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Studying the Effect of Partially Purified ArthrobacterB1.Polysaccharide on Nucleosomal Distribution of HepG2 Tumor Cell Line 部分纯化关节细菌b1的作用研究。多糖对HepG2肿瘤细胞系核小体分布的影响
Pub Date : 2017-09-01 DOI: 10.22401/JNUS.17.3.23
Ali Z. Al-Saffar, Nawar B. Al-Roubaie, A. Wahid, B. Al-Shaibani
This study was designed to investigate the effect of partially purified polysaccharide extracted from ArthrobacterB1 on the DNA banding pattern of HepG2 tumor cell line invitro. The polysaccharide was extracted from Arthrobacter B1using phenol sulfuric acid methodwith a recovery yield of 181mg. Partial purification of polysaccharide was performed by using sepharose Cl-6B gel filtration chromatography and two peaks were obtained. Chemical analysis involving the estimation of carbohydrate and protein contents which showed that the large first peak contained the higher carbohydrate contents (30.1%) as compared with the crude polysaccharide 15.9% with low protein constituents (5.7%).The effect of Arthrobacter B1polysaccharide on HepG2 tumor cells was examined by studying whether bacterial polysaccharide participating in affecting the structure of tumor cell’s DNA or not. Results indicated that only intact nucleosomal DNA bands were separated on the gel for the untreated cells as compared with the banding pattern of the colchicine treated DNA, in which besides to intact nucleosomal DNA, mononucleosomes, dinucleosomes and oligonucleosomes were separated on the gel following electrophoresis process. Such pattern does not affected by the gradual increase in MNase concentration. On the other hand, the DNA banding pattern for the cells treated with Arthrobacter B1 polysaccharide showed significant effect by the separation of nucleosomal DNA, in which mononucleosomes, dinucleosomes and oligonucleosomes were clearly separated from intact nucleosomal DNA, and such effect was markedly appeared by increasing the concentration of MNase as compared with the cells treated colchicine.
本研究旨在探讨部分纯化的关节杆菌b1多糖对HepG2肿瘤细胞系DNA带型的体外影响。采用苯酚硫酸法从节杆菌b1中提取多糖,回收率为181mg。用蔗糖Cl-6B凝胶过滤层析法对多糖进行部分纯化,得到两个峰。化学分析包括碳水化合物和蛋白质含量的估计,结果表明,第一个峰的碳水化合物含量较高(30.1%),而粗多糖的碳水化合物含量为15.9%,蛋白质含量较低(5.7%)。通过研究细菌多糖是否参与影响肿瘤细胞DNA结构,考察关节杆菌b1多糖对HepG2肿瘤细胞的作用。结果表明,与秋水仙碱处理的DNA条带模式相比,未处理的细胞在凝胶上只分离出完整的核小体DNA条带,电泳过程中除了完整的核小体DNA外,凝胶上还分离出单核小体、二核小体和寡核小体。这种模式不受MNase浓度逐渐增加的影响。另一方面,关节杆菌B1多糖处理细胞的DNA带带模式受到核小体DNA分离的显著影响,单个核小体、二核小体和寡核小体与完整的核小体DNA明显分离,且与秋水珠碱处理细胞相比,增加MNase浓度可以明显地表现出这种影响。
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引用次数: 0
Potential Activity of Zinc Oxide Nanoparticles and Ethanolic Olive Leaf Extract Against Oxacillin Resistant Staphylococcus aureusin vitro 氧化锌纳米颗粒和乙醇橄榄叶提取物体外抗耐氧西林金黄色葡萄球菌的潜在活性
Pub Date : 2017-09-01 DOI: 10.22401/JNUS.17.3.22
Dhafar N. Al-ugaili, A. M. A. Fadhil, S. A. Wohaieb
This study was carried out to evaluate the enhanced effect of ZnO nanoparticles and a crude ethanolic extract of olive leaves upon the antibacterial activity of oxacillin against oxacillin resistant Staphylococcus aureus (ORSA) isolate from hospital environment. A positive and induced effect for ZnO nanoparticle was detected upon the antibacterial activity of the antibiotic through the increase in the diameter of the inhibition zone for the antibiotic as it recorded (15mm) for the mixed activity to the antibiotic with nanoparticles compared with that of (10mm) for the zinc oxide nanoparticle a lone and the disappearance of the inhibition zone for the antibiotic alone. The antibacterial efficacy of the crude ethanolic olive leaf extract was evaluated alone or in combination with either oxacillin or zinc oxide nanoparticles against oxacillin resistant Staphylococcus aureus using disc diffusion method. The results revealed that olive leaf extracts (2.25-9 mg/disc) neither exhibited antibacterial activity when used alone, nor potentiated the antibacterial activity of zinc oxide nanoparticles (100μg/disc) when used in combination. However, in the presence of the highest concentration of olive leaf extract (9mg/disc), the antibacterial activity of oxacillin (1μg/disc) (as a diameter of inhibition zone) increased from zero mm to a maximum of 14 mm, suggesting a potentiation of the antimicrobial activity of oxacillin against the already existing oxacillin-resistant strain of Staphylococcus aureus.
