Pub Date : 2023-01-15DOI: 10.18499/2225-7357-2022-11-4-79-82
V. Shestakova, A. G. Ivanov
{"title":"Morphology day in Tver land","authors":"V. Shestakova, A. G. Ivanov","doi":"10.18499/2225-7357-2022-11-4-79-82","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-79-82","url":null,"abstract":"","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75188424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-15DOI: 10.18499/2225-7357-2022-11-4-63-66
T. S. Guseinov, S. Guseinova
{"title":"90th anniversary of the department of human anatomy of Dagestan State Medical University","authors":"T. S. Guseinov, S. Guseinova","doi":"10.18499/2225-7357-2022-11-4-63-66","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-63-66","url":null,"abstract":"","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75081344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-15DOI: 10.18499/2225-7357-2022-11-4-76-78
N. Shevlyuk
{"title":"Review of a scientific monograph of A. V. El'chaninov, T. Kh. Fatkhudinov “Mammalian liver regeneration. Intercellular interactions [Regeneratsiya pecheni mlekopitayushchikh. Mezhkletochnye vzaimodeistviya]”. M.: Nauka, 2020","authors":"N. Shevlyuk","doi":"10.18499/2225-7357-2022-11-4-76-78","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-76-78","url":null,"abstract":"","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"70 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72587406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-15DOI: 10.18499/2225-7357-2022-11-4-67-75
A. A. Shevchenko, N. Alexeeva, D. Nikityuk, S. Klochkova, D. A. Sokolov, A. Kvaratskheliya, A. V. Karpova
{"title":"Hic locus ubi mortui docent vivos. To the 135th anniversary of the birth of I. V. Davydovsky (1887–1968)","authors":"A. A. Shevchenko, N. Alexeeva, D. Nikityuk, S. Klochkova, D. A. Sokolov, A. Kvaratskheliya, A. V. Karpova","doi":"10.18499/2225-7357-2022-11-4-67-75","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-67-75","url":null,"abstract":"","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90548021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-15DOI: 10.18499/2225-7357-2022-11-4-58-62
V. A. Raptanova, P. S. Bobkov, A. V. Droblenkov
{"title":"Structural features of ghrelin-producing endocrine cells and their reactive changes after experimental stress","authors":"V. A. Raptanova, P. S. Bobkov, A. V. Droblenkov","doi":"10.18499/2225-7357-2022-11-4-58-62","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-58-62","url":null,"abstract":"","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79798349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-13DOI: 10.18499/2225-7357-2022-11-4-20-26
O. V. Guseva, N. V. Bashmalukh, V. Polyakova, T. V. Nikolaeva
The aim of the study was to determine the temporal sequence of structural and functional reorganization of hair follicles in the skin of C57BL/6 mice after anagen induction.Material and methods. 30 male mice of the C57BL/6 inbred line were used for the study. Anagen induction was carried out by depilation of the hair shafts of the skin of the back. Mice were withdrawn from the experiment on the 1st, 5th, 9th, 15th, 19th, 28th days after anagen induction. Skin samples of the depilation area were fixed in buffered neutral formalin and examined using survey microscopy, morphometry, immunohistochemistry, and statistics.Results. The study of the morphological picture from the 1st to the 28th day after anagen induction showed that cyclic changes in the thickness of the dermis, subcutaneous adipose tissue and the structure of hair follicles occur in the skin. On the 1st day after anagen induction the synchronous entry of hair follicles into the anagen stage I was observed: hair follicles were completely in the dermis, had a rounded dermal papilla, above which, towards the epidermis, there was a proliferating follicular epithelium in the form of an expanding and elongating strand . On the 5th day, morphological signs corresponding to the stage of anagen III b were observed: hair follicle bulbs were located in the upper third of the subcutaneous tissue. The dermal papillae looked friable and enlarged, around their upper pole there was a zone of melanogenesis, a hair shaft and an internal root epithelial sheath being formed. The morphological changes on the 9th and 15th days corresponded to the anagen stage VI: the tips of the pigmented hair rods emerged from the funnels of the hair follicles to the surface of the epidermis, the hair follicles were located deep in the subcutaneous fatty tissue, reaching the subcutaneous muscle. The hair follicles had a narrow dermal papilla, a zone of melanogenesis, and the forming hair shafts had regular pigmentation. A pronounced thickening of the dermis and subcutaneous adipose tissue was noted. On the 19th day, the hair follicles were in the catagen VI phase: two-row bag-like structures were observed, forming capsules of the secondary hair germ). They surrounded the keratinized brush-like depigmented proximal ends of the hair shafts. Hair follicles with a rounded dermal papilla were located in the middle of the subcutaneous adipose tissue. The dermal papillae were separated from the capsule of the secondary hair germ by a cord of follicular keratinocytes. Proximal to the dermal papillae, elements of the connective tissue sac were visible. On the 28th day, corresponding to the telogen stage, the hair follicles were located completely in the dermis, had a rounded, dense dermal papilla, which was adjacent to the germinal capsule. On the 19th and 28th days after anagen induction, a decrease in the width of the dermis and subcutaneous fat was noted.Conclusions. Thus, during the cycle of the hair follicle, its stage-by-stage
