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Effect of titanium dioxide on Streptococcus mutans biofilm. 二氧化钛对变形链球菌生物膜的影响。
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2023-01-01 DOI: 10.1177/22808000221131892
Molly K Sanders, Simone Duarte, Hadeel M Ayoub, Allison C Scully, LaQuia A Vinson, Richard L Gregory

Background: Streptococcus mutans (S. mutans) participates in the dental caries process. Titanium dioxide (TiO2) nanoparticles produce reactive oxygen species capable of disrupting bacterial DNA synthesis by creating pores in cell walls and membranes.

Objective: The objective of this study was to determine the effect of TiO2 on the disruption of S. mutans biofilm.

Methods: This study was conducted in four phases involving a TiO2-containing toothbrush and TiO2 nanoparticles. Each phase was completed using 24 h established S. mutans biofilm growth. Phase one data was collected through a bacterial plating study, assessing biofilm viability. Biofilm mass was evaluated in phase two of the study by measuring S. mutans biofilm grown on microtiter plates following crystal violet staining. The third phase of the study involved a generalized oxygen radical assay to determine the relative amount of oxygen radicals released intracellularly. Phase four of the study included the measurement of insoluble glucan/extracellular polysaccharide (EPS) synthesis using a phenol-sulfuric acid assay.

Results: Both exposure time and time intervals had a significant effect on bacterial viability counts (p = 0.0323 and p = 0.0014, respectively). Bacterial counts after 6 min of exposure were significantly lower than after 2 min (p = 0.034), compared to the no treatment control (p = 0.0056). As exposure time increased, the amount of remaining biofilm mass was statistically lower than the no treatment control. Exposure time had a significant effect on oxygen radical production. Both the 30 and 100 nm TiO2 nanoparticles had a significant effect on bacterial mass. The silver nanoparticles and the 30 and 100 nm TiO2 nanoparticles significantly inhibited EPS production.

Conclusion: The TiO2-containing toothbrush kills, disrupts, and produces oxygen radicals that disrupt established S. mutans biofilm. TiO2 and silver nanoparticles inhibit EPS production and reduce biofilm mass. The addition of TiO2 to dental products may be effective in reducing cariogenic dental biofilm.

背景:变形链球菌(S. mutans)参与蛀牙过程。二氧化钛(TiO2)纳米粒子通过在细胞壁和细胞膜上形成孔,产生能够破坏细菌DNA合成的活性氧。目的:本研究的目的是确定TiO2对变形链球菌生物膜破坏的影响。方法:采用含TiO2牙刷和TiO2纳米颗粒共4个阶段进行研究。每个阶段使用24 h建立的变形链球菌生物膜生长完成。第一阶段数据通过细菌电镀研究收集,评估生物膜的活力。在第二阶段的研究中,通过测量结晶紫染色后在微滴板上生长的变形链球菌生物膜来评估生物膜质量。研究的第三阶段包括一个广义的氧自由基测定,以确定细胞内释放的氧自由基的相对数量。研究的第四阶段包括使用苯酚-硫酸法测量不溶性葡聚糖/细胞外多糖(EPS)的合成。结果:暴露时间和时间间隔对细菌活力计数有显著影响(p = 0.0323和p = 0.0014)。暴露6 min后细菌计数显著低于暴露2 min后(p = 0.034),与未处理对照组相比(p = 0.0056)。随着暴露时间的增加,剩余生物膜质量的数量在统计学上低于未处理的对照组。暴露时间对氧自由基产生有显著影响。30 nm和100 nm TiO2纳米颗粒对细菌质量均有显著影响。银纳米粒子和30 nm和100 nm TiO2纳米粒子显著抑制EPS的产生。结论:含tio2牙刷杀死、破坏并产生氧自由基,破坏已建立的变形链球菌生物膜。TiO2和银纳米粒子抑制EPS的产生,降低生物膜质量。在口腔产品中添加TiO2可以有效地减少牙齿生物膜的形成。
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引用次数: 0
Study of EGCG composite hydrogel for the treatment of radiation-induced skin injuries. EGCG 复合水凝胶治疗辐射引起的皮肤损伤的研究。
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2023-01-01 DOI: 10.1177/22808000231218996
Jingying Wang, Lin Gao, Jianbo Song, Sijin Li

