Pub Date : 2023-03-21DOI: 10.18500/1816-9775-2023-23-1-86-93
A. V. Simonenko, Grigoriy K. Savelyev, El-Khih Ayya Nidal, V. Khanadeev, E. Tuchina
Gold nanostars with an average core diameter of 122,2 nm and a spike length of 114,6 nm were synthesized and characterized at a concentration of 5,36×1010 pcs/ml with an absorption maximum of 840 nm. Gold nanostars were coated with thiolated polyethylene glycol, its amount was about 8×104 molecules per 1 particle and about 4,4×1015 molecules/ml in the colloid. The zeta potential of gold nanostars coated with PEG-SH was −2.3 mV. The combined effect of gold nanostars and low-intensity infrared (808 nm) laser radiation on the bacteria Staphylococcus aureus 209 P and Escherichia coli 113-13 has been studied. Incubation of suspensions of microorganisms in the presence of nanoparticles without access to light did not lead to a significant reduction in the number of bacteria. Irradiation for 30 min of bacterial suspensions containing nanoparticles caused the death of 39% of the S. aureus population and 80% of the E. coli population. During the thermometry of the studied suspensions, it was found that the temperature increase is dose-dependent. The increase in temperature of the control samples that did not contain photothermal agents did not exceed 1 °C in both cases throughout the entire time of the experiment. For suspensions of bacteria (equally S. aureus and E. coli) incubated with gold nanostars during irradiation, an increase in temperature indicators, on average, by 4 °C was revealed. The more pronounced antibacterial activity of the combination of gold nanostars and infrared (808 nm) laser radiation against E. coli can also be explained by the greater sensitivity of gram-negative microorganisms to photothermal exposure.
{"title":"Gold nanostars as a photoagent under the antimicrobial action of infrared (808 nm) laser radiation","authors":"A. V. Simonenko, Grigoriy K. Savelyev, El-Khih Ayya Nidal, V. Khanadeev, E. Tuchina","doi":"10.18500/1816-9775-2023-23-1-86-93","DOIUrl":"https://doi.org/10.18500/1816-9775-2023-23-1-86-93","url":null,"abstract":"Gold nanostars with an average core diameter of 122,2 nm and a spike length of 114,6 nm were synthesized and characterized at a concentration of 5,36×1010 pcs/ml with an absorption maximum of 840 nm. Gold nanostars were coated with thiolated polyethylene glycol, its amount was about 8×104 molecules per 1 particle and about 4,4×1015 molecules/ml in the colloid. The zeta potential of gold nanostars coated with PEG-SH was −2.3 mV. The combined effect of gold nanostars and low-intensity infrared (808 nm) laser radiation on the bacteria Staphylococcus aureus 209 P and Escherichia coli 113-13 has been studied. Incubation of suspensions of microorganisms in the presence of nanoparticles without access to light did not lead to a significant reduction in the number of bacteria. Irradiation for 30 min of bacterial suspensions containing nanoparticles caused the death of 39% of the S. aureus population and 80% of the E. coli population. During the thermometry of the studied suspensions, it was found that the temperature increase is dose-dependent. The increase in temperature of the control samples that did not contain photothermal agents did not exceed 1 °C in both cases throughout the entire time of the experiment. For suspensions of bacteria (equally S. aureus and E. coli) incubated with gold nanostars during irradiation, an increase in temperature indicators, on average, by 4 °C was revealed. The more pronounced antibacterial activity of the combination of gold nanostars and infrared (808 nm) laser radiation against E. coli can also be explained by the greater sensitivity of gram-negative microorganisms to photothermal exposure.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81210567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-21DOI: 10.18500/1816-9775-2023-23-1-39-50
E. G. Kulapina, Milena N. Gasparyan, O. I. Kulapina, V. D. Ankina
