Pub Date : 2024-04-06DOI: 10.18499/2225-7357-2024-13-1-19-27
N. V. Bubnova, G. Struchko, L. M. Merkulova, О. Kostrova, А. А. Kotelkina, М. N. Mikhailova
The aim of the study is to assess the cellular composition of rat thymus when isolated administration of selenium, urethane and combined exposure to two factors. Material and methods. The study was performed on 34 male Wistar rats, which were divided into 4 groups: 1 – intact, 2 – rats, which received selenium only for 1 month, 3 – animals, which were injected once intraperitoneal urethane, 4 – rats with combined selenium and urethane action. Histological preparations of the thymus were prepared according to generally accepted methods and further evaluated by the method of review microscopy, morphometry, immunohistochemical reactions, electron microscopy, statistical processing of data. Results. Ninety days after selenium, the rat thymus structure is slightly different from intact animals. The introduction of urethane leads to the development of excitation involution of the thymus, which is characterized by morphological changes, and manifests itself in a change in the shape of the organ lobes, in the expansion of the intercole gaps and in the fat tissue thereof, hyperplasia of the cortical substance of the thymus by increasing the number of mature lymphocytes, epithelial cells positive to pancytokeratin. At the ultramicroscopic level, the thymus lymphocyte decreases its nuclear-cytoplasmic index, and increases the number of mitochondria that have a light matrix with a small amount of crystal. Apoptotic bodies and a large number of electron-transparent vacuoles are visualized. When combined, the thymus structure differs from the intact group by an enlarged brain substance, but the number of epithelial cells that are positive to pancytokeratin and Ki67+-cells, as well as cells that express apoptosisregulating protein, decreases. Electron microscopy also indicates a large amount of mitochondria, but with welldefined crystals, when combined with selenium and urethane. There are a small number of electron-transparent vacuoles. Conclusion. The obtained data make it possible to draw a conclusion about the positive influence of the exchange rate of selenium on the T-cell link of immunity, at least for this period of study. In the course of the experiment, it was found that, against the background of the development of the adenoma of the lung caused by the introduction of urethane, at the stage of alveolar epithelium dysplasia signs of excitant thymus involution were detected. When selenium and urethane are combined, less pronounced changes are observed in the examined organ than in the isolated administration of urethane.
{"title":"Changes in the Cellular Composition of the Rat Thymus 90 Days After Urethane Injection Against the Background of Selenium Intake","authors":"N. V. Bubnova, G. Struchko, L. M. Merkulova, О. Kostrova, А. А. Kotelkina, М. N. Mikhailova","doi":"10.18499/2225-7357-2024-13-1-19-27","DOIUrl":"https://doi.org/10.18499/2225-7357-2024-13-1-19-27","url":null,"abstract":"The aim of the study is to assess the cellular composition of rat thymus when isolated administration of selenium, urethane and combined exposure to two factors. Material and methods. The study was performed on 34 male Wistar rats, which were divided into 4 groups: 1 – intact, 2 – rats, which received selenium only for 1 month, 3 – animals, which were injected once intraperitoneal urethane, 4 – rats with combined selenium and urethane action. Histological preparations of the thymus were prepared according to generally accepted methods and further evaluated by the method of review microscopy, morphometry, immunohistochemical reactions, electron microscopy, statistical processing of data. Results. Ninety days after selenium, the rat thymus structure is slightly different from intact animals. The introduction of urethane leads to the development of excitation involution of the thymus, which is characterized by morphological changes, and manifests itself in a change in the shape of the organ lobes, in the expansion of the intercole gaps and in the fat tissue thereof, hyperplasia of the cortical substance of the thymus by increasing the number of mature lymphocytes, epithelial cells positive to pancytokeratin. At the ultramicroscopic level, the thymus lymphocyte decreases its nuclear-cytoplasmic index, and increases the number of mitochondria that have a light matrix with a small amount of crystal. Apoptotic bodies and a large number of electron-transparent vacuoles are visualized. When combined, the thymus structure differs from the intact group by an enlarged brain substance, but the number of epithelial cells that are positive to pancytokeratin and Ki67+-cells, as well as cells that express apoptosisregulating protein, decreases. Electron microscopy also indicates a large amount of mitochondria, but with welldefined crystals, when combined with selenium and urethane. There are a small number of electron-transparent vacuoles. Conclusion. The obtained data make it possible to draw a conclusion about the positive influence of the exchange rate of selenium on the T-cell link of immunity, at least for this period of study. In the course of the experiment, it was found that, against the background of the development of the adenoma of the lung caused by the introduction of urethane, at the stage of alveolar epithelium dysplasia signs of excitant thymus involution were detected. When selenium and urethane are combined, less pronounced changes are observed in the examined organ than in the isolated administration of urethane.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"26 114","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140734782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-06DOI: 10.18499/2225-7357-2024-13-1-9-18
V. А. Аkhmatov, Yu. S. Spirina, D. S. Ledneva, I. А. Aptekar', А. А. Markov, А. N. Steblyuk, V. A. Shidin, G. S. Solovyev, A. R. Nurgalieva, О. G. Solovyeva