With the rising incidence of cancer, radiotherapy has become an increasingly popular treatment modality. However, radiation-induced skin injuries (RSIs) have emerged as a commonly reported side effect of radiotherapy, thereby presenting a significant challenge in the field of radiotherapy. In this study, we report the successful synthesis of a photosensitive hydrogel via amide reaction for grafting the photosensitive group, methacrylate anhydride (MA), onto chitosan (CHI) and gelatin (GEL), with subsequent physical incorporation of epigallocatechin gallate (EGCG). The resulting composite photosensitive hydrogels exhibited favorable swelling properties, rheological properties, and biocompatibility, which promote angiogenesis and demonstrate notable therapeutic efficacy against RSIs. These findings provide valuable insights into the clinical utility of EGCG composite hydrogels for the effective management of RSIs.

随着癌症发病率的上升,放射治疗已成为一种日益流行的治疗方式。然而,放疗引起的皮肤损伤(RSIs)已成为放疗的常见副作用,从而给放疗领域带来了巨大挑战。在这项研究中,我们报告了通过酰胺反应将光敏基团甲基丙烯酸甲酯酐(MA)接枝到壳聚糖(CHI)和明胶(GEL)上,并在其中加入表没食子儿茶素没食子酸酯(EGCG),从而成功合成了一种光敏水凝胶。由此产生的复合光敏水凝胶具有良好的溶胀特性、流变特性和生物相容性,可促进血管生成,对 RSI 具有显著疗效。这些发现为 EGCG 复合水凝胶有效治疗 RSI 的临床应用提供了宝贵的见解。
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引用次数: 0
Cumulative antitumor effect of bismuth lipophilic nanoparticles and cetylpyridinium chloride in inhibiting the growth of lung cancer. 亲脂铋纳米颗粒与十六烷基吡啶氯化抑制肺癌生长的累积抗肿瘤作用。
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2023-01-01 DOI: 10.1177/22808000231161177
Claudia María García-Cuellar, Rene Hernández-Delgadillo, Jesús Alejandro Torres-Betancourt, Juan Manuel Solis-Soto, Irene Meester, Yesennia Sánchez-Pérez, Nayely Pineda-Aguilar, Sergio Eduardo Nakagoshi-Cepeda, Rosa Isela Sánchez-Nájera, María Argelia Akemi Nakagoshi-Cepeda, Shankararaman Chellam, Claudio Cabral-Romero

Objective: To determine the combined antitumor effect of bismuth lipophilic nanoparticles (BisBAL NP) and cetylpyridinium chloride (CPC) on human lung tumor cells.

Material and methods: The human lung tumor cells A549 were exposed to 1-100 µM BisBAL NP or CPC, either separately or in a 1:1 combination. Cell viability was measured with the PrestoBlue assay, the LIVE/DEAD assay, and fluorescence microscopy. The integrity and morphology of cellular microtubules were analyzed by immunofluorescence.

Results: A 24-h exposure to 1 µM solutions reduced A549 growth with 21.5% for BisBAL NP, 70.5% for CPC, and 92.4% for the combination (p < 0.0001), while a 50 µM BisBAL NP/CPC mixture inhibited cell growth with 99% (p < 0.0001). BisBAL NP-curcumin conjugates were internalized within 30 min of exposure and could be traced within the nucleus of tumor cells within 2 h. BisBAL NP, but not CPC, interfered with microtubule organization, thus interrupting cell replication, similar to the action mechanism of docetaxel.

