In order to analyse the mechanisms of host-parasite relationships in diphtherial infections, 4 strains of Corynebacterium diphtheriae isolated in Taiwan were first compared for their virulence by intradermal test in cortisone-treated and control (non-treated) groups of rabbits. It was found that #74 strain showed much weaker reaction as compared with other strains, i.e. #103, #78 and #69. This difference was apparent even after dilution of the inoculum to 10-1 or 10-2. In the dose-response curve, in which the amount of living bacilli (viable count) was taken into account, such difference in virulence was clearly observed. Therefore, the difference in virulence is not due to the difference in the number of viable bacilli present in each inoculum, but rather to the inherent ability of the individual bacillus itself to survive and produce toxin in the host.In the cortisone-treated groups of rabbits and guinea pigs, the skin reaction was generally markedly weaker than in control groups. The erythema at the site of injection was almost absent, and if present, it was observed to form a ring-form halo around the outer edge of induration. The induration was also less in size and thickness, and ulceration was rarely observed in the cortisone-treated groups.After intradermal injection of diphtheria bacilli, when the animals were not treated with a single injection of antitoxin to prevent premature death, the mortality of the infected rabbits and guinea pigs was not significantly different between the cortisone-treated and control groups. However, the positive rates of re-isolation of the diphtheria bacilli from regional lymph nodes and spleens of rabbits were significantly higher in cortisone treated than in control groups. Thus, the factors of parasite, i.e. inherent potency of the bacilli to survive and multiply in the host and toxin-producing ability, as well as the host factors such as amount of corticosterioids excreted, etc. seem to be the important determinants of diphtherial infection in man.
{"title":"Studies on Corynebacterium diphtheriae Isolated in Taiwan","authors":"Wen-Fun Kuo","doi":"10.3412/JSB.20.651","DOIUrl":"https://doi.org/10.3412/JSB.20.651","url":null,"abstract":"In order to analyse the mechanisms of host-parasite relationships in diphtherial infections, 4 strains of Corynebacterium diphtheriae isolated in Taiwan were first compared for their virulence by intradermal test in cortisone-treated and control (non-treated) groups of rabbits. It was found that #74 strain showed much weaker reaction as compared with other strains, i.e. #103, #78 and #69. This difference was apparent even after dilution of the inoculum to 10-1 or 10-2. In the dose-response curve, in which the amount of living bacilli (viable count) was taken into account, such difference in virulence was clearly observed. Therefore, the difference in virulence is not due to the difference in the number of viable bacilli present in each inoculum, but rather to the inherent ability of the individual bacillus itself to survive and produce toxin in the host.In the cortisone-treated groups of rabbits and guinea pigs, the skin reaction was generally markedly weaker than in control groups. The erythema at the site of injection was almost absent, and if present, it was observed to form a ring-form halo around the outer edge of induration. The induration was also less in size and thickness, and ulceration was rarely observed in the cortisone-treated groups.After intradermal injection of diphtheria bacilli, when the animals were not treated with a single injection of antitoxin to prevent premature death, the mortality of the infected rabbits and guinea pigs was not significantly different between the cortisone-treated and control groups. However, the positive rates of re-isolation of the diphtheria bacilli from regional lymph nodes and spleens of rabbits were significantly higher in cortisone treated than in control groups. Thus, the factors of parasite, i.e. inherent potency of the bacilli to survive and multiply in the host and toxin-producing ability, as well as the host factors such as amount of corticosterioids excreted, etc. seem to be the important determinants of diphtherial infection in man.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"26 1","pages":"651-657"},"PeriodicalIF":0.0,"publicationDate":"1965-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75558492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A purified coagulase was obtained from a culture filtrate of Staphylococcus aureus St-213 which proved to be homogenous in immunological, electrophoretical and Spinco Model E ultracentrifugal studies. Treatment of the material with trichloroacetic acid prior to passing through Dowex-1 and Diethylaminoethyl-Sephadex A-50 columns yielded a product which showed a 280 fold increase in activity per mg of nitrogen than the crude culture filtrate. The sedimentation coefficient of the treated material was S20, w=1.59S.The treated material was composed of 9 amino acids (i.e. lysin, aspartate, threonine, serine, glutamine, glycine, alanine, valine and methionine) and lacked carbohydrates, lipids and phosphates. Therefore, this purified material may be classified as a purified protein derivative (PPD) capable of exhibiting a specific coagulase type reaction against homologous and heterologous type antiserum when tested immunologically using the agar gel diffusion technique.
