal","doi":"10.18231/j.ijmmtd.2022.067","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.067","url":null,"abstract":"Malaria is a parasitic disease caused by and is associated with acute febrile episodes. The disease burden is highest in Southeast Asia. Various measures are being taken to curb the disease in different countries. Even after the introduction of various programmes there has been reports of imported malaria which has been transported from highly endemic countries to less endemic or countries not having cases of Malaria. Not only this, various other viral disease come in as coinfection with malaria which makes the situation even worse. Here we describe one such case of malaria imported from traveller from Nigeria to India who also developed coinfection with Chikungunya.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85815170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.18231/j.ijmmtd.2022.050
R. Jakribettu, Nayan Anjan, Vidya Rao S, K. Binub, Preethy Edavaloth
Methicillin resistant Staphylococcus aureus is the common pathogen worldwide. It requires prolonged therapy to eradicate MRSA from a patient. This study was undertaken to determine the changing trend of antimicrobial resistance in MRSA over six years in a tertiary care center. : This was a retrospective observational study done at the tertiary care teaching hospital. The antibiotic sensitivity pattern of the MRSA isolated from various clinical samples from January 2013–December 2018 was studied. The data collected was entered in the Microsoft excel 2007, coded and were analyzed by frequency and percentage. During the study period from January 2013 to December 2018, 1006 (27.42%) patients had MRSA out of 3668 patients diagnosed with Staphylococcus aureus infection. Almost equal gender distribution was observed with males (52.78%), geriatric patients, i.e., ≥61 years constituted for around 32% infected patients. The samples that were received during this period included pus (64.59%), blood (26.23%), urine (5.53%) and sputum (3.65%). Other anti-MRSA drugs had changing resistance rate on year to year basis. Continuous monitoring of antibiotic sensitivity and rationalizing the use of antibiotics remain an important and effective strategy to minimize the emergence of multi-drug resistant MRSA strains.
{"title":"Study of antibiotic resistance pattern in Methicillin-resistant Staphylococcus aureus: A six year analysis","authors":"R. Jakribettu, Nayan Anjan, Vidya Rao S, K. Binub, Preethy Edavaloth","doi":"10.18231/j.ijmmtd.2022.050","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.050","url":null,"abstract":"Methicillin resistant Staphylococcus aureus is the common pathogen worldwide. It requires prolonged therapy to eradicate MRSA from a patient. This study was undertaken to determine the changing trend of antimicrobial resistance in MRSA over six years in a tertiary care center. : This was a retrospective observational study done at the tertiary care teaching hospital. The antibiotic sensitivity pattern of the MRSA isolated from various clinical samples from January 2013–December 2018 was studied. The data collected was entered in the Microsoft excel 2007, coded and were analyzed by frequency and percentage. During the study period from January 2013 to December 2018, 1006 (27.42%) patients had MRSA out of 3668 patients diagnosed with Staphylococcus aureus infection. Almost equal gender distribution was observed with males (52.78%), geriatric patients, i.e., ≥61 years constituted for around 32% infected patients. The samples that were received during this period included pus (64.59%), blood (26.23%), urine (5.53%) and sputum (3.65%). Other anti-MRSA drugs had changing resistance rate on year to year basis. Continuous monitoring of antibiotic sensitivity and rationalizing the use of antibiotics remain an important and effective strategy to minimize the emergence of multi-drug resistant MRSA strains.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88843873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.18231/j.ijmmtd.2022.042
Girish Narayanaswamy, Mahesh Babu Kothapalli, L. Rani, Uma Maheshwar, R. Babu, Sanjay Paruchuri, Shashidhar Karpuranath, Rama Mishra, S. Alapaty
Prevention of hospital-acquired infections (HAI) is central to providing safe and high quality healthcare. Transmission of infection between patients by health workers, and the irrational use of antibiotics have been identified as preventable aetiological factors for HAIs. Few studies have addressed this in developing countries.To assess the effectiveness of a multifaceted infection control and antibiotic stewardship programme on HAIs and antibiotic use. A retrospective study was conducted for a study period of 11months (June 2021-April 2022) in Vydehi Hospital, Bangalore. All patients admitted to the intensive care unit and wards were included in the study. Intervention period was 6 months (June 2021-Nov 2021) and post-intervention period was 5 months (Dec 2021- April 2022). Assessment of HAIs was made based on the criteria from the Centers for Disease Control and Prevention. The multifaceted intervention consisted of hand hygiene campaign, isolation of multidrug resistance organism’s patients, water and air quality analysis, training of health care workers in infection control practices, and antibiotic stewardship. Data was collected using an identical method in the intervention and post intervention periods.We observed a major reduction in HAIs, from 89% (198/222 patients) in the intervention period to 10.8% (24/222 patients) in the post intervention period (relative risk (RR) (95% CI) 0.48 (0.31 to 0.56). Antibiotic use in ICUs declined from 58% (780/1347) to 44% (442/995) (RR 0.44 (0.40 to 0.55). Overall, hand hygiene compliance among the health- care workers was maintained at 100% during both the periods. Multifaceted infection control interventions are effective in reducing HAI rates, improving the rational use of antibiotics, increasing hand hygiene compliance, and may reduce mortality in hospitalised patients in developing countries.