The aim is to show the regulatory significance of promoter organs of morphogenesis in a stable state of the kidneys of vertebrates (fish, birds) and humans. Material and methods. 118 embryos at stages 12-23 of Carnegie (SC) and 28 human fetuses of 9-12 weeks of fetogenesis, 268 embryos of meat chickens (cross "Hibro PG +") from the stage of 48 hours to 20 days of broiler egg incubation, 50 fry of peled Coregonus peled were studied (Gmelin, 1788) at the stage of yolk sac atrophy (stage 36-37 according to the classification of Detlaf, 1975). To study the method of light microscopy, the material fixed in a 10% neutral form, embedded in paraffin. Sections stained with Mayer's hematoxylin and eosin, McManus CHIC method. Ki-67, CD31, CD34, positive cells are detected by immunohistochemistry. Primary kidney examination by low-vacuum electron microscopy. Embryos and human fetuses are observed in the Tyumen health care facility during medical abortion on demand in anamnestic healthy women with their warning about emergency work. Observation chicken embryos at the poultry farm “Tyumensky Broiler”, incubation workshop (Kaskara village, Tyumen region). Morphometric analysis and statistical processing of the actual material were carried out. Results. It was shown that the organotypic differentiation of the intermediate mesenchyme and the formation of nephrons of the provisional and definitive urinary organs control the state of the mesonephric duct and metanephritic diverticulum. The mesonephric duct initiates the organotypic antibiotic of the perifocal intermediate mesonephric mesenchyme and detects the proximodistal vector of saltatory mesonephronogenesis. The metanephritic diverticulum and its daughter branches initiate the organotypic differentiation of the intermediate mesenchyme and control the fan mechanism of metanephronogenesis. Activity of nephronogenesis Determination of the dynamics of convergence of immunocompetent cells. The absence of ventrodorsal generation of mesonephrons in humans is associated with a significant vital cycle of a normal kidney, atrophy of the mesonephric duct, and a decrease in the activity of immunocompetent cells. An increase in the expression of CD31, CD34, Bcl-2, Ki-67 positive cells is realized in combination with the formation of nephron rudiments as the promoter organs move in the developing organ of urinary formation. Conclusion. Prenatal organogenesis monitors and constantly monitors the formation of promoter organs of morphogenesis - the mesonephric duct and the metanephric disserticulum. Organotypic infection of the intermediate mesenchyme during the development of fish, birds and humans correlates with the chronovector of the convergence of immunocompetent cells.
{"title":"Morphogenetic Promoters in the Development of Provisional and Definitive Urinary Organs in Humans and Vertebrates","authors":"V. А. Аkhmatov, Yu. S. Spirina, D. S. Ledneva, I. А. Aptekar', А. А. Markov, А. N. Steblyuk, V. A. Shidin, G. S. Solovyev, A. R. Nurgalieva, О. G. Solovyeva","doi":"10.18499/2225-7357-2024-13-1-9-18","DOIUrl":"https://doi.org/10.18499/2225-7357-2024-13-1-9-18","url":null,"abstract":"The aim is to show the regulatory significance of promoter organs of morphogenesis in a stable state of the kidneys of vertebrates (fish, birds) and humans. Material and methods. 118 embryos at stages 12-23 of Carnegie (SC) and 28 human fetuses of 9-12 weeks of fetogenesis, 268 embryos of meat chickens (cross \"Hibro PG +\") from the stage of 48 hours to 20 days of broiler egg incubation, 50 fry of peled Coregonus peled were studied (Gmelin, 1788) at the stage of yolk sac atrophy (stage 36-37 according to the classification of Detlaf, 1975). To study the method of light microscopy, the material fixed in a 10% neutral form, embedded in paraffin. Sections stained with Mayer's hematoxylin and eosin, McManus CHIC method. Ki-67, CD31, CD34, positive cells are detected by immunohistochemistry. Primary kidney examination by low-vacuum electron microscopy. Embryos and human fetuses are observed in the Tyumen health care facility during medical abortion on demand in anamnestic healthy women with their warning about emergency work. Observation chicken embryos at the poultry farm “Tyumensky Broiler”, incubation workshop (Kaskara village, Tyumen region). Morphometric analysis and statistical processing of the actual material were carried out. Results. It was shown that the organotypic differentiation of the intermediate mesenchyme and the formation of nephrons of the provisional and definitive urinary organs control the state of the mesonephric duct and metanephritic diverticulum. The mesonephric duct initiates the organotypic antibiotic of the perifocal intermediate mesonephric mesenchyme and detects the proximodistal vector of saltatory mesonephronogenesis. The metanephritic diverticulum and its daughter branches initiate the organotypic differentiation of the intermediate mesenchyme and control the fan mechanism of metanephronogenesis. Activity of nephronogenesis Determination of the dynamics of convergence of immunocompetent cells. The absence of ventrodorsal generation of mesonephrons in humans is associated with a significant vital cycle of a normal kidney, atrophy of the mesonephric duct, and a decrease in the activity of immunocompetent cells. An increase in the expression of CD31, CD34, Bcl-2, Ki-67 positive cells is realized in combination with the formation of nephron rudiments as the promoter organs move in the developing organ of urinary formation. Conclusion. Prenatal organogenesis monitors and constantly monitors the formation of promoter organs of morphogenesis - the mesonephric duct and the metanephric disserticulum. Organotypic infection of the intermediate mesenchyme during the development of fish, birds and humans correlates with the chronovector of the convergence of immunocompetent cells.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"32 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140735563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-06DOI: 10.18499/2225-7357-2024-13-1-28-35
I. V. Gaivoronskii, А. А. Semenov, V. Krishtop, D. V. Ovchinnikov