研究了氧化锌纳米颗粒和橄榄叶粗乙醇提取物对oxacillin对医院环境中耐oxacillin金黄色葡萄球菌(ORSA)的抗菌活性的增强作用。氧化锌纳米颗粒对抗生素的抑菌活性产生了积极的诱导作用,与氧化锌纳米颗粒对抗生素的抑菌区直径(10mm)相比,氧化锌纳米颗粒对抗生素的混合抑菌区直径增加(15mm),并且对抗生素的单独抑菌区消失。采用圆盘扩散法评价橄榄叶粗乙醇提取物单独或与氧苄西林或氧化锌纳米颗粒联合使用对耐氧苄西林金黄色葡萄球菌的抑菌效果。结果表明,橄榄叶提取物(2.25 ~ 9 mg/盘)单独使用时不表现出抗菌活性,与氧化锌纳米颗粒(100μg/盘)联合使用时也不增强其抗菌活性。然而,当橄榄叶提取物浓度最高(9mg/片)时,oxacillin (1μg/片)的抑菌活性(以抑菌带直径为单位)从0 mm增加到最大14 mm,这表明oxacillin对已经存在的耐oxacillin金黄色葡萄球菌的抑菌活性增强。
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引用次数: 1
التقدير الكمي والتنقية الجزئية لمركبي الفنبالستين و الفنكرستين لثالثة نباتات من العائلة الدفلية Apocynaceae 对芬巴利斯坦号和芬克斯汀号的第三类植物的定量和部分纯度
Pub Date : 2017-09-01 DOI: 10.22401/JNUS.17.3.34
صباح مهدي هادي, واثق عباس حتيت
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引用次数: 0
Chebyshev Wavelets Method for Solving Partial Differential Equations of Fractional Order 求解分数阶偏微分方程的切比雪夫小波方法
Pub Date : 2017-09-01 DOI: 10.22401/JNUS.17.3.25
O. H. Mohammed, Haneen A. Ameen
In this paper, we use the second kind Chebyshev wavelet operational matrix of fractional integration for solving fractional order linear partial differential equations.By using this method the fractional order partial differential equation is translated into Lyapunov type matrix equation and the computation effort become convenient. Two illustrative examples are provided to demonstrate the validity, simplicity and applicability of the numerical scheme based on the Chebyshev set of functions.
