Pub Date : 2023-07-09DOI: 10.18499/2225-7357-2023-12-2-106-110
A. G. Alekseev, A. V. Maksimov, N. P. Chizhikov, A. A. Poletaeva, D. A. Sokolov, A. A. Shevchenko
Developmental anomalies of the aortic arch and its large vessels can be accompanied not only by hemodynamic disorders, but also affect the functions of internal organs. This report analyzes the case of an autopsy finding of the right aberrant subclavian artery in an 80-year-old patient, which was clinically manifested by the development of dysphagia and cachexia. An autopsy revealed the absence of the brachiocephalic trunk, which normally originates from the arch of the authors, as well as an abnormal origin of the right subclavian artery from the posterior surface of the aortic arch, 1,1 cm to the left and distal to the orifice of the left subclavian artery. The proximal segment of the abnormal vessel, up to 1,0 cm in diameter, was located between the spinal column and the esophagus, leading to its compression.
{"title":"A case of aberrant right subclavian artery with the development of Bayford–Autenrieth dysphagia","authors":"A. G. Alekseev, A. V. Maksimov, N. P. Chizhikov, A. A. Poletaeva, D. A. Sokolov, A. A. Shevchenko","doi":"10.18499/2225-7357-2023-12-2-106-110","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-2-106-110","url":null,"abstract":" Developmental anomalies of the aortic arch and its large vessels can be accompanied not only by hemodynamic disorders, but also affect the functions of internal organs. This report analyzes the case of an autopsy finding of the right aberrant subclavian artery in an 80-year-old patient, which was clinically manifested by the development of dysphagia and cachexia. An autopsy revealed the absence of the brachiocephalic trunk, which normally originates from the arch of the authors, as well as an abnormal origin of the right subclavian artery from the posterior surface of the aortic arch, 1,1 cm to the left and distal to the orifice of the left subclavian artery. The proximal segment of the abnormal vessel, up to 1,0 cm in diameter, was located between the spinal column and the esophagus, leading to its compression.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82603823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-09DOI: 10.18499/2225-7357-2023-12-2-39-48
A. Lebedeva, E. M. Gareev, I. Sirotkina, M. F. Galautdinov
Exhausting physical activity leads to sarcomere overstrain, destruction of the cell membrane, hydrolysis of structural proteins, thus, resulting in irreversible damage to muscle fibers. Allogeneic biomaterial (AB) is applied to regenerate various tissues and organs. The aim of the study was to identify morphofunctional features of the skeletal muscle tissue after physical exertion and under AB application. Material and methods. This experimental study involved male Wistar rats. Anaerobic physical exertion was simulated using the Porsolt test with a load equal 10 % of the body weight for 30 days. After training, animals of the main group (n = 10) were injected AB suspension: 4 ml of a 0.2 % solution into the muscles of the fore and hind limbs totally. Animals of the control group (n = 10) received physiological saline into similar zones. Tolerant load was investigated in 5 and 21 days after injection. Then the animals were withdrawn from the experiment, and muscles of the hind limbs were studied histologically, the total number, the average cross-sectional area of muscle fibers, and the number of necrotic fibers were measured. Results. In 5 and 21 days animals from the control group manifested dystrophic changes in muscle fibers: type III, IV contractures, microcirculation disorders, mosaic necrosis of muscle fibers, inflammatory cell infiltration, and a decreased tolerant load. In 21 days fibrosis was detected. The AB introduction provided rhabdomyogenesis as soon as in 5 days. Inflammatory cell infiltration decreased, the polygonality of muscle fiber profiles was restored, and edematous phenomena were leveled. There was hyperplasia of muscle fibers, a decreased number of necrotic muscle fibers, inhibited fibrosis, and an increased tolerant load. AB further biodegraded. Conclusion. AB contributed to the reduced manifestations of dystrophic changes in muscle fibers, strengthened actoprotective mechanism, and restored physical activity in the early stages.