{"title":"Morphofunctional reorganization of hair follicles in C57BL/6 mice at different stages of development","authors":"O. V. Guseva, N. V. Bashmalukh, V. Polyakova, T. V. Nikolaeva","doi":"10.18499/2225-7357-2022-11-4-20-26","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-20-26","url":null,"abstract":"The aim of the study was to determine the temporal sequence of structural and functional reorganization of hair follicles in the skin of C57BL/6 mice after anagen induction.Material and methods. 30 male mice of the C57BL/6 inbred line were used for the study. Anagen induction was carried out by depilation of the hair shafts of the skin of the back. Mice were withdrawn from the experiment on the 1st, 5th, 9th, 15th, 19th, 28th days after anagen induction. Skin samples of the depilation area were fixed in buffered neutral formalin and examined using survey microscopy, morphometry, immunohistochemistry, and statistics.Results. The study of the morphological picture from the 1st to the 28th day after anagen induction showed that cyclic changes in the thickness of the dermis, subcutaneous adipose tissue and the structure of hair follicles occur in the skin. On the 1st day after anagen induction the synchronous entry of hair follicles into the anagen stage I was observed: hair follicles were completely in the dermis, had a rounded dermal papilla, above which, towards the epidermis, there was a proliferating follicular epithelium in the form of an expanding and elongating strand . On the 5th day, morphological signs corresponding to the stage of anagen III b were observed: hair follicle bulbs were located in the upper third of the subcutaneous tissue. The dermal papillae looked friable and enlarged, around their upper pole there was a zone of melanogenesis, a hair shaft and an internal root epithelial sheath being formed. The morphological changes on the 9th and 15th days corresponded to the anagen stage VI: the tips of the pigmented hair rods emerged from the funnels of the hair follicles to the surface of the epidermis, the hair follicles were located deep in the subcutaneous fatty tissue, reaching the subcutaneous muscle. The hair follicles had a narrow dermal papilla, a zone of melanogenesis, and the forming hair shafts had regular pigmentation. A pronounced thickening of the dermis and subcutaneous adipose tissue was noted. On the 19th day, the hair follicles were in the catagen VI phase: two-row bag-like structures were observed, forming capsules of the secondary hair germ). They surrounded the keratinized brush-like depigmented proximal ends of the hair shafts. Hair follicles with a rounded dermal papilla were located in the middle of the subcutaneous adipose tissue. The dermal papillae were separated from the capsule of the secondary hair germ by a cord of follicular keratinocytes. Proximal to the dermal papillae, elements of the connective tissue sac were visible. On the 28th day, corresponding to the telogen stage, the hair follicles were located completely in the dermis, had a rounded, dense dermal papilla, which was adjacent to the germinal capsule. On the 19th and 28th days after anagen induction, a decrease in the width of the dermis and subcutaneous fat was noted.Conclusions. Thus, during the cycle of the hair follicle, its stage-by-stage","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73751718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-13DOI: 10.18499/2225-7357-2022-11-4-48-52
S. Vinokurova, D. Garmaeva, L. Arzhakova, V. Makarova, A. Lytkina, T. K. Garmaev
The aim was to evaluate the indicators of physical development and quality of life in students of M.K. Ammosov North-Eastern Federal University (NEFU).Material and methods. The study involved 755 students of NEFU. at the age of 17–20, Mongoloid and Europeoid races, among which there were 273 (36.16%) girls and 482 (63.84%) boys. The studies were conducted on the basis of voluntary informed written consent. The survey included the determination of anthropometric parameters, assessment of the quality of life and questionnaires. Identification of the main characteristics of students' health was carried out through a medical examination.Results. An analysis of morphological indicators revealed differences in the physical development of students of the Mongoloid and Europeoid races of the Republic of Sakha (Yakutia). In the analyzed groups, there were significant differences in body length depending on race. The highest indicators of body length are determined in persons of the Europeoid race, the smallest values - in students of the Mongoloid race. The study of quality of life indicators found a decrease in the psychological component of health among the respondents, due to the lack of readiness of the components of the professional and personal perspective and the inability to carry out psychological regulation of their behavior, which serves as an obstacle to their adaptation to independent living. At the same time, the indicators of the physical component of the health of the examined students remain, in most cases, within the normal range.Conclusion. Analysis of the results of the study of the physical development of NEFU students indicates that representatives of the Mongoloid race living in the territory of Yakutia are characterized by growth below average, low density, and a small body surface area compared to representatives of the Europeoid race. The level of physical development in most of the examined persons is assessed as average, and in 37.2% of students the functional state of the circulatory system is characterized by tension in the mechanisms of adaptation, which may be due to the adaptation of the body to new living conditions.