Conclusion: The growth inhibition of A549 human tumor cells by BisBAL NP and CPC was cumulative as of 1 µM. The BisBAL NP/CPC combination may constitute an innovative and cost-effective alternative for treating human lung cancer.

目的:探讨亲脂铋纳米粒子(BisBAL NP)与氯化十六烷基吡啶(CPC)对人肺肿瘤细胞的联合抑瘤作用。材料和方法:将人肺肿瘤细胞A549分别暴露于1-100µM BisBAL NP或CPC中,或以1:1的比例组合。采用PrestoBlue法、LIVE/DEAD法和荧光显微镜检测细胞活力。免疫荧光分析细胞微管的完整性和形态。结果:暴露于1µM溶液24 h, BisBAL NP对A549细胞的生长抑制率为21.5%,CPC为70.5%,联合作用为92.4% (p p)。结论:BisBAL NP和CPC对A549人肿瘤细胞的生长抑制作用自1µM开始累积。BisBAL NP/CPC组合可能是治疗人类肺癌的一种创新且具有成本效益的替代方案。
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引用次数: 0
Anti-fungal efficacy of Miswak Extract (Salvadora Persica) and commercial cleaner against Candida albicans on heat cured polymethylmethacrylate denture base. Miswak 提取物(Salvadora Persica)和商用清洁剂对热固化聚甲基丙烯酸甲酯义齿基托上白色念珠菌的抗真菌功效。
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2023-01-01 DOI: 10.1177/22808000231165666
Nadia Wajdan, Kashif Aslam, Rafat Amin, Sadaf Khan, Naseer Ahmed, Abhishek Lal, Eman M AlHamdan, Fahim Vohra, Tariq Abduljabbar, Artak Heboyan

Aim: The aim of the study was to compare the antifungal efficacy between commercial cleaner (Corega) and Miswak extract (Salvadora persica) against Candida albicans on heat cured Polymethyl methacrylate (PMMA) acrylic denture base resin.

Materials and methods: Forty-eight samples of heat cured PMMA acrylic denture base resin were fabircated in the study. The sterile acrylic resin specimens were immersed in standardized cell suspension of Candida albicans and incubated for 60 min at 370°C for cell adhesion and 2 h at 370°C for biofilm formation. After 24 h biofilm was evaluated by cell viability (CFUs) on SDA and cell counting of Candida albicans under light microscope at 400× magnification. The fungicidal effect of commercial cleaner and Miswak extract on Candida albicans biofilm was then evaluated by colony-forming units on SDA and cell counting under light microscope at 400× magnification.

Results: Screening test agar disk-diffusion assay showed mean inhibitory zone of 3 mm for commercial cleaner as compared to Miswak extract, which showed mean inhibitory zone of 2 and 1 mm for different concentrations. Broth microdilution method showed 31 mg/ml MIC and 62.5 mg/ml Minimal Fungicidal Concentration (MFC) values for commercial cleaner as compared to Miswak extract that showed 125 mg/ml MIC and 250 mg/ml MFC values against Candida albicans. A significant difference (p < 0.05) was observed between pre and post treatment of both commercial cleaner and Miswak extract, for CFUs and cell count for Candida albicans.

Conclusion: Commercial denture cleaner (Corega) showed better antifungal (C albicans) activity than Miswak extract (Salvadora persica) on heat cured PMMA acrylic denture base resin.

