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GENETIC PROFILING AND DIVERSITY OF SOME PROMISING EFFICIENT RHIZOBIAL ISOLATES ON FABA BEAN PLANTS 蚕豆高效根瘤菌的遗传图谱及多样性研究
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175279
Doha M. Fathy, A. Eldomiaty, H. I. A. El-Fattah, E. Mahgoub, A. Hassanin
A group of twenty faba bean rhizobial isolates was collected from two Egyptian Governorates (Dakahlia and Damitta). The isolates were further morphologically and physiologically characterized to check their growth and symbiotic performance on faba bean plants. According to remarkable lab and pots tests, five rhizobial isolates (Rh 32, Rh 6-A, Rh 3-4, Rh RL3, and Rh 8-A) were selected and subjected to further biochemical and molecular characterizations. Genetic profiling of the five promising rhizobial isolates was conducted using six ISSR-primers. Amplification of bacterial genomic DNA produced a total of 37 genomic loci, 54% of them were polymorphic and 46% were monomorphic. The rate of polymorphism ranged between 25% to 80% with an average of 54%. Clustering pattern analysis of morphological and physiological data grouped the twenty rhizobial isolates in five clusters and the five selected rhizobial isolates were falling close to each other. Clustering analysis of ISSR data grouped the the five rhizobial isolates in four clusters. Analysis based on ISSR data revealed that the lowest genetic distance was 2.00 between Rh 6-A and Rh 3-4 isolates, while the highest genetic distance of 3.61 was between Rh 32 and each of Rh 6-A, Rh 3-4, and Rh RL3 isolates. The greatest similarity measurement was 0.931 between Rh 6-A and Rh 3-4 isolates; while the lowest similarity was 0.745 between Rh 32 and Rh 3-4 isolates. It can concluded that clustering pattern analysis based on molecular data could be used in facilitating the selection of rhizobial isolates that will be promising as a source of genes for biological nitrogen fixation and plant growth-promotion.
从埃及两个省(Dakahlia和Damitta)收集了一组20株蚕豆根瘤菌分离株。进一步对分离菌株进行形态和生理表征,考察其在蚕豆植株上的生长和共生性能。根据实验室和盆栽试验,选择了5株根瘤菌(Rh 32、Rh 6-A、Rh 3-4、Rh RL3和Rh 8-A),并进行了进一步的生化和分子表征。利用6条issr引物对5株有潜力的根瘤菌进行了遗传图谱分析。细菌基因组DNA扩增共产生37个基因组位点,其中54%为多态性,46%为单态。多态性率在25% ~ 80%之间,平均为54%。形态学和生理数据的聚类分析将20株根瘤菌分在5个聚类中,5个被选根瘤菌相互靠近。ISSR数据聚类分析将5株根瘤菌分在4个聚类中。ISSR分析显示,Rh 6-A和Rh 3-4分离株的遗传距离最低为2.00,Rh 32与Rh 6-A、Rh 3-4和Rh RL3分离株的遗传距离最高为3.61。Rh - 6-A与Rh - 3-4菌株的相似性测量值最高,为0.931;Rh 32与Rh 3-4的相似性最低,为0.745。结果表明,基于分子数据的聚类模式分析可用于根瘤菌分离株的筛选,这些分离株有望作为生物固氮和促进植物生长的基因来源。
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引用次数: 1
أثر تطبيق نظم الري الحديثة على محصول القمح في محافظة الشرقية 采用现代灌溉系统对东部省小麦收成的影响
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175506
أروى نور الدين, محمد جابر عامر, أنور لبن
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引用次数: 0