体力活动过度导致肌节过度劳损、细胞膜破坏、结构蛋白水解,从而对肌纤维造成不可逆的损伤。同种异体生物材料(AB)被应用于多种组织和器官的再生。本研究的目的是确定体力消耗和AB应用后骨骼肌组织的形态功能特征。材料和方法。本实验研究对象为雄性Wistar大鼠。采用Porsolt试验模拟无氧体力消耗,负荷等于体重的10%,持续30天。训练结束后,主组(n = 10)将AB悬浮液:4 ml 0.2%溶液全部注射到前肢和后肢肌肉中。对照组(n = 10)在相同区域内注射生理盐水。在注射后5天和21天观察耐受负荷。然后退出实验,对大鼠后肢肌肉进行组织学研究,测定肌纤维总数、平均截面积和坏死纤维数量。结果。在第5天和第21天,对照组动物表现出肌纤维营养不良的变化:III型、IV型挛缩、微循环障碍、肌纤维花叶性坏死、炎症细胞浸润和耐受负荷下降。21 d检测到纤维化。AB的引入在5天内就能产生横纹肌。炎性细胞浸润减少,肌纤维多角性恢复,水肿现象趋于平缓。肌纤维增生,坏死肌纤维数量减少,纤维化受到抑制,耐受负荷增加。AB进一步生物降解。结论。AB有助于减少肌纤维营养不良变化的表现,增强肌动保护机制,恢复早期体力活动。
{"title":"Morphological and functional changes in the skeletal muscles of the hind limbs in rats under enforced anaerobic physical exertion and allogeneic biomaterial application","authors":"A. Lebedeva, E. M. Gareev, I. Sirotkina, M. F. Galautdinov","doi":"10.18499/2225-7357-2023-12-2-39-48","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-2-39-48","url":null,"abstract":" Exhausting physical activity leads to sarcomere overstrain, destruction of the cell membrane, hydrolysis of structural proteins, thus, resulting in irreversible damage to muscle fibers. Allogeneic biomaterial (AB) is applied to regenerate various tissues and organs. The aim of the study was to identify morphofunctional features of the skeletal muscle tissue after physical exertion and under AB application. Material and methods. This experimental study involved male Wistar rats. Anaerobic physical exertion was simulated using the Porsolt test with a load equal 10 % of the body weight for 30 days. After training, animals of the main group (n = 10) were injected AB suspension: 4 ml of a 0.2 % solution into the muscles of the fore and hind limbs totally. Animals of the control group (n = 10) received physiological saline into similar zones. Tolerant load was investigated in 5 and 21 days after injection. Then the animals were withdrawn from the experiment, and muscles of the hind limbs were studied histologically, the total number, the average cross-sectional area of muscle fibers, and the number of necrotic fibers were measured. Results. In 5 and 21 days animals from the control group manifested dystrophic changes in muscle fibers: type III, IV contractures, microcirculation disorders, mosaic necrosis of muscle fibers, inflammatory cell infiltration, and a decreased tolerant load. In 21 days fibrosis was detected. The AB introduction provided rhabdomyogenesis as soon as in 5 days. Inflammatory cell infiltration decreased, the polygonality of muscle fiber profiles was restored, and edematous phenomena were leveled. There was hyperplasia of muscle fibers, a decreased number of necrotic muscle fibers, inhibited fibrosis, and an increased tolerant load. AB further biodegraded. Conclusion. AB contributed to the reduced manifestations of dystrophic changes in muscle fibers, strengthened actoprotective mechanism, and restored physical activity in the early stages.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"24 10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91051863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-09DOI: 10.18499/2225-7357-2023-12-2-99-105
A. E. Kasparova, E. D. Khadieva, E. I. Kutefa, V. L. Yanin, E. N. Vasil'kovskaya, L. Chegus, N. A. Sazonova, F. R. Khidirnebieva
Since the beginning of the COVID-19 pandemic, there have been a number of studies related to the impact of SARS-CoV-2 on the course of pregnancy and fetus condition. As observed, the placentas of women who have had a new coronavirus infection often contain more villous agglutination and subchorionic thrombi than placentas in SARS-CoV-2-negative women. To date, several isolated publications have reported clinical cases of fetal death in mothers infected with coronavirus infection. The authors have made an assumption on the association of adverse outcomes with placental lesions. The aim of the study was to analyse a clinical case of a moderate-course new coronavirus infection in a pregnant woman at a long gestation period who underwent an antenatal fetal death, and evaluate the features of placental histopathology and their impact on adverse gestational outcomes. Material and methods. The authors have analysed Russian and international research publications from various sources, including eLIBRARY.RU, CyberLeninka, PubMed databases etc. and, considering the data obtained, investigated a clinical case of intrauterine fetal death in a pregnant woman infected with SARS-CoV-2. The placenta was studied in accordance with the clinical recommendations of the Russian Society of Pathologists "Rules for placental pathological and anatomical examination" and the international classification of placental lesions (Amsterdam, 2015). Results. The results obtained support the association between acute diseases of the upper respiratory tract and developing severe hemodynamic disorders in the "mother-placenta-fetus" system in pregnant women infected with SARS-CoV-2. Systemic inflammation associated with new coronavirus infection appears to be one of the mechanisms for developing placental disorders.
