Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758268
Yang Li, Yu Song, Yin Bai, Hua Lu, Jizhou Sun, C. Bian, J. Tong, S. Xia
a flow-through electrochemical sensor modified with renewable copper sensitive material for long-term nitrate measurement is demonstrated. The flow-through sensor based on conventional electrochemical electrodes was developed to implement long-term nitrate monitoring under a programmed operation protocol. The freshly deposited copper was employed as the electrocatalyst material for nitrate reduction. The corresponding cathodic amperometric values were measured to carry out nitrate determination. The renewal of copper sensing material was achieved by electrodepositing a fresh copper layer before each measurement, which is the key factor for sensitivity and reproducibility of long-term nitrate detection. The experimental results reveal that the sensor performed high sensitivity of 1.093 μA/mgL-1 for continuous nitrate determination. The linearity range for NO3- samples is from 0 to 12.1 mg L-1.
{"title":"A flow-through electrochemical sensor with renewable copper modified electrode for sensitive nitrate detection","authors":"Yang Li, Yu Song, Yin Bai, Hua Lu, Jizhou Sun, C. Bian, J. Tong, S. Xia","doi":"10.1109/NEMS.2016.7758268","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758268","url":null,"abstract":"a flow-through electrochemical sensor modified with renewable copper sensitive material for long-term nitrate measurement is demonstrated. The flow-through sensor based on conventional electrochemical electrodes was developed to implement long-term nitrate monitoring under a programmed operation protocol. The freshly deposited copper was employed as the electrocatalyst material for nitrate reduction. The corresponding cathodic amperometric values were measured to carry out nitrate determination. The renewal of copper sensing material was achieved by electrodepositing a fresh copper layer before each measurement, which is the key factor for sensitivity and reproducibility of long-term nitrate detection. The experimental results reveal that the sensor performed high sensitivity of 1.093 μA/mgL-1 for continuous nitrate determination. The linearity range for NO3- samples is from 0 to 12.1 mg L-1.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134317043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758259
C. Chuang, Wan-Ching Weng, W. Chang, Da-Huei Lee
We propose the use of simple and low cost piezoelectric based patch type sensors that ensure safety of the package while also detecting damage during shipment. A tear off sensor located at the top of the package can identify if the package has been opened while an impact sensor located at the bottom can monitor the damage suffered. The sensors are connected to a low power prototype read out device with a microcontroller (MCU) unit to analyze the data and a radio frequency (RF) module to transfer the data wirelessly. It would be viable to have an on-vehicle telematics device for collecting and analyzing data from multiple packages wirelessly so that it can be uploaded to the cloud, thus allowing for real time monitoring during transport. This logistics management model would aid in improving the quality of services provided by the logistics company while also earning consumer credibility. Thus, we believe that these patch type force sensors can be realistically implemented in logistics in the near future.
{"title":"Real-time monitoring via patch-type piezoelectric force sensors for Internet of Thing in logistics","authors":"C. Chuang, Wan-Ching Weng, W. Chang, Da-Huei Lee","doi":"10.1109/NEMS.2016.7758259","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758259","url":null,"abstract":"We propose the use of simple and low cost piezoelectric based patch type sensors that ensure safety of the package while also detecting damage during shipment. A tear off sensor located at the top of the package can identify if the package has been opened while an impact sensor located at the bottom can monitor the damage suffered. The sensors are connected to a low power prototype read out device with a microcontroller (MCU) unit to analyze the data and a radio frequency (RF) module to transfer the data wirelessly. It would be viable to have an on-vehicle telematics device for collecting and analyzing data from multiple packages wirelessly so that it can be uploaded to the cloud, thus allowing for real time monitoring during transport. This logistics management model would aid in improving the quality of services provided by the logistics company while also earning consumer credibility. Thus, we believe that these patch type force sensors can be realistically implemented in logistics in the near future.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130916644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758200
Zhuqing Wang, M. Kimura, N. Inomata, T. Ono
This research reports a multifunctional thermal biosensor that has thermocouple structures integrated with freestanding microfluidic channels for bio-sensing based on enzyme-catalyzed reactions. The free-standing SU-8 polymer-based microfluidic measurement chamber allows sensitive measurement of small volumes of liquid samples and reduces heat loss to improve the sensitivity. The fabricated thermocouple biosensor is capable of enzyme-catalyzed reaction detection for healthcare applications. Reactive enzymes are immobilized on microspheres packed in the chambers. The fabricated thermocouple biosensor shows a sensitivity of approximately 0.5 V/W and a thermal time constant of less than 100ms. The Seebeck-coefficient of thermocouple biosensor obtains a sensitivity of 1.1mV/°C with the measurement of IR thermometry. The results demonstrate that the sensor was capable of enzyme-catalyzed reaction detection for healthcare application.