目的:本研究旨在比较商用清洁剂(Corega)和 Miswak 提取物(Salvadora persica)对热固化聚甲基丙烯酸甲酯(PMMA)丙烯酸义齿基托树脂上白色念珠菌的抗真菌功效:本研究共制作了 48 份热固化 PMMA 丙烯酸义齿基托树脂样本。将无菌丙烯酸树脂试样浸入白色念珠菌的标准细胞悬浮液中,在 370°C 下培养 60 分钟以检测细胞粘附情况,在 370°C 下培养 2 小时以检测生物膜形成情况。24 小时后,通过 SDA 上的细胞存活率(CFUs)和 400 倍放大镜下的白色念珠菌细胞计数对生物膜进行评估。然后,通过 SDA 上的菌落形成单位和 400 倍放大光镜下的细胞计数,评估商用清洁剂和 Miswak 提取物对白念珠菌生物膜的杀菌效果:筛选试验琼脂盘扩散法显示,商用清洁剂的平均抑制区为 3 毫米,而不同浓度的 Miswak 提取物的平均抑制区分别为 2 毫米和 1 毫米。肉汤微量稀释法显示,商用清洁剂的 MIC 值为 31 毫克/毫升,最低杀菌浓度 (MFC) 值为 62.5 毫克/毫升,而 Miswak 提取物对白色念珠菌的 MIC 值为 125 毫克/毫升,最低杀菌浓度 (MFC) 值为 250 毫克/毫升。商用清洁剂和 Miswak 提取物在处理前和处理后对白色念珠菌的 CFUs 和细胞数都有明显差异(p < 0.05):在热固化 PMMA 丙烯酸义齿基底树脂上,商用义齿清洁剂(Corega)比 Miswak 提取物(Salvadora persica)显示出更好的抗真菌(白色念珠菌)活性。
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引用次数: 0
Influence of carbon and graphene oxide nanoparticle on the adhesive properties of dentin bonding polymer: A SEM, EDX, FTIR study. 碳和氧化石墨烯纳米粒子对牙本质粘接聚合物粘接性能的影响:SEM、EDX、FTIR 研究。
IF 3.1 4区 医学 Q2 BIOPHYSICS Pub Date : 2023-01-01 DOI: 10.1177/22808000231159238
Hanan Alsunbul, Yasser F Alfawaz, Eman M Alhamdan, Imran Farooq, Fahim Vohra, Tariq Abduljabbar

Objective: This study was aimed at including 2.5 wt.% of carbon nanoparticles (CNPs) and graphene oxide NPs (GNPs) in a control adhesive (CA) and then investigate the effect of this inclusion on their mechanical properties and its adhesion to root dentin.

Materials and methods: Scanning electron microscopy and energy dispersive X-ray (SEM-EDX) mapping were conducted to investigate the structural features and elemental distribution of CNPs and GNPs, respectively. These NPs were further characterized by Raman spectroscopy. The adhesives were characterized by evaluating their push-out bond strength (PBS), rheological properties, degree of conversion (DC) investigation, and failure type analysis.

Results: The SEM micrographs revealed that the CNPs were irregular and hexagonal, whereas the GNPs were flake-shaped. EDX analysis showed that carbon (C), oxygen (O), and zirconia (Zr) were found in the CNPs, while the GNPs were composed of C and O. The Raman spectra for CNPs and GNPs revealed their characteristic bands (CNPs-D band: 1334 cm-1, GNPs-D band: 1341 cm-1, CNPs-G band: 1650 cm-1, and GNPs-G band: 1607 cm-1). The testing verified that the highest bond strength to root dentin were detected for GNP-reinforced adhesive (33.20 ± 3.55 MPa), trailed closely by CNP-reinforced adhesive (30.48 ± 3.10 MPa), while, the CA displayed lowest values (25.11 ± 3.60 MPa). The inter-group comparisons of the NP-reinforced adhesives with the CA revealed statistically significant results (p < 0.01). Failures of adhesive nature were most common in within the adhesives and root dentin. The rheological assessment results demonstrated a reduced viscosity for all the adhesives observed at advanced angular frequencies. All the adhesives verified suitable dentin interaction shown by hybrid layer and appropriate resin tag development. A reduced DC was perceived for both NP-reinforced adhesives, compared to the CA.

Conclusion: The present study's findings have demonstrated that 2.5% GNP adhesive revealed the highest, suitable root dentin interaction, and acceptable rheological properties. Nevertheless, a reduced DC was observed (matched with the CA). Prospective studies probing the influence of diverse concentrations of filler NPs on the adhesive's mechanical properties to root dentin are recommended.

