Pub Date : 2021-12-16DOI: 10.18500/1816-9775-2021-21-4-368-377
S. Salmenbayev, Nazgul K. Nurgaysinova, G. M. Yessilkanov, Аray E. Temirzhanova, S. Makarychev
The relevance of the research is caused by the need to develop a methodological base for determining 151Sm content in the soil cover of radioactively contaminated territories of Kazakhstan. The developed method for the determining of 151Sm will make it possible to assess the levels of soil contamination with this radionuclide, to determine the character of its spatial distribution, to allow estimating the internal exposure doses for the personnel and the population. The aim of the research is to carry out the isolation and radiochemical purification of samarium isotopes from acid solutions via using ion-exchange resins AV 17×8 and KU-2. Objects: salt solutions based on nitric and hydrochloric acid containing the stable isotopes of some natural, artificial β-emitters and isotopes of U and Th. The concentrations of nitric and hydrochloric acids were equal to the concentrations of the same acids used in the routine analysis of Pu and Am. Concentrations of chemical elements were determined using the Agilent 7700x quadrupole mass spectrometer and the iCAP 6300 Duo atomic emission spectrometer. The results of the experiments on the isolation and radiochemical purification of samarium isotopes from acidic solutions using anion-exchange resin AV 17×8 and cation-exchange resin KU-2 have been presented. It has been shown that the Sm-fraction can be purified from alkaline elements, Tl and U isotopes using the KU-2 cation-exchange resin. In turn, the isotopes U, Fe and Co can be removed using an anion exchange resin in 9M HCl media.
{"title":"Isolation and radiochemical purification of samarium isotopes using ion exchange resins АВ 17×8 AND KU-2","authors":"S. Salmenbayev, Nazgul K. Nurgaysinova, G. M. Yessilkanov, Аray E. Temirzhanova, S. Makarychev","doi":"10.18500/1816-9775-2021-21-4-368-377","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-4-368-377","url":null,"abstract":"The relevance of the research is caused by the need to develop a methodological base for determining 151Sm content in the soil cover of radioactively contaminated territories of Kazakhstan. The developed method for the determining of 151Sm will make it possible to assess the levels of soil contamination with this radionuclide, to determine the character of its spatial distribution, to allow estimating the internal exposure doses for the personnel and the population. The aim of the research is to carry out the isolation and radiochemical purification of samarium isotopes from acid solutions via using ion-exchange resins AV 17×8 and KU-2. Objects: salt solutions based on nitric and hydrochloric acid containing the stable isotopes of some natural, artificial β-emitters and isotopes of U and Th. The concentrations of nitric and hydrochloric acids were equal to the concentrations of the same acids used in the routine analysis of Pu and Am. Concentrations of chemical elements were determined using the Agilent 7700x quadrupole mass spectrometer and the iCAP 6300 Duo atomic emission spectrometer. The results of the experiments on the isolation and radiochemical purification of samarium isotopes from acidic solutions using anion-exchange resin AV 17×8 and cation-exchange resin KU-2 have been presented. It has been shown that the Sm-fraction can be purified from alkaline elements, Tl and U isotopes using the KU-2 cation-exchange resin. In turn, the isotopes U, Fe and Co can be removed using an anion exchange resin in 9M HCl media.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84500104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-353-363
O. I. Yudakova
The Biological Faculty of the Saratov State University turns 90 years old in 2021. The first students were enrolled at the Faculty in the 1931–1932 academic year. However, the faculty’s history began long before its opening, when the Department of Botany and the Department of Zoology with Comparative Anatomy were organized in the new Saratov Imperial Nikolaev University in 1909. The article describes important events in the life of the Faculty and the contribution of scientists who stood at the origins of the birth of biological science at Saratov State University and played an important role in the formation and development of the Biological Faculty. A whole galaxy of famous biologists and eminent personalities worked at the university at different times. These are the academicians N. I. Vavilov, N. A. Maksimov, A. A. Richter, corresponding members A. Ya. Gordyagin, S. D. Lvov, professors D. E. Yanishevsky, A. D. Fursaev, I. V. Krasovskaya, V. S. Elpatevskiy, B. K. Fenyuk, P. A. Vunder, A. A. Chiguryaeva, S. S. Khokhlov, M. P. Gnutenko, V. V. Ignatov and others. They founded scientific schools, laid down faculty traditions and set a high standard for biological education, which the modern faculty team strives to maintain to this day.