{"title":"An uncommon case of placental histopathology with antenatal fetal death under new coronavirus infection","authors":"A. E. Kasparova, E. D. Khadieva, E. I. Kutefa, V. L. Yanin, E. N. Vasil'kovskaya, L. Chegus, N. A. Sazonova, F. R. Khidirnebieva","doi":"10.18499/2225-7357-2023-12-2-99-105","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-2-99-105","url":null,"abstract":" Since the beginning of the COVID-19 pandemic, there have been a number of studies related to the impact of SARS-CoV-2 on the course of pregnancy and fetus condition. As observed, the placentas of women who have had a new coronavirus infection often contain more villous agglutination and subchorionic thrombi than placentas in SARS-CoV-2-negative women. To date, several isolated publications have reported clinical cases of fetal death in mothers infected with coronavirus infection. The authors have made an assumption on the association of adverse outcomes with placental lesions. The aim of the study was to analyse a clinical case of a moderate-course new coronavirus infection in a pregnant woman at a long gestation period who underwent an antenatal fetal death, and evaluate the features of placental histopathology and their impact on adverse gestational outcomes. Material and methods. The authors have analysed Russian and international research publications from various sources, including eLIBRARY.RU, CyberLeninka, PubMed databases etc. and, considering the data obtained, investigated a clinical case of intrauterine fetal death in a pregnant woman infected with SARS-CoV-2. The placenta was studied in accordance with the clinical recommendations of the Russian Society of Pathologists \"Rules for placental pathological and anatomical examination\" and the international classification of placental lesions (Amsterdam, 2015). Results. The results obtained support the association between acute diseases of the upper respiratory tract and developing severe hemodynamic disorders in the \"mother-placenta-fetus\" system in pregnant women infected with SARS-CoV-2. Systemic inflammation associated with new coronavirus infection appears to be one of the mechanisms for developing placental disorders.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89740506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-09DOI: 10.18499/2225-7357-2023-12-2-79-89
M. Mnikhovich, M. Lozina, I. A. Shiripenko, O. A. Sidorova, T. Bezuglova, A. V. Romanov
This paper presents a literature review evaluating virtopsy options, its development over time and the scope of application nowadays. To analyze the literature sources, we searched for the keyword “virtopsy” in Scopus, Web of Science, Medline, PubMed, and RSCI databases for the period 2006–2022, the results included 199 sources. Of these, 73 publications including original and review papers were selected for review. It is known that diagnosis verification and therapy correction are largely possible only thanks to the work of pathologists. The coincidence of the post-mortem diagnosis with the lifetime diagnosis opens up the possibility to optimize the course of therapy in similar cases. The literature analysis allows us to conclude that the level of imaging accuracy and frequency of successful interpretations of post-mortem radiological examinations have increased significantly, the fact being associated both with an improved quality of X-ray optical and scanning equipment, and formation of a relatively extensive database containing previously obtained findings. Thus, virtopsy is currently a powerful auxiliary tool applicable in post-mortem diagnosis. The combined use of classical autopsy and virtopsy can increase the accuracy of diagnosis, which, together with digital data processing and storage tools, will bring the work of specialists to a new level of interdisciplinary interaction.
本文综述了虚拟解剖方法的研究进展,以及目前的应用范围。为了分析文献来源,我们在Scopus、Web of Science、Medline、PubMed和RSCI数据库中检索关键词“virtopsy”,检索时间为2006-2022年,共收录文献199篇。其中,73份出版物(包括原创和评论论文)被选中进行审查。众所周知,诊断验证和治疗纠正在很大程度上要归功于病理学家的工作。死后诊断与终生诊断的巧合为优化类似病例的治疗过程提供了可能性。通过文献分析,我们可以得出这样的结论:成像精度水平和成功解释死后放射检查的频率显著提高,这与x射线光学和扫描设备质量的提高以及包含先前发现的相对广泛的数据库的形成有关。因此,虚拟解剖目前是一种强有力的辅助工具,适用于死后诊断。经典解剖和虚拟解剖的结合使用可以提高诊断的准确性,再加上数字数据处理和存储工具,将专家的工作提升到跨学科互动的新水平。
{"title":"Non-invasive methods in autopsy in practice of pathologists and forensic experts: from the appearance to the present day","authors":"M. Mnikhovich, M. Lozina, I. A. Shiripenko, O. A. Sidorova, T. Bezuglova, A. V. Romanov","doi":"10.18499/2225-7357-2023-12-2-79-89","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-2-79-89","url":null,"abstract":" This paper presents a literature review evaluating virtopsy options, its development over time and the scope of application nowadays. To analyze the literature sources, we searched for the keyword “virtopsy” in Scopus, Web of Science, Medline, PubMed, and RSCI databases for the period 2006–2022, the results included 199 sources. Of these, 73 publications including original and review papers were selected for review. It is known that diagnosis verification and therapy correction are largely possible only thanks to the work of pathologists. The coincidence of the post-mortem diagnosis with the lifetime diagnosis opens up the possibility to optimize the course of therapy in similar cases. The literature analysis allows us to conclude that the level of imaging accuracy and frequency of successful interpretations of post-mortem radiological examinations have increased significantly, the fact being associated both with an improved quality of X-ray optical and scanning equipment, and formation of a relatively extensive database containing previously obtained findings. Thus, virtopsy is currently a powerful auxiliary tool applicable in post-mortem diagnosis. The combined use of classical autopsy and virtopsy can increase the accuracy of diagnosis, which, together with digital data processing and storage tools, will bring the work of specialists to a new level of interdisciplinary interaction.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81609794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-09DOI: 10.18499/2225-7357-2023-12-2-30-38
E. V. Kazantseva, Yu. A. Andreev, D. Starchik