The study of anthropometric indicators and body composition of various categories of the population makes it possible to monitor physical development, conduct health assessments and timely development of preventive measures. The purpose of the study is to evaluate the dynamics of indicators of the component composition of the body of military university cadets during the training period from 1st to 6th year. Material and methods. 387 male cadets (the same persons) were examined during the period of their studies in 1-6 courses. The component composition of the body was studied using bioimpedance measurements. Results. It has been shown that during the first two courses of study (persons 17-18 years old) there is an increase in muscle mass of the trunk by 13.8%, muscle mass of the upper extremities - by 11.4% and lower extremities - by 6.1%, which is explained by the presence intense physical activity. Moreover, during the first year of study, a slight increase in the fat component of the body is determined (5.4%). In the third and fourth years of study, there is a stabilization and slowdown in the growth of the formed muscle and fat masses of the trunk and limbs, characteristic of the transition period from adolescence to adulthood. These trends continued to increase throughout the 5th year of study, but the level of basal metabolism did not change significantly throughout the study. In the 6th year, in relation to the time of admission to university, a general increase in the muscle component of the torso was established by 17.2%, muscle mass of the upper extremities - by 15.8%, and lower extremities - by 9.6%. Over 6 years of study, the fat content in the torso increased by 8.2%, the fat mass of the upper and lower extremities - by 7.5% and 8.0%, respectively, and the level of visceral fat increased by 9.3%. The vital volume of the lungs increased by 12.2% compared to the beginning of the 1st course. Conclusion. The above transformations are obviously due to the continued growth of a healthy organism. Reduced adaptive changes during the training period can be corrected by increasing physical activity, diet and caloric intake.
{"title":"Dynamics of Body Component Composition Indicators in Males During Studying at the Military Medical University","authors":"I. V. Gaivoronskii, А. А. Semenov, V. Krishtop, D. V. Ovchinnikov","doi":"10.18499/2225-7357-2024-13-1-28-35","DOIUrl":"https://doi.org/10.18499/2225-7357-2024-13-1-28-35","url":null,"abstract":"The study of anthropometric indicators and body composition of various categories of the population makes it possible to monitor physical development, conduct health assessments and timely development of preventive measures. The purpose of the study is to evaluate the dynamics of indicators of the component composition of the body of military university cadets during the training period from 1st to 6th year. Material and methods. 387 male cadets (the same persons) were examined during the period of their studies in 1-6 courses. The component composition of the body was studied using bioimpedance measurements. Results. It has been shown that during the first two courses of study (persons 17-18 years old) there is an increase in muscle mass of the trunk by 13.8%, muscle mass of the upper extremities - by 11.4% and lower extremities - by 6.1%, which is explained by the presence intense physical activity. Moreover, during the first year of study, a slight increase in the fat component of the body is determined (5.4%). In the third and fourth years of study, there is a stabilization and slowdown in the growth of the formed muscle and fat masses of the trunk and limbs, characteristic of the transition period from adolescence to adulthood. These trends continued to increase throughout the 5th year of study, but the level of basal metabolism did not change significantly throughout the study. In the 6th year, in relation to the time of admission to university, a general increase in the muscle component of the torso was established by 17.2%, muscle mass of the upper extremities - by 15.8%, and lower extremities - by 9.6%. Over 6 years of study, the fat content in the torso increased by 8.2%, the fat mass of the upper and lower extremities - by 7.5% and 8.0%, respectively, and the level of visceral fat increased by 9.3%. The vital volume of the lungs increased by 12.2% compared to the beginning of the 1st course. Conclusion. The above transformations are obviously due to the continued growth of a healthy organism. Reduced adaptive changes during the training period can be corrected by increasing physical activity, diet and caloric intake.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"27 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140734315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-06DOI: 10.18499/2225-7357-2024-13-1-51-56
А. А. Lytkina, D. K. Garmaeva
The paper highlights the study results related to the structural features of the thyroid gland (TG) based on ultrasound examination findings and their relationship with somatotypological parameters in young men living in the Republic of Sakha (Yakutia). Since diagnosis of thyroid pathology is associated with ultrasound examination, advanced information about the regional features of the anatomical standad parameters of the thyroid gland remains an acute research and practical issue. The aim of the study was to identify the morphometric parameter features of the thyroid gland during ultrasound scanning and the nature of their relationship with the somatotype in young men living in the Republic of Sakha (Yakutia), indigenous and non-indigenous ethnic groups. Material and methods. The study involved total 195 males, aged 17-21, who were divided into groups depending on indigenous and non-indigenous ethnicity. All subjects underwent anthropometric measurements of 10 parameters, followed by determination of the somatotype according to Heath-Carter. To determine the linear dimensions and volume of the thyroid gland, the ultrasound diagnostic option was used. Results. As stated, representatives of the mesomorphic somatotype predominate among young men of the indigenous ethnic group; representatives of the ectomorphic somatotype predominate among young men of the non-indigenous ethnic group. A comparative analysis of ultrasound morphometry revealed significantly larger values of the total volume of the thyroid gland, due to an increased thickness and volume of the right lobe, as well as the thickness, width and volume of the left lobe in young men of the non-indigenous ethnic group, compared with young men of the indigenous ethnic group. When comparing the thyroid volume with the identified distribution of somatotypes according to Heath-Carter, statistically significant differences were registered in representatives of the mesomorphic somatotype: in young men of the non-indigenous ethnic group, the volume was 2.1 cm3 greater than in young men of the indigenous ethnic group. Conclusion. Thus, the obtained findings related to linear parameters and thyroid volume evidence their relationship with ethnicity and somatotype.