本文利用分数阶积分的第二类Chebyshev小波运算矩阵求解分数阶线性偏微分方程。该方法将分数阶偏微分方程转化为Lyapunov型矩阵方程,简化了计算过程。通过两个实例说明了基于切比雪夫函数集的数值格式的有效性、简便性和适用性。
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引用次数: 0
A Microbial Survey of Second Hand Clothe Samples Collected from Baghdad Market 巴格达市场二手衣物样品微生物调查
Pub Date : 2017-09-01 DOI: 10.22401/JUNS.20.3.19
Nada Abdulrahman F. Al-Easawi, Faiza kadhim Emran
Second hand represent one of the sources of illness due to environmentally related disease and injury. Used clothes are sold all over the country since they are very cheap compared with the new ones. The current study included collecting samples from different used clothing markets in Baghdad and comparing the efficiency of detergents used to reduce microbial load in the garments. The samples were adult's clothes, babies' clothes, and fabric toys. Isolation and identification of bacteria with CFU were done to determine which cloth samples carry the highest bacterial contamination and dominant pathogenic microbes associated with skin infection. Culture and biochemical methods were used for the identification. Samples were washed using two types of detergents (soap, granulated detergent) to investigate the efficiency of these detergents in reducing bacterial contamination in such clothes. The results showed that the dominant isolate bacteria in adult clothes were Staphylococcus sp. and Enterobacteriace sp. while Enterobacteriace sp., Bacillus sp. in baby clothes and Bacillus sp. in fabric toys. The results of CFU found to be the highest in baby's clothes (4x10 6 ), fabric toys (2.2x10 4 ), adult clothes (20x10 2 )respectively. Results showed that the isolated fungi were Aspergilus flavus, Aspergilus niger, Rhizopus. After washing with detergents including soap and granulated detergent, the results showed that the granulated detergent is more effective in reducing bacterial and fungal contamination than soap in all samples. This study concluded that babies' cloths are vulnmrable to contaminating with bacteria and fungi then fabric toys followed by adult clothes. It's recommended to wash second hand clothes using other methods to remove both bacterial and fungal contamination such as disinfectants then ironing. [DOI: 10.22401/JUNS.20.3.19]
由于与环境有关的疾病和伤害,二手代表了疾病的来源之一。全国各地都有卖旧衣服,因为它们比新衣服便宜。目前的研究包括从巴格达不同的二手服装市场收集样本,并比较用于减少服装微生物负荷的洗涤剂的效率。这些样品是成人衣服、婴儿衣服和织物玩具。用CFU对细菌进行了分离和鉴定,以确定哪些布料样品携带最高的细菌污染和与皮肤感染相关的主要致病微生物。采用培养法和生化法进行鉴定。使用两种类型的洗涤剂(肥皂,颗粒洗涤剂)洗涤样品,以研究这些洗涤剂减少此类衣服上细菌污染的效率。结果表明,成人服装中分离出的优势菌为葡萄球菌和肠杆菌,婴儿服装中分离出的优势菌为肠杆菌、芽孢杆菌和织物玩具中分离出的优势菌。CFU在婴儿服装(4 × 10 6)、织物玩具(2.2 × 10 4)和成人服装(20 × 10 2)中含量最高。结果表明,分离得到的真菌有黄曲霉、黑曲霉、根霉。用肥皂和颗粒状洗涤剂洗涤后,结果表明,在所有样品中,颗粒状洗涤剂比肥皂更有效地减少细菌和真菌污染。这项研究的结论是,婴儿的衣服最容易被细菌和真菌污染,其次是织物玩具,其次是成人的衣服。建议用其他方法洗二手衣服来去除细菌和真菌污染,比如用消毒剂,然后熨烫。(DOI: 10.22401 / JUNS.20.3.19)
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引用次数: 1
Evaluation the Corrosion of Mild Steel Rings in Simulated Conditions of Natural Gas Transportation 模拟天然气输送条件下低碳钢环的腐蚀评价
Pub Date : 2017-09-01 DOI: 10.22401/JUNS.20.3.08
Jewad K. Shneine
Corrosion of steel rings (steel tubes) under natural gas production conditions has been studied in a 2 L glass autoclave. Steel tubes, axially constructed with cavities of aspect ratios 3×1 mm, were exposed to CO2 saturated chloride salt solutions in a glass autoclave for 4 days at a flow rate of 6m/s and CO2 flow of 5 bar. Effect of temperature, chloride ion concentration, and inhibitor ratio has been studied. Accordingly, removal mass, surface layer mass and the corrosion rate were evaluated at different conditions. Fe2+ concentrations and pH values were also evaluated. The corrosion degree of the tested samples is assessed by polarizing microscope at different magnification values. Results showed that high corrosion rates affected by high temperatures and high chloride concentrations, have been particularly reduced by addition of Dodisclae V3962 inhibitor. Formation of a fixed surface layer mass might be the reason for the high corrosion inhibition. [DOI: 10.22401/JUNS.20.3.08]
