{"title":"Differentiation of avirulent from virulent strains of human type tubercle bacilli by as (acid-sensitive) marker in vitro.","authors":"Masahiro Nakamura, M. Sasaki","doi":"10.3412/JSB.26.537","DOIUrl":"https://doi.org/10.3412/JSB.26.537","url":null,"abstract":"大阪大学微生物病研究所,東北大学抗酸菌病研究所および国立予防衛生研究所よりそれぞれヒト型結核菌H37 RvおよびH37 Ra株の分与をうけ,それらをpH 4.5小川培地に培養することによつて,この酸性培地に発育しうるか否か,すなわちas (acid-sensitive)マーカーにより強毒株H37 Rvと弱毒株H37 Raとの分別を試みて次の結果を得た。1. 通常の方法で保存されているH37 Rv株は例外なくpH 4.5小川培地に集落を生じas+であるのに反し,H37 Rv株はpH 4.5小川培地に集落を形成しえずas-であつた。2. しかし,H37 Rv株とH37 Ra株のpopulationの中には前者において約10% as-の混在があり,後者では約40%にas+の混在があつた。3. さらにH37 RvとH37 Raの原株から定型的asマーカーをもつ集落各1株を選びasマーカーを検討したところ,前者には約6%の割にas-,後者には約8%の割にas+の混在があった。4. 非定型的性格をもつH37 Rv株の集落as-とH37 Ra株の集落as+の中のpopulationをasマーカーで検討すると,前者にはなおも92%のas+の混在があり,後者ではas-は0%であつた。以上の結果より,ヒト型結核菌H37株はRv株,Ra株とも,その基盤はas+と判断される。しかしながら,H37の原株としての表現型はH37 Rv株はas+であり,H37 Ra株はas-である。asマーカーと病原性との相関関係およびas-の機作等については目下検討中である。","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"182 1","pages":"537-542"},"PeriodicalIF":0.0,"publicationDate":"1971-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76723796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Escherichia coli K-12 F- harboring R12 (CM. SM. SA) factor was stored in cooked meat medium at room temperature. After 3 years, it was examined for loss of R factor, as well as the genetic properties of this factor. It was found that 90.5% of surviving cells had lost their R factor to be sensitive to chloramphenicol (CM), streptomycin (SM), and sulfanilamide (SA). In the other surviving cells retaining their R factor, no segregation was noticed among resistance markers, So long as examined. Two derivative R factors, R12-5 and R12-22, were proveed to be nontransferable by conjugation, indicating that transferable (tra+) R12 factor had mutated to tra- R factor by storage in cooked meat medium for along time. From the results of a curing experiment and transduction to the rec- strain, it was clarified that the tra- R factors, R12-5 and R12-22, existed extrachromosomally and were replicated (rep+) autonomously in their host. Genetic studies revealed that R12-5 and R12-22 factors were ifm+ (inhibition of F-mating) and irs+ (interference of R factor superinfection), as well as the original R12 factor was. Reversion took place from tra- to tra+ at a frequency of 8.0 to 8.8×10-10, indicating that these were point mutants of the tra loci. The E. coli K-12 strain carrying either tra- R12-5 or tra- R12-22 acquired an ability to transfer its nontransferable R factor by complementation in cooperation with F factor, chromosomal F of the Hfr strain, or with transferable R factor.