{"title":"Dependence of the Thyroid Gland Ultrasound Findings on Somatotypological Parameters in Young Men Living in the Republic of Sakha (Yakutia)","authors":"А. А. Lytkina, D. K. Garmaeva","doi":"10.18499/2225-7357-2024-13-1-51-56","DOIUrl":"https://doi.org/10.18499/2225-7357-2024-13-1-51-56","url":null,"abstract":"The paper highlights the study results related to the structural features of the thyroid gland (TG) based on ultrasound examination findings and their relationship with somatotypological parameters in young men living in the Republic of Sakha (Yakutia). Since diagnosis of thyroid pathology is associated with ultrasound examination, advanced information about the regional features of the anatomical standad parameters of the thyroid gland remains an acute research and practical issue. The aim of the study was to identify the morphometric parameter features of the thyroid gland during ultrasound scanning and the nature of their relationship with the somatotype in young men living in the Republic of Sakha (Yakutia), indigenous and non-indigenous ethnic groups. Material and methods. The study involved total 195 males, aged 17-21, who were divided into groups depending on indigenous and non-indigenous ethnicity. All subjects underwent anthropometric measurements of 10 parameters, followed by determination of the somatotype according to Heath-Carter. To determine the linear dimensions and volume of the thyroid gland, the ultrasound diagnostic option was used. Results. As stated, representatives of the mesomorphic somatotype predominate among young men of the indigenous ethnic group; representatives of the ectomorphic somatotype predominate among young men of the non-indigenous ethnic group. A comparative analysis of ultrasound morphometry revealed significantly larger values of the total volume of the thyroid gland, due to an increased thickness and volume of the right lobe, as well as the thickness, width and volume of the left lobe in young men of the non-indigenous ethnic group, compared with young men of the indigenous ethnic group. When comparing the thyroid volume with the identified distribution of somatotypes according to Heath-Carter, statistically significant differences were registered in representatives of the mesomorphic somatotype: in young men of the non-indigenous ethnic group, the volume was 2.1 cm3 greater than in young men of the indigenous ethnic group. Conclusion. Thus, the obtained findings related to linear parameters and thyroid volume evidence their relationship with ethnicity and somatotype.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"5 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140735537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.18499/2225-7357-2023-12-4-98-102
E. V. Belov, N. N. Patlataya, V. A. Bolotskikh, K. I. Obernikhin, D. A. Sokolov, A. Kvaratskheliya
Anthropometric data provide a qualitative and quantitative assessment of the health status of the population. Typically, they are used in clinical practice to assess general health, nutritional adequacy, growth and development of the body. The aim is to conduct a comparative analysis of anthropometric indicators and proportionality of the physique of boys and girls living in Moscow and the Moscow region. Material and methods. The study involved 198 third-year students of the Faculty of Medicine of the State University of Education, over the age of 18, living in Moscow and the Moscow region. The surveyed population did not have either acute or chronic diseases in the stage of decompensation, congenital malformations, ultra-low or excess body weight. An anthropometric study was carried out with measurements of the longitudinal and transverse dimensions of the torso and limbs, calculation of the body mass index, determination of the somatotype and subsequent statistical processing of the data obtained. Conclusion. Anthropometric indicators of girls and boys living in the Moscow region have a number of features. Persons with a mesomorphic somatotype predominate, which indicates the harmonious physical development of boys and girls. Most girls have a transversely narrowed shape of the pelvis, which indicates a possible risk of obstetric complications in the event of pregnancy and childbirth. Otherwise, both boys and girls develop proportionally in accordance with the parameters of the biological and physiological norm.