معــــوقــــــات اســــتخــدام الــــزراع لــوســـــائـل الاتصـــال الإلکتـــروني فـي الإرشــــاد الزراعـــى 在农业指导方面使用农业通讯的障碍
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175505
سعدلي سليم سعدلي سليم, ماجدة عبد الله عبد العال, طه على الفيشاوى, محمد الخولى
استهدف البحث بصفة رئيسية دراسة معوقات استخدام الزراع لوسائل الاتصال الإلکتروني في الإرشاد الزراعى، ولتحقيق هذا الهدف تم صياغة الأهداف البحثية الفرعية التالية: التعرف على بعض الخصائص المميزة  للمبحوثين والتعرف على الأجهزة التي يستخدمها المبحوثين في الحصول على معلوماتهم، والتعرف على المواقع التي يستخدمها  المبحوثين للحصول على المعلومات، وتحديد درجة استفادة المبحوثين من الاتصال الإلکتروني في الإرشاد الزراعي والعوامل المرتبطة بها، والتعرف على المعوقات التي تواجه المبحوثين عند استخدام وسائل الاتصال الإلکترونية ومقترحاتهم للحد منها، وأجرى هذا البحث في محافظة الشرقية وبلغت عينة البحث 370 مبحوثا من المستخدمين لوسائل الاتصال الالکترونية، وأوضح البحث أن حوالى (10,3%) من إجمالي المبحوثين يستفيدون من وسائل الاتصال الإلکتروني بدرجة ضعيفة، بينما(32,2%) من المبحوثين درجة استفادتهم متوسطة، في حين(66,5%) أى ما يقارب من ثلثى العينة يستفيدون بدرجة کبيرة،  کما أظهرت النتائج وجود علاقة ارتباطية معنوية بين درجة  استفادة المبحوثين من استخدام وسائل الاتصال الإلکترونى والمتغيرات المستقلة التالية: (تعليم المبحوث، عدد سنوات الخبرة في العمل الزراعى، عدد أفراد الأسرة العاملين بالزراعة، حيازة الأرض الزراعية، ودرجة المشارکة الاجتماعية غير الرسمية، والدرجة القيادية، ودرجة الاتجاه نحو المستحدثات الزراعية، ودرجة الاستعداد للتغيير، الاتصال بالإرشاد الزراعي، اتجاهات الزراع نحو المرشد الزراعي بالقرية)، وبينت النتائج أن درجة وجود المعوقات التى  تواجه المبحوثين بدرجة ضعيفة بلغت حوالى (0,8%)، بينما درجة وجودها بدرجة متوسطة بلغت حوالى 24,1%، فى حين بلغت درجة وجودها بدرجة کبيرة حوالى 75,1%، وأوضحت النتائج وجود علاقة ارتباطية معنويه موجبة عند مستوى معنوية (0,01) بين درجة وجود المعوقات التي تواجه المبحوثين عند استخدام وسائل الاتصال الإلکترونى وبين المتغيرات المستقلة التالية (تعليم المبحوث، عدد سنوات الخبرة في العمل الزراعي، عدد الأفراد العاملين بالزراعة، الاتجاه نحو المستحدثات الزراعية، درجة الاستعداد للتغيير)، ووجود علاقة موجبة وطردية عند مستوى معنوية (0,05) بين وجود المعوقات التى تواجه  المبحوثين عند استخدام وسائل الاتصال الإلکتروني وبين المشارکة الاجتماعية الرسمية، الاتصال بالإرشاد الزراعى، اتجاهات الزراع نحو المرشد الزراعي بالقرية.
这项研究的主要目的是研究农民在农业推广中使用电子通讯手段的障碍,为此,制定了以下次级研究目标:确定研究人员的一些特征,确定研究人员获取信息时使用的设备,确定研究人员在农业推广中使用电子通信的程度和相关因素,并确定研究人员在使用电子通信手段和限制使用电子通信手段时所面临的障碍和建议,这项研究是在东部省进行的,有370名研究人员的样本显示,在所有研究人员中,有10.3%的人的电子通讯水平较低,32.2%的人的使用程度为中等,66.5%的人(约三分之二的人)使用了凯啤酒,结果显示,研究人员使用电子通讯的程度与下列独立变量之间存在着精神联系:研究人员教育、农业工作经验年数、农业家庭成员人数、农地保有权、非正式社会参与程度、领导程度、向农业发展方向发展的程度、变化的准备程度、与农业推广的联系、农民向农村农业推广的趋势),研究结果表明,研究人员的残疾程度大约低(0.8%),中等程度约为24%,凯比尔约为75.1%。结果显示,在研究人员在使用电子通信时所面临的障碍的程度与下列独立变量(研究人员教育、农业工作经验年数、农业工人人数、农业创新趋势、变化准备程度)之间存在一种在道德水平(0.05)之间存在的情感联系在道德水平(0.05)上,研究人员在使用电子通信时所面临的障碍与官方社会参与和推广联系之间存在着一种正的联系。农民趋势,农村农业指南。