Due to the prevalence of thyroid diseases among women, there is a need for a detailed study of the morphometric parameters of this endocrine organ in diverse age groups. The aim of the study was to investigate the linear sizes and the thyroid gland shapes using sectional method and intravital sonography in women of mature age residing in St. Petersburg. Material and methods. The ultrasound diagnostics was used to examine thyroid glands in 117 women of mature age. The exclusion criteria were neck surgery, thyroid nodules and endocrine system diseases. All women were divided into age groups according to the scheme of age periodization of human ontogenesis adopted in 1965. There were 42 thyroid glands obtained from the women died at the mature age, whose fatal outcome was not associated with the pathology of the endocrine system or the thyroid gland; they were allocated into a separate group. Results. Significant differences were registered in the thickness of the left lobe of the thyroid gland in women of the first and second adulthood periods; there was also detected a significant predominance of the size and volume of the right lobe over the left one. Cluster analysis, relying on the thickness of the isthmus of the thyroid gland and the symmetry of its lobes, allows differentiating three shapes of the thyroid gland: “butterfly-shaped”, semilunar and indefinite. Conclusion. The asymmetry of the thyroid lobes is a typical feature of this organ of the endocrine system at the studied age periods in mature women. When determining the shape of the thyroid gland, dissecting and morphometry are more informative options than ultrasound examination. To compare the findings on the shape of the thyroid gland obtained by various researchers, it is necessary to standardize the technique and apply a unified classification of the thyroid gland shapes.
{"title":"Thyroid gland parameters of mature women living in Saint-Petersburg","authors":"E. V. Kazantseva, Yu. A. Andreev, D. Starchik","doi":"10.18499/2225-7357-2023-12-2-30-38","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-2-30-38","url":null,"abstract":" Due to the prevalence of thyroid diseases among women, there is a need for a detailed study of the morphometric parameters of this endocrine organ in diverse age groups. The aim of the study was to investigate the linear sizes and the thyroid gland shapes using sectional method and intravital sonography in women of mature age residing in St. Petersburg. Material and methods. The ultrasound diagnostics was used to examine thyroid glands in 117 women of mature age. The exclusion criteria were neck surgery, thyroid nodules and endocrine system diseases. All women were divided into age groups according to the scheme of age periodization of human ontogenesis adopted in 1965. There were 42 thyroid glands obtained from the women died at the mature age, whose fatal outcome was not associated with the pathology of the endocrine system or the thyroid gland; they were allocated into a separate group. Results. Significant differences were registered in the thickness of the left lobe of the thyroid gland in women of the first and second adulthood periods; there was also detected a significant predominance of the size and volume of the right lobe over the left one. Cluster analysis, relying on the thickness of the isthmus of the thyroid gland and the symmetry of its lobes, allows differentiating three shapes of the thyroid gland: “butterfly-shaped”, semilunar and indefinite. Conclusion. The asymmetry of the thyroid lobes is a typical feature of this organ of the endocrine system at the studied age periods in mature women. When determining the shape of the thyroid gland, dissecting and morphometry are more informative options than ultrasound examination. To compare the findings on the shape of the thyroid gland obtained by various researchers, it is necessary to standardize the technique and apply a unified classification of the thyroid gland shapes.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88175257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-09DOI: 10.18499/2225-7357-2023-12-2-57-63
V. A. Prosvetov, I. Gaivoronskii, D. Surov
The study of morphometric parameters of the thoracic diaphragm in diverse body constructions is of applied relevance in thoracic and abdominal surgery. The aim of the study was to investigate regional morphometric parameters of the thoracic diaphragm in diverse body constructions. Material and methods. The study involved 40 specially embalmed corpses (with preserved tissue elasticity), aged 35-75 when dying. To assess the morphometric parameters of the diaphragm, there was tailor-made an organocomplex represented by the abdominal cavity covered by an intact diaphragm. Longitudinal, transverse dimensions, the area of the diaphragm and its parts were investigated. Results. The analysed morphometric features allowed specifying that in extreme body constructions, there were considerable statistically significant differences in the morphometric parameters of the diaphragm and its parts. It was established that the largest area of the diaphragm, central tendon, costal part and its pericardial surface were observed in people with a brachymorphic body construction; the highest statistical significance was observed between extreme body constructions. The ratio of the tendon part to the total area was also the largest in the brachymorphic form, and the smallest in the dolichomorphic form. There were specific morphometric parameters typical of all types of body constructions: the ratio of the muscular and tendon parts of the diaphragm, the depth of the pleural sinuses, and the areas of the weak points of the diaphragm. The largest linear dimensions of all parts of the diaphragm were recorded in individuals with a brachymorphic body construction. On the contrary, people with dolichomorphic body type had the lowest values. However, the diaphragm domes were the highest in individuals with a dolichomorphic form, and the shortest in those with a brachymorphic form. Conclusion. The study of the morphometric parameters of the thoracic diaphragm allowed recording the major linear dimensions and areas of definite parts specific for all body constructions. The results obtained can be introduced into clinical practice to plan surgical interventions on the upper floor of the peritoneal cavity, including diaphragmatic peritonectomy.