{"title":"Anthropometric Indicators of the Proportionality of the Physique of Girls and Boys Living in the Moscow Region","authors":"E. V. Belov, N. N. Patlataya, V. A. Bolotskikh, K. I. Obernikhin, D. A. Sokolov, A. Kvaratskheliya","doi":"10.18499/2225-7357-2023-12-4-98-102","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-4-98-102","url":null,"abstract":"Anthropometric data provide a qualitative and quantitative assessment of the health status of the population. Typically, they are used in clinical practice to assess general health, nutritional adequacy, growth and development of the body. The aim is to conduct a comparative analysis of anthropometric indicators and proportionality of the physique of boys and girls living in Moscow and the Moscow region. Material and methods. The study involved 198 third-year students of the Faculty of Medicine of the State University of Education, over the age of 18, living in Moscow and the Moscow region. The surveyed population did not have either acute or chronic diseases in the stage of decompensation, congenital malformations, ultra-low or excess body weight. An anthropometric study was carried out with measurements of the longitudinal and transverse dimensions of the torso and limbs, calculation of the body mass index, determination of the somatotype and subsequent statistical processing of the data obtained. Conclusion. Anthropometric indicators of girls and boys living in the Moscow region have a number of features. Persons with a mesomorphic somatotype predominate, which indicates the harmonious physical development of boys and girls. Most girls have a transversely narrowed shape of the pelvis, which indicates a possible risk of obstetric complications in the event of pregnancy and childbirth. Otherwise, both boys and girls develop proportionally in accordance with the parameters of the biological and physiological norm.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"2 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139525648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.18499/2225-7357-2023-12-4-68-75
T. N. Sotnikova, T. V. Polushkina, D. Kalinin, N. Danilova
The aim is to study the relationship of PD-L1 status of SP142 and SP263 clones in gastric cancer with its clinical and morphological parameters. Material and methods. Surgical material obtained from 131 patients with a confirmed diagnosis of stomach cancer. Antibodies to PD-L1 SP263 were stained 127 cases, antibodies to PD-L1 SP142 – 126 cases. Reactions with PD-L1 were performed using the Ventana BenchMark Ultra device (Roche Ventana, USA) according to the protocols recommended by the manufacturer of primary antibodies. The results of immunohistochemical staining were compared with the clinical and morphological characteristics of gastric cancer using statistical analysis. Results. The positive PD-L1 status detected by clones SP263 and SP142 is significantly associated with type 2 macroscopic form according to the R. Bormann classification (p=0,003/p=0,003), tubular morphological type according to the WHO 5th edition, 2019 classification (p=0,001/p=0,018), intermediate morphological type according to the classification of P. Lauren (p=0,027/p=0,005) and the absence of signet ring cells (p=0,001/p=0,010). Staining with both clones used was not associated with the gender and age of patients, the size and localization of the tumor, the degree of its differentiation, the presence or absence of emboli in the lumen of blood and lymph vessels, the presence of affected lymph nodes and distant metastases, as well as the clinical stage (p>0,05). The largest proportion of PD-L1 positive cases in the study of SP263/SP142 clones was found in tubular type tumors (75,6%/84,6%) according to the WHO 5th edition, 2019 classification, intermediate type (60,0%/68,0%) according to P. Lauren, type 2 (65,4%/68,0%) according to R. Bormann and in the absence of signet ring cells (70,7%/76,9%). Conclusion. Statistically significant clinical and morphological parameters of gastric cancer, where a reliable relationship between them and the expression of PD-L1 clones SP263 and SP142 was revealed, are: type 2 macroscopic form according to R. Bormann (p=0,003/p=0,003), tubular morphological type according to the classification of WHO 5th edition, 2019) (p=0,001/p=0,018), intermediate type according to P. Lauren (p=0,027/p=0,005) and the absence of signet ring cells (p=0,001/p=0,010).
{"title":"Association Between PD-L1 status Detected by SP142 and SP263 Antibody Clones and Clinical and Morphological Features of Gastric Cancer Factors","authors":"T. N. Sotnikova, T. V. Polushkina, D. Kalinin, N. Danilova","doi":"10.18499/2225-7357-2023-12-4-68-75","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-4-68-75","url":null,"abstract":"The aim is to study the relationship of PD-L1 status of SP142 and SP263 clones in gastric cancer with its clinical and morphological parameters. Material and methods. Surgical material obtained from 131 patients with a confirmed diagnosis of stomach cancer. Antibodies to PD-L1 SP263 were stained 127 cases, antibodies to PD-L1 SP142 – 126 cases. Reactions with PD-L1 were performed using the Ventana BenchMark Ultra device (Roche Ventana, USA) according to the protocols recommended by the manufacturer of primary antibodies. The results of immunohistochemical staining were compared with the clinical and morphological characteristics of gastric cancer using statistical analysis. Results. The positive PD-L1 status detected by clones SP263 and SP142 is significantly associated with type 2 macroscopic form according to the R. Bormann classification (p=0,003/p=0,003), tubular morphological type according to the WHO 5th edition, 2019 classification (p=0,001/p=0,018), intermediate morphological type according to the classification of P. Lauren (p=0,027/p=0,005) and the absence of signet ring cells (p=0,001/p=0,010). Staining with both clones used was not associated with the gender and age of patients, the size and localization of the tumor, the degree of its