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引用次数: 0
EVALUATION OF AN AERATION SYSTEM FOR FISH CULTURED IN CLOSED SYSTEMS 密闭养殖鱼类曝气系统的评价
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175262
Amr I. Said, M. Shazly, A. Zedan, A. Hassan
This study was Carried out at Central Laboratory for Aquaculture Research in (2017), to determine the best levels of oxygen supply on growth performance and oxidation status of Nile tilapia (Oreochromis nilollcus), Grey mullet (Mugil cephalus) and Common carp (Cyprinus carpio) fingerlings stocked at (85%, 10% and 5%), respectively, total of fingerlings with initial weight of weighting (10±0.30) g/fish were reared in tanks i.e., supplied with normal water, air pumping and filtration system applied throughout the experimental period, with 3 replicates to evaluate the effect of perforated pipe location i.e., 0 and 100 cm from the bottom of tanks, distance between holes such as : 10cm, air pressure levels i.e., 0.2 and 0.4 bar on water quality, growth rate of fish and net profit. The obtained results revealed that the perforated pipe located at 100 cm with distance between holes such as: 10 cm and air pressure (P) of 0.4 bar gave the highest average of water quality like pH (7.72±.30 and 8.18±2.48 mg/l), dissolved oxygen (7.42±.29 and 8.38±2.54 mg/l), water temp (27C ±.81), ammonia (0.29±.01 and 0.28±.08 mg/l), total hardness (171.4±6.85 and 170.9±51.97), total alkalinity (165.3 ± 6.61 and 180.7±54.95 mg/l), nitrate (0.01±.0004 and 0.01±.003), nitrite (0.11±.004 and 0.12±.03) for air pressure levels (0.2 and 0.4) bar respectively. Compared with control result which were 6.7±.30, 4.45±2.54, 0.32±01, 25.0±2.16, 340±2.71, 370±12.7, 0.12±008 and 0.40±0.001 for pH, dissolved oxygen, water temp, ammonia, total hardness, total alkalinity, nitrate and nitrite. The highest average of growth rate values; final body weight (74.9±22.77, 77.4±1.16 and71.9±3.59 g), final body length (19.2±5.83, 18.2±.27 and 16.4±0.32 cm), final body weight gain (5±0.20, 4.4±0.06and 4.6±0.09g), final condition factor (1.36±0.05, 1.38±0.23and 2.61±0.26g/cm 3 ) and final feed conversion ratio (1.56±0.47, 1.62±0.02 and1.68 ± 0.03 g/fish), Final SGR were (1.93±0.58, 1.68±0.02 and 2.0 ±0.04%) and final survival rate (%) were (91.8±2.9 , 88.9±2.6 and 90.2±1.2) for Nile tilapia (Oreochromis nilollcus), Grey mullet (Mugil cephalus) and Common carp (Cyprinus carpio) and distances between holes of 10 cm and pressure levels of 0.4 bar Respectively. Finally, it could be concluded that, under the similar conditions: The Air pressure (P) level of 0.4 bar, perforated pipe location of 100 cm and distance between holes 10 cm were the best parameters where it gave Significantly high water quality, growth performance (P<0.0001) and survival of fish (P<0.0001) was observed in aerated tanks as compared with the non-aerated tanks.
