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Development of 5-fluorouracil/etoposide co-loaded electrospun nanofibrous scaffold for localized anti-melanoma therapy. 开发用于局部抗黑色素瘤治疗的 5-氟尿嘧啶/埃托泊苷共载电纺纳米纤维支架。
IF 3.1 4区 医学 Q2 BIOPHYSICS Pub Date : 2024-01-01 DOI: 10.1177/22808000241284439
Shirin Shojaei, Mahtab Doostan, Hamidreza Mohammadi Motlagh, Seyedeh Sara Esnaashari, Hassan Maleki

Nanofibrous scaffolds have emerged as promising candidates for localized drug delivery systems in the treatment of cutaneous cancers. In this study, we prepared an electrospun nanofibrous scaffold incorporating 5-fluorouracil (5-FU) and etoposide (ETP) for chemotherapy targeting melanoma cutaneous cancer. The scaffold was composed of polyvinyl alcohol (PVA) and chitosan (CS), prepared via the electrospinning process and loaded with the chemotherapeutic agents. We conducted relevant physicochemical characterizations, assessed cytotoxicity, and evaluated apoptosis against melanoma A375 cells. The prepared 5-FU/ETP co-loaded PVA/CS scaffold exhibited nanofibers (NFs) with an average diameter of 321 ± 61 nm, defect-free and homogenous morphology. FTIR spectroscopy confirmed successful incorporation of chemotherapeutics into the scaffold. Additionally, the scaffold demonstrated a hydrophilic surface, proper mechanical strength, high porosity, and efficient liquid absorption capacity. Notably, sustained and controlled drug release was observed from the nanofibrous scaffold. Furthermore, the scaffold significantly increased cytotoxicity (95%) and apoptosis (74%) in A375 melanoma cells. Consequently, the prepared 5-FU/ETP co-loaded PVA/CS nanofibrous scaffold holds promise as a valuable system for localized eradication of cutaneous melanoma tumors and mitigation of adverse drug reactions associated with chemotherapy.

纳米纤维支架已成为治疗皮肤癌的局部给药系统的理想候选材料。在这项研究中,我们制备了一种电纺纳米纤维支架,其中含有 5-氟尿嘧啶(5-FU)和依托泊苷(ETP),用于针对黑色素瘤皮肤癌的化疗。该支架由聚乙烯醇(PVA)和壳聚糖(CS)组成,通过电纺工艺制备,并添加了化疗药物。我们进行了相关的物理化学表征,评估了细胞毒性,并评价了黑色素瘤 A375 细胞的凋亡情况。制备的 5-FU/ETP 共负载 PVA/CS 支架呈现出平均直径为 321 ± 61 nm 的纳米纤维(NF),形态无缺陷且均匀。傅立叶变换红外光谱证实了化疗药物成功加入支架。此外,该支架还具有亲水性表面、适当的机械强度、高孔隙率和高效的液体吸收能力。值得注意的是,从纳米纤维支架中观察到了持续、可控的药物释放。此外,该支架还显著提高了 A375 黑色素瘤细胞的细胞毒性(95%)和凋亡率(74%)。因此,制备的 5-FU/ETP 共负载 PVA/CS 纳米纤维支架有望成为局部根治皮肤黑色素瘤肿瘤和减轻化疗相关药物不良反应的重要系统。
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引用次数: 0
Chitosan-based promising scaffolds for the construction of tailored nanosystems against osteoporosis: Current status and future prospects. 基于壳聚糖的有前景的支架可用于构建针对骨质疏松症的定制纳米系统:现状与前景。
IF 3.1 4区 医学 Q2 BIOPHYSICS Pub Date : 2024-01-01 DOI: 10.1177/22808000241266487
Ya-Ming Wang, Jiang-Tao Shen