differentiation, the presence or absence of emboli in the lumen of blood and lymph vessels, the presence of affected lymph nodes and distant metastases, as well as the clinical stage (p>0,05). The largest proportion of PD-L1 positive cases in the study of SP263/SP142 clones was found in tubular type tumors (75,6%/84,6%) according to the WHO 5th edition, 2019 classification, intermediate type (60,0%/68,0%) according to P. Lauren, type 2 (65,4%/68,0%) according to R. Bormann and in the absence of signet ring cells (70,7%/76,9%). Conclusion. Statistically significant clinical and morphological parameters of gastric cancer, where a reliable relationship between them and the expression of PD-L1 clones SP263 and SP142 was revealed, are: type 2 macroscopic form according to R. Bormann (p=0,003/p=0,003), tubular morphological type according to the classification of WHO 5th edition, 2019) (p=0,001/p=0,018), intermediate type according to P. Lauren (p=0,027/p=0,005) and the absence of signet ring cells (p=0,001/p=0,010).","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"5 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139526055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.18499/2225-7357-2023-12-4-108-114
M. Mnikhovich, P. A. Askhanova, T. V. Bezuglova, I. A. Shiripenko, O. A. Sidorova, M. V. Lozina
“Thanatology is the study of the signs, dynamics and statics of death,” this is a definition given by Georgy V. Shore to the science, to which he devoted his career. The professor was the father of the autopsy in Russia, made a huge contribution to the development of medical education, and strived for constant improvement of diagnostic and treatment options. His unique approach to the study of medicine was based on the intersection of clinic and morphology and attracted many followers. Georgy V. Shore devoted a significant part of his work to developing techniques for preserving anatomical museum specimens. As a dissector, Georgy V. Shore contributed greatly to the invention of new autopsy techniques to facilitate and improve the work of pathologists; and today we use the approaches he developed. The ideas presented in his “Teaching of Death” are the quintessence of the scientific thought of Georgy V. Shor and are still discussed by the medical community. Where people saw only death, G.V. Shor was discovering something new for life. This year is the 150th anniversary of Georgy V. Shor’s birth; it is worth remembering the significant events associated with the name of this brilliant scientist
"死亡学是对死亡的征兆、动态和静态的研究"，这是乔治-V-肖尔对这门他为之献身的科学所下的定义。这位教授是俄罗斯尸检之父，为医学教育的发展做出了巨大贡献，并致力于不断改进诊断和治疗方案。他独特的医学研究方法以临床和形态学的交叉为基础，吸引了众多追随者。Georgy V. Shore 的很大一部分工作是开发保存解剖博物馆标本的技术。作为一名解剖学家，Georgy V. Shore 为发明新的解剖技术以促进和改进病理学家的工作做出了巨大贡献；今天，我们仍在使用他开发的方法。他在《死亡教学》中提出的观点是乔治-V-肖尔科学思想的精髓，至今仍为医学界所讨论。在人们只看到死亡的地方，G.V. 肖尔却发现了生命的新东西。今年是 Georgy V. Shor 诞辰 150 周年，值得纪念的是与这位杰出科学家名字相关的重要事件
{"title":"Life and Scientific Achievements of an Outstanding Pathologist. To the 150th Anniversary of Georgy V. Shor’s Birth","authors":"M. Mnikhovich, P. A. Askhanova, T. V. Bezuglova, I. A. Shiripenko, O. A. Sidorova, M. V. Lozina","doi":"10.18499/2225-7357-2023-12-4-108-114","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-4-108-114","url":null,"abstract":"“Thanatology is the study of the signs, dynamics and statics of death,” this is a definition given by Georgy V. Shore to the science, to which he devoted his career. The professor was the father of the autopsy in Russia, made a huge contribution to the development of medical education, and strived for constant improvement of diagnostic and treatment options. His unique approach to the study of medicine was based on the intersection of clinic and morphology and attracted many followers. Georgy V. Shore devoted a significant part of his work to developing techniques for preserving anatomical museum specimens. As a dissector, Georgy V. Shore contributed greatly to the invention of new autopsy techniques to facilitate and improve the work of pathologists; and today we use the approaches he developed. The ideas presented in his “Teaching of Death” are the quintessence of the scientific thought of Georgy V. Shor and are still discussed by the medical community. Where people saw only death, G.V. Shor was discovering something new for life. This year is the 150th anniversary of Georgy V. Shor’s birth; it is worth remembering the significant events associated with the name of this brilliant scientist","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139524979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.18499/2225-7357-2023-12-4-103-107
E. D. Lutsai, S. Naidenova, I. V. Astaf’ev, T. N. Dal’
Among the anomalies of the optic disc development, which change its anatomical characteristics, are the druses of the optic disc. Diagnostically, the druses of the optic disc are manifested by protrusion into the vitreous body, and changing in the area of the optic disc and its excavation. The aim of the study is to obtain new data on the optic disc anatomy in elderly men. Material and methods. The study was conducted in a group of men from 61 to 75 years old. The study included 53 eyes of patients treated with a disease of the visual organ unrelated to the pathology of the optic disc and macula. The study was carried out on the Optovue RTVue XR Avanti Sistem device using standard 3D Disc, GCC, ONH programs. Using optical coherence tomography the following morphometric parameters of the optic disc were evaluated: the volume of excavation, the thickness of the retinal nerve fiber layer, the area of the optic disc, the depth, the width and the area of the excavation of the optic disc in the horizontal plane. Conclusion. In the group of men from 61 to 75 years old, with increasing age, there is a decrease in the thickness of the retinal nerve fiber layer, the width of the excavation of the optic disc and the area of the optic disc. The shapes of optic disc excavation in elderly men are different, the triangular shape prevails – 47,8%, the remaining shapes occur with the same frequency.