{"title":"A freestanding microfluidic-based thermocouple biosensor for enzyme-catalyzed reaction analysis","authors":"Zhuqing Wang, M. Kimura, N. Inomata, T. Ono","doi":"10.1109/NEMS.2016.7758200","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758200","url":null,"abstract":"This research reports a multifunctional thermal biosensor that has thermocouple structures integrated with freestanding microfluidic channels for bio-sensing based on enzyme-catalyzed reactions. The free-standing SU-8 polymer-based microfluidic measurement chamber allows sensitive measurement of small volumes of liquid samples and reduces heat loss to improve the sensitivity. The fabricated thermocouple biosensor is capable of enzyme-catalyzed reaction detection for healthcare applications. Reactive enzymes are immobilized on microspheres packed in the chambers. The fabricated thermocouple biosensor shows a sensitivity of approximately 0.5 V/W and a thermal time constant of less than 100ms. The Seebeck-coefficient of thermocouple biosensor obtains a sensitivity of 1.1mV/°C with the measurement of IR thermometry. The results demonstrate that the sensor was capable of enzyme-catalyzed reaction detection for healthcare application.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115639948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758190
Lili Sun, Deyuan Zhang, Jun Cai
We present a straightforward technique to fabricate helical particles embedded with nanosilver particles aggregation using Spirulina platensis as scaffold, during which the nanosilver particles were controllably synthesized inside the biotemplate by the electroless deposition technique. The TEM of the as-prepared micro particles showed that nanosilver particles were universally distributed inside the biotemplate. The size of the intracellular nanosilver particles can be adjusted by controlling the reaction time. Helical particles prepared using this method are stable in deionized water. The intracellular synthesis mechanism of silver nanoparticles was analyzed. This technique can be generalized to other microorganism templates and different deposition materials that can be synthesized by electroless deposition technique. And the achieved highly-ordered nano particle aggregations have promising applications in various fields, such as nanocatalyst, wastewater treatment, biomedicine and anti-microbial materials.
{"title":"Controlled assembly of nanosilver particles inside bacterial cell scaffold","authors":"Lili Sun, Deyuan Zhang, Jun Cai","doi":"10.1109/NEMS.2016.7758190","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758190","url":null,"abstract":"We present a straightforward technique to fabricate helical particles embedded with nanosilver particles aggregation using Spirulina platensis as scaffold, during which the nanosilver particles were controllably synthesized inside the biotemplate by the electroless deposition technique. The TEM of the as-prepared micro particles showed that nanosilver particles were universally distributed inside the biotemplate. The size of the intracellular nanosilver particles can be adjusted by controlling the reaction time. Helical particles prepared using this method are stable in deionized water. The intracellular synthesis mechanism of silver nanoparticles was analyzed. This technique can be generalized to other microorganism templates and different deposition materials that can be synthesized by electroless deposition technique. And the achieved highly-ordered nano particle aggregations have promising applications in various fields, such as nanocatalyst, wastewater treatment, biomedicine and anti-microbial materials.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123868840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758252
N. Hayakawa, Kensuke Tsuchiya, T. Kakiuchi
We developed a micro-scale tensile fatigue test system, which can test specimens fabricated from bulk materials. In this system, a probe is attached to a micro-manipulator and the micro-manipulator enables accurate initial positioning and applying tensile stress. In this paper, we present the system and the result of tensile tests and tensile fatigue tests on the specimens which are made of coarse-grained magnesium alloy, AZ31. The tensile tests suggests that the system can apply accurate tensile stress to the micro-scale specimens and the tensile strength of micro-scale structures is much stronger than that of bulk materials. And the tensile fatigue test indicates that the system can apply intended cyclic tensile stress.