Despite advancements in therapeutic techniques, restoring bone tissue after damage remains a challenging task. Tissue engineering or targeted drug delivery solutions aim to meet the pressing clinical demand for treatment alternatives by creating substitute materials that imitate the structural and biological characteristics of healthy tissue. Polymers derived from natural sources typically exhibit enhanced biological compatibility and bioactivity when compared to manufactured polymers. Chitosan is a unique polysaccharide derived from chitin through deacetylation, offering biodegradability, biocompatibility, and antibacterial activity. Its cationic charge sets it apart from other polymers, making it a valuable resource for various applications. Modifications such as thiolation, alkylation, acetylation, or hydrophilic group incorporation can enhance chitosan's swelling behavior, cross-linking, adhesion, permeation, controllable drug release, enzyme inhibition, and antioxidative properties. Chitosan scaffolds possess considerable potential for utilization in several biological applications. An intriguing application is its use in the areas of drug distribution and bone tissue engineering. Due to their excellent biocompatibility and lack of toxicity, they are an optimal material for this particular usage. This article provides a comprehensive analysis of osteoporosis, including its pathophysiology, current treatment options, the utilization of natural polymers in disease management, and the potential use of chitosan scaffolds for drug delivery systems aimed at treating the condition.

尽管治疗技术不断进步,但骨组织受损后的恢复仍是一项具有挑战性的任务。组织工程或靶向给药解决方案旨在通过制造能模仿健康组织结构和生物特性的替代材料,满足临床对替代治疗方法的迫切需求。与人造聚合物相比,从天然来源提取的聚合物通常具有更强的生物相容性和生物活性。壳聚糖是一种独特的多糖,由甲壳素通过脱乙酰化作用提取而成,具有生物降解性、生物相容性和抗菌活性。其阳离子电荷使其有别于其他聚合物,成为各种应用的宝贵资源。通过硫代、烷基化、乙酰化或加入亲水基团等改性措施,壳聚糖的溶胀行为、交联性、粘附性、渗透性、可控药物释放性、酶抑制性和抗氧化性都能得到增强。壳聚糖支架在多种生物应用中具有相当大的利用潜力。壳聚糖支架在药物分布和骨组织工程领域的应用就很引人关注。由于壳聚糖具有良好的生物相容性和无毒性,因此是这种特殊用途的最佳材料。本文全面分析了骨质疏松症,包括其病理生理学、当前的治疗方案、天然聚合物在疾病管理中的应用,以及壳聚糖支架在药物输送系统中的潜在用途,旨在治疗骨质疏松症。
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引用次数: 0
Physical and mechanical properties assessment of glass ionomer cements modified with TiO2 and Mg-doped hydroxyapatite nanoparticles. 用二氧化钛和掺镁羟基磷灰石纳米颗粒改性的玻璃离聚体水门汀的物理和机械性能评估
IF 3.1 4区 医学 Q2 BIOPHYSICS Pub Date : 2024-01-01 DOI: 10.1177/22808000241282184
Bojana Ramić, Milica Cvjetićanin, Branislav Bajkin, Milan Drobac, Marija Milanović, Dragan Rajnović, Veljko Krstonošić, Đorđe Veljović

High viscosity glass ionomer cements (GICs) are widely used in various clinical applications, being particularly effective in atraumatic restorative treatment (ART) due to the synergistic interaction between the material and the technique. However, the inadequate mechanical properties of GICs raise concerns regarding the predictability and longevity of these restorations in areas exposed to occlusal stress. Various modifications of the powder components have been proposed to improve the mechanical strength of GICs to withstand occlusal loading during mastication. In this in vitro study, we investigated whether the nanoparticles (NPs) added to commercially available GICs could fulfill this requirement, which would likely broaden the spectrum of their potential clinical applications. Two commercially available GIC powders (Fuji IX and Ketac Molar), modified by the addition of 5 wt.% TiO2, MgHAp100 or MgHAp1000 NPs, were incorporated into the corresponding liquid in an appropriate ratio, and the mixed cements were evaluated in terms of fracture toughness, flexural strength, Vickers microhardness and rheological tests and compared with the original material. Fuji IX containing 5 wt.% MgHAp100 NPs had lower flexural strength, while Ketac Molar with 5 wt.% TiO2 NPs showed increased fracture toughness. Vickers microhardness increased in Fuji IX following the addition of 5 wt.% TiO2 and MgHAp100 but decreased in Ketac Molar comprising 5 wt.% MgHAp100 (p < 0.05). Achieving a predictable bond between NPs and cement matrix, as well as ensuring a uniform distribution of the NPs within the cement, are critical prerequisites for enhancing the mechanical performance of the original cement.