Doxycycline has a wide range of antimicrobial and anti-inflammatory properties and is used to treat various infectious diseases. Spectroscopic, chromatographic, electrochemical, immunoassay and other methods are used to determine tetracycline antibiotics in various objects. Planar sensors allow express detection of antibiotics in small sample volumes. Planar potentiometric sensors based on ion associations doxycycline – tetraphenylborate (Copt = 2–3%), modifier – polyaniline, for the quantitative determination of doxycycline have been developed. It has been found that for non-modificated sensors, the linearity intervals of electrode functions are 1×10 -4 – 5×10 -3 M, Cmin – 5,0×10-5 M, angular coefficients – 50±2 mV/pC, response time – 25 s. It is shown that the introduction of a modifier into the carbon-containing ink of sensors leads to the stabilization of their potential, to an increase in the angular coefficients of electrode functions (56±1), reduced response time (20 s) and reduced detection limit (4.0 ×10-5 M). It is shown that the introduction of the modifier into the carbon-containing ink of the sensors leads to stabilization of their potential, to an increase in the angular coefficients of electrode functions (56±1), a decrease in response time (20 seconds) and a decrease in the detection limit (4.0 ×10-5 M). Doxycycline sensitive sensors have selectivity to the base ion (Ki/j < < 1) and inorganic cations (Ki/j (K+) = 0.61; Ki/j (Na+) = 0,01; Ki/j (Mg 2+) = 0,01; Ki/j (Ca2+) = 0.09), and indicate the possibility of determining doxycycline in human biological fluids. Against the background of oral liquid, the angular coefficients of electrode functions (45±1 mV/pS) decrease, which is associated with the background influence of oral liquid. The developed planar sensors are used to determination doxycycline in medicinal and biological media.
{"title":"Electroanalytic properties of unmodified and polyaniline-modified planar sensors sensitive to doxycycline","authors":"E. G. Kulapina, Milena N. Gasparyan, O. I. Kulapina, V. D. Ankina","doi":"10.18500/1816-9775-2023-23-1-39-50","DOIUrl":"https://doi.org/10.18500/1816-9775-2023-23-1-39-50","url":null,"abstract":"Doxycycline has a wide range of antimicrobial and anti-inflammatory properties and is used to treat various infectious diseases. Spectroscopic, chromatographic, electrochemical, immunoassay and other methods are used to determine tetracycline antibiotics in various objects. Planar sensors allow express detection of antibiotics in small sample volumes. Planar potentiometric sensors based on ion associations doxycycline – tetraphenylborate (Copt = 2–3%), modifier – polyaniline, for the quantitative determination of doxycycline have been developed. It has been found that for non-modificated sensors, the linearity intervals of electrode functions are 1×10 -4 – 5×10 -3 M, Cmin – 5,0×10-5 M, angular coefficients – 50±2 mV/pC, response time – 25 s. It is shown that the introduction of a modifier into the carbon-containing ink of sensors leads to the stabilization of their potential, to an increase in the angular coefficients of electrode functions (56±1), reduced response time (20 s) and reduced detection limit (4.0 ×10-5 M). It is shown that the introduction of the modifier into the carbon-containing ink of the sensors leads to stabilization of their potential, to an increase in the angular coefficients of electrode functions (56±1), a decrease in response time (20 seconds) and a decrease in the detection limit (4.0 ×10-5 M). Doxycycline sensitive sensors have selectivity to the base ion (Ki/j < < 1) and inorganic cations (Ki/j (K+) = 0.61; Ki/j (Na+) = 0,01; Ki/j (Mg 2+) = 0,01; Ki/j (Ca2+) = 0.09), and indicate the possibility of determining doxycycline in human biological fluids. Against the background of oral liquid, the angular coefficients of electrode functions (45±1 mV/pS) decrease, which is associated with the background influence of oral liquid. The developed planar sensors are used to determination doxycycline in medicinal and biological media.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"1995 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75232797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-21DOI: 10.18500/1816-9775-2023-23-1-110-123
I. V. Shilova, A. Kashin, A. S. Parkhomenko, A. V. Bogoslov, T. Kritskaya, Michail Yu. Kasatkin
