Pub Date : 2024-01-01DOI: 10.1177/22808000241284431
Sara Bellusa, Lianrui Chu, Evelyn Fung, Kyumin Whang
Objective: To determine the effects of adding a quaternary ammonium methacryloxy silicate (K18) and K18-functionalized filler (K18-Filler) on the material and antimicrobial properties of a hard denture reline material.
Materials and methods: 30% K18 in methyl methacrylate (K18-MMA; 0-20 wt% of reliner) and K18-Filler (0-30 wt% of reliner) were incorporated into KoolinerTM hard denture reliner. KoolinerTM served as the control. The cure (Shore A hardness), hydrophilicity (contact angles), mechanical (3-point bend test), water sorption, and antimicrobial properties against Streptococcus mutans, S. sanguinis, and Candida albicans were determined.
Results: Most K18 groups cured well and had comparable Shore A hardness values (range ~52 to 70 DHN) to that of controls (67.2 ± 1.8 DHN; Bonferroni corrected p > 0.0003). Even the softest group had hardness values within the range of commercial products. Half of the K18 groups had comparable contact angles to that of controls (range ~75° to 80° vs 83.41° ± 2.66°; Bonferroni corrected p > 0.0003), and most were within the range of commercial liners. K18-MMA and K18-Filler increased modulus but decreased ultimate transverse strength (UTS). All experimental groups had comparable or higher moduli than controls (range ~966 to 2069 MPa vs 1340 ± 119 MPa; Bonferroni corrected p < 0.0003), but only half of the experimental groups had comparable UTS to that of controls (range ~41 to 49 MPa vs 55.8 ± 1.5 MPa; Bonferroni corrected p > 0.0003). The 15% and 20% K18-MMA with 30% K18-Filler groups had significant antimicrobial activity against all three microbes (p < 0.05). However, the 15% and 20% K18-MMA with 30% K18-Filler groups had significantly higher water sorption at early time points (p < 0.05). After 8 weeks, they were comparable to each other (p > 0.05).
Conclusions: K18-MMA and K18-Filler are promising antimicrobial additives that produce hard denture liners with material properties within the range of commercial products and significant antimicrobial properties against S. mutans, S. sanguinis, and C. albicans. Further development is needed to reduce water sorption.
目的材料和方法:在 KoolinerTM 硬义齿衬垫中加入甲基丙烯酸甲酯中 30% 的 K18(K18-MMA;衬垫的 0-20 wt%)和 K18-Filler(衬垫的 0-30 wt%)。KoolinerTM 作为对照。测定了固化(邵氏 A 硬度)、亲水性(接触角)、机械性(3 点弯曲试验)、吸水性以及对变异链球菌、血清链球菌和白色念珠菌的抗菌性:大多数 K18 组固化良好,与对照组(67.2 ± 1.8 DHN;Bonferroni 校正 p > 0.0003)的肖氏 A 硬度值(范围 ~52 至 70 DHN)相当。即使是最柔软的组别,其硬度值也在商用产品的范围之内。半数 K18 组的接触角与对照组相当(范围 ~75° 至 80° vs 83.41° ± 2.66°;Bonferroni 校正后 p > 0.0003),而且大多数组都在商用衬垫的范围内。K18-MMA 和 K18-Filler 增加了模量,但降低了极限横向强度 (UTS)。所有实验组的模量与对照组相当或更高(范围 ~966 至 2069 兆帕对比 1340 ± 119 兆帕;Bonferroni 校正 p p > 0.0003)。15% 和 20% K18-MMA 与 30% K18-Filler 组对所有三种微生物都具有显著的抗菌活性(p p > 0.05):结论:K18-MMA 和 K18-Filler 是很有前途的抗菌添加剂,它们生产的硬义齿衬垫的材料性能在商业产品范围内,对变异性酵母菌、血清酵母菌和白色念珠菌具有显著的抗菌性能。还需要进一步开发,以减少吸水性。
{"title":"Antimicrobial hard denture reliners using quaternary ammonium methacryloxy silicate (K18 QAMS) and K18-functionalized filler.","authors":"Sara Bellusa, Lianrui Chu, Evelyn Fung, Kyumin Whang","doi":"10.1177/22808000241284431","DOIUrl":"https://doi.org/10.1177/22808000241284431","url":null,"abstract":"<p><strong>Objective: </strong>To determine the effects of adding a quaternary ammonium methacryloxy silicate (K18) and K18-functionalized filler (K18-Filler) on the material and antimicrobial properties of a hard denture reline material.</p><p><strong>Materials and methods: </strong>30% K18 in methyl methacrylate (K18-MMA; 0-20 wt% of reliner) and K18-Filler (0-30 wt% of reliner) were incorporated into Kooliner<sup>TM</sup> hard denture reliner. Kooliner<sup>TM</sup> served as the control. The cure (Shore A hardness), hydrophilicity (contact angles), mechanical (3-point bend test), water sorption, and antimicrobial properties against <i>Streptococcus mutans</i>, <i>S. sanguinis</i>, and <i>Candida albicans</i> were determined.</p><p><strong>Results: </strong>Most K18 groups cured well and had comparable Shore A hardness values (range ~52 to 70 DHN) to that of controls (67.2 ± 1.8 DHN; Bonferroni corrected <i>p</i> > 0.0003). Even the softest group had hardness values within the range of commercial products. Half of the K18 groups had comparable contact angles to that of controls (range ~75° to 80° vs 83.41° ± 2.66°; Bonferroni corrected <i>p</i> > 0.0003), and most were within the range of commercial liners. K18-MMA and K18-Filler increased modulus but decreased ultimate transverse strength (UTS). All experimental groups had comparable or higher moduli than controls (range ~966 to 2069 MPa vs 1340 ± 119 MPa; Bonferroni corrected <i>p</i> < 0.0003), but only half of the experimental groups had comparable UTS to that of controls (range ~41 to 49 MPa vs 55.8 ± 1.5 MPa; Bonferroni corrected <i>p</i> > 0.0003). The 15% and 20% K18-MMA with 30% K18-Filler groups had significant antimicrobial activity against all three microbes (<i>p</i> < 0.05). However, the 15% and 20% K18-MMA with 30% K18-Filler groups had significantly higher water sorption at early time points (<i>p</i> < 0.05). After 8 weeks, they were comparable to each other (<i>p</i> > 0.05).