{"title":"Development of micro-scale tensile fatigue test system","authors":"N. Hayakawa, Kensuke Tsuchiya, T. Kakiuchi","doi":"10.1109/NEMS.2016.7758252","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758252","url":null,"abstract":"We developed a micro-scale tensile fatigue test system, which can test specimens fabricated from bulk materials. In this system, a probe is attached to a micro-manipulator and the micro-manipulator enables accurate initial positioning and applying tensile stress. In this paper, we present the system and the result of tensile tests and tensile fatigue tests on the specimens which are made of coarse-grained magnesium alloy, AZ31. The tensile tests suggests that the system can apply accurate tensile stress to the micro-scale specimens and the tensile strength of micro-scale structures is much stronger than that of bulk materials. And the tensile fatigue test indicates that the system can apply intended cyclic tensile stress.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123660055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758193
Andreas Loth, R. Forster
A novel disposable high pressure peristaltic micro pump has been developed, which allows a precise dosing of single volumes in the nanoliter range. The tube is integrated into a single use cartridge, which offers numerous advantages. A high pressure can be generated, while a small internal volume gives opportunity to handle expensive or rare fluids. Its small size allows the implementation into biochips or micro fluidic systems. The standalone solution can be connected to semi flexible tubes or pipes. The fluidic properties of the pump and the achievable maximum pressure have been determined. The fatigue resistance of the cartridge has been verified. Its simple structure offers the potential for mass production.
{"title":"Disposable high pressure peristaltic micro pump for standalone and on-chip applications","authors":"Andreas Loth, R. Forster","doi":"10.1109/NEMS.2016.7758193","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758193","url":null,"abstract":"A novel disposable high pressure peristaltic micro pump has been developed, which allows a precise dosing of single volumes in the nanoliter range. The tube is integrated into a single use cartridge, which offers numerous advantages. A high pressure can be generated, while a small internal volume gives opportunity to handle expensive or rare fluids. Its small size allows the implementation into biochips or micro fluidic systems. The standalone solution can be connected to semi flexible tubes or pipes. The fluidic properties of the pump and the achievable maximum pressure have been determined. The fatigue resistance of the cartridge has been verified. Its simple structure offers the potential for mass production.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123743617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758205
J. A. Rose, K. Komiya
We previously proposed, modeled, and experimentally validated a temperature-sensitive single-molecule DNA nanodevice that operates via competitive folding, as a potential platform for implementing a tunable thermal band-pass filter. Due to its peculiar hill-shaped efficiency profile, which differs markedly from the common sigmoidal melting curves observed for isolated DNA folding, this device could be used to control other molecular machines, and thus represents a promising biotechnological advance. Preliminary simulations established the basic feasibility of tuning the device for filter operation. However, the details of the complex dependencies of the peak temperature, width, and maximum value of the efficiency curve on the energetic stabilities of the individual device components, which is essential information for guiding directed design, remained unclear. In this work, an exact closed-form expression for predicting the peak temperature is derived and validated. The scaling behavior of this expression is then exploited to construct an effective algorithm for designing device implementations with target operating characteristics, thereby establishing the algorithmic tractability of tailored device design. This algorithm is then applied to produce a targeted filter design, with detailed simulations of device behavior. Finally, the application of the system model to folding error estimation is also discussed.