The article presents a phytocoenotic description of 23 plant communities with Colchicum bulbocodium subsp. versicolor studied during the period of mass flowering in 2014–2018. It was found that, across the Lower Volga region, the studied communities with C. bulbocodium subsp. versicolor are mostly confined to the slopes of south- and east-facing arroyos and more seldom to the southern and northern hill slopes, plains, arroyo and liman bases, and floodmeadows. During the period of mass flowering, 207 vascular plants were detected in the studied communities. Every community description included 9 to 36 species. Biological diversity was assessed with the Shannon index and polydominance index; the degree of dominance was measured with the Simpson index. The species similarity of the communities was evaluated through pairwise comparison with the Jaccard coefficient. It was revealed that C. bulbocodium subsp. versicolor occurs in communities varying in diversity and species composition. The subspecies is not confined to specific phytocoenoses. It usually grows on rich and, more seldom, fairly rich and slightly saline soils. Their alluviality is more often weak rather than moderate. Watering usually corresponds to the dry steppe or semi-desert climate type, rarely to the middle steppe type, being moderately variable and in some cases highly variable. The impact of grazing is usually weak, but it is either moderate or strong in some communities. The communities with C. bulbocodium subsp. versicolor are dominated by hemicryptophytes: mostly tap-root, short-rhizome and long-rhizome herbaceous perennials. In phytocoenotic terms, most species belong to the zonal type of vegetation, namely steppe vegetation. The participation of meadow species is prominent. The share of weed species is rather high, which indicates a significant anthropogenic load on the studied communities.
{"title":"The ecological and coenotic features of plant communities containing Colchicum bulbocodium subsp. versicolor (Colchicaceae) in the Lower Volga region","authors":"I. V. Shilova, A. Kashin, A. S. Parkhomenko, A. V. Bogoslov, T. Kritskaya, Michail Yu. Kasatkin","doi":"10.18500/1816-9775-2023-23-1-110-123","DOIUrl":"https://doi.org/10.18500/1816-9775-2023-23-1-110-123","url":null,"abstract":"The article presents a phytocoenotic description of 23 plant communities with Colchicum bulbocodium subsp. versicolor studied during the period of mass flowering in 2014–2018. It was found that, across the Lower Volga region, the studied communities with C. bulbocodium subsp. versicolor are mostly confined to the slopes of south- and east-facing arroyos and more seldom to the southern and northern hill slopes, plains, arroyo and liman bases, and floodmeadows. During the period of mass flowering, 207 vascular plants were detected in the studied communities. Every community description included 9 to 36 species. Biological diversity was assessed with the Shannon index and polydominance index; the degree of dominance was measured with the Simpson index. The species similarity of the communities was evaluated through pairwise comparison with the Jaccard coefficient. It was revealed that C. bulbocodium subsp. versicolor occurs in communities varying in diversity and species composition. The subspecies is not confined to specific phytocoenoses. It usually grows on rich and, more seldom, fairly rich and slightly saline soils. Their alluviality is more often weak rather than moderate. Watering usually corresponds to the dry steppe or semi-desert climate type, rarely to the middle steppe type, being moderately variable and in some cases highly variable. The impact of grazing is usually weak, but it is either moderate or strong in some communities. The communities with C. bulbocodium subsp. versicolor are dominated by hemicryptophytes: mostly tap-root, short-rhizome and long-rhizome herbaceous perennials. In phytocoenotic terms, most species belong to the zonal type of vegetation, namely steppe vegetation. The participation of meadow species is prominent. The share of weed species is rather high, which indicates a significant anthropogenic load on the studied communities.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74637186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-21DOI: 10.18500/1816-9775-2023-23-1-77-85
N. Dyakova
Voronezh region is one of the most important areas of agriculture and crop production. The purpose of the study was to study the patterns of accumulation of the most dangerous heavy metals in wild medicinal plant raw materials of various ecotopes of the Voronezh region. The accumulation of heavy metals (lead, mercury, cadmium, nickel, copper, zinc, cobalt, chromium) and arsenic was studied, using an example of 10 types of medicinal plant raw materials, which were produced in 51 ecotopes of the Voronezh region. When analyzing the average values of the accumulation coefficients of heavy metals and arsenic by all studied types of medicinal plant raw materials, it is possible to build up a