研究目的本研究的目的是在对照粘合剂(CA)中加入 2.5 wt.%的碳纳米粒子(CNPs)和氧化石墨烯纳米粒子(GNPs),然后研究加入这两种纳米粒子对其机械性能及其与牙根粘合力的影响:扫描电子显微镜和能量色散 X 射线(SEM-EDX)制图分别研究了 CNPs 和 GNPs 的结构特征和元素分布。拉曼光谱对这些 NPs 做了进一步表征。粘合剂的表征方法包括评估其推出粘合强度(PBS)、流变特性、转化率(DC)调查和失效类型分析:扫描电镜显微照片显示 CNPs 呈不规则六边形,而 GNPs 则呈片状。拉曼光谱显示了 CNPs 和 GNPs 的特征带(CNPs-D 带:1334 cm-1,GNPs-D 带:1341 cm-1,CNPs-G 带:1650 cm-1,GNPs-G 带:1607 cm-1)。测试证实,GNP 增强型粘合剂与牙根的粘合强度最高(33.20 ± 3.55 兆帕),CNP 增强型粘合剂紧随其后(30.48 ± 3.10 兆帕),而 CA 粘合剂的粘合强度值最低(25.11 ± 3.60 兆帕)。NP 增强型粘合剂与 CA 的组间比较显示出具有统计学意义的结果(p 结论:NP 增强型粘合剂与 CA 的组间比较显示出具有统计学意义的结果:本研究结果表明,2.5% GNP 粘合剂显示出最高的、合适的根牙本质相互作用和可接受的流变特性。尽管如此,还是观察到了较低的直流电(与 CA 相匹配)。建议进行前瞻性研究,探讨不同浓度的填充 NP 对粘合剂与牙根的机械性能的影响。
{"title":"Influence of carbon and graphene oxide nanoparticle on the adhesive properties of dentin bonding polymer: A SEM, EDX, FTIR study.","authors":"Hanan Alsunbul, Yasser F Alfawaz, Eman M Alhamdan, Imran Farooq, Fahim Vohra, Tariq Abduljabbar","doi":"10.1177/22808000231159238","DOIUrl":"10.1177/22808000231159238","url":null,"abstract":"<p><strong>Objective: </strong>This study was aimed at including 2.5 wt.% of carbon nanoparticles (CNPs) and graphene oxide NPs (GNPs) in a control adhesive (CA) and then investigate the effect of this inclusion on their mechanical properties and its adhesion to root dentin.</p><p><strong>Materials and methods: </strong>Scanning electron microscopy and energy dispersive X-ray (SEM-EDX) mapping were conducted to investigate the structural features and elemental distribution of CNPs and GNPs, respectively. These NPs were further characterized by Raman spectroscopy. The adhesives were characterized by evaluating their push-out bond strength (PBS), rheological properties, degree of conversion (DC) investigation, and failure type analysis.</p><p><strong>Results: </strong>The SEM micrographs revealed that the CNPs were irregular and hexagonal, whereas the GNPs were flake-shaped. EDX analysis showed that carbon (C), oxygen (O), and zirconia (Zr) were found in the CNPs, while the GNPs were composed of C and O. The Raman spectra for CNPs and GNPs revealed their characteristic bands (CNPs-D band: 1334 cm<sup>-1</sup>, GNPs-D band: 1341 cm<sup>-1</sup>, CNPs-G band: 1650 cm<sup>-1</sup>, and GNPs-G band: 1607 cm<sup>-1</sup>). The testing verified that the highest bond strength to root dentin were detected for GNP-reinforced adhesive (33.20 ± 3.55 MPa), trailed closely by CNP-reinforced adhesive (30.48 ± 3.10 MPa), while, the CA displayed lowest values (25.11 ± 3.60 MPa). The inter-group comparisons of the NP-reinforced adhesives with the CA revealed statistically significant results (<i>p</i> < 0.01). Failures of adhesive nature were most common in within the adhesives and root dentin. The rheological assessment results demonstrated a reduced viscosity for all the adhesives observed at advanced angular frequencies. All the adhesives verified suitable dentin interaction shown by hybrid layer and appropriate resin tag development. A reduced DC was perceived for both NP-reinforced adhesives, compared to the CA.</p><p><strong>Conclusion: </strong>The present study's findings have demonstrated that 2.5% GNP adhesive revealed the highest, suitable root dentin interaction, and acceptable rheological properties. Nevertheless, a reduced DC was observed (matched with the CA). Prospective studies probing the influence of diverse concentrations of filler NPs on the adhesive's mechanical properties to root dentin are recommended.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":"21 ","pages":"22808000231159238"},"PeriodicalIF":3.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9453234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bovine hydroxyapatite-based scaffold accelerated the inflammatory phase and bone growth in rats with bone defect. 牛羟基磷灰石支架促进骨缺损大鼠炎症期和骨生长。
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2023-01-01 DOI: 10.1177/22808000221149193
Maria Apriliani Gani, Aniek Setiya Budiatin, Dewi Wara Shinta, Chrismawan Ardianto, Junaidi Khotib