{"title":"90 years of the Biology Faculty of the Saratov State University","authors":"O. I. Yudakova","doi":"10.18500/1816-9775-2021-21-3-353-363","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-353-363","url":null,"abstract":"The Biological Faculty of the Saratov State University turns 90 years old in 2021. The first students were enrolled at the Faculty in the 1931–1932 academic year. However, the faculty’s history began long before its opening, when the Department of Botany and the Department of Zoology with Comparative Anatomy were organized in the new Saratov Imperial Nikolaev University in 1909. The article describes important events in the life of the Faculty and the contribution of scientists who stood at the origins of the birth of biological science at Saratov State University and played an important role in the formation and development of the Biological Faculty. A whole galaxy of famous biologists and eminent personalities worked at the university at different times. These are the academicians N. I. Vavilov, N. A. Maksimov, A. A. Richter, corresponding members A. Ya. Gordyagin, S. D. Lvov, professors D. E. Yanishevsky, A. D. Fursaev, I. V. Krasovskaya, V. S. Elpatevskiy, B. K. Fenyuk, P. A. Vunder, A. A. Chiguryaeva, S. S. Khokhlov, M. P. Gnutenko, V. V. Ignatov and others. They founded scientific schools, laid down faculty traditions and set a high standard for biological education, which the modern faculty team strives to maintain to this day.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78155546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-260-266
A. Tumskaia, I. V. Kosyreva
Test means based on immobilized Fehling’s reagent for the determination of cephalexin have been obtained. Methods for visual and colorimetric assessment of antibiotic concentration using a smartphone camera have been developed. The optimal conditions for the indicator reaction have been selected with varying heating time and temperature. For a visual semi-quantitative assessment of the content of cephalexin, a color scale has been obtained. The metrological characteristics of the test method have been determined: the range of the determined contents is 0.5–16 mg/ml, the unreliability interval is 0.1–0.4 mg/ml, and the detection limit is 0.4 mg / ml. For the colorimetric assessment of the concentration of cephalexin, a linear dependence of the intensity of the Blue channel on the logarithm of the concentration of cephalexin (у = –96x + 144, r 2 = 0.99) has been constructed, and linear dependences of the area (у = –36986x + 62458, r 2 = 0.96) and perimeter (у = –270x + 786, r 2 = 0.93) from the logarithm of the concentration of cephalexin have been obtained. The range of the determined contents was 0.1–16 mg/ml, the lower limit was 0.1 mg / ml. The verification of the correctness of the developed test methods was carried out by the «introduced-found» method (Sr 0.13).
{"title":"Express determination of cephalexin","authors":"A. Tumskaia, I. V. Kosyreva","doi":"10.18500/1816-9775-2021-21-3-260-266","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-260-266","url":null,"abstract":"Test means based on immobilized Fehling’s reagent for the determination of cephalexin have been obtained. Methods for visual and colorimetric assessment of antibiotic concentration using a smartphone camera have been developed. The optimal conditions for the indicator reaction have been selected with varying heating time and temperature. For a visual semi-quantitative assessment of the content of cephalexin, a color scale has been obtained. The metrological characteristics of the test method have been determined: the range of the determined contents is 0.5–16 mg/ml, the unreliability interval is 0.1–0.4 mg/ml, and the detection limit is 0.4 mg / ml. For the colorimetric assessment of the concentration of cephalexin, a linear dependence of the intensity of the Blue channel on the logarithm of the concentration of cephalexin (у = –96x + 144, r 2 = 0.99) has been constructed, and linear dependences of the area (у = –36986x + 62458, r 2 = 0.96) and perimeter (у = –270x + 786, r 2 = 0.93) from the logarithm of the concentration of cephalexin have been obtained. The range of the determined contents was 0.1–16 mg/ml, the lower limit was 0.1 mg / ml. The verification of the correctness of the developed test methods was carried out by the «introduced-found» method (Sr 0.13).","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82784300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-347-352
V. A. Shushunov, I. V. Shilova, A. S. Parhomenko, A. Kashin
During the flowering period of Colchicum laetum Stephen (September 16–19, 2020), seven habitats were studied in the Volgograd region. C. laetum occurs on small (0.006–4 ha) unploughed areas of the steppes adjacent to gullies. In the community, 28 species of vascular plants were recorded, including C. laetum, only 10 species vegetated (from 2 to 7 in the community): Artemisia austriaca Jacq., A. santonicum L., Bassia prostrata (L.) Beck, Cynanchum acutum L., Dianthus polymorphus M. Bieb., Euphorbia seguieriana Neck., E. virgata Waldst., Grubovia sedoides (Pall.) GL Chu, Limonium bungei (Claus) Gamajun., Medicago falcata L. The participation of C. laetum is from single individuals to quite abundant, with a projective cover not exceeding 5%. A preliminary assessment of habitat by vegetation according to the Ramenskoye scales showed that one community is quite rich, one is rich and one is not rich. The humidification is from dry steppe to desert. Only one community does not experience anthropogenic impact. In the second the arrival of vehicles was marked. In the third grazing is carried out, accompanied by a failure.