series of decreases in the total average accumulation efficiency of the determined elements for these plants, which is as follows: zinc> copper > cadmium > cobalt > nickel > arsenic > chromium > lead > mercury. When analyzing the values of accumulation coefficients, rows of decreasing efficiency of accumulation of elements in all types of medicinal plant raw materials studied are constructed. A detailed analysis of the characteristics of the accumulation of heavy metals and arsenic from soils in medicinal plant raw materials showed the presence of aclose positive relationship between concentrations in soil and plants for most of the determined elements, which indicates the predominant contamination of plant raw materials through the soil.
{"title":"Study of patterns of accumulation of the most dangerous ecotoxicants in wild medicinal plant raw materials of various ecotopes of the Voronezh region","authors":"N. Dyakova","doi":"10.18500/1816-9775-2023-23-1-77-85","DOIUrl":"https://doi.org/10.18500/1816-9775-2023-23-1-77-85","url":null,"abstract":"Voronezh region is one of the most important areas of agriculture and crop production. The purpose of the study was to study the patterns of accumulation of the most dangerous heavy metals in wild medicinal plant raw materials of various ecotopes of the Voronezh region. The accumulation of heavy metals (lead, mercury, cadmium, nickel, copper, zinc, cobalt, chromium) and arsenic was studied, using an example of 10 types of medicinal plant raw materials, which were produced in 51 ecotopes of the Voronezh region. When analyzing the average values of the accumulation coefficients of heavy metals and arsenic by all studied types of medicinal plant raw materials, it is possible to build up a series of decreases in the total average accumulation efficiency of the determined elements for these plants, which is as follows: zinc> copper > cadmium > cobalt > nickel > arsenic > chromium > lead > mercury. When analyzing the values of accumulation coefficients, rows of decreasing efficiency of accumulation of elements in all types of medicinal plant raw materials studied are constructed. A detailed analysis of the characteristics of the accumulation of heavy metals and arsenic from soils in medicinal plant raw materials showed the presence of aclose positive relationship between concentrations in soil and plants for most of the determined elements, which indicates the predominant contamination of plant raw materials through the soil.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78524143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.18500/1816-9775-2022-22-4-409-418
E. Pleshakova, Milena A. Kasatkina, C. Ngun, M. Reshetnikov
From the stand point of the possible use of microorganisms in water purification biotechnologies, studying microorganisms capable of removing dangerous pollutants such as heavy metals from the aquatic environment is of great importance. Microbial screening was conducted on isolates from microbiocenoses of highly magnetic soils of the city Mednogorsk (Orenburg region, Russia), to assess their resistance to manganese (II) ions. The objects of this research were nine strains of manganese-oxidizing microorganisms and two strains of iron-oxidizing microorganisms: Bacillus megaterium 69.3 and B. megaterium 69.5. It was observed that, most of the studied microorganisms were characterized by increased resistance to manganese (II) ions when grown on an agar medium. Maximum resistance to Mn (II) was observed in microbial strains: 55.2 and B. megaterium 69.5. The maximum tolerant concentration (MTC) of Mn (II) for these microorganisms was 300 and 350 mmol/L; the minimum inhibitory concentration (MIC) was 350 and 450 mmol/L, respectively. When studying the growth of the two microbial strains: 55.2 and B. megaterium 69.5 in liquid media for 5 days. It was discovered that, when cultured in a selective medium, microbial resistance of the strains to Mn (II) was higher compared to when cultured in a nutrient LB medium. The results showed high resistance of microorganisms to a range of Mn (II) concentrations: from 0,5 to 250 mmol/L in a liquid medium. Maximum growth of the studied microbial strains was observed at Mn (II) concentration of 10 mmol/L. These microbial strains with high resistance to Mn (II) open up the prospect of their practical use for biotechnological purposes involving the purification of drinking and wastewater from high manganese content.