{"title":"Evaluation of indicators of physical development and quality of life of students of M. K. Ammosov North-Eastern Federal University","authors":"S. Vinokurova, D. Garmaeva, L. Arzhakova, V. Makarova, A. Lytkina, T. K. Garmaev","doi":"10.18499/2225-7357-2022-11-4-48-52","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-48-52","url":null,"abstract":"The aim was to evaluate the indicators of physical development and quality of life in students of M.K. Ammosov North-Eastern Federal University (NEFU).Material and methods. The study involved 755 students of NEFU. at the age of 17–20, Mongoloid and Europeoid races, among which there were 273 (36.16%) girls and 482 (63.84%) boys. The studies were conducted on the basis of voluntary informed written consent. The survey included the determination of anthropometric parameters, assessment of the quality of life and questionnaires. Identification of the main characteristics of students' health was carried out through a medical examination.Results. An analysis of morphological indicators revealed differences in the physical development of students of the Mongoloid and Europeoid races of the Republic of Sakha (Yakutia). In the analyzed groups, there were significant differences in body length depending on race. The highest indicators of body length are determined in persons of the Europeoid race, the smallest values - in students of the Mongoloid race. The study of quality of life indicators found a decrease in the psychological component of health among the respondents, due to the lack of readiness of the components of the professional and personal perspective and the inability to carry out psychological regulation of their behavior, which serves as an obstacle to their adaptation to independent living. At the same time, the indicators of the physical component of the health of the examined students remain, in most cases, within the normal range.Conclusion. Analysis of the results of the study of the physical development of NEFU students indicates that representatives of the Mongoloid race living in the territory of Yakutia are characterized by growth below average, low density, and a small body surface area compared to representatives of the Europeoid race. The level of physical development in most of the examined persons is assessed as average, and in 37.2% of students the functional state of the circulatory system is characterized by tension in the mechanisms of adaptation, which may be due to the adaptation of the body to new living conditions.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74880761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-13DOI: 10.18499/2225-7357-2022-11-4-33-40
K. N. Pustovaya, G. A. Pyavchenko, N. Alexeeva, I. Kozlov, Z. V. Shamitko, V. A. Kovrigin, V. Nozdrin, S. Kuznetsov
The aim of this study was to study the morphofunctional changes in human skin with acneiform dermatoses.Material and methods. We studied the material obtained from 6 volunteers of both sexes aged 26 to 64 years. All subjects had signs of acneiform dermatoses. Pathologically changed areas were studied in comparison with normal skin in the same patient. The subjects were anesthetized and a piece of skin 3×3 mm2 in size was taken with the punch biopsy. After standard histological sample preparation, sections were stained with hematoxylin and eosin. The counting and morphometry of skin cell structures was carried out, the presence of lymphocytes, neutrophils, eosinophils, erythrocytes, vacuolized keratinocytes and their remnants in 6 fields of view in 3 histological slides from each patient was determined from each patient under an Axioskop 2 microscope with a ToupCam TP108500A camera and ToupView software (ToupTek , China). The criterion for the degree of destructive changes in the collagen fibers of the dermis was the determination of their thickness.Results. The morphological structure of the skin in acneiform dermatosis is characterized by a significant increase in the thickness of the spinous (93,7 [82,30; 96,35] microns), granular (28,4 [22,48; 34,44] microns) and corneum (37,8 [25,32; 46,24] microns) layers of the epidermis compared to the norm (66,1 [58,30; 69,42] microns; 6,2 [5,96; 6,63] microns; 10,6 [9,81; 11,26] microns, respectively). Near the sebaceous-follicular complex, inflammatory lymphohistiocytic infiltrates were found in the dermis, represented by lymphocytes (9,5±2,2 units p.f.v.), neutrophils (2,2±0,8 units p.f.v.), eosinophils (17,2±4,0 units p.f.v.), erythrocytes outside the vessels (6,8±1,1 units p.f.v.), vacuolated keratinocytes (22,1±5,7 units p.f.v.) and keratinocyte remnants (15,1±2,4 units p.f.v.) . The number of cells in the infiltrate significantly differed from the norm. On histological preparations of patients with acneiform dermatosis, thinning of the collagen fibers of the dermis (6,0 [4,79; 8,13] microns) was observed compared to unaffected skin (10,4 [10,01; 10,67] microns).Conclusions. Morphological changes in the skin with acneiform dermatosis are manifested by hypertrophic processes of the epidermis, thinning of the collagen fibers of the papillary dermis, the presence of leukocyte infiltrate and a violation of the integrity of the basement membrane of the sebaceous glands. These changes can lead to disruption of the integrity and functioning of the histo-hematic barrier of the skin and an increase in the area of spread of the inflammatory process.