{"title":"Ecological and cenotic characteristics of Colchicum laetum (Colchicaceae) habitats in the Volgograd region","authors":"V. A. Shushunov, I. V. Shilova, A. S. Parhomenko, A. Kashin","doi":"10.18500/1816-9775-2021-21-3-347-352","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-347-352","url":null,"abstract":"During the flowering period of Colchicum laetum Stephen (September 16–19, 2020), seven habitats were studied in the Volgograd region. C. laetum occurs on small (0.006–4 ha) unploughed areas of the steppes adjacent to gullies. In the community, 28 species of vascular plants were recorded, including C. laetum, only 10 species vegetated (from 2 to 7 in the community): Artemisia austriaca Jacq., A. santonicum L., Bassia prostrata (L.) Beck, Cynanchum acutum L., Dianthus polymorphus M. Bieb., Euphorbia seguieriana Neck., E. virgata Waldst., Grubovia sedoides (Pall.) GL Chu, Limonium bungei (Claus) Gamajun., Medicago falcata L. The participation of C. laetum is from single individuals to quite abundant, with a projective cover not exceeding 5%. A preliminary assessment of habitat by vegetation according to the Ramenskoye scales showed that one community is quite rich, one is rich and one is not rich. The humidification is from dry steppe to desert. Only one community does not experience anthropogenic impact. In the second the arrival of vehicles was marked. In the third grazing is carried out, accompanied by a failure.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79052024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-267-273
Elmira I. Tsygulyova, S. Doronin
For the efficient preconcentration of azo compounds – products of the interaction of 4-nitrophenyldiazonium with thymol, a system 4-nitroaniline (4-NA) – NO2- – Triton X-100 – NaOH – ethanol has been proposed. The optimal conditions for the formation of micellarsaturated phases of the system under study have been established: 3.10-4 M 4-NA – 3.10-4 M NO2- – 5% Triton X-100 – 2.8 M NaOH – 10 vol. % С 2Н5ОН. A spectrophotometric study of the above system has been carried out. A linear dependence was built in the coordinates A (at λ max = 552 nm) vs с(thymol), which is described by an equation of the form A = f(c), A = 26291c + 0.02; R2 = 0.997. The range of the determined contents of thymol is (2·10-6 – 4·10-5) mol/l. A technique for the colorimetric determination of thymol in aqueous media (color channel G) has been developed. The intensity of the channel G chromaticity (IG) is linearly dependent on pc(thymol) in accordance with the equation IG = 54.2pc – 267, R 2 = 0; the lower limit of the determined contents of thymol is 1.10-6 mol/l, which is two times less than in the variant of its spectrophotometric determination. The profiles of petal diagrams in the color coordinates of the RGB CMYK model have been constructed; the dependences of their area (S) and perimeter (P) on the thymol concentration have been obtained (P: y = 278x – 10.13; R 2 = 0.97; S: y = 20182x – 87649, R 2 = 0.99).