{"title":"A study of manganese-oxidizing microorganisms isolated from microbiocenoses of highly magnetic soils","authors":"E. Pleshakova, Milena A. Kasatkina, C. Ngun, M. Reshetnikov","doi":"10.18500/1816-9775-2022-22-4-409-418","DOIUrl":"https://doi.org/10.18500/1816-9775-2022-22-4-409-418","url":null,"abstract":"From the stand point of the possible use of microorganisms in water purification biotechnologies, studying microorganisms capable of removing dangerous pollutants such as heavy metals from the aquatic environment is of great importance. Microbial screening was conducted on isolates from microbiocenoses of highly magnetic soils of the city Mednogorsk (Orenburg region, Russia), to assess their resistance to manganese (II) ions. The objects of this research were nine strains of manganese-oxidizing microorganisms and two strains of iron-oxidizing microorganisms: Bacillus megaterium 69.3 and B. megaterium 69.5. It was observed that, most of the studied microorganisms were characterized by increased resistance to manganese (II) ions when grown on an agar medium. Maximum resistance to Mn (II) was observed in microbial strains: 55.2 and B. megaterium 69.5. The maximum tolerant concentration (MTC) of Mn (II) for these microorganisms was 300 and 350 mmol/L; the minimum inhibitory concentration (MIC) was 350 and 450 mmol/L, respectively. When studying the growth of the two microbial strains: 55.2 and B. megaterium 69.5 in liquid media for 5 days. It was discovered that, when cultured in a selective medium, microbial resistance of the strains to Mn (II) was higher compared to when cultured in a nutrient LB medium. The results showed high resistance of microorganisms to a range of Mn (II) concentrations: from 0,5 to 250 mmol/L in a liquid medium. Maximum growth of the studied microbial strains was observed at Mn (II) concentration of 10 mmol/L. These microbial strains with high resistance to Mn (II) open up the prospect of their practical use for biotechnological purposes involving the purification of drinking and wastewater from high manganese content.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87653705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.18500/1816-9775-2022-22-4-364-372
Angelina A. Kutina, E. G. Sumina, N. Yurasov, V. Z. Uglanova
The chromatographic behavior of atorvastatin, rosuvastatin and simvastatin in the presence of the main modifiers of the mobile phase: elution strength and buffer solution, has been studied by means of the reverse-phase high-performance liquid chromatography (HPLC) method. Optimal modification conditions have been found and their application for the determination of statins under study in the pharmaceutical preparations Atorvastatin-OBL, Liprimar and Tulip has been shown. The Sr value is 0.03. The relative determination error does not exceed 7%.