本研究于2017年1月在水产养殖研究中心实验室开展,旨在确定尼罗罗非鱼(Oreochromis nilollcus)、灰鲻鱼(Mugil cephalus)和鲤鱼(Cyprinus carpio)鱼种的最佳供氧水平,分别为(85%、10%和5%),初始体重为(10±0.30)g/鱼的鱼种在水箱中饲养,即在正常水的条件下饲养。在整个试验期间,采用抽气和过滤系统,共3次重复,以评估孔管位置(距罐底0和100 cm)、孔距(10cm)、气压(0.2和0.4 bar)对水质、鱼的生长速度和净利润的影响。结果表明,当孔距为10cm、孔距为100cm、气压为0.4 bar时,pH值(7.72±7.72)等水质平均值最高。30和8.18±2.48 mg/l),溶解氧(7.42±2.48 mg/l)。29和8.38±2.54 mg/l),水温(27℃±0.81),氨(0.29±2.54)。0.01和0.28±。总硬度(171.4±6.85和170.9±51.97),总碱度(165.3±6.61和180.7±54.95 mg/l),硝酸盐(0.01±54.95 mg/l)。0004和0.01±0.003),亚硝酸盐(0.11±0.003)。空气压力水平(0.2和0.4)bar分别为004和0.12±0.03)。对照结果为6.7±。pH、溶解氧、水温、氨、总硬度、总碱度、硝酸盐和亚硝酸盐分别为30、4.45±2.54、0.32±01、25.0±2.16、340±2.71、370±12.7、0.12±008和0.40±0.001。生长速率值的最高平均值;最终体重分别为74.9±22.77、77.4±1.16和71.9±3.59 g,最终体长分别为19.2±5.83、18.2±3.59 g。尼罗罗非鱼(Oreochromis nilollcus)的终增重(5±0.20、4.4±0.06和4.6±0.09g)、终条件因子(1.36±0.05、1.38±0.23和2.61±0.26g/ cm3)和终饲料系数(1.56±0.47、1.62±0.02和1.68±0.03 g/鱼)、终SGR(1.93±0.58、1.68±0.02和2.0±0.04%)和终存活率(%)分别为(91.8±2.9、88.9±2.6和90.2±1.2)。灰鲻鱼(Mugil cephalus)和鲤鱼(Cyprinus carpio),孔间距离为10厘米,压力水平为0.4巴。综上所示,在相同条件下,曝气池的最佳参数为气压(P)水平为0.4 bar,穿孔管位置为100 cm,孔距为10 cm,与不曝气池相比,曝气池的水质、生长性能(P<0.0001)和鱼的存活率(P<0.0001)均显著高于不曝气池。
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引用次数: 1
القــدرة التنـافسـية للفـراولـة المـصـريـة فـي أهـم الأسـواق العـالمـيـة 埃及在全球最重要市场的竞争力
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175507
محمود جمال أحمد خلف, خيري إبراهيم, مکادى سليمان
تواجه صادرات مصر بصفة عامة وصادرات محاصيل الخضر بصفة خاصة الکثير من التحديات التي تؤدى الى تذبذبها بين الزيادة والنقصان من عام لآخر، بالإضافة الى زيادة التنافسية بين الدول المختلفة. وقد واجهت صادرات الفراولة المصرية من تناقص القوه  التصديرية لها وکذلک الأنصبة السوقية في السنوات الأخيرة ، لذا يهدف البحث إلى دراسة المرکز التنافسي لمحصول الفراولة خلال الفترة (2003-2018) في أهم الأسواق التصديرية، وتحليل هيکل صادرات مصر من محصول الفراولة، حيث تبين من خلال النتائج أن مصر تتمتع بميزة نسبية عالية لصادراتها من الفراولة  ، وبدراسة التوزيع الجغرافي للصادرات المصرية من الفراولة تبين أن السعودية احتلت المرکز الأول من حيث کمية وقيمة الصادرات، يليها کل من بلجيکا وألمانيا، وبدراسة النصيب السوقي تبين أن مصر تحتل المرتبة السادسةبين دول العالم ،حيث بلغ متوسط کمية الصادرات خلال قترة الدراسة حوالي 30,84 ألف طن وهي تمثل حوالي 3,37%من متوسط صادرات العالم من الفراولة خلال متوسط فترة الدراسة، وقد تبين من تقدير مؤشر اختراق السوق للفراولة المصرية بتلک الأسواق إلى أن نسب ما تستورده إلي استهلاکها صغير جدا مما يتيح إماکينة زيادة التصدير إلي تلک الدول مع الألتزام بمتطلباتها ودراسة أذواق المستهلک بها، کما اتضح من دراسة السعر النسبي قوة المرکز التنافسي لصادرات مصر من الفراولة في مواجهه جميع الدول المنافسة وخاصة هولندا، الولايات المتحدة الأمريکية،اسبانيا نظرا لارتفاع قيمة السعر النسبي عن الواحد الصحيح ، مما يتيح لمصر فرصا أکبر في المنافسة والتصدير. ومن خلال دراسة مؤشر القدرة على الوفاء بمتطلبات التصدير تبين من النتائج أن مصر تحتل المرکز الثالث من حيث عدم الاستقرار النسبي في الانتاج المحلي لمحصول الفراولة يليها کل من إسبانيا ،المکسيک.