在2l玻璃高压灭菌器中研究了天然气生产条件下钢环(钢管)的腐蚀。钢管轴向构造,腔径比为3×1 mm,在玻璃高压灭菌器中以6m/s的流速和5bar的CO2流量暴露于CO2饱和氯化物溶液中4天。研究了温度、氯离子浓度、缓蚀剂配比等因素的影响。在此基础上,对不同条件下的去除质量、表层质量和腐蚀速率进行了评价。并对Fe2+浓度和pH值进行了评价。利用偏光显微镜在不同倍率下对试样的腐蚀程度进行了评价。结果表明,加入Dodisclae V3962缓蚀剂后,受高温和高氯化物浓度影响的高腐蚀速率明显降低。形成固定的表面层质量可能是高缓蚀性的原因。(DOI: 10.22401 / JUNS.20.3.08)
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引用次数: 0
An Epidemiology Study of Some Protozoan Parasitic Diseases in Iraq from 2011 Till 2015 2011 - 2015年伊拉克部分原生动物寄生虫病流行病学研究
Pub Date : 2017-09-01 DOI: 10.22401/JUNS.20.3.17
Israa S. Mosa, Sinan Ghazi Mahdi, Muthana Ibrahim Abdul-Karim, Adnan Khistawi, E. Saheb, Baghdad-Iraq Helmanthology Units in Baghdad
The parasitic diseases including leishmaniasis, toxoplasmosis and malaria are globally wide spread with potentially harmful consequences if it does not treat. Leishmaniasis is caused by the protozoan parasites Leishmania. Many Leishmania spp. causes skin ulcers and nodules. Other species damage the internal organs. Toxoplasmosis is caused by the protozoan parasites Toxoplasma gondii. Malaria is one of tropical and semi-tropical parasitic diseases caused by the protozoan parasites Plasmodium. This study assesses the epidemiology of the leishmaniasis, toxoplasmosis and malaria infection for the period from 2011 till 2015 in Iraq. From 2011 till 2015, 3611 patients with visceral leishmaniasis, 21473 patients with cutaneous leishmaniasis, 4365 patients infected with toxoplasmosis, 31 patients with malarial infections were recorded in Iraq. The parasitic diseases including leishmaniasis and toxoplasmosis nowadays have a wider geographical distribution in Iraq. This increase is mainly attributed to the environmental conditions, great migration, urbanization that occurs during the study period. In contrast, according to this study malaria has been greatly reduced in Iraq in the last years and this could be due to the use of protective clothing, insecticides, insect repellents and bed nets.
包括利什曼病、弓形虫病和疟疾在内的寄生虫病在全球广泛传播,如果不加以治疗,可能造成有害后果。利什曼病是由原虫寄生虫利什曼原虫引起的。许多利什曼原虫引起皮肤溃疡和结节。其他种类会损害内部器官。弓形虫病是由原生动物寄生虫弓形虫引起的。疟疾是由原虫疟原虫引起的热带和半热带寄生虫病之一。本研究评估了2011 - 2015年伊拉克利什曼病、弓形虫病和疟疾感染的流行病学。2011 - 2015年,伊拉克共报告内脏利什曼病3611例,皮肤利什曼病21473例,弓形虫病4365例,疟疾感染31例。目前,包括利什曼病和弓形虫病在内的寄生虫病在伊拉克有更广泛的地理分布。这一增长主要归因于研究期间发生的环境条件、大规模移民和城市化。相比之下,根据这项研究,伊拉克的疟疾在过去几年中大大减少,这可能是由于使用了防护服、杀虫剂、驱虫剂和蚊帐。
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引用次数: 3
Weak Soft Separation Axioms and Weak Soft (1,2)*-D ~ -Separation Axioms in Soft Bitopological Spaces 软双拓扑空间中的弱软分离公理和弱软(1,2)*-D ~ -分离公理
Pub Date : 2017-09-01 DOI: 10.22401/JUNS.20.3.20
Sabiha I. Mahmood
Abstract: In this article we introduce and study new types of soft sets in soft bitopological spaces, namely, soft (1,2)*-difference sets and soft (1,2)*-b-difference sets by using the notion of soft -open sets and soft (1,2)*-b-open sets respectively. Furthermore we use these soft sets to define and study new types of soft separation axioms, namely, soft (1,2)*- -spaces and soft (1,2)*-b- -spaces for which are weaker than soft (1,2)*- -spaces and soft (1,2)*-b- -spaces for respectively. The basic properties and characteristics each of soft (1,2)*-b- -spaces, soft (1,2)*- -spaces and soft (1,2)*-b- -spaces for also have been studied.