{"title":"Reducing hospital-acquired infections and improving the rational use of antibiotics in a tertiary care hospital in Bangalore","authors":"Girish Narayanaswamy, Mahesh Babu Kothapalli, L. Rani, Uma Maheshwar, R. Babu, Sanjay Paruchuri, Shashidhar Karpuranath, Rama Mishra, S. Alapaty","doi":"10.18231/j.ijmmtd.2022.042","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.042","url":null,"abstract":"Prevention of hospital-acquired infections (HAI) is central to providing safe and high quality healthcare. Transmission of infection between patients by health workers, and the irrational use of antibiotics have been identified as preventable aetiological factors for HAIs. Few studies have addressed this in developing countries.To assess the effectiveness of a multifaceted infection control and antibiotic stewardship programme on HAIs and antibiotic use. A retrospective study was conducted for a study period of 11months (June 2021-April 2022) in Vydehi Hospital, Bangalore. All patients admitted to the intensive care unit and wards were included in the study. Intervention period was 6 months (June 2021-Nov 2021) and post-intervention period was 5 months (Dec 2021- April 2022). Assessment of HAIs was made based on the criteria from the Centers for Disease Control and Prevention. The multifaceted intervention consisted of hand hygiene campaign, isolation of multidrug resistance organism’s patients, water and air quality analysis, training of health care workers in infection control practices, and antibiotic stewardship. Data was collected using an identical method in the intervention and post intervention periods.We observed a major reduction in HAIs, from 89% (198/222 patients) in the intervention period to 10.8% (24/222 patients) in the post intervention period (relative risk (RR) (95% CI) 0.48 (0.31 to 0.56). Antibiotic use in ICUs declined from 58% (780/1347) to 44% (442/995) (RR 0.44 (0.40 to 0.55). Overall, hand hygiene compliance among the health- care workers was maintained at 100% during both the periods. Multifaceted infection control interventions are effective in reducing HAI rates, improving the rational use of antibiotics, increasing hand hygiene compliance, and may reduce mortality in hospitalised patients in developing countries.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83547556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.18231/j.ijmmtd.2022.043
Kaina Bhonsle, A. Vyas, H. Vyas, Abhiraj, K. Hemwani
Urinary tract infection is most frequently found bacterial infection in urinary tract of human beings and 75-95% of urinary tract infections are caused by uropathogenic . The uropathogenic bacteria produces several virulence factors, siderophore is one of them. Siderophore is iron acquisition protein and play significant role in pathogenicity of UTI’s by helping uropathogenic bacteria to persist in urinary tract of host cells in adverse conditions. The study was conducted at SRL laboratory in Ujjain Center from November 2018- October 2019. Midstream urine sample of suspected UTI patients were collected and was isolated and identified using standard microbiological procedure and after incubation, growth was observed. Colonies more than 10cfu/ml were considered as significant for UTI and detection of siderophore production in uropathogenic bacteria was done by using Chrome Azurol Assay (CAS). Out of 200 collected urine sample in 120 urine samples uropathogenic bacteria was identified and isolated. Among 120 isolates of siderophore production was observed in 48 isolates of , and their percentage was 40%. Iron acquisition protein or siderophores are a major virulence factor necessary for pathogenesis of UTI caused by and CAS assay is the most efficient method for detection of siderophore production in uropathogenic
{"title":"Detection of siderophore production in uropathogenic Escherichia coli causing urinary tract infection in patients of Ujjain M.P. (India)","authors":"Kaina Bhonsle, A. Vyas, H. Vyas, Abhiraj, K. Hemwani","doi":"10.18231/j.ijmmtd.2022.043","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.043","url":null,"abstract":"Urinary tract infection is most frequently found bacterial infection in urinary tract of human beings and 75-95% of urinary tract infections are caused by uropathogenic . The uropathogenic bacteria produces several virulence factors, siderophore is one of them. Siderophore is iron acquisition protein and play significant role in pathogenicity of UTI’s by helping uropathogenic bacteria to persist in urinary tract of host cells in adverse conditions. The study was conducted at SRL laboratory in Ujjain Center from November 2018- October 2019. Midstream urine sample of suspected UTI patients were collected and was isolated and identified using standard microbiological procedure and after incubation, growth was observed. Colonies more than 10cfu/ml were considered as significant for UTI and detection of siderophore production in uropathogenic bacteria was done by using Chrome Azurol Assay (CAS). Out of 200 collected urine sample in 120 urine samples uropathogenic bacteria was identified and isolated. Among 120 isolates of siderophore production was observed in 48 isolates of , and their percentage was 40%. Iron acquisition protein or siderophores are a major virulence factor necessary for pathogenesis of UTI caused by and CAS assay is the most efficient method for detection of siderophore production in uropathogenic","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"117 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76869696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.18231/j.ijmmtd.2022.051