{"title":"クックトミート培地に保存中に見られたR12(CM・SM・SA)因子の自然消失と非伝達性変異","authors":"隆勝 中嶋, 鈴木 ミツヱ, 三男 亀田, 賢治 原田, 進 三橋","doi":"10.3412/JSB.25.345","DOIUrl":"https://doi.org/10.3412/JSB.25.345","url":null,"abstract":"Escherichia coli K-12 F- harboring R12 (CM. SM. SA) factor was stored in cooked meat medium at room temperature. After 3 years, it was examined for loss of R factor, as well as the genetic properties of this factor. It was found that 90.5% of surviving cells had lost their R factor to be sensitive to chloramphenicol (CM), streptomycin (SM), and sulfanilamide (SA). In the other surviving cells retaining their R factor, no segregation was noticed among resistance markers, So long as examined. Two derivative R factors, R12-5 and R12-22, were proveed to be nontransferable by conjugation, indicating that transferable (tra+) R12 factor had mutated to tra- R factor by storage in cooked meat medium for along time. From the results of a curing experiment and transduction to the rec- strain, it was clarified that the tra- R factors, R12-5 and R12-22, existed extrachromosomally and were replicated (rep+) autonomously in their host. Genetic studies revealed that R12-5 and R12-22 factors were ifm+ (inhibition of F-mating) and irs+ (interference of R factor superinfection), as well as the original R12 factor was. Reversion took place from tra- to tra+ at a frequency of 8.0 to 8.8×10-10, indicating that these were point mutants of the tra loci. The E. coli K-12 strain carrying either tra- R12-5 or tra- R12-22 acquired an ability to transfer its nontransferable R factor by complementation in cooperation with F factor, chromosomal F of the Hfr strain, or with transferable R factor.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"34 1","pages":"345-349"},"PeriodicalIF":0.0,"publicationDate":"1970-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72718113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rabbit anti-thymic and anti-bursal lymphocyte sera (hereafter abbreviated to RATLS and RABLS, respectively) were examined for immunosuppressive effect on the hemolytic plaque-forming cells (PFC) and rosette-forming cells (RFC) in the spleen, and on experimental allergic encephalomyelitis (EAE) in chickens. The results obtained are as follows:1) RATLS anb RABLS had a high lymphoagglutinating activity on the thymic and bursal lymphocytes, respectively. In addition, a cross-reaction was observed between both anti-lymphocyte sera. This suggests that the thymic and bursal lymphocytes may have common antigen(s) in addition to the respective specific antigen(s) on the surface of the cell.2) Repeated administration of RATLS caused a more remarkable fall in lymphocyte count of the peripheral blood than that of RABLS. Furthermore, severe depletion of lymphocytes in the white pulp of the spleen was a characteristic change caused by RATLS, and a slight decrease in lymphocytes and pyroninophilic cells and the appearance of ill-defined germinal centers were ones induced by RABLS.3) In the primary response, when RABLS was injected daily for five days following the sensitization with sheep erythrocytes, there was a marked decrease in the number of PFC and RFC in the spleen. In contrast, such a suppression by RABLS was not so marked in the secondary response in previously immunized birds. On the other hand, RATLS was found to have a weak suppressive effect only in the primary response.4) The occurrence of EAE and the delayed-type hypersensitivity (wattle test) to the encephalitogenic basic protein of myelin were suppressed remarkably by the repeated injection of RATLS, but much less remarkably by the repeated administration of RABLS.From the findings obtained, it was ascertained that RATLS exerted a suppressive effect selectively on the cell-mediated immune reaction, and that RABLS did such an effect on the humoral antibody production. Therefore, both anti-lymphocyte sera were proved to have a considerably high selective activity in their immunosuppression.
{"title":"Studies on the Immunosuppressive Effects of Anti-thymic and Anti-bursal Lymphocyte Sera","authors":"K. Ohkuma","doi":"10.3412/JSB.25.305","DOIUrl":"https://doi.org/10.3412/JSB.25.305","url":null,"abstract":"Rabbit anti-thymic and anti-bursal lymphocyte sera (hereafter abbreviated to RATLS and RABLS, respectively) were examined for immunosuppressive effect on the hemolytic plaque-forming cells (PFC) and rosette-forming cells (RFC) in the spleen, and on experimental allergic encephalomyelitis (EAE) in chickens. The results obtained are as follows:1) RATLS anb RABLS had a high lymphoagglutinating activity on the thymic and bursal lymphocytes, respectively. In addition, a cross-reaction was observed between both anti-lymphocyte sera. This suggests that the thymic and bursal lymphocytes may have common antigen(s) in addition to the respective specific antigen(s) on the surface of the cell.2) Repeated administration of RATLS caused a more remarkable fall in lymphocyte count of the peripheral blood than that of RABLS. Furthermore, severe depletion of lymphocytes in the white pulp of the spleen was a characteristic change caused by RATLS, and a slight decrease in lymphocytes and pyroninophilic cells and the appearance of ill-defined germinal centers were ones induced by RABLS.3) In the primary response, when RABLS was injected daily for five days following the sensitization with sheep erythrocytes, there was a marked decrease in the number of PFC and RFC in the spleen. In contrast, such a suppression by RABLS was not so marked in the secondary response in previously immunized birds. On the other hand, RATLS was found to have a weak suppressive effect only in the primary response.4) The occurrence of EAE and the delayed-type hypersensitivity (wattle test) to the encephalitogenic basic protein of myelin were suppressed remarkably by the repeated injection of RATLS, but much less remarkably by the repeated administration of RABLS.From the findings obtained, it was ascertained that RATLS exerted a suppressive effect selectively on the cell-mediated immune reaction, and that RABLS did such an effect on the humoral antibody production. Therefore, both anti-lymphocyte sera were proved to have a considerably high selective activity in their immunosuppression.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"29 1","pages":"305-315"},"PeriodicalIF":0.0,"publicationDate":"1970-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87218185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Some Escherichia coli strains were isolated from 62 cattle, 4 sheep, and 14 goats, and surveyed for drug-resistance and distribution of R factors. Of the 62 cattle examined, 21 animals (34%) harbored drug-resistant E. coli strains, in which the resistance was transferable by conjugation. Drug-resistant E. coli strains were isolated neither from sheep nor from goats so far as examined. Of the drug-resistant strains, (TC, SM, SA)-resistant strains were isolated most frequently, and followed by (SM, SA)-resistant strains.In contrast, the isolation frequency of (TC, SM)-, (TC, SA)-, TC-, SM-and SA-resistant strains was less than 10%.Of 21 cattle carrying resistant E. coli strains, 9 animals (43%) excreted E. coli strains harboring R factors.A survey on drug-resistance patterns of R factors disclosed that (TC, SM, SA)-resistant R factors were isolated most frequently, and followed by (SM, SA)-, (TC, SA)-, TC- and SM-resistant R factors in a decreasing order.