总体而言,埃及的出口,尤其是蔬菜作物的出口,面临着许多挑战,除了增加不同国家之间的竞争力外,这些挑战还导致其每年的增减波动。近年来,埃及草莓出口面临着出口能力和市场份额的下降,因此,这项研究旨在研究2003-2018年期间草莓作物在最重要出口市场中的竞争地位,并分析埃及草莓作物出口结构,结果表明,埃及草莓出口具有很高的相对优势,对埃及草莓出口地理分布的研究表明,沙特阿拉伯在数量上排名第一。出口价值,其次是比利时和德国,对市场份额的研究表明,埃及在世界国家中排名第六,研究期间的平均出口量约为3.84万吨,约占世界平均出口量的3.37%。从研究期间的平均草莓来看,对埃及草莓市场渗透指数的估计表明,其进口量与消费量的比率非常小,这使得能够在遵守其要求的同时增加对这些国家的出口。对消费者口味的研究表明,相对价格的研究表明,埃及草莓出口的竞争地位在所有竞争国家面前的实力,尤其是荷兰、美国和西班牙,因为相对价格的价值高于一个是正确的,这为埃及提供了更大的竞争和出口机会。通过对满足出口需求能力指数的研究,结果表明,埃及草莓作物国内生产相对不稳定排名第三,其次是西班牙和墨西哥。
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引用次数: 0
أثــر الســـعة الإنتاجيـــة فــي مــــزارع البــــداري عـلـــى الربحية بمحـافظــة الشـــرقيــــة (دراســــة حالة في مرکــز بلبيس) 巴利什农场的生产能力对东部省利润的影响
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175289
إیناس على, علي كامل إبراهيم, أسامة عويضة, أحمد فوزى حامد
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引用次数: 0
ENHANCING THE PYROLYTIC GAS PRODUCTION GENERATED BY SLOW PYROLYSIS USING A FIXED-BED PROLYZER 利用固定床催化装置提高慢速热解产气率
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175267
Ahmed Zahra, M. El-Wahab, M. Tawfik
The biomass slow pyrolysis using the fixed-bed pyrolyzer is a simple and inexpensive technique that can be used in small farms comparing to the complex and expensive technology of the fast pyrolysis. However, slow pyrolysis primary produces bio-char and lower yields of bio-oil and pyrolytic gas. Thus, there is urgent need to enhance pyrolytic gas production under the slow pyrolysis conditions on the account of bio-char due to the advantage of using the gas in wide applications in farm. Hence, this work aims to fabricate and operate a small-scale fixed bed pyrolyzer to enhance the pyrolytic gas productivity from the ground pieces of Mango trees Pruning Logs (MPLs) under slow pyrolysis conditions (heating rate of 0.01-1 oC/s, vapor residence time of 4 min.) and full absence of oxygen. The pyrolytic gas production was investigated under different final pyrolysis temperatures of 300,350 and 400°C, particle length ranges of 1-5, 10-15 and 20-25 mm. The obtained results revealed that, the increase of pyrolysis temperature from 300-400°C and decrease of the ground MPL length from 20-25 to 1-5 mm was accompained with explicit increment in the pyrolytic gas concentration, gas yield, higher heating value of pyrolytic gas (HHVg) and energy conversion efficiency by about 34.28%, 61.15%, 12.68% and 66.10%, respectively along to high concentrations of the combustible gases )e.i. H2, CH4). Additionally, the optimal cost per energy unit of pyrolytic gas (0.085 EGP/MJ or 0.005 $/MJ) is lower than cost per energy unit of the natural gas and LPG by about 11.45% and 75.07%, respectively.