</p><p><strong>Conclusions: </strong>K18-MMA and K18-Filler are promising antimicrobial additives that produce hard denture liners with material properties within the range of commercial products and significant antimicrobial properties against <i>S. mutans</i>, <i>S. sanguinis</i>, and <i>C. albicans</i>. Further development is needed to reduce water sorption.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142287923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1177/22808000241289208
Nurul Aida Ngah, Jithendra Ratnayake, George J Dias, Darryl C Tong, Siti Noor Fazliah Mohd Noor, Paul R Cooper, Haizal Mohd Hussaini
Craniofacial bone defects result from various disorders such as trauma, congenital malformations and infections. Cleft lip and palate are the most prevalent congenital craniofacial birth defect in humans. Growth factors (GFs) are soluble proteins secreted by cells that regulate various cellular processes and tissue regeneration. At present, developing three-dimensional scaffolds for delivering GFs to the site of injury has become an important aspect in craniofacial bone regeneration. This study aims to develop a novel 3D bone substitute using lyophilized-platelet-rich fibrin (LyPRF) biocomposite scaffolds for potential application for CLP repair. Collagen (C), bioglass (BG), and LyPRF were used to fabricate a biocomposite (C-BG-LyPRF) scaffold. The physical, chemical, and biocompatibility properties of the scaffold were evaluated. The C-BG-LyPRF scaffold demonstrated a mean pore diameter of 146 µm within a porosity of 87.26%. The FTIR spectra verified the presence of am-ide I, II, and III functional groups. The inorganic phase of the C-BG-LyPRF scaffold was composed of sodium, calcium, silicon, and phosphorus, as determined by EDX analysis. Furthermore, C-BG-LyPRF scaffold was biocompatible with MC3T3-E1 cells in both the Live/Dead and prolif-eration assays. Data demonstrate the developed C-BG-LyPRF scaffold exhibits biomimetic and biocompatibility properties, establishing it as a promising biomaterial for craniofacial regeneration.
{"title":"Physicochemical and biocompatibility characterisation of a 3D lyophilised platelet-rich fibrin scaffold for cleft lip and palate repair.","authors":"Nurul Aida Ngah, Jithendra Ratnayake, George J Dias, Darryl C Tong, Siti Noor Fazliah Mohd Noor, Paul R Cooper, Haizal Mohd Hussaini","doi":"10.1177/22808000241289208","DOIUrl":"https://doi.org/10.1177/22808000241289208","url":null,"abstract":"<p><p>Craniofacial bone defects result from various disorders such as trauma, congenital malformations and infections. Cleft lip and palate are the most prevalent congenital craniofacial birth defect in humans. Growth factors (GFs) are soluble proteins secreted by cells that regulate various cellular processes and tissue regeneration. At present, developing three-dimensional scaffolds for delivering GFs to the site of injury has become an important aspect in craniofacial bone regeneration. This study aims to develop a novel 3D bone substitute using lyophilized-platelet-rich fibrin (LyPRF) biocomposite scaffolds for potential application for CLP repair. Collagen (C), bioglass (BG), and LyPRF were used to fabricate a biocomposite (C-BG-LyPRF) scaffold. The physical, chemical, and biocompatibility properties of the scaffold were evaluated. The C-BG-LyPRF scaffold demonstrated a mean pore diameter of 146 µm within a porosity of 87.26%. The FTIR spectra verified the presence of am-ide I, II, and III functional groups. The inorganic phase of the C-BG-LyPRF scaffold was composed of sodium, calcium, silicon, and phosphorus, as determined by EDX analysis. Furthermore, C-BG-LyPRF scaffold was biocompatible with MC3T3-E1 cells in both the Live/Dead and prolif-eration assays. Data demonstrate the developed C-BG-LyPRF scaffold exhibits biomimetic and biocompatibility properties, establishing it as a promising biomaterial for craniofacial regeneration.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142377937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1177/22808000241251564
Le Van Hai, Do Nhu Ngoc, Pham Mai Khanh, Le Van Tuan, Vu Nhat Dinh, Nguyen Viet Nam
Objectives: This study aims to investigate the effect of coating time on the formation of hydroxyapatite (HA) coating layer on ZK60 substrate and understand the biodegradation behavior of the coated alloy for biodegradable implant applications.