Hydroxyapatite (HA) is a biomaterial widely used to treat bone defect, such as due to traffic accident. The HA scaffold is obtained from synthetic HA or natural sources, such as bovine hydroxyapatite (BHA). This study aims to compare the characteristics and in vivo performance of BHA-based and HA-based scaffolds. For this purpose, the scaffold was formulated with gelatin (GEL) and characterised by SEM-EDX, FTIR and mini autograph. The defect model was carried out on the femur area of Wistar rats classified into three animal groups: defect, HA-GEL and BHA-GEL. Postoperatively (7, 14 and 28 days), the bone was radiologically evaluated, and stained with haematoxylin-eosin, anti-CD80 and anti-CD163. The BHA-GEL scaffold showed a regular surface and spherical particle shape, whereas the HA-GEL scaffold exhibited irregular surface. The BHA-GEL scaffold had higher pore size and compressive strength and lower calcium-to-phosphorus ratio than the HA-GEL scaffold. In vivo study showed that the expression of CD80 in the three experimental groups was not significantly different. However, the expression of CD163 differed significantly between the groups. The BHA-GEL group showed robust expression of CD163 on day 7, which rapidly decreased over time. It also showed increased osteoclasts, osteoblasts and osteocytes cell count that contributed to the integrity of the defect area. In conclusion, the BHA-based scaffold exhibited the desired physical and chemical characteristics that benefit in vivo performance versus the HA-based scaffold. Thus, the BHA-based scaffold may be used as a bone graft.

羟基磷灰石(Hydroxyapatite, HA)是一种广泛用于治疗交通事故等骨缺损的生物材料。透明质酸支架可从合成透明质酸或天然来源获得,如牛羟基磷灰石(BHA)。本研究旨在比较bha基支架和ha基支架的特点和体内性能。为此,支架用明胶(GEL)配制,并通过SEM-EDX, FTIR和迷你签名进行表征。在Wistar大鼠股骨区域建立缺损模型,分为缺损组、HA-GEL组和BHA-GEL组。术后(7天、14天和28天)对骨进行放射学评估,并用血红素-伊红染色、抗cd80和抗cd163染色。BHA-GEL支架表面呈规则形状,颗粒呈球形,而HA-GEL支架表面呈不规则形状。与HA-GEL支架相比,BHA-GEL支架的孔径和抗压强度更高,钙磷比更低。体内研究表明,CD80在三个实验组中的表达无显著差异。然而,CD163的表达在两组之间存在显著差异。BHA-GEL组在第7天表现出强烈的CD163表达,随着时间的推移迅速下降。它还显示破骨细胞、成骨细胞和骨细胞数量的增加有助于缺损区域的完整性。总之,与ha基支架相比,bha基支架表现出所需的物理和化学特性,有利于体内性能。因此,基于bha的支架可以用作骨移植。
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引用次数: 1
Evaluation of the biocompatibility of fish skin collagen with the mesenchymal stem cells in in vitro cultures. 评估鱼皮胶原与间充质干细胞在体外培养中的生物相容性。
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2023-01-01 DOI: 10.1177/22808000231211423
Kinga Rusinek, Anna Słysz, Tomasz Dębski, Katarzyna Siennicka, Aleksandra Zołocińska, Justyna Miszkiewicz-Jasińska, Anna Aleksandrowicz, Zygmunt Pojda