Ramakrishna Pai Jakribetu, T. George, M. Pais, P. D’silva, S. Natarajan, M. Baliga
Dengue, neglected tropical disease, caused by a RNA virus belonging to Flaviviridae family. Comorbid conditions like diabetes mellitus, can lead to severe dengue leading to higher morbidity and mortality. This study was undertaken to compare the clinical and laboratory profile of dengue patients with and without diabetes mellitus. A retrospective study conducted at the tertiary care hospital. The clinical and laboratory details of the adult patients diagnosed with dengue from January 2014 to March 2019 were included in the studied. The data was entered in Microsoft excel and analyzed. We have analyzed the hematological, hepatic and renal parameters in three groups: healthy individuals (n= 80), patients with diabetes (n= 66) and dengue (n= 422). Among the patients diagnosed with dengue, 393/422 (93. 12%) survived. Among the dengue patients who were diabetic (129), 115 (89. 14%) survived and 14 (10. 85%) succumbed. The most common symptoms in dengue patients were fever (100%), vomiting (53. 32%) and body ache (51. 9%). In deceased patients, symptoms of severe dengue like reduced urine output, bleeding manifestations, pedal oedema, altered sensorium were noted. Signs of fluid leak like ascites and pleural effusion were higher in deceased patients. Among the deceased patients, anaemia, thrombocytopenia, increased renal parameters were observed in diabetic patients. Dengue in diabetic patients is associated with anemia, thrombocytopenia and higher mortality. There is no significant difference in hepatic and renal parameters in survived dengue diabetic and non-diabetic patients, but deceased patients had significant thrombocytopenia and increased renal parameters.
{"title":"Comparative study of Clinicolaboratory parameters of dengue in diabetic and non-diabetic from a tertiary care hospital","authors":"Ramakrishna Pai Jakribetu, T. George, M. Pais, P. D’silva, S. Natarajan, M. Baliga","doi":"10.18231/j.ijmmtd.2022.051","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.051","url":null,"abstract":"Dengue, neglected tropical disease, caused by a RNA virus belonging to Flaviviridae family. Comorbid conditions like diabetes mellitus, can lead to severe dengue leading to higher morbidity and mortality. This study was undertaken to compare the clinical and laboratory profile of dengue patients with and without diabetes mellitus. A retrospective study conducted at the tertiary care hospital. The clinical and laboratory details of the adult patients diagnosed with dengue from January 2014 to March 2019 were included in the studied. The data was entered in Microsoft excel and analyzed. We have analyzed the hematological, hepatic and renal parameters in three groups: healthy individuals (n= 80), patients with diabetes (n= 66) and dengue (n= 422). Among the patients diagnosed with dengue, 393/422 (93. 12%) survived. Among the dengue patients who were diabetic (129), 115 (89. 14%) survived and 14 (10. 85%) succumbed. The most common symptoms in dengue patients were fever (100%), vomiting (53. 32%) and body ache (51. 9%). In deceased patients, symptoms of severe dengue like reduced urine output, bleeding manifestations, pedal oedema, altered sensorium were noted. Signs of fluid leak like ascites and pleural effusion were higher in deceased patients. Among the deceased patients, anaemia, thrombocytopenia, increased renal parameters were observed in diabetic patients. Dengue in diabetic patients is associated with anemia, thrombocytopenia and higher mortality. There is no significant difference in hepatic and renal parameters in survived dengue diabetic and non-diabetic patients, but deceased patients had significant thrombocytopenia and increased renal parameters.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84249915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.18231/j.ijmmtd.2022.045
Ushangishvili Mg, S. P
: Vaginitis is the most common health problem among women of reproductive age group, which is associated with gynaecological and obstetrical complications and also increases the transmission of HIV and other sexually transmitted diseases.: This study aimed at estimating the various etiological agents causing vaginitis, different laboratory methods for its diagnosis, isolation of different species and antibiotic susceptibility pattern of aerobic bacterial isolates.: In present study, 104 clinically suspected cases of vaginitis attending gynaecology OPD was included after obtaining informed consent and various data were collected using via questionnaire. Three high vaginal swabs collected from each patient and subjected to PH, Whiff test, Microscopic examination (Grams staining, KOH mount, Acridine orange staining (AO), Saline wet mount) Culture and Biochemical reactions following standard protocols. Aerobic isolates were subjected to antibiotic susceptibility testing as per CLSI guidelines.: Out of 104 Clinically suspected cases most common type of vaginitis were Bacterial vaginosis (BV)51.9% followed by Candidiasis 41.3%, Trichomoniasis 3.9%. Most common species isolated in vulvovaginal Candidiasis is 32.5%. Majority of Gram positive organisms were susceptible to Linezolid and Gram negative organisms to Azithromycin.: Bacterial vaginosis, Candidiasis and Trichomoniasis are common problem in women of reproductive age, therefore screening of vaginal infection in women of reproductive age should be implemented. Culture and sensitivity should be mandatory and treatment should be based on invitro susceptibility testing. So that misuse of antibiotics will be avoided.