{"title":"Morphofunctional changes in human skin with acneiform dermatoses","authors":"K. N. Pustovaya, G. A. Pyavchenko, N. Alexeeva, I. Kozlov, Z. V. Shamitko, V. A. Kovrigin, V. Nozdrin, S. Kuznetsov","doi":"10.18499/2225-7357-2022-11-4-33-40","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-33-40","url":null,"abstract":"The aim of this study was to study the morphofunctional changes in human skin with acneiform dermatoses.Material and methods. We studied the material obtained from 6 volunteers of both sexes aged 26 to 64 years. All subjects had signs of acneiform dermatoses. Pathologically changed areas were studied in comparison with normal skin in the same patient. The subjects were anesthetized and a piece of skin 3×3 mm2 in size was taken with the punch biopsy. After standard histological sample preparation, sections were stained with hematoxylin and eosin. The counting and morphometry of skin cell structures was carried out, the presence of lymphocytes, neutrophils, eosinophils, erythrocytes, vacuolized keratinocytes and their remnants in 6 fields of view in 3 histological slides from each patient was determined from each patient under an Axioskop 2 microscope with a ToupCam TP108500A camera and ToupView software (ToupTek , China). The criterion for the degree of destructive changes in the collagen fibers of the dermis was the determination of their thickness.Results. The morphological structure of the skin in acneiform dermatosis is characterized by a significant increase in the thickness of the spinous (93,7 [82,30; 96,35] microns), granular (28,4 [22,48; 34,44] microns) and corneum (37,8 [25,32; 46,24] microns) layers of the epidermis compared to the norm (66,1 [58,30; 69,42] microns; 6,2 [5,96; 6,63] microns; 10,6 [9,81; 11,26] microns, respectively). Near the sebaceous-follicular complex, inflammatory lymphohistiocytic infiltrates were found in the dermis, represented by lymphocytes (9,5±2,2 units p.f.v.), neutrophils (2,2±0,8 units p.f.v.), eosinophils (17,2±4,0 units p.f.v.), erythrocytes outside the vessels (6,8±1,1 units p.f.v.), vacuolated keratinocytes (22,1±5,7 units p.f.v.) and keratinocyte remnants (15,1±2,4 units p.f.v.) . The number of cells in the infiltrate significantly differed from the norm. On histological preparations of patients with acneiform dermatosis, thinning of the collagen fibers of the dermis (6,0 [4,79; 8,13] microns) was observed compared to unaffected skin (10,4 [10,01; 10,67] microns).Conclusions. Morphological changes in the skin with acneiform dermatosis are manifested by hypertrophic processes of the epidermis, thinning of the collagen fibers of the papillary dermis, the presence of leukocyte infiltrate and a violation of the integrity of the basement membrane of the sebaceous glands. These changes can lead to disruption of the integrity and functioning of the histo-hematic barrier of the skin and an increase in the area of spread of the inflammatory process.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78200408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-13DOI: 10.18499/2225-7357-2022-11-4-53-57
A. Epishkina, O. Deryabina, O. Tumutolova, D. Blinov, A. A. Makhrova, G. Brykin, D. N. Shimanovskii, E. Blinova
The aim of the study was to carry out a comparative morphological and immunohistochemical analysis of peripheral non-small cell lung cancer, which served as a source of a xenograft tumor, and tissues of the third generation of a tumor that developed in animals.Material and methods. We used 19 athymic BALB/c nu/nu mice, which were intraperitoneally injected with 1×106 CD8+ after sublethal irradiation in accordance with the humanization protocol. The transplantation of a tumor obtained from a 64-year-old patient was carried out three times consecutively. Samples of the original and xenograft tumors were automatically stained with hematoxylin and eosin, rabbit anti-CK7, anti-TTF, and anti-Ki67 antibodies. The evaluation of histological samples was carried out in accordance with the WHO recommendations (2015).Results. It has been established that a third-generation tumor developing in the body of athymic humanized mice retains the morphological and immunohistochemical features of the patient's original tumor. The described approach may be used in preclinical and personalized studies in fundamental pharmacology and molecular oncology.