从金黄色葡萄球菌St-213培养滤液中获得纯化的凝固酶，经免疫学、电泳和Spinco模型E超离心研究证明其均质。在通过Dowex-1和diethylamino乙基- sephadex a -50色谱柱之前，用三氯乙酸处理该材料，所得产品每毫克氮的活性比粗培养滤液增加280倍。处理后物料的沉降系数为S20, w=1.59S。处理后的材料由9种氨基酸组成(即溶血素、天冬氨酸、苏氨酸、丝氨酸、谷氨酰胺、甘氨酸、丙氨酸、缬氨酸和蛋氨酸)，不含碳水化合物、脂类和磷酸盐。因此，当使用琼脂凝胶扩散技术进行免疫测试时，该纯化材料可被归类为纯化蛋白衍生物(PPD)，能够对同源和异源型抗血清表现出特定的凝固酶型反应。
{"title":"Studies on the Purification of Staphylococcal Coagulase and Its Properties","authors":"H. Hitokoto","doi":"10.3412/JSB.20.627","DOIUrl":"https://doi.org/10.3412/JSB.20.627","url":null,"abstract":"A purified coagulase was obtained from a culture filtrate of Staphylococcus aureus St-213 which proved to be homogenous in immunological, electrophoretical and Spinco Model E ultracentrifugal studies. Treatment of the material with trichloroacetic acid prior to passing through Dowex-1 and Diethylaminoethyl-Sephadex A-50 columns yielded a product which showed a 280 fold increase in activity per mg of nitrogen than the crude culture filtrate. The sedimentation coefficient of the treated material was S20, w=1.59S.The treated material was composed of 9 amino acids (i.e. lysin, aspartate, threonine, serine, glutamine, glycine, alanine, valine and methionine) and lacked carbohydrates, lipids and phosphates. Therefore, this purified material may be classified as a purified protein derivative (PPD) capable of exhibiting a specific coagulase type reaction against homologous and heterologous type antiserum when tested immunologically using the agar gel diffusion technique.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"17 1","pages":"627-633"},"PeriodicalIF":0.0,"publicationDate":"1965-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74121354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Sakaguchi, Sigeo Suzuki, Masuko Suzuki, H. Sunayama
From acetone-dried cells of C. albicans, we extracted the crude antigen with phosphate buffer solution (pH 7.2) containing phenol at concentrations of 45% at ordinary temperature (Fraction I), and from “cell. residue” with hot-water at 100 (Fraction II), and then further obtained a alkali soluble polysaccharide by use of 3% NaOH-extraction method under an atmosphere of N2 from insoluble “cell-residue” (Fraction III).The resulting crude extracts has been fractionated repeatedly with DEAE-Sephadex column, and a clarified Candida-mannan which showed a ultracentrifugically and electrophoretically single peak was obtained. In the fractions eluted from the column, there are two components of neutral and acidity fraction, and it has been ascertained that the later comprise a small amount of phosphorus.Each crude fractions of F. I, II, III showed nearly similar activities on precipitin reaction, and a decrease of their precipitin titer on account of drastic extraction methods was not observed.When Candida anti-serum was selectively absorbed with F. III-mannan, it was observed that the precipitin reaction of crude F. III expressed completely negative reaction and the precipitin titers were largely effected on their serological activities in the case of FI, II.