高粘度玻璃离聚体水门汀(GIC)被广泛用于各种临床应用,由于材料和技术之间的协同作用,它在非创伤性修复治疗(ART)中尤其有效。然而,由于 GIC 的机械性能不足,人们担心这些修复体在咬合应力作用下的可预测性和使用寿命。为了提高 GIC 的机械强度以承受咀嚼时的咬合负荷,人们提出了对粉末成分进行各种改良的建议。在这项体外研究中,我们调查了添加到市售 GIC 中的纳米颗粒(NPs)是否能满足这一要求,这可能会扩大其潜在的临床应用范围。我们将两种市售的 GIC 粉末(Fuji IX 和 Ketac Molar)以适当的比例加入到相应的液体中,并通过添加 5 wt.% 的 TiO2、MgHAp100 或 MgHAp1000 NPs 对其进行改性,然后从断裂韧性、抗弯强度、维氏显微硬度和流变测试等方面对混合水门汀进行评估,并与原始材料进行比较。含有 5 wt.% MgHAp100 NPs 的 Fuji IX 的抗折强度较低,而含有 5 wt.% TiO2 NPs 的 Ketac Molar 的断裂韧性有所提高。添加 5 wt.% TiO2 和 MgHAp100 后,Fuji IX 的维氏硬度增加了,但含有 5 wt.% MgHAp100 的 Ketac Molar 的维氏硬度降低了(p<0.05)。
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引用次数: 0
Picoscopy Discoveries of the Binary Atomic Structure 双原子结构的皮镜发现
4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-06-30 DOI: 10.35745/afm2023v03.02.0001
Olexandr Kucherov, Andrey Mudryk
In this article, we present a discovery of the binary atomic structure. Through picoscopy experiments, it was revealed that electronic structure is divided into core and functional structures. Internal chemically neutral electrons form the core of an atom and are spherical in pink, while the outer functional electrons are elongated in green being chemically active. A spherical yellow layer separates these two parts. It significantly simplifies the Schrödinger equation and leads to a solution for all 118 chemical elements. As a result, the Kucherov-Mudryk formula w = n + ¾l was derived. That formula allowed for organizing the periodic table in ascending order of the whole energy where en electron first fills the level with the lowest energy, according to the Minimum Potential Energy general principle of nature.
在这篇文章中,我们提出了二元原子结构的一个发现。通过picoscopy实验,发现其电子结构分为核心结构和功能结构。内部的化学中性电子构成了原子的核心,呈粉红色球形,而外部的功能电子呈绿色,呈细长状,具有化学活性。一个球形的黄色层将这两部分隔开。它极大地简化了Schrödinger方程,并得出了所有118种化学元素的解。由此推导出Kucherov-Mudryk公式w = n +¾l。这个公式允许按照整个能量的升序来组织元素周期表,其中一个电子首先填满了能量最低的能级,根据自然的最小势能一般原理。
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引用次数: 0
Effect of Ga Concentration on the Output Performance of ZnO Piezoelectric Nanorods Nanogenerator Ga浓度对ZnO压电纳米棒输出性能的影响
4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-06-30 DOI: 10.35745/afm2023v03.02.0002
Tung-Lung Wu, Teen-Hang Meen, Yu-Chuan Chang
In this study, a self-assembled monolayer of octyltriethoxysilane was grown on ITO glass. Subsequently, a hydrothermal method was employed to grow low-density gallium (Ga)-doped zinc oxide (ZnO) nanorod structures. In this growth process, the undoped pure ZnO nanorods and ZnO nanorods doped with five different Ga concentrations were developed. After growing the nanorods, X-ray diffraction (XRD) analysis was conducted on both undoped pure ZnO and Ga-doped ZnO nanorods to observe the influence of Ga concentration on the crystalline structure of the ZnO nanorods. Additionally, scanning electron microscopy (SEM) was utilized to examine changes in the surface and cross-sectional growth of ZnO nanorods with varying Ga concentrations, thereby investigating the impact of Ga concentration on the growth of ZnO nanorods. Finally, a thin Pt film was sputtered onto the ZnO nanorod structures to assemble nanogenerators. Ultrasonic excitation was applied to develop these nanogenerators for electrical measurements, allowing us to explore the effects of metal doping on the nanorods’ electrical properties.
在本研究中,在ITO玻璃上生长了一层自组装的辛基三乙基氧基硅烷单层。随后,采用水热法生长低密度镓掺杂氧化锌纳米棒结构。在此生长过程中,制备了未掺杂的纯ZnO纳米棒和掺杂5种不同Ga浓度的ZnO纳米棒。生长纳米棒后,对未掺杂的纯ZnO和掺杂Ga的ZnO纳米棒进行x射线衍射(XRD)分析,观察Ga浓度对ZnO纳米棒晶体结构的影响。此外,利用扫描电子显微镜(SEM)研究了不同Ga浓度下ZnO纳米棒表面和截面生长的变化,从而研究了Ga浓度对ZnO纳米棒生长的影响。最后,在ZnO纳米棒结构上溅射一层薄薄的Pt薄膜来组装纳米发电机。超声波激发被应用于这些纳米发电机的电学测量,使我们能够探索金属掺杂对纳米棒电学性能的影响。
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引用次数: 0
Platinum Nanotubes Calculated Using Relativistic Cylindrical Wave Technique: Chiral Induced Spin Selectivity 用相对论柱面波技术计算铂纳米管:手性诱导自旋选择性
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-03-30 DOI: 10.35745/afm2023v03.01.0001
P. D’yachkov, E. D’yachkov
Electronic and spin properties of chiral platinum nanotubes are calculated using the relativistic linear augmented cylindrical waves method. The spin-orbit coupling induces the strong splitting of nonrelativistic dispersion curves for the Fermi energy region. The large differences in spin densities of states for spins up and down can be used to create pure spin currents through the tubules. In the two series Pt (5, n2) and Pt (10, n2), the (5, 3) and (10, 7) nanotubes show the strongest chirality-induced spin selectivity effects.
利用相对论线性增广柱面波方法计算了手性铂纳米管的电子和自旋性质。自旋轨道耦合引起了费米能区非相对论色散曲线的强烈分裂。上下自旋状态的自旋密度的巨大差异可以用来产生通过小管的纯自旋电流。在Pt (5, n2)和Pt (10, n2)两个系列中,(5,3)和(10,7)纳米管表现出最强的手性诱导自旋选择性效应。
{"title":"Platinum Nanotubes Calculated Using Relativistic Cylindrical Wave Technique: Chiral Induced Spin Selectivity","authors":"P. D’yachkov, E. D’yachkov","doi":"10.35745/afm2023v03.01.0001","DOIUrl":"https://doi.org/10.35745/afm2023v03.01.0001","url":null,"abstract":"Electronic and spin properties of chiral platinum nanotubes are calculated using the relativistic linear augmented cylindrical waves method. The spin-orbit coupling induces the strong splitting of nonrelativistic dispersion curves for the Fermi energy region. The large differences in spin densities of states for spins up and down can be used to create pure spin currents through the tubules. In the two series Pt (5, n2) and Pt (10, n2), the (5, 3) and (10, 7) nanotubes show the strongest chirality-induced spin selectivity effects.","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76924819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biodiesel Production: Agricultural and Economical Aspect in India 生物柴油生产:印度的农业和经济方面
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-03-30 DOI: 10.35745/afm2023v03.01.0002
V. Kumar, Sudhaker Dixit, Soni Gautam, Saransh Tiwari, Ajay Singh Yadav
The production of biodiesel attracts attention from scientists and researchers as of its tremendous applications and commercial use. It becomes important as of its eco-friendly nature, too, as it is biodegradable and non-polluting. The advantages of using petro-diesel with biodiesel lie in diminishing air pollution, encouraging the domestic supply of fuel, and creating new opportunities in agriculture. The production of biodiesel provides an alternative for agriculture as well as the industry. Focusing on short-duration annual crops that can generate fuel and oil seed crops provides numerous opportunities for small farmers. The intention of biodiesel concerns for creation and fortification of the environment. It is economically beneficial in several ways, namely by creating new jobs in the food and farming industries. Biodiesel fuel is eco-friendly and replaces petro-diesel. Previous studies showed that pollutants such as CO, CO2, SOX, HC, PAH, PM, and others can be reduced by using blended and pure biodiesel. However, the emission of NOX increases by using biodiesel. Biodiesel also provides a means to recycle CO2 which is mainly responsible for global warming. Biodiesel has been produced using plant oils such as Jatropha oil, Cottonseed oil, Pongamia oil, Palm oils, Rapeseed oil, and Castor oil. Such oils are converted to biodiesel through transesterification. We investigated the potential of Jatropha oil as a source of biodiesel. Biodiesel has become an eco-friendly and alternative fuel with many aspects in industrial use. When the use of biodiesel becomes more popular than petroleum fuel, the supply must be sufficient to meet daily needs. Therefore, the presented review article describes production, properties, agricultural benefits, marketing, and the prospect of biodiesel.
生物柴油的生产因其巨大的应用和商业用途而受到科学家和研究人员的关注。它的生态友好性也变得很重要,因为它是可生物降解的,无污染的。使用汽油柴油和生物柴油的优点在于减少空气污染,鼓励国内燃料供应,并为农业创造新的机会。生物柴油的生产为农业和工业提供了另一种选择。专注于可以生产燃料和油料种子作物的短周期一年生作物为小农提供了许多机会。生物柴油的目的是创造和强化环境。它在经济上有几个方面的好处,即通过在食品和农业行业创造新的就业机会。生物柴油是代替汽油柴油的环保燃料。以往的研究表明,使用混合和纯生物柴油可以减少CO、CO2、SOX、HC、PAH、PM等污染物。然而,使用生物柴油会增加氮氧化物的排放量。生物柴油还提供了一种回收二氧化碳的方法,而二氧化碳是导致全球变暖的主要原因。生物柴油是用植物油生产的,如麻风树油、棉籽油、蓬属植物油、棕榈油、菜籽油和蓖麻油。这些油通过酯交换反应转化为生物柴油。我们研究了麻疯树油作为生物柴油来源的潜力。生物柴油已成为一种具有多种工业用途的环保替代燃料。当生物柴油的使用比石油燃料更受欢迎时,其供应必须足以满足日常需求。因此,本文综述了生物柴油的生产、性质、农业效益、市场和前景。
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引用次数: 0
Slippery lubricant-infused silica nanoparticulate film processing for anti-biofouling applications. 滑滑润滑剂注入二氧化硅纳米颗粒膜处理,用于防生物污垢应用。
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-01-01 DOI: 10.1177/22808000231184688
Yuen Yee Li Sip, Annabel Jacobs, Alejandra Morales, Mengdi Sun, Luke B Roberson, Mary E Hummerick, Herve Roy, Pieter Kik, Lei Zhai