{"title":"Morphometric parameters of the thoracic diaphragm in diverse body constructions","authors":"V. A. Prosvetov, I. Gaivoronskii, D. Surov","doi":"10.18499/2225-7357-2023-12-2-57-63","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-2-57-63","url":null,"abstract":" The study of morphometric parameters of the thoracic diaphragm in diverse body constructions is of applied relevance in thoracic and abdominal surgery. The aim of the study was to investigate regional morphometric parameters of the thoracic diaphragm in diverse body constructions. Material and methods. The study involved 40 specially embalmed corpses (with preserved tissue elasticity), aged 35-75 when dying. To assess the morphometric parameters of the diaphragm, there was tailor-made an organocomplex represented by the abdominal cavity covered by an intact diaphragm. Longitudinal, transverse dimensions, the area of the diaphragm and its parts were investigated. Results. The analysed morphometric features allowed specifying that in extreme body constructions, there were considerable statistically significant differences in the morphometric parameters of the diaphragm and its parts. It was established that the largest area of the diaphragm, central tendon, costal part and its pericardial surface were observed in people with a brachymorphic body construction; the highest statistical significance was observed between extreme body constructions. The ratio of the tendon part to the total area was also the largest in the brachymorphic form, and the smallest in the dolichomorphic form. There were specific morphometric parameters typical of all types of body constructions: the ratio of the muscular and tendon parts of the diaphragm, the depth of the pleural sinuses, and the areas of the weak points of the diaphragm. The largest linear dimensions of all parts of the diaphragm were recorded in individuals with a brachymorphic body construction. On the contrary, people with dolichomorphic body type had the lowest values. However, the diaphragm domes were the highest in individuals with a dolichomorphic form, and the shortest in those with a brachymorphic form. Conclusion. The study of the morphometric parameters of the thoracic diaphragm allowed recording the major linear dimensions and areas of definite parts specific for all body constructions. The results obtained can be introduced into clinical practice to plan surgical interventions on the upper floor of the peritoneal cavity, including diaphragmatic peritonectomy.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91074825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-08DOI: 10.18499/2225-7357-2023-12-2-22-29
A. A. Zhukov, N. Alexeeva, V. V. Shishkina, T. V. Samoilenko, E. S. Goryushkina, S. Zolotareva, S. A. Budnevskaya, E. E. Ivanova, S. Klochkova
The effect of altered gravity on the digestive system is one of the challenging issues in space biology and medicine. The study of the effect of weightlessness on the mast cell (MC) functioning in the gastrointestinal organs is determined by their multifunctionality and participation in the developing adaptive and pathological processes. The aim of the study was to investigate the morphofunctional state of MCs in the stomach membranes of Wistar rats during 14 days of ground-based simulated weightlessness conditions and in a 3-day recovery period. Material and methods. The experiment involved 3 groups of Wistar rats: control, experimental – exposed to antiorthostatic suspension – and a 3-day-recovery group, 7 animals each, respectively. Identification of MCs was carried out using May-Grunwald staining with additional staining with Giemsa solution and immunohistochemical tryptase detection. The results obtained were statistically processed with SPSS 13 using parametric and non-parametric criteria. Results. A significantly increased number of metachromatic MC population was found only in the mucosa in animals of the antiorthostatic suspension and 3-day-recovery groups compared to the vivarium control. There was a sharp decrease in degranulating forms of MCs in the submucosalcoat in animals of the 3-day-recovery group compared to the control. A significantly increased number of degranulating forms of MCs was revealed in the muscle membrane in animals of the antiorthostatic suspension group. Immunohistochemical staining of MCs with antibodies to tryptase, in contrast to staining for metachromasia, allowed identifying a significant representation of tryptase-positive cells in the gastric mucosa in animals of the 3-day-recovery group. Conclusion. The results obtained support gravisensitivity of certain structural components of the stomach, and also evidence MC and their secretome participation in the developing adaptive responses to the effects of altered gravity both at the cellular and tissue levels.
