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“Shi-Kan” on Ecotoxicology 生态系统的“shikan”
Pub Date : 2007-06-30 DOI: 10.11403/JSET.10.11
I. Aoyama
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引用次数: 0
Assessment of overall herbicide effects on river ecosystems through periphyton and aquatic plants 通过周围植物和水生植物评价除草剂对河流生态系统的总体影响
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.51
S. Hatakeyama
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引用次数: 0
Pollution by estrogens in river and estuarine waters around Kuala Lumpur, Malaysia, and their effects on the estuarine Java-medaka, Oryzias javanicus 马来西亚吉隆坡周边河流和河口水域雌激素污染及其对河口爪哇的影响
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.141
J. Koyama, Shoko Imai, K. Fujii, S. Kawai, C. Yap, A. Ismail
Estrogens (17 B-estradiol and estrone) concentrations in river and estuarine waters around Kuala Lumpur, Malaysia were determined by enzyme-linked immunosorbent assay while estrogenic potential of the waters were ascertained by three in vitro bioassays, namely E-screen, Ishikawa cell-alkaline phosphatase and yeast estrogen screen. Moreover, hepatic vitellogenin, a precursor of yolk protein that is specific to females, were examined in male and female Java-medaka collected in the same area. 17B-estradiol and estrone concentrations were less than 6.1 ng/L and 127 ng/L, respectively. The highest 17B-estradiol equivalent activity, 284 ng/L, was detected by in vitro assay in Sungai Kuyoh, which was adjacent to a sewage plant. 17B-estradiol and estrone concentrations were not equal to their estrogenic potentials determined by in vitro assay implying the presence of other estrogenic chemicals in these waters. Hepatic vitellogenin concentrations of both male and female java-medaka were the same to laboratory-cultured individuals. Although relatively high estrogen concentrations were detected in some waters of Malaysia, these were not high enough to induce hepatic vitellogenin in male fishes. Further research, however, is necessary to cover more number of sampling sites.
采用酶联免疫吸附法测定了马来西亚吉隆坡附近河流和河口水域的雌激素(17 b -雌二醇和雌酮)浓度,并采用E-screen、Ishikawa细胞碱性磷酸酶和酵母雌激素筛查三种体外生物测定法确定了该水域的雌激素潜能。此外,在同一地区收集的雄性和雌性爪哇鱼中检测了雌性特有的卵黄蛋白前体肝卵黄原蛋白。17b -雌二醇和雌酮浓度分别小于6.1 ng/L和127 ng/L。通过体外实验检测到Sungai Kuyoh的17b -雌二醇当量活性最高,为284 ng/L,邻近污水厂。b -雌二醇和雌酮的浓度不等于体外测定的雌激素潜力,这意味着这些水中存在其他雌激素化学物质。雄性和雌性水爪哇鱼的肝脏卵黄蛋白原浓度与实验室培养的个体相同。虽然在马来西亚的一些水域检测到相对较高的雌激素浓度,但这些浓度不足以诱导雄性鱼的肝卵黄蛋白原。然而,需要进一步的研究以覆盖更多的取样地点。
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引用次数: 10
Acute Toxicity of the Insecticide Diazinon and Carbaryl to Calanoid and Cyclopoid Copepoda (Eodiaptomus, Mesocyclops and Thermocyclops) in Different Life Stages 杀虫剂二嗪酮和西威尼对角足类和Cyclopoid桡足类(Eodiaptomus, mescyclops和Thermocyclops)不同生命阶段的急性毒性
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.133
Hirokazu Takahashi, Kwang‐Hyeon Chang, T. Hanazato
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引用次数: 2
Toxicity identification evaluation on industrial and municipal solid waste landfill leachates 工业和城市生活垃圾填埋场渗滤液的毒性鉴定评价
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.11