与复杂而昂贵的快速热解技术相比,使用固定床热解器的生物质慢速热解是一种简单而廉价的技术,可用于小型农场。然而,慢速热解初级产物产生生物炭,生物油和热解气体的产率较低。因此,由于生物炭具有在农场广泛应用的优势,迫切需要在缓慢热解条件下提高热解气体的产量。因此,本工作旨在制造和运行一个小型固定床热解器,以在缓慢热解条件下(加热速率为0.01-1℃/s,蒸汽停留时间为4分钟)和完全缺氧的情况下,提高芒果树修剪原木碎片的热解气体生产率。研究了在不同的最终热解温度(300350和400°C,颗粒长度范围为1-5、10-15和20-25mm)下热解气体的产生。结果表明,热解温度从300-400°C升高,地面MPL长度从20-25mm减小,热解气体浓度、产气量、,较高的热解气体热值(HHVg)和能量转换效率分别提高了约34.28%、61.15%、12.68%和66.10%,以及高浓度的可燃气体(例如H2、CH4)。此外,热解气体的每能量单位的最佳成本(0.085 EGP/MJ或0.005$/MJ)分别比天然气和液化石油气的每能量单元的成本低约11.45%和75.07%。
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引用次数: 1
STUDY ON SOME DIFFERENT OPERATING PARAMETERS AFFECTING THE PERFORMANCE OF COMPOST TURNING MACHINES 几种不同操作参数对堆肥翻转机性能影响的研究
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175271
D. Sayed, M. M. Morad, Mohamed A. Ali, Hend A M El-Maghawry
Experiments were carried out to evaluate the performance of two compost turning machines for producing organic fertilizer by recycling agricultural wastes. To fulfill the objective of this research work, some operating parameters affecting the performance of the two compost turning machines were taken into consideration: Three different types of field crop residues of  rice straw, cotton stalks and corn stalks, four different machine forward speeds of 1.0, 1.2, 1.4 and 1.6 km/h, four different turning drum speeds of 150, 200, 250, and 300 rpm and four turning times of 1, 2, 3 and 4 times/month were considered. Evaluation of the compost turning machines was carried out taking into consideration compost density, period to compost maturity, machine productivity, energy requirements and final compost quality. The obtained data revealed that the compost density, the period to compost maturity, the machine productivity, energy requirements and final compost quality were in the optimum range with the use of the self-propelled compost turning machine under conditions of 1.4 km/h forward speed, 250 rpm turning drum speed and three turning times/month. Results also revealed that corn stalks required the least period for maturity of 8 weeks, followed by rice straw of 12 weeks while cotton stalks required the longest period for maturity of16 weeks.
通过试验,对两种利用农业废弃物生产有机肥的堆肥机进行了性能评价。为实现本研究的目的,研究了影响两种堆肥翻车机性能的操作参数:考虑了水稻秸秆、棉花秸秆和玉米秸秆3种不同类型的田间作物秸秆,1.0、1.2、1.4和1.6 km/h 4种不同的机器前进速度,150、200、250和300 rpm 4种不同的翻车滚筒转速,1、2、3和4次/月的翻车次数。综合考虑堆肥密度、堆肥成熟期、机器生产率、能量需求和最终堆肥质量等因素,对堆肥机进行了评价。结果表明,在前进速度1.4 km/h、转鼓速度250 rpm、每月翻3次的条件下,自行式翻车堆肥机的堆肥密度、堆肥成熟周期、机器生产率、能量需求和最终堆肥质量均在最佳范围内。玉米秸秆成熟期最短,为8周,其次是水稻秸秆,为12周,棉花秸秆成熟期最长,为16周。
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引用次数: 1
THE FITNESS OF A GEM STRAIN OF Pseudomonas aeruginosaBACTERIA COMPARING WITH ITS PARENT STRAINS UNDER LABORATORY CONDITIONS 铜绿假单胞菌GEM菌株与其亲本菌株在实验室条件下的适应性比较
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175285
Salah Maaty, A. Hassan, M. Aminand, M. Elashtokhy
The fitness of a genetically engineered microorganism strain that constructed as transconjugant by conjugation mechanism of horizontal gene transfer in Pseudomonas aeruginosa bacteria comparing with its parent strains has been assessed. Ecological fitness expresses interaction of an organism with its environment. This consider as a good indicator for the assessment of Genetically Engineered Microorganism (GEM). When released into the environment this assay was carried out under laboratory condition to investigate the effects of environmental condition on the fitness. These laboratory condition included the effect of different cations (Na + , Ca +2 , and Fe +3 ), different incubation temperatures, pH values and different ratios of the two parents. Increasing of mono, and di cations (Na + , Ca +2 ) concentrations revealed an increasing in the fitness of parents and transconjugant (GEM( strain up to the maximum concentration (105 mM) with Na + and (85 mM) with Ca +2 and then began to decrease. In the three strains temperature plays an important role in gene transfer rate and fitness, There was a direct relationship between the increasing in temperature until it reaches the optimum temperature and the rate of gene transfer. Also, the highest rate of gene transfer via conjugation appeared when the ratio between the donor and the recipient was 3:1. The best results for bacterial fitness for transconjugant strain and parents appeared at pH 9, which means increased fitness for both of them. These results show that the transconjugant strain can be have and survive well as its parent strain and the effects of some ecological factor are similar in the three strains.