{"title":"Immunochemical and Biochemical Studies of Fungi (4)","authors":"O. Sakaguchi, Sigeo Suzuki, Masuko Suzuki, H. Sunayama","doi":"10.3412/JSB.20.693","DOIUrl":"https://doi.org/10.3412/JSB.20.693","url":null,"abstract":"From acetone-dried cells of C. albicans, we extracted the crude antigen with phosphate buffer solution (pH 7.2) containing phenol at concentrations of 45% at ordinary temperature (Fraction I), and from “cell. residue” with hot-water at 100 (Fraction II), and then further obtained a alkali soluble polysaccharide by use of 3% NaOH-extraction method under an atmosphere of N2 from insoluble “cell-residue” (Fraction III).The resulting crude extracts has been fractionated repeatedly with DEAE-Sephadex column, and a clarified Candida-mannan which showed a ultracentrifugically and electrophoretically single peak was obtained. In the fractions eluted from the column, there are two components of neutral and acidity fraction, and it has been ascertained that the later comprise a small amount of phosphorus.Each crude fractions of F. I, II, III showed nearly similar activities on precipitin reaction, and a decrease of their precipitin titer on account of drastic extraction methods was not observed.When Candida anti-serum was selectively absorbed with F. III-mannan, it was observed that the precipitin reaction of crude F. III expressed completely negative reaction and the precipitin titers were largely effected on their serological activities in the case of FI, II.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"12 1","pages":"693-699"},"PeriodicalIF":0.0,"publicationDate":"1965-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76822028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1. The process of toxin production was investigated withClostridium botulinumtype A, strain 190 and type B, strain Lamanna, both belonging to the proteolytic group ofClostridium botulinum. In theformer, no increase in the toxicity was observed after the trypsin treatment. However, in the latter, the toxicity increased by about 10 to 100 times with trypsin even in the late stage of incubation.2. The similar experiments were carried out withClostridium botulinumtype C, strain Stockholm, and type E, strain VH, both belonging to the non-proteolytic group ofClostridium botulinum. In the former, no distinct increase in the toxicity was observed after the trypsin treatment. Onthe contrary, the toxicity increased by about 100 to 1, 000 times with trypsin in the latter.3. The activation phenomenon ofClostridinum botulinumtoxin by trypsin seems to have no direct relationship with the proteolytic character of the strain.
{"title":"Clostridium botulinumの毒素産生に関する実験的研究 (第1報)","authors":"Hiroo Iida","doi":"10.3412/JSB.19.458","DOIUrl":"https://doi.org/10.3412/JSB.19.458","url":null,"abstract":"1. The process of toxin production was investigated withClostridium botulinumtype A, strain 190 and type B, strain Lamanna, both belonging to the proteolytic group ofClostridium botulinum. In theformer, no increase in the toxicity was observed after the trypsin treatment. However, in the latter, the toxicity increased by about 10 to 100 times with trypsin even in the late stage of incubation.2. The similar experiments were carried out withClostridium botulinumtype C, strain Stockholm, and type E, strain VH, both belonging to the non-proteolytic group ofClostridium botulinum. In the former, no distinct increase in the toxicity was observed after the trypsin treatment. Onthe contrary, the toxicity increased by about 100 to 1, 000 times with trypsin in the latter.3. The activation phenomenon ofClostridinum botulinumtoxin by trypsin seems to have no direct relationship with the proteolytic character of the strain.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"4 1","pages":"458-461"},"PeriodicalIF":0.0,"publicationDate":"1964-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79386998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There were many studies for Vibrio parahaemolyticus since it was discovered by Fujino et al in 1950.The author has also studied to clarify the properties of this organism, and found that this organism had a toxic component in its thick envelope. The slime layer of the envelope was isolated and purified. The purified fraction was found to be toxic to mice. The LD50 of the toxic fraction by intraperitoneal injection was about 70 mcg per mouse weighing of 15 gms. This toxic fraction which have a peak at 260 m & mu; in optical density was not inactivated with neither trypsin nor RNase, and its toxicity to mice lost by the enzymatic action of DNase. From the physico-chemical examination, the isolated toxic fraction might be almost purified DNA. These results suggest that the envelope substance of Vibrio parahaemolyticus might have a significant role and its toxic component might be DNA of the organism.