Lei Lei, M. Piao, H. Nishizaki, Hirofumi Nakamura, I. Aoyama
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引用次数: 3
Current environmental pesticide sciences with fish 当前环境农药科学与鱼类
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.69
S. Kashiwada
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引用次数: 0
Ecotoxicity testing of bioremediation agents for shoreline oil-spill clean up 岸线溢油清理生物修复剂的生态毒性试验
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.115
Tomoyasu Hirano, R. Masho, Daisuke Koizumi, S. Tabata, Kentaro Suzuki, Mai Sakabe, Y. Nakano, Nobuyuki Sato, R. Sudo
We present the results of a test run done for protocols drafted to evaluate the toxicity of different bioremediation agents against spilled-oil using marine organisms and crude oil. The aim of the study was to evaluate experimentally the change in toxicity of oil when mixed with bioremediation agents and fertilizers used on oiled shores to enhance oil biodegradation under actual environmental conditions prevailing at the oiled site before considering any large-scale application. The protocols were designed to use three marine species at different trophic levels, i.e. diatoms Skeletonema costatum, rotifers Brachionus plicatilis, and red sea bream Pagrus major, and to carry out algal growth inhibition, zooplankton acute immobilization, and fish acute toxicity tests. The agents tested are: Inipol EAP22, oleophilic fertilizer; Terrazyme, bioaugmentation agent; and Super IB/Linstar, slow-release fertilizer. While Super IB/Linstar did not enhance the toxicity of oil, clear suppression of diatom cell growth was observed when 100 ppm of Inipol EAP22 was added to oil. The concen-
我们提出了一项试验运行的结果,该试验是为评估不同生物修复剂对使用海洋生物和原油的溢油的毒性而起草的。该研究的目的是在考虑大规模应用之前,通过实验评估在受油海岸使用的生物修复剂和肥料混合后,在受油地点的实际环境条件下,石油的毒性变化,以加强石油的生物降解。该方案旨在使用三种不同营养水平的海洋物种,即硅藻骨骨藻、轮虫折臂轮虫和红鲷,并进行藻类生长抑制、浮游动物急性固定化和鱼类急性毒性试验。所测试的药剂有:inpol EAP22,亲油肥料;生物增强剂Terrazyme;Super IB/Linstar缓释肥料。虽然Super IB/Linstar对油的毒性没有增强作用,但当油中添加100 ppm的Inipol EAP22时,可以明显抑制硅藻细胞的生长。农用地-
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引用次数: 1
A Toxicogenomics Approach for Assessing Toxic Effects of POPs 评估持久性有机污染物毒性效应的毒物基因组学方法
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.81
K. Nakayama
1.POPs汚染の現状 残留性有機汚染物質(Persistent Organic Pollutants: POPs)はその毒性,難分解性,生物 蓄積性および長距離輸送性から,国際的に協調 しそれらの廃絶,削減を目指すため,「残留性 有機汚染物質に関するストックホルム条約」 (POPs条約)に基づいて,製造および使用が禁 止あるいは制限されている。同条約ではダイオ キシン類や PCB類をはじめ,農薬では殺虫剤と して使用されてきた DDT類やクロルデン,ディ ルドリン,エルドリン等を含む合わせてあわせ て 12種類の有機塩素化合物が対象とされ,2004 年に発効された。我が国においても上記有機塩 素化合物のモニタリング調査を継続的に行って おり,なかでもPCB類やDDT類は他の有機塩素 化合物と比較して貝類,魚類および鳥類から現 在でも高濃度および高頻度で検出されることが 確認されている。 さらに,有機塩素化合物を蓄積している生物 から臭素系難燃剤 やフッ素化合物 が同時 に検出されている。臭素系難燃剤やフッ素化合 物はその汚染が顕在化したのが比較的最近であ るため規制が遅れ,現在でもそれらの環境中濃 度は低減傾向にないか,場合によっては上昇傾 向にあるとさえ伝えられている。フッ素化合物 は包装材の被覆のみならず消火剤や殺虫剤とし ても使用された実績がある。臭素系難燃剤の一 部およびフッ素化合物のうちペルフルオロオク タノスルホン酸(PFOS)は POPsに追加指定さ れる予定である。 このように,POPsをはじめとした残留性の強 い化合物は,その使用が禁止あるいは規制され た現在でもなお,多様な生物種より検出されて いる。さらに,これら複数の化学物質が生物に 蓄積していることから,複合的な影響が懸念さ れる。