为了高效富集4-硝基苯基重氮鎓与百里酚相互作用的偶氮化合物，提出了4-硝基苯胺(4-NA) - NO2- Triton X-100 - NaOH -乙醇体系。确定了该体系胶束饱和相形成的最佳条件:3.10-4 M 4-NA - 3.10-4 M NO2- 5% Triton X-100 - 2.8 M NaOH -10 vol. % С 2Н5ОН。对该体系进行了分光光度法研究。在坐标A (λ max = 552 nm)与r(百里香酚)之间建立了线性关系，其表达式为A = f(c)， A = 26291c + 0.02;R2 = 0.997。百里香酚的测定范围为(2·10-6 ~ 4·10-5)mol/l。建立了水介质中百里香酚的比色测定方法(色道G)。通道G色度(IG)的强度与pc(百里香酚)呈线性关系:IG = 54.2pc - 267, r2 = 0;百里香酚的测定下限为1.10 ~ 6 mol/l，比分光光度法测定的下限低2倍。构造了RGB CMYK模型颜色坐标下的花瓣图轮廓;得到了它们的面积(S)和周长(P)与百里香酚浓度的关系(P: y = 278x - 10.13;r2 = 0.97;S: y = 20182x - 87649, r2 = 0.99)。
{"title":"Determination of thymol with micellar extraction preconcentration","authors":"Elmira I. Tsygulyova, S. Doronin","doi":"10.18500/1816-9775-2021-21-3-267-273","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-267-273","url":null,"abstract":"For the efficient preconcentration of azo compounds – products of the interaction of 4-nitrophenyldiazonium with thymol, a system 4-nitroaniline (4-NA) – NO2- – Triton X-100 – NaOH – ethanol has been proposed. The optimal conditions for the formation of micellarsaturated phases of the system under study have been established: 3.10-4 M 4-NA – 3.10-4 M NO2- – 5% Triton X-100 – 2.8 M NaOH – 10 vol. % С 2Н5ОН. A spectrophotometric study of the above system has been carried out. A linear dependence was built in the coordinates A (at λ max = 552 nm) vs с(thymol), which is described by an equation of the form A = f(c), A = 26291c + 0.02; R2 = 0.997. The range of the determined contents of thymol is (2·10-6 – 4·10-5) mol/l. A technique for the colorimetric determination of thymol in aqueous media (color channel G) has been developed. The intensity of the channel G chromaticity (IG) is linearly dependent on pc(thymol) in accordance with the equation IG = 54.2pc – 267, R 2 = 0; the lower limit of the determined contents of thymol is 1.10-6 mol/l, which is two times less than in the variant of its spectrophotometric determination. The profiles of petal diagrams in the color coordinates of the RGB CMYK model have been constructed; the dependences of their area (S) and perimeter (P) on the thymol concentration have been obtained (P: y = 278x – 10.13; R 2 = 0.97; S: y = 20182x – 87649, R 2 = 0.99).","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88613543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-274-279
M. V. Chernykh, I. M. Ibrahim, V. Grinev, E. Sigida, Y. P. Fedonenko, S. Konnova
An extracellular polymer was isolated from the culture liquid of Bacillus subtilis EGP5QL12 with the yield of 5,6 g/L. On the basis of the data of thin layer chromatography, colorimetric analyses and FTIR spectroscopy, it was established that the polymer is poly-γ- glutamic acid (PGA). PGA is widely used in medicine, cosmetology and the food industry due to its ability to bind water and metal ions. To assess the biotechnological potential of the isolated polymer and predict the possibilities of its application in various fields of the national economy, it is necessary to analyze the characteristic spectral features that make it possible to establish its secondary structure. The isolated PGA preparation was analyzed by circular dichroism spectroscopy at various pH values. According to the results of this study, it was found that the polymer forms predominantly β-structures with a low proportion of irregular structures and α-helices, which gives it a high potential for creating hydrogels and composite materials.