{"title":"Demonstration of R Factors in Strains of Escherichia coli Isolated from Domestic Animals","authors":"Kaname Suzuki, S. Isogai, H. Hashimoto","doi":"10.3412/JSB.25.145","DOIUrl":"https://doi.org/10.3412/JSB.25.145","url":null,"abstract":"Some Escherichia coli strains were isolated from 62 cattle, 4 sheep, and 14 goats, and surveyed for drug-resistance and distribution of R factors. Of the 62 cattle examined, 21 animals (34%) harbored drug-resistant E. coli strains, in which the resistance was transferable by conjugation. Drug-resistant E. coli strains were isolated neither from sheep nor from goats so far as examined. Of the drug-resistant strains, (TC, SM, SA)-resistant strains were isolated most frequently, and followed by (SM, SA)-resistant strains.In contrast, the isolation frequency of (TC, SM)-, (TC, SA)-, TC-, SM-and SA-resistant strains was less than 10%.Of 21 cattle carrying resistant E. coli strains, 9 animals (43%) excreted E. coli strains harboring R factors.A survey on drug-resistance patterns of R factors disclosed that (TC, SM, SA)-resistant R factors were isolated most frequently, and followed by (SM, SA)-, (TC, SA)-, TC- and SM-resistant R factors in a decreasing order.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"7 1","pages":"145-148"},"PeriodicalIF":0.0,"publicationDate":"1970-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81798953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fifteen strains of Staphylococcus epidermidis were isolated from the blood of patients of septicemia. They produced white colonies, except two strains, and were negative for both coagulase and egg-yolk tests, indicating that they were of S. epidermidis in its literal meaning. Some of them were positive for both hemolysin and DNase (deoxyribonuclease) formation. When inoculated into mice by the subcutaneous or intraperitoneal route, all of them produced subcutaneous or intraperitoneal abscesses, except 4 strains. When inoculated by the intravenous route, 5 strains caused hemorrhagic or suppurative lesions in the kidneys, but none of the others induced lethal infection, except coagulase-positive strains used as controls.
{"title":"Studies on Staphylococcus epidermidis from Clinical Sources","authors":"M. Murakami","doi":"10.3412/JSB.25.591","DOIUrl":"https://doi.org/10.3412/JSB.25.591","url":null,"abstract":"Fifteen strains of Staphylococcus epidermidis were isolated from the blood of patients of septicemia. They produced white colonies, except two strains, and were negative for both coagulase and egg-yolk tests, indicating that they were of S. epidermidis in its literal meaning. Some of them were positive for both hemolysin and DNase (deoxyribonuclease) formation. When inoculated into mice by the subcutaneous or intraperitoneal route, all of them produced subcutaneous or intraperitoneal abscesses, except 4 strains. When inoculated by the intravenous route, 5 strains caused hemorrhagic or suppurative lesions in the kidneys, but none of the others induced lethal infection, except coagulase-positive strains used as controls.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"25 1","pages":"493-498"},"PeriodicalIF":0.0,"publicationDate":"1970-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77832066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yeast-like cells (blastospores) of Candida albicans and C. stellatoidea present two types of cell wall formation in the process of cell growth. One type occurs in the bud formation, and the other in the germination. The present paper deals with the mechanism of these types of cell wall formation at the electron microscopical level.Bud formation occurred mostly when the yeast-like cells were cultured on Sabouraud's agar medium. The bud wall was produced by extension of the whole layers of the mother cell wall. After the bud cell developed fully in size, as well as in contents, the septal wall was formed in it, first on the mother-cell side and then on the bud-cell side. It was characteristic of electron-dense granules to be seen associated with membranous structures around the newly formed septal wall. These results suggested some possible mechanism for the septal-wall formation.The septal wall may be produced by the accumulation of structural substances activated by the aid of these granules observed, mitochondria, and other membranous structures. It may not be produced by an inward extension of the cell wall, nor by simple fission.The germination of blastospores took place easily with a diversity of unknown environmental factors. After the outer wall ruptured in the germinating region, the inner most layer of the cell wall, which was thin and transparent in electron density, protruded to be thick, so that an intact wall might develop in the daughter cell.In an elongated germ-tube, septal walls were observed. They were similar in appearance to those of mycelial fungi.It is interesting to note that the same type of cells (blastospores) takes a different growth phase depending upon the kind of culture.