{"title":"Gastric mast cell population in rats under ground-simulated weightlessness conditions and in early readaptation period","authors":"A. A. Zhukov, N. Alexeeva, V. V. Shishkina, T. V. Samoilenko, E. S. Goryushkina, S. Zolotareva, S. A. Budnevskaya, E. E. Ivanova, S. Klochkova","doi":"10.18499/2225-7357-2023-12-2-22-29","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-2-22-29","url":null,"abstract":" The effect of altered gravity on the digestive system is one of the challenging issues in space biology and medicine. The study of the effect of weightlessness on the mast cell (MC) functioning in the gastrointestinal organs is determined by their multifunctionality and participation in the developing adaptive and pathological processes. The aim of the study was to investigate the morphofunctional state of MCs in the stomach membranes of Wistar rats during 14 days of ground-based simulated weightlessness conditions and in a 3-day recovery period. Material and methods. The experiment involved 3 groups of Wistar rats: control, experimental – exposed to antiorthostatic suspension – and a 3-day-recovery group, 7 animals each, respectively. Identification of MCs was carried out using May-Grunwald staining with additional staining with Giemsa solution and immunohistochemical tryptase detection. The results obtained were statistically processed with SPSS 13 using parametric and non-parametric criteria. Results. A significantly increased number of metachromatic MC population was found only in the mucosa in animals of the antiorthostatic suspension and 3-day-recovery groups compared to the vivarium control. There was a sharp decrease in degranulating forms of MCs in the submucosalcoat in animals of the 3-day-recovery group compared to the control. A significantly increased number of degranulating forms of MCs was revealed in the muscle membrane in animals of the antiorthostatic suspension group. Immunohistochemical staining of MCs with antibodies to tryptase, in contrast to staining for metachromasia, allowed identifying a significant representation of tryptase-positive cells in the gastric mucosa in animals of the 3-day-recovery group. Conclusion. The results obtained support gravisensitivity of certain structural components of the stomach, and also evidence MC and their secretome participation in the developing adaptive responses to the effects of altered gravity both at the cellular and tissue levels.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82851910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-08DOI: 10.18499/2225-7357-2023-12-2-9-21
V. A. Akulinin, A. Y. Shoronova, S. Stepanov, M. Korzhuk, L. M. Makar'eva, I. G. Tsuskman, D. Avdeev, L. V. Stepanova
The aim of the study was to investigate morphological changes in neurons, glia and synaptic terminals in cytoarchitectonic fields CA1 and CA3 of the hippocampus in white rats after severe traumatic brain injury (STBI). Material and methods. This experimental study involved outbred adult white Wistar rats. STBI (main group, n = 30) was simulated under anesthesia (Zoletil-100) on the tailor-made device. Intact animals (n = 6) were used as controls. Morphological assessment of the hippocampal nervous tissue (in fields CA1 and CA3) was carried out using light microscopy (staining with hematoxylin and eosin, thionin according to Nissl), immunohistochemical reactions for synaptophysin (p38), Caspase 3, GFAP, and morphometric investigation techniques (ImageJ 1.53 program) in animals of the control group and in experimental animals in 1, 3, 7, 14, 30 days after STBI. Statistically hypotheses were tested using nonparametric tests (Kolmogorov–Smirnov, Kruskal–Wallis, Mann–Whitney, Wilcoxon, Spearman tests) in the Statistica program. 10.0. Results. In a day after STBI, there were noted degeneratively altered dark neurons, shadow cells, small foci of neuron prolapse, pronounced manifestations of edema-swelling in the perikarya and neuropil. Coagulative-ischemic mechanisms of neuronal death prevailed, structural and immunohistochemical signs of apoptosis activation (Caspase 3 in the perikarya) were detected in sporadic cells of the field CA1. In the late period of the study (in 30 days), hydropic changes, chromatolysis with cytoplasmic clearing, hyperchromia without and with homogenization of neuronal structures persisted. An increased neuroglial index was distinctive. Statistically significant differences in the main morphometric parameters were revealed between segments and terms. The total number density of neurons decreased by 43.8 % in CA1 and in CA3 by 22.0 % in 30 days after injury; a significant (20–40 %) deficit of neuropil synapse terminals remained. Thus, the compensatory-restorative reorganization of the hippocampal neuronal networks occurred combined with its structural and functional insufficiency. Probably, Caspase 3 was crucial in implementing neuroplasticity. Conclusion. Post-STBI diffuse-focal pathomorphological changes were revealed in CA1 and CA3 cells of the hippocampus. An increased relative content of glial cells indicated the realization of sanogenesis of the nervous tissue. Reorganization of interneuronal connections occurred during 30 days of observation, caspases might be participants of this process.