Microbial biofilm build-up in water distribution systems can pose a risk to human health and pipe material integrity. The impact is more devastating in space stations and to astronauts due to the isolation from necessary replacement parts and medical resources. As a result, there is a need for coatings to be implemented onto the inner region of the pipe to minimize the adherence and growth of biofilms. Lubricant-infused surfaces has been one such interesting material for anti-biofouling applications in which their slippery property promotes repellence to many liquids and thus prevents bacterial adherence. Textured and porous films are suitable substrate candidates to infuse and contain the lubricant. However, there is little investigation in utilizing a nanoparticulate thin film as the substrate material for lubricant infusion. A nanoparticulate film has high porosity within the structure which can promote greater lubricant infusion and retention. The implementation as a thin film structure aids to reduce material consumption and cost. In our study, we utilized a well-studied nanoporous thin film fabricated via layer-by-layer assembly of polycations and colloid silica and then calcination for greater stability. The film was further functionalized to promote fluorinated groups and improve affinity with a fluorinated lubricant. The pristine nanoporous film was characterized to determine its morphology, thickness, wettability, and porosity. The lubricant-infused film was then tested for its lubricant layer stability upon various washing conditions and its performance against bacterial biofilm adherence as a result of its slippery property. Overall, the modified silica nanoparticulate thin film demonstrated potential as a base substrate for lubricant-infused surface fabrication that repelled against ambient aqueous solvents and as an anti-biofouling coating that demonstrated low biofilm coverage and colony forming unit values. Further optimization to improve lubricant retention or incorporation of a secondary function can aid in developing better coatings for biofilm mitigation.