摘要:本文分别利用软开集和软(1,2)*-b开集的概念,引入和研究了软双拓扑空间中的软集,即软(1,2)*-差分集和软(1,2)*-b-差分集。利用这些软集进一步定义和研究了新的软分离公理类型,即分别弱于软(1,2)*-空间和软(1,2)*-b-空间的软(1,2)*-空间和软(1,2)*-b-空间。研究了软(1,2)*-b-空间、软(1,2)*-空间和软(1,2)*-b-空间的基本性质和特征。
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引用次数: 2
Evaluation the Level of Dieldrin and Heptachlor in Iraqi People 伊拉克人狄氏剂和七氯胺水平评价
Pub Date : 2017-09-01 DOI: 10.22401/JUNS.20.3.05
Alaa H. Jawad
Organochlorine pesticides for whatever length of time that been extensively utilized as a part of farming and in public health as highly effective pest control agents.They are lipophelic and have drawn out a half-existences of years to decades; thus, they amass in human fat tessues and a can bring about endless poisonous quality after long term exposure.To detect and measure the concentrations of organochlorine pesticides, (Dieldrin and Heptachlor) in serum and fatty tissue samples and studies their correlation with lipids in order to reveal the need. for human monitoring.The study was conducted on 50 samples of blood and fatty tissues to determine their lipid concentrations and detect metabolites of organochlorine pesticides and asses their1correlations using HPLC.The study observed that there were raised serum concentrations of lipids which were positively corresponded with lifted serum convergences of Dieldrin and Heptachlor pesticides.Mean serum concentrations of triglycerides was inside “normal” range while mean serum of aggregate cholesterol was hoisted above ordinary range. Rate of grouping of lipid to serum Dieldrin was higher than that of Heptachlor in living subjects.
有机氯农药作为农业和公共卫生的一部分作为非常有效的害虫控制剂被广泛使用,无论使用时间长短。它们是亲脂性的,寿命长达几年到几十年;因此,它们在人体脂肪组织中聚集,长期暴露后会带来无尽的毒性。检测和测量血清和脂肪组织样品中有机氯农药(狄氏剂和七氯)的浓度,并研究其与脂质的相关性,以揭示其必要性。用于人类监控。本研究对50份血液和脂肪组织样本进行了脂质浓度测定和有机氯农药代谢物检测,并利用高效液相色谱法评估了它们之间的相关性。研究发现,血清脂质浓度升高与狄氏剂和七氯农药的血清收敛度升高呈正相关。平均血清甘油三酯浓度在“正常”范围内,而平均血清总胆固醇高于正常范围。血脂对血清狄氏剂的分组率高于七氯。
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引用次数: 0
تقيس األشعة فوق البنفسجية في كواشف األثر النووي العضوية UV-Visible مطيافية باستخدام CR-39 , CN- LR-115 85 在vvvibille光谱仪中测出的紫外线光度,分光分离式分光仪,CN- lr - 85 115
Pub Date : 2017-09-01 DOI: 10.22401/JNUS.17.3.31
فراس عبدالكريم محمد علي, قسم الفيزياء، كلية العلوم – جامعة الموصل., حسين علي الجبوري, احمد خلف محميد, قسم الفيزياء ، كلية العلوم ، جامعة النهرين, قسم الفيزياء، كلية العلوم – جامعة الموصل.
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引用次数: 0
期刊
Journal of Al-Nahrain University-Science
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