自1950年Fujino等人发现副溶血性弧菌以来，对其进行了大量的研究。作者还研究了澄清该生物的性质，并发现该生物在其厚包膜中含有有毒成分。对包膜的黏液层进行分离纯化。纯化后的部分被发现对小鼠有毒。腹腔注射毒性部分的LD50约为70 mcg /体重15 gms的小鼠。毒性部分在260 m & mu处达到峰值;胰蛋白酶和脱氧核糖核酸酶均不能灭活光密度，其对小鼠的毒性因脱氧核糖核酸酶的酶促作用而丧失。从理化检查来看，分离的毒性部分可能几乎是纯化的DNA。这些结果表明，副溶血性弧菌的包膜物质可能起重要作用，其毒性成分可能是该菌的DNA。
{"title":"[STUDIES ON THE BIOLOGICAL AND IMMUNOLOGICAL SIGNIFICANCE OF THE NUCLEIC ACIDS OF ENTERITIS-CAUSING VIBRIO. 1. THE PURIFICATION AND CHEMICAL PROPERTIES OF THE NUCLEAR AND TOXIC PORTION OF THE ENTERITIS-CAUSING VIBRIO].","authors":"K. Kuriyama","doi":"10.3412/JSB.19.418","DOIUrl":"https://doi.org/10.3412/JSB.19.418","url":null,"abstract":"There were many studies for Vibrio parahaemolyticus since it was discovered by Fujino et al in 1950.The author has also studied to clarify the properties of this organism, and found that this organism had a toxic component in its thick envelope. The slime layer of the envelope was isolated and purified. The purified fraction was found to be toxic to mice. The LD50 of the toxic fraction by intraperitoneal injection was about 70 mcg per mouse weighing of 15 gms. This toxic fraction which have a peak at 260 m & mu; in optical density was not inactivated with neither trypsin nor RNase, and its toxicity to mice lost by the enzymatic action of DNase. From the physico-chemical examination, the isolated toxic fraction might be almost purified DNA. These results suggest that the envelope substance of Vibrio parahaemolyticus might have a significant role and its toxic component might be DNA of the organism.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"1 1","pages":"418-424"},"PeriodicalIF":0.0,"publicationDate":"1964-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73544244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
1. A antigenic substance was isolated from the heated cell extract of B. anthracis through trichloroacetic acid treatment, the removal of lipids by ether, aceton-, ammonium sulfate-fractionation and zone electrophoresis.2. The substance was shown to be a single component by zone electrophoresis, ultracentrifugation and agar diffusion test.3. The precipitating titer of the substance for anti-anthracis serum was positive in 2, 048×103fold dilution.4. The substance was nonspecific in the sense that it reacted with anti-B. megatherium (B. cereus) and anti-B. subtilis serum.5. The substance was positive in molisch and anthrone reaction and negative in protein and nucleic acid reaction; thus it was to be polysaccharide.
{"title":"Studies on the Immunospecific and Non-specific Substance of Bacillus anthracis","authors":"T. Baba","doi":"10.3412/JSB.18.455","DOIUrl":"https://doi.org/10.3412/JSB.18.455","url":null,"abstract":"1. A antigenic substance was isolated from the heated cell extract of B. anthracis through trichloroacetic acid treatment, the removal of lipids by ether, aceton-, ammonium sulfate-fractionation and zone electrophoresis.2. The substance was shown to be a single component by zone electrophoresis, ultracentrifugation and agar diffusion test.3. The precipitating titer of the substance for anti-anthracis serum was positive in 2, 048×103fold dilution.4. The substance was nonspecific in the sense that it reacted with anti-B. megatherium (B. cereus) and anti-B. subtilis serum.5. The substance was positive in molisch and anthrone reaction and negative in protein and nucleic acid reaction; thus it was to be polysaccharide.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"62 1","pages":"121-124"},"PeriodicalIF":0.0,"publicationDate":"1964-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88981753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
By means of ultrathin section and electron microscopic technic the fine structures ofCandida albicansin yeast phase grown on Sabouraud's dextrose agar were revealed. Fixation of specimens proved to be superior in revealing the fine structures using a 2 % potassium permanganate aqueous solution (at room temperature for 48 hours) than with an osmic acid solution. Embedding was done in a routine method. The results obtained were as follows:The cell wall, relatively thick, consists of the three layers, two electron less dense ones divided by a dense interspace. The cytoplasmic membrane, very thin, is recognized.The nuclear apparatus is surrounded by a continous double-membraneous layer with pores; The nucleolus-like structures are observed as an electron dense region.The mitochondria with cristae mitochondriales are also surrounded by a continous double-membraneous layer as in the nucleus.The endoplasmic reticulum seems to be connected with the outer layers of both nucleus and mitochondria. The structures resembling the lamella, potassium permanganophilic uniform granules and vacuoles are also observed inside the cytoplasm.