Methods: Biodegradable ZK60 alloy was coated by HA layer for different times of 0.5, 1, 2, and 4 h by chemical conversion method. After coating, all the coated specimens were used for immersion test in Hanks' solution to understand the effect of coating time on the degradation behavior of the alloy. The degradation rate of the coated alloy was evaluated by Mg2+ ion quantification and pH change during immersion test. The microstructure of the coating layer was examined by scanning electron microscope (SEM) equipped with an energy-dispersive X-ray spectroscopy (EDS) before and after immersion to understand the degradation behavior of the coated alloy.
Results: HA coating layers were formed successfully on surface of ZK60 specimens after 0.5, 1, 2, and 4 h with different microstructure. Optimal coating quality was observed at 1 or 2 h, characterized by well-formed and uniform HA layers. However, extending the coating duration to 4 h led to the formation of cracks within the HA layer, accompanied by Mg(OH)2. Specimens coated for 1 and 2 h exhibited the lowest degradation rates, while specimens coated for 0.5 and 4 h showed the highest degradation rates. Furthermore, analysis of degradation products revealed the predominance of calcium phosphates formed on the surface of specimens coated for 1 and 2 h. Conversely, specimens coated for 0.5 and 4 h exhibited Mg(OH)2 as the primary degradation product, suggesting a less effective corrosion barrier under these conditions.
Conclusion: The HA layer formed after 2 h demonstrated as the most effective coating layer for enhancing the corrosion resistance of the ZK60 alloy for biomedical applications.
研究目的本研究旨在探讨涂覆时间对羟基磷灰石(HA)涂覆层在 ZK60 基底上形成的影响,并了解涂覆合金在生物降解植入物应用中的生物降解行为:方法:采用化学转化法对可生物降解的 ZK60 合金进行 0.5、1、2 和 4 h 不同时间的 HA 涂层处理。涂覆后,所有涂覆试样均在 Hanks 溶液中进行浸泡试验,以了解涂覆时间对合金降解行为的影响。在浸泡试验过程中,通过 Mg2+ 离子定量和 pH 值变化评估了涂层合金的降解率。用配备了能量色散 X 射线光谱仪(EDS)的扫描电子显微镜(SEM)观察了浸泡前后涂层的微观结构,以了解涂层合金的降解行为:0.5、1、2 和 4 小时后,不同微观结构的 ZK60 试样表面成功形成了 HA 涂层。在 1 或 2 小时内观察到最佳涂层质量,其特征是形成良好且均匀的 HA 涂层。然而,将涂层时间延长至 4 小时后,HA 层内会形成裂缝,并伴有 Mg(OH)2。涂覆时间为 1 和 2 小时的试样降解率最低,而涂覆时间为 0.5 和 4 小时的试样降解率最高。此外,对降解产物的分析表明,涂覆 1 和 2 小时的试样表面形成的主要是磷酸钙。相反,涂覆 0.5 和 4 小时的试样的主要降解产物是 Mg(OH)2,这表明在这些条件下的腐蚀屏障效果较差:结论:在生物医学应用中,2 小时后形成的 HA 层是增强 ZK60 合金耐腐蚀性最有效的涂层。
{"title":"Effect of coating time on the formation of coating layer and degradation behavior of hydroxyapatite coated ZK60 alloy.","authors":"Le Van Hai, Do Nhu Ngoc, Pham Mai Khanh, Le Van Tuan, Vu Nhat Dinh, Nguyen Viet Nam","doi":"10.1177/22808000241251564","DOIUrl":"https://doi.org/10.1177/22808000241251564","url":null,"abstract":"<p><strong>Objectives: </strong>This study aims to investigate the effect of coating time on the formation of hydroxyapatite (HA) coating layer on ZK60 substrate and understand the biodegradation behavior of the coated alloy for biodegradable implant applications.</p><p><strong>Methods: </strong>Biodegradable ZK60 alloy was coated by HA layer for different times of 0.5, 1, 2, and 4 h by chemical conversion method. After coating, all the coated specimens were used for immersion test in Hanks' solution to understand the effect of coating time on the degradation behavior of the alloy. The degradation rate of the coated alloy was evaluated by Mg<sup>2+</sup> ion quantification and pH change during immersion test. The microstructure of the coating layer was examined by scanning electron microscope (SEM) equipped with an energy-dispersive X-ray spectroscopy (EDS) before and after immersion to understand the degradation behavior of the coated alloy.</p><p><strong>Results: </strong>HA coating layers were formed successfully on surface of ZK60 specimens after 0.5, 1, 2, and 4 h with different microstructure. Optimal coating quality was observed at 1 or 2 h, characterized by well-formed and uniform HA layers. However, extending the coating duration to 4 h led to the formation of cracks within the HA layer, accompanied by Mg(OH)<sub>2</sub>. Specimens coated for 1 and 2 h exhibited the lowest degradation rates, while specimens coated for 0.5 and 4 h showed the highest degradation rates. Furthermore, analysis of degradation products revealed the predominance of calcium phosphates formed on the surface of specimens coated for 1 and 2 h. Conversely, specimens coated for 0.5 and 4 h exhibited Mg(OH)<sub>2</sub> as the primary degradation product, suggesting a less effective corrosion barrier under these conditions.