{"title":"Determination of statins by reverse-phase HPLC in mobile phases with different modification options","authors":"Angelina A. Kutina, E. G. Sumina, N. Yurasov, V. Z. Uglanova","doi":"10.18500/1816-9775-2022-22-4-364-372","DOIUrl":"https://doi.org/10.18500/1816-9775-2022-22-4-364-372","url":null,"abstract":"The chromatographic behavior of atorvastatin, rosuvastatin and simvastatin in the presence of the main modifiers of the mobile phase: elution strength and buffer solution, has been studied by means of the reverse-phase high-performance liquid chromatography (HPLC) method. Optimal modification conditions have been found and their application for the determination of statins under study in the pharmaceutical preparations Atorvastatin-OBL, Liprimar and Tulip has been shown. The Sr value is 0.03. The relative determination error does not exceed 7%.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89041822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.18500/1816-9775-2022-22-4-460-473
A. V. Bogoslov, A. S. Parkhomenko, A. O. Kondratieva, I. V. Shilova, Anastasiya D. Orlowa, A. Kashin
Data on morphological variability of 13 populations of the species Colchicum laetum Steven, rare for the Russian Federation, in the territory of the Volgograd region, the Republic of Kalmykia and the Stavropol Territory are presented. A strong morphological variability of populations on the territory of the regions was noted both at the intra- and interpopulation level. Apparently, the heterogeneity of the environment in space, both significant and local, is well felt by plants of populations that adapt to this heterogeneity in different ways. However, the revealed change in the morphological parameters of C. laetum along the gradient of geographic coordinates indicates that, above all, weather and climatic factors determine the variability of morphological parameters in this species.
{"title":"Morphological variability of Colchicum laetum Steven (Colchidaceae) populations in the Lower Volga region and surrounding territories","authors":"A. V. Bogoslov, A. S. Parkhomenko, A. O. Kondratieva, I. V. Shilova, Anastasiya D. Orlowa, A. Kashin","doi":"10.18500/1816-9775-2022-22-4-460-473","DOIUrl":"https://doi.org/10.18500/1816-9775-2022-22-4-460-473","url":null,"abstract":"Data on morphological variability of 13 populations of the species Colchicum laetum Steven, rare for the Russian Federation, in the territory of the Volgograd region, the Republic of Kalmykia and the Stavropol Territory are presented. A strong morphological variability of populations on the territory of the regions was noted both at the intra- and interpopulation level. Apparently, the heterogeneity of the environment in space, both significant and local, is well felt by plants of populations that adapt to this heterogeneity in different ways. However, the revealed change in the morphological parameters of C. laetum along the gradient of geographic coordinates indicates that, above all, weather and climatic factors determine the variability of morphological parameters in this species.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75257148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.18500/1816-9775-2022-22-4-445-454
Yu.V. Krasova, V. Fadeev, Y. Moiseeva, Y. Gusev, M. Chumakov
We optimizes the composition and concentration of the enzymes, the time for enzymatic treatment and the volume of the enzyme mixture. We also optimized the concentration osmotic of agent, the centrifugation mode, and filter pore size for protoplasts isolating from epidermal cells of maize roots (Zea mays L.) of the Brown Marker (BM) line. It was found that 150 minutes is the optimal time for 150 mg root tissue maceration. The yield of intact protoplasts was ~ 4.4 ± 0.2 × 105 cells/mL at the following concentrations of enzymes and osmotic stabilizer: cellulase – 17,4, pectolase – 1.2, hemicellulase – 0.07, D-mannitol – 9.3%. The the concentration of protoplasts was to 23 times higher (p < 0.05) in 800 μl, compared with 200 μl of the enzyme mixture with equal concentrations of enzymes and osmotic stabilizer. It was found that filtration of 800 μl protoplast suspension through a filter with a pore size of 15 × 15 microns increases the yield of protoplasts up to 3.3 times, compared with a filter with a pore size of 15×39. Fractional centrifugation without preliminary filtration of the solution and the flotation method did not produce an increase in the yield of protoplasts. The residual number and protoplast wholeness after ~ 20 hours at +3 °C incubation was evaluated. The protoplast number decreased up to 2 times (p < 0.05) after electroporation.