POPs污染现状持久性有机污染物(Persistent Organic Pollutants)POPs)因其毒性、难分解性、生物积累性以及长距离运输性,国际上为了消除和减少它们,制定了《关于持久性有机污染物的斯德哥尔摩公约》根据(POPs条约),禁止或限制制造和使用。该条约规定,以大菌素类和PCB类为首,在农药中包括作为杀虫剂使用的DDT类和氯丁酮、迪多林、艾德林等。对象包括12种有机氯化合物,2004年生效。我国也在持续进行上述有机盐化合物的监测调查,其中PCB类和DDT类与其他有机氯化合物相比,在贝类、鱼类和鸟类中得到了验证。即使在也被确认为高浓度和高频率的检测。此外,在积累有机氯化合物的生物中同时检测出溴类阻燃剂和氟化合物。溴类阻燃剂和氟化合物的污染是在最近才显现出来的,因此限制较晚,现在它们在环境中的浓度并没有降低的趋势,有时甚至有上升趋势。氟化合物不仅被用作包装材料,还被用作灭火剂和杀虫剂。溴类阻燃剂的一部分以及氟化合物中的全氟磺酸(PFOS)将被追加指定到POPs中。像这样,POPs等持久性强的化合物,即使在禁止或限制使用的现在,仍从多种生物种类中被检测出。另外,由于这多种化学物质在生物体内积累,因此令人担心会产生复合影响。
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引用次数: 0
Study of Indoor Air Pollution by Permethrin: Determination of Permethrin in Indoor Air and 3-phenoxybenzoic acid in Residents' Urine as an Exposure Index 氯菊酯对室内空气污染的研究:室内空气中氯菊酯和居民尿液中3-苯氧苯甲酸作为暴露指标的测定
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.31
H. Dai, F. Asakawa, F. Jitsunari
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引用次数: 1
Chemical toxicity of thorium in Saccharomyces cerevisiae 钍在酿酒酵母中的化学毒性
Pub Date : 2006-01-01 DOI: 10.11403/JSET.9.87
Satomi Mizukami-Murata, Y. Murata, H. Iwahashi
Thorium(Th),an actinoide element, is among the most common naturally-occurring radioactive materials found in the environment. Thorium was used as a radiographic contrast agent(thorotrast)from 1930 to 1955, and many studies on its effects on the human body have been reported. Once thorium is absorbed, the risk of cancer increases due to direct bombardment from alpha-particles with high linear energy transfer during thorium decay. Most of these reports focused on irradiation damage caused by long-term thorium exposure. In contrast, acute thorium toxicity derives more from thorium’s chemical reactivity than its radioactive decay. Here, we report the chemical toxicity of thorium using yeast DNA microarray analysis. Thorium treatment of yeast was found to induce 136 genes. Genes involved in“C-compound and carbohydrate metabolism”,“energy”,“cell rescue, defense and virulence”and“biogenesis of cellular component”were induced significantly by thorium treatment. These genes were further classified into the following processes: oxidative stress(7 genes),glycogen and trehalose metabolism(6 genes),and cell wall damage(10 genes).However, only one gene related to DNA damage was induced. These results indicate that thorium causes cell wall damage and induces oxidative stress, and suggest that in order to overcome oxidative stress, cells promote metabolism of energy reserves in the form of glycogen and trehalose.
钍(Th)是一种锕系元素,是在环境中发现的最常见的天然放射性物质之一。从1930年到1955年,钍被用作放射造影剂(thorotrast),并有许多关于其对人体影响的研究报道。一旦钍被吸收,癌症的风险增加,因为在钍衰变期间具有高线性能量转移的α粒子的直接轰击。这些报告大多集中在长期接触钍引起的辐射损伤。相比之下,急性钍毒性更多地来自钍的化学反应性,而不是其放射性衰变。在这里,我们使用酵母DNA芯片分析报告钍的化学毒性。用钍处理酵母可诱导136个基因。钍处理显著诱导了“c -化合物和碳水化合物代谢”、“能量”、“细胞救援、防御和毒力”以及“细胞组分生物发生”等相关基因。这些基因进一步分为氧化应激(7个基因)、糖原和海藻糖代谢(6个基因)和细胞壁损伤(10个基因)。然而,只诱导了一个与DNA损伤相关的基因。这些结果表明,钍可引起细胞壁损伤和氧化应激,并提示为了克服氧化应激,细胞促进糖原和海藻糖形式的能量储备代谢。
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引用次数: 5
期刊
Japanese journal of environmental toxicology
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