</p><p><strong>Conclusion: </strong>The HA layer formed after 2 h demonstrated as the most effective coating layer for enhancing the corrosion resistance of the ZK60 alloy for biomedical applications.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141442775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Atomization is a treatment method to make inhaled liquids into aerosols and transport them to target organs in the form of fog or smoke. It has the advantages of improving the bioavailability of drugs, being painless, and non-invasive, and is now widely used in the treatment of lung and oral lesions. Aerosol inhalation as the route of administration of therapeutic proteins holds significant promise due to its ability to achieve high bioavailability in non-invasive pathways. Currently, a great number of therapeutic proteins such as alpha-1 antitrypsin and Dornase alfa are effective. Recombinant humanized collagen type III (rhCol III) as a therapeutic protein is widely used in the biomedical field, but atomization is not a common route of administration for rhCol III, presenting great potential for development. However, the structural stability of recombinant humanized collagen after atomization needs further investigation. This study demonstrated that the rhCol III subjected to atomization through compressed air had retained its original molecular weights, triple helical structures, and the ability to promote cell adhesion. In other words, the rhCol III can maintain its stability after undergoing atomization. Although more research is required to determine the efficacy and safety of the rhCol III after atomization, this study can lay the groundwork for future research.
雾化是一种将吸入的液体制成气溶胶,并以雾或烟的形式输送到靶器官的治疗方法。它具有提高药物生物利用度、无痛、无创伤等优点,目前已广泛应用于肺部和口腔病变的治疗。气溶胶吸入作为治疗蛋白质的给药途径,因其能在非侵入性途径中实现高生物利用度而大有可为。目前,α-1 抗胰蛋白酶和 Dornase alfa 等大量治疗蛋白质都很有效。重组人源化 III 型胶原蛋白(rhCol III)作为一种治疗蛋白在生物医学领域得到了广泛应用,但雾化给药并不是 rhCol III 的常用给药途径,因此具有很大的发展潜力。然而,重组人源化胶原蛋白雾化后的结构稳定性有待进一步研究。本研究表明,通过压缩空气雾化的 rhCol III 保持了原有的分子量、三重螺旋结构和促进细胞粘附的能力。换句话说,rhCol III 在经过雾化后仍能保持其稳定性。虽然还需要更多的研究来确定雾化后 rhCol III 的有效性和安全性,但本研究可为今后的研究奠定基础。
{"title":"Effect of atomization on the composition and structure of recombinant humanized collagen type III.","authors":"Ningwen Cheng, Xinyue Zhang, Jian Wang, Danfeng Li, Ling Li, Huan Hu, Tingli Qu","doi":"10.1177/22808000241261904","DOIUrl":"10.1177/22808000241261904","url":null,"abstract":"<p><p>Atomization is a treatment method to make inhaled liquids into aerosols and transport them to target organs in the form of fog or smoke. It has the advantages of improving the bioavailability of drugs, being painless, and non-invasive, and is now widely used in the treatment of lung and oral lesions. Aerosol inhalation as the route of administration of therapeutic proteins holds significant promise due to its ability to achieve high bioavailability in non-invasive pathways. Currently, a great number of therapeutic proteins such as alpha-1 antitrypsin and Dornase alfa are effective. Recombinant humanized collagen type III (rhCol III) as a therapeutic protein is widely used in the biomedical field, but atomization is not a common route of administration for rhCol III, presenting great potential for development. However, the structural stability of recombinant humanized collagen after atomization needs further investigation. This study demonstrated that the rhCol III subjected to atomization through compressed air had retained its original molecular weights, triple helical structures, and the ability to promote cell adhesion. In other words, the rhCol III can maintain its stability after undergoing atomization. Although more research is required to determine the efficacy and safety of the rhCol III after atomization, this study can lay the groundwork for future research.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141440495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1177/22808000241271693
Anh Tuyet Thi Ngo, Linh Do Chi, Hanh Hong Pham, San Thy Pham, Luong Van Duong
Objectives: The primary objective of this study is using an anodizing intermediate layer to improve corrosion resistance and adhesion of hydroxyapatite coated AZ31 alloy for applications in biodegradable implants.