{"title":"Evaluation of the secondary structure of poly-γ-glutamic acid produced by Bacillus subtilis EGP5QL12 by circular dichroism spectroscopy method","authors":"M. V. Chernykh, I. M. Ibrahim, V. Grinev, E. Sigida, Y. P. Fedonenko, S. Konnova","doi":"10.18500/1816-9775-2021-21-3-274-279","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-274-279","url":null,"abstract":"An extracellular polymer was isolated from the culture liquid of Bacillus subtilis EGP5QL12 with the yield of 5,6 g/L. On the basis of the data of thin layer chromatography, colorimetric analyses and FTIR spectroscopy, it was established that the polymer is poly-γ- glutamic acid (PGA). PGA is widely used in medicine, cosmetology and the food industry due to its ability to bind water and metal ions. To assess the biotechnological potential of the isolated polymer and predict the possibilities of its application in various fields of the national economy, it is necessary to analyze the characteristic spectral features that make it possible to establish its secondary structure. The isolated PGA preparation was analyzed by circular dichroism spectroscopy at various pH values. According to the results of this study, it was found that the polymer forms predominantly β-structures with a low proportion of irregular structures and α-helices, which gives it a high potential for creating hydrogels and composite materials.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81404512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-304-308
Victoriya S. Rastegaeva, E. Pleshakova
In today’s modern technogenic world, forming an ecological consciousness, perception and thinking among schoolchildren is a vital task. Knowledge of chemical toxicology is essential for students of all ages to help protect and maintain a healthy environment. Given that toxicology is characterized by an increased level of complexity, the purpose of this work was to develop and apply gaming methods in teaching the basics of chemical toxicology as part of environmental education of schoolchildren. We developed and implemented a training lesson for schoolchildren called “Fascinating phytotoxicology” for secondary school students, which consists of several stages. At the first stage, when using explanatory and illustrative methods in teaching, students received theoretical knowledge of phytotoxicology; at the second stage, students enhanced their knowledge in a playful way by solving situational problems composed by us, guessing riddles and answering intriguing questions; at the third stage, a survey was conducted among schoolchildren to identify the students’ level of interest in the topic and how much of the material was assimilated. The survey showed that the implementation of this gaming teaching method allowed students to learn complex material better, increased the activity and interest of students, and also contributed to the formation of an ecological awareness and environmental responsibility.
{"title":"The use of games as a method in teaching the basics of chemical toxicology as part of the ecological education of schoolchildren","authors":"Victoriya S. Rastegaeva, E. Pleshakova","doi":"10.18500/1816-9775-2021-21-3-304-308","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-304-308","url":null,"abstract":"In today’s modern technogenic world, forming an ecological consciousness, perception and thinking among schoolchildren is a vital task. Knowledge of chemical toxicology is essential for students of all ages to help protect and maintain a healthy environment. Given that toxicology is characterized by an increased level of complexity, the purpose of this work was to develop and apply gaming methods in teaching the basics of chemical toxicology as part of environmental education of schoolchildren. We developed and implemented a training lesson for schoolchildren called “Fascinating phytotoxicology” for secondary school students, which consists of several stages. At the first stage, when using explanatory and illustrative methods in teaching, students received theoretical knowledge of phytotoxicology; at the second stage, students enhanced their knowledge in a playful way by solving situational problems composed by us, guessing riddles and answering intriguing questions; at the third stage, a survey was conducted among schoolchildren to identify the students’ level of interest in the topic and how much of the material was assimilated. The survey showed that the implementation of this gaming teaching method allowed students to learn complex material better, increased the activity and interest of students, and also contributed to the formation of an ecological awareness and environmental responsibility.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76682537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-286-291
A. R. Bagavova, N. S. Velichko, Timofey E. Pylayev, Y. P. Fedonenko
The Herbaspirillum lusitanum P6-12 strain containing the vector plasmid pJN105TurboGFP, which encodes the synthesis of the green fluorescent protein GFP, and which has resistance to the antibiotic gentamicin, was obtained by electroporation. The constructed strain of H. lusitanum P6-12 in cultural, morphological and biochemical properties did not differ from the original typical natural strain of H. lusitanum P6-12. On solid growth media, the recombinant strain formed yellow-green colonies, fluorescent under UV irradiation. Upon inoculation with the resulting culture of plant objects, a green glow of the marked H. lusitanum P6-12 cells, actively colonizing the internal tissues of the host plant, was observed. The created strain can be used as a model strain for studying the patterns and characteristics of the behaviour of organisms in integrated systems, including for tracking bacterial cells during interaction with plants, assessing their survival, competitiveness, etc.