{"title":"Electron microscopic studies on the growth process of fungal cells. I. Nuclear division and accompanying behavior of mitochondria in the genus Candida","authors":"J. Tokunaga, M. Tokunaga, T. Egashira, K. Harada","doi":"10.3412/JSB.24.671","DOIUrl":"https://doi.org/10.3412/JSB.24.671","url":null,"abstract":"Yeast-like cells (blastospores) of Candida albicans and C. stellatoidea present two types of cell wall formation in the process of cell growth. One type occurs in the bud formation, and the other in the germination. The present paper deals with the mechanism of these types of cell wall formation at the electron microscopical level.Bud formation occurred mostly when the yeast-like cells were cultured on Sabouraud's agar medium. The bud wall was produced by extension of the whole layers of the mother cell wall. After the bud cell developed fully in size, as well as in contents, the septal wall was formed in it, first on the mother-cell side and then on the bud-cell side. It was characteristic of electron-dense granules to be seen associated with membranous structures around the newly formed septal wall. These results suggested some possible mechanism for the septal-wall formation.The septal wall may be produced by the accumulation of structural substances activated by the aid of these granules observed, mitochondria, and other membranous structures. It may not be produced by an inward extension of the cell wall, nor by simple fission.The germination of blastospores took place easily with a diversity of unknown environmental factors. After the outer wall ruptured in the germinating region, the inner most layer of the cell wall, which was thin and transparent in electron density, protruded to be thick, so that an intact wall might develop in the daughter cell.In an elongated germ-tube, septal walls were observed. They were similar in appearance to those of mycelial fungi.It is interesting to note that the same type of cells (blastospores) takes a different growth phase depending upon the kind of culture.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"18 1","pages":"671-675"},"PeriodicalIF":0.0,"publicationDate":"1969-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86574535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Increasing drug resistance and lowering phage typability are the characteristic features of staphylococci isolated at the Hokkaido University Hospital in the recent years. Of 285 strains obtained in 1966, only 105 (36.9%) were typable by means of the routine typing phages, with an occurrence of 63 strains (22.1%) with types 80/81. Seventy-seven of 180 phages derived from the untypable staphylococci lyzed one or more of the standard propagating strains, 46 (25.5%) of which were either PS 80 or PS 81. Cross lysis among the untypable staphylococci revealed 54 strains (30.0%) to be lysogenic.The phages from the untypable strains of staphylococci could be divided into the following three groups according to the host range. Two of the three groups were akin to each other and also to phages 80 and 81 (phages A and C); the third group was related to phage group III (phage B). Typing of the 180 untypable staphylococcal strains with these three phage varieties resulted in successful typing of 74 strains (41.1%), 70 of which were sensitive to the 80 and 81 related phages A and C. The fact suggests that a substantial portion of the untypable hospital strains of staphylococci may be lysogenized by phages related to phage 80 or 81 and hence phage resistant.In the present study, a close relationship was observed between the phage types of staphylococci and their drug resistance. Strains typable with the routine typing phages, except phages 80 and 81, were generally less resistant to ordinary antibiotics. Type 80/81, as well as the untypable strains of staphylococci, was more resistant, while such strains as lyzed by phages A and/or C were extremely resistant. The high and multiple drug resistance of the latter strains might have occurred through transduction by some special phages.