Methods: An anodizing intermediate layer was formed on the surface of AZ31 substrate at various anodizing voltage of 10, 20, 30, and 40 V respectively by anodizing process. HAp was grow on the surface of AZ31 substrate at 90°C and pH solution of 7.5 by chemical solution treatment method for 2 h. The coated samples were evaluated their corrosion behavior by Electrochemical measurements and biodegradation behavior by immersion test in Hank's balanced salts solution (HBSS) for 28 days via amount of Mg2+ ion released. While, their adhesion strength were evaluated by pull-off method. The amount of Mg2+ ions released of the samples was quantified by the Inductively coupled plasma mass spectrometry.
Results: An anodizing intermediate layer was successfully synthesized at various voltages by anodizing process and HAp coatings were prepared by chemical solution treatment method. The corrosion rate of hydroxyapatite coated AZ31 alloy with an anodizing intermediate layer decreased 4.4 times, while adhesion strength increased about two times compared to the HAp coated AZ31 specimen without an anodizing layer and achieved ~14.70, ~6.92 MPa, respectively. After immersion test in HBSS, the adhesion strength of HAp/AZ31-HBSS-specimen decrease to 45% because of large corroded areas with depth holes of hundreds of micrometers. The slighter decrease in adhesion strength of HAp/30V/AZ31-HBSS-specimen to 22% is due to the contribution of the anodizing intermediate layer.
Conclusion: HAp coated AZ31 alloy specimen with the existence of a porous structure with an elliptical shape, uniform and high density of MgO on the surface at anodizing voltage of 30 V resulted in a significant increase in corrosion resistance and the adhesion strength of HAp coatings.
{"title":"Improvement of corrosion resistance and adhesion of hydroxyapatite coating on AZ31 alloy by an anodizing intermediate layer.","authors":"Anh Tuyet Thi Ngo, Linh Do Chi, Hanh Hong Pham, San Thy Pham, Luong Van Duong","doi":"10.1177/22808000241271693","DOIUrl":"https://doi.org/10.1177/22808000241271693","url":null,"abstract":"<p><strong>Objectives: </strong>The primary objective of this study is using an anodizing intermediate layer to improve corrosion resistance and adhesion of hydroxyapatite coated AZ31 alloy for applications in biodegradable implants.</p><p><strong>Methods: </strong>An anodizing intermediate layer was formed on the surface of AZ31 substrate at various anodizing voltage of 10, 20, 30, and 40 V respectively by anodizing process. HAp was grow on the surface of AZ31 substrate at 90°C and pH solution of 7.5 by chemical solution treatment method for 2 h. The coated samples were evaluated their corrosion behavior by Electrochemical measurements and biodegradation behavior by immersion test in Hank's balanced salts solution (HBSS) for 28 days via amount of Mg<sup>2+</sup> ion released. While, their adhesion strength were evaluated by pull-off method. The amount of Mg<sup>2+</sup> ions released of the samples was quantified by the Inductively coupled plasma mass spectrometry.</p><p><strong>Results: </strong>An anodizing intermediate layer was successfully synthesized at various voltages by anodizing process and HAp coatings were prepared by chemical solution treatment method. The corrosion rate of hydroxyapatite coated AZ31 alloy with an anodizing intermediate layer decreased 4.4 times, while adhesion strength increased about two times compared to the HAp coated AZ31 specimen without an anodizing layer and achieved ~14.70, ~6.92 MPa, respectively. After immersion test in HBSS, the adhesion strength of HAp/AZ31-HBSS-specimen decrease to 45% because of large corroded areas with depth holes of hundreds of micrometers. The slighter decrease in adhesion strength of HAp/30V/AZ31-HBSS-specimen to 22% is due to the contribution of the anodizing intermediate layer.</p><p><strong>Conclusion: </strong>HAp coated AZ31 alloy specimen with the existence of a porous structure with an elliptical shape, uniform and high density of MgO on the surface at anodizing voltage of 30 V resulted in a significant increase in corrosion resistance and the adhesion strength of HAp coatings.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1177/22808000241235442
Ashraf Alemi, Mojtaba Haghi Karamallah, Mohamad Sabaghan, Seyed Ahmad Hosseini, Ali Veisi, Somayeh Haghi Karamallah, Mohammad Farokhifar
Given the numerous adverse effects of lung cancer treatment, more research on non-toxic medications is urgently needed. Curcumin (CUR) and berberine (BBR) combat drug resistance by controlling the expression of multidrug resistant pump (MDR1). Fascinatingly, combining these medications increases the effectiveness of preventing lung cancer. Their low solubility and poor stability, however, restrict their therapeutic efficacy. Because of the improved bioavailability and increased encapsulation effectiveness of water-insoluble medicines, surfactant-based nanovesicles have recently received a great deal of attention. The current study sought to elucidate the Combination drug therapy by herbal nanomedicine prevent multidrug resistance protein 1: promote apoptosis in Lung Carcinoma. The impact of several tween (20, 60, and 80) types with varied hydrophobic tails on BBR/CUR-TNV was evaluated. Additionally, the MDR1 activity and apoptosis rate of the BBR/CUR-TNV combination therapy were assessed. The encapsulation effectiveness of TNV was affected by the type of tween. With the TNV made from tween 60, cholesterol, and PEG (47.5: 47.5:5), more encapsulation effectiveness was attained. By combining CUR with BBR, especially when given in TNV, apoptosis increased. Additionally, when CUR and BBR were administered in combination, they significantly reduced the risk of MDR1 development. The current work suggests that the delivery of berberine and curcumin as a combination medication therapy via tween-based nanovesicles may be a potential lung cancer treatment.