{"title":"Features of the Optic Disc Anatomy in Elderly Men","authors":"E. D. Lutsai, S. Naidenova, I. V. Astaf’ev, T. N. Dal’","doi":"10.18499/2225-7357-2023-12-4-103-107","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-4-103-107","url":null,"abstract":"Among the anomalies of the optic disc development, which change its anatomical characteristics, are the druses of the optic disc. Diagnostically, the druses of the optic disc are manifested by protrusion into the vitreous body, and changing in the area of the optic disc and its excavation. The aim of the study is to obtain new data on the optic disc anatomy in elderly men. Material and methods. The study was conducted in a group of men from 61 to 75 years old. The study included 53 eyes of patients treated with a disease of the visual organ unrelated to the pathology of the optic disc and macula. The study was carried out on the Optovue RTVue XR Avanti Sistem device using standard 3D Disc, GCC, ONH programs. Using optical coherence tomography the following morphometric parameters of the optic disc were evaluated: the volume of excavation, the thickness of the retinal nerve fiber layer, the area of the optic disc, the depth, the width and the area of the excavation of the optic disc in the horizontal plane. Conclusion. In the group of men from 61 to 75 years old, with increasing age, there is a decrease in the thickness of the retinal nerve fiber layer, the width of the excavation of the optic disc and the area of the optic disc. The shapes of optic disc excavation in elderly men are different, the triangular shape prevails – 47,8%, the remaining shapes occur with the same frequency.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"10 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139525125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.18499/2225-7357-2023-12-4-76-88
N. Shevlyuk, E. V. Blinova, R. E. Egemberdieva, Z. Komekbai, M. F. Ryskulov, D. A. Bokov, A. S. Maksimova
The aim of the study is a comparative analysis of the morphofunctional interactions of germ and somatic cells in the organs of the male reproductive system of vertebrates during ontogenesis, under conditions of changes in reproductive activity and under the influence of destabilizing factors. Material and methods. Using light and electron microscopy, immunohistochemistry and morphometry, the testes of representatives of various classes of vertebrates (amphibians, reptiles, birds and mammals) were studied. The collection of material was carried out in environmentally safe ecosystems and in ecosystems with high anthropogenic load in the Orenburg region of Russia and in the Aktobe region of the Republic of Kazakhstan. Results. Based on the material of our own studies of the gonads, as well as on the basis of information from modern domestic and foreign literature, the patterns of relationships between germ cells and somatic cells of their microenvironment in the testes of vertebrates are shown. The features of the structural organization of the germinal and endocrine components of the male gonads are considered. The features of the structural organization of intertitial endocrinocytes (Leydig cells) are shown, and their high resistance to the action of various destabilizing factors compared to spermatogenic epithelium is established. Pubertal generation of endocrinocytes in vertebrate testes during reproductive age is characterized by stability in cell numbers and belongs to cell populations of a stable type. The role and significance of the structures of the blood-testis barrier are shown and the morphological equivalents of changes in the permeability of this barrier are established; it is revealed that in the studied animals, under the influence of negative anthropogenic factors, the integrity of the blood-testis barrier in the testes is violated. The action of destabilizing factors leads to a decrease in the number of developing germ cells in the lumen of the epididymal tubules (up to their complete disappearance in individual individuals), which is probably due to the deterioration of the trophic influences on them from the structures of the epididymis. Conclusion. The principle of organization of the main compartments of the testes, the convoluted seminiferous tubules and the interstitium of the organ, formed during evolution, probably turned out to be evolutionarily advantageous, since it is represented in the testes of all amniotes - reptiles, birds and mammals. In the process of evolutionary development, several types of structural organization of convoluted seminiferous tubules and interstitium were formed in the testes of vertebrates. The need to protect developing germ cells from the effects of immunocytes, which led to the formation of a blood-testis barrier in the testes.