{"title":"Microbiological study of vaginitis among women of reproductive age group","authors":"Ushangishvili Mg, S. P","doi":"10.18231/j.ijmmtd.2022.045","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.045","url":null,"abstract":": Vaginitis is the most common health problem among women of reproductive age group, which is associated with gynaecological and obstetrical complications and also increases the transmission of HIV and other sexually transmitted diseases.: This study aimed at estimating the various etiological agents causing vaginitis, different laboratory methods for its diagnosis, isolation of different species and antibiotic susceptibility pattern of aerobic bacterial isolates.: In present study, 104 clinically suspected cases of vaginitis attending gynaecology OPD was included after obtaining informed consent and various data were collected using via questionnaire. Three high vaginal swabs collected from each patient and subjected to PH, Whiff test, Microscopic examination (Grams staining, KOH mount, Acridine orange staining (AO), Saline wet mount) Culture and Biochemical reactions following standard protocols. Aerobic isolates were subjected to antibiotic susceptibility testing as per CLSI guidelines.: Out of 104 Clinically suspected cases most common type of vaginitis were Bacterial vaginosis (BV)51.9% followed by Candidiasis 41.3%, Trichomoniasis 3.9%. Most common species isolated in vulvovaginal Candidiasis is 32.5%. Majority of Gram positive organisms were susceptible to Linezolid and Gram negative organisms to Azithromycin.: Bacterial vaginosis, Candidiasis and Trichomoniasis are common problem in women of reproductive age, therefore screening of vaginal infection in women of reproductive age should be implemented. Culture and sensitivity should be mandatory and treatment should be based on invitro susceptibility testing. So that misuse of antibiotics will be avoided.","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86550741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.18231/j.ijmmtd.2022.037
Bodhankar M G
{"title":"New perspectives for exploring Medical Microbiology and Tropical Diseases","authors":"Bodhankar M G","doi":"10.18231/j.ijmmtd.2022.037","DOIUrl":"https://doi.org/10.18231/j.ijmmtd.2022.037","url":null,"abstract":"","PeriodicalId":14553,"journal":{"name":"IP International Journal of Medical Microbiology and Tropical Diseases","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89083715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-15DOI: 10.18231/j.ijmmtd.2022.047
Aarushi Mangla, Hetal Vasani, M. Vegad
Metallo-beta-lactamase (MBL) producing Pseudomonas is a worrisome pathogen to hospital infection control due to production of resistance to multiple antibiotics. So, treatment options are narrowed down to only few antibiotics which will result in high morbidity and mortality. This study was conducted with the aim to detect prevalence of MBL producing Pseudomonas aeruginosa and to compare the different phenotypic methods for the detection of MBL production in Imipenem resistant clinical isolates of Pseudomonas aeruginosa. : In this prospective study total of 14,145 different clinical samples received from different wards. Out of which 804 Pseudomonas aeruginosa were isolated and antibiotic sensitivity testing was done by Kirby-Bauer disc diffusion method using CLSI guidelines. These were evaluated for Carbapenems resistance and MBL production. Imipenem resistant strains were subjected to screening tests like Double-disc synergy test, Combined disc diffusion test (CDT) and Modified-Hodge test. : In our study out of 804 isolates,153(19.02%) isolates are resistant to Imipenem. Out of 153 Imipenem resistant isolates, Combined disc diffusion test was positive in 100%, Double disc synergy test in 76.5% and Modified-hodge test in 84.15% isolates.: Combined disc diffusion test is very sensitive, cost effective and convenient screening test for detection of MBL producing Pseudomonas aeruginosa. So, all Imipenem resistant Pseudomonas isolates should be regularly screened for detection of MBL by Combined disc diffusion test (CDT) to prevent spread of resistance, longer hospital stay and treatment failure.
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