{"title":"Combination drug therapy by herbal nanomedicine prevent multidrug resistance protein 1: promote apoptosis in Lung Carcinoma.","authors":"Ashraf Alemi, Mojtaba Haghi Karamallah, Mohamad Sabaghan, Seyed Ahmad Hosseini, Ali Veisi, Somayeh Haghi Karamallah, Mohammad Farokhifar","doi":"10.1177/22808000241235442","DOIUrl":"10.1177/22808000241235442","url":null,"abstract":"<p><p>Given the numerous adverse effects of lung cancer treatment, more research on non-toxic medications is urgently needed. Curcumin (CUR) and berberine (BBR) combat drug resistance by controlling the expression of multidrug resistant pump (MDR1). Fascinatingly, combining these medications increases the effectiveness of preventing lung cancer. Their low solubility and poor stability, however, restrict their therapeutic efficacy. Because of the improved bioavailability and increased encapsulation effectiveness of water-insoluble medicines, surfactant-based nanovesicles have recently received a great deal of attention. The current study sought to elucidate the Combination drug therapy by herbal nanomedicine prevent multidrug resistance protein 1: promote apoptosis in Lung Carcinoma. The impact of several tween (20, 60, and 80) types with varied hydrophobic tails on BBR/CUR-TNV was evaluated. Additionally, the MDR1 activity and apoptosis rate of the BBR/CUR-TNV combination therapy were assessed. The encapsulation effectiveness of TNV was affected by the type of tween. With the TNV made from tween 60, cholesterol, and PEG (47.5: 47.5:5), more encapsulation effectiveness was attained. By combining CUR with BBR, especially when given in TNV, apoptosis increased. Additionally, when CUR and BBR were administered in combination, they significantly reduced the risk of MDR1 development. The current work suggests that the delivery of berberine and curcumin as a combination medication therapy via tween-based nanovesicles may be a potential lung cancer treatment.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1177/22808000241226656
Sarah Strauß, Maximilian Diemer, Vesna Bucan, Jörn W Kuhbier, Tomke Asendorf, Peter M Vogt, Frederik Schlottmann
Human cartilage tissue remains a challenge for the development of therapeutic options due to its poor vascularization and reduced regenerative capacities. There are a variety of research approaches dealing with cartilage tissue engineering. In addition to different biomaterials, numerous cell populations have been investigated in bioreactor-supported experimental setups to improve cartilage tissue engineering. The concept of the present study was to investigate spider silk cocoons as scaffold seeded with adipose-derived stromal cells (ASC) in a custom-made bioreactor model using cyclic axial compression to engineer cartilage-like tissue. For chemical induction of differentiation, BMP-7 and TGF-β2 were added and changes in cell morphology and de-novo tissue formation were investigated using histological staining to verify chondrogenic differentiation. By seeding spider silk cocoons with ASC, a high colonization density and cell proliferation could be achieved. Mechanical induction of differentiation using a newly established bioreactor model led to a more roundish cell phenotype and new extracellular matrix formation, indicating a chondrogenic differentiation. The addition of BMP-7 and TGF-β2 enhanced the expression of cartilage specific markers in immunohistochemical staining. Overall, the present study can be seen as pilot study and valuable complementation to the published literature.