{"title":"ファージ型別不能,多剤耐性の病巣分離ブドウ球菌と,その誘発ファージについての研究","authors":"H. Yoshioka, Y. Satake, T. Murayama","doi":"10.3412/JSB.24.380","DOIUrl":"https://doi.org/10.3412/JSB.24.380","url":null,"abstract":"Increasing drug resistance and lowering phage typability are the characteristic features of staphylococci isolated at the Hokkaido University Hospital in the recent years. Of 285 strains obtained in 1966, only 105 (36.9%) were typable by means of the routine typing phages, with an occurrence of 63 strains (22.1%) with types 80/81. Seventy-seven of 180 phages derived from the untypable staphylococci lyzed one or more of the standard propagating strains, 46 (25.5%) of which were either PS 80 or PS 81. Cross lysis among the untypable staphylococci revealed 54 strains (30.0%) to be lysogenic.The phages from the untypable strains of staphylococci could be divided into the following three groups according to the host range. Two of the three groups were akin to each other and also to phages 80 and 81 (phages A and C); the third group was related to phage group III (phage B). Typing of the 180 untypable staphylococcal strains with these three phage varieties resulted in successful typing of 74 strains (41.1%), 70 of which were sensitive to the 80 and 81 related phages A and C. The fact suggests that a substantial portion of the untypable hospital strains of staphylococci may be lysogenized by phages related to phage 80 or 81 and hence phage resistant.In the present study, a close relationship was observed between the phage types of staphylococci and their drug resistance. Strains typable with the routine typing phages, except phages 80 and 81, were generally less resistant to ordinary antibiotics. Type 80/81, as well as the untypable strains of staphylococci, was more resistant, while such strains as lyzed by phages A and/or C were extremely resistant. The high and multiple drug resistance of the latter strains might have occurred through transduction by some special phages.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"42 1","pages":"380-386"},"PeriodicalIF":0.0,"publicationDate":"1969-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88411541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Many differential media have been devised for the identification of Shigellae, Salmonellae, and other enteric pathogens. None of them, however, had been designed for the determination of the activity of lysine decarboxylase, indole production, and motility of these organisms in a single tube of medium.The present investigation was undertaken with an objective to reduce the man-hour and to simplify the conventional tedious procedures when a large number of samples were involved. The medium developed in it met those criteria and was named lysine-indole-motility (LIM) medium.1) Composition and usage: LIM medium was composed of 10.0g of polypeptone (Daigo Eiyo Chemicals Co.), 3.0g of yeast extract (Difco), 1.0g of glucose, 10.0g of L-lysine monohydrochloride, 0.5g of L-tryptophan, 0.02g of bromcresol purple, 3.0g of agar, and 1, 000ml of distilled water. After these components were dissolved by heating at 100°C, pH was adjusted to 6.7±0.1. The resulting medium was dispensed into 10×100mm tubes in 3∼4ml amounts and sterilized at 121°C for 15 minutes. After the sterilization, the medium was cooled immediately by putting it into cold water. At use, organisms were picked up from a suspicious colony and stabbed into the middle of the medium. After 24 hours incubation at 37°C, reading was made.2) Results of comparative study: Comparison of results was made between LIM medium and some known differential media. A total of 120 Salmonella strains, 166 Shigella strains, and 87 strains of the other enteric pathogen were used. There was a complete agreement on results. Therefore, the sensitivity and accuracy of the medium were demonstrated. Furthermore, in routine diagnostic use, all the enteric pathogens could be tested easily with the new medium in combination with triple sugar iron (TSI) agar.
许多鉴别培养基已被设计用于鉴定志贺氏菌、沙门氏菌和其他肠道病原体。然而，没有一种方法被设计用于测定赖氨酸脱羧酶的活性、吲哚的产生和这些生物在单管培养基中的运动性。进行本调查的目的是在涉及大量样本时减少工时并简化传统的繁琐程序。其中培养的培养基符合上述标准，命名为赖氨酸-吲哚-活性(LIM)培养基。1)组成和使用:LIM培养基由10.0g多蛋白胨(Daigo Eiyo Chemicals Co.)、3.0g酵母浸膏(Difco)、1.0g葡萄糖、10.0g l -赖氨酸单盐酸、0.5g l -色氨酸、0.02g溴甲酚紫、3.0g琼脂和1000 ml蒸馏水组成。这些成分在100℃下加热溶解后，调整pH至6.7±0.1。将得到的培养基按3 ~ 4ml的量分配到10×100mm管中，在121°C下灭菌15分钟。灭菌后，立即将培养基放入冷水中冷却。在使用时，从可疑的菌落中取出生物并刺入培养基的中间。37℃孵育24小时后，进行读数。2)对比研究结果:将LIM培养基与一些已知的差示培养基进行结果比较。共检测沙门氏菌120株，志贺氏菌166株，其他肠道病原菌87株。结果是完全一致的。因此，证明了该介质的灵敏度和准确性。此外，在常规诊断中，新培养基与三糖铁(TSI)琼脂结合可方便地检测所有肠道病原体。
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