There are scarce published data suggesting, that collagen extracted from fish skin may be an attractive alternative to mammalian-derived collagen for the in vitro cell cultures. In this study, we investigated proliferation potential and differentiation capability into osteogenic and adipogenic lineages of rat adipose-derived mesenchymal stem cells (rASCs) and human adipose-derived mesenchymal stem cells (hASCs) cultured on collagen extracted from silver carp and African sharptooth catfish skins, compared with commercially available mammalian collagen and collagen-free culture dishes. Our results revealed no significant differences between fish collagen and mammalian collagen in supporting cell viability and proliferation capacity. Fish-derived collagen is a cheap material derived from production waste, does not contain transmissible pathogens of mammalian origin, supports human cell cultures at comparable level to conventional collagen sources, and may be considered as the product of choice for the in vitro cell cultures.

已发表的数据表明,从鱼皮中提取的胶原蛋白可能是哺乳动物来源胶原蛋白的一种有吸引力的体外细胞培养替代物。在这项研究中,我们研究了大鼠脂肪间充质干细胞(rASCs)和人脂肪间充质干细胞(hASCs)在鲢鱼皮和非洲尖齿鲶鱼皮提取的胶原蛋白上与市售哺乳动物胶原蛋白和无胶原蛋白培养皿相比的增殖潜力以及向成骨系和成脂系分化的能力。结果表明,鱼胶原蛋白与哺乳动物胶原蛋白在支持细胞活力和增殖能力方面没有明显差异。鱼源性胶原蛋白是一种从生产废料中提取的廉价材料,不含哺乳动物来源的可传播病原体,对人类细胞培养的支持水平与传统胶原蛋白来源相当,可视为体外细胞培养的首选产品。
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引用次数: 0
Spin-coating of Graphene Oxide as Counter Electrode for Dye-sensitized Solar Cells in Various Dispersing Solvents 不同分散溶剂中氧化石墨烯对电极染料敏化太阳能电池的自旋镀膜
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2022-12-30 DOI: 10.35745/afm2022v02.04.0004
T. Wu, Wei-Ming Huang, Yu-Chi Tsao, J. Tsai
Platinum (Pt) is the most commonly used counter electrode material for DSSCs. However, as Pt is a noble metal and expensive, researchers have tried to replace the Pt counter electrode with a variety of materials. In this study, graphene oxide (GO) powders were added to solvents of different polarities as precursor solutions for the counter electrode. The solvents were deionized water, dimethylformamide, isopropanol, and chlorobenzene in descending order of polarity. The prepared GO precursor solutions were coated on the Florine doped Tin Oxide (FTO) glass substrate via spin coating, then coated substrate subjected to thermal reduction reaction to obtain reduced graphene oxide, which was used as the counter electrode of the DSSC. The photoelectric conversion efficiency (PCE) of DSSC using deionized water as the dispersing solvent was 2.70%, which was five times higher than that of DSSC using chlorobenzene.
铂(Pt)是DSSCs最常用的对电极材料。然而,由于Pt是一种昂贵的贵金属,研究人员试图用各种材料代替Pt对电极。在这项研究中,氧化石墨烯(GO)粉末被添加到不同极性的溶剂中作为对电极的前驱体溶液。溶剂极性由高到低依次为去离子水、二甲基甲酰胺、异丙醇和氯苯。将制备好的氧化石墨烯前驱体溶液通过自旋涂层涂覆在氟掺杂氧化锡(FTO)玻璃基板上,再对基板进行热还原反应得到还原氧化石墨烯,作为DSSC的对电极。以去离子水为分散溶剂的DSSC的光电转换效率(PCE)为2.70%,比以氯苯为分散溶剂的DSSC提高了5倍。
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引用次数: 0