{"title":"Optimization of the technique for maize protoplast isolation and their nativity after electroporation","authors":"Yu.V. Krasova, V. Fadeev, Y. Moiseeva, Y. Gusev, M. Chumakov","doi":"10.18500/1816-9775-2022-22-4-445-454","DOIUrl":"https://doi.org/10.18500/1816-9775-2022-22-4-445-454","url":null,"abstract":"We optimizes the composition and concentration of the enzymes, the time for enzymatic treatment and the volume of the enzyme mixture. We also optimized the concentration osmotic of agent, the centrifugation mode, and filter pore size for protoplasts isolating from epidermal cells of maize roots (Zea mays L.) of the Brown Marker (BM) line. It was found that 150 minutes is the optimal time for 150 mg root tissue maceration. The yield of intact protoplasts was ~ 4.4 ± 0.2 × 105 cells/mL at the following concentrations of enzymes and osmotic stabilizer: cellulase – 17,4, pectolase – 1.2, hemicellulase – 0.07, D-mannitol – 9.3%. The the concentration of protoplasts was to 23 times higher (p < 0.05) in 800 μl, compared with 200 μl of the enzyme mixture with equal concentrations of enzymes and osmotic stabilizer. It was found that filtration of 800 μl protoplast suspension through a filter with a pore size of 15 × 15 microns increases the yield of protoplasts up to 3.3 times, compared with a filter with a pore size of 15×39. Fractional centrifugation without preliminary filtration of the solution and the flotation method did not produce an increase in the yield of protoplasts. The residual number and protoplast wholeness after ~ 20 hours at +3 °C incubation was evaluated. The protoplast number decreased up to 2 times (p < 0.05) after electroporation.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83291721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.18500/1816-9775-2022-22-4-419-426
L. Sedelnikova, O. Tsandekova
The article for the first time presents the comparative results of the content of ash substances, sulfur, phenolic compounds (tannins), ascorbic acid in the leaves of plants Iris hybrida, Hosta decorata, H. albomarginata, H. lancifolia, cultivated in the Central Siberian Botanical Garden (CSBS, Akademgorodok) and used in landscaping the urban environment of the Berdsk and Koltsovo settlements (Novosibirsk region) during the seasonal development in 2020. The specificity of distribution of sulfur, ash, tannins, ascorbic acid in leaves of the studied species was revealed. The quantitative content of the presented groups of substances in the leaves during the flowering period of these objects of study has been determined. Under the conditions of urban environment, an increase in the content of ash substances in the leaves of I. hybrida by 2,5–3,6 times and H. albomarginata, H. lancifolia, and H. decorata by 1,4–1,7 times was found. The general tendency of the accumulation of sulfur and ash in leaves of the species of the genus Hosta, with the greatest value in H. albomarginata growing in green areas of Berdsk was noted. Under the same conditions, the concentration of sulfur in the leaves of I. hybrida increased 1,3 fold compared with Hosta decorata. The content of ascorbic acid and tannins in the leaves of H. albomarginata, H. lancifolia, H. decorata, I. hybrida is 1,1–2,4 times lower in the urban environment of Berdsk and Koltsovo than in plants grown in conditions with a favorable environmental situation (CSBS). In terms of biochemical, habitual, and rhythmological evaluation of the indicators, the species present a comparative series in descending values: H. lancifolia > H. albomarginata > H. decorata > I. hybrida.