配水系统中的微生物生物膜堆积会对人类健康和管道材料的完整性构成风险。由于与必要的更换部件和医疗资源隔离,这种影响在空间站和宇航员中更具破坏性。因此,需要在管道的内部区域涂覆涂层,以最大限度地减少生物膜的粘附和生长。注入润滑剂的表面是抗生物污垢应用中一种非常有趣的材料,在这种材料中,它们的光滑特性促进了对许多液体的排斥,从而防止细菌粘附。有纹理和多孔的薄膜是注入和含有润滑剂的合适的候选基材。然而,利用纳米颗粒薄膜作为润滑剂注入的基底材料的研究很少。纳米颗粒膜在结构内具有高孔隙率,这可以促进更大的润滑剂注入和保留。作为薄膜结构的实现有助于减少材料消耗和成本。在我们的研究中,我们使用了一种经过充分研究的纳米多孔薄膜,该薄膜通过聚阳离子和胶体二氧化硅的逐层组装,然后煅烧以获得更大的稳定性。该膜被进一步官能化以促进氟化基团并提高与氟化润滑剂的亲和力。对原始纳米多孔膜进行了表征,以确定其形态、厚度、润湿性和孔隙率。然后测试注入润滑剂的膜在各种洗涤条件下的润滑剂层稳定性,以及由于其光滑特性而抗细菌生物膜粘附的性能。总的来说,改性的二氧化硅纳米颗粒薄膜显示出作为润滑剂注入表面制造的基底基底的潜力,该基底对周围的水性溶剂具有排斥作用,并且作为抗生物污垢涂层显示出低生物膜覆盖率和集落形成单位值。进一步优化以提高润滑剂保留率或结合二级功能可以帮助开发更好的用于生物膜缓解的涂层。
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引用次数: 0
Impact of a staggered scaffold structure on the mechanical properties and cell response in bone tissue engineering. 交错支架结构对骨组织工程中力学性能和细胞反应的影响。
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-01-01 DOI: 10.1177/22808000231181326
Xiaoli He, Qian Zhao, Ningning Zhang, Junbin Wang, Qingzong Si, Ying Xue, Zhe Xing