{"title":"Obtaining a fluorescently labeled endophytic strain of bacteria Herbaspirillum lusitanum P6-12 for their detection in vivo and in vitro","authors":"A. R. Bagavova, N. S. Velichko, Timofey E. Pylayev, Y. P. Fedonenko","doi":"10.18500/1816-9775-2021-21-3-286-291","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-286-291","url":null,"abstract":"The Herbaspirillum lusitanum P6-12 strain containing the vector plasmid pJN105TurboGFP, which encodes the synthesis of the green fluorescent protein GFP, and which has resistance to the antibiotic gentamicin, was obtained by electroporation. The constructed strain of H. lusitanum P6-12 in cultural, morphological and biochemical properties did not differ from the original typical natural strain of H. lusitanum P6-12. On solid growth media, the recombinant strain formed yellow-green colonies, fluorescent under UV irradiation. Upon inoculation with the resulting culture of plant objects, a green glow of the marked H. lusitanum P6-12 cells, actively colonizing the internal tissues of the host plant, was observed. The created strain can be used as a model strain for studying the patterns and characteristics of the behaviour of organisms in integrated systems, including for tracking bacterial cells during interaction with plants, assessing their survival, competitiveness, etc.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74103796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-324-327
Ilya S. Leontiev
The results of testing of the method of cultivation of the deadhead hawk moth Acherontia atropos (Linnaeus, 1758) using solid culture medium are given in this article. Optimal cultivation conditions for all caterpillar stages of this species are discussed. Information about bacterial infection of caterpillars which is caused by Gram-negative rod-shaped bacterial flora, is also noted in the results. Methods of treatment of this bacterial infection were selected empirically and also given use of bactericidal antibiotics (ceftriaxone) and selenopiran. We also trialled the use of a similar method of cultivation on some specific media for rare species of Lepidoptera like Proserpinus proserpina, Eudia pavonia, Saturnia pyri and some other species.
{"title":"Features of cultivation of the deadhead hawk moth Acherontia atropos (Linnaeus, 1758) on artificial nutrient media","authors":"Ilya S. Leontiev","doi":"10.18500/1816-9775-2021-21-3-324-327","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-324-327","url":null,"abstract":"The results of testing of the method of cultivation of the deadhead hawk moth Acherontia atropos (Linnaeus, 1758) using solid culture medium are given in this article. Optimal cultivation conditions for all caterpillar stages of this species are discussed. Information about bacterial infection of caterpillars which is caused by Gram-negative rod-shaped bacterial flora, is also noted in the results. Methods of treatment of this bacterial infection were selected empirically and also given use of bactericidal antibiotics (ceftriaxone) and selenopiran. We also trialled the use of a similar method of cultivation on some specific media for rare species of Lepidoptera like Proserpinus proserpina, Eudia pavonia, Saturnia pyri and some other species.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74721386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-24DOI: 10.18500/1816-9775-2021-21-3-298-303
A. V. Kovrizhnikov, T. Pylaev, A. M. Zaharevich, S. Konnova, M. Kupryashina
Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding. We demonstrated the retained respiratory activity and growth ability of the bacteria during immobilization, thus confirming the advantageous prospects of alginate templates for azospirilla encapsulation.
{"title":"Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel","authors":"A. V. Kovrizhnikov, T. Pylaev, A. M. Zaharevich, S. Konnova, M. Kupryashina","doi":"10.18500/1816-9775-2021-21-3-298-303","DOIUrl":"https://doi.org/10.18500/1816-9775-2021-21-3-298-303","url":null,"abstract":"Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding. We demonstrated the retained respiratory activity and growth ability of the bacteria during immobilization, thus confirming the advantageous prospects of alginate templates for azospirilla encapsulation.","PeriodicalId":14627,"journal":{"name":"Izvestiya of Saratov University. New Series. Series: Chemistry. Biology. Ecology","volume":"308 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77629182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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