{"title":"ELECTRON MICROSCOPE STUDIES ON THE STRUCTURES OF PATHOGENIC FUNGI. I. ON THE STRUCTURE OF ULTRATHIN SECTIONS OF CANDIDA ALBICANS","authors":"K. Iwata, T. Hirata","doi":"10.3412/JSB.18.393","DOIUrl":"https://doi.org/10.3412/JSB.18.393","url":null,"abstract":"By means of ultrathin section and electron microscopic technic the fine structures ofCandida albicansin yeast phase grown on Sabouraud's dextrose agar were revealed. Fixation of specimens proved to be superior in revealing the fine structures using a 2 % potassium permanganate aqueous solution (at room temperature for 48 hours) than with an osmic acid solution. Embedding was done in a routine method. The results obtained were as follows:The cell wall, relatively thick, consists of the three layers, two electron less dense ones divided by a dense interspace. The cytoplasmic membrane, very thin, is recognized.The nuclear apparatus is surrounded by a continous double-membraneous layer with pores; The nucleolus-like structures are observed as an electron dense region.The mitochondria with cristae mitochondriales are also surrounded by a continous double-membraneous layer as in the nucleus.The endoplasmic reticulum seems to be connected with the outer layers of both nucleus and mitochondria. The structures resembling the lamella, potassium permanganophilic uniform granules and vacuoles are also observed inside the cytoplasm.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"52 1","pages":"393-404"},"PeriodicalIF":0.0,"publicationDate":"1963-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86685787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Studies on the Nutritional Requirements of Bacteria XIII","authors":"E. Takemori, D. Mizuno","doi":"10.3412/JSB.18.375","DOIUrl":"https://doi.org/10.3412/JSB.18.375","url":null,"abstract":"内外の肉エキス, 酵母エキス, 肉汁約40種につき, 諸種ビタミン量をも測定した。酵母エキスは, 再現性は高いがややビタミン量に欠け, 肉エキスはビタミン量は充分だが, 再現性にかけることを観察した。結局半年間, の貯蔵に耐えた肉汁が, もつともいいビタミン源となり得るものと考察した。","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"113 2","pages":"375-378"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91423040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Ushiba, Kazuhisa Saito, M. Nakano, T. Akiyama, Y. Kishimoto, F. Okitsu, T. Saito
The intracellular growth of various species of salmonellae was tested by the roller tube technique using cultured peritoneal macrophages from DK1 strain of mice which were selectively inbred by a uniform susceptibility to S. enteritidis infection. Intracellular growth was observed markedly with S. enteritidis, S. typhimurium and S. choleraesuis, but not at all with S. paratyphi A, S. typhosa (V and VW types), and also with Escherichia coli (0-55 type). Some strains of S. paratyphi B showed a slight intracellular growth when large numbers of phagocytes were examined. When those strains of S. paratyphi B were intraperitoneally inoculated into DK1 mice, large numbers of organisms were harbored in the peritoneal fluid and organs through the 3rd week compared with other strains of the same species and S. paratyphi A or S. typhosa. Histopathological findings of the liver of inoculated mice showed the formation of granulomas or typhoms, large and small, in the degree almost parallel to the number of harbored organisms. The coincidence between the apparent intracellular growth of bacterial species and their capability to produce typhoid infection in mice was emphasized.
{"title":"Relationship between the Intracellular Multiplication of Salmonellae in Cultured Mononuclear Phagocytes and their Pathogenicity for the Host","authors":"D. Ushiba, Kazuhisa Saito, M. Nakano, T. Akiyama, Y. Kishimoto, F. Okitsu, T. Saito","doi":"10.3412/JSB.18.38","DOIUrl":"https://doi.org/10.3412/JSB.18.38","url":null,"abstract":"The intracellular growth of various species of salmonellae was tested by the roller tube technique using cultured peritoneal macrophages from DK1 strain of mice which were selectively inbred by a uniform susceptibility to S. enteritidis infection. Intracellular growth was observed markedly with S. enteritidis, S. typhimurium and S. choleraesuis, but not at all with S. paratyphi A, S. typhosa (V and VW types), and also with Escherichia coli (0-55 type). Some strains of S. paratyphi B showed a slight intracellular growth when large numbers of phagocytes were examined. When those strains of S. paratyphi B were intraperitoneally inoculated into DK1 mice, large numbers of organisms were harbored in the peritoneal fluid and organs through the 3rd week compared with other strains of the same species and S. paratyphi A or S. typhosa. Histopathological findings of the liver of inoculated mice showed the formation of granulomas or typhoms, large and small, in the degree almost parallel to the number of harbored organisms. The coincidence between the apparent intracellular growth of bacterial species and their capability to produce typhoid infection in mice was emphasized.","PeriodicalId":14812,"journal":{"name":"Japanese journal of bacteriology","volume":"18 13 1","pages":"38-43"},"PeriodicalIF":0.0,"publicationDate":"1963-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79822967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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