Molecular-Dynamical Investigation of Thermomechanical Properties of Spherical Solid and Hollow Nickel Nanopowder during Laser Additive Manufacturing Process 激光增材制造过程中球形固体和空心纳米镍粉末热力学性能的分子动力学研究
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2022-12-30 DOI: 10.35745/afm2022v02.04.0005
Ling-Feng Lai, Yu-Chen Su, Chun-Ming Chang, Kuei-Shu Hsu, D. Lu, Jian-Ming Lu
Molecular dynamics (MD) simulation with the embedded-atom method (EAM)/alloy potential is used to investigate the property of the nanoscale hollow spherical Nickel (Ni) powder during the laser additive manufacturing (AM) process. The thermomechanical properties of the Ni nanopowder is also explored (1) at room temperature and (2) from room temperature to the melting temperature during laser AM of powder bed fusion. As a result, the optimum parameters for the laser AM process are proposed. The optimal coalescence temperature of the nanoscale hollow spherical Ni powder is in the range between 980 and 1421K, while the melting temperature is in the range between 1320 and 1470 K. The coalescence and melting temperatures are lower than the melting point of Ni (1728 K).
采用嵌入原子法(EAM)/合金电位的分子动力学(MD)模拟研究了激光增材制造(AM)过程中纳米级空心球形镍(Ni)粉末的性能。研究了纳米Ni粉末在室温和室温至熔化温度下的热力学性能。最后,提出了激光增材加工的最佳工艺参数。纳米级空心球形Ni粉末的最佳聚结温度在980 ~ 1421K之间,熔融温度在1320 ~ 1470 K之间。熔结温度和熔点均低于Ni的熔点(1728k)。
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引用次数: 0
Direct Visualization of Si and Ge Atoms by Shifting Electron Picoscopy 用移动电子显微镜直接观察Si和Ge原子
IF 2.5 4区 医学 Q2 BIOPHYSICS Pub Date : 2022-12-30 DOI: 10.35745/afm2022v02.04.0002
O. Kucherov
The picoscopy images of the Si/Ge(100) system were analyzed, and electron cloud densitometry of silicon is presented in this study. The picoscopy is used to distinguish Ge, Si, and other chemical elements because different atoms have different densities of electron clouds. This result is in full accordance with Kucherov's law which states that the current passed through an electron cloud is proportional to the density of the cloud. The picoscopy image has shown Si crystals, Si/Ge solid solution, and their interface as the single crystal without defects. Local deformations in crystals were investigated using methods of direct visualization of individual atoms and measuring the distance of the center of atoms from the node of the crystal lattice. Visual сrystallography becomes a new way to study applied functional materials. This is the first publication on the real structure of a silicon atom.
分析了Si/Ge(100)体系的显微镜图像,提出了硅的电子云密度测定方法。由于不同的原子具有不同密度的电子云,因此使用微镜来区分锗、硅和其他化学元素。这一结果完全符合库切罗夫定律,即通过电子云的电流与电子云的密度成正比。显微镜图像显示Si晶体、Si/Ge固溶体及其界面为无缺陷的单晶。利用原子的直接可视化和原子中心到晶格节点的距离测量方法研究了晶体中的局部变形。视觉晶体学是研究应用功能材料的一种新方法。这是关于硅原子真实结构的第一份出版物。
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Journal of Applied Biomaterials & Functional Materials
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