{"title":"Spider silk enhanced tissue engineering of cartilage tissue: Approach of a novel bioreactor model using adipose derived stromal cells.","authors":"Sarah Strauß, Maximilian Diemer, Vesna Bucan, Jörn W Kuhbier, Tomke Asendorf, Peter M Vogt, Frederik Schlottmann","doi":"10.1177/22808000241226656","DOIUrl":"10.1177/22808000241226656","url":null,"abstract":"<p><p>Human cartilage tissue remains a challenge for the development of therapeutic options due to its poor vascularization and reduced regenerative capacities. There are a variety of research approaches dealing with cartilage tissue engineering. In addition to different biomaterials, numerous cell populations have been investigated in bioreactor-supported experimental setups to improve cartilage tissue engineering. The concept of the present study was to investigate spider silk cocoons as scaffold seeded with adipose-derived stromal cells (ASC) in a custom-made bioreactor model using cyclic axial compression to engineer cartilage-like tissue. For chemical induction of differentiation, BMP-7 and TGF-β2 were added and changes in cell morphology and de-novo tissue formation were investigated using histological staining to verify chondrogenic differentiation. By seeding spider silk cocoons with ASC, a high colonization density and cell proliferation could be achieved. Mechanical induction of differentiation using a newly established bioreactor model led to a more roundish cell phenotype and new extracellular matrix formation, indicating a chondrogenic differentiation. The addition of BMP-7 and TGF-β2 enhanced the expression of cartilage specific markers in immunohistochemical staining. Overall, the present study can be seen as pilot study and valuable complementation to the published literature.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139521011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1177/22808000241277383
Ivan Vito Ferrari, Micaela Castellino, Anissa Pisani, Giulia Giuntoli, Aida Cavallo, Tamer Al Kayal, Paola Mazzetti, Alfredo Rosellini, Maria Sidoti, Antonino Cataldo, Mauro Pistello, Giorgio Soldani, Paola Losi
In the past few years, due to the Covid-19 pandemic, the interest towards textiles with antimicrobial functionalities faced a significant boost. This study proposes a rapid and convenient method, in terms of reactants and equipment, for fabricating antimicrobial coatings on textiles. Through the electroless silver plating reaction, silver coatings were successfully applied on cotton and polyester, rapidly and at room temperature. Functionalized samples were characterized by morphological (optical and scanning electron microscopies) and chemical tests (X-ray photoelectron spectroscopy, XPS) to investigate the nature of the silver coating. Although distinct nanoparticles did not form, XPS analysis detected the presence of silver, which resulted in an increased surface roughness and hydrophobicity of both cotton and polyester textiles. Ag-coated samples exhibited approximately 80% biocompatibility with murine L929 fibroblasts or human HaCaT cells, and strong antibacterial properties against Escherichia coli in direct contact tests. In antiviral experiments with SARS-CoV-2 virus, treated cotton showed a 100% viral reduction in 30 min, while polyester achieved 100% reduction in 1 h. With a human norovirus surrogate, the Feline Calicivirus, both treated textiles have a faster antiviral response, with more than 60% viral reduction after 5 min, while achieving a 100% reduction in 1 h. In conclusion, this study presents a fast, efficient, and low-cost solution for producing antimicrobial textiles with broad applications in medical and healthcare scenarios.
{"title":"Electroless silver plating on fabrics for antimicrobial coating: comparison between cotton and polyester.","authors":"Ivan Vito Ferrari, Micaela Castellino, Anissa Pisani, Giulia Giuntoli, Aida Cavallo, Tamer Al Kayal, Paola Mazzetti, Alfredo Rosellini, Maria Sidoti, Antonino Cataldo, Mauro Pistello, Giorgio Soldani, Paola Losi","doi":"10.1177/22808000241277383","DOIUrl":"10.1177/22808000241277383","url":null,"abstract":"<p><p>In the past few years, due to the Covid-19 pandemic, the interest towards textiles with antimicrobial functionalities faced a significant boost. This study proposes a rapid and convenient method, in terms of reactants and equipment, for fabricating antimicrobial coatings on textiles. Through the electroless silver plating reaction, silver coatings were successfully applied on cotton and polyester, rapidly and at room temperature. Functionalized samples were characterized by morphological (optical and scanning electron microscopies) and chemical tests (X-ray photoelectron spectroscopy, XPS) to investigate the nature of the silver coating. Although distinct nanoparticles did not form, XPS analysis detected the presence of silver, which resulted in an increased surface roughness and hydrophobicity of both cotton and polyester textiles. Ag-coated samples exhibited approximately 80% biocompatibility with murine L929 fibroblasts or human HaCaT cells, and strong antibacterial properties against <i>Escherichia coli</i> in direct contact tests. In antiviral experiments with SARS-CoV-2 virus, treated cotton showed a 100% viral reduction in 30 min, while polyester achieved 100% reduction in 1 h. With a human norovirus surrogate, the Feline Calicivirus, both treated textiles have a faster antiviral response, with more than 60% viral reduction after 5 min, while achieving a 100% reduction in 1 h. In conclusion, this study presents a fast, efficient, and low-cost solution for producing antimicrobial textiles with broad applications in medical and healthcare scenarios.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1177/22808000241240296
Rema A Oliver, Tian Wang, Chris Christou, Alisa Buchman, Simha Sibony, William R Walsh
Joint replacements provide pain free movement for the injured or our aging population. Current prothesis mainly consist of hard metal on metal, or ceramic femoral head on ultra-high-molecular weight polyethylene (UHMWPE). In this study, a rodent fracture model was used to test the influence of wear debris from a high-performance polymer (polyimide MP-1™). Saline, MP-1™ Low Dose in Saline (1%), or MP-1 High Dose (2%) in Saline was injected directly into a standard closed unilateral femoral fracture in 12-week old Sprague Dawley rats (n = 25) for 1, 3 and 6 weeks. Endpoints included radiography, micro-computed tomography, mechanical testing and paraffin histology. No adverse effects from the wear particles were observed from the current study based on radiology, mechanical or histological data. Although the particles were present, histological analysis revealed a progression in healing between the Polyimide treated groups and the non-treated saline control groups over the duration of 1, 3, and 6 weeks, with no inhibition from the particles. The MP-1™ wear debris generated are larger than 1 µm thus are not able to be engulfed by macrophages and cause osteolysis. This family of polymers (polyimides) may be an ideal material to consider for articulating joints and other implants in the human body.