{"title":"Analysis and design of a single-molecule DNA nanodevice for thermal band-pass filters","authors":"J. A. Rose, K. Komiya","doi":"10.1109/NEMS.2016.7758205","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758205","url":null,"abstract":"We previously proposed, modeled, and experimentally validated a temperature-sensitive single-molecule DNA nanodevice that operates via competitive folding, as a potential platform for implementing a tunable thermal band-pass filter. Due to its peculiar hill-shaped efficiency profile, which differs markedly from the common sigmoidal melting curves observed for isolated DNA folding, this device could be used to control other molecular machines, and thus represents a promising biotechnological advance. Preliminary simulations established the basic feasibility of tuning the device for filter operation. However, the details of the complex dependencies of the peak temperature, width, and maximum value of the efficiency curve on the energetic stabilities of the individual device components, which is essential information for guiding directed design, remained unclear. In this work, an exact closed-form expression for predicting the peak temperature is derived and validated. The scaling behavior of this expression is then exploited to construct an effective algorithm for designing device implementations with target operating characteristics, thereby establishing the algorithmic tractability of tailored device design. This algorithm is then applied to produce a targeted filter design, with detailed simulations of device behavior. Finally, the application of the system model to folding error estimation is also discussed.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128320529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758204
L. D. Vreede, J. Berenschot, N. Tas, Wesley T. E. van den Beld, J. Loessberg-Zahl, A. V. D. Berg, J. Eijkel
We report on the manufacturing of nanopore through-holes by heating gold nanoparticles on a silicon oxide (SiO2) sheet, suspended in a silicon-rich nitride membrane (SiRN). Membrane patterning is performed using self-alignment by an internal shadow mask based process. A benefit of this approach is the ease at which downscaling of the lithographic features can be achieved. With a single alignment, a shadow mask is etched and metal is deposited. The nanopore through hole is then created after heating. In this paper this scalable technique is applied to create non-buckled membranes by combining the compressive and tensile stress components in a SiO2/SiRN bilayer. Theory on the bilayer stresses is given in order to characterize the buckling. The nanopore through holes are characterized using ionic current measurements and electron microscopy techniques.
{"title":"Nanopores created using an internal shadowmask process","authors":"L. D. Vreede, J. Berenschot, N. Tas, Wesley T. E. van den Beld, J. Loessberg-Zahl, A. V. D. Berg, J. Eijkel","doi":"10.1109/NEMS.2016.7758204","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758204","url":null,"abstract":"We report on the manufacturing of nanopore through-holes by heating gold nanoparticles on a silicon oxide (SiO2) sheet, suspended in a silicon-rich nitride membrane (SiRN). Membrane patterning is performed using self-alignment by an internal shadow mask based process. A benefit of this approach is the ease at which downscaling of the lithographic features can be achieved. With a single alignment, a shadow mask is etched and metal is deposited. The nanopore through hole is then created after heating. In this paper this scalable technique is applied to create non-buckled membranes by combining the compressive and tensile stress components in a SiO2/SiRN bilayer. Theory on the bilayer stresses is given in order to characterize the buckling. The nanopore through holes are characterized using ionic current measurements and electron microscopy techniques.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122012174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758249
Yoshitaka Yoshizumi, M. Yokokawa, H. Suzuki
This paper proposes a self-deformable structure consisting of tethered multiple micromotors. Pt/Au segmented microrods formed by electrodeposition were used as the micromotors. To fabricate flexible tethers, several approaches were tried including metal backbones, metallized DNA strands, and polymers stacked by layer-by-layer (LbL) self-assembly. For the metal backbones, shadowing effect of sputtering was used to deposit the backbones only on the half side of the micromotors. As the metallized DNA strands, λ-DNA strands with Au particle chain was formed. LbL self-assembled polymer bilayers was fabricated with a combination of poly(acrylic acid) (PAA) / poly(allylamine hydrochloride) (PAH) to encapsulate and tether the micromotors. The tethered structures were fabricated by removing a sacrificial Ag segments after the formation of the tethers. To evaluate the flexibility of the tethers, Brownian fluctuation of the open angle was traced. Micromotors tethered with the Au-metallized DNA strand and the cross-linked LbL polymers showed larger fluctuations (> 30°), whereas no fluctuation was observed with micromotors tethered with Au backbones. Furthermore, the close-to-open motion was observed with the LbL self-assembled polymer tethers.