The primary goal of bone tissue engineering is to fabricate scaffolds that can provide a microenvironment similar to that of natural bone. Therefore, various scaffolds have been designed to replicate the bone structure. Although most tissues exhibit complicated structures, their basic structural unit includes stiff platelets arranged in a staggered micro-array. Therefore, many researchers have designed scaffolds with staggered patterns. However, relatively few studies have comprehensively analyzed this type of scaffold. In this review, we have analyzed scientific research pertaining to staggered scaffold designs and summarized their effects on the physical and biological properties of scaffolds. Compression tests or finite element analysis are typically used to evaluate the mechanical properties of scaffolds, and most studies have performed experiments in cell cultures. Staggered scaffolds improve mechanical strength and are beneficial for cell attachment, proliferation, and differentiation in comparison with conventional designs. However, very few have been studied in vivo experiments. Additionally, studies on the effect of staggered structures on angiogenesis or bone regeneration in vivo, particularly in large animals, are required. Currently, with the prevalence of artificial intelligence (AI)-based technologies, highly optimized models can be developed, resulting in better discoveries. In the future, AI can be used to deepen our understanding on the staggered structure, promoting its use in clinical applications.

骨组织工程的主要目标是制造能够提供与天然骨相似的微环境的支架。因此,人们设计了各种各样的支架来复制骨结构。尽管大多数组织结构复杂,但其基本结构单位包括排列在交错微阵列中的僵硬血小板。因此,许多研究人员设计了交错模式的支架。然而,对这类支架进行全面分析的研究相对较少。在本文中,我们分析了有关交错支架设计的科学研究,并总结了交错支架设计对支架物理和生物性能的影响。压缩试验或有限元分析通常用于评估支架的力学性能,大多数研究都是在细胞培养中进行的实验。与传统支架设计相比,交错支架提高了机械强度,有利于细胞附着、增殖和分化。然而,很少有体内实验研究。此外,还需要研究交错结构对体内血管生成或骨再生的影响,特别是在大型动物中。目前,随着基于人工智能(AI)技术的普及,可以开发高度优化的模型,从而获得更好的发现。未来,人工智能可以加深我们对交错结构的理解,促进其在临床应用中的应用。
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引用次数: 0
An investigation of a self-assembled cell-extracellular complex and its potentials in improving wound healing. 一种自组装细胞-细胞外复合物的研究及其在促进伤口愈合方面的潜力。
IF 2.5 4区 医学 Q2 Biochemistry, Genetics and Molecular Biology Pub Date : 2023-01-01 DOI: 10.1177/22808000221130168
Danyan Ye, Yaowen Sun, Lujun Yang, Jing Su