{"title":"Assessment of the content of biologically active substances and chemical elements in the leaves of Hosta and Iris hybrida in the landscaping of the Novosibirsk region","authors":"L. Sedelnikova, O. Tsandekova","doi":"10.18500/1816-9775-2022-22-4-419-426","DOIUrl":"https://doi.org/10.18500/1816-9775-2022-22-4-419-426","url":null,"abstract":"The article for the first time presents the comparative results of the content of ash substances, sulfur, phenolic compounds (tannins), ascorbic acid in the leaves of plants Iris hybrida, Hosta decorata, H. albomarginata, H. lancifolia, cultivated in the Central Siberian Botanical Garden (CSBS, Akademgorodok) and used in landscaping the urban environment of the Berdsk and Koltsovo settlements (Novosibirsk region) during the seasonal development in 2020. The specificity of distribution of sulfur, ash, tannins, ascorbic acid in leaves of the studied species was revealed. The quantitative content of the presented groups of substances in the leaves during the flowering period of these objects of study has been determined. Under the conditions of urban environment, an increase in the content of ash substances in the leaves of I. hybrida by 2,5–3,6 times and H. albomarginata, H. lancifolia, and H. decorata by 1,4–1,7 times was found. The general tendency of the accumulation of sulfur and ash in leaves of the species of the genus Hosta, with the greatest value in H. albomarginata growing in green areas of Berdsk was noted. Under the same conditions, the concentration of sulfur in the leaves of I. hybrida increased 1,3 fold compared with Hosta decorata. The content of ascorbic acid and tannins in the leaves of H. albomarginata, H. lancifolia, H. decorata, I. hybrida is 1,1–2,4 times lower in the urban environment of Berdsk and Koltsovo than in plants grown in conditions with a favorable environmental situation (CSBS). In terms of biochemical, habitual, and rhythmological evaluation of the indicators, the species present a comparative series in descending values: H. lancifolia > H. albomarginata > H. decorata > I. hybrida.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84868509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-15DOI: 10.18500/1816-9775-2022-22-4-455-459
V. Vasjukov, M. Nikelshparg
As a result of the revision of herbarium samples of Thymus (Lamiaceae) collected in the central part of the Russian Caucasus (Kabardino-Balkarian Republic, Karachay-Cherkess Republic, Republic of North Ossetia-Alania, Stavropol Territory) and transferred to the Herbarium of the Institute of Ecology of the Volga river basin of the Russian Academy of Sciences (PVB), 17 species of the genus Thymus were identified: T. buschianus Klokov et Des.-Shost., T. caucasicus Willd. ex Ronniger, T. daghestanicus Klokov et Des.-Shost., T. elisabethae Klokov et Des.-Shost., T. latissimus Klokov, T. lipskyi Klokov et Des.-Shost., T. mashukensis Klokov, T. menitskyi Vasjukov, T. nummularius M. Bieb., T. osseticus Vasjukov, T. pastoralis Iljin ex Klokov, T. pseudonummularius Klokov et Des.-Shost., T. pseudopulegioides Klokov et Des.-Shost., T. rariflorus K. Koch, T. teberdensis Klokov, T. terekensis Klokov, T. transcaucasicus Ronniger.
{"title":"Samples of Thymus (Lamiaceae) from the central part of the Russian Caucasus, stored in the Herbarium of the Institute of Ecology of the Volga river basin of the Russian Academy of Science (PVB)","authors":"V. Vasjukov, M. Nikelshparg","doi":"10.18500/1816-9775-2022-22-4-455-459","DOIUrl":"https://doi.org/10.18500/1816-9775-2022-22-4-455-459","url":null,"abstract":"As a result of the revision of herbarium samples of Thymus (Lamiaceae) collected in the central part of the Russian Caucasus (Kabardino-Balkarian Republic, Karachay-Cherkess Republic, Republic of North Ossetia-Alania, Stavropol Territory) and transferred to the Herbarium of the Institute of Ecology of the Volga river basin of the Russian Academy of Sciences (PVB), 17 species of the genus Thymus were identified: T. buschianus Klokov et Des.-Shost., T. caucasicus Willd. ex Ronniger, T. daghestanicus Klokov et Des.-Shost., T. elisabethae Klokov et Des.-Shost., T. latissimus Klokov, T. lipskyi Klokov et Des.-Shost., T. mashukensis Klokov, T. menitskyi Vasjukov, T. nummularius M. Bieb., T. osseticus Vasjukov, T. pastoralis Iljin ex Klokov, T. pseudonummularius Klokov et Des.-Shost., T. pseudopulegioides Klokov et Des.-Shost., T. rariflorus K. Koch, T. teberdensis Klokov, T. terekensis Klokov, T. transcaucasicus Ronniger.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74091076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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