{"title":"The influence of polyimide MP-1™ wear particles on a rodent closed fracture healing model.","authors":"Rema A Oliver, Tian Wang, Chris Christou, Alisa Buchman, Simha Sibony, William R Walsh","doi":"10.1177/22808000241240296","DOIUrl":"10.1177/22808000241240296","url":null,"abstract":"<p><p>Joint replacements provide pain free movement for the injured or our aging population. Current prothesis mainly consist of hard metal on metal, or ceramic femoral head on ultra-high-molecular weight polyethylene (UHMWPE). In this study, a rodent fracture model was used to test the influence of wear debris from a high-performance polymer (polyimide MP-1™). Saline, MP-1™ Low Dose in Saline (1%), or MP-1 High Dose (2%) in Saline was injected directly into a standard closed unilateral femoral fracture in 12-week old Sprague Dawley rats (<i>n</i> = 25) for 1, 3 and 6 weeks. Endpoints included radiography, micro-computed tomography, mechanical testing and paraffin histology. No adverse effects from the wear particles were observed from the current study based on radiology, mechanical or histological data. Although the particles were present, histological analysis revealed a progression in healing between the Polyimide treated groups and the non-treated saline control groups over the duration of 1, 3, and 6 weeks, with no inhibition from the particles. The MP-1™ wear debris generated are larger than 1 µm thus are not able to be engulfed by macrophages and cause osteolysis. This family of polymers (polyimides) may be an ideal material to consider for articulating joints and other implants in the human body.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140174841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.1177/22808000241266665
Wufanbieke Baheti, Xiaotao Chen, Mi La, Huiyu He
The pro-inflammatory/anti-inflammatory polarized phenotypes of macrophages (M1/M2) can be used to predict the success of implant integration. Hence, activating and inducing the transformation of immunocytes that promote tissue repair appears to be a highly promising strategy for facilitating osteo-anagenesis. In a previous study, titanium implants were coated with a graphene oxide-hydroxyapatite (GO-HA) nanocomposite via electrophoretic deposition, and the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was found to be significantly enhanced when the GO content was 2wt%. However, the effectiveness of the GO-HA nanocomposite coating in modifying the in vivo immune microenvironment still remains unclear. In this study, the effects of GO-HA coatings on osteogenesis were investigated based on the GO-HA-mediated immune regulation of macrophages. The HA-2wt%GO nanocomposite coatings exhibited good biocompatibility and favored M2 macrophage polarization. Meanwhile, they could also significantly upregulate IL-10 (anti-inflammatory factor) expression and downregulate TNF-α (pro-inflammatory factor) expression. Additionally, the microenvironment, which was established by M2 macrophages, favored the osteogenesis of BMSCs both in vivo and in vitro. These findings show that the GO-HA nanocomposite coating is a promising surface-modification material. Hence, this study provides a reference for the development of next-generation osteoimmunomodulatory biomaterials.
{"title":"Biomimetic HA-GO implant coating for enhanced osseointegration via macrophage M2 polarization-induced osteo-immunomodulation.","authors":"Wufanbieke Baheti, Xiaotao Chen, Mi La, Huiyu He","doi":"10.1177/22808000241266665","DOIUrl":"https://doi.org/10.1177/22808000241266665","url":null,"abstract":"<p><p>The pro-inflammatory/anti-inflammatory polarized phenotypes of macrophages (M1/M2) can be used to predict the success of implant integration. Hence, activating and inducing the transformation of immunocytes that promote tissue repair appears to be a highly promising strategy for facilitating osteo-anagenesis. In a previous study, titanium implants were coated with a graphene oxide-hydroxyapatite (GO-HA) nanocomposite via electrophoretic deposition, and the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) was found to be significantly enhanced when the GO content was 2wt%. However, the effectiveness of the GO-HA nanocomposite coating in modifying the in vivo immune microenvironment still remains unclear. In this study, the effects of GO-HA coatings on osteogenesis were investigated based on the GO-HA-mediated immune regulation of macrophages. The HA-2wt%GO nanocomposite coatings exhibited good biocompatibility and favored M2 macrophage polarization. Meanwhile, they could also significantly upregulate IL-10 (anti-inflammatory factor) expression and downregulate TNF-α (pro-inflammatory factor) expression. Additionally, the microenvironment, which was established by M2 macrophages, favored the osteogenesis of BMSCs both in vivo and in vitro. These findings show that the GO-HA nanocomposite coating is a promising surface-modification material. Hence, this study provides a reference for the development of next-generation osteoimmunomodulatory biomaterials.</p>","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}