通过对铜绿假单胞菌水平基因转移偶联机制构建的转基因微生物菌株与其亲本菌株的适应性进行了评估。生态适应性表达了一个有机体与其环境的相互作用。这被认为是评估基因工程微生物(GEM)的良好指标。当释放到环境中时,在实验室条件下进行该测定,以调查环境条件对适应性的影响。这些实验室条件包括不同阳离子(Na+、Ca+2和Fe+3)、不同培养温度、pH值和两个亲本的不同比例的影响。单阳离子和二阳离子(Na+,Ca+2)浓度的增加表明亲本和转偶联物(GEM)的适应度增加,Na+和Ca+2分别达到最大浓度(105mM)和(85mM),然后开始降低。在这三个菌株中,温度对基因转移速率和适合度起着重要作用。温度升高直到达到最佳温度与基因转移速率之间存在直接关系。此外,当供体和受体之间的比例为3:1时,通过接合的基因转移率最高。跨偶联菌株和亲本的细菌适应度的最佳结果出现在pH 9时,这意味着它们的适应度都增加了。这些结果表明,转基因菌株可以与其亲本菌株一样具有并存活良好,并且某些生态因素的影响在这三个菌株中是相似的。
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引用次数: 0
PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ONION(Allium cepa L.) EXTRACTS 洋葱提取物的植物化学筛选及其抗氧化活性
Pub Date : 2021-03-01 DOI: 10.21608/ZJAR.2021.175288
M. El-Saied, S. El‐Saadany, H. Hefnawy, A. El-Sayed
Phytochemical screening and antioxidant activities in different solvent extracts of onion was carried out. The extracts were subjected to various chemical Test for phytochemical constituents. Total phenolic contents were evaluated using Folin Ciocalteu method and their antioxidant activity was assayed through «in vitro» radical scavenging activity using DPPH· assay, FRAP and ABTS. The phytochemical screening of this study indicated the presence of steroids, flavonoids, alkaloids, saponnins, and catechic tannins. The average total polyphenol content of hydroethanolic extracts was significantly (P<0.05) higher than in the hexane, ethyl acetate and dichloromethane extracts. The order of effectiveness (IC50) of the plant extracts the potent inhibitors was hydroethanolic extract, followed by Dichloromethane, ethyl acetate while the least was the hexane extract, When using (DPPH, ABTS and FRAP). This shows that onion organic solvent extracts especially the hydroethanolic extracts may be a potent source of natural antioxidant and can be used in the management of diseases associated with oxidative stress is justified
对洋葱不同溶剂提取物进行了植物化学筛选和抗氧化活性研究。对提取物进行植物化学成分的各种化学测试。用Folin-Ciocalteu法测定总酚含量,并用DPPH·assay、FRAP和ABTS通过“体外”自由基清除活性测定其抗氧化活性。本研究的植物化学筛选表明存在类固醇、类黄酮、生物碱、皂苷和儿茶素单宁。水乙醇提取物的平均总多酚含量显著高于己烷、乙酸乙酯和二氯甲烷提取物(P<0.05)。在使用(DPPH、ABTS和FRAP)时,植物提取物的有效性顺序(IC50)是有效的抑制剂是水乙醇提取物,其次是二氯甲烷、乙酸乙酯,而己烷提取物最少。这表明洋葱有机溶剂提取物,特别是水乙醇提取物,可能是天然抗氧化剂的有效来源,可用于治疗与氧化应激相关的疾病
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引用次数: 1
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Journal of Agricultural Research
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