{"title":"Self-deformable micro/nanomotors with organic-inorganic hybrid structures","authors":"Yoshitaka Yoshizumi, M. Yokokawa, H. Suzuki","doi":"10.1109/NEMS.2016.7758249","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758249","url":null,"abstract":"This paper proposes a self-deformable structure consisting of tethered multiple micromotors. Pt/Au segmented microrods formed by electrodeposition were used as the micromotors. To fabricate flexible tethers, several approaches were tried including metal backbones, metallized DNA strands, and polymers stacked by layer-by-layer (LbL) self-assembly. For the metal backbones, shadowing effect of sputtering was used to deposit the backbones only on the half side of the micromotors. As the metallized DNA strands, λ-DNA strands with Au particle chain was formed. LbL self-assembled polymer bilayers was fabricated with a combination of poly(acrylic acid) (PAA) / poly(allylamine hydrochloride) (PAH) to encapsulate and tether the micromotors. The tethered structures were fabricated by removing a sacrificial Ag segments after the formation of the tethers. To evaluate the flexibility of the tethers, Brownian fluctuation of the open angle was traced. Micromotors tethered with the Au-metallized DNA strand and the cross-linked LbL polymers showed larger fluctuations (> 30°), whereas no fluctuation was observed with micromotors tethered with Au backbones. Furthermore, the close-to-open motion was observed with the LbL self-assembled polymer tethers.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121727835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2016-04-17DOI: 10.1109/NEMS.2016.7758187
Yu-Dong Ma, Wen-Hsin Chang, Chih-Hung Wang, Shih‐Yuan Liu, Wen-Hsiang Yen, K. Luo, H. You, Jiunn-Jong Wu, Mel S. Lee, Gwo-Bin Lee
Digital loop-mediated amplification (LAMP) is an isothermal, quantitative DNA amplification approach which has high sensitivity and specificity and could be used to analyze extremely low amount of DNA within a short period of time. In this study, an integrated self-driven microfluidic chip was proposed herein for performing a digital LAMP process for vancomycin-resistant Enterococcus (VRE) diagnosis. The entire bacterial diagnosis could be automatically performed by capillary forces through the new polydimethylsiloxane (PDMS) surface treatment without using external pumps. Moreover, digitized droplets could be separated from each other by normally-close valves such that it could be quantitated. The contact angle of the treated PDMS surface was measured to be only 24°, which was the lowest value in literatures. This is the first time that a rapid (within 1 hour), simple, and hydrophilic PDMS surface modification method has been reported, which could be used in self-driven microfluidic devices for digital LAMP. This device may become a promising tool for clinical diagnosis and point-of-care applications.
{"title":"A self-driven microfluidic chip through a rapid surface modification of PDMS and its application for digital loop-mediated amplification (LAMP)","authors":"Yu-Dong Ma, Wen-Hsin Chang, Chih-Hung Wang, Shih‐Yuan Liu, Wen-Hsiang Yen, K. Luo, H. You, Jiunn-Jong Wu, Mel S. Lee, Gwo-Bin Lee","doi":"10.1109/NEMS.2016.7758187","DOIUrl":"https://doi.org/10.1109/NEMS.2016.7758187","url":null,"abstract":"Digital loop-mediated amplification (LAMP) is an isothermal, quantitative DNA amplification approach which has high sensitivity and specificity and could be used to analyze extremely low amount of DNA within a short period of time. In this study, an integrated self-driven microfluidic chip was proposed herein for performing a digital LAMP process for vancomycin-resistant Enterococcus (VRE) diagnosis. The entire bacterial diagnosis could be automatically performed by capillary forces through the new polydimethylsiloxane (PDMS) surface treatment without using external pumps. Moreover, digitized droplets could be separated from each other by normally-close valves such that it could be quantitated. The contact angle of the treated PDMS surface was measured to be only 24°, which was the lowest value in literatures. This is the first time that a rapid (within 1 hour), simple, and hydrophilic PDMS surface modification method has been reported, which could be used in self-driven microfluidic devices for digital LAMP. This device may become a promising tool for clinical diagnosis and point-of-care applications.","PeriodicalId":150449,"journal":{"name":"2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131483516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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