{"title":"Morphological bases of reorganization of the hippocampal interneuronal relationships in white rats after severe traumatic brain injury","authors":"V. A. Akulinin, A. Y. Shoronova, S. Stepanov, M. Korzhuk, L. M. Makar'eva, I. G. Tsuskman, D. Avdeev, L. V. Stepanova","doi":"10.18499/2225-7357-2023-12-2-9-21","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-2-9-21","url":null,"abstract":" The aim of the study was to investigate morphological changes in neurons, glia and synaptic terminals in cytoarchitectonic fields CA1 and CA3 of the hippocampus in white rats after severe traumatic brain injury (STBI). Material and methods. This experimental study involved outbred adult white Wistar rats. STBI (main group, n = 30) was simulated under anesthesia (Zoletil-100) on the tailor-made device. Intact animals (n = 6) were used as controls. Morphological assessment of the hippocampal nervous tissue (in fields CA1 and CA3) was carried out using light microscopy (staining with hematoxylin and eosin, thionin according to Nissl), immunohistochemical reactions for synaptophysin (p38), Caspase 3, GFAP, and morphometric investigation techniques (ImageJ 1.53 program) in animals of the control group and in experimental animals in 1, 3, 7, 14, 30 days after STBI. Statistically hypotheses were tested using nonparametric tests (Kolmogorov–Smirnov, Kruskal–Wallis, Mann–Whitney, Wilcoxon, Spearman tests) in the Statistica program. 10.0. Results. In a day after STBI, there were noted degeneratively altered dark neurons, shadow cells, small foci of neuron prolapse, pronounced manifestations of edema-swelling in the perikarya and neuropil. Coagulative-ischemic mechanisms of neuronal death prevailed, structural and immunohistochemical signs of apoptosis activation (Caspase 3 in the perikarya) were detected in sporadic cells of the field CA1. In the late period of the study (in 30 days), hydropic changes, chromatolysis with cytoplasmic clearing, hyperchromia without and with homogenization of neuronal structures persisted. An increased neuroglial index was distinctive. Statistically significant differences in the main morphometric parameters were revealed between segments and terms. The total number density of neurons decreased by 43.8 % in CA1 and in CA3 by 22.0 % in 30 days after injury; a significant (20–40 %) deficit of neuropil synapse terminals remained. Thus, the compensatory-restorative reorganization of the hippocampal neuronal networks occurred combined with its structural and functional insufficiency. Probably, Caspase 3 was crucial in implementing neuroplasticity. Conclusion. Post-STBI diffuse-focal pathomorphological changes were revealed in CA1 and CA3 cells of the hippocampus. An increased relative content of glial cells indicated the realization of sanogenesis of the nervous tissue. Reorganization of interneuronal connections occurred during 30 days of observation, caspases might be participants of this process.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78102592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-14DOI: 10.18499/2225-7357-2023-12-1-44-49
A. Lozinski, S. V. Chemezov
The aim of the study was to specify the age and gender features of the ascending and descending colon location in children and adolescents based on computed tomography scans.Material and methods. The study included computed tomography scans of the abdomen of 75 children without visible pathology of the abdominal organs. All human subjects were divided into 4 age groups: the period of early childhood, the period of the first childhood, the period of the second childhood, adolescence. They were exposed to 16- slice computed tomography followed by analysis specifying distances between the ascending and descending colons and the median sagittal plane. The data obtained were subjected to variational statistical processing, the mean value (M), standard error (m) and the significance of differences according to Student's t-test were defined.Results. The results obtained demonstrated that the maximum values of the distances between the ascending colon and the median sagittal plane were determined at the LIII–LV levels in children of the 1st group, at the LV level in children of the 2nd group, at the LIV level in children of the 3rd group and at the LIII–LIV levels in children the 4th group. The minimum values of the studied parameters were found at the LI level in boys of the 1st group and girls of the 2nd group. The minimum value of the parameter was defined at the LII level in the rest of the examined children. The maximum distances between the descending colon and the median sagittal plane were defined at the LI–LII levels in children of the 1st and 2nd groups, and at the LII–LIII levels in children of the 3rd and 4th groups. The minimum value of the parameter was at the LV level in the examined children of groups 1 and 2, and at the LI and LV levels in the examined children of groups 3 and 4.Conclusion. A significant increase in the average distance from the median sagittal plane to the ascending and descending colons was detected in each group compared to the previous one. Significant differences in the distance from the median sagittal plane to the ascending and descending colons among girls and boys of the same age have not been revealed in any of the age groups.