{"title":"The Relationship of Gametes and Somatic Cells of Male Gonads of Vertebrates: Evolutionary, Age and Seasonal Aspects, Adaptive and Reactive Transformations Under the Action of Destabilizing Factors","authors":"N. Shevlyuk, E. V. Blinova, R. E. Egemberdieva, Z. Komekbai, M. F. Ryskulov, D. A. Bokov, A. S. Maksimova","doi":"10.18499/2225-7357-2023-12-4-76-88","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-4-76-88","url":null,"abstract":"The aim of the study is a comparative analysis of the morphofunctional interactions of germ and somatic cells in the organs of the male reproductive system of vertebrates during ontogenesis, under conditions of changes in reproductive activity and under the influence of destabilizing factors. Material and methods. Using light and electron microscopy, immunohistochemistry and morphometry, the testes of representatives of various classes of vertebrates (amphibians, reptiles, birds and mammals) were studied. The collection of material was carried out in environmentally safe ecosystems and in ecosystems with high anthropogenic load in the Orenburg region of Russia and in the Aktobe region of the Republic of Kazakhstan. Results. Based on the material of our own studies of the gonads, as well as on the basis of information from modern domestic and foreign literature, the patterns of relationships between germ cells and somatic cells of their microenvironment in the testes of vertebrates are shown. The features of the structural organization of the germinal and endocrine components of the male gonads are considered. The features of the structural organization of intertitial endocrinocytes (Leydig cells) are shown, and their high resistance to the action of various destabilizing factors compared to spermatogenic epithelium is established. Pubertal generation of endocrinocytes in vertebrate testes during reproductive age is characterized by stability in cell numbers and belongs to cell populations of a stable type. The role and significance of the structures of the blood-testis barrier are shown and the morphological equivalents of changes in the permeability of this barrier are established; it is revealed that in the studied animals, under the influence of negative anthropogenic factors, the integrity of the blood-testis barrier in the testes is violated. The action of destabilizing factors leads to a decrease in the number of developing germ cells in the lumen of the epididymal tubules (up to their complete disappearance in individual individuals), which is probably due to the deterioration of the trophic influences on them from the structures of the epididymis. Conclusion. The principle of organization of the main compartments of the testes, the convoluted seminiferous tubules and the interstitium of the organ, formed during evolution, probably turned out to be evolutionarily advantageous, since it is represented in the testes of all amniotes - reptiles, birds and mammals. In the process of evolutionary development, several types of structural organization of convoluted seminiferous tubules and interstitium were formed in the testes of vertebrates. The need to protect developing germ cells from the effects of immunocytes, which led to the formation of a blood-testis barrier in the testes.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"8 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139524953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-19DOI: 10.18499/2225-7357-2023-12-4-89-97
I. Polovnikov, G. Yukina, E. G. Sukhorukova
Lymphangioleiomyomatosis (LAM) is a polysystemic disease based on the formation of granulomas prone to degenerate into cysts in the lungs, abdominal and pelvic organs. Lung involvement in LAM is accompanied by respiratory dysfunction and can be fatal. WHO classifies this disease into a group of mesenchymal tumors and considers it as a low-grade destructive metastasizing neoplasm. LAM is a rare disease and earlier it was only detected in women, but nowadays cases of pulmonary LAM in men (include teenagers) have been described. The etiology of LAM and the inducing factors are still unknown. Objective – to collect and systematize data on pathomorphological characteristic of changes in lung tissue in LAM to evaluate the completeness and sufficiency of the available data. Material and methods. There were analyzed 912 articles and monographs (including duplicates) from PubMed, eLibrary, and Cyberleninka databases over a fifty-year period from 1973 to August of 2023, with an emphasis on more relevant publications and articles in highly rated scientific journals that include information on the review topic. Eventually 55 original sources were selected. Results. The article presents summary data on macroscopic and microscopic picture of pulmonary LAM, data on morphological and antigenic characterization of cells forming the basis of granulomas and their surroundings, the results of experiments demonstrating dynamics of pathological changes in LAM. Conclusion. Despite the relatively large amount of data about morphological characteristics that is enough for diagnosis, he main issue is still open: what is the origin of LAM cells? A systematic pathomorphology analyze can help us to find an answer for this question.
{"title":"Pathomorphological Characteristic of Pulmonary Lymphangioleiomyomatosis","authors":"I. Polovnikov, G. Yukina, E. G. Sukhorukova","doi":"10.18499/2225-7357-2023-12-4-89-97","DOIUrl":"https://doi.org/10.18499/2225-7357-2023-12-4-89-97","url":null,"abstract":"Lymphangioleiomyomatosis (LAM) is a polysystemic disease based on the formation of granulomas prone to degenerate into cysts in the lungs, abdominal and pelvic organs. Lung involvement in LAM is accompanied by respiratory dysfunction and can be fatal. WHO classifies this disease into a group of mesenchymal tumors and considers it as a low-grade destructive metastasizing neoplasm. LAM is a rare disease and earlier it was only detected in women, but nowadays cases of pulmonary LAM in men (include teenagers) have been described. The etiology of LAM and the inducing factors are still unknown. Objective – to collect and systematize data on pathomorphological characteristic of changes in lung tissue in LAM to evaluate the completeness and sufficiency of the available data. Material and methods. There were analyzed 912 articles and monographs (including duplicates) from PubMed, eLibrary, and Cyberleninka databases over a fifty-year period from 1973 to August of 2023, with an emphasis on more relevant publications and articles in highly rated scientific journals that include information on the review topic. Eventually 55 original sources were selected. Results. The article presents summary data on macroscopic and microscopic picture of pulmonary LAM, data on morphological and antigenic characterization of cells forming the basis of granulomas and their surroundings, the results of experiments demonstrating dynamics of pathological changes in LAM. Conclusion. Despite the relatively large amount of data about morphological characteristics that is enough for diagnosis, he main issue is still open: what is the origin of LAM cells? A systematic pathomorphology analyze can help us to find an answer for this question.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139525882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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