{"title":"Morphological and immunohistochemical validation personalized patient-derived xenograft model of non-small cellular lung cancer","authors":"A. Epishkina, O. Deryabina, O. Tumutolova, D. Blinov, A. A. Makhrova, G. Brykin, D. N. Shimanovskii, E. Blinova","doi":"10.18499/2225-7357-2022-11-4-53-57","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-53-57","url":null,"abstract":"The aim of the study was to carry out a comparative morphological and immunohistochemical analysis of peripheral non-small cell lung cancer, which served as a source of a xenograft tumor, and tissues of the third generation of a tumor that developed in animals.Material and methods. We used 19 athymic BALB/c nu/nu mice, which were intraperitoneally injected with 1×106 CD8+ after sublethal irradiation in accordance with the humanization protocol. The transplantation of a tumor obtained from a 64-year-old patient was carried out three times consecutively. Samples of the original and xenograft tumors were automatically stained with hematoxylin and eosin, rabbit anti-CK7, anti-TTF, and anti-Ki67 antibodies. The evaluation of histological samples was carried out in accordance with the WHO recommendations (2015).Results. It has been established that a third-generation tumor developing in the body of athymic humanized mice retains the morphological and immunohistochemical features of the patient's original tumor. The described approach may be used in preclinical and personalized studies in fundamental pharmacology and molecular oncology.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89811698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-13DOI: 10.18499/2225-7357-2022-11-4-41-47
M. Mnikhovich, A. Fedorova, A. V. Romanov
Breast asymmetry is a polyetiological condition, which may be caused by congenital characteristics, developmental abnormalities, hormonal changes, traumas or surgery. The estimation of breast symmetry should be performed by the plastic surgeon while planning the augmentation or reduction mammoplasty as well as reconstructive surgery. Breast asymmetry is a widespread condition, according to some reports, it can be found in more than half of women. Anthropometric methods, as well as radiology methods, are used to diagnose and estimate breast asymmetry. There are many classification systems of breast asymmetry. The first ones were based on breast appearance or etiology of asymmetry, while modern classifications combine morphologic and etiologic principles. Today there is no conventional diagnostic protocol for breast asymmetry, where it would be listed, which breast parameters should be measured during breast asymmetry estimation. The first attempts were made in the middle of the XX century. Works of Penn, Smith and Westreich are considered to be basic in this field. Generally, relationships between major breast soft-tissue reference points (nipple, areola, submammary fold, lateral border) and bone structures (breastbone, jugular notch, clavicle) are estimated.Mathematic formulas for counting breast volume depending on its linear measurements were developed as well. Nowadays the importance of skeleto-muscular system state (the presence of scoliosis or rib cage deformation) estimation is emphasized, while these conditions can also cause breast asymmetry.
{"title":"Breast asymmetry classification and diagnostics","authors":"M. Mnikhovich, A. Fedorova, A. V. Romanov","doi":"10.18499/2225-7357-2022-11-4-41-47","DOIUrl":"https://doi.org/10.18499/2225-7357-2022-11-4-41-47","url":null,"abstract":"Breast asymmetry is a polyetiological condition, which may be caused by congenital characteristics, developmental abnormalities, hormonal changes, traumas or surgery. The estimation of breast symmetry should be performed by the plastic surgeon while planning the augmentation or reduction mammoplasty as well as reconstructive surgery. Breast asymmetry is a widespread condition, according to some reports, it can be found in more than half of women. Anthropometric methods, as well as radiology methods, are used to diagnose and estimate breast asymmetry. There are many classification systems of breast asymmetry. The first ones were based on breast appearance or etiology of asymmetry, while modern classifications combine morphologic and etiologic principles. Today there is no conventional diagnostic protocol for breast asymmetry, where it would be listed, which breast parameters should be measured during breast asymmetry estimation. The first attempts were made in the middle of the XX century. Works of Penn, Smith and Westreich are considered to be basic in this field. Generally, relationships between major breast soft-tissue reference points (nipple, areola, submammary fold, lateral border) and bone structures (breastbone, jugular notch, clavicle) are estimated.Mathematic formulas for counting breast volume depending on its linear measurements were developed as well. Nowadays the importance of skeleto-muscular system state (the presence of scoliosis or rib cage deformation) estimation is emphasized, while these conditions can also cause breast asymmetry.","PeriodicalId":15028,"journal":{"name":"Journal of Anatomy and Histopathology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74048866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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