Background: To maintain and enhance the wound healing effects of mesenchymal stem cells (MSCs), a scaffold for hosting MSCs is needed, which ought to be completely biocompatible, durable, producible, and of human source.

Objective: To build a cell-extracellular matrix (ECM) complex assembled by human umbilical cord mesenchymal stem cells (HuMSCs) and to investigate its clinical potentials in promoting wound healing.

Method: HuMSCs were isolated and expanded. When the cells of third passage reached confluency, ascorbic acid was added to stimulate the cells to deposit ECM where the cells grew in. Four weeks later, a cells-loaded ECM sheet was formed. The cell-ECM complex was observed under the scanning electron microscopy (SEM) and subjected to histological studies. The supernatants were collected and the cell-ECM complex was harvested at different time points and processed for enzyme-linked immune sorbent assay (ELISA) and mRNA analysis. The in vivo experiments were performed by means of implanting the cell-ECM complex on the mice back for up to 6 months and the specimens were collected for histological studies.

Results: After 4 weeks of cultivation with ascorbic stimulation, a sheet was formed which is mainly composed with HuMSCs, collagen and hyaluronic acid. The cell-ECM complex can sustain to certain tensile force. The mRNA and protein levels of vascular endothelial growth factor-α (VEGF-α), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), and transforming growth factor-β1 (TGF-β1) were remarkably increased compared to monolayer-cultured cells. The implanted cell-ECM complex on mice was still noticeable with host cells infiltration and vascularization on 6 months.

Conclusion: Our studies suggested that HuMSCs can be multi-cultivated through adding ascorbic stimulation and ECM containing collagen and hyaluronic acid were enriched around the cells which self-assembly formed a cell-ECM complex. Cell-ECM complex can improve growth factors secretion remarkably which means it may promote wound healing by paracrine.

背景:为了维持和增强间充质干细胞(MSCs)的伤口愈合效果,需要一种具有完全生物相容性、耐用性、可生产性和人源性的支架来承载MSCs。目的:构建人脐带间充质干细胞(HuMSCs)组装的细胞-细胞外基质(ECM)复合物,探讨其促进创面愈合的临床潜力。方法:分离并扩增HuMSCs。当第三代细胞达到融合时,加入抗坏血酸刺激细胞在细胞生长的地方沉积ECM。四周后,细胞负载的ECM片形成。在扫描电镜下观察细胞- ecm复合物,并进行组织学研究。收集上清液,在不同时间点收集细胞- ecm复合物,并进行酶联免疫吸附试验(ELISA)和mRNA分析。体内实验通过将细胞- ecm复合物植入小鼠背部长达6个月的方法进行,并收集标本进行组织学研究。结果:抗坏血酸刺激培养4周后,形成以HuMSCs、胶原蛋白和透明质酸为主的膜层。细胞- ecm复合物能维持一定的拉伸力。与单层培养细胞相比,血管内皮生长因子-α (VEGF-α)、肝细胞生长因子(HGF)、角质细胞生长因子(KGF)和转化生长因子-β1 (TGF-β1) mRNA和蛋白水平显著升高。6个月时,移植细胞- ecm复合物在小鼠体内仍有明显的宿主细胞浸润和血管形成。结论:我们的研究表明,通过添加抗坏血酸刺激,HuMSCs可以进行多次培养,并且在细胞周围富集了含有胶原和透明质酸的ECM,并自组装形成细胞-ECM复合物。细胞- ecm复合物能显著提高生长因子的分泌,可能通过旁分泌促进伤口愈合。
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引用次数: 0
期刊
Journal of Applied Biomaterials & Functional Materials
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