{"title":"Computed Tomographic Anatomy of the Ascending and Descending Colon in Children and Adolescents","authors":"A. Lozinski, S. V. Chemezov","doi":"10.18499/2225-7357-2023-12-1-44-49","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-1-44-49","url":null,"abstract":"The aim of the study was to specify the age and gender features of the ascending and descending colon location in children and adolescents based on computed tomography scans.Material and methods. The study included computed tomography scans of the abdomen of 75 children without visible pathology of the abdominal organs. All human subjects were divided into 4 age groups: the period of early childhood, the period of the first childhood, the period of the second childhood, adolescence. They were exposed to 16- slice computed tomography followed by analysis specifying distances between the ascending and descending colons and the median sagittal plane. The data obtained were subjected to variational statistical processing, the mean value (M), standard error (m) and the significance of differences according to Student's t-test were defined.Results. The results obtained demonstrated that the maximum values of the distances between the ascending colon and the median sagittal plane were determined at the LIII–LV levels in children of the 1st group, at the LV level in children of the 2nd group, at the LIV level in children of the 3rd group and at the LIII–LIV levels in children the 4th group. The minimum values of the studied parameters were found at the LI level in boys of the 1st group and girls of the 2nd group. The minimum value of the parameter was defined at the LII level in the rest of the examined children. The maximum distances between the descending colon and the median sagittal plane were defined at the LI–LII levels in children of the 1st and 2nd groups, and at the LII–LIII levels in children of the 3rd and 4th groups. The minimum value of the parameter was at the LV level in the examined children of groups 1 and 2, and at the LI and LV levels in the examined children of groups 3 and 4.Conclusion. A significant increase in the average distance from the median sagittal plane to the ascending and descending colons was detected in each group compared to the previous one. Significant differences in the distance from the median sagittal plane to the ascending and descending colons among girls and boys of the same age have not been revealed in any of the age groups.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"216 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76081038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-14DOI: 10.18499/2225-7357-2023-12-1-72-78
V. I. Chernyavskii, K. Abduvosidov, E. N. Galeisya, E. A. Kidyaeva
The aim of the study was to investigate morphometric parameters of the parotid gland in various shapes of the facial and brain skull.Material and methods. The study included cadaveric biomaterial obtained from individuals of both sexes aged 48 to 73 years. There were investigated 120 human head organcomplexes containing 240 parotid glands. The samples were used to measure the upper height of the face and its width, the longitudinal and transverse diameters of the skull, and to calculate the values of the upper facial and transverse longitudinal indexes. Then the soft tissues were dissected in layers, and the parotid glands and adjacent neurovascular formations were isolated. Morphometric parameters of the parotid glands were processed statistically.Results. It was found that the vertical size of the parotid gland correlated with the upper facial index, and the sagittal size correlated with the transverse-longitudinal index. Statistically significant relationships between the upper facial, transverse-longitudinal indices and the shape of the parotid gland were not recorded.Conclusion. The study revealed an average positive correlation between the morphometric parameters of the parotid gland and the upper facial and transverse longitudinal indices. The results of the study complement the available data on the anatomy of the parotid gland and can be used in planning surgical interventions, as well as in interpreting the results of diagnostic radiation studies.
{"title":"Morphometric Parameters of the Parotid Salivary Gland in Various Shape of the Skull","authors":"V. I. Chernyavskii, K. Abduvosidov, E. N. Galeisya, E. A. Kidyaeva","doi":"10.18499/2225-7357-2023-12-1-72-78","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-1-72-78","url":null,"abstract":"The aim of the study was to investigate morphometric parameters of the parotid gland in various shapes of the facial and brain skull.Material and methods. The study included cadaveric biomaterial obtained from individuals of both sexes aged 48 to 73 years. There were investigated 120 human head organcomplexes containing 240 parotid glands. The samples were used to measure the upper height of the face and its width, the longitudinal and transverse diameters of the skull, and to calculate the values of the upper facial and transverse longitudinal indexes. Then the soft tissues were dissected in layers, and the parotid glands and adjacent neurovascular formations were isolated. Morphometric parameters of the parotid glands were processed statistically.Results. It was found that the vertical size of the parotid gland correlated with the upper facial index, and the sagittal size correlated with the transverse-longitudinal index. Statistically significant relationships between the upper facial, transverse-longitudinal indices and the shape of the parotid gland were not recorded.Conclusion. The study revealed an average positive correlation between the morphometric parameters of the parotid gland and the upper facial and transverse longitudinal indices. The results of the study complement the available data on the anatomy of the parotid gland and can be used in planning surgical interventions, as well as in interpreting the results